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3hop

Structure of the actin-binding domain of human filamin A

Method: X-RAY DIFFRACTION Dmax: 74.4 Å Quality: EXCELLENT
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Filamin-A

Homo sapiens

UniProt P21333

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count Chain A； UniProt 2–269 Fragment：Actin-binding domain PO4 PHOSPHATE ION × 1 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；20% PEG 4000, 0.2M lithium sulfate, 0.1M Tris HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K Resolution 2.30 Å R-free 0.288
2 Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count Chain B； UniProt 2–269 Fragment：Actin-binding domain PO4 PHOSPHATE ION × 1 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；20% PEG 4000, 0.2M lithium sulfate, 0.1M Tris HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K Resolution 2.30 Å R-free 0.288

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 3HOP Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
2AAV Solution NMR structure of Filamin A domain 17 Deposited 2005-07-14 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1862–1955（94 aa） Fragment：Filamin A domain 17
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.7；293 K；Ionic strength (raw mmCIF value) 50mM；Pressure 1
NMR sample composition GAMV(1863-1956FLNA), U-13C, U-15N, NMR sample 0.3mM; 50mM sodium phosphate, 10mM dithiothreitol; 95% H2O, 5% D2O | 95% H2O/5% D2O
Resolution not provided
2BP3 Crystal structure of Filamin A domain 17 and GPIb alpha cytoplasmic domain complex Deposited 2005-04-18 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1863–1956（94 aa） Fragment：ROD DOMAIN, RESIDUES 1863-1956
Not recorded GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.2；1.75M AMMONIUM PHOSPHATE, PH 8.2, AFTER MICROSEEDING: 1.25M AMMONIUM SULFATE PH 8.2
Resolution 2.32 Å R-free 0.256
2BP3 Crystal structure of Filamin A domain 17 and GPIb alpha cytoplasmic domain complex Deposited 2005-04-18 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 1863–1956（94 aa） Fragment：ROD DOMAIN, RESIDUES 1863-1956
Not recorded GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.2；1.75M AMMONIUM PHOSPHATE, PH 8.2, AFTER MICROSEEDING: 1.25M AMMONIUM SULFATE PH 8.2
Resolution 2.32 Å R-free 0.256
2BRQ Crystal structure of the filamin A repeat 21 complexed with the integrin beta7 cytoplasmic tail peptide Deposited 2005-05-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 2236–2329（94 aa） Fragment：ROD DOMAIN, RESIDUES 2236-2329
Chain B 2236–2329（94 aa） Fragment：ROD DOMAIN, RESIDUES 2236-2329
Not recorded GSH Glutathione × 2 GOL GLYCEROL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 5.5；1.26 M SODIUM CITRATE, 0.1 M CITRIC ACID PH 5.5
Resolution 2.10 Å R-free 0.243
2J3S Crystal structure of the human filamin A Ig domains 19 to 21 Deposited 2006-08-23 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 2045–2329（285 aa） Fragment：IMMUNOGLOBULIN-LIKE DOMAINS 19 TO 21, RESIDUES 2045-2329
Not recorded BR BROMIDE ION × 1 DIO 1,4-DIETHYLENE DIOXIDE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.1；PROTEIN WAS CRYSTALLIZED FROM 1.6M AMMONIUM SULPHATE, 0.1M CITRIC ACID PH 6.1, 10% DIOXANE.
Resolution 2.50 Å R-free 0.297
2J3S Crystal structure of the human filamin A Ig domains 19 to 21 Deposited 2006-08-23 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 2045–2329（285 aa） Fragment：IMMUNOGLOBULIN-LIKE DOMAINS 19 TO 21, RESIDUES 2045-2329
Not recorded BR BROMIDE ION × 1 DIO 1,4-DIETHYLENE DIOXIDE × 2 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.1；PROTEIN WAS CRYSTALLIZED FROM 1.6M AMMONIUM SULPHATE, 0.1M CITRIC ACID PH 6.1, 10% DIOXANE.
Resolution 2.50 Å R-free 0.297
2JF1 CRYSTAL STRUCTURE OF THE FILAMIN A REPEAT 21 COMPLEXED WITH THE INTEGRIN BETA2 CYTOPLASMIC TAIL PEPTIDE Deposited 2007-01-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 2236–2329（94 aa） Fragment：IG 21, RESIDUES 2236-2329
Not recorded GOL GLYCEROL × 3 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.6；1.6-1.58 M AMMONIUM SULPHATE, 0.1 M NA-ACETATE PH 4.6
Resolution 2.20 Å R-free 0.278
2K3T Solution Structure of IG-Like Domain 23 from Human Filamin A Deposited 2008-05-16 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 2427–2522（96 aa） Fragment：Ig-like domain 23 of Filamin A (UNP residues 2427-2522)
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.8；298 K；Ionic strength (raw mmCIF value) 150；Pressure ambient
