SAXSdb — 4bl0

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1. Protein Identity and Related Structures Protein Identity & Related Structures

CELL CYCLE ARREST PROTEIN BUB3

SACCHAROMYCES CEREVISIAE

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 3	CHECKPOINT SERINE/THREONINE-PROTEIN KINASE BUB1 × 1 （P41695） SPINDLE POLE BODY COMPONENT SPC105 × 1 （P53148） MAGNESIUM ION × 1 water × 3	Consistent with protein count
2	Protein heterocomplex	Heteromer	Protein 3	CHECKPOINT SERINE/THREONINE-PROTEIN KINASE BUB1 × 1 （P41695） SPINDLE POLE BODY COMPONENT SPC105 × 1 （P53148） MAGNESIUM ION × 1 water × 3	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	BUB3_YEAST
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–341； UniProt 1–341 Author chain D； PDBConstruct 1–341； UniProt 1–341

CHECKPOINT SERINE/THREONINE-PROTEIN KINASE BUB1

SACCHAROMYCES CEREVISIAE

UniProt P41695

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 3	CELL CYCLE ARREST PROTEIN BUB3 × 1 （P26449） SPINDLE POLE BODY COMPONENT SPC105 × 1 （P53148） MAGNESIUM ION × 1 water × 3	Consistent with protein count
2	Protein heterocomplex	Heteromer	Protein 3	CELL CYCLE ARREST PROTEIN BUB3 × 1 （P26449） SPINDLE POLE BODY COMPONENT SPC105 × 1 （P53148） MAGNESIUM ION × 1 water × 3	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	BUB1_YEAST
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain B； PDBConstruct 5–75； UniProt 289–359 Author chain E； PDBConstruct 5–75； UniProt 289–359

SPINDLE POLE BODY COMPONENT SPC105

OrganismNot specified

UniProt P53148

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 3	CELL CYCLE ARREST PROTEIN BUB3 × 1 （P26449） CHECKPOINT SERINE/THREONINE-PROTEIN KINASE BUB1 × 1 （P41695） MAGNESIUM ION × 1 water × 3	Consistent with protein count
2	Protein heterocomplex	Heteromer	Protein 3	CELL CYCLE ARREST PROTEIN BUB3 × 1 （P26449） CHECKPOINT SERINE/THREONINE-PROTEIN KINASE BUB1 × 1 （P41695） MAGNESIUM ION × 1 water × 3	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	SP105_YEAST
Isoform	—
PDB entities	3
Chains and sequence ranges	Author chain C； PDBConstruct 1–19； UniProt 165–183 Author chain F； PDBConstruct 1–19； UniProt 165–183

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	4bl0
Deposition date deposition_date	2013-04-30
Structure title title	Crystal structure of yeast Bub3-Bub1 bound to phospho-Spc105
Keywords keywords	;CELL CYCLE, BUBR1, MAD3, RAE1, GLE2, GLEBS, MAD1, MAD2, SPINDLE ASSEMBLY CHECKPOINT, KNL1, CASC5, SPC7, BLINKIN, KINETOCHORE, MITOSIS, CELL DIVISION, ANEUPLOIDY ;; CELL CYCLE
Experimental Method method	X-RAY DIFFRACTION

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

4bl0__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

4bl0__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

4bl0__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	21.05 Å
Rg (electron density)	19.81 Å
Total Rg	20.77 Å
Atom count	3027
Residues	387
Excluded volume	54191 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`4bl0__assembly_1__model_1`	trimeric （3）	Success	4.1.3-1-20251215 (887e7ef)	View Download
2	1	`4bl0__assembly_2__model_1`	trimeric （3）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (5)

Entity 1 Entity 1 polymer

Description description	CELL CYCLE ARREST PROTEIN BUB3
Molecular weight formula_weight	38483.5 kDa
Source method src_method	man
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,D
Atom count atom_count	6408

MQIVQIEQAPKDYISDIKIIPSKSLLLITSWDGSLTVYKFDIQAKNVDLLQSLRYKHPLLCCNFIDNTDLQIYVGTVQGEILKVDLIGSPSFQALTNNEANLGICRICKYGDDKLIAASWDGLIEVIDPRNYGDGVIAVKNLNSNNTKVKNKIFTMDTNSSRLIVGMNNSQVQWFRLPLCEDDNGTIEESGLKYQIRDVALLPKEQEGYACSSIDGRVAVEFFDDQGDDYNSSKRFAFRCHRLNLKDTNLAYPVNSIEFSPRHKFLYTAGSDGIISCWNLQTRKKIKNFAKFNEDSVVKIACSDNILCLATSDDTFKTNAAIDQTIELNASSIYIIFDYEN

