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4jd2

Crystal structure of Bos taurus Arp2/3 complex binding with Mus musculus GMF

Method: X-RAY DIFFRACTION Dmax: 147.7 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Actin-related protein 3

OrganismNot specified

UniProt P61157

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count Chain A； UniProt 1–418 Not recorded Actin-related protein 2 × 1 （A7MB62） Actin-related protein 2/3 complex subunit 1B × 1 （Q58CQ2） Actin-related protein 2/3 complex subunit 2 × 1 （Q3MHR7） Actin-related protein 2/3 complex subunit 3 × 1 （Q3T035） Actin-related protein 2/3 complex subunit 4 × 1 （Q148J6） Actin-related protein 2/3 complex subunit 5 × 1 （Q3SYX9） Glia maturation factor gamma × 1 （Q9ERL7） CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 PG4 TETRAETHYLENE GLYCOL × 2 X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions Resolution 3.08 Å R-free 0.248

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4JD2 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1K8K Crystal Structure of Arp2/3 Complex Deposited 2001-10-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain A 1–418（418 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；PEG 8000, KSCN, Hepes at pH 7.5, VAPOR DIFFUSION, HANGING DROP at 298K
Resolution 2.00 Å R-free 0.251
1TYQ Crystal structure of Arp2/3 complex with bound ATP and calcium Deposited 2004-07-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain A 1–418（418 aa）
Not recorded CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；PEG 8000, potassium thiocyanate, Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.55 Å R-free 0.272
1U2V Crystal structure of Arp2/3 complex with bound ADP and calcium Deposited 2004-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain A 1–418（418 aa）
Not recorded CA CALCIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；Hepes, potassium thiocyanate, PEG 8000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.55 Å R-free 0.258
1U2V Crystal structure of Arp2/3 complex with bound ADP and calcium Deposited 2004-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain A 1–418（418 aa）
Not recorded CA CALCIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；Hepes, potassium thiocyanate, PEG 8000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.55 Å R-free 0.258
2P9I Crystal Structure of bovine Arp2/3 Complex co-crystallized with ADP and crosslinked with gluteraldehyde Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain A 1–418（418 aa）
Not recorded CA CALCIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；7.5% PEG 3350 50mM HEPES 100mM KSCN 10% Sucrose, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.46 Å R-free 0.261
2P9K Crystal structure of bovine Arp2/3 complex co-crystallized with ATP and crosslinked with glutaraldehyde Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain A 1–418（418 aa）
Not recorded CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；278 K；7.5% PEG 3350, 50mM HEPES, 100mM KSCN, 10% Sucrose, VAPOR DIFFUSION, HANGING DROP, temperature 278K
Resolution 2.59 Å R-free 0.260
2P9L Crystal Structure of bovine Arp2/3 complex Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain A 1–418（418 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；7.5% PEG3350 50mM HEPES 100mM KSCN 10% Sucrose, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.65 Å R-free 0.266
2P9N Crystal Structure of bovine Arp2/3 complex co-crystallized with ADP Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain A 1–418（418 aa）
Not recorded CA CALCIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；6% PEG 8000 100mM HEPES 100mM KSCN, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.85 Å R-free 0.272
2P9P Crystal Structure of bovine Arp2/3 complex co-crystallized with ADP Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain A 1–418（418 aa）
Not recorded CA CALCIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；7.5% PEG 3350 50mM HEPES 100mM KSCN 10% Sucrose, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.90 Å R-free 0.288
2P9S Structure of bovine Arp2/3 complex co-crystallized with ATP/Mg2+ Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain A 1–418（418 aa）
Not recorded MG MAGNESIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；7.5% PEG3350 50mM HEPES 100mM KSCN 10% Sucrose, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.68 Å R-free 0.261
2P9U Crystal structure of bovine Arp2/3 complex co-crystallized with AMP-PNP and calcium Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain A 1–418（418 aa）
Not recorded CA CALCIUM ION × 2 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；7.5% PEG3350 50mM HEPES 100mM KSCN 10% Sucrose, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.75 Å R-free 0.265
3DXK Structure of Bos Taurus Arp2/3 Complex with Bound Inhibitor CK0944636 Deposited 2008-07-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain A 1–418（418 aa）
Not recorded N23 N-[2-(2-methyl-1H-indol-3-yl)ethyl]thiophene-2-carboxamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；8% PEG 8000, 50 mM HEPES, pH 7.5, 100 mM KSCN, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.70 Å R-free 0.262
3DXM Structure of Bos taurus Arp2/3 Complex with Bound Inhibitor CK0993548 Deposited 2008-07-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain A 1–418（418 aa）
Not recorded N24 (2S)-2-(3-bromophenyl)-3-(5-chloro-2-hydroxyphenyl)-1,3-thiazolidin-4-one × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；8% PEG 8000, 50 mM HEPES, pH 7.5,100 mM KSCN, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.85 Å R-free 0.258
3RSE Structural and biochemical characterization of two binding sites for nucleation promoting factor WASp-VCA on Arp2/3 complex Deposited 2011-05-02 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–418（418 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；100 mM HEPES 7.5, 8% PEG 8000, 200 mM KCl, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.65 Å R-free 0.259
3UKR Crystal structure of Bos taurus Arp2/3 complex with bound inhibitor CK-666 Deposited 2011-11-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain A 1–418（418 aa）
Not recorded CKH 2-fluoro-N-[2-(2-methyl-1H-indol-3-yl)ethyl]benzamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；0.1M Hepes (pH 7.5), 0.2M KSCN, 16% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.48 Å R-free 0.261
3UKU Structure of Arp2/3 complex with bound inhibitor CK-869 Deposited 2011-11-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain A 1–418（418 aa）
Not recorded C69 (2S)-2-(3-bromophenyl)-3-(2,4-dimethoxyphenyl)-1,3-thiazolidin-4-one × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；278 K；8% PEG 8000, 50 mM HEPES, pH 7.5, 100 mM KSCN, VAPOR DIFFUSION, HANGING DROP, temperature 278K
Resolution 2.75 Å R-free 0.301
3ULE Structure of Bos taurus Arp2/3 complex with bound inhibitor CK-869 and ATP Deposited 2011-11-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain A 1–418（418 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 2 C69 (2S)-2-(3-bromophenyl)-3-(2,4-dimethoxyphenyl)-1,3-thiazolidin-4-one × 1 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；277 K；8% PEG 8000, 50 mM HEPES, pH 7.5,100 mM KSCN, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.50 Å R-free 0.274
4XEI Orthorhombic isomorph of bovine Arp2/3 complex Deposited 2014-12-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain A 1–418（418 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.4；277 K；Ethanol 10%, 1,4-butanediol 15%, 100 mM Tris 7.4
Resolution 3.87 Å R-free 0.329
4XF2 Tetragonal structure of Arp2/3 complex Deposited 2014-12-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain A 1–418（418 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；100 mM Tris 7.0, 15% ethanol
Resolution 5.00 Å R-free 0.303
4XF2 Tetragonal structure of Arp2/3 complex Deposited 2014-12-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain T 1–418（418 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；100 mM Tris 7.0, 15% ethanol
Resolution 5.00 Å R-free 0.303
6DEC Crystal structure of Bos taurus Arp2/3 complex binding with C-terminus of Homo sapiens SPIN90 Deposited 2018-05-11 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–418（418 aa）
Not recorded CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；50 mM HEPES, pH 7.5, 5% PEG3350, 50 mM L-Proline, 0.5 mM ATP, 0.5 mM calcium chloride, 1 mM DTT
Resolution 4.60 Å R-free 0.314
6DEC Crystal structure of Bos taurus Arp2/3 complex binding with C-terminus of Homo sapiens SPIN90 Deposited 2018-05-11 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain H 1–418（418 aa）
Not recorded CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；50 mM HEPES, pH 7.5, 5% PEG3350, 50 mM L-Proline, 0.5 mM ATP, 0.5 mM calcium chloride, 1 mM DTT
Resolution 4.60 Å R-free 0.314
7JPN Cryo-EM structure of Arpin-bound Arp2/3 complex Deposited 2020-08-09 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 3–415（413 aa）
Not recorded MG MAGNESIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE；Grids were manually blotted for 3 seconds with Whatman 41 filter paper and manually plunged using a Leica EM CPC manual plunger.
Resolution 3.24 Å
7T5Q Cryo-EM Structure of a Transition State of Arp2/3 Complex Activation Deposited 2021-12-13 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 10 PDB declaration: decameric
Chain A 1–418（418 aa）
Not recorded MG MAGNESIUM ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE；Grids were manually blotted for 3 seconds with Whatman 41 filter paper and manually plunged using a Leica EM CPC manual plunger.
Resolution 3.40 Å
7TPT Single-particle Cryo-EM structure of Arp2/3 complex at branched-actin junction. Deposited 2022-01-26 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 36 PDB declaration: 36-meric
Chain A 1–418（418 aa）
Not recorded MG MAGNESIUM ION × 16 ADP ADENOSINE-5'-DIPHOSPHATE × 16 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8TAH Cryo-EM structure of Cortactin-bound to Arp2/3 complex Deposited 2023-06-27 Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–418（418 aa）
Not recorded MG MAGNESIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7；10 mM imidazole pH 7.0, 50 mM KCl, 2 mM MgCl2, 1 mM EGTA
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.89 Å
9DLX Bovine Arp2/3 complex with N-WASP CA bound to Arp3 and Arp2-ArpC1 Deposited 2024-09-11 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric
Chain A 3–414（412 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.91 Å
9DLZ Bovine Arp2/3 complex with N-WASP CA bound to Arp3 Deposited 2024-09-11 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric
Chain A 3–413（411 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
9EAM Coronin-7 CA bound to Arp2/3 complex Deposited 2024-11-11 Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–418（418 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.97 Å
9M5E Structure of SPIN90 dimer-Arp2/3 complex-nucleated actin filament (singlet complex) Deposited 2025-03-05 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric
Chain A 1–418（418 aa）
Not recorded MG MAGNESIUM ION × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.55 Å
9M64 Structure of SPIN90 dimer-Arp2/3 complexes-nucleated actin filaments (Doublet Complex) Deposited 2025-03-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain A 1–418（418 aa）
Chain a 1–418（418 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 12 MG MAGNESIUM ION × 10 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å