NMR sample composition 1 mM [U-13C; U-15N] FLNA23, 20 mM Sodium phosphate, 150 mM Sodium chloride, 1 mM DTT, 2 mM Sodium azide, 93% H2O/7% D2O | 93% H2O/7% D2O
Resolution not provided
2K7P Filamin A Ig-like domains 16-17 Deposited 2008-08-19 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1772–1956（185 aa） Fragment：Filamin 16-17, UNP residues 1772-1956
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.8；303 K；Ionic strength (raw mmCIF value) 100；Pressure ambient
NMR sample composition 1mM [U-13C; U-15N] FLNa16-17, 50mM sodium phosphate, 100mM potassium chloride, 1mM DTT, 2mM sodium azide, 2mM EDTA, 93% H2O/7% D2O | 93% H2O/7% D2O
Resolution not provided
2K7Q Filamin A Ig-like domains 18-19 Deposited 2008-08-19 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1954–2141（188 aa） Fragment：Filamin 18-19, UNP residues 1954-2141
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.8；303 K；Ionic strength (raw mmCIF value) 100；Pressure ambient
NMR sample composition 0.8mM [U-13C; U-15N] FLNa18-19, 50mM sodium phosphate, 100mM potassium chloride, 1mM DTT, 2mM sodium azide, 93% H2O/7% D2O | 93% H2O/7% D2O
Resolution not provided
2MTP The structure of Filamin repeat 21 bound to integrin Deposited 2014-08-28 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 2236–2330（95 aa） Fragment：UNP residues 2236-2330
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.4；298 K；Ionic strength (raw mmCIF value) 25；Pressure ambient
NMR sample composition 1 mM [U-100% 13C; U-100% 15N; U-80% 2H] protein, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
2W0P Crystal structure of the filamin A repeat 21 complexed with the migfilin peptide Deposited 2008-08-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 2236–2329（94 aa） Fragment：IG-21, RESIDUES 2236-2329
Chain B 2236–2329（94 aa） Fragment：IG-21, RESIDUES 2236-2329
Not recorded SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions 1.7M (NH4)2SO4, 5% 2-PROPANOL
Resolution 1.90 Å R-free 0.239
2WFN Filamin A actin binding domain Deposited 2009-04-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–278（278 aa） Fragment：ACTIN BINDING DOMAIN, RESIDUES 1-278
Chain B 1–278（278 aa） Fragment：ACTIN BINDING DOMAIN, RESIDUES 1-278
Not recorded SO4 SULFATE ION × 7 X-RAY DIFFRACTION
X-ray crystallization conditions 1.7 M LITHIUM SULFATE, 0.1 M HEPES PH 7.5
Resolution 3.20 Å R-free 0.243
3CNK Crystal Structure of the dimerization domain of human filamin A Deposited 2008-03-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 2559–2647（89 aa） Fragment：Filamin 24, repeat 24
Chain B 2559–2647（89 aa） Fragment：Filamin 24, repeat 24
Not recorded SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.2；293 K；0.1M Tris, 30% PEG 3350, 0.2M Lithium Sulfate, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 8.20
Resolution 1.65 Å R-free 0.219
3HOC Structure of the actin-binding domain of human filamin A mutant E254K Deposited 2009-06-02 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 2–269（268 aa） Fragment：Actin-binding domain
Mutation：E254K PO4 PHOSPHATE ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.8；293 K；20% PEG 4000, 0.2M lithium sulfate, 0.1M Tris HCl, 5mM DTT, pH 8.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.30 Å R-free 0.263
3HOC Structure of the actin-binding domain of human filamin A mutant E254K Deposited 2009-06-02 Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 2–269（268 aa） Fragment：Actin-binding domain
Mutation：E254K PO4 PHOSPHATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.8；293 K；20% PEG 4000, 0.2M lithium sulfate, 0.1M Tris HCl, 5mM DTT, pH 8.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.30 Å R-free 0.263
3HOC Structure of the actin-binding domain of human filamin A mutant E254K Deposited 2009-06-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 2–269（268 aa） Fragment：Actin-binding domain
Chain B 2–269（268 aa） Fragment：Actin-binding domain
Mutation：E254K Mutation：E254K PO4 PHOSPHATE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.8；293 K；20% PEG 4000, 0.2M lithium sulfate, 0.1M Tris HCl, 5mM DTT, pH 8.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.30 Å R-free 0.263
3HOR Structure of the actin-binding domain of human filamin A (reduced) Deposited 2009-06-03 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 2–269（268 aa） Fragment：Actin-binding domain
