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3hay

Crystal structure of a substrate-bound full H/ACA RNP from Pyrococcus furiosus

Method: X-RAY DIFFRACTION
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Probable tRNA pseudouridine synthase B

Pyrococcus furiosus

UniProt Q7LWY0

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 4 RNA 2 Small nucleolar rnp gar1-like protein × 1 （Q8U029） Ribosome biogenesis protein Nop10 × 1 （Q8U1R4） 50S ribosomal protein L7Ae × 1 （Q8U160） H/ACA RNA × 1 5'-R(*AP*UP*AP*AP*UP*UP*(FHU)P*GP*AP*CP*UP*CP*AP*A)-3' × 1 ZINC ION × 1 Consistent with all polymers

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
2ey4 2ey4 2ey4 2hvy 2rfk 3hax 3hay 3hjw 3hjy 3lwo 3lwp 3lwq

15 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name TRUB_PYRFU
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–340； UniProt 1–340

Small nucleolar rnp gar1-like protein

Pyrococcus furiosus

UniProt Q8U029

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 4 RNA 2 Probable tRNA pseudouridine synthase B × 1 （Q7LWY0） Ribosome biogenesis protein Nop10 × 1 （Q8U1R4） 50S ribosomal protein L7Ae × 1 （Q8U160） H/ACA RNA × 1 5'-R(*AP*UP*AP*AP*UP*UP*(FHU)P*GP*AP*CP*UP*CP*AP*A)-3' × 1 ZINC ION × 1 Consistent with all polymers

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
2hvy 2rfk 3hay 3mqk

4 assemblies

View Construct and Data Evidence
UniProt name Q8U029_PYRFU
Isoform —
PDB entities 2
Chains and sequence ranges Author chain B； PDBConstruct 1–104； UniProt 1–104

Ribosome biogenesis protein Nop10

Pyrococcus furiosus

UniProt Q8U1R4

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 4 RNA 2 Probable tRNA pseudouridine synthase B × 1 （Q7LWY0） Small nucleolar rnp gar1-like protein × 1 （Q8U029） 50S ribosomal protein L7Ae × 1 （Q8U160） H/ACA RNA × 1 5'-R(*AP*UP*AP*AP*UP*UP*(FHU)P*GP*AP*CP*UP*CP*AP*A)-3' × 1 ZINC ION × 1 Consistent with all polymers

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
2ey4 2ey4 2ey4 2hvy 2rfk 3hax 3hay 3hjw 3hjy 3lwo 3lwp 3lwq

15 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name NOP10_PYRFU
Isoform —
PDB entities 3
Chains and sequence ranges Author chain C； PDBConstruct 1–60； UniProt 1–60

50S ribosomal protein L7Ae

Pyrococcus furiosus

UniProt Q8U160

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 4 RNA 2 Probable tRNA pseudouridine synthase B × 1 （Q7LWY0） Small nucleolar rnp gar1-like protein × 1 （Q8U029） Ribosome biogenesis protein Nop10 × 1 （Q8U1R4） H/ACA RNA × 1 5'-R(*AP*UP*AP*AP*UP*UP*(FHU)P*GP*AP*CP*UP*CP*AP*A)-3' × 1 ZINC ION × 1 Consistent with all polymers

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
2hvy 3hax 3hay 3hjw 3lwo 3lwp 3lwq 3lwr 3lwv 3nmu 3nmu 3nmu

29 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name RL7A_PYRFU
Isoform —
PDB entities 4
Chains and sequence ranges Author chain D； PDBConstruct 3–124； UniProt 2–123

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id3hay
Deposition date deposition_date2009-05-03
Structure title titleCrystal structure of a substrate-bound full H/ACA RNP from Pyrococcus furiosus
Keywords keywords;H/ACA, guide RNA, RNA-protein complex, pseudouridine synthase, Isomerase, tRNA processing, Ribonucleoprotein, Ribosome biogenesis, rRNA processing, Ribosomal protein, RNA-binding, ISOMERASE-BIOSYNTHETIC PROTEIN-RNA COMPLEX ;; ISOMERASE/BIOSYNTHETIC PROTEIN/RNA
Experimental Method methodX-RAY DIFFRACTION
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

3hay__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

3hay__assembly_1__model_1 | I(q)

10-2 10-1 106 107 108 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

3hay__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）31.70 Å
Rg (electron density)31.20 Å
Total Rg31.78 Å
Atom count6327
Residues657
Excluded volume107630 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 3hay__assembly_1__model_1 hexameric （6） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (7)

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7. Citations (1)