28 other PDB entries and 31 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name ARP3_BOVIN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–418； UniProt 1–418

Actin-related protein 2

OrganismNot specified

UniProt A7MB62

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count Chain B； UniProt 1–394 Not recorded Actin-related protein 3 × 1 （P61157） Actin-related protein 2/3 complex subunit 1B × 1 （Q58CQ2） Actin-related protein 2/3 complex subunit 2 × 1 （Q3MHR7） Actin-related protein 2/3 complex subunit 3 × 1 （Q3T035） Actin-related protein 2/3 complex subunit 4 × 1 （Q148J6） Actin-related protein 2/3 complex subunit 5 × 1 （Q3SYX9） Glia maturation factor gamma × 1 （Q9ERL7） CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 PG4 TETRAETHYLENE GLYCOL × 2 X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions Resolution 3.08 Å R-free 0.248

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4JD2 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1K8K Crystal Structure of Arp2/3 Complex Deposited 2001-10-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain B 1–394（394 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；PEG 8000, KSCN, Hepes at pH 7.5, VAPOR DIFFUSION, HANGING DROP at 298K
Resolution 2.00 Å R-free 0.251
2P9I Crystal Structure of bovine Arp2/3 Complex co-crystallized with ADP and crosslinked with gluteraldehyde Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain B 1–394（394 aa）
Not recorded CA CALCIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；7.5% PEG 3350 50mM HEPES 100mM KSCN 10% Sucrose, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.46 Å R-free 0.261
2P9K Crystal structure of bovine Arp2/3 complex co-crystallized with ATP and crosslinked with glutaraldehyde Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain B 1–394（394 aa）
Not recorded CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；278 K；7.5% PEG 3350, 50mM HEPES, 100mM KSCN, 10% Sucrose, VAPOR DIFFUSION, HANGING DROP, temperature 278K
Resolution 2.59 Å R-free 0.260
2P9L Crystal Structure of bovine Arp2/3 complex Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain B 1–394（394 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；7.5% PEG3350 50mM HEPES 100mM KSCN 10% Sucrose, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.65 Å R-free 0.266
2P9N Crystal Structure of bovine Arp2/3 complex co-crystallized with ADP Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain B 1–394（394 aa）
Not recorded CA CALCIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；6% PEG 8000 100mM HEPES 100mM KSCN, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.85 Å R-free 0.272
2P9P Crystal Structure of bovine Arp2/3 complex co-crystallized with ADP Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain B 1–394（394 aa）
Not recorded CA CALCIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；7.5% PEG 3350 50mM HEPES 100mM KSCN 10% Sucrose, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.90 Å R-free 0.288
2P9S Structure of bovine Arp2/3 complex co-crystallized with ATP/Mg2+ Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain B 1–394（394 aa）
Not recorded MG MAGNESIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；7.5% PEG3350 50mM HEPES 100mM KSCN 10% Sucrose, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.68 Å R-free 0.261
2P9U Crystal structure of bovine Arp2/3 complex co-crystallized with AMP-PNP and calcium Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain B 1–394（394 aa）
Not recorded CA CALCIUM ION × 2 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；7.5% PEG3350 50mM HEPES 100mM KSCN 10% Sucrose, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.75 Å R-free 0.265
3DXK Structure of Bos Taurus Arp2/3 Complex with Bound Inhibitor CK0944636 Deposited 2008-07-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain B 1–394（394 aa）
Not recorded N23 N-[2-(2-methyl-1H-indol-3-yl)ethyl]thiophene-2-carboxamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；8% PEG 8000, 50 mM HEPES, pH 7.5, 100 mM KSCN, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.70 Å R-free 0.262
3DXM Structure of Bos taurus Arp2/3 Complex with Bound Inhibitor CK0993548 Deposited 2008-07-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain B 1–394（394 aa）
Not recorded N24 (2S)-2-(3-bromophenyl)-3-(5-chloro-2-hydroxyphenyl)-1,3-thiazolidin-4-one × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；8% PEG 8000, 50 mM HEPES, pH 7.5,100 mM KSCN, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.85 Å R-free 0.258
3RSE Structural and biochemical characterization of two binding sites for nucleation promoting factor WASp-VCA on Arp2/3 complex Deposited 2011-05-02 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 1–394（394 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；100 mM HEPES 7.5, 8% PEG 8000, 200 mM KCl, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.65 Å R-free 0.259
3UKR Crystal structure of Bos taurus Arp2/3 complex with bound inhibitor CK-666 Deposited 2011-11-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain B 1–394（394 aa）
Not recorded CKH 2-fluoro-N-[2-(2-methyl-1H-indol-3-yl)ethyl]benzamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；0.1M Hepes (pH 7.5), 0.2M KSCN, 16% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.48 Å R-free 0.261
3UKU Structure of Arp2/3 complex with bound inhibitor CK-869 Deposited 2011-11-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain B 1–394（394 aa）
Not recorded C69 (2S)-2-(3-bromophenyl)-3-(2,4-dimethoxyphenyl)-1,3-thiazolidin-4-one × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；278 K；8% PEG 8000, 50 mM HEPES, pH 7.5, 100 mM KSCN, VAPOR DIFFUSION, HANGING DROP, temperature 278K
Resolution 2.75 Å R-free 0.301
3ULE Structure of Bos taurus Arp2/3 complex with bound inhibitor CK-869 and ATP Deposited 2011-11-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain B 1–394（394 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 2 C69 (2S)-2-(3-bromophenyl)-3-(2,4-dimethoxyphenyl)-1,3-thiazolidin-4-one × 1 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；277 K；8% PEG 8000, 50 mM HEPES, pH 7.5,100 mM KSCN, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.50 Å R-free 0.274
4XEI Orthorhombic isomorph of bovine Arp2/3 complex Deposited 2014-12-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain B 1–394（394 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.4；277 K；Ethanol 10%, 1,4-butanediol 15%, 100 mM Tris 7.4
Resolution 3.87 Å R-free 0.329
4XF2 Tetragonal structure of Arp2/3 complex Deposited 2014-12-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain B 1–394（394 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；100 mM Tris 7.0, 15% ethanol
Resolution 5.00 Å R-free 0.303
4XF2 Tetragonal structure of Arp2/3 complex Deposited 2014-12-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain U 1–394（394 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；100 mM Tris 7.0, 15% ethanol
Resolution 5.00 Å R-free 0.303
6DEC Crystal structure of Bos taurus Arp2/3 complex binding with C-terminus of Homo sapiens SPIN90 Deposited 2018-05-11 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 1–394（394 aa）
Not recorded CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；50 mM HEPES, pH 7.5, 5% PEG3350, 50 mM L-Proline, 0.5 mM ATP, 0.5 mM calcium chloride, 1 mM DTT
Resolution 4.60 Å R-free 0.314
6DEC Crystal structure of Bos taurus Arp2/3 complex binding with C-terminus of Homo sapiens SPIN90 Deposited 2018-05-11 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain I 1–394（394 aa）
Not recorded CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；50 mM HEPES, pH 7.5, 5% PEG3350, 50 mM L-Proline, 0.5 mM ATP, 0.5 mM calcium chloride, 1 mM DTT
Resolution 4.60 Å R-free 0.314
7JPN Cryo-EM structure of Arpin-bound Arp2/3 complex Deposited 2020-08-09 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 4–380（377 aa）
Not recorded MG MAGNESIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE；Grids were manually blotted for 3 seconds with Whatman 41 filter paper and manually plunged using a Leica EM CPC manual plunger.
Resolution 3.24 Å
7T5Q Cryo-EM Structure of a Transition State of Arp2/3 Complex Activation Deposited 2021-12-13 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 10 PDB declaration: decameric
Chain B 1–394（394 aa）
Not recorded MG MAGNESIUM ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE；Grids were manually blotted for 3 seconds with Whatman 41 filter paper and manually plunged using a Leica EM CPC manual plunger.
Resolution 3.40 Å
7TPT Single-particle Cryo-EM structure of Arp2/3 complex at branched-actin junction. Deposited 2022-01-26 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 36 PDB declaration: 36-meric
Chain B 1–394（394 aa）
Not recorded MG MAGNESIUM ION × 16 ADP ADENOSINE-5'-DIPHOSPHATE × 16 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8TAH Cryo-EM structure of Cortactin-bound to Arp2/3 complex Deposited 2023-06-27 Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 1–394（394 aa）
Not recorded MG MAGNESIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7；10 mM imidazole pH 7.0, 50 mM KCl, 2 mM MgCl2, 1 mM EGTA
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.89 Å
9DLX Bovine Arp2/3 complex with N-WASP CA bound to Arp3 and Arp2-ArpC1 Deposited 2024-09-11 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric
Chain B 1–390（390 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.91 Å
9DLZ Bovine Arp2/3 complex with N-WASP CA bound to Arp3 Deposited 2024-09-11 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric
Chain B 1–390（390 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
9EAM Coronin-7 CA bound to Arp2/3 complex Deposited 2024-11-11 Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 1–394（394 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.97 Å
9M5E Structure of SPIN90 dimer-Arp2/3 complex-nucleated actin filament (singlet complex) Deposited 2025-03-05 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric
Chain B 1–394（394 aa）
Not recorded MG MAGNESIUM ION × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.55 Å
9M64 Structure of SPIN90 dimer-Arp2/3 complexes-nucleated actin filaments (Doublet Complex) Deposited 2025-03-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain B 1–394（394 aa）
Chain b 1–394（394 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 12 MG MAGNESIUM ION × 10 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å

