7t5q

Cryo-EM Structure of a Transition State of Arp2/3 Complex Activation

Method: ELECTRON MICROSCOPY

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Actin-related protein 3

OrganismNot specified

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Insufficient information	Heteromer	Protein 10	Actin-related protein 2 × 1 （A7MB62） Actin-related protein 2/3 complex subunit 1A × 1 （Q1JP79） Actin-related protein 2/3 complex subunit 2 × 1 （Q3MHR7） Actin-related protein 2/3 complex subunit 3 × 1 （Q3T035） Actin-related protein 2/3 complex subunit 4 × 1 （Q148J6） Actin-related protein 2/3 complex subunit 5 × 1 （Q3SYX9） Actin, alpha skeletal muscle × 1 （P68135） F-actin-capping protein subunit alpha-1/Actin nucleation-promoting factor WASL chimera × 1 （P52907,Q91YD9） F-actin-capping protein subunit beta/Actin nucleation-promoting factor WASL chimera × 1 （P47756,Q91YD9） MAGNESIUM ION × 3 ADENOSINE-5'-TRIPHOSPHATE × 2 ADENOSINE-5'-DIPHOSPHATE × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	ARP3_BOVIN
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–418； UniProt 1–418

Actin-related protein 2

OrganismNot specified

UniProt A7MB62

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Insufficient information	Heteromer	Protein 10	Actin-related protein 3 × 1 （P61157） Actin-related protein 2/3 complex subunit 1A × 1 （Q1JP79） Actin-related protein 2/3 complex subunit 2 × 1 （Q3MHR7） Actin-related protein 2/3 complex subunit 3 × 1 （Q3T035） Actin-related protein 2/3 complex subunit 4 × 1 （Q148J6） Actin-related protein 2/3 complex subunit 5 × 1 （Q3SYX9） Actin, alpha skeletal muscle × 1 （P68135） F-actin-capping protein subunit alpha-1/Actin nucleation-promoting factor WASL chimera × 1 （P52907,Q91YD9） F-actin-capping protein subunit beta/Actin nucleation-promoting factor WASL chimera × 1 （P47756,Q91YD9） MAGNESIUM ION × 3 ADENOSINE-5'-TRIPHOSPHATE × 2 ADENOSINE-5'-DIPHOSPHATE × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	ARP2_BOVIN
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain B； PDBConstruct 1–394； UniProt 1–394

Actin-related protein 2/3 complex subunit 1A

OrganismNot specified

UniProt Q1JP79

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Insufficient information	Heteromer	Protein 10	Actin-related protein 3 × 1 （P61157） Actin-related protein 2 × 1 （A7MB62） Actin-related protein 2/3 complex subunit 2 × 1 （Q3MHR7） Actin-related protein 2/3 complex subunit 3 × 1 （Q3T035） Actin-related protein 2/3 complex subunit 4 × 1 （Q148J6） Actin-related protein 2/3 complex subunit 5 × 1 （Q3SYX9） Actin, alpha skeletal muscle × 1 （P68135） F-actin-capping protein subunit alpha-1/Actin nucleation-promoting factor WASL chimera × 1 （P52907,Q91YD9） F-actin-capping protein subunit beta/Actin nucleation-promoting factor WASL chimera × 1 （P47756,Q91YD9） MAGNESIUM ION × 3 ADENOSINE-5'-TRIPHOSPHATE × 2 ADENOSINE-5'-DIPHOSPHATE × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	ARC1A_BOVIN
Isoform	—
PDB entities	3
Chains and sequence ranges	Author chain C； PDBConstruct 1–370； UniProt 1–370