Source Source

Source organism organism	SACCHAROMYCES CEREVISIAE
Common name common_name	;BAKER'S YEAST ;
NCBI taxonomy ID ncbi_taxonomy_id	4932
Host organism host_organism	ESCHERICHIA COLI

Entity 2 Entity 2 polymer

Description description	CHECKPOINT SERINE/THREONINE-PROTEIN KINASE BUB1
Molecular weight formula_weight	8782.9 kDa
EC 编号 ec_number	2.7.11.1
Source method src_method	man
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	B,E

PLGSNKKTSIYADQKQSNNPVYKLINTPGRKPERIVFNFNLIYPENDEEFNTEEILAMIKGLYKVQRRGKKHTED

Source Source

Source organism organism	SACCHAROMYCES CEREVISIAE
Common name common_name	;BAKER'S YEAST ;
NCBI taxonomy ID ncbi_taxonomy_id	4932
Host organism host_organism	ESCHERICHIA COLI

Entity 3 Entity 3 polymer

Description description	SPINDLE POLE BODY COMPONENT SPC105
Molecular weight formula_weight	2349.6 kDa
Source method src_method	syn
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	C,F

DPTSMEMTEVFPRSIRQKN

Entity 4 Entity 4 non-polymer

Description description	MAGNESIUM ION
Molecular weight formula_weight	24.3 kDa
Source method src_method	syn
Copy count entity_count	2

Entity 5 Entity 5 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	356

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6. Fold Classification (SCOP + CATH) 4 domains

CATH v4.4 (4 domains)

Domain ID domain_id	4bl0A00
Class class	2 — Mainly Beta
Architecture architecture	130 — 7 Propeller
Topology topology	10 — Methylamine Dehydrogenase; Chain H
Homologous superfamily homologous superfamily	10 — YVTN repeat-like/Quinoprotein amine dehydrogenase

Domain ID domain_id	4bl0B00
Class class	6 — Special
Architecture architecture	10 — Helix non-globular
Topology topology	20 — Arc Repressor Mutant, subunit A
Homologous superfamily homologous superfamily	170 —

Domain ID domain_id	4bl0D00
Class class	2 — Mainly Beta
Architecture architecture	130 — 7 Propeller
Topology topology	10 — Methylamine Dehydrogenase; Chain H
Homologous superfamily homologous superfamily	10 — YVTN repeat-like/Quinoprotein amine dehydrogenase

Domain ID domain_id	4bl0E00
Class class	6 — Special
Architecture architecture	10 — Helix non-globular
Topology topology	20 — Arc Repressor Mutant, subunit A
Homologous superfamily homologous superfamily	170 —

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Unit-cell length a length_a	138.740 Å
Unit-cell length b length_b	57.900 Å
Unit-cell length c length_c	118.670 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	102.53 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	8

Space group space_group	C 1 2 1
Space-group number int_tables_number	5
Crystal system crystal_system	—

Resolution resolution	1.950 Å
Low-resolution limit resolution_low	46.882 Å
R factor r_factor	0.1800
R_work r_work	0.1794
R_free r_free	0.1922

Crystal structure of yeast Bub3-Bub1 bound to phospho-Spc105

1. Protein Identity and Related Structures Protein Identity & Related Structures

CELL CYCLE ARREST PROTEIN BUB3

State in the Current Structure

Other States of the Same Protein in the Database

CHECKPOINT SERINE/THREONINE-PROTEIN KINASE BUB1

State in the Current Structure

Other States of the Same Protein in the Database

SPINDLE POLE BODY COMPONENT SPC105

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

4bl0__assembly_1__model_1

4bl0__assembly_1__model_1 | I(q)

4bl0__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (5)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 polymer

Polymer Polymer

Source Source

Entity 3 Entity 3 polymer

Polymer Polymer

Entity 4 Entity 4 non-polymer

Entity 5 Entity 5 water

6. Fold Classification (SCOP + CATH) 4 domains

CATH v4.4 (4 domains)

7. Citations (1)

Crystal structure of yeast Bub3-Bub1 bound to phospho-Spc105

1. Protein Identity and Related Structures Protein Identity & Related Structures

CELL CYCLE ARREST PROTEIN BUB3

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

CHECKPOINT SERINE/THREONINE-PROTEIN KINASE BUB1

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

SPINDLE POLE BODY COMPONENT SPC105

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

4bl0__assembly_1__model_1

4bl0__assembly_1__model_1 | I(q)

4bl0__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (5)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 polymer

Polymer Polymer

Source Source

Entity 3 Entity 3 polymer

Polymer Polymer

Entity 4 Entity 4 non-polymer

Entity 5 Entity 5 water

6. Fold Classification (SCOP + CATH) 4 domains

CATH v4.4 (4 domains)

7. Citations (1)