26 other PDB entries and 28 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name ARP2_BOVIN
Isoform —
PDB entities 2
Chains and sequence ranges Author chain B； PDBConstruct 1–394； UniProt 1–394

Actin-related protein 2/3 complex subunit 1B

OrganismNot specified

UniProt Q58CQ2

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count Chain C； UniProt 1–372 Not recorded Actin-related protein 3 × 1 （P61157） Actin-related protein 2 × 1 （A7MB62） Actin-related protein 2/3 complex subunit 2 × 1 （Q3MHR7） Actin-related protein 2/3 complex subunit 3 × 1 （Q3T035） Actin-related protein 2/3 complex subunit 4 × 1 （Q148J6） Actin-related protein 2/3 complex subunit 5 × 1 （Q3SYX9） Glia maturation factor gamma × 1 （Q9ERL7） CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 PG4 TETRAETHYLENE GLYCOL × 2 X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions Resolution 3.08 Å R-free 0.248

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4JD2 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1K8K Crystal Structure of Arp2/3 Complex Deposited 2001-10-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain C 1–372（372 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；PEG 8000, KSCN, Hepes at pH 7.5, VAPOR DIFFUSION, HANGING DROP at 298K
Resolution 2.00 Å R-free 0.251
2P9I Crystal Structure of bovine Arp2/3 Complex co-crystallized with ADP and crosslinked with gluteraldehyde Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain C 1–372（372 aa）
Not recorded CA CALCIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；7.5% PEG 3350 50mM HEPES 100mM KSCN 10% Sucrose, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.46 Å R-free 0.261
2P9K Crystal structure of bovine Arp2/3 complex co-crystallized with ATP and crosslinked with glutaraldehyde Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain C 1–372（372 aa）
Not recorded CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；278 K；7.5% PEG 3350, 50mM HEPES, 100mM KSCN, 10% Sucrose, VAPOR DIFFUSION, HANGING DROP, temperature 278K
Resolution 2.59 Å R-free 0.260
2P9L Crystal Structure of bovine Arp2/3 complex Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain C 1–372（372 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；7.5% PEG3350 50mM HEPES 100mM KSCN 10% Sucrose, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.65 Å R-free 0.266
2P9N Crystal Structure of bovine Arp2/3 complex co-crystallized with ADP Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain C 1–372（372 aa）
Not recorded CA CALCIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；6% PEG 8000 100mM HEPES 100mM KSCN, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.85 Å R-free 0.272
2P9P Crystal Structure of bovine Arp2/3 complex co-crystallized with ADP Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain C 1–372（372 aa）
Not recorded CA CALCIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；7.5% PEG 3350 50mM HEPES 100mM KSCN 10% Sucrose, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.90 Å R-free 0.288
2P9S Structure of bovine Arp2/3 complex co-crystallized with ATP/Mg2+ Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain C 1–372（372 aa）
Not recorded MG MAGNESIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；7.5% PEG3350 50mM HEPES 100mM KSCN 10% Sucrose, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.68 Å R-free 0.261
2P9U Crystal structure of bovine Arp2/3 complex co-crystallized with AMP-PNP and calcium Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain C 1–372（372 aa）
Not recorded CA CALCIUM ION × 2 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；7.5% PEG3350 50mM HEPES 100mM KSCN 10% Sucrose, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.75 Å R-free 0.265
3DXK Structure of Bos Taurus Arp2/3 Complex with Bound Inhibitor CK0944636 Deposited 2008-07-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain C 1–372（372 aa）
Not recorded N23 N-[2-(2-methyl-1H-indol-3-yl)ethyl]thiophene-2-carboxamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；8% PEG 8000, 50 mM HEPES, pH 7.5, 100 mM KSCN, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.70 Å R-free 0.262
3DXM Structure of Bos taurus Arp2/3 Complex with Bound Inhibitor CK0993548 Deposited 2008-07-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain C 1–372（372 aa）
Not recorded N24 (2S)-2-(3-bromophenyl)-3-(5-chloro-2-hydroxyphenyl)-1,3-thiazolidin-4-one × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；8% PEG 8000, 50 mM HEPES, pH 7.5,100 mM KSCN, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.85 Å R-free 0.258
3RSE Structural and biochemical characterization of two binding sites for nucleation promoting factor WASp-VCA on Arp2/3 complex Deposited 2011-05-02 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain C 1–372（372 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；100 mM HEPES 7.5, 8% PEG 8000, 200 mM KCl, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.65 Å R-free 0.259
3UKR Crystal structure of Bos taurus Arp2/3 complex with bound inhibitor CK-666 Deposited 2011-11-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain C 1–372（372 aa）
Not recorded CKH 2-fluoro-N-[2-(2-methyl-1H-indol-3-yl)ethyl]benzamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；0.1M Hepes (pH 7.5), 0.2M KSCN, 16% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.48 Å R-free 0.261
3UKU Structure of Arp2/3 complex with bound inhibitor CK-869 Deposited 2011-11-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain C 1–372（372 aa）
Not recorded C69 (2S)-2-(3-bromophenyl)-3-(2,4-dimethoxyphenyl)-1,3-thiazolidin-4-one × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；278 K；8% PEG 8000, 50 mM HEPES, pH 7.5, 100 mM KSCN, VAPOR DIFFUSION, HANGING DROP, temperature 278K
Resolution 2.75 Å R-free 0.301
3ULE Structure of Bos taurus Arp2/3 complex with bound inhibitor CK-869 and ATP Deposited 2011-11-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain C 1–372（372 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 2 C69 (2S)-2-(3-bromophenyl)-3-(2,4-dimethoxyphenyl)-1,3-thiazolidin-4-one × 1 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；277 K；8% PEG 8000, 50 mM HEPES, pH 7.5,100 mM KSCN, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.50 Å R-free 0.274
4XEI Orthorhombic isomorph of bovine Arp2/3 complex Deposited 2014-12-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain C 1–372（372 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.4；277 K；Ethanol 10%, 1,4-butanediol 15%, 100 mM Tris 7.4
Resolution 3.87 Å R-free 0.329
4XF2 Tetragonal structure of Arp2/3 complex Deposited 2014-12-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain C 1–372（372 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；100 mM Tris 7.0, 15% ethanol
Resolution 5.00 Å R-free 0.303
4XF2 Tetragonal structure of Arp2/3 complex Deposited 2014-12-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain V 1–372（372 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；100 mM Tris 7.0, 15% ethanol
Resolution 5.00 Å R-free 0.303
6DEC Crystal structure of Bos taurus Arp2/3 complex binding with C-terminus of Homo sapiens SPIN90 Deposited 2018-05-11 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain C 1–372（372 aa）
Not recorded CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；50 mM HEPES, pH 7.5, 5% PEG3350, 50 mM L-Proline, 0.5 mM ATP, 0.5 mM calcium chloride, 1 mM DTT
Resolution 4.60 Å R-free 0.314
6DEC Crystal structure of Bos taurus Arp2/3 complex binding with C-terminus of Homo sapiens SPIN90 Deposited 2018-05-11 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain J 1–372（372 aa）
Not recorded CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；50 mM HEPES, pH 7.5, 5% PEG3350, 50 mM L-Proline, 0.5 mM ATP, 0.5 mM calcium chloride, 1 mM DTT
Resolution 4.60 Å R-free 0.314
7JPN Cryo-EM structure of Arpin-bound Arp2/3 complex Deposited 2020-08-09 Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain C 1–372（372 aa）
Not recorded MG MAGNESIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE；Grids were manually blotted for 3 seconds with Whatman 41 filter paper and manually plunged using a Leica EM CPC manual plunger.
Resolution 3.24 Å
7TPT Single-particle Cryo-EM structure of Arp2/3 complex at branched-actin junction. Deposited 2022-01-26 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 36 PDB declaration: 36-meric
Chain C 1–372（372 aa）
Not recorded MG MAGNESIUM ION × 16 ADP ADENOSINE-5'-DIPHOSPHATE × 16 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
9M5E Structure of SPIN90 dimer-Arp2/3 complex-nucleated actin filament (singlet complex) Deposited 2025-03-05 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric
Chain C 1–372（372 aa）
Not recorded MG MAGNESIUM ION × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.55 Å
9M64 Structure of SPIN90 dimer-Arp2/3 complexes-nucleated actin filaments (Doublet Complex) Deposited 2025-03-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain C 1–372（372 aa）
Chain c 1–372（372 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 12 MG MAGNESIUM ION × 10 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å