Actin-related protein 2/3 complex subunit 2

OrganismNot specified

UniProt Q3MHR7

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Insufficient information	Heteromer	Protein 10	Actin-related protein 3 × 1 （P61157） Actin-related protein 2 × 1 （A7MB62） Actin-related protein 2/3 complex subunit 1A × 1 （Q1JP79） Actin-related protein 2/3 complex subunit 3 × 1 （Q3T035） Actin-related protein 2/3 complex subunit 4 × 1 （Q148J6） Actin-related protein 2/3 complex subunit 5 × 1 （Q3SYX9） Actin, alpha skeletal muscle × 1 （P68135） F-actin-capping protein subunit alpha-1/Actin nucleation-promoting factor WASL chimera × 1 （P52907,Q91YD9） F-actin-capping protein subunit beta/Actin nucleation-promoting factor WASL chimera × 1 （P47756,Q91YD9） MAGNESIUM ION × 3 ADENOSINE-5'-TRIPHOSPHATE × 2 ADENOSINE-5'-DIPHOSPHATE × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	ARPC2_BOVIN
Isoform	—
PDB entities	4
Chains and sequence ranges	Author chain D； PDBConstruct 1–300； UniProt 1–300

Actin-related protein 2/3 complex subunit 3

OrganismNot specified

UniProt Q3T035

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Insufficient information	Heteromer	Protein 10	Actin-related protein 3 × 1 （P61157） Actin-related protein 2 × 1 （A7MB62） Actin-related protein 2/3 complex subunit 1A × 1 （Q1JP79） Actin-related protein 2/3 complex subunit 2 × 1 （Q3MHR7） Actin-related protein 2/3 complex subunit 4 × 1 （Q148J6） Actin-related protein 2/3 complex subunit 5 × 1 （Q3SYX9） Actin, alpha skeletal muscle × 1 （P68135） F-actin-capping protein subunit alpha-1/Actin nucleation-promoting factor WASL chimera × 1 （P52907,Q91YD9） F-actin-capping protein subunit beta/Actin nucleation-promoting factor WASL chimera × 1 （P47756,Q91YD9） MAGNESIUM ION × 3 ADENOSINE-5'-TRIPHOSPHATE × 2 ADENOSINE-5'-DIPHOSPHATE × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	ARPC3_BOVIN
Isoform	—
PDB entities	5
Chains and sequence ranges	Author chain E； PDBConstruct 1–178； UniProt 1–178

Actin-related protein 2/3 complex subunit 4

OrganismNot specified

UniProt Q148J6

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Insufficient information	Heteromer	Protein 10	Actin-related protein 3 × 1 （P61157） Actin-related protein 2 × 1 （A7MB62） Actin-related protein 2/3 complex subunit 1A × 1 （Q1JP79） Actin-related protein 2/3 complex subunit 2 × 1 （Q3MHR7） Actin-related protein 2/3 complex subunit 3 × 1 （Q3T035） Actin-related protein 2/3 complex subunit 5 × 1 （Q3SYX9） Actin, alpha skeletal muscle × 1 （P68135） F-actin-capping protein subunit alpha-1/Actin nucleation-promoting factor WASL chimera × 1 （P52907,Q91YD9） F-actin-capping protein subunit beta/Actin nucleation-promoting factor WASL chimera × 1 （P47756,Q91YD9） MAGNESIUM ION × 3 ADENOSINE-5'-TRIPHOSPHATE × 2 ADENOSINE-5'-DIPHOSPHATE × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	ARPC4_BOVIN
Isoform	—
PDB entities	6
Chains and sequence ranges	Author chain F； PDBConstruct 1–168； UniProt 1–168

Actin-related protein 2/3 complex subunit 5

OrganismNot specified

UniProt Q3SYX9

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Insufficient information	Heteromer	Protein 10	Actin-related protein 3 × 1 （P61157） Actin-related protein 2 × 1 （A7MB62） Actin-related protein 2/3 complex subunit 1A × 1 （Q1JP79） Actin-related protein 2/3 complex subunit 2 × 1 （Q3MHR7） Actin-related protein 2/3 complex subunit 3 × 1 （Q3T035） Actin-related protein 2/3 complex subunit 4 × 1 （Q148J6） Actin, alpha skeletal muscle × 1 （P68135） F-actin-capping protein subunit alpha-1/Actin nucleation-promoting factor WASL chimera × 1 （P52907,Q91YD9） F-actin-capping protein subunit beta/Actin nucleation-promoting factor WASL chimera × 1 （P47756,Q91YD9） MAGNESIUM ION × 3 ADENOSINE-5'-TRIPHOSPHATE × 2 ADENOSINE-5'-DIPHOSPHATE × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	ARPC5_BOVIN
Isoform	—
PDB entities	7
Chains and sequence ranges	Author chain G； PDBConstruct 1–151； UniProt 1–151