Not recorded PO4 PHOSPHATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；20% PEG 4000, 0.2M lithium sulfate, 0.1M Tris HCl, 5mM DTT, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.70 Å R-free 0.294
3HOR Structure of the actin-binding domain of human filamin A (reduced) Deposited 2009-06-03 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 2–269（268 aa） Fragment：Actin-binding domain
Not recorded PO4 PHOSPHATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；20% PEG 4000, 0.2M lithium sulfate, 0.1M Tris HCl, 5mM DTT, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.70 Å R-free 0.294
3ISW Crystal structure of filamin-A immunoglobulin-like repeat 21 bound to an N-terminal peptide of CFTR Deposited 2009-08-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 2236–2329（94 aa） Fragment：UNP residues 2236-2329
Chain B 2236–2329（94 aa） Fragment：UNP residues 2236-2329
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；0.1M Bis-Tris Propane pH 7.0, 60% Tacsimate, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.80 Å R-free 0.298
3RGH Structure of filamin A immunoglobulin-like repeat 10 from Homo sapiens Deposited 2011-04-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1158–1252（95 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) ACT ACETATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；0.2 M ammonium acetate, 25%(w/v) PEG 3350, 0.1 M Bis-tris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.44 Å R-free 0.279
3RGH Structure of filamin A immunoglobulin-like repeat 10 from Homo sapiens Deposited 2011-04-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1158–1252（95 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) ACT ACETATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；0.2 M ammonium acetate, 25%(w/v) PEG 3350, 0.1 M Bis-tris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.44 Å R-free 0.279
4M9P Crystal structure of the human filamin A Ig-like domains 3-5 Deposited 2013-08-15 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 478–766（289 aa） Fragment：domains 3-5 (UNP residues 478-766)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；298 K；0.1 M sodium malonate, 12% w/v PEG3350, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 1.72 Å R-free 0.243
4P3W Crystal structure of the human filamin A Ig-like domains 20-21 in complex with migfilin peptide Deposited 2014-03-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 2152–2329（178 aa） Fragment：Filamin repeats 20 and 21, residues 2152-2329
Not recorded SO4 SULFATE ION × 1 PR PRASEODYMIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；295 K；0.1 M MES pH 6, 1.9 M (NH4)2SO4, 0.1 M (CH3CO2)3Pr
Resolution 2.00 Å R-free 0.226
4P3W Crystal structure of the human filamin A Ig-like domains 20-21 in complex with migfilin peptide Deposited 2014-03-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 2152–2329（178 aa） Fragment：Filamin repeats 20 and 21, residues 2152-2329
Not recorded SO4 SULFATE ION × 1 PR PRASEODYMIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；295 K；0.1 M MES pH 6, 1.9 M (NH4)2SO4, 0.1 M (CH3CO2)3Pr
Resolution 2.00 Å R-free 0.226
4P3W Crystal structure of the human filamin A Ig-like domains 20-21 in complex with migfilin peptide Deposited 2014-03-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain E 2152–2329（178 aa） Fragment：Filamin repeats 20 and 21, residues 2152-2329
Not recorded SO4 SULFATE ION × 1 PR PRASEODYMIUM ION × 1 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；295 K；0.1 M MES pH 6, 1.9 M (NH4)2SO4, 0.1 M (CH3CO2)3Pr
Resolution 2.00 Å R-free 0.226
4P3W Crystal structure of the human filamin A Ig-like domains 20-21 in complex with migfilin peptide Deposited 2014-03-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain F 2152–2329（178 aa） Fragment：Filamin repeats 20 and 21, residues 2152-2329
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；295 K；0.1 M MES pH 6, 1.9 M (NH4)2SO4, 0.1 M (CH3CO2)3Pr
Resolution 2.00 Å R-free 0.226
4P3W Crystal structure of the human filamin A Ig-like domains 20-21 in complex with migfilin peptide Deposited 2014-03-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 5 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain D 2152–2329（178 aa） Fragment：Filamin repeats 20 and 21, residues 2152-2329
Not recorded PR PRASEODYMIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；295 K；0.1 M MES pH 6, 1.9 M (NH4)2SO4, 0.1 M (CH3CO2)3Pr
Resolution 2.00 Å R-free 0.226