21 other PDB entries and 23 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name ARC1B_BOVIN
Isoform —
PDB entities 3
Chains and sequence ranges Author chain C； PDBConstruct 1–372； UniProt 1–372

Actin-related protein 2/3 complex subunit 2

OrganismNot specified

UniProt Q3MHR7

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count Chain D； UniProt 1–300 Not recorded Actin-related protein 3 × 1 （P61157） Actin-related protein 2 × 1 （A7MB62） Actin-related protein 2/3 complex subunit 1B × 1 （Q58CQ2） Actin-related protein 2/3 complex subunit 3 × 1 （Q3T035） Actin-related protein 2/3 complex subunit 4 × 1 （Q148J6） Actin-related protein 2/3 complex subunit 5 × 1 （Q3SYX9） Glia maturation factor gamma × 1 （Q9ERL7） CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 PG4 TETRAETHYLENE GLYCOL × 2 X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions Resolution 3.08 Å R-free 0.248

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4JD2 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1K8K Crystal Structure of Arp2/3 Complex Deposited 2001-10-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain D 1–300（300 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；PEG 8000, KSCN, Hepes at pH 7.5, VAPOR DIFFUSION, HANGING DROP at 298K
Resolution 2.00 Å R-free 0.251
2P9I Crystal Structure of bovine Arp2/3 Complex co-crystallized with ADP and crosslinked with gluteraldehyde Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain D 1–300（300 aa）
Not recorded CA CALCIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；7.5% PEG 3350 50mM HEPES 100mM KSCN 10% Sucrose, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.46 Å R-free 0.261
2P9K Crystal structure of bovine Arp2/3 complex co-crystallized with ATP and crosslinked with glutaraldehyde Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain D 1–300（300 aa）
Not recorded CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；278 K；7.5% PEG 3350, 50mM HEPES, 100mM KSCN, 10% Sucrose, VAPOR DIFFUSION, HANGING DROP, temperature 278K
Resolution 2.59 Å R-free 0.260
2P9L Crystal Structure of bovine Arp2/3 complex Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain D 1–300（300 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；7.5% PEG3350 50mM HEPES 100mM KSCN 10% Sucrose, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.65 Å R-free 0.266
2P9N Crystal Structure of bovine Arp2/3 complex co-crystallized with ADP Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain D 1–300（300 aa）
Not recorded CA CALCIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；6% PEG 8000 100mM HEPES 100mM KSCN, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.85 Å R-free 0.272
2P9P Crystal Structure of bovine Arp2/3 complex co-crystallized with ADP Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain D 1–300（300 aa）
Not recorded CA CALCIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；7.5% PEG 3350 50mM HEPES 100mM KSCN 10% Sucrose, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.90 Å R-free 0.288
2P9S Structure of bovine Arp2/3 complex co-crystallized with ATP/Mg2+ Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain D 1–300（300 aa）
Not recorded MG MAGNESIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；7.5% PEG3350 50mM HEPES 100mM KSCN 10% Sucrose, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.68 Å R-free 0.261
2P9U Crystal structure of bovine Arp2/3 complex co-crystallized with AMP-PNP and calcium Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain D 1–300（300 aa）
Not recorded CA CALCIUM ION × 2 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；7.5% PEG3350 50mM HEPES 100mM KSCN 10% Sucrose, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.75 Å R-free 0.265
3DXK Structure of Bos Taurus Arp2/3 Complex with Bound Inhibitor CK0944636 Deposited 2008-07-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain D 1–300（300 aa）
Not recorded N23 N-[2-(2-methyl-1H-indol-3-yl)ethyl]thiophene-2-carboxamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；8% PEG 8000, 50 mM HEPES, pH 7.5, 100 mM KSCN, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.70 Å R-free 0.262
3DXM Structure of Bos taurus Arp2/3 Complex with Bound Inhibitor CK0993548 Deposited 2008-07-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain D 1–300（300 aa）
Not recorded N24 (2S)-2-(3-bromophenyl)-3-(5-chloro-2-hydroxyphenyl)-1,3-thiazolidin-4-one × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；8% PEG 8000, 50 mM HEPES, pH 7.5,100 mM KSCN, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.85 Å R-free 0.258
3RSE Structural and biochemical characterization of two binding sites for nucleation promoting factor WASp-VCA on Arp2/3 complex Deposited 2011-05-02 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain D 1–300（300 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；100 mM HEPES 7.5, 8% PEG 8000, 200 mM KCl, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.65 Å R-free 0.259
3UKR Crystal structure of Bos taurus Arp2/3 complex with bound inhibitor CK-666 Deposited 2011-11-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain D 1–300（300 aa）
Not recorded CKH 2-fluoro-N-[2-(2-methyl-1H-indol-3-yl)ethyl]benzamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；0.1M Hepes (pH 7.5), 0.2M KSCN, 16% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.48 Å R-free 0.261
3UKU Structure of Arp2/3 complex with bound inhibitor CK-869 Deposited 2011-11-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain D 1–300（300 aa）
Not recorded C69 (2S)-2-(3-bromophenyl)-3-(2,4-dimethoxyphenyl)-1,3-thiazolidin-4-one × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；278 K；8% PEG 8000, 50 mM HEPES, pH 7.5, 100 mM KSCN, VAPOR DIFFUSION, HANGING DROP, temperature 278K
Resolution 2.75 Å R-free 0.301
3ULE Structure of Bos taurus Arp2/3 complex with bound inhibitor CK-869 and ATP Deposited 2011-11-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain D 1–300（300 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 2 C69 (2S)-2-(3-bromophenyl)-3-(2,4-dimethoxyphenyl)-1,3-thiazolidin-4-one × 1 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；277 K；8% PEG 8000, 50 mM HEPES, pH 7.5,100 mM KSCN, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.50 Å R-free 0.274
4XEI Orthorhombic isomorph of bovine Arp2/3 complex Deposited 2014-12-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain D 1–300（300 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.4；277 K；Ethanol 10%, 1,4-butanediol 15%, 100 mM Tris 7.4
Resolution 3.87 Å R-free 0.329
4XF2 Tetragonal structure of Arp2/3 complex Deposited 2014-12-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain D 1–300（300 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；100 mM Tris 7.0, 15% ethanol
Resolution 5.00 Å R-free 0.303
4XF2 Tetragonal structure of Arp2/3 complex Deposited 2014-12-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain W 1–300（300 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；100 mM Tris 7.0, 15% ethanol
Resolution 5.00 Å R-free 0.303
6DEC Crystal structure of Bos taurus Arp2/3 complex binding with C-terminus of Homo sapiens SPIN90 Deposited 2018-05-11 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain D 1–300（300 aa）
Not recorded CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；50 mM HEPES, pH 7.5, 5% PEG3350, 50 mM L-Proline, 0.5 mM ATP, 0.5 mM calcium chloride, 1 mM DTT
Resolution 4.60 Å R-free 0.314
6DEC Crystal structure of Bos taurus Arp2/3 complex binding with C-terminus of Homo sapiens SPIN90 Deposited 2018-05-11 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain K 1–300（300 aa）
Not recorded CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；50 mM HEPES, pH 7.5, 5% PEG3350, 50 mM L-Proline, 0.5 mM ATP, 0.5 mM calcium chloride, 1 mM DTT
Resolution 4.60 Å R-free 0.314
7JPN Cryo-EM structure of Arpin-bound Arp2/3 complex Deposited 2020-08-09 Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain D 1–300（300 aa）
Not recorded MG MAGNESIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE；Grids were manually blotted for 3 seconds with Whatman 41 filter paper and manually plunged using a Leica EM CPC manual plunger.
Resolution 3.24 Å
7T5Q Cryo-EM Structure of a Transition State of Arp2/3 Complex Activation Deposited 2021-12-13 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 10 PDB declaration: decameric
Chain D 1–300（300 aa）
Not recorded MG MAGNESIUM ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE；Grids were manually blotted for 3 seconds with Whatman 41 filter paper and manually plunged using a Leica EM CPC manual plunger.
Resolution 3.40 Å
7TPT Single-particle Cryo-EM structure of Arp2/3 complex at branched-actin junction. Deposited 2022-01-26 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 36 PDB declaration: 36-meric
Chain D 1–300（300 aa）
Not recorded MG MAGNESIUM ION × 16 ADP ADENOSINE-5'-DIPHOSPHATE × 16 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8TAH Cryo-EM structure of Cortactin-bound to Arp2/3 complex Deposited 2023-06-27 Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain D 1–300（300 aa）
Not recorded MG MAGNESIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7；10 mM imidazole pH 7.0, 50 mM KCl, 2 mM MgCl2, 1 mM EGTA
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.89 Å
9DLX Bovine Arp2/3 complex with N-WASP CA bound to Arp3 and Arp2-ArpC1 Deposited 2024-09-11 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric
Chain D 1–285（285 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.91 Å
9DLZ Bovine Arp2/3 complex with N-WASP CA bound to Arp3 Deposited 2024-09-11 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric
Chain D 1–285（285 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
9EAM Coronin-7 CA bound to Arp2/3 complex Deposited 2024-11-11 Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain D 1–300（300 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.97 Å
9M5E Structure of SPIN90 dimer-Arp2/3 complex-nucleated actin filament (singlet complex) Deposited 2025-03-05 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric
Chain D 1–300（300 aa）
Not recorded MG MAGNESIUM ION × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.55 Å
9M64 Structure of SPIN90 dimer-Arp2/3 complexes-nucleated actin filaments (Doublet Complex) Deposited 2025-03-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain D 1–300（300 aa）
Chain d 1–300（300 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 12 MG MAGNESIUM ION × 10 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å