Actin, alpha skeletal muscle

OrganismNot specified

UniProt P68135

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Insufficient information	Heteromer	Protein 10	Actin-related protein 3 × 1 （P61157） Actin-related protein 2 × 1 （A7MB62） Actin-related protein 2/3 complex subunit 1A × 1 （Q1JP79） Actin-related protein 2/3 complex subunit 2 × 1 （Q3MHR7） Actin-related protein 2/3 complex subunit 3 × 1 （Q3T035） Actin-related protein 2/3 complex subunit 4 × 1 （Q148J6） Actin-related protein 2/3 complex subunit 5 × 1 （Q3SYX9） F-actin-capping protein subunit alpha-1/Actin nucleation-promoting factor WASL chimera × 1 （P52907,Q91YD9） F-actin-capping protein subunit beta/Actin nucleation-promoting factor WASL chimera × 1 （P47756,Q91YD9） MAGNESIUM ION × 3 ADENOSINE-5'-TRIPHOSPHATE × 2 ADENOSINE-5'-DIPHOSPHATE × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	ACTS_RABIT
Isoform	—
PDB entities	8
Chains and sequence ranges	Author chain H； PDBConstruct 1–375； UniProt 3–377

F-actin-capping protein subunit alpha-1/Actin nucleation-promoting factor WASL chimera

Mus musculus

UniProt P52907

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Insufficient information	Heteromer	Protein 10	Actin-related protein 3 × 1 （P61157） Actin-related protein 2 × 1 （A7MB62） Actin-related protein 2/3 complex subunit 1A × 1 （Q1JP79） Actin-related protein 2/3 complex subunit 2 × 1 （Q3MHR7） Actin-related protein 2/3 complex subunit 3 × 1 （Q3T035） Actin-related protein 2/3 complex subunit 4 × 1 （Q148J6） Actin-related protein 2/3 complex subunit 5 × 1 （Q3SYX9） Actin, alpha skeletal muscle × 1 （P68135） F-actin-capping protein subunit beta/Actin nucleation-promoting factor WASL chimera × 1 （P47756,Q91YD9） MAGNESIUM ION × 3 ADENOSINE-5'-TRIPHOSPHATE × 2 ADENOSINE-5'-DIPHOSPHATE × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	CAZA1_HUMAN
Isoform	—
PDB entities	9
Chains and sequence ranges	Author chain I； PDBConstruct 1–280； UniProt 1–280

F-actin-capping protein subunit alpha-1/Actin nucleation-promoting factor WASL chimera

Mus musculus

UniProt Q91YD9

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Insufficient information	Heteromer	Protein 10	Actin-related protein 3 × 1 （P61157） Actin-related protein 2 × 1 （A7MB62） Actin-related protein 2/3 complex subunit 1A × 1 （Q1JP79） Actin-related protein 2/3 complex subunit 2 × 1 （Q3MHR7） Actin-related protein 2/3 complex subunit 3 × 1 （Q3T035） Actin-related protein 2/3 complex subunit 4 × 1 （Q148J6） Actin-related protein 2/3 complex subunit 5 × 1 （Q3SYX9） Actin, alpha skeletal muscle × 1 （P68135） MAGNESIUM ION × 3 ADENOSINE-5'-TRIPHOSPHATE × 2 ADENOSINE-5'-DIPHOSPHATE × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	WASL_MOUSE
Isoform	—
PDB entities	9, 10
Chains and sequence ranges	Author chain I； PDBConstruct 281–346； UniProt 436–501 Author chain J； PDBConstruct 272–333； UniProt 440–501

F-actin-capping protein subunit beta/Actin nucleation-promoting factor WASL chimera