4P3W Crystal structure of the human filamin A Ig-like domains 20-21 in complex with migfilin peptide Deposited 2014-03-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 6 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 2152–2329（178 aa） Fragment：Filamin repeats 20 and 21, residues 2152-2329
Not recorded SO4 SULFATE ION × 1 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；295 K；0.1 M MES pH 6, 1.9 M (NH4)2SO4, 0.1 M (CH3CO2)3Pr
Resolution 2.00 Å R-free 0.226
5XR1 Structure of FLN IG21 domain in complex with C-terminal peptide of beta-2 Deposited 2017-06-07 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 2228–2321（94 aa）
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.2；298 K；Ionic strength (raw mmCIF value) 150；Pressure 1
NMR sample composition 300 uM [U-13C; U-15N] FLN IG21 domain, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
6D8C Cryo-EM structure of FLNaABD E254K bound to phalloidin-stabilized F-actin Deposited 2018-04-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 13 PDB declaration: tridecameric
Chain A 1–278（278 aa） Fragment：UNP residues 1-278
Chain B 1–278（278 aa） Fragment：UNP residues 1-278
Chain C 1–278（278 aa） Fragment：UNP residues 1-278
Chain D 1–278（278 aa） Fragment：UNP residues 1-278
Chain E 1–278（278 aa） Fragment：UNP residues 1-278
Mutation：E254K Mutation：E254K Mutation：E254K Mutation：E254K Mutation：E254K ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.54 Å
6EW1 Crystal structure of the Filamin A Ig-like domains 3-5 mutant P637Q Deposited 2017-11-03 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 478–766（289 aa）
Mutation：P637Q No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；0.05 M ammonium citrate, 18 % PEG 3350
Resolution 2.31 Å R-free 0.273
7SC4 filamin complex-1 Deposited 2021-09-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 2236–2329（94 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；289.15 K；2 M Ammonium Sulfate, 5% 2-propanol
Resolution 1.85 Å R-free 0.261
7SC4 filamin complex-1 Deposited 2021-09-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain B 2236–2329（94 aa）
Not recorded SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；289.15 K；2 M Ammonium Sulfate, 5% 2-propanol
Resolution 1.85 Å R-free 0.261
7SFT Filamin complex-2 Deposited 2021-10-04 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 2236–2330（95 aa）
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.4；298 K；Ionic strength (raw mmCIF value) 61；Pressure 1
NMR sample composition 1 mM [U-100% 13C; U-100% 15N] filamin Ig21, 2 mM Integrin alpha-IIb light chain, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 1 mM [U-15N; U-2H] filamin Ig21, 2 mM Integrin alpha-IIb light chain, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 2 mM Integrin alpha-IIb light chain, 0.05 mM GST-filamin-Ig21, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
9LWX Crystal structure of the Filamin A repeat 21 Deposited 2025-02-17 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain D 2236–2329（94 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.2 M Sodium acetate trihydrate, 0.1 M Sodium cacodylate trihydrate, pH 6.5, 30% w/ Polyethylene glycol (PEG) 8,000
Resolution 2.29 Å R-free 0.268
9LWX Crystal structure of the Filamin A repeat 21 Deposited 2025-02-17 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 2236–2329（94 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.2 M Sodium acetate trihydrate, 0.1 M Sodium cacodylate trihydrate, pH 6.5, 30% w/ Polyethylene glycol (PEG) 8,000
Resolution 2.29 Å R-free 0.268
9LWX Crystal structure of the Filamin A repeat 21 Deposited 2025-02-17 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 2236–2329（94 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.2 M Sodium acetate trihydrate, 0.1 M Sodium cacodylate trihydrate, pH 6.5, 30% w/ Polyethylene glycol (PEG) 8,000
Resolution 2.29 Å R-free 0.268
9LWX Crystal structure of the Filamin A repeat 21 Deposited 2025-02-17 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 2236–2329（94 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.2 M Sodium acetate trihydrate, 0.1 M Sodium cacodylate trihydrate, pH 6.5, 30% w/ Polyethylene glycol (PEG) 8,000
Resolution 2.29 Å R-free 0.268
9LXG Filamin A repeat 21 in complex with LARP4 Ala269-Asn281 peptide Deposited 2025-02-18 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 2236–2330（95 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；1.0 M Lithium sulfate, 0.1 M Sodium citrate pH 5.6, 0.5 M Ammonium sulfate
Resolution 2.46 Å R-free 0.259

25 other PDB entries and 40 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name FLNA_HUMAN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 5–272； UniProt 2–269 Author chain B； PDBConstruct 5–272； UniProt 2–269

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 3hop

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 3hop
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2. Structure Basics 2. Structure Basics

Entry ID entry_id3hop
Deposition date deposition_date2009-06-03
Structure title titleStructure of the actin-binding domain of human filamin A
Keywords keywords;Calponin homology domain, actin binding domain, Acetylation, Actin-binding, Alternative splicing, Cytoplasm, Cytoskeleton, Disease mutation, Phosphoprotein, Polymorphism, Structural Protein ;; STRUCTURAL PROTEIN
Experimental Method methodX-RAY DIFFRACTION
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier24.79 Å
Radius of gyration Rg (electron density) rg_electron23.61 Å
Forward intensity I(0) i044170500.00
Molecular weight molecular_weight51388.0 kDa
Excluded volume excluded_volume64407 ų
Envelope volume envelope_volume79096 ų
Hydration-shell volume shell_volume27565 ų
Envelope diameter envelope_diameter78.8 Å
Shell Rg shell_rg30.95 Å
Envelope Rg envelope_rg23.62 Å
Shape Rg shape_rg23.62 Å
Total Rg total_rg24.48 Å
Total atoms total_atoms3612
Residues n_residues457
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax74.4 Å
Rg (real space) rg_real24.60 Å
Rg uncertainty (real space) rg_real_error0.43 Å
I(0) (real space) i0_real4.4170e+07
I(0) uncertainty (real space) i0_real_error5.8470e+05
Rg (reciprocal space) rg_reciprocal24.64 Å
I(0) (reciprocal space) i0_reciprocal44170000.0000
Solution quality estimate total_estimate0.9123
Solution quality rating solution_quality EXCELLENT a EXCELLENT solution
P(r) peaks n_peaks2
Primary peak position r_peak_primary34.2 Å
Skewness Skewness skewness0.059
Kurtosis Kurtosis kurtosis-0.640
Angular range angular_range— – 0.3200 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha10600000.0000
Real-space data points n_real_points64
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.958; Stabil: 0.999; Sysdev: 1.000; Positv: 1.000; Valcen: 0.993; Smooth: 0.991
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (3)

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7. Fold Classification (SCOP + CATH) 8 domains

SCOP 2.08 (4 domains)

Domain ID domain_idd3hopa1
Class classa — All alpha proteins
Fold Fold folda.40 — CH domain-like
Superfamily Superfamily superfamilya.40.1 — Calponin-homology domain, CH-domain
Family Family familya.40.1.0 — automated matches
Domain ID domain_idd3hopa2
Class classa — All alpha proteins
Fold Fold folda.40 — CH domain-like
Superfamily Superfamily superfamilya.40.1 — Calponin-homology domain, CH-domain
Family Family familya.40.1.0 — automated matches
Domain ID domain_idd3hopb1
Class classa — All alpha proteins
Fold Fold folda.40 — CH domain-like
Superfamily Superfamily superfamilya.40.1 — Calponin-homology domain, CH-domain
Family Family familya.40.1.0 — automated matches
Domain ID domain_idd3hopb2
Class classa — All alpha proteins
Fold Fold folda.40 — CH domain-like
Superfamily Superfamily superfamilya.40.1 — Calponin-homology domain, CH-domain
Family Family familya.40.1.0 — automated matches

CATH v4.4 (4 domains)

Domain ID domain_id3hopA01
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology418 — Actin-binding Protein, T-fimbrin; domain 1
Homologous superfamily homologous superfamily10 — Calponin-like domain
Domain ID domain_id3hopA02
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology418 — Actin-binding Protein, T-fimbrin; domain 1
Homologous superfamily homologous superfamily10 — Calponin-like domain
Domain ID domain_id3hopB01
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology418 — Actin-binding Protein, T-fimbrin; domain 1
Homologous superfamily homologous superfamily10 — Calponin-like domain
Domain ID domain_id3hopB02
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology418 — Actin-binding Protein, T-fimbrin; domain 1
Homologous superfamily homologous superfamily10 — Calponin-like domain
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8. Citations (1)

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9. Files and Curves (10)