26 other PDB entries and 28 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name ARPC2_BOVIN
Isoform —
PDB entities 4
Chains and sequence ranges Author chain D； PDBConstruct 1–300； UniProt 1–300

Actin-related protein 2/3 complex subunit 3

OrganismNot specified

UniProt Q3T035

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count Chain E； UniProt 1–178 Not recorded Actin-related protein 3 × 1 （P61157） Actin-related protein 2 × 1 （A7MB62） Actin-related protein 2/3 complex subunit 1B × 1 （Q58CQ2） Actin-related protein 2/3 complex subunit 2 × 1 （Q3MHR7） Actin-related protein 2/3 complex subunit 4 × 1 （Q148J6） Actin-related protein 2/3 complex subunit 5 × 1 （Q3SYX9） Glia maturation factor gamma × 1 （Q9ERL7） CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 PG4 TETRAETHYLENE GLYCOL × 2 X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions Resolution 3.08 Å R-free 0.248

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4JD2 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1K8K Crystal Structure of Arp2/3 Complex Deposited 2001-10-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain E 1–178（178 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；PEG 8000, KSCN, Hepes at pH 7.5, VAPOR DIFFUSION, HANGING DROP at 298K
Resolution 2.00 Å R-free 0.251
2P9I Crystal Structure of bovine Arp2/3 Complex co-crystallized with ADP and crosslinked with gluteraldehyde Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain E 1–178（178 aa）
Not recorded CA CALCIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；7.5% PEG 3350 50mM HEPES 100mM KSCN 10% Sucrose, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.46 Å R-free 0.261
2P9K Crystal structure of bovine Arp2/3 complex co-crystallized with ATP and crosslinked with glutaraldehyde Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain E 1–178（178 aa）
Not recorded CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；278 K；7.5% PEG 3350, 50mM HEPES, 100mM KSCN, 10% Sucrose, VAPOR DIFFUSION, HANGING DROP, temperature 278K
Resolution 2.59 Å R-free 0.260
2P9L Crystal Structure of bovine Arp2/3 complex Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain E 1–178（178 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；7.5% PEG3350 50mM HEPES 100mM KSCN 10% Sucrose, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.65 Å R-free 0.266
2P9N Crystal Structure of bovine Arp2/3 complex co-crystallized with ADP Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain E 1–178（178 aa）
Not recorded CA CALCIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；6% PEG 8000 100mM HEPES 100mM KSCN, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.85 Å R-free 0.272
2P9P Crystal Structure of bovine Arp2/3 complex co-crystallized with ADP Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain E 1–178（178 aa）
Not recorded CA CALCIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；7.5% PEG 3350 50mM HEPES 100mM KSCN 10% Sucrose, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.90 Å R-free 0.288
2P9S Structure of bovine Arp2/3 complex co-crystallized with ATP/Mg2+ Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain E 1–178（178 aa）
Not recorded MG MAGNESIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；7.5% PEG3350 50mM HEPES 100mM KSCN 10% Sucrose, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.68 Å R-free 0.261
2P9U Crystal structure of bovine Arp2/3 complex co-crystallized with AMP-PNP and calcium Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain E 1–178（178 aa）
Not recorded CA CALCIUM ION × 2 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；7.5% PEG3350 50mM HEPES 100mM KSCN 10% Sucrose, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.75 Å R-free 0.265
3DXK Structure of Bos Taurus Arp2/3 Complex with Bound Inhibitor CK0944636 Deposited 2008-07-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain E 1–178（178 aa）
Not recorded N23 N-[2-(2-methyl-1H-indol-3-yl)ethyl]thiophene-2-carboxamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；8% PEG 8000, 50 mM HEPES, pH 7.5, 100 mM KSCN, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.70 Å R-free 0.262
3DXM Structure of Bos taurus Arp2/3 Complex with Bound Inhibitor CK0993548 Deposited 2008-07-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain E 1–178（178 aa）
Not recorded N24 (2S)-2-(3-bromophenyl)-3-(5-chloro-2-hydroxyphenyl)-1,3-thiazolidin-4-one × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；8% PEG 8000, 50 mM HEPES, pH 7.5,100 mM KSCN, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.85 Å R-free 0.258
3RSE Structural and biochemical characterization of two binding sites for nucleation promoting factor WASp-VCA on Arp2/3 complex Deposited 2011-05-02 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain E 1–178（178 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；100 mM HEPES 7.5, 8% PEG 8000, 200 mM KCl, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.65 Å R-free 0.259
3UKR Crystal structure of Bos taurus Arp2/3 complex with bound inhibitor CK-666 Deposited 2011-11-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain E 1–178（178 aa）
Not recorded CKH 2-fluoro-N-[2-(2-methyl-1H-indol-3-yl)ethyl]benzamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；0.1M Hepes (pH 7.5), 0.2M KSCN, 16% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.48 Å R-free 0.261
3UKU Structure of Arp2/3 complex with bound inhibitor CK-869 Deposited 2011-11-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain E 1–178（178 aa）
Not recorded C69 (2S)-2-(3-bromophenyl)-3-(2,4-dimethoxyphenyl)-1,3-thiazolidin-4-one × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；278 K；8% PEG 8000, 50 mM HEPES, pH 7.5, 100 mM KSCN, VAPOR DIFFUSION, HANGING DROP, temperature 278K
Resolution 2.75 Å R-free 0.301
3ULE Structure of Bos taurus Arp2/3 complex with bound inhibitor CK-869 and ATP Deposited 2011-11-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain E 1–178（178 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 2 C69 (2S)-2-(3-bromophenyl)-3-(2,4-dimethoxyphenyl)-1,3-thiazolidin-4-one × 1 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；277 K；8% PEG 8000, 50 mM HEPES, pH 7.5,100 mM KSCN, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.50 Å R-free 0.274
4XEI Orthorhombic isomorph of bovine Arp2/3 complex Deposited 2014-12-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain E 1–178（178 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.4；277 K；Ethanol 10%, 1,4-butanediol 15%, 100 mM Tris 7.4
Resolution 3.87 Å R-free 0.329
4XF2 Tetragonal structure of Arp2/3 complex Deposited 2014-12-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain E 1–178（178 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；100 mM Tris 7.0, 15% ethanol
Resolution 5.00 Å R-free 0.303
4XF2 Tetragonal structure of Arp2/3 complex Deposited 2014-12-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain X 1–178（178 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；100 mM Tris 7.0, 15% ethanol
Resolution 5.00 Å R-free 0.303
6DEC Crystal structure of Bos taurus Arp2/3 complex binding with C-terminus of Homo sapiens SPIN90 Deposited 2018-05-11 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain E 1–178（178 aa）
Not recorded CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；50 mM HEPES, pH 7.5, 5% PEG3350, 50 mM L-Proline, 0.5 mM ATP, 0.5 mM calcium chloride, 1 mM DTT
Resolution 4.60 Å R-free 0.314
6DEC Crystal structure of Bos taurus Arp2/3 complex binding with C-terminus of Homo sapiens SPIN90 Deposited 2018-05-11 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain L 1–178（178 aa）
Not recorded CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；50 mM HEPES, pH 7.5, 5% PEG3350, 50 mM L-Proline, 0.5 mM ATP, 0.5 mM calcium chloride, 1 mM DTT
Resolution 4.60 Å R-free 0.314
7JPN Cryo-EM structure of Arpin-bound Arp2/3 complex Deposited 2020-08-09 Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain E 1–178（178 aa）
Not recorded MG MAGNESIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE；Grids were manually blotted for 3 seconds with Whatman 41 filter paper and manually plunged using a Leica EM CPC manual plunger.
Resolution 3.24 Å
7T5Q Cryo-EM Structure of a Transition State of Arp2/3 Complex Activation Deposited 2021-12-13 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 10 PDB declaration: decameric
Chain E 1–178（178 aa）
Not recorded MG MAGNESIUM ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE；Grids were manually blotted for 3 seconds with Whatman 41 filter paper and manually plunged using a Leica EM CPC manual plunger.
Resolution 3.40 Å
7TPT Single-particle Cryo-EM structure of Arp2/3 complex at branched-actin junction. Deposited 2022-01-26 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 36 PDB declaration: 36-meric
Chain E 1–178（178 aa）
Not recorded MG MAGNESIUM ION × 16 ADP ADENOSINE-5'-DIPHOSPHATE × 16 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8TAH Cryo-EM structure of Cortactin-bound to Arp2/3 complex Deposited 2023-06-27 Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain E 1–178（178 aa）
Not recorded MG MAGNESIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7；10 mM imidazole pH 7.0, 50 mM KCl, 2 mM MgCl2, 1 mM EGTA
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.89 Å
9DLX Bovine Arp2/3 complex with N-WASP CA bound to Arp3 and Arp2-ArpC1 Deposited 2024-09-11 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric
Chain E 2–176（175 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.91 Å
9DLZ Bovine Arp2/3 complex with N-WASP CA bound to Arp3 Deposited 2024-09-11 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric
Chain E 2–176（175 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
9EAM Coronin-7 CA bound to Arp2/3 complex Deposited 2024-11-11 Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain E 1–178（178 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.97 Å
9M5E Structure of SPIN90 dimer-Arp2/3 complex-nucleated actin filament (singlet complex) Deposited 2025-03-05 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric
Chain E 1–178（178 aa）
Not recorded MG MAGNESIUM ION × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.55 Å
9M64 Structure of SPIN90 dimer-Arp2/3 complexes-nucleated actin filaments (Doublet Complex) Deposited 2025-03-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain E 1–178（178 aa）
Chain e 1–178（178 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 12 MG MAGNESIUM ION × 10 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å