Mus musculus

UniProt P47756

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Insufficient information	Heteromer	Protein 10	Actin-related protein 3 × 1 （P61157） Actin-related protein 2 × 1 （A7MB62） Actin-related protein 2/3 complex subunit 1A × 1 （Q1JP79） Actin-related protein 2/3 complex subunit 2 × 1 （Q3MHR7） Actin-related protein 2/3 complex subunit 3 × 1 （Q3T035） Actin-related protein 2/3 complex subunit 4 × 1 （Q148J6） Actin-related protein 2/3 complex subunit 5 × 1 （Q3SYX9） Actin, alpha skeletal muscle × 1 （P68135） F-actin-capping protein subunit alpha-1/Actin nucleation-promoting factor WASL chimera × 1 （P52907,Q91YD9） MAGNESIUM ION × 3 ADENOSINE-5'-TRIPHOSPHATE × 2 ADENOSINE-5'-DIPHOSPHATE × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	CAPZB_HUMAN
Isoform	P47756-1
PDB entities	10
Chains and sequence ranges	Author chain J； PDBConstruct 1–271； UniProt 1–271

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	7t5q
Deposition date deposition_date	2021-12-13
Structure title title	Cryo-EM Structure of a Transition State of Arp2/3 Complex Activation
Keywords keywords	;actin, ATPase, actin related protein, arp, cytoskeleton, Arp2-3 complex, actin nucleation, actin branching, CONTRACTILE PROTEIN, CapZ ;; CONTRACTILE PROTEIN
Experimental Method method	ELECTRON MICROSCOPY

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

7t5q__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

7t5q__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

7t5q__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	52.15 Å
Rg (electron density)	51.82 Å
Total Rg	51.86 Å
Atom count	21671
Residues	2702
Excluded volume	385840 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`7t5q__assembly_1__model_1`	decameric （10）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (13)

Entity 1 Entity 1 polymer

Description description	Actin-related protein 3
Molecular weight formula_weight	47428.0 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A
Atom count atom_count	21671

MAGRLPACVVDCGTGYTKLGYAGNTEPQFIIPSCIAIKESAKVGDQAQRRVMKGVDDLDFFIGDEAIEKPTYATKWPIRHGIVEDWDLMERFMEQVIFKYLRAEPEDHYFLLTEPPLNTPENREYTAEIMFESFNVPGLYIAVQAVLALAASWTSRQVGERTLTGTVIDSGDGVTHVIPVAEGYVIGSCIKHIPIAGRDITYFIQQLLRDREVGIPPEQSLETAKAVKERYSYVCPDLVKEFNKYDTDGSKWIKQYTGINAISKKEFSIDVGYERFLGPEIFFHPEFANPDFTQPISEVVDEVIQNCPIDVRRPLYKNIVLSGGSTMFRDFGRRLQRDLKRTVDARLKLSEELSGGRLKPKPIDVQVITHHMQRYAVWFGGSMLASTPEFYQVCHTKKDYEEIGPSICRHNPVFGVMS

Entity 2 Entity 2 polymer

Description description	Actin-related protein 2
Molecular weight formula_weight	44818.7 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	B

MDSQGRKVVVCDNGTGFVKCGYAGSNFPEHIFPALVGRPIIRSTTKVGNIEIKDLMVGDEASELRSMLEVNYPMENGIVRNWDDMKHLWDYTFGPEKLNIDTRNCKILLTEPPMNPTKNREKIVEVMFETYQFSGVYVAIQAVLTLYAQGLLTGVVVDSGDGVTHICPVYEGFSLPHLTRRLDIAGRDITRYLIKLLLLRGYAFNHSADFETVRMIKEKLCYVGYNIEQEQKLALETTVLVESYTLPDGRIIKVGGERFEAPEALFQPHLINVEGVGVAELLFNTIQAADIDTRSEFYKHIVLSGGSTMYPGLPSRLERELKQLYLERVLKGDVEKLSKFKIRIEDPPRRKHMVFLGGAVLADIMKDKDNFWMTRQEYQEKGVRVLEKLGVTVR

Entity 3 Entity 3 polymer

Description description	Actin-related protein 2/3 complex subunit 1A
Molecular weight formula_weight	41594.2 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	C

