1o18

MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE

Method: ELECTRON MICROSCOPY D_max: 302.8 Å Quality: EXCELLENT

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	1o18
沉积日期 deposition_date	2002-11-04
结构标题 title	MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE
关键词 keywords	ACTIN-MYOSIN COMPLEX IN SITU IN MUSCLE, CONTRACTILE PROTEIN; CONTRACTILE PROTEIN
实验方法 method	ELECTRON MICROSCOPY

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	— Å
回转半径 Rg (电子) rg_electron	116.50 Å
零角强度 I(0) i0	23158600000.00
分子量 molecular_weight	1319000.0 kDa
排除体积 excluded_volume	1657800 Å³
包络体积 envelope_volume	3229900 Å³
水化壳体积 shell_volume	241810 Å³
包络直径 envelope_diameter	422.7 Å
壳层 Rg shell_rg	90.16 Å
包络 Rg envelope_rg	113.30 Å
形状 Rg shape_rg	116.50 Å
总 Rg total_rg	116.40 Å
总原子数 total_atoms	92716
残基数 n_residues	11438
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	302.8 Å
Rg (实空间) rg_real	110.10 Å
Rg 误差 (实空间) rg_real_error	1.16 Å
I(0) (实空间) i0_real	2.2210e+10
I(0) 误差 (实空间) i0_real_error	4.9180e+08
Rg (倒空间) rg_reciprocal	109.30 Å
I(0) (倒空间) i0_reciprocal	22610000000.0000
解质量估计 total_estimate	0.9153
解质量评级 solution_quality	EXCELLENT a EXCELLENT solution
P(r) 峰数 n_peaks	1
主峰位置 r_peak_primary	116.4 Å
偏度 Skewness skewness	0.192
峰度 Kurtosis kurtosis	-0.706
角度范围 angular_range	— – 0.0650 Å⁻¹
当前正则化参数 α current_alpha	0.5418
最高正则化参数 α highest_alpha	507700000.0000
实空间数据点数 n_real_points	14
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.003; Oscil: 0.995; Stabil: 0.983; Sysdev: 1.000; Positv: 1.000; Valcen: 0.968; Smooth: 0.000

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (4)

Entity 1 polymer

描述 description	SKELETAL MUSCLE MYOSIN II
分子量 formula_weight	96625.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	6

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A,D,G,J,M,P
原子数 atom_count	92716

DAEMAAFGEAAPYLRKSEKERIEAQNKPFDAKSSVFVVHPKQSFVKGTIQSKEGGKVTVKTEGGETLTVKEDQVFSMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNPKVVLAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAASGEKKKEEQSGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVTFQLPAERSYHIFYQIMSNKKPELIDMLLITTNPYDYHYVSEGEITVPSIDDQEELMATDSAIDILGFSADEKTAIYKLTGAVMHYGNLKFKQKQREEQAEPDGTEVADKAAYLMGLNSAELLKALCYPRVGVGNEAVTKGETVSEVHNSVGALAKAVYEKMFLWMVIRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYDEHLGKSNNFQKPKPAKGKAEAHFSLVHYAGTVDYNISGWLEKNKDPLNETVIGLYQKSSVKTLALLFATYGGEAEGGGGKKGGKKKGSSFQTVSALFRENLNKLMANLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRVLYADFKQRYRVLNASAIPEGQFMDSKKASEKLLGGGDVDHTQYAFGHTKVFFKAGLLGLLEEMRDDKLAEIITATQARCRGFLMRVEYRAMVERRESIFCIQYNVRSFMNVKHWPWMKLFFKIKPLLK

Entity 2 polymer

描述 description	SKELETAL MUSCLE MYOSIN II REGULATORY LIGHT CHAIN
分子量 formula_weight	16063.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	5

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	E,H,K,N,Q

FDETEIEDFKEAFTVIDQNADGIIDKDDLRETFAAMGRLNVKNEELDAMIKEASGPINFTVFLTMFGEKLKGADPEDVIMGAFKVLDPDGKGSIKKSFLEELLTTGGGRFTPEEIKNMWAAFPPDVAGNVDYKNICYVITHGEDA

Entity 3 polymer

描述 description	SKELETAL MUSCLE MYOSIN II ESSENTIAL LIGHT CHAIN
分子量 formula_weight	16063.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	5

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	F,I,L,O,R

SKAAADDFKEAFLLFDRTGDAKITASQVGDIARALGQNPTNAEINKILGNPSKEEMNAAAITFEEFLPMLQAAANNKDQGTFEDFVEGLRVFDKEGNGTVMGAELRHVLATLGEKMTEEEVEELMKGQEDSNGCINYEAFVKHIMSV

Entity 4 polymer

描述 description	SKELETAL MUSCLE ACTIN
分子量 formula_weight	41862.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	14

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	1,2,3,4,5,6,7,8,9,V,W,X,Y,Z

DEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYDEAGPSIVHRKCF

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6. 折叠分类 (SCOP + CATH) 30 domains

SCOP 2.08 (30 domains)

结构域编号 domain_id	d1o181_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o182_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o183_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o184_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o185_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o186_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o187_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o188_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o189_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o18a_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o18d_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o18e_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o18f_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o18g_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o18h_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o18i_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o18j_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o18k_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o18l_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o18m_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o18n_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o18o_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o18p_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o18q_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o18r_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o18v_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o18w_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o18x_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o18y_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o18z_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

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7. 引用文献 (4)

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晶胞边长 a length_a	1.000 Å
晶胞边长 b length_b	1.000 Å
晶胞边长 c length_c	1.000 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	1

空间群 space_group	P 1
空间群编号 int_tables_number	1
晶系 crystal_system	—

分辨率 resolution	70.000 Å
最低分辨率 resolution_low	— Å
R 因子 r_factor	—
R_work r_work	—
R_free r_free	—

分辨率 resolution	70.00 Å
分辨率测定方法 resolution_method	DUAL AXIS TILT SERIES ELECTRON TOMOGRAPHY
颗粒数 num_particles	—

fit	/app/data/saxs_computation/o1/1o18/1o18.fit
pddf	/app/data/pr_computation/o1/1o18/1o18.pr.out
profile	/app/data/saxs_computation/o1/1o18/1o18.dat

MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

精修参数 Refinement

冷冻电镜 EM 3D

5. 实体与聚合体信息 (4)

Entity 1 polymer

聚合体 Polymer

Entity 2 polymer

聚合体 Polymer

Entity 3 polymer

聚合体 Polymer

Entity 4 polymer

聚合体 Polymer

6. 折叠分类 (SCOP + CATH) 30 domains

SCOP 2.08 (30 domains)

7. 引用文献 (4)

8. 文件与曲线 (10)

Files (7)

Curves (3)