26 other PDB entries and 28 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name ARPC3_BOVIN
Isoform —
PDB entities 5
Chains and sequence ranges Author chain E； PDBConstruct 1–178； UniProt 1–178

Actin-related protein 2/3 complex subunit 4

OrganismNot specified

UniProt Q148J6

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count Chain F； UniProt 1–168 Not recorded Actin-related protein 3 × 1 （P61157） Actin-related protein 2 × 1 （A7MB62） Actin-related protein 2/3 complex subunit 1B × 1 （Q58CQ2） Actin-related protein 2/3 complex subunit 2 × 1 （Q3MHR7） Actin-related protein 2/3 complex subunit 3 × 1 （Q3T035） Actin-related protein 2/3 complex subunit 5 × 1 （Q3SYX9） Glia maturation factor gamma × 1 （Q9ERL7） CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 PG4 TETRAETHYLENE GLYCOL × 2 X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions Resolution 3.08 Å R-free 0.248

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4JD2 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1K8K Crystal Structure of Arp2/3 Complex Deposited 2001-10-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain F 1–168（168 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；PEG 8000, KSCN, Hepes at pH 7.5, VAPOR DIFFUSION, HANGING DROP at 298K
Resolution 2.00 Å R-free 0.251
2P9I Crystal Structure of bovine Arp2/3 Complex co-crystallized with ADP and crosslinked with gluteraldehyde Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain F 1–168（168 aa）
Not recorded CA CALCIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；7.5% PEG 3350 50mM HEPES 100mM KSCN 10% Sucrose, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.46 Å R-free 0.261
2P9K Crystal structure of bovine Arp2/3 complex co-crystallized with ATP and crosslinked with glutaraldehyde Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain F 1–168（168 aa）
Not recorded CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；278 K；7.5% PEG 3350, 50mM HEPES, 100mM KSCN, 10% Sucrose, VAPOR DIFFUSION, HANGING DROP, temperature 278K
Resolution 2.59 Å R-free 0.260
2P9L Crystal Structure of bovine Arp2/3 complex Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain F 1–168（168 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；7.5% PEG3350 50mM HEPES 100mM KSCN 10% Sucrose, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.65 Å R-free 0.266
2P9N Crystal Structure of bovine Arp2/3 complex co-crystallized with ADP Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain F 1–168（168 aa）
Not recorded CA CALCIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；6% PEG 8000 100mM HEPES 100mM KSCN, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.85 Å R-free 0.272
2P9P Crystal Structure of bovine Arp2/3 complex co-crystallized with ADP Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain F 1–168（168 aa）
Not recorded CA CALCIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；7.5% PEG 3350 50mM HEPES 100mM KSCN 10% Sucrose, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.90 Å R-free 0.288
2P9S Structure of bovine Arp2/3 complex co-crystallized with ATP/Mg2+ Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain F 1–168（168 aa）
Not recorded MG MAGNESIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；7.5% PEG3350 50mM HEPES 100mM KSCN 10% Sucrose, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.68 Å R-free 0.261
2P9U Crystal structure of bovine Arp2/3 complex co-crystallized with AMP-PNP and calcium Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain F 1–168（168 aa）
Not recorded CA CALCIUM ION × 2 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；7.5% PEG3350 50mM HEPES 100mM KSCN 10% Sucrose, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.75 Å R-free 0.265
3DXK Structure of Bos Taurus Arp2/3 Complex with Bound Inhibitor CK0944636 Deposited 2008-07-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain F 1–168（168 aa）
Not recorded N23 N-[2-(2-methyl-1H-indol-3-yl)ethyl]thiophene-2-carboxamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；8% PEG 8000, 50 mM HEPES, pH 7.5, 100 mM KSCN, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.70 Å R-free 0.262
3DXM Structure of Bos taurus Arp2/3 Complex with Bound Inhibitor CK0993548 Deposited 2008-07-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain F 1–168（168 aa）
Not recorded N24 (2S)-2-(3-bromophenyl)-3-(5-chloro-2-hydroxyphenyl)-1,3-thiazolidin-4-one × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；8% PEG 8000, 50 mM HEPES, pH 7.5,100 mM KSCN, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.85 Å R-free 0.258
3RSE Structural and biochemical characterization of two binding sites for nucleation promoting factor WASp-VCA on Arp2/3 complex Deposited 2011-05-02 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain F 1–168（168 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；100 mM HEPES 7.5, 8% PEG 8000, 200 mM KCl, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.65 Å R-free 0.259
3UKR Crystal structure of Bos taurus Arp2/3 complex with bound inhibitor CK-666 Deposited 2011-11-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain F 1–168（168 aa）
Not recorded CKH 2-fluoro-N-[2-(2-methyl-1H-indol-3-yl)ethyl]benzamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；0.1M Hepes (pH 7.5), 0.2M KSCN, 16% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.48 Å R-free 0.261
3UKU Structure of Arp2/3 complex with bound inhibitor CK-869 Deposited 2011-11-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain F 1–168（168 aa）
Not recorded C69 (2S)-2-(3-bromophenyl)-3-(2,4-dimethoxyphenyl)-1,3-thiazolidin-4-one × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；278 K；8% PEG 8000, 50 mM HEPES, pH 7.5, 100 mM KSCN, VAPOR DIFFUSION, HANGING DROP, temperature 278K
Resolution 2.75 Å R-free 0.301
3ULE Structure of Bos taurus Arp2/3 complex with bound inhibitor CK-869 and ATP Deposited 2011-11-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain F 1–168（168 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 2 C69 (2S)-2-(3-bromophenyl)-3-(2,4-dimethoxyphenyl)-1,3-thiazolidin-4-one × 1 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；277 K；8% PEG 8000, 50 mM HEPES, pH 7.5,100 mM KSCN, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.50 Å R-free 0.274
4XEI Orthorhombic isomorph of bovine Arp2/3 complex Deposited 2014-12-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain F 1–168（168 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.4；277 K；Ethanol 10%, 1,4-butanediol 15%, 100 mM Tris 7.4
Resolution 3.87 Å R-free 0.329
4XF2 Tetragonal structure of Arp2/3 complex Deposited 2014-12-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain F 1–168（168 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；100 mM Tris 7.0, 15% ethanol
Resolution 5.00 Å R-free 0.303
4XF2 Tetragonal structure of Arp2/3 complex Deposited 2014-12-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain Y 1–168（168 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；100 mM Tris 7.0, 15% ethanol
Resolution 5.00 Å R-free 0.303
6DEC Crystal structure of Bos taurus Arp2/3 complex binding with C-terminus of Homo sapiens SPIN90 Deposited 2018-05-11 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain F 1–168（168 aa）
Not recorded CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；50 mM HEPES, pH 7.5, 5% PEG3350, 50 mM L-Proline, 0.5 mM ATP, 0.5 mM calcium chloride, 1 mM DTT
Resolution 4.60 Å R-free 0.314
6DEC Crystal structure of Bos taurus Arp2/3 complex binding with C-terminus of Homo sapiens SPIN90 Deposited 2018-05-11 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain N 1–168（168 aa）
Not recorded CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；50 mM HEPES, pH 7.5, 5% PEG3350, 50 mM L-Proline, 0.5 mM ATP, 0.5 mM calcium chloride, 1 mM DTT
Resolution 4.60 Å R-free 0.314
7JPN Cryo-EM structure of Arpin-bound Arp2/3 complex Deposited 2020-08-09 Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain F 1–168（168 aa）
Not recorded MG MAGNESIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE；Grids were manually blotted for 3 seconds with Whatman 41 filter paper and manually plunged using a Leica EM CPC manual plunger.
Resolution 3.24 Å
7T5Q Cryo-EM Structure of a Transition State of Arp2/3 Complex Activation Deposited 2021-12-13 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 10 PDB declaration: decameric
Chain F 1–168（168 aa）
Not recorded MG MAGNESIUM ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE；Grids were manually blotted for 3 seconds with Whatman 41 filter paper and manually plunged using a Leica EM CPC manual plunger.
Resolution 3.40 Å
7TPT Single-particle Cryo-EM structure of Arp2/3 complex at branched-actin junction. Deposited 2022-01-26 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 36 PDB declaration: 36-meric
Chain F 1–168（168 aa）
Not recorded MG MAGNESIUM ION × 16 ADP ADENOSINE-5'-DIPHOSPHATE × 16 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8TAH Cryo-EM structure of Cortactin-bound to Arp2/3 complex Deposited 2023-06-27 Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain F 1–168（168 aa）
Not recorded MG MAGNESIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7；10 mM imidazole pH 7.0, 50 mM KCl, 2 mM MgCl2, 1 mM EGTA
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.89 Å
9DLX Bovine Arp2/3 complex with N-WASP CA bound to Arp3 and Arp2-ArpC1 Deposited 2024-09-11 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric
Chain F 2–168（167 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.91 Å
9DLZ Bovine Arp2/3 complex with N-WASP CA bound to Arp3 Deposited 2024-09-11 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric
Chain F 2–168（167 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
9EAM Coronin-7 CA bound to Arp2/3 complex Deposited 2024-11-11 Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain F 1–168（168 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.97 Å
9M5E Structure of SPIN90 dimer-Arp2/3 complex-nucleated actin filament (singlet complex) Deposited 2025-03-05 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric
Chain F 1–168（168 aa）
Not recorded MG MAGNESIUM ION × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.55 Å
9M64 Structure of SPIN90 dimer-Arp2/3 complexes-nucleated actin filaments (Doublet Complex) Deposited 2025-03-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain F 1–168（168 aa）
Chain f 1–168（168 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 12 MG MAGNESIUM ION × 10 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å

26 other PDB entries and 28 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name ARPC4_BOVIN
Isoform —
PDB entities 6
Chains and sequence ranges Author chain F； PDBConstruct 1–168； UniProt 1–168

Actin-related protein 2/3 complex subunit 5

OrganismNot specified

UniProt Q3SYX9

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count Chain G； UniProt 1–151 Not recorded Actin-related protein 3 × 1 （P61157） Actin-related protein 2 × 1 （A7MB62） Actin-related protein 2/3 complex subunit 1B × 1 （Q58CQ2） Actin-related protein 2/3 complex subunit 2 × 1 （Q3MHR7） Actin-related protein 2/3 complex subunit 3 × 1 （Q3T035） Actin-related protein 2/3 complex subunit 4 × 1 （Q148J6） Glia maturation factor gamma × 1 （Q9ERL7） CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 PG4 TETRAETHYLENE GLYCOL × 2 X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions Resolution 3.08 Å R-free 0.248

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4JD2 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1K8K Crystal Structure of Arp2/3 Complex Deposited 2001-10-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain G 1–151（151 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；PEG 8000, KSCN, Hepes at pH 7.5, VAPOR DIFFUSION, HANGING DROP at 298K
Resolution 2.00 Å R-free 0.251
2P9I Crystal Structure of bovine Arp2/3 Complex co-crystallized with ADP and crosslinked with gluteraldehyde Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain G 1–151（151 aa）
Not recorded CA CALCIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；7.5% PEG 3350 50mM HEPES 100mM KSCN 10% Sucrose, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.46 Å R-free 0.261
2P9K Crystal structure of bovine Arp2/3 complex co-crystallized with ATP and crosslinked with glutaraldehyde Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain G 1–151（151 aa）
Not recorded CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；278 K；7.5% PEG 3350, 50mM HEPES, 100mM KSCN, 10% Sucrose, VAPOR DIFFUSION, HANGING DROP, temperature 278K
Resolution 2.59 Å R-free 0.260
2P9L Crystal Structure of bovine Arp2/3 complex Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain G 1–151（151 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；7.5% PEG3350 50mM HEPES 100mM KSCN 10% Sucrose, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.65 Å R-free 0.266
2P9N Crystal Structure of bovine Arp2/3 complex co-crystallized with ADP Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain G 1–151（151 aa）
Not recorded CA CALCIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；6% PEG 8000 100mM HEPES 100mM KSCN, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.85 Å R-free 0.272
2P9P Crystal Structure of bovine Arp2/3 complex co-crystallized with ADP Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain G 1–151（151 aa）
Not recorded CA CALCIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；7.5% PEG 3350 50mM HEPES 100mM KSCN 10% Sucrose, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.90 Å R-free 0.288
2P9S Structure of bovine Arp2/3 complex co-crystallized with ATP/Mg2+ Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain G 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；7.5% PEG3350 50mM HEPES 100mM KSCN 10% Sucrose, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.68 Å R-free 0.261
2P9U Crystal structure of bovine Arp2/3 complex co-crystallized with AMP-PNP and calcium Deposited 2007-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain G 1–151（151 aa）
Not recorded CA CALCIUM ION × 2 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；7.5% PEG3350 50mM HEPES 100mM KSCN 10% Sucrose, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.75 Å R-free 0.265
3DXK Structure of Bos Taurus Arp2/3 Complex with Bound Inhibitor CK0944636 Deposited 2008-07-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain G 1–151（151 aa）
Not recorded N23 N-[2-(2-methyl-1H-indol-3-yl)ethyl]thiophene-2-carboxamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；8% PEG 8000, 50 mM HEPES, pH 7.5, 100 mM KSCN, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.70 Å R-free 0.262
3DXM Structure of Bos taurus Arp2/3 Complex with Bound Inhibitor CK0993548 Deposited 2008-07-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain G 1–151（151 aa）
Not recorded N24 (2S)-2-(3-bromophenyl)-3-(5-chloro-2-hydroxyphenyl)-1,3-thiazolidin-4-one × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；8% PEG 8000, 50 mM HEPES, pH 7.5,100 mM KSCN, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.85 Å R-free 0.258
3RSE Structural and biochemical characterization of two binding sites for nucleation promoting factor WASp-VCA on Arp2/3 complex Deposited 2011-05-02 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain G 1–151（151 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；100 mM HEPES 7.5, 8% PEG 8000, 200 mM KCl, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.65 Å R-free 0.259
3UKR Crystal structure of Bos taurus Arp2/3 complex with bound inhibitor CK-666 Deposited 2011-11-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain G 1–151（151 aa）
Not recorded CKH 2-fluoro-N-[2-(2-methyl-1H-indol-3-yl)ethyl]benzamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；0.1M Hepes (pH 7.5), 0.2M KSCN, 16% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.48 Å R-free 0.261
3UKU Structure of Arp2/3 complex with bound inhibitor CK-869 Deposited 2011-11-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain G 1–151（151 aa）
Not recorded C69 (2S)-2-(3-bromophenyl)-3-(2,4-dimethoxyphenyl)-1,3-thiazolidin-4-one × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；278 K；8% PEG 8000, 50 mM HEPES, pH 7.5, 100 mM KSCN, VAPOR DIFFUSION, HANGING DROP, temperature 278K
Resolution 2.75 Å R-free 0.301
3ULE Structure of Bos taurus Arp2/3 complex with bound inhibitor CK-869 and ATP Deposited 2011-11-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain G 1–151（151 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 2 C69 (2S)-2-(3-bromophenyl)-3-(2,4-dimethoxyphenyl)-1,3-thiazolidin-4-one × 1 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；277 K；8% PEG 8000, 50 mM HEPES, pH 7.5,100 mM KSCN, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.50 Å R-free 0.274
4XEI Orthorhombic isomorph of bovine Arp2/3 complex Deposited 2014-12-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain G 1–151（151 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.4；277 K；Ethanol 10%, 1,4-butanediol 15%, 100 mM Tris 7.4
Resolution 3.87 Å R-free 0.329
4XF2 Tetragonal structure of Arp2/3 complex Deposited 2014-12-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain G 1–151（151 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；100 mM Tris 7.0, 15% ethanol
Resolution 5.00 Å R-free 0.303
4XF2 Tetragonal structure of Arp2/3 complex Deposited 2014-12-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain Z 1–151（151 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；100 mM Tris 7.0, 15% ethanol
Resolution 5.00 Å R-free 0.303
6DEC Crystal structure of Bos taurus Arp2/3 complex binding with C-terminus of Homo sapiens SPIN90 Deposited 2018-05-11 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain G 1–151（151 aa）
Not recorded CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；50 mM HEPES, pH 7.5, 5% PEG3350, 50 mM L-Proline, 0.5 mM ATP, 0.5 mM calcium chloride, 1 mM DTT
Resolution 4.60 Å R-free 0.314
6DEC Crystal structure of Bos taurus Arp2/3 complex binding with C-terminus of Homo sapiens SPIN90 Deposited 2018-05-11 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain O 1–151（151 aa）
Not recorded CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；50 mM HEPES, pH 7.5, 5% PEG3350, 50 mM L-Proline, 0.5 mM ATP, 0.5 mM calcium chloride, 1 mM DTT
Resolution 4.60 Å R-free 0.314
7T5Q Cryo-EM Structure of a Transition State of Arp2/3 Complex Activation Deposited 2021-12-13 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 10 PDB declaration: decameric
Chain G 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE；Grids were manually blotted for 3 seconds with Whatman 41 filter paper and manually plunged using a Leica EM CPC manual plunger.
Resolution 3.40 Å
7TPT Single-particle Cryo-EM structure of Arp2/3 complex at branched-actin junction. Deposited 2022-01-26 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 36 PDB declaration: 36-meric
Chain G 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 16 ADP ADENOSINE-5'-DIPHOSPHATE × 16 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
9DLX Bovine Arp2/3 complex with N-WASP CA bound to Arp3 and Arp2-ArpC1 Deposited 2024-09-11 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric
Chain G 1–151（151 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.91 Å
9DLZ Bovine Arp2/3 complex with N-WASP CA bound to Arp3 Deposited 2024-09-11 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric
Chain G 7–151（145 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
9EAM Coronin-7 CA bound to Arp2/3 complex Deposited 2024-11-11 Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain G 1–151（151 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.97 Å
9M5E Structure of SPIN90 dimer-Arp2/3 complex-nucleated actin filament (singlet complex) Deposited 2025-03-05 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric
Chain G 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.55 Å
9M64 Structure of SPIN90 dimer-Arp2/3 complexes-nucleated actin filaments (Doublet Complex) Deposited 2025-03-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain G 1–151（151 aa）
Chain g 1–151（151 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 12 MG MAGNESIUM ION × 10 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å

24 other PDB entries and 26 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name ARPC5_BOVIN
Isoform —
PDB entities 7
Chains and sequence ranges Author chain G； PDBConstruct 1–151； UniProt 1–151

Glia maturation factor gamma

OrganismNot specified

UniProt Q9ERL7

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count Chain H； UniProt 1–142 Not recorded Actin-related protein 3 × 1 （P61157） Actin-related protein 2 × 1 （A7MB62） Actin-related protein 2/3 complex subunit 1B × 1 （Q58CQ2） Actin-related protein 2/3 complex subunit 2 × 1 （Q3MHR7） Actin-related protein 2/3 complex subunit 3 × 1 （Q3T035） Actin-related protein 2/3 complex subunit 4 × 1 （Q148J6） Actin-related protein 2/3 complex subunit 5 × 1 （Q3SYX9） CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 PG4 TETRAETHYLENE GLYCOL × 2 X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions Resolution 3.08 Å R-free 0.248

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4JD2 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1VKK Crystal structure of Glia maturation factor-gamma (GMFG) from Mus musculus at 1.50 A resolution Deposited 2004-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–142（142 aa）
Not recorded PO4 PHOSPHATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION,SITTING DROP,NANODROP；pH 8.5；277 K；30% PEG-4000, 0.1M Tris hydrochloride pH 8.5,0.2M lithium sulfate monohydrate, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K
Resolution 1.35 Å R-free 0.194
1WFS Solution Structure of Glia Maturation Factor-gamma from Mus Musculus Deposited 2004-05-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 2–139（138 aa） Fragment：COFILIN-ADF-domain
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6；298 K；Ionic strength (raw mmCIF value) 120mM；Pressure ambient
NMR sample composition 1.21mM cofilin U-15N,13C; 20mM phosphate buffer Na; 100mM NaCl; 0.02% NaN3; 10% D2O | 90% H2O/10% D2O
Resolution not provided

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name GMFG_MOUSE
Isoform —
PDB entities 8
Chains and sequence ranges Author chain H； PDBConstruct 1–142； UniProt 1–142

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 4jd2

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 4jd2
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2. Structure Basics 2. Structure Basics

Entry ID entry_id4jd2
Deposition date deposition_date2013-02-22
Structure title titleCrystal structure of Bos taurus Arp2/3 complex binding with Mus musculus GMF
Keywords keywordsactin filament polymerization and branching, STRUCTURAL PROTEIN; STRUCTURAL PROTEIN
Experimental Method methodX-RAY DIFFRACTION
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier45.11 Å
Radius of gyration Rg (electron density) rg_electron44.70 Å
Forward intensity I(0) i0746548000.00
Molecular weight molecular_weight227640.0 kDa
Excluded volume excluded_volume285520 ų
Envelope volume envelope_volume381160 ų
Hydration-shell volume shell_volume70116 ų
Envelope diameter envelope_diameter150.4 Å
Shell Rg shell_rg49.95 Å
Envelope Rg envelope_rg44.43 Å
Shape Rg shape_rg44.72 Å
Total Rg total_rg44.87 Å
Total atoms total_atoms16016
Residues n_residues2013
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax147.7 Å
Rg (real space) rg_real45.02 Å
Rg uncertainty (real space) rg_real_error1.32 Å
I(0) (real space) i0_real7.4650e+08
I(0) uncertainty (real space) i0_real_error1.3350e+07
Rg (reciprocal space) rg_reciprocal45.11 Å
I(0) (reciprocal space) i0_reciprocal746600000.0000
Solution quality estimate total_estimate0.8982
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks1
Primary peak position r_peak_primary54.9 Å
Skewness Skewness skewness0.191
Kurtosis Kurtosis kurtosis-0.634
Angular range angular_range— – 0.1750 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha101600000.0000
Real-space data points n_real_points36
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.926; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.990; Smooth: 0.903
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (11)

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7. Fold Classification (SCOP + CATH) 22 domains

SCOP 2.08 (9 domains)

Domain ID domain_idd4jd2a1
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.55 — Ribonuclease H-like motif
Superfamily Superfamily superfamilyc.55.1 — Actin-like ATPase domain
Family Family familyc.55.1.0 — automated matches
Domain ID domain_idd4jd2a2
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.55 — Ribonuclease H-like motif
Superfamily Superfamily superfamilyc.55.1 — Actin-like ATPase domain
Family Family familyc.55.1.1 — Actin/HSP70
Domain ID domain_idd4jd2c_
Class classb — All beta proteins
Fold Fold foldb.69 — 7-bladed beta-propeller
Superfamily Superfamily superfamilyb.69.4 — WD40 repeat-like
Family Family familyb.69.4.1 — WD40-repeat
Domain ID domain_idd4jd2d1
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.198 — Secretion chaperone-like
Superfamily Superfamily superfamilyd.198.2 — Arp2/3 complex subunits
Family Family familyd.198.2.1 — Arp2/3 complex subunits
Domain ID domain_idd4jd2d2
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.198 — Secretion chaperone-like
Superfamily Superfamily superfamilyd.198.2 — Arp2/3 complex subunits
Family Family familyd.198.2.1 — Arp2/3 complex subunits
Domain ID domain_idd4jd2e_
Class classa — All alpha proteins
Fold Fold folda.148 — Arp2/3 complex 21 kDa subunit ARPC3
Superfamily Superfamily superfamilya.148.1 — Arp2/3 complex 21 kDa subunit ARPC3
Family Family familya.148.1.1 — Arp2/3 complex 21 kDa subunit ARPC3
Domain ID domain_idd4jd2f_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.198 — Secretion chaperone-like
Superfamily Superfamily superfamilyd.198.2 — Arp2/3 complex subunits
Family Family familyd.198.2.1 — Arp2/3 complex subunits
Domain ID domain_idd4jd2g_
Class classa — All alpha proteins
Fold Fold folda.118 — alpha-alpha superhelix
Superfamily Superfamily superfamilya.118.13 — Arp2/3 complex 16 kDa subunit ARPC5
Family Family familya.118.13.1 — Arp2/3 complex 16 kDa subunit ARPC5
Domain ID domain_idd4jd2h_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.109 — Gelsolin-like
Superfamily Superfamily superfamilyd.109.1 — Actin depolymerizing proteins
Family Family familyd.109.1.2 — Cofilin-like

CATH v4.4 (13 domains)

Domain ID domain_id4jd2A01
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology420 — Nucleotidyltransferase; domain 5
Homologous superfamily homologous superfamily40 — ATPase, nucleotide binding domain
Domain ID domain_id4jd2A02
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology420 — Nucleotidyltransferase; domain 5
Homologous superfamily homologous superfamily40 — ATPase, nucleotide binding domain
Domain ID domain_id4jd2A03
Class class3 — Alpha Beta
Architecture architecture90 — Alpha-Beta Complex
Topology topology640 — Actin; Chain A, domain 4
Homologous superfamily homologous superfamily10 — ATPase, substrate binding domain, subdomain 4
Domain ID domain_id4jd2B01
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology420 — Nucleotidyltransferase; domain 5
Homologous superfamily homologous superfamily40 — ATPase, nucleotide binding domain
Domain ID domain_id4jd2B02
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology420 — Nucleotidyltransferase; domain 5
Homologous superfamily homologous superfamily40 — ATPase, nucleotide binding domain
Domain ID domain_id4jd2B03
Class class3 — Alpha Beta
Architecture architecture90 — Alpha-Beta Complex
Topology topology640 — Actin; Chain A, domain 4
Homologous superfamily homologous superfamily10 — ATPase, substrate binding domain, subdomain 4
Domain ID domain_id4jd2C00
Class class2 — Mainly Beta
Architecture architecture130 — 7 Propeller
Topology topology10 — Methylamine Dehydrogenase; Chain H
Homologous superfamily homologous superfamily10 — YVTN repeat-like/Quinoprotein amine dehydrogenase
Domain ID domain_id4jd2D01
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology1460 — Yope Regulator; Chain: A,
Homologous superfamily homologous superfamily20 —
Domain ID domain_id4jd2D02
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology1460 — Yope Regulator; Chain: A,
Homologous superfamily homologous superfamily20 —
Domain ID domain_id4jd2E00
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology1760 — Arp2/3 complex 21 kDa subunit ARPC3
Homologous superfamily homologous superfamily10 — Actin-related protein 2/3 complex subunit 3
Domain ID domain_id4jd2F00
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology1460 — Yope Regulator; Chain: A,
Homologous superfamily homologous superfamily20 —
Domain ID domain_id4jd2G00
Class class1 — Mainly Alpha
Architecture architecture25 — Alpha Horseshoe
Topology topology40 — Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat
Homologous superfamily homologous superfamily190 — Actin-related protein 2/3 complex subunit 5
Domain ID domain_id4jd2H00
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology20 — Severin
Homologous superfamily homologous superfamily10 — Severin
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