MSLHQFLLEPITCHAWNRDRTQIALSPNNHEVHIYKKNGGQWVKAHELKEHNGHITGIDWAPKSDRIVTCGADRNAYVWSQKDGVWKPTLVILRINRAATFVKWSPLENKFAVGSGARLISVCYFESENDWWVSKHIKKPIRSTVLSLDWHPNNVLLAAGSCDFKCRVFSAYIKEVDEKPASTPWGSKMPFGQLMSEFGGSGTGGWVHGVSFSASGSRLAWVSHDSTVSVADASKSVQVSTLKTEFLPLLSVSFVSENSVVAAGHDCCPMLFNYDDRGCLTFVSKLDIPKQSIQRNMSAMERFRNMDKRATTEDRNTALETLHQNSITQVSIYEVDKQDCRKFCTTGIDGAMTIWDFKTLESSIQGLRIM

Entity 4 Entity 4 polymer

Description description	Actin-related protein 2/3 complex subunit 2
Molecular weight formula_weight	34402.0 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	D

MILLEVNNRIIEETLALKFENAAAGNKPEAVEVTFADFDGVLYHISNPNGDKTKVMVSISLKFYKELQAHGADELLKRVYGSYLVNPESGYNVSLLYDLENLPASKDSIVHQAGMLKRNCFASVFEKYFQFQEEGKEGENRAVIHYRDDETMYVESKKDRVTVVFSTVFKDDDDVVIGKVFMQEFKEGRRASHTAPQVLFSHREPPLELKDTDAAVGDNIGYITFVLFPRHTNASARDNTINLIHTFRDYLHYHIKCSKAYIHTRMRAKTSDFLKVLNRARPDAEKKEMKTITGKTFSSR

Entity 5 Entity 5 polymer

Description description	Actin-related protein 2/3 complex subunit 3
Molecular weight formula_weight	20572.7 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	E

MPAYHSSLMDPDTKLIGNMALLPIRSQFKGPAPRETKDTDIVDEAIYYFKANVFFKNYEIKNEADRTLIYITLYISECLKKLQKCNSKSQGEKEMYTLGITNFPIPGEPGFPLNAIYAKPANKQEDEVMRAYLQQLRQETGLRLCEKVFDPQNDKPSKWWTCFVKRQFMNKSLSGPGQ

Entity 6 Entity 6 polymer

Description description	Actin-related protein 2/3 complex subunit 4
Molecular weight formula_weight	19697.0 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	F

MTATLRPYLSAVRATLQAALCLENFSSQVVERHNKPEVEVRSSKELLLQPVTISRNEKEKVLIEGSINSVRVSIAVKQADEIEKILCHKFMRFMMMRAENFFILRRKPVEGYDISFLITNFHTEQMYKHKLVDFVIHFMEEIDKEISEMKLSVNARARIVAEEFLKNF

Entity 7 Entity 7 polymer

Description description	Actin-related protein 2/3 complex subunit 5
Molecular weight formula_weight	16251.3 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	G

MSKNTVSSARFRKVDVGEYDENKFVDEEDGGDGQAGPDEGEVDSCLRQGNMTAALQAALKNPPINTKSQAVKDRAGSIVLKVLISFKANDIEKAVQSLDKNGVDLLMKYIYKGFESPSDNSSAVLLQWHEKALAAGGVGSIVRVLTARKTV

Entity 8 Entity 8 polymer

Description description	Actin, alpha skeletal muscle
Molecular weight formula_weight	41875.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	H

DEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYDEAGPSIVHRKCF

Entity 9 Entity 9 polymer

Description description	F-actin-capping protein subunit alpha-1/Actin nucleation-promoting factor WASL chimera
Molecular weight formula_weight	39600.6 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	I

