Outer-membrane lipoprotein carrier protein
Escherichia coli
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain A； UniProt 22–203 | Fragment：Residues 22-203 | No other associated polymer | X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 4.4；277 K；25% PEG 1000, 20% PEG 8000, 0.1 M sodium acetate, dehydrated over 1M ammonium sulfate, pH 4.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K | Resolution 1.65 Å R-free 0.243 |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 3KSN | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1IWL Crystal Structure of the Lipoprotein Localization Factor, LolA Deposited 2002-05-17 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
22–203（182 aa）
|
Not recorded | ZN ZINC ION × 3 MG MAGNESIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；277 K；PEG8000, sodium cacodilate, zinc acetate, glycerol, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 1.65 Å R-free 0.249 |
| 1UA8 Crystal structure of the lipoprotein localization factor, LolA Deposited 2003-03-04 | Different construct Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
22–203（182 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；PEG1500, Tris-HCl, Glycerol, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.90 Å R-free 0.259 |
| 2ZPC Crystal structure of the R43L mutant of LolA in the closed form Deposited 2008-07-10 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
22–203（182 aa）
|
Mutation：R43L | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；15% PEG 3350, 50mM MES (pH 6.5), 20% glycerol , VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.35 Å R-free 0.275 |
| 2ZPD Crystal structure of the R43L mutant of LolA in the open form Deposited 2008-07-10 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
22–203（182 aa）
|
Mutation：R43L | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；15% PEG 3350, 50mM MES (pH 6.5), 20% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.85 Å R-free 0.231 |
| 6F3Z Complex of E. coli LolA and periplasmic domain of LolC Deposited 2017-11-29 | Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
22–203（182 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；288.15 K；45% w/v Poly(acrylic acid sodium salt) 2100, 100 mM HEPES pH 6.5
|
Resolution 2.00 Å R-free 0.249 |
| 6F3Z Complex of E. coli LolA and periplasmic domain of LolC Deposited 2017-11-29 | Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain D
22–203（182 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；288.15 K；45% w/v Poly(acrylic acid sodium salt) 2100, 100 mM HEPES pH 6.5
|
Resolution 2.00 Å R-free 0.249 |
| 6FHM Crystal structure of the F47E mutant of the lipoprotein localization factor, LolA Deposited 2018-01-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
22–203（182 aa）
Chain B
22–203（182 aa）
|
Mutation：F47E Mutation：F47E | GOL GLYCEROL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；288.15 K；13% PEG 8000, 20% glycerol
|
Resolution 2.39 Å R-free 0.269 |
| 7ARM LolCDE in complex with lipoprotein and LolA Deposited 2020-10-25 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric |
Chain A
20–203（184 aa）
|
Not recorded | Z41 (2S)-3-hydroxypropane-1,2-diyl dihexadecanoate × 1 PLM PALMITIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.8；20 mM Tris-HCl, pH 7.8, 150 mM NaCl and 0.05% LMNG
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 7Z6W Complex of E. coli LolA and lipoprotein Deposited 2022-03-14 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
22–203（182 aa）
|
Not recorded | IG7 [(2~{S})-3-[(2~{S})-3-azanyl-2-(hexadecanoylamino)-3-oxidanylidene-propyl]sulfanyl-2-hexadecanoyloxy-propyl] hexadecanoate × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；288.15 K；2.1 M DL-Malic acid pH 6.0
|
Resolution 1.84 Å R-free 0.216 |
| 7Z6X Complex of E. coli LolA R43L mutant and lipoprotein Deposited 2022-03-14 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
22–203（182 aa）
|
Mutation：R43L | IG7 [(2~{S})-3-[(2~{S})-3-azanyl-2-(hexadecanoylamino)-3-oxidanylidene-propyl]sulfanyl-2-hexadecanoyloxy-propyl] hexadecanoate × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；288.15 K；50 mM bis-Tris pH 6.0, 30% PEG 3350
|
Resolution 2.06 Å R-free 0.272 |
9 other PDB entries and 10 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | LOLA_ECOLI |
| Isoform | — |
| PDB entities | 1 |
| Chains and sequence ranges | Author chain A； PDBConstruct 2–183； UniProt 22–203 |