MADFDDRVSDEEKVRIAAKFITHAPPGEFNEVFNDVRLLLNNDNLLREGAAHAFAQYNMDQFTPVKIEGYEDQVLITEHGDLGNSRFLDPRNKISFKFDHLRKEASDPQPEEADGGLKSWRESCDSALRAYVKDHYSNGFCTVYAKTIDGQQTIIACIESHQFQPKNFWNGRWRSEWKFTITPPTAQVVGVLKIQVHYYEDGNVQLVSHKDVQDSLTVSNEAQTAKEFIKIIENAENEYQTAISENYQTMSDTTFKALRRQLPVTRTKIDWNKILSYKIGQIRQGIQLKSVSDGQESTPPTPAPTSGIVGALMEVMQKRSKAIHSSDEDEDDDDEEDFEDDDEWED

Source Source

Source organism organism	Mus musculus
Common name common_name	house mouse
NCBI taxonomy ID ncbi_taxonomy_id	10090
Host organism host_organism	Escherichia coli

Entity 10 Entity 10 polymer

Description description	F-actin-capping protein subunit beta/Actin nucleation-promoting factor WASL chimera
Molecular weight formula_weight	37470.8 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	J

MSDQQLDCALDLMRRLPPQQIEKNLSDLIDLVPSLCEDLLSSVDQPLKIARDKVVGKDYLLCDYNRDGDSYRSPWSNKYDPPLEDGAMPSARLRKLEVEANNAFDQYRDLYFEGGVSSVYLWDLDHGFAGVILIKKAGDGSKKIKGCWDSIHVVEVQEKSSGRTAHYKLTSTVMLWLQTNKSGSGTMNLGGSLTRQMEKDETVSDCSPHIANIGRLVEDMENKIRSTLNEIYFGKTKDIVNGLRSIDAIPDNQKFKQLQRELSQVLTQRQIGIQLKSVSDGQESTPPTPAPTSGIVGALMEVMQKRSKAIHSSDEDEDDDDEEDFEDDDEWED

Source Source

Source organism organism	Mus musculus
Common name common_name	house mouse
NCBI taxonomy ID ncbi_taxonomy_id	10090
Host organism host_organism	Escherichia coli

Entity 11 Entity 11 non-polymer

Description description	MAGNESIUM ION
Molecular weight formula_weight	24.3 kDa
Source method src_method	syn
Copy count entity_count	3

Entity 12 Entity 12 non-polymer

Description description	ADENOSINE-5'-TRIPHOSPHATE
Molecular weight formula_weight	507.2 kDa
Source method src_method	syn
Copy count entity_count	2

Entity 13 Entity 13 non-polymer

Description description	ADENOSINE-5'-DIPHOSPHATE
Molecular weight formula_weight	427.2 kDa
Source method src_method	syn
Copy count entity_count	1

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6. Fold Classification (SCOP + CATH) 3 domains

CATH v4.4 (3 domains)

Domain ID domain_id	7t5qC01
Class class	2 — Mainly Beta
Architecture architecture	130 — 7 Propeller
Topology topology	10 — Methylamine Dehydrogenase; Chain H
Homologous superfamily homologous superfamily	10 — YVTN repeat-like/Quinoprotein amine dehydrogenase

Domain ID domain_id	7t5qD01
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	1460 — Yope Regulator; Chain: A,
Homologous superfamily homologous superfamily	20 —

Domain ID domain_id	7t5qD02
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	1460 — Yope Regulator; Chain: A,
Homologous superfamily homologous superfamily	20 —

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Unit-cell length a length_a	1.000 Å
Unit-cell length b length_b	1.000 Å
Unit-cell length c length_c	1.000 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	—

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	—

Resolution resolution	3.40 Å
Resolution测定Method resolution_method	—
颗粒数 num_particles	269760

Cryo-EM Structure of a Transition State of Arp2/3 Complex Activation

1. Protein Identity and Related Structures Protein Identity & Related Structures

Actin-related protein 3

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Actin-related protein 2

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Actin-related protein 2/3 complex subunit 1A

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Actin-related protein 2/3 complex subunit 2

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Actin-related protein 2/3 complex subunit 3

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Actin-related protein 2/3 complex subunit 4

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Actin-related protein 2/3 complex subunit 5

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Actin, alpha skeletal muscle

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

F-actin-capping protein subunit alpha-1/Actin nucleation-promoting factor WASL chimera

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

F-actin-capping protein subunit alpha-1/Actin nucleation-promoting factor WASL chimera

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer