Actin, alpha skeletal muscle
OrganismNot specified
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Protein heterocomplex Heteromer Protein × 6 PDB declaration: hexameric（6） Consistent with protein copy count | Chain A； UniProt 3–377 Chain B； UniProt 3–377 Chain C； UniProt 3–377 Chain D； UniProt 3–377 Chain E； UniProt 3–377 | Not recorded | MAL × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 5 CA CALCIUM ION × 5 | X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION；pH 5.3；293 K；50mM citrate, 0.1M ammonium sulfate, 0.1mM CaCl2, 0.1mM ATP, 10% PEG8000, pH 5.3, VAPOR DIFFUSION, temperature 293K | Resolution 3.45 Å R-free 0.273 |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 3TPQ | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1EQY COMPLEX BETWEEN RABBIT MUSCLE ALPHA-ACTIN: HUMAN GELSOLIN DOMAIN 1 Deposited 2000-04-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 6.6；298 K；PEG 6000, sodium chloride, adenosine triphosphate, calcium, magnesium, sodium azide, pH 6.6, Vapor Diffusion, temperature 298.0K
|
Resolution 2.30 Å R-free 0.280 |
| 1ESV COMPLEX BETWEEN LATRUNCULIN A:RABBIT MUSCLE ALPHA ACTIN:HUMAN GELSOLIN DOMAIN 1 Deposited 2000-04-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 LAR LATRUNCULIN A × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 6.6；298 K；PEG 6000, sodium Chloride, adenosine triphosphate, calcium, magnesium, sodium azide, pH 6.6, VAPOR DIFFUSION, temperature 298.0K
|
Resolution 2.00 Å R-free 0.286 |
| 1IJJ THE X-RAY CRYSTAL STRUCTURE OF THE COMPLEX BETWEEN RABBIT SKELETAL MUSCLE ACTIN AND LATRUNCULIN A AT 2.85 A RESOLUTION Deposited 2001-04-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–377（377 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 LAR LATRUNCULIN A × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；298 K；ammonium sulfate, magnesium chloride, pH 6.8,
VAPOR DIFFUSION, HANGING DROP at 298 K
|
Resolution 2.85 Å R-free 0.309 |
| 1IJJ THE X-RAY CRYSTAL STRUCTURE OF THE COMPLEX BETWEEN RABBIT SKELETAL MUSCLE ACTIN AND LATRUNCULIN A AT 2.85 A RESOLUTION Deposited 2001-04-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–377（377 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 LAR LATRUNCULIN A × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；298 K；ammonium sulfate, magnesium chloride, pH 6.8,
VAPOR DIFFUSION, HANGING DROP at 298 K
|
Resolution 2.85 Å R-free 0.309 |
| 1J6Z UNCOMPLEXED ACTIN Deposited 2001-05-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 RHO TETRAMETHYLRHODAMINE-5-MALEIMIDE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；PEG 2000 MONOMETHYLETHER 22%, CALCIUM ACETATE 200mM, TRIS 10mM (pH 7), pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.54 Å R-free 0.223 |
| 1KXP CRYSTAL STRUCTURE OF HUMAN VITAMIN D-BINDING PROTEIN IN COMPLEX WITH SKELETAL ACTIN Deposited 2002-02-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.6；277 K；Peg 8K 12%, Magnesium Acetate 200mM, Sodium Cacodylate 100 mM, Glycerol 20%, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.10 Å R-free 0.236 |
| 1LCU Polylysine Induces an Antiparallel Actin Dimer that Nucleates Filament Assembly: Crystal Structure at 3.5 A Resolution Deposited 2002-04-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
7–377（371 aa）
Chain B
7–377（371 aa）
|
Not recorded | CA CALCIUM ION × 4 CL CHLORIDE ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 LAR LATRUNCULIN A × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；Ammonium sulfate, MgCl2, Imidazole, ATP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.50 Å R-free 0.266 |
| 1LOT CRYSTAL STRUCTURE OF THE COMPLEX OF ACTIN WITH VITAMIN D-BINDING PROTEIN Deposited 2002-05-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | GOL GLYCEROL × 6 CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；290 K；PEG8000, cacodylate, calcium acetate, glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K
|
Resolution 2.50 Å R-free 0.279 |
| 1M8Q Molecular Models of Averaged Rigor Crossbridges from Tomograms of Insect Flight Muscle Deposited 2002-07-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 26 PDB declaration: 26-meric |
Chain 0
1–375（375 aa）
Chain 1
1–375（375 aa）
Chain 2
1–375（375 aa）
Chain 3
1–375（375 aa）
Chain 4
1–375（375 aa）
Chain 5
1–375（375 aa）
Chain 7
1–375（375 aa）
Chain 8
1–375（375 aa）
Chain 9
1–375（375 aa）
Chain V
1–375（375 aa）
Chain W
1–375（375 aa）
Chain X
1–375（375 aa）
Chain Y
1–375（375 aa）
Chain Z
1–375（375 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM vitrification conditions
No vitrification. Samples were viewed at room temperature.
|
Resolution 70.00 Å |
| 1MA9 Crystal structure of the complex of human vitamin D binding protein and rabbit muscle actin Deposited 2002-08-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
3–372（370 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.3；277 K；PEG 8000, magnesium acetate, sodium cacodylate, glycerol, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.40 Å R-free 0.250 |
| 1MVW MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE Deposited 2002-09-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric |
Chain 1
3–377（375 aa）
Chain 2
3–377（375 aa）
Chain 3
3–377（375 aa）
Chain 4
3–377（375 aa）
Chain 5
3–377（375 aa）
Chain 6
3–377（375 aa）
Chain 7
3–377（375 aa）
Chain 8
3–377（375 aa）
Chain 9
3–377（375 aa）
Chain V
3–377（375 aa）
Chain W
3–377（375 aa）
Chain X
3–377（375 aa）
Chain Y
3–377（375 aa）
Chain Z
3–377（375 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM vitrification conditions
NO VITRIFICATION. SAMPLES WERE VIEWED AT ROOM TEMPERATURE.
|
Resolution 70.00 Å |
| 1NWK CRYSTAL STRUCTURE OF MONOMERIC ACTIN IN THE ATP STATE Deposited 2003-02-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 4 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 RHO TETRAMETHYLRHODAMINE-5-MALEIMIDE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；PEG 3350, CaCl2, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 393K
|
Resolution 1.85 Å R-free 0.226 |
| 1O18 MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE Deposited 2002-11-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric |
Chain 1
3–377（375 aa）
Chain 2
3–377（375 aa）
Chain 3
3–377（375 aa）
Chain 4
3–377（375 aa）
Chain 5
3–377（375 aa）
Chain 6
3–377（375 aa）
Chain 7
3–377（375 aa）
Chain 8
3–377（375 aa）
Chain 9
3–377（375 aa）
Chain V
3–377（375 aa）
Chain W
3–377（375 aa）
Chain X
3–377（375 aa）
Chain Y
3–377（375 aa）
Chain Z
3–377（375 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM vitrification conditions
NO VITRIFICATION. SAMPLES WERE VIEWED AT ROOM TEMPERATURE.
|
Resolution 70.00 Å |
| 1O19 MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE Deposited 2002-11-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric |
Chain 1
3–377（375 aa）
Chain 2
3–377（375 aa）
Chain 3
3–377（375 aa）
Chain 4
3–377（375 aa）
Chain 5
3–377（375 aa）
Chain 6
3–377（375 aa）
Chain 7
3–377（375 aa）
Chain 8
3–377（375 aa）
Chain 9
3–377（375 aa）
Chain V
3–377（375 aa）
Chain W
3–377（375 aa）
Chain X
3–377（375 aa）
Chain Y
3–377（375 aa）
Chain Z
3–377（375 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM vitrification conditions
NO VITRIFICATION. SAMPLES WERE VIEWED AT ROOM TEMPERATURE.
|
Resolution 70.00 Å |
| 1O1A MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE Deposited 2002-11-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric |
Chain 1
3–377（375 aa）
Chain 2
3–377（375 aa）
Chain 3
3–377（375 aa）
Chain 4
3–377（375 aa）
Chain 5
3–377（375 aa）
Chain 6
3–377（375 aa）
Chain 7
3–377（375 aa）
Chain 8
3–377（375 aa）
Chain 9
3–377（375 aa）
Chain V
3–377（375 aa）
Chain W
3–377（375 aa）
Chain X
3–377（375 aa）
Chain Y
3–377（375 aa）
Chain Z
3–377（375 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM vitrification conditions
NO VITRIFICATION. SAMPLES WERE VIEWED AT ROOM TEMPERATURE.
|
Resolution 70.00 Å |
| 1O1B MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE Deposited 2002-11-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 26 PDB declaration: 26-meric |
Chain 0
3–377（375 aa）
Chain 1
3–377（375 aa）
Chain 2
3–377（375 aa）
Chain 3
3–377（375 aa）
Chain 4
3–377（375 aa）
Chain 5
3–377（375 aa）
Chain 7
3–377（375 aa）
Chain 8
3–377（375 aa）
Chain 9
3–377（375 aa）
Chain V
3–377（375 aa）
Chain W
3–377（375 aa）
Chain X
3–377（375 aa）
Chain Y
3–377（375 aa）
Chain Z
3–377（375 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM vitrification conditions
NO VITRIFICATION. SAMPLES WERE VIEWED AT ROOM TEMPERATURE.
|
Resolution 70.00 Å |
| 1O1C MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE Deposited 2002-11-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 29 PDB declaration: 29-meric |
Chain 0
3–377（375 aa）
Chain 1
3–377（375 aa）
Chain 2
3–377（375 aa）
Chain 3
3–377（375 aa）
Chain 4
3–377（375 aa）
Chain 5
3–377（375 aa）
Chain 7
3–377（375 aa）
Chain 8
3–377（375 aa）
Chain 9
3–377（375 aa）
Chain V
3–377（375 aa）
Chain W
3–377（375 aa）
Chain X
3–377（375 aa）
Chain Y
3–377（375 aa）
Chain Z
3–377（375 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM vitrification conditions
NO VITRIFICATION. SAMPLES WERE VIEWED AT ROOM TEMPERATURE.
|
Resolution 70.00 Å |
| 1O1D MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE Deposited 2002-11-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric |
Chain 0
3–377（375 aa）
Chain 1
3–377（375 aa）
Chain 2
3–377（375 aa）
Chain 3
3–377（375 aa）
Chain 4
3–377（375 aa）
Chain 5
3–377（375 aa）
Chain 7
3–377（375 aa）
Chain 8
3–377（375 aa）
Chain 9
3–377（375 aa）
Chain V
3–377（375 aa）
Chain W
3–377（375 aa）
Chain X
3–377（375 aa）
Chain Y
3–377（375 aa）
Chain Z
3–377（375 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM vitrification conditions
NO VITRIFICATION. SAMPLES WERE VIEWED AT ROOM TEMPERATURE.
|
Resolution 70.00 Å |
| 1O1E MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE Deposited 2002-11-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric |
Chain 1
3–377（375 aa）
Chain 2
3–377（375 aa）
Chain 3
3–377（375 aa）
Chain 4
3–377（375 aa）
Chain 5
3–377（375 aa）
Chain 6
3–377（375 aa）
Chain 7
3–377（375 aa）
Chain 8
3–377（375 aa）
Chain 9
3–377（375 aa）
Chain V
3–377（375 aa）
Chain W
3–377（375 aa）
Chain X
3–377（375 aa）
Chain Y
3–377（375 aa）
Chain Z
3–377（375 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM vitrification conditions
NO VITRIFICATION. SAMPLES WERE VIEWED AT ROOM TEMPERATURE.
|
Resolution 70.00 Å |
| 1O1F MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE Deposited 2002-11-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 26 PDB declaration: 26-meric |
Chain 0
3–377（375 aa）
Chain 1
3–377（375 aa）
Chain 2
3–377（375 aa）
Chain 3
3–377（375 aa）
Chain 4
3–377（375 aa）
Chain 5
3–377（375 aa）
Chain 6
3–377（375 aa）
Chain 7
3–377（375 aa）
Chain 8
3–377（375 aa）
Chain V
3–377（375 aa）
Chain W
3–377（375 aa）
Chain X
3–377（375 aa）
Chain Y
3–377（375 aa）
Chain Z
3–377（375 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM vitrification conditions
NO VITRIFICATION. SAMPLES WERE VIEWED AT ROOM TEMPERATURE.
|
Resolution 70.00 Å |
| 1O1G MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE Deposited 2002-11-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric |
Chain 1
3–377（375 aa）
Chain 2
3–377（375 aa）
Chain 3
3–377（375 aa）
Chain 4
3–377（375 aa）
Chain 5
3–377（375 aa）
Chain 6
3–377（375 aa）
Chain 7
3–377（375 aa）
Chain 8
3–377（375 aa）
Chain 9
3–377（375 aa）
Chain V
3–377（375 aa）
Chain W
3–377（375 aa）
Chain X
3–377（375 aa）
Chain Y
3–377（375 aa）
Chain Z
3–377（375 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM vitrification conditions
NO VITRIFICATION. SAMPLES WERE VIEWED AT ROOM TEMPERATURE.
|
Resolution 70.00 Å |
| 1P8Z Complex Between Rabbit Muscle alpha-Actin: Human Gelsolin Residues Val26-Glu156 Deposited 2003-05-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–377（377 aa）
|
Not recorded | CD CADMIUM ION × 3 CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；293 K；0.1M Sodium Acetate, 10 mM Cadmium Chloride, 12.5% (v/v) PEG 400, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.60 Å R-free 0.263 |
| 1P8Z Complex Between Rabbit Muscle alpha-Actin: Human Gelsolin Residues Val26-Glu156 Deposited 2003-05-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–377（377 aa）
|
Not recorded | CD CADMIUM ION × 6 CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；293 K；0.1M Sodium Acetate, 10 mM Cadmium Chloride, 12.5% (v/v) PEG 400, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.60 Å R-free 0.263 |
| 1QZ5 Structure of rabbit actin in complex with kabiramide C Deposited 2003-09-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 KAB KABIRAMIDE C × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
Batch；pH 5.5；293 K；Crystals of the actin-kabiramide C complex were grown by small-scale batch by mixing equal volumes of the complex and 100 mM MES, 18% (w/v) polyethylene glycol 1500, 12% (w/v) 1,6-hexanediol, 100 mM CaCl2, 1 mM sodium azide, 1 mM TCEP, pH 5.5, Batch, temperature 293K
|
Resolution 1.45 Å R-free 0.187 |
| 1QZ6 Structure of rabbit actin in complex with jaspisamide A Deposited 2003-09-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 JAS JASPISAMIDE A × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
Batch；pH 7；293 K；Crystals were grown by small-scale batch by mixing equal volumes of the complex (10 mg/ml protein) and 100 mM bis-tris propane, 15% (w/v) dimethyl polyethylene glycol 5000, 25 mM CaCl2, 1 mM sodium azide, 1 mM TCEP, pH 7.0, Batch, temperature 293K
|
Resolution 1.60 Å R-free 0.197 |
| 1RDW Actin Crystal Dynamics: Structural Implications for F-actin Nucleation, Polymerization and Branching Mediated by the Anti-parallel Dimer Deposited 2003-11-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain X
3–377（375 aa）
Fragment：ACtin
|
Not recorded | MG MAGNESIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 LAR LATRUNCULIN A × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.7；MgCl2, NH4SO4, pH 6.7, VAPOR DIFFUSION, HANGING DROP
|
Resolution 2.30 Å R-free 0.224 |
| 1RFQ Actin Crystal Dynamics: Structural Implications for F-actin Nucleation, Polymerization and Branching Mediated by the Anti-parallel Dimer Deposited 2003-11-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
Fragment：actin
Chain B
3–377（375 aa）
Fragment：actin
|
Not recorded | MG MAGNESIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 LAR LATRUNCULIN A × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.7；MgCl2, NH4SO4, pH 6.7, VAPOR DIFFUSION, HANGING DROP
|
Resolution 3.00 Å R-free 0.261 |
| 1RGI Crystal structure of gelsolin domains G1-G3 bound to actin Deposited 2003-11-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–377（377 aa）
|
Not recorded | CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；277 K；2% PEG 8000, 100 mM Sodium acetate, 1 mM CaCl2, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 3.00 Å R-free 0.258 |
| 1S22 Absolute Stereochemistry of Ulapualide A Deposited 2004-01-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–375（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 ULA ULAPUALIDE A × 1 EDO 1,2-ETHANEDIOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
Batch；pH 6；293 K；100 mM MES, 15% methyl ether poly(ethylene glycol) 5000, 75 mM CaCl2, 1 mM sodium azide, 1 mM TCEP, pH 6.0, Batch, temperature 293K
|
Resolution 1.60 Å R-free 0.179 |
| 1SQK CRYSTAL STRUCTURE OF CIBOULOT IN COMPLEX WITH SKELETAL ACTIN Deposited 2004-03-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–377（377 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 LAR LATRUNCULIN A × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；PEG 8000 20%, MAGNESIUM ACETATE 200MM, SODIUM CACODYLATE 100 MM, pH 6.50, VAPOR DIFFUSION, HANGING DROP
|
Resolution 2.50 Å R-free 0.278 |
| 1WUA The structure of Aplyronine A-actin complex Deposited 2004-12-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 AP8 (8R,9R,10R,11R,14S,18S,20S,24S)-24-{(1R,2S,3R,6R,7R,8R,9S,10E)-8-(ACETYLOXY)-6-[(N,N-DIMETHYLALANYL)OXY]-11-[FORMYL(MET HYL)AMINO]-2-HYDROXY-1,3,7,9-TETRAMETHYLUNDEC-10-ENYL}-10-HYDROXY-14,20-DIMETHOXY-9,11,15,18-TETRAMETHYL-2-OXOOXACYCLOTE TRACOSA-3,5,15,21-TETRAEN-8-YL N,N,O-TRIMETHYLSERINATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；PEG8000 20%(w/v) 0.1M sodium cacodylate , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.45 Å R-free 0.176 |
| 1Y64 Bni1p Formin Homology 2 Domain complexed with ATP-actin Deposited 2004-12-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.6；298 K；potassium bromide, hepes, DTT, Tris, ATP, calcium chloride, ethylene glycol, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.05 Å R-free 0.313 |
| 1YXQ Crystal structure of actin in complex with swinholide A Deposited 2005-02-22 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 EDO 1,2-ETHANEDIOL × 2 SWI SWINHOLIDE A × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
small-scale batch；pH 8.5；277 K；dimethyl PEG 5000, HEPPS, MgCl2, TCEP, NaN3, pH 8.5, small-scale batch, temperature 277K
|
Resolution 2.01 Å R-free 0.219 |
| 2A3Z Ternary complex of the WH2 domain of WASP with Actin-DNAse I Deposited 2005-06-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GOL GLYCEROL × 3 MG MAGNESIUM ION × 1 FMT FORMIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.2；293 K；sodium formate, PEG2000 MME, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.08 Å R-free 0.210 |
| 2A40 Ternary complex of the WH2 domain of WAVE with Actin-DNAse I Deposited 2005-06-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GOL GLYCEROL × 2 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.6；293 K；sodium formate, PEG3350, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.80 Å R-free 0.215 |
| 2A40 Ternary complex of the WH2 domain of WAVE with Actin-DNAse I Deposited 2005-06-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Other combination Heteromer；Protein × 3 PDB declaration: trimeric |
Chain D
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.6；293 K；sodium formate, PEG3350, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.80 Å R-free 0.215 |
| 2A40 Ternary complex of the WH2 domain of WAVE with Actin-DNAse I Deposited 2005-06-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain A
3–377（375 aa）
Chain D
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 GOL GLYCEROL × 2 MG MAGNESIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.6；293 K；sodium formate, PEG3350, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.80 Å R-free 0.215 |
| 2A41 Ternary complex of the WH2 Domain of WIP with Actin-DNAse I Deposited 2005-06-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 FMT FORMIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；sodium formate, PEG3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.60 Å R-free 0.219 |
| 2A42 Actin-DNAse I Complex Deposited 2005-06-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GOL GLYCEROL × 3 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；sodium formate, PEG3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.85 Å R-free 0.199 |
| 2A5X Crystal Structure of a Cross-linked Actin Dimer Deposited 2005-07-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
|
Not recorded | CA CALCIUM ION × 4 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 LAR LATRUNCULIN A × 2 NSB N,N,N-TRIMETHYL-3-SULFOPROPAN-1-AMINIUM × 2 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.7；293 K；35% MPD, 100 mM sodium acetate pH 4.7, 20 mM calcium chloride, non-detergent sulfo-betaine, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.49 Å R-free 0.250 |
| 2ASM Structure of Rabbit Actin In Complex With Reidispongiolide A Deposited 2005-08-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–377（375 aa）
|
Not recorded | CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 RGA REIDISPONGIOLIDE A × 1 EDO 1,2-ETHANEDIOL × 5 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；100mM Na/MES/Acetate, pH 5.5, 7% methyl ether poly(ethylene glycol) 5000, 100mM CaCl2, 1mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.60 Å R-free 0.189 |
| 2ASO Structure of Rabbit Actin In Complex With Sphinxolide B Deposited 2005-08-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 SPX SPHINXOLIDE B × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；100mM Na/MES/Acetate, pH 5.5, 12% methyl ether poly(ethylene glycol) 5000, 100mM CaCl2, 1mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.70 Å R-free 0.213 |
| 2ASP Structure of Rabbit Actin In Complex With Reidispongiolide C Deposited 2005-08-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–377（375 aa）
|
Not recorded | CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 RGC REIDISPONGIOLIDE C × 1 EDO 1,2-ETHANEDIOL × 5 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；50mM MES, pH 6.5, 12% methyl ether poly(ethylene glycol) 5000, 40mM MgCl2, 10% ethylene glycol, 1mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.64 Å R-free 0.193 |
| 2D1K Ternary complex of the WH2 domain of mim with actin-dnase I Deposited 2005-08-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；sodium formate, PEG3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.50 Å R-free 0.284 |
| 2FF3 Crystal structure of Gelsolin domain 1:N-wasp V2 motif hybrid in complex with actin Deposited 2005-12-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain B
3–377（375 aa）
|
Not recorded | CA CALCIUM ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
MICROBATCH；pH 6.5；293.15 K；5% PEG 8000, 0.1M sodium acetate, 10mM calcium chloride, pH 6.5, microbatch, temperature 293.15K
|
Resolution 2.00 Å R-free 0.250 |
| 2FF6 Crystal structure of Gelsolin domain 1:ciboulot domain 2 hybrid in complex with actin Deposited 2005-12-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
3–377（375 aa）
|
Not recorded | CA CALCIUM ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293.15 K；7% PEG 3000, 0.1M Hepes, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K
|
Resolution 2.05 Å R-free 0.237 |
| 2FXU X-ray Structure of Bistramide A- Actin Complex at 1.35 A resolution. Deposited 2006-02-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 BID BISTRAMIDE A × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 6；298 K；The bistramide A- actin complex was mixed with the crystallization buffer in 1:1 ratio. The crystallization buffer is 100 mM MES (ph 6.0), 24% (w/v) PEG1500, 70 mM CaCl2, 1mM NaN3, 1mM TCEP., VAPOR DIFFUSION, temperature 298K
|
Resolution 1.35 Å R-free 0.201 |
| 2GWJ SpvB ADP-ribosylated actin: hexagonal crystal form Deposited 2006-05-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
7–377（371 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.90 Å R-free 0.184 |
| 2GWK SpvB ADP-ribosylated actin: orthorhombic crystal form Deposited 2006-05-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
7–377（371 aa）
Chain B
7–377（371 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.00 Å R-free 0.211 |
| 2HMP Uncomplexed actin cleaved with protease ECP32 Deposited 2006-07-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | SR STRONTIUM ION × 7 SPD SPERMIDINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 EDO 1,2-ETHANEDIOL × 9 211 2,2',2''-NITRILOTRIETHANOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.75；277 K；1:1 mixture of 5-7.5 mg/ml protein solution containing 0.5 mM ATP and
precipitant solution (40 mM SrCl2, 10% ethylene glycol, 13-15%
dimethyl polyethylene glycol 5000, 50 mM triethanolamine, 10 mM
spermidine, pH 7.75) equilibrated against 0.5 ml precipitant., VAPOR
DIFFUSION, HANGING DROP, temperature 277K, VAPOR DIFFUSION, HANGING DROP
|
Resolution 1.90 Å R-free 0.215 |
| 2PAV Ternary complex of Profilin-Actin with the Last Poly-Pro of Human VASP Deposited 2007-03-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；277.15 K；200 mM sodium formate, 20% PEG 3350, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K
|
Resolution 1.80 Å R-free 0.208 |
| 2PBD Ternary complex of profilin-actin with the poly-PRO-GAB domain of VASP* Deposited 2007-03-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
1–377（377 aa）
Fragment：residues 1-377
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；277.15 K；150mM DL-malic acid pH 7.0, 18% PEG 3350, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K
|
Resolution 1.50 Å R-free 0.190 |
| 2Q0R Structure of Pectenotoxin-2 Bound to Actin Deposited 2007-05-22 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–377（375 aa）
|
Not recorded | CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 PXT PECTENOTOXIN-2 × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；100 mM Na/MES/acetate, pH 5.5, 15% methyl ether poly(ethylene glycol) 5000, 10% hexanediol, 30 mM CaCl2, and 1 mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.70 Å R-free 0.204 |
| 2Q0U Structure of Pectenotoxin-2 and Latrunculin B Bound to Actin Deposited 2007-05-22 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 LAB LATRUNCULIN B × 1 PXT PECTENOTOXIN-2 × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；100 mM Na/MES/acetate, pH 5.5, 15% methyl ether poly(ethylene glycol) 5000, 10% hexanediol, 100 mM CaCl2, and 1 mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.45 Å R-free 0.181 |
| 2Q1N Actin Dimer Cross-linked Between Residues 41 and 374 Deposited 2007-05-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
|
Not recorded | CA CALCIUM ION × 4 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 LAR LATRUNCULIN A × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；298 K；30% MPD, 0.1M sodium acetate, 0.02 M calcium chloride, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.70 Å R-free 0.277 |
| 2Q1N Actin Dimer Cross-linked Between Residues 41 and 374 Deposited 2007-05-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain B
3–377（375 aa）
|
Not recorded | CA CALCIUM ION × 4 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 LAR LATRUNCULIN A × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；298 K；30% MPD, 0.1M sodium acetate, 0.02 M calcium chloride, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.70 Å R-free 0.277 |
| 2Q31 Actin Dimer Cross-linked Between Residues 41 and 374 and proteolytically cleaved by subtilisin between residues 47 and 48. Deposited 2007-05-29 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
|
Not recorded | CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 LAR LATRUNCULIN A × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；298 K；30% MPD, 0.1 M sodium acetate, 0.01M calcium chloride, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.70 Å R-free 0.288 |
| 2Q31 Actin Dimer Cross-linked Between Residues 41 and 374 and proteolytically cleaved by subtilisin between residues 47 and 48. Deposited 2007-05-29 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain B
3–377（375 aa）
|
Not recorded | CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 LAR LATRUNCULIN A × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；298 K；30% MPD, 0.1 M sodium acetate, 0.01M calcium chloride, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.70 Å R-free 0.288 |
| 2Q36 Actin Dimer Cross-linked between Residues 191 and 374 and complexed with Kabiramide C Deposited 2007-05-29 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
|
Not recorded | CA CALCIUM ION × 2 SO4 SULFATE ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 KAB KABIRAMIDE C × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；298 K；30% PEG4000, 0.1 M Tris, 0.2 M lithium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.50 Å R-free 0.223 |
| 2Q97 Complex of mammalian actin with toxofilin from toxoplasma gondii Deposited 2007-06-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 4.6；277 K；8% PEG 4000, 10% GLYCEROL, 0.1M SODIUM ACETATE, pH 4.6, VAPOR DIFFUSION, temperature 277K
|
Resolution 2.50 Å R-free 0.284 |
| 2Q97 Complex of mammalian actin with toxofilin from toxoplasma gondii Deposited 2007-06-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 4.6；277 K；8% PEG 4000, 10% GLYCEROL, 0.1M SODIUM ACETATE, pH 4.6, VAPOR DIFFUSION, temperature 277K
|
Resolution 2.50 Å R-free 0.284 |
| 2V51 Structure of MAL-RPEL1 complexed to actin Deposited 2008-10-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain B
1–377（377 aa）
Chain D
1–377（377 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 2 CA CALCIUM ION × 2 LAB LATRUNCULIN B × 2 PEG DI(HYDROXYETHYL)ETHER × 2 SCN THIOCYANATE ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.5；pH 6.5
|
Resolution 2.35 Å R-free 0.248 |
| 2V52 Structure of MAL-RPEL2 complexed to G-actin Deposited 2008-10-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
1–377（377 aa）
|
Not recorded | LAB LATRUNCULIN B × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GOL GLYCEROL × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.45 Å R-free 0.188 |
| 2VCP Crystal structure of N-Wasp VC domain in complex with skeletal actin Deposited 2007-09-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
Fragment：RESIDUES 3-377
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7；277 K；VAPOR DIFFUSION METHOD (4 C) PROTEIN SOLUTION: 0.18MM ACTIN, 0.36MM N-WASP PEPTIDE, 5MM TRIS.HCL PH7.0, ATP 0.2MM, CACL2 0.02MM, TCEP 20MM, NAN3 0.01%. RESERVOIR: 10.2% (V/V) TACSIMATE, 13.2%(W/V)PEG 8000, 100MM HEPES PH7.0
|
Resolution 3.20 Å R-free 0.331 |
| 2VCP Crystal structure of N-Wasp VC domain in complex with skeletal actin Deposited 2007-09-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
3–377（375 aa）
Fragment：RESIDUES 3-377
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7；277 K；VAPOR DIFFUSION METHOD (4 C) PROTEIN SOLUTION: 0.18MM ACTIN, 0.36MM N-WASP PEPTIDE, 5MM TRIS.HCL PH7.0, ATP 0.2MM, CACL2 0.02MM, TCEP 20MM, NAN3 0.01%. RESERVOIR: 10.2% (V/V) TACSIMATE, 13.2%(W/V)PEG 8000, 100MM HEPES PH7.0
|
Resolution 3.20 Å R-free 0.331 |
| 2VYP Rabbit-muscle G-actin in complex with myxobacterial rhizopodin Deposited 2008-07-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–377（377 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1 RH9 RHIZOPODIN × 1 HEZ HEXANE-1,6-DIOL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.6；100 MM MES PH 6.6 14% (W/W) PEG1500 12% (W/W) 1,6-HEXANEDIOL 100 MM CACL2 1 MM DTT 10 MM BETAINE-HYDROCHLORIDE
|
Resolution 2.35 Å R-free 0.241 |
| 2VYP Rabbit-muscle G-actin in complex with myxobacterial rhizopodin Deposited 2008-07-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–377（377 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.6；100 MM MES PH 6.6 14% (W/W) PEG1500 12% (W/W) 1,6-HEXANEDIOL 100 MM CACL2 1 MM DTT 10 MM BETAINE-HYDROCHLORIDE
|
Resolution 2.35 Å R-free 0.241 |
| 2W49 ISOMETRICALLY CONTRACTING INSECT ASYNCHRONOUS FLIGHT MUSCLE Deposited 2008-11-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 36 PDB declaration: 36-meric |
Chain D
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain E
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain F
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain G
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain H
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain I
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain J
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain K
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain L
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain M
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain N
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain O
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain P
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain Q
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain R
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain S
3–374（372 aa）
Fragment：RESIDUES 3-374
|
Not recorded | CA CALCIUM ION × 16 |
ELECTRON MICROSCOPY
cryo-EM buffer
20 MM MOPS BUFFER, 5 MM NAN3, AND MGCL2, ATP, CACL2, AND EGTA IN VARYING MILLIMOLAR CONCENTRATIONS；20 MM MOPS BUFFER, 5 MM NAN3, AND MGCL2, ATP, CACL2, AND EGTA IN VARYING MILLIMOLAR CONCENTRATIONS
cryo-EM vitrification conditions
Cryogen HELIUM；SMASH AGAINST A LIQUID HELIUM COOLED GOLD COATED COPPER MIRROR
|
Resolution 35.00 Å |
| 2W4U Isometrically contracting insect asynchronous flight muscle quick frozen after a length step Deposited 2008-12-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 36 PDB declaration: 36-meric |
Chain D
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain E
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain F
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain G
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain H
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain I
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain J
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain K
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain L
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain M
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain N
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain O
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain P
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain Q
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain R
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain S
3–374（372 aa）
Fragment：RESIDUES 3-374
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
20 MM MOPS BUFFER, 5 MM NAN3, AND MGCL2, ATP, CACL2, AND EGTA IN VARYING MILLIMOLAR CONCENTRATIONS；20 MM MOPS BUFFER, 5 MM NAN3, AND MGCL2, ATP, CACL2, AND EGTA IN VARYING MILLIMOLAR CONCENTRATIONS
cryo-EM vitrification conditions
Cryogen HELIUM；SMASH AGAINST A LIQUID HELIUM COOLED GOLD COATED COPPER MIRROR
|
Resolution 35.00 Å |
| 2Y83 Actin filament pointed end Deposited 2011-02-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain O
3–377（375 aa）
Chain P
3–377（375 aa）
Chain Q
3–377（375 aa）
Chain R
3–377（375 aa）
Chain S
3–377（375 aa）
Chain T
3–377（375 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 6 CA CALCIUM ION × 6 |
ELECTRON MICROSCOPY
cryo-EM buffer
50 MM NACL, 10 MM SODIUM PHOSPHATE BUFFER PH 7.4 3 MM MGCL2, 0.005% (W/V) NAN3, 0.7 MM DTT.；pH 7.4；50 MM NACL, 10 MM SODIUM PHOSPHATE BUFFER PH 7.4 3 MM MGCL2, 0.005% (W/V) NAN3, 0.7 MM DTT.
cryo-EM vitrification conditions
Cryogen ETHANE；VITRIFICATION 1 - CRYOGEN- ETHANE, HUMIDITY- 90%, TEMPERATURE- 4 DEGREES CELSIUS. METHOD- BLOT FOR 3 SECONDS BEFORE PLUNGING.
|
Resolution 22.90 Å |
| 2YJE Oligomeric assembly of actin bound to MRTF-A Deposited 2011-05-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
|
Not recorded | LAB LATRUNCULIN B × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8.25；0.1 M BTP PH 8.25, 20.5% PEG 3350, 0.2 M SODIUM NITRATE.
|
Resolution 3.10 Å R-free 0.280 |
| 2YJF Oligomeric assembly of actin bound to MRTF-A Deposited 2011-05-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
|
Not recorded | LAB LATRUNCULIN B × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5.3；pH 5.3
|
Resolution 3.50 Å R-free 0.272 |
| 2YJF Oligomeric assembly of actin bound to MRTF-A Deposited 2011-05-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
1–377（377 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5.3；pH 5.3
|
Resolution 3.50 Å R-free 0.272 |
| 2YJF Oligomeric assembly of actin bound to MRTF-A Deposited 2011-05-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain E
1–377（377 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5.3；pH 5.3
|
Resolution 3.50 Å R-free 0.272 |
| 2ZWH Model for the F-actin structure Deposited 2008-12-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 | FIBER DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 3.30 Å |
| 3B5U Actin filament model from extended form of acromsomal bundle in the Limulus sperm Deposited 2007-10-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 14 PDB declaration: tetradecameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
Chain H
1–377（377 aa）
Chain I
1–377（377 aa）
Chain J
1–377（377 aa）
Chain K
1–377（377 aa）
Chain L
1–377（377 aa）
Chain M
1–377（377 aa）
Chain N
1–377（377 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 7.4；see J Mol Biol, 221, 711-725 (1991)
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 9.50 Å |
| 3BUZ Crystal structure of ia-bTAD-actin complex Deposited 2008-01-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
3–377（375 aa）
|
Not recorded | TAD BETA-METHYLENE-THIAZOLE-4-CARBOXYAMIDE-ADENINE DINUCLEOTIDE × 1 CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 LAR LATRUNCULIN A × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；20% PEG1000, 0.1M MES(pH6.5), VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.81 Å R-free 0.298 |
| 3CJB Actin dimer cross-linked by V. cholerae MARTX toxin and complexed with Gelsolin-segment 1 Deposited 2008-03-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–377（377 aa）
|
Not recorded | CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；10% Peg MME 5000, 5% Tacsimate, 0.1 M Hepes, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.21 Å R-free 0.269 |
| 3CJC Actin dimer cross-linked by V. cholerae MARTX toxin and complexed with DNase I and Gelsolin-segment 1 Deposited 2008-03-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
1–377（377 aa）
|
Not recorded | CA CALCIUM ION × 2 SO4 SULFATE ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；1.5 M (NH4)SO4, 0.1 M Bis-Tris, 0.1 M NaCl, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.90 Å R-free 0.278 |
| 3DAW Structure of the actin-depolymerizing factor homology domain in complex with actin Deposited 2008-05-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–377（377 aa）
|
Not recorded | CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；10mM Tris pH 7.5, 50mM NaCl, 0.2mM ATP, 0.2mM DTT, 0.2mM CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.55 Å R-free 0.279 |
| 3FFK Crystal structure of human Gelsolin domains G1-G3 bound to Actin Deposited 2008-12-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
1–377（377 aa）
|
Not recorded | CA CALCIUM ION × 5 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；297 K；9% PEG 4000, 100 mM Sodium Acetate, 100 mM Calcium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 297K
|
Resolution 3.00 Å R-free 0.273 |
| 3FFK Crystal structure of human Gelsolin domains G1-G3 bound to Actin Deposited 2008-12-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain E
1–377（377 aa）
|
Not recorded | CA CALCIUM ION × 5 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；297 K；9% PEG 4000, 100 mM Sodium Acetate, 100 mM Calcium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 297K
|
Resolution 3.00 Å R-free 0.273 |
| 3G37 Cryo-EM structure of actin filament in the presence of phosphate Deposited 2009-02-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: dodecameric |
Chain O
3–377（375 aa）
Chain P
3–377（375 aa）
Chain Q
3–377（375 aa）
Chain R
3–377（375 aa）
Chain S
3–377（375 aa）
Chain T
3–377（375 aa）
Chain U
3–377（375 aa）
Chain V
3–377（375 aa）
Chain W
3–377（375 aa）
Chain X
3–377（375 aa）
Chain Y
3–377（375 aa）
Chain Z
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 12 PO4 PHOSPHATE ION × 36 MG MAGNESIUM ION × 72 |
ELECTRON MICROSCOPY
cryo-EM buffer
phosphate buffer；pH 7.4；phosphate buffer
cryo-EM vitrification conditions
77 K；Cryogen ETHANE
|
Resolution 6.00 Å |
| 3HBT The structure of native G-actin Deposited 2009-05-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 1 CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；295 K；2M ammonium sulfate, 100mM Tris-HCl, pH8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.70 Å R-free 0.257 |
| 3J4K Cryo-EM structures of the actin:tropomyosin filament reveal the mechanism for the transition from C- to M-state Deposited 2013-08-26 | Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
70 mM NaCl, 3 mM MgCl2, 0.2 mM EGTA, 5 mM NaH2PO4, 5 mM PIPES buffer；pH 7.5；70 mM NaCl, 3 mM MgCl2, 0.2 mM EGTA, 5 mM NaH2PO4, 5 mM PIPES buffer
cryo-EM vitrification conditions
3 second blot；Cryogen ETHANE；3 second blot before plunging into liquid ethane (FEI Vitrobot Mark IV)
|
Resolution 8.00 Å |
| 3J8A Structure of the F-actin-tropomyosin complex Deposited 2014-10-08 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
5 mM Tris-HCl, pH 7.5, 1 mM DTT, 100 mM KCl, 2 mM MgCl2；pH 7.5；5 mM Tris-HCl, pH 7.5, 1 mM DTT, 100 mM KCl, 2 mM MgCl2
cryo-EM vitrification conditions
Sample was applied to grid, incubated for 10 seconds, and manually blotted for 3 seconds from the backside with filter paper.；106 K；Cryogen ETHANE；Sample was applied to grid, incubated for 10 seconds, and manually blotted for 3 seconds from the backside with filter paper before plunging into liquid ethane (GATAN CRYOPLUNGE 3)
|
Resolution 3.70 Å R-free 0.271 |
| 3J8I Near-Atomic Resolution for One State of F-Actin Deposited 2014-11-06 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
Chain H
1–377（377 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM vitrification conditions
Cryogen ETHANE；Plunged into liquid ethane (FEI VITROBOT MARK IV)
|
Resolution 4.70 Å |
| 3J8J Tilted state of actin, T1 Deposited 2014-11-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 11 PDB declaration: undecameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
Chain H
1–377（377 aa）
Chain I
1–377（377 aa）
Chain J
1–377（377 aa）
Chain K
1–377（377 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM vitrification conditions
Cryogen ETHANE；Plunged into liquid ethane
|
Resolution 12.00 Å |
| 3J8K Tilted state of actin, T2 Deposited 2014-11-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
Chain H
1–377（377 aa）
Chain I
1–377（377 aa）
Chain J
1–377（377 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 12.00 Å |
| 3JBI MDFF model of the vinculin tail domain bound to F-actin Deposited 2015-09-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
|
Not recorded | MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
50 mM KCl, 1 mM MgCl2, 1 mM EGTA, 10 mM imidazole；pH 7；50 mM KCl, 1 mM MgCl2, 1 mM EGTA, 10 mM imidazole
cryo-EM vitrification conditions
3 microliters of 0.3 micromolar actin was applied to the grid and incubated for 60 seconds at 25 degrees C. 3 microliters of 10 micromolar Vt was then applied and incubated for 60 seconds. 3 microliters of solution was removed, then an additional 3 microliters of Vt applied. After 60 seconds, 3 microliters of solution was removed, then the grid was blotted for 2 seconds before plunging.；Cryogen ETHANE；3 microliters of 0.3 micromolar actin was applied to the grid and incubated for 60 seconds at 25 degrees C. 3 microliters of 10 micromolar Vt was then applied and incubated for 60 seconds. 3 microliters of solution was removed, then an additional 3 microliters of Vt applied. After 60 seconds, 3 microliters of solution was removed, then the grid was blotted for 2 seconds before plunging into liquid ethane (LEICA EM GP).
|
Resolution 8.50 Å |
| 3JBJ Cryo-EM reconstruction of F-actin Deposited 2015-09-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
|
Not recorded | MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
50 mM KCl, 1 mM MgCl2, 1 mM EGTA, 10 mM imidazole；pH 7；50 mM KCl, 1 mM MgCl2, 1 mM EGTA, 10 mM imidazole
cryo-EM vitrification conditions
3 microliters of 0.3 micromolar actin was applied to the grid and incubated for 60 seconds at 25 degrees C. The grid was blotted for 2 seconds before plunging.；Cryogen ETHANE；3 microliters of 0.3 micromolar actin was applied to the grid and incubated for 60 seconds at 25 degrees C. The grid was blotted for 2 seconds before plunging into liquid ethane (LEICA EM GP).
|
Resolution 7.60 Å |
| 3JBK Cryo-EM reconstruction of the metavinculin-actin interface Deposited 2015-09-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
|
Not recorded | MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
50 mM KCl, 1 mM MgCl2, 1 mM EGTA, 10 mM imidazole；pH 7；50 mM KCl, 1 mM MgCl2, 1 mM EGTA, 10 mM imidazole
cryo-EM vitrification conditions
3 microliters of 0.3 micromolar actin was applied to the grid and incubated for 60 seconds at 25 degrees C. 3 microliters of 10 micromolar MVt was then applied and incubated for 60 seconds. 3 microliters of solution was removed, then an additional 3 microliters of MVt applied. After 60 seconds, 3 microliters of solution was removed, then the grid was blotted for 2 seconds before plunging.；Cryogen ETHANE；3 microliters of 0.3 micromolar actin was applied to the grid and incubated for 60 seconds at 25 degrees C. 3 microliters of 10 micromolar MVt was then applied and incubated for 60 seconds. 3 microliters of solution was removed, then an additional 3 microliters of MVt applied. After 60 seconds, 3 microliters of solution was removed, then the grid was blotted for 2 seconds before plunging into liquid ethane (LEICA EM GP).
|
Resolution 8.20 Å |
| 3M1F Crosslinked complex of actin with first W domain of Vibrio parahaemolyticus VopL Deposited 2010-03-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.1；298 K；0.2 N Lithium Nitrate, 20% polyethylene glycol 3350, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
Resolution 2.89 Å R-free 0.265 |
| 3M3N Structure of a Longitudinal Actin Dimer Assembled by Tandem W Domains Deposited 2010-03-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ATP ADENOSINE-5'-TRIPHOSPHATE × 2 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 10；300 K；100 mM CAPS pH 10.0, and 24% PEG 3350, 100 mM RbCl, VAPOR DIFFUSION, HANGING DROP, temperature 300.0K
|
Resolution 7.00 Å |
| 3M6G Crystal structure of actin in complex with lobophorolide Deposited 2010-03-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
3–373（371 aa）
Fragment：UNP residues 3-373
Chain B
3–373（371 aa）
Fragment：UNP residues 3-373
|
Not recorded | MG MAGNESIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 LO3 (1S,3S,4S,5S,7R,8S,9R,12E,14E,16R,17R,19R)-16-hydroxy-9-{(1S,2S,3S)-2-hydroxy-5-[(2S,4R,6S)-4-methoxy-6-methyltetrahydro-2H-pyran-2-yl]-1,3-dimethylpentyl}-3,5,7,17-tetramethoxy-8,14-dimethyl-11H-spiro[10,23-dioxabicyclo[17.3.1]tricosa-12,14,20-triene-4,2'-oxiran]-11-one × 2 CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；0.1M MES pH 6.0, 6% methyl
ether poly(ethylene glycol) 5000, 0.1M CaCl2, 1mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.00 Å R-free 0.227 |
| 3M6G Crystal structure of actin in complex with lobophorolide Deposited 2010-03-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–373（371 aa）
Fragment：UNP residues 3-373
|
Not recorded | MG MAGNESIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 LO3 (1S,3S,4S,5S,7R,8S,9R,12E,14E,16R,17R,19R)-16-hydroxy-9-{(1S,2S,3S)-2-hydroxy-5-[(2S,4R,6S)-4-methoxy-6-methyltetrahydro-2H-pyran-2-yl]-1,3-dimethylpentyl}-3,5,7,17-tetramethoxy-8,14-dimethyl-11H-spiro[10,23-dioxabicyclo[17.3.1]tricosa-12,14,20-triene-4,2'-oxiran]-11-one × 1 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；0.1M MES pH 6.0, 6% methyl
ether poly(ethylene glycol) 5000, 0.1M CaCl2, 1mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.00 Å R-free 0.227 |
| 3M6G Crystal structure of actin in complex with lobophorolide Deposited 2010-03-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
3–373（371 aa）
Fragment：UNP residues 3-373
|
Not recorded | MG MAGNESIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 LO3 (1S,3S,4S,5S,7R,8S,9R,12E,14E,16R,17R,19R)-16-hydroxy-9-{(1S,2S,3S)-2-hydroxy-5-[(2S,4R,6S)-4-methoxy-6-methyltetrahydro-2H-pyran-2-yl]-1,3-dimethylpentyl}-3,5,7,17-tetramethoxy-8,14-dimethyl-11H-spiro[10,23-dioxabicyclo[17.3.1]tricosa-12,14,20-triene-4,2'-oxiran]-11-one × 1 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；0.1M MES pH 6.0, 6% methyl
ether poly(ethylene glycol) 5000, 0.1M CaCl2, 1mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.00 Å R-free 0.227 |
| 3MFP Atomic model of F-actin based on a 6.6 angstrom resolution cryoEM map Deposited 2010-04-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.60 Å |
| 3MFP Atomic model of F-actin based on a 6.6 angstrom resolution cryoEM map Deposited 2010-04-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.60 Å |
| 3MFP Atomic model of F-actin based on a 6.6 angstrom resolution cryoEM map Deposited 2010-04-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.60 Å |
| 3MN5 Structures of actin-bound WH2 domains of Spire and the implication for filament nucleation Deposited 2010-04-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
|
Not recorded | CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 LAB LATRUNCULIN B × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.9；277 K；0.2 Magnesium formate pH 5.9
20% PEG 3350
, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 1.50 Å R-free 0.216 |
| 3SJH Crystal Structure of a chimera containing the N-terminal domain (residues 8-29) of drosophila Ciboulot and the C-terminal domain (residues 18-44) of bovine Thymosin-beta4, bound to G-actin-ATP-Latrunculin A Deposited 2011-06-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
Fragment：UNP residues 3-377
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 LAR LATRUNCULIN A × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
hanging drop；pH 6.5；298 K；22% PEG3350, 0.2M MgAcetate pH6.5, 0.45M Guanidine HCl, 1% Dioxane, hanging drop, temperature 298K
|
Resolution 1.75 Å R-free 0.199 |
| 3TU5 Actin complex with Gelsolin Segment 1 fused to Cobl segment Deposited 2011-09-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–377（377 aa）
|
Not recorded | CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；298 K；10% (w/v) polyethyleneglycol 20000, 2% (v/v) dioxane, and 0.1M bicine, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.00 Å R-free 0.204 |
| 3U8X Crystal Structure of a chimera containing the N-terminal domain (residues 8-29) of drosophila Ciboulot and the C-terminal domain (residues 18-44) of bovine Thymosin-beta4, bound to G-actin-ATP Deposited 2011-10-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
hanging drop；pH 6.5；298 K；20% PEG3350, 0.2M MgAcetate pH6.5, 0.45M Guanidine HCl, 1% Dioxane, hanging drop, temperature 298K
|
Resolution 2.00 Å R-free 0.238 |
| 3U8X Crystal Structure of a chimera containing the N-terminal domain (residues 8-29) of drosophila Ciboulot and the C-terminal domain (residues 18-44) of bovine Thymosin-beta4, bound to G-actin-ATP Deposited 2011-10-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
3–377（375 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
hanging drop；pH 6.5；298 K；20% PEG3350, 0.2M MgAcetate pH6.5, 0.45M Guanidine HCl, 1% Dioxane, hanging drop, temperature 298K
|
Resolution 2.00 Å R-free 0.238 |
| 3U9Z Crystal structure between actin and a protein construct containing the first beta-thymosin domain of drosophila ciboulot (residues 2-58) with the three mutations N26D/Q27K/D28S Deposited 2011-10-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
hanging drop；pH 6.5；298 K；18% PEG3350, 0.05M NaAcetate pH4.7, 0.1M MgAcetate pH6.5, 0.32M Guanidine HCl, 0.8% Dioxane, hanging drop, temperature 298K
|
Resolution 2.09 Å R-free 0.227 |
| 3UE5 ECP-cleaved Actin in complex with Spir domain D Deposited 2011-10-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain A
3–377（375 aa）
|
Not recorded | CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 CL CHLORIDE ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；298 K；Tris pH 8.5, magnesium chloride, PEG-8000, vapor diffusion, hanging drop, temperature 298K
|
Resolution 2.76 Å R-free 0.240 |
| 3UE5 ECP-cleaved Actin in complex with Spir domain D Deposited 2011-10-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain A
3–377（375 aa）
|
Not recorded | CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 CL CHLORIDE ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；298 K；Tris pH 8.5, magnesium chloride, PEG-8000, vapor diffusion, hanging drop, temperature 298K
|
Resolution 2.76 Å R-free 0.240 |
| 3UE5 ECP-cleaved Actin in complex with Spir domain D Deposited 2011-10-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain A
3–377（375 aa）
|
Not recorded | CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 CL CHLORIDE ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；298 K；Tris pH 8.5, magnesium chloride, PEG-8000, vapor diffusion, hanging drop, temperature 298K
|
Resolution 2.76 Å R-free 0.240 |
| 4A7F Structure of the Actin-Tropomyosin-Myosin Complex (rigor ATM 3) Deposited 2011-11-14 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
Chain F
3–377（375 aa）
Chain I
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 CA CALCIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
5 MM HEPES-OH, 100 MM KCL, 2 MM MGCL2, 50 MM GLUTAMINE, 50 MM ARGININE；pH 7.2；5 MM HEPES-OH, 100 MM KCL, 2 MM MGCL2, 50 MM GLUTAMINE, 50 MM ARGININE
cryo-EM vitrification conditions
Cryogen ETHANE；LIQUID ETHANE
|
Resolution 7.70 Å |
| 4A7H Structure of the Actin-Tropomyosin-Myosin Complex (rigor ATM 2) Deposited 2011-11-14 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
Chain F
3–377（375 aa）
Chain G
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 CA CALCIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
5MM TRIS, 100MM KCL, 2MM MGCL2, 50MM GLUTAMINE, 50MM ARGININ；pH 7.2；5MM TRIS, 100MM KCL, 2MM MGCL2, 50MM GLUTAMINE, 50MM ARGININ
cryo-EM vitrification conditions
Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 90, TEMPERATURE- 101, INSTRUMENT- GATAN CRYOPLUNGE 3, METHOD- MANUAL BLOTTING FOR APPROXIMATELY 15 SECONDS,
|
Resolution 7.80 Å |
| 4A7L Structure of the Actin-Tropomyosin-Myosin Complex (rigor ATM 1) Deposited 2011-11-14 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
Chain F
3–377（375 aa）
Chain I
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 CA CALCIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
5MM TRIS, 100MM KCL, 2MM MGCL2, 50MM GLUTAMINE, 50MM ARGININ；pH 7.2；5MM TRIS, 100MM KCL, 2MM MGCL2, 50MM GLUTAMINE, 50MM ARGININ
cryo-EM vitrification conditions
Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 90, TEMPERATURE- 101, INSTRUMENT- GATAN CRYOPLUNGE 3, METHOD- MANUAL BLOTTING FOR APPROXIMATELY 15 SECONDS,
|
Resolution 8.10 Å |
| 4A7N Structure of bare F-actin filaments obtained from the same sample as the Actin-Tropomyosin-Myosin Complex Deposited 2011-11-14 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 CA CALCIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
5MM TRIS, 100MM KCL, 2MM MGCL2, 50MM GLUTAMINE, 50MM ARGININE；pH 7.2；5MM TRIS, 100MM KCL, 2MM MGCL2, 50MM GLUTAMINE, 50MM ARGININE
cryo-EM vitrification conditions
Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 90, TEMPERATURE- 101, INSTRUMENT- GATAN CRYOPLUNGE 3, METHOD- MANUAL BLOTTING FOR APPROXIMATELY 15 SECONDS,
|
Resolution 8.90 Å |
| 4B1V Structure of the Phactr1 RPEL-N domain bound to G-actin Deposited 2012-07-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
2–377（376 aa）
Fragment：RESIDUES 2-377
|
Not recorded | EDO 1,2-ETHANEDIOL × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 LAB LATRUNCULIN B × 1 MG MAGNESIUM ION × 1 GOL GLYCEROL × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.75 Å R-free 0.221 |
| 4B1V Structure of the Phactr1 RPEL-N domain bound to G-actin Deposited 2012-07-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
2–377（376 aa）
Fragment：RESIDUES 2-377
|
Not recorded | EDO 1,2-ETHANEDIOL × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 LAB LATRUNCULIN B × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.75 Å R-free 0.221 |
| 4B1W Structure of the Phactr1 RPEL-2 domain bound to actin Deposited 2012-07-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
2–377（376 aa）
|
Not recorded | LAB LATRUNCULIN B × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SO4 SULFATE ION × 1 GOL GLYCEROL × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.95 Å R-free 0.213 |
| 4B1X Structure of the Phactr1 RPEL-2 bound to G-actin Deposited 2012-07-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
2–377（376 aa）
|
Not recorded | LAB LATRUNCULIN B × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.80 Å R-free 0.213 |
| 4B1Y Structure of the Phactr1 RPEL-3 bound to G-actin Deposited 2012-07-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
2–377（376 aa）
|
Not recorded | LAB LATRUNCULIN B × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 1PE PENTAETHYLENE GLYCOL × 1 GOL GLYCEROL × 1 PEG DI(HYDROXYETHYL)ETHER × 3 P6G HEXAETHYLENE GLYCOL × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.29 Å R-free 0.174 |
| 4B1Z Structure of the Phactr1 RPEL domain bound to G-actin Deposited 2012-07-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
2–377（376 aa）
Chain B
2–377（376 aa）
Chain C
2–377（376 aa）
Chain D
2–377（376 aa）
Chain E
2–377（376 aa）
Chain F
2–377（376 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 GOL GLYCEROL × 8 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 3.30 Å R-free 0.236 |
| 4EAH Crystal structure of the formin homology 2 domain of FMNL3 bound to actin Deposited 2012-03-22 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain D
1–377（377 aa）
Chain G
1–377（377 aa）
|
Not recorded | ACT ACETATE ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；11% PEG 10000, 0.2 M magnesium acetate, 0.1 M MES, pH 6.5, Silver Bullet 33 (0.20% w/v D-(+)-Maltose monohydrate, 0.20% w/v D-(+)-Melibiose monohydrate, 0.20% w/v D-(+)-Raffinose pentahydrate, 0.20% w/v D-(+)-Trehalose dihydrate, 0.20% w/v Stachyose hydrate, 0.02 M HEPES sodium pH 6.8), Silver Bullet 70 (0.2% w/v Anthrone, 0.2% w/v Benzidine, 0.2% w/v N-(2-Acetamido)-2-aminoethanesulfonic acid, 0.2% w/v Phenylurea, 0.2% w/v -Alanine, 0.02 M HEPES sodium pH 6.8), VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.40 Å R-free 0.277 |
| 4EAH Crystal structure of the formin homology 2 domain of FMNL3 bound to actin Deposited 2012-03-22 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain F
1–377（377 aa）
Chain H
1–377（377 aa）
|
Not recorded | ACT ACETATE ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；11% PEG 10000, 0.2 M magnesium acetate, 0.1 M MES, pH 6.5, Silver Bullet 33 (0.20% w/v D-(+)-Maltose monohydrate, 0.20% w/v D-(+)-Melibiose monohydrate, 0.20% w/v D-(+)-Raffinose pentahydrate, 0.20% w/v D-(+)-Trehalose dihydrate, 0.20% w/v Stachyose hydrate, 0.02 M HEPES sodium pH 6.8), Silver Bullet 70 (0.2% w/v Anthrone, 0.2% w/v Benzidine, 0.2% w/v N-(2-Acetamido)-2-aminoethanesulfonic acid, 0.2% w/v Phenylurea, 0.2% w/v -Alanine, 0.02 M HEPES sodium pH 6.8), VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.40 Å R-free 0.277 |
| 4GY2 Crystal structure of apo-Ia-actin complex Deposited 2012-09-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
3–377（375 aa）
|
Not recorded | PO4 PHOSPHATE ION × 1 CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 LAR LATRUNCULIN A × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.13 K；18% PEG 1500, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.13K
|
Resolution 2.71 Å R-free 0.257 |
| 4H03 Crystal structure of NAD+-Ia-actin complex Deposited 2012-09-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 PO4 PHOSPHATE ION × 1 EDO 1,2-ETHANEDIOL × 48 CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 LAR LATRUNCULIN A × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.13 K；18% PEG1500, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.13K
|
Resolution 1.75 Å R-free 0.233 |
| 4H0T Crystal structure of Ia-ADPR-actin complex Deposited 2012-09-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
3–377（375 aa）
|
Not recorded | PO4 PHOSPHATE ION × 1 EDO 1,2-ETHANEDIOL × 18 AR6 [(2R,3S,4R,5R)-5-(6-AMINOPURIN-9-YL)-3,4-DIHYDROXY-OXOLAN-2-YL]METHYL[HYDROXY-[[(2R,3S,4R,5S)-3,4,5-TRIHYDROXYOXOLAN-2-YL]METHOXY]PHOSPHORYL] HYDROGEN PHOSPHATE × 1 CA CALCIUM ION × 1 LAR LATRUNCULIN A × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.13 K；18% PEG1500, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.13K
|
Resolution 2.20 Å R-free 0.240 |
| 4H0V Crystal structure of NAD+-Ia(E378S)-actin complex Deposited 2012-09-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
3–377（375 aa）
|
Not recorded | NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 PO4 PHOSPHATE ION × 1 EDO 1,2-ETHANEDIOL × 39 CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 LAR LATRUNCULIN A × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.13 K；18% PEG1500, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.13K
|
Resolution 2.03 Å R-free 0.234 |
| 4H0X Crystal structure of NAD+-Ia(E380A)-actin complex Deposited 2012-09-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
3–377（375 aa）
|
Not recorded | NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 PO4 PHOSPHATE ION × 1 EDO 1,2-ETHANEDIOL × 22 CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 LAR LATRUNCULIN A × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.13 K；18% PEG1500, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.13K
|
Resolution 2.33 Å R-free 0.251 |
| 4H0Y Crystal structure of NAD+-Ia(E380S)-actin complex Deposited 2012-09-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
3–377（375 aa）
|
Not recorded | NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 PO4 PHOSPHATE ION × 1 EDO 1,2-ETHANEDIOL × 52 CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 LAR LATRUNCULIN A × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.13 K；18% PEG1500, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.13K
|
Resolution 1.94 Å R-free 0.233 |
| 4K41 Crystal structure of actin in complex with marine macrolide kabiramide C Deposited 2013-04-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–377（375 aa）
|
Not recorded | CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 KAB KABIRAMIDE C × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；298 K；0.1 M MES pH 5.5, 0.1 M CaCl2, 12% 1,6-Hexanediol and 17% Polyethylene glycol 1500, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.40 Å R-free 0.195 |
| 4K42 Crystal structure of actin in complex with synthetic AplC tail analogue SF01 [(3R,4S,5R,6S,10R,11R,12R)-11-(acetyloxy)-1-(benzyloxy)-14-[formyl(methyl)amino]-5-hydroxy-4,6,10,12-tetramethyl-9-oxotetradecan-3-yl propanoate] Deposited 2013-04-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–377（375 aa）
|
Not recorded | CA CALCIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NWM (3R,4S,5R,6S,10R,11R,12R)-11-(acetyloxy)-1-(benzyloxy)-14-[formyl(methyl)amino]-5-hydroxy-4,6,10,12-tetramethyl-9-oxotetradecan-3-yl propanoate × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；298 K；0.1 M BIS-TRIS pH 5.5, 25% Polyethylene glycol 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.90 Å R-free 0.271 |
| 4K42 Crystal structure of actin in complex with synthetic AplC tail analogue SF01 [(3R,4S,5R,6S,10R,11R,12R)-11-(acetyloxy)-1-(benzyloxy)-14-[formyl(methyl)amino]-5-hydroxy-4,6,10,12-tetramethyl-9-oxotetradecan-3-yl propanoate] Deposited 2013-04-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
3–377（375 aa）
|
Not recorded | CA CALCIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NWM (3R,4S,5R,6S,10R,11R,12R)-11-(acetyloxy)-1-(benzyloxy)-14-[formyl(methyl)amino]-5-hydroxy-4,6,10,12-tetramethyl-9-oxotetradecan-3-yl propanoate × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；298 K；0.1 M BIS-TRIS pH 5.5, 25% Polyethylene glycol 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.90 Å R-free 0.271 |
| 4K42 Crystal structure of actin in complex with synthetic AplC tail analogue SF01 [(3R,4S,5R,6S,10R,11R,12R)-11-(acetyloxy)-1-(benzyloxy)-14-[formyl(methyl)amino]-5-hydroxy-4,6,10,12-tetramethyl-9-oxotetradecan-3-yl propanoate] Deposited 2013-04-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
3–377（375 aa）
|
Not recorded | CA CALCIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NWM (3R,4S,5R,6S,10R,11R,12R)-11-(acetyloxy)-1-(benzyloxy)-14-[formyl(methyl)amino]-5-hydroxy-4,6,10,12-tetramethyl-9-oxotetradecan-3-yl propanoate × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；298 K；0.1 M BIS-TRIS pH 5.5, 25% Polyethylene glycol 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.90 Å R-free 0.271 |
| 4K42 Crystal structure of actin in complex with synthetic AplC tail analogue SF01 [(3R,4S,5R,6S,10R,11R,12R)-11-(acetyloxy)-1-(benzyloxy)-14-[formyl(methyl)amino]-5-hydroxy-4,6,10,12-tetramethyl-9-oxotetradecan-3-yl propanoate] Deposited 2013-04-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
3–377（375 aa）
|
Not recorded | CA CALCIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NWM (3R,4S,5R,6S,10R,11R,12R)-11-(acetyloxy)-1-(benzyloxy)-14-[formyl(methyl)amino]-5-hydroxy-4,6,10,12-tetramethyl-9-oxotetradecan-3-yl propanoate × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；298 K；0.1 M BIS-TRIS pH 5.5, 25% Polyethylene glycol 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.90 Å R-free 0.271 |
| 4K43 Crystal structure of actin in complex with synthetic AplC tail analogue GC04 [N-{(1E,4R,5R,7E,9S,10S,11S)-4,10-dimethoxy-11-[(2S,4S,5S)-2-(4-methoxyphenyl)-5-methyl-1,3-dioxan-4-yl]-5,9-dimethyl-6-oxododeca-1,7-dien-1-yl}-N-methylformamide] Deposited 2013-04-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–377（375 aa）
|
Not recorded | CA CALCIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 1PO N-{(1E,4R,5R,7E,9S,10S,11S)-4,10-dimethoxy-11-[(2S,4S,5S)-2-(4-methoxyphenyl)-5-methyl-1,3-dioxan-4-yl]-5,9-dimethyl-6-oxododeca-1,7-dien-1-yl}-N-methylformamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；298 K；0.1 M BIS-TRIS pH 5.5, 25% Polyethylene glycol 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.90 Å R-free 0.277 |
| 4K43 Crystal structure of actin in complex with synthetic AplC tail analogue GC04 [N-{(1E,4R,5R,7E,9S,10S,11S)-4,10-dimethoxy-11-[(2S,4S,5S)-2-(4-methoxyphenyl)-5-methyl-1,3-dioxan-4-yl]-5,9-dimethyl-6-oxododeca-1,7-dien-1-yl}-N-methylformamide] Deposited 2013-04-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
3–377（375 aa）
|
Not recorded | CA CALCIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 1PO N-{(1E,4R,5R,7E,9S,10S,11S)-4,10-dimethoxy-11-[(2S,4S,5S)-2-(4-methoxyphenyl)-5-methyl-1,3-dioxan-4-yl]-5,9-dimethyl-6-oxododeca-1,7-dien-1-yl}-N-methylformamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；298 K；0.1 M BIS-TRIS pH 5.5, 25% Polyethylene glycol 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.90 Å R-free 0.277 |
| 4PKG Complex of ATP-actin With the N-terminal Actin-Binding Domain of Tropomodulin Deposited 2014-05-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；0.18 M sodium fluoride, 11% w/v PEG3350, 1% v/v PEG1000, 1% v/v PEG400
|
Resolution 1.80 Å R-free 0.187 |
| 4PKH Complex of ADP-actin With the N-terminal Actin-Binding Domain of Tropomodulin Deposited 2014-05-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–377（377 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 1 CA CALCIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；0.18 M sodium fluoride, 11% w/v PEG3350, 1% v/v PEG1000, 1% v/v PEG400
|
Resolution 2.15 Å R-free 0.326 |
| 4PKH Complex of ADP-actin With the N-terminal Actin-Binding Domain of Tropomodulin Deposited 2014-05-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric |
Chain D
1–377（377 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 1 CA CALCIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；0.18 M sodium fluoride, 11% w/v PEG3350, 1% v/v PEG1000, 1% v/v PEG400
|
Resolution 2.15 Å R-free 0.326 |
| 4PKH Complex of ADP-actin With the N-terminal Actin-Binding Domain of Tropomodulin Deposited 2014-05-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric |
Chain F
1–377（377 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 1 CA CALCIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；0.18 M sodium fluoride, 11% w/v PEG3350, 1% v/v PEG1000, 1% v/v PEG400
|
Resolution 2.15 Å R-free 0.326 |
| 4PKH Complex of ADP-actin With the N-terminal Actin-Binding Domain of Tropomodulin Deposited 2014-05-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric |
Chain I
1–377（377 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 1 CA CALCIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；0.18 M sodium fluoride, 11% w/v PEG3350, 1% v/v PEG1000, 1% v/v PEG400
|
Resolution 2.15 Å R-free 0.326 |
| 4PKI Complex of ATP-actin With the C-terminal Actin-Binding Domain of Tropomodulin Deposited 2014-05-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；0.25 M sodium chloride, 12% w/v PEG3350
|
Resolution 2.30 Å R-free 0.201 |
| 4PL8 Structure of rabbit skeletal muscle actin in complex with a hybrid peptide comprising thymosin beta4 and the lysine-rich region of Cordon-Bleu Deposited 2014-05-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ATP ADENOSINE-5'-TRIPHOSPHATE × 2 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 3.5；288 K；0.1 M citric acid, 15% (w/v) PEG 3350
|
Resolution 2.00 Å R-free 0.207 |
| 4V0U The crystal structure of ternary PP1G-PPP1R15B and G-actin complex Deposited 2014-09-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 10 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain M
3–377（375 aa）
Fragment：RESIDUES 3-377
|
Not recorded | LAB LATRUNCULIN B × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7；0.2M CACL2, 0.1 M HEPES, PH7.0, 20% PEG6000
|
Resolution 7.88 Å R-free 0.400 |
| 4V0U The crystal structure of ternary PP1G-PPP1R15B and G-actin complex Deposited 2014-09-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 6 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–377（375 aa）
Fragment：RESIDUES 3-377
|
Not recorded | LAB LATRUNCULIN B × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7；0.2M CACL2, 0.1 M HEPES, PH7.0, 20% PEG6000
|
Resolution 7.88 Å R-free 0.400 |
| 4V0U The crystal structure of ternary PP1G-PPP1R15B and G-actin complex Deposited 2014-09-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 7 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
3–377（375 aa）
Fragment：RESIDUES 3-377
|
Not recorded | LAB LATRUNCULIN B × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7；0.2M CACL2, 0.1 M HEPES, PH7.0, 20% PEG6000
|
Resolution 7.88 Å R-free 0.400 |
| 4V0U The crystal structure of ternary PP1G-PPP1R15B and G-actin complex Deposited 2014-09-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 8 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
3–377（375 aa）
Fragment：RESIDUES 3-377
|
Not recorded | LAB LATRUNCULIN B × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7；0.2M CACL2, 0.1 M HEPES, PH7.0, 20% PEG6000
|
Resolution 7.88 Å R-free 0.400 |
| 4V0U The crystal structure of ternary PP1G-PPP1R15B and G-actin complex Deposited 2014-09-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 9 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain L
3–377（375 aa）
Fragment：RESIDUES 3-377
|
Not recorded | LAB LATRUNCULIN B × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7；0.2M CACL2, 0.1 M HEPES, PH7.0, 20% PEG6000
|
Resolution 7.88 Å R-free 0.400 |
| 4WYB Structure of the Bud6 flank domain in complex with actin Deposited 2014-11-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–377（377 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；3.5M sodium formate, 0.1M CaCl2, and 5 mM TCEP
|
Resolution 3.49 Å R-free 0.258 |
| 4WYB Structure of the Bud6 flank domain in complex with actin Deposited 2014-11-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 10 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain S
1–377（377 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；3.5M sodium formate, 0.1M CaCl2, and 5 mM TCEP
|
Resolution 3.49 Å R-free 0.258 |
| 4WYB Structure of the Bud6 flank domain in complex with actin Deposited 2014-11-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 11 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain U
1–377（377 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；3.5M sodium formate, 0.1M CaCl2, and 5 mM TCEP
|
Resolution 3.49 Å R-free 0.258 |
| 4WYB Structure of the Bud6 flank domain in complex with actin Deposited 2014-11-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 12 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain X
1–377（377 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；3.5M sodium formate, 0.1M CaCl2, and 5 mM TCEP
|
Resolution 3.49 Å R-free 0.258 |
| 4WYB Structure of the Bud6 flank domain in complex with actin Deposited 2014-11-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
1–377（377 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；3.5M sodium formate, 0.1M CaCl2, and 5 mM TCEP
|
Resolution 3.49 Å R-free 0.258 |
| 4WYB Structure of the Bud6 flank domain in complex with actin Deposited 2014-11-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain E
1–377（377 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；3.5M sodium formate, 0.1M CaCl2, and 5 mM TCEP
|
Resolution 3.49 Å R-free 0.258 |
| 4WYB Structure of the Bud6 flank domain in complex with actin Deposited 2014-11-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain G
1–377（377 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；3.5M sodium formate, 0.1M CaCl2, and 5 mM TCEP
|
Resolution 3.49 Å R-free 0.258 |
| 4WYB Structure of the Bud6 flank domain in complex with actin Deposited 2014-11-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 5 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain I
1–377（377 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；3.5M sodium formate, 0.1M CaCl2, and 5 mM TCEP
|
Resolution 3.49 Å R-free 0.258 |
| 4WYB Structure of the Bud6 flank domain in complex with actin Deposited 2014-11-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 6 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain K
1–377（377 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；3.5M sodium formate, 0.1M CaCl2, and 5 mM TCEP
|
Resolution 3.49 Å R-free 0.258 |
| 4WYB Structure of the Bud6 flank domain in complex with actin Deposited 2014-11-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 7 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain M
1–377（377 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；3.5M sodium formate, 0.1M CaCl2, and 5 mM TCEP
|
Resolution 3.49 Å R-free 0.258 |
| 4WYB Structure of the Bud6 flank domain in complex with actin Deposited 2014-11-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 8 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain O
1–377（377 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；3.5M sodium formate, 0.1M CaCl2, and 5 mM TCEP
|
Resolution 3.49 Å R-free 0.258 |
| 4WYB Structure of the Bud6 flank domain in complex with actin Deposited 2014-11-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 9 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain Q
1–377（377 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；3.5M sodium formate, 0.1M CaCl2, and 5 mM TCEP
|
Resolution 3.49 Å R-free 0.258 |
| 4Z94 Actin Complex With a Chimera of Tropomodulin-1 and Leiomodin-1 Actin-Binding Site 2 Deposited 2015-04-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.8；291.5 K；20% PEG3350, 200 mM lithium sulfate, 100 mM Tris, pH 8.8, 15% glycerol
|
Resolution 2.40 Å R-free 0.243 |
| 5H53 The structure of rabbit skeletal muscle actomyosin rigor complex at 5.2 angstrom. Deposited 2016-11-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric |
Chain D
3–377（375 aa）
Fragment：UNP residues 3-377
Chain E
3–377（375 aa）
Fragment：UNP residues 3-377
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.20 Å |
| 5JLF Structure of the F-actin-tropomyosin complex (Reprocessed) Deposited 2016-04-27 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；5 mM Tris-HCl pH 7.5, 1 mM DTT, 100 mM KCl, and 2 mM MgCl2
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was applied to a glow-discharged holey carbon grid, incubated for 10 s and manually blotted for 3 s from the backside with filter paper.
|
Resolution 3.60 Å |
| 5KG8 Rigor myosin X co-complexed with an actin filament Deposited 2016-06-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 9.10 Å |
| 5MVA Structure of the thin filament at high calcium concentration Deposited 2017-01-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 23 PDB declaration: 23-meric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
Chain F
3–377（375 aa）
Chain G
3–377（375 aa）
Chain H
3–377（375 aa）
Chain I
3–377（375 aa）
Chain J
3–377（375 aa）
Chain K
3–377（375 aa）
Chain L
3–377（375 aa）
Chain M
3–377（375 aa）
Chain N
3–377（375 aa）
Chain O
3–377（375 aa）
Chain P
3–377（375 aa）
Chain Q
3–377（375 aa）
Chain R
3–377（375 aa）
Chain S
3–377（375 aa）
Chain T
3–377（375 aa）
Chain U
3–377（375 aa）
Chain V
3–377（375 aa）
Chain W
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 23 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
|
Resolution 27.70 Å |
| 5MVY Thin Filament at low calcium concentration Deposited 2017-01-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 23 PDB declaration: 23-meric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
Chain F
3–377（375 aa）
Chain G
3–377（375 aa）
Chain H
3–377（375 aa）
Chain I
3–377（375 aa）
Chain J
3–377（375 aa）
Chain K
3–377（375 aa）
Chain L
3–377（375 aa）
Chain M
3–377（375 aa）
Chain N
3–377（375 aa）
Chain O
3–377（375 aa）
Chain P
3–377（375 aa）
Chain Q
3–377（375 aa）
Chain R
3–377（375 aa）
Chain S
3–377（375 aa）
Chain T
3–377（375 aa）
Chain U
3–377（375 aa）
Chain V
3–377（375 aa）
Chain W
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 23 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
|
Resolution 28.40 Å |
| 5ONV Cryo-EM structure of F-actin in complex with ADP Deposited 2017-08-04 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；5 mM HEPES pH 7.5, 0.1 M KCl, 2 mM MgCl2, 2 mM, 2 mM NaN3, 1 mM TCEP and 0.2 mM ADP.
cryo-EM vitrification conditions
Cryogen ETHANE；Manual backside blotting using Whatman filter paper No.5.
|
Resolution 4.10 Å |
| 5OOC Cryo-EM structure of jasplakinolide-stabilized F-actin in complex with ADP Deposited 2017-08-07 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 9ZK (4~{R},7~{R},10~{S},13~{S},15~{E},19~{S})-10-(4-azanylbutyl)-4-(4-hydroxyphenyl)-7-(1~{H}-indol-3-ylmethyl)-8,13,15,19-tetramethyl-1-oxa-5,8,11-triazacyclononadec-15-ene-2,6,9,12-tetrone × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；5 mM Tris pH 7.5, 2 mM NaN3, 1 mM DTT, 100 mM KCl and 2 mM MgCl2, 0.12 %(v/v) DMSO
cryo-EM vitrification conditions
Cryogen ETHANE；Manual backside blotting using Whatman filter paper No.5.
|
Resolution 3.60 Å |
| 5OOD Cryo-EM structure of jasplakinolide-stabilized F-actin in complex with ADP-Pi Deposited 2017-08-07 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 PO4 PHOSPHATE ION × 5 9ZK (4~{R},7~{R},10~{S},13~{S},15~{E},19~{S})-10-(4-azanylbutyl)-4-(4-hydroxyphenyl)-7-(1~{H}-indol-3-ylmethyl)-8,13,15,19-tetramethyl-1-oxa-5,8,11-triazacyclononadec-15-ene-2,6,9,12-tetrone × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；5 mM Tris pH 7.5, 2 mM NaN3, 1 mM DTT, 100 mM KCl and 2 mM MgCl2, 9.0 %(v/v) DMSO
cryo-EM vitrification conditions
Cryogen ETHANE；Manual backside blotting using Whatman filter paper No.5.
|
Resolution 3.70 Å |
| 5OOE Cryo-EM structure of F-actin in complex with AppNHp (AMPPNP) Deposited 2017-08-07 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；5 mM HEPES pH 7.5, 0.1 M KCl, 2 mM MgCl2, 2 mM, 2 mM NaN3, 0.5 mM TCEP and 0.4 mM AppNHp.
cryo-EM vitrification conditions
Cryogen ETHANE；Manual backside blotting using Whatman filter paper No.5.
|
Resolution 3.60 Å |
| 5OOF Cryo-EM structure of F-actin in complex with ADP-BeFx Deposited 2017-08-07 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；5 mM HEPES pH 7.5, 0.1 M KCl, 2 mM MgCl2, 2 mM, 2 mM NaN3, 1 mM TCEP, 0.2 mM ADP, 0.2 mM BeF2 and 5 mM NaF.
cryo-EM vitrification conditions
Cryogen ETHANE；Manual backside blotting using Whatman filter paper No.5.
|
Resolution 3.40 Å |
| 5UBO Mical-oxidized Actin complex with Gelsolin Segment 1 Deposited 2016-12-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–377（377 aa）
Fragment：unp residues 1-377
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.6；298 K；PEG 6000, NaCl, imidazole, ATP, calcium
|
Resolution 2.39 Å R-free 0.191 |
| 5YEE Crystal structure of LokiProfilin1/Rabbit Actin Complex Deposited 2017-09-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
1–377（377 aa）
|
Not recorded | LAB LATRUNCULIN B × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；100 mM Hepes, pH 7.0, 20 % w/v Polyethylene glycol 6,000, 200 mM NaCl, 10 mM ATP disodium salt
|
Resolution 1.81 Å R-free 0.249 |
| 5YPU Crystal structure of an actin monomer in complex with the nucleator Cordon-Bleu MET72NLE WH2-motif peptide Deposited 2017-11-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
7–374（368 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.9；298.15 K；20mM MES pH 4.9, 0.2mM CaCl2,2H2O, 20%(w/v) PEG 3,350
|
Resolution 2.00 Å R-free 0.240 |
| 5YPU Crystal structure of an actin monomer in complex with the nucleator Cordon-Bleu MET72NLE WH2-motif peptide Deposited 2017-11-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
7–374（368 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.9；298.15 K；20mM MES pH 4.9, 0.2mM CaCl2,2H2O, 20%(w/v) PEG 3,350
|
Resolution 2.00 Å R-free 0.240 |
| 5ZZA OdinProfilin/Rabbit Actin Complex Deposited 2018-05-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
5–377（373 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | LAB LATRUNCULIN B × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5；291 K；100 mM Citrate, 20% PEG6000
|
Resolution 1.53 Å R-free 0.179 |
| 5ZZB LokiProfilin2/Rabbit Actin Complex Deposited 2018-05-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
7–377（371 aa）
|
Not recorded | LAB LATRUNCULIN B × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；100 mM PCTP
25% PEG1500
|
Resolution 2.30 Å R-free 0.274 |
| 5ZZB LokiProfilin2/Rabbit Actin Complex Deposited 2018-05-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain D
7–377（371 aa）
|
Not recorded | LAB LATRUNCULIN B × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；100 mM PCTP
25% PEG1500
|
Resolution 2.30 Å R-free 0.274 |
| 6AV9 CryoEM structure of Mical Oxidized Actin (Class 1) Deposited 2017-09-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blot Force 1, Blot time 4s
|
Resolution 3.90 Å |
| 6AVB CryoEM structure of Mical Oxidized Actin (Class 1) Deposited 2017-09-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blot Force 1, Blot time 4s
|
Resolution 3.90 Å |
| 6BIH The Structure of the Actin-Smooth Muscle Myosin Motor Domain Complex in the Rigor State Deposited 2017-11-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric |
Chain C
1–377（377 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 7 MG MAGNESIUM ION × 7 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；actin buffer: 10 mM imidazole, 10 mM KCl, 1.0 mM MgCl2, 1.0 mM EGTA, 0.5 mM DTT, pH 7.4, myosin buffer: 10 mM imidazole, 10 mM KCl, 1.0 mM MgCl2, 1.0 mM EGTA, 0.5 mM DTT, pH 7.0
cryo-EM vitrification conditions
Cryogen ETHANE；Some specimens were frozen manually using a homemade plunger.
|
Resolution 6.00 Å |
| 6BIH The Structure of the Actin-Smooth Muscle Myosin Motor Domain Complex in the Rigor State Deposited 2017-11-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
1–377（377 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；actin buffer: 10 mM imidazole, 10 mM KCl, 1.0 mM MgCl2, 1.0 mM EGTA, 0.5 mM DTT, pH 7.4, myosin buffer: 10 mM imidazole, 10 mM KCl, 1.0 mM MgCl2, 1.0 mM EGTA, 0.5 mM DTT, pH 7.0
cryo-EM vitrification conditions
Cryogen ETHANE；Some specimens were frozen manually using a homemade plunger.
|
Resolution 6.00 Å |
| 6BNO Structure of bare actin filament Deposited 2017-11-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric |
Chain A
1–373（373 aa）
Chain B
1–373（373 aa）
Chain C
1–373（373 aa）
Chain D
1–373（373 aa）
Chain E
1–373（373 aa）
Chain F
1–373（373 aa）
Chain G
1–373（373 aa）
Chain H
1–373（373 aa）
|
Not recorded | MG MAGNESIUM ION × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；Buffer was filtered through 0.44 um filter and degassed.
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was applied to a glow-discharged holey carbon grid, incubated for 60 seconds and blotted for 3 seconds from the backside with filter paper.
|
Resolution 5.50 Å |
| 6BNP CryoEM structure of MyosinVI-actin complex in the rigor (nucleotide-free) state Deposited 2017-11-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric |
Chain A
1–373（373 aa）
Chain B
1–373（373 aa）
Chain C
1–373（373 aa）
Chain D
1–373（373 aa）
Chain E
1–373（373 aa）
Chain F
1–373（373 aa）
Chain G
1–373（373 aa）
Chain H
1–373（373 aa）
|
Not recorded | MG MAGNESIUM ION × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；Buffer was filtered through 0.44 um filter and degassed.
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was applied to a glow-discharged holey carbon grid. 3 uL actin was incubated for 60 seconds. 3 uL of myosin VI was added and incubated for 60 seconds. 3 uL solution was removed. An additional 3 uL of myosin VI was applied. After 60 seconds, 3 uL solution was removed, and the grid was blotted for 3 seconds from the backside with filter paper.
|
Resolution 4.60 Å |
| 6BNQ CryoEM structure of Myosin VI-Actin complex in the ADP state Deposited 2017-11-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric |
Chain A
1–373（373 aa）
Chain B
1–373（373 aa）
Chain C
1–373（373 aa）
Chain D
1–373（373 aa）
Chain E
1–373（373 aa）
Chain F
1–373（373 aa）
Chain G
1–373（373 aa）
Chain H
1–373（373 aa）
|
Not recorded | MG MAGNESIUM ION × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；Buffer was filtered through 0.44 um filter and degassed.
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was applied to a glow-discharged holey carbon grid. 3 uL actin was incubated for 60 seconds. 3 uL of myosin VI was added and incubated for 60 seconds. 3 uL solution was removed. An additional 3 uL of myosin VI was applied. After 60 seconds, 3 uL solution was removed, and the grid was blotted for 3 seconds from the backside with filter paper.
|
Resolution 5.50 Å |
| 6BNU Structure of bare actin filament, backbone-averaged with sidechains truncated to alanine Deposited 2017-11-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric |
Chain A
1–373（373 aa）
Chain B
1–373（373 aa）
Chain C
1–373（373 aa）
Chain D
1–373（373 aa）
Chain E
1–373（373 aa）
Chain F
1–373（373 aa）
Chain G
1–373（373 aa）
Chain H
1–373（373 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；Buffer was filtered through 0.44 um filter and degassed.
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was applied to a glow-discharged holey carbon grid, incubated for 60 seconds, and blotted for 3 seconds from the backside with filter paper.
|
Resolution 7.50 Å |
| 6BNV CryoEM structure of MyosinVI-actin complex in the rigor (nucleotide-free) state, backbone-averaged with side chains truncated to alanine Deposited 2017-11-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric |
Chain A
1–373（373 aa）
Chain B
1–373（373 aa）
Chain C
1–373（373 aa）
Chain D
1–373（373 aa）
Chain E
1–373（373 aa）
Chain F
1–373（373 aa）
Chain G
1–373（373 aa）
Chain H
1–373（373 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；Buffer was filtered through 0.44 um filter and degassed.
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was applied to a glow-discharged holey carbon grid. 3 uL actin was incubated for 60 seconds. 3 uL of myosin VI was added and incubated for 60 seconds. 3 uL solution was removed. An additional 3 uL of myosin VI was applied. After 60 seconds, 3 uL solution was removed, and the grid was blotted for 3 seconds from the backside with filter paper.
|
Resolution 4.60 Å |
| 6BNW CryoEM structure of Myosin VI-Actin complex in the ADP state, backbone-averaged with side chains truncated to alanine Deposited 2017-11-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric |
Chain A
1–373（373 aa）
Chain B
1–373（373 aa）
Chain C
1–373（373 aa）
Chain D
1–373（373 aa）
Chain E
1–373（373 aa）
Chain F
1–373（373 aa）
Chain G
1–373（373 aa）
Chain H
1–373（373 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；Buffer was filtered through 0.44 um filter and degassed
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was applied to a glow-discharged holey carbon grid. 3 uL actin was incubated for 60 seconds. 3 uL of myosin VI was added and incubated for 60 seconds. 3 uL solution was removed. An additional 3 uL of myosin VI was applied. After 60 seconds, 3 uL solution was removed, and the grid was blotted for 3 seconds from the backside with filter paper.
|
Resolution 5.50 Å |
| 6C1D High-Resolution Cryo-EM Structures of Actin-bound Myosin States Reveal the Mechanism of Myosin Force Sensing Deposited 2018-01-04 | Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: heptameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 6 MG MAGNESIUM ION × 6 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 6C1G High-Resolution Cryo-EM Structures of Actin-bound Myosin States Reveal the Mechanism of Myosin Force Sensing Deposited 2018-01-04 | Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Not recorded | MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 6 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 6C1H High-Resolution Cryo-EM Structures of Actin-bound Myosin States Reveal the Mechanism of Myosin Force Sensing Deposited 2018-01-04 | Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 6FHL Cryo-EM structure of F-actin in complex with ADP-Pi Deposited 2018-01-15 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 PO4 PHOSPHATE ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；5 mM HEPES pH 7.5, 0.05 M KCl, 2 mM MgCl2, 2 mM NaN3, 0.5 mM TCEP, 0.2 mM ADP, 50 mM potassium phosphate.
cryo-EM vitrification conditions
Cryogen ETHANE；8s blotting, 1s drain time, -25 force
|
Resolution 3.30 Å |
| 6FM2 CARP domain of mouse cyclase-associated protein 1 (CAP1) bound to ADP-actin Deposited 2018-01-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；0.1 M Tris-HCl, 0.2 M LiCl, 20% (w/v) PEG8000
|
Resolution 2.80 Å R-free 0.234 |
| 6GVC Structure of ArhGAP12 bound to G-Actin Deposited 2018-06-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
1–377（377 aa）
|
Not recorded | EDO 1,2-ETHANEDIOL × 2 LAB LATRUNCULIN B × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；20% PEG 3350,
0.2M Sodium Thiocyanate
0.1 M Bis Tris Propane pH6.5
|
Resolution 2.60 Å R-free 0.251 |
| 6GVC Structure of ArhGAP12 bound to G-Actin Deposited 2018-06-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–377（377 aa）
|
Not recorded | EDO 1,2-ETHANEDIOL × 3 LAB LATRUNCULIN B × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；20% PEG 3350,
0.2M Sodium Thiocyanate
0.1 M Bis Tris Propane pH6.5
|
Resolution 2.60 Å R-free 0.251 |
| 6GVC Structure of ArhGAP12 bound to G-Actin Deposited 2018-06-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
1–377（377 aa）
|
Not recorded | EDO 1,2-ETHANEDIOL × 1 LAB LATRUNCULIN B × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；20% PEG 3350,
0.2M Sodium Thiocyanate
0.1 M Bis Tris Propane pH6.5
|
Resolution 2.60 Å R-free 0.251 |
| 6GVC Structure of ArhGAP12 bound to G-Actin Deposited 2018-06-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain D
1–377（377 aa）
|
Not recorded | EDO 1,2-ETHANEDIOL × 2 LAB LATRUNCULIN B × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；20% PEG 3350,
0.2M Sodium Thiocyanate
0.1 M Bis Tris Propane pH6.5
|
Resolution 2.60 Å R-free 0.251 |
| 6JBK Crystal structure of an actin monomer in complex with the nucleator Cordon-Bleu WH2-motif peptide mutant. T22V Deposited 2019-01-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；298.15 K；20mM MES, pH 4.5, 0.2mM CaCl2.2H2O, 11% PEG 3350
|
Resolution 2.45 Å R-free 0.239 |
| 6JBK Crystal structure of an actin monomer in complex with the nucleator Cordon-Bleu WH2-motif peptide mutant. T22V Deposited 2019-01-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；298.15 K；20mM MES, pH 4.5, 0.2mM CaCl2.2H2O, 11% PEG 3350
|
Resolution 2.45 Å R-free 0.239 |
| 6JBK Crystal structure of an actin monomer in complex with the nucleator Cordon-Bleu WH2-motif peptide mutant. T22V Deposited 2019-01-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain E
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；298.15 K；20mM MES, pH 4.5, 0.2mM CaCl2.2H2O, 11% PEG 3350
|
Resolution 2.45 Å R-free 0.239 |
| 6JBK Crystal structure of an actin monomer in complex with the nucleator Cordon-Bleu WH2-motif peptide mutant. T22V Deposited 2019-01-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain G
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；298.15 K；20mM MES, pH 4.5, 0.2mM CaCl2.2H2O, 11% PEG 3350
|
Resolution 2.45 Å R-free 0.239 |
| 6JCU Crystal structure of an actin monomer in complex with a nucleator Cordon-Bleu WH2-motif peptide mutant. T22V, H11R Deposited 2019-01-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298.15 K；0.2M Ammonium nitrate, 20% PEG 3350
|
Resolution 2.30 Å R-free 0.230 |
| 6JCU Crystal structure of an actin monomer in complex with a nucleator Cordon-Bleu WH2-motif peptide mutant. T22V, H11R Deposited 2019-01-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298.15 K；0.2M Ammonium nitrate, 20% PEG 3350
|
Resolution 2.30 Å R-free 0.230 |
| 6JH8 Crystal structure of an actin monomer in complex with a chimeric peptide of Cordon-Bleu WH2 mutant and MIM. Deposited 2019-02-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；298.15 K；0.1M sodium acetate trihydrate, pH 4.6, 10% PEG 4000
|
Resolution 2.15 Å R-free 0.229 |
| 6JH9 Crystal structure of an actin monomer in complex with a chimeric peptide of Cordon-Bleu WH2 mutant and MIM. Lys18Arg Deposited 2019-02-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 2 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.8；298.15 K；0.1M sodium acetate trihydrate, pH 4.8, 8% PEG 4000
|
Resolution 1.74 Å R-free 0.206 |
| 6KN7 Structure of human cardiac thin filament in the calcium free state Deposited 2019-08-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 29 PDB declaration: 29-meric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
Chain F
3–377（375 aa）
Chain G
3–377（375 aa）
Chain H
3–377（375 aa）
Chain I
3–377（375 aa）
Chain J
3–377（375 aa）
Chain K
3–377（375 aa）
Chain L
3–377（375 aa）
Chain M
3–377（375 aa）
Chain N
3–377（375 aa）
Chain O
3–377（375 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 15 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.60 Å |
| 6KN8 Structure of human cardiac thin filament in the calcium bound state Deposited 2019-08-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 29 PDB declaration: 29-meric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
Chain F
3–377（375 aa）
Chain G
3–377（375 aa）
Chain H
3–377（375 aa）
Chain I
3–377（375 aa）
Chain J
3–377（375 aa）
Chain K
3–377（375 aa）
Chain L
3–377（375 aa）
Chain M
3–377（375 aa）
Chain N
3–377（375 aa）
Chain O
3–377（375 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 15 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.80 Å |
| 6MGO Structure of rabbit actin in complex with Mycalolide B Deposited 2018-09-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–377（377 aa）
|
Not recorded | JQV Mycalolide B × 1 CA CALCIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；PEG 8000, magnesium acetate, cacodylate
|
Resolution 2.20 Å R-free 0.195 |
| 6NAS Ternary Complex of Ac-Alpha-Actin with Profilin and AcCoA-NAA80 Deposited 2018-12-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 LAB LATRUNCULIN B × 1 GOL GLYCEROL × 3 ACO ACETYL COENZYME *A × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；290 K；16% Peg3350, 0.1 MES pH 6.5, 0.2M NH4NO3
|
Resolution 2.90 Å R-free 0.239 |
| 6NBE Ternary Complex of Ac-Alpha-Actin with Profilin and CoA-NAA80 Deposited 2018-12-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 LAB LATRUNCULIN B × 1 GOL GLYCEROL × 3 COA COENZYME A × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；290 K；16% Peg3350, 0.1 MES pH 6.5, 0.2M NH4NO3
|
Resolution 2.00 Å R-free 0.184 |
| 6QRI Structure of rabbit G-actin in complex with chivosazole A Deposited 2019-02-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 CV9 (2~{R},3~{R},5~{S},6~{E},8~{E},10~{Z},12~{S},13~{R},16~{Z},18~{E},20~{Z},22~{E},24~{R},25~{S},26~{E},28~{Z})-13-[(2~{S},3~{S},5~{S})-3,5-bis(oxidanyl)hexan-2-yl]-25-[(2~{R},3~{R},4~{S},5~{R},6~{R})-3,4-dimethoxy-6-methyl-5-oxidanyl-oxan-2-yl]oxy-3-methoxy-2,12,22,24-tetramethyl-5-oxidanyl-14,32-dioxa-33-azabicyclo[28.2.1]tritriaconta-1(33),6,8,10,16,18,20,22,26,28,30-undecaen-15-one × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；0.5 M lithium chloride, 100 mM Tris(hydroxymethyl)aminomethane hydrochloride (Tris-HCl), 28 % (w/v) polyethylenglycol 6000, pH 8.5
|
Resolution 2.40 Å R-free 0.268 |
| 6QRI Structure of rabbit G-actin in complex with chivosazole A Deposited 2019-02-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 CV9 (2~{R},3~{R},5~{S},6~{E},8~{E},10~{Z},12~{S},13~{R},16~{Z},18~{E},20~{Z},22~{E},24~{R},25~{S},26~{E},28~{Z})-13-[(2~{S},3~{S},5~{S})-3,5-bis(oxidanyl)hexan-2-yl]-25-[(2~{R},3~{R},4~{S},5~{R},6~{R})-3,4-dimethoxy-6-methyl-5-oxidanyl-oxan-2-yl]oxy-3-methoxy-2,12,22,24-tetramethyl-5-oxidanyl-14,32-dioxa-33-azabicyclo[28.2.1]tritriaconta-1(33),6,8,10,16,18,20,22,26,28,30-undecaen-15-one × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；0.5 M lithium chloride, 100 mM Tris(hydroxymethyl)aminomethane hydrochloride (Tris-HCl), 28 % (w/v) polyethylenglycol 6000, pH 8.5
|
Resolution 2.40 Å R-free 0.268 |
| 6RSW HFD domain of mouse CAP1 bound to the pointed end of G-actin Deposited 2019-05-22 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；0.1M HEPES, 0.1 mM KCl, 10% PEG4000 (w/v)
|
Resolution 1.95 Å R-free 0.194 |
| 6T1Y Cryo-EM structure of phalloidin-stabilized F-actin (copolymerized) Deposited 2019-10-07 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；5 mM Tris pH 7.5, 2 mM NaN3, 1 mM DTT, 100 mM KCl and 2 mM MgCl2, 0.01 %(v/v) MeOH, 0.02 %(v/w) Tween 20
cryo-EM vitrification conditions
Cryogen ETHANE；Manual backside blotting using Whatman filter paper No.5.
|
Resolution 3.30 Å |
| 6T20 Cryo-EM structure of phalloidin-stabilized F-actin (aged) Deposited 2019-10-07 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；5 mM Tris pH 7.5, 2 mM NaN3, 1 mM DTT, 100 mM KCl and 2 mM MgCl2, 2.0 %(v/v) MeOH, 0.03 %(v/w) Tween 20
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 6T23 Cryo-EM structure of jasplakinolide-stabilized F-actin (aged) Deposited 2019-10-07 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 PO4 PHOSPHATE ION × 5 MG MAGNESIUM ION × 5 9ZK (4~{R},7~{R},10~{S},13~{S},15~{E},19~{S})-10-(4-azanylbutyl)-4-(4-hydroxyphenyl)-7-(1~{H}-indol-3-ylmethyl)-8,13,15,19-tetramethyl-1-oxa-5,8,11-triazacyclononadec-15-ene-2,6,9,12-tetrone × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；5 mM Tris pH 7.5, 2 mM NaN3, 1 mM DTT, 100 mM KCl and 2 mM MgCl2, 0.2 %(v/v) DMSO, 0.02 %(v/w) Tween 20
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 6T24 Cryo-EM structure of jasplakinolide-stabilized F-actin (aged) Deposited 2019-10-07 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 PO4 PHOSPHATE ION × 5 MG MAGNESIUM ION × 5 9ZK (4~{R},7~{R},10~{S},13~{S},15~{E},19~{S})-10-(4-azanylbutyl)-4-(4-hydroxyphenyl)-7-(1~{H}-indol-3-ylmethyl)-8,13,15,19-tetramethyl-1-oxa-5,8,11-triazacyclononadec-15-ene-2,6,9,12-tetrone × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；5 mM Tris pH 7.5, 2 mM NaN3, 1 mM DTT, 100 mM KCl and 2 mM MgCl2, 0.5 %(v/v) DMSO, 0.02 %(v/w) Tween 20
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 6T25 Cryo-EM structure of phalloidin-Alexa Flour-546-stabilized F-actin (copolymerized) Deposited 2019-10-07 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；5 mM Tris pH 7.5, 2 mM NaN3, 1 mM DTT, 100 mM KCl and 2 mM MgCl2, 1.5 %(v/v) MeOH, 0.02 %(v/w) Tween 20
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 6U96 Actin phalloidin at BeFx state Deposited 2019-09-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；pH7.4, 10mM Tris, 50mM KCl, 1 mM MgC2, 0.2mM CaCl2, 1mM ATP, 1mM DTT, 0.2mM EGTA, 0.2mM BeCl2, 5mM NaF
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 6UBY Isolated cofilin bound to an actin filament Deposited 2019-09-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
Chain H
1–377（377 aa）
|
Not recorded | MG MAGNESIUM ION × 7 ADP ADENOSINE-5'-DIPHOSPHATE × 7 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.50 Å |
| 6UC0 Isolated S3D-cofilin bound to an actin filament Deposited 2019-09-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
|
Not recorded | MG MAGNESIUM ION × 7 ADP ADENOSINE-5'-DIPHOSPHATE × 7 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.50 Å |
| 6UC4 Barbed end side of a cofilactin cluster Deposited 2019-09-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
Chain H
1–377（377 aa）
Chain J
1–377（377 aa）
Chain K
1–377（377 aa）
Chain L
1–377（377 aa）
|
Not recorded | MG MAGNESIUM ION × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 9 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 9.20 Å |
| 6VAO Human cofilin-1 decorated actin filament Deposited 2019-12-17 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
|
Not recorded | MG MAGNESIUM ION × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 6VAU Bare actin filament from a partially cofilin-decorated sample Deposited 2019-12-17 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
|
Not recorded | MG MAGNESIUM ION × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 6VEC Cryo-EM structure of F-actin/Plastin2-ABD2 complex Deposited 2019-12-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
Chain H
1–377（377 aa）
Chain I
1–377（377 aa）
Chain J
1–377（377 aa）
Chain K
1–377（377 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 11 MG MAGNESIUM ION × 11 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 6W17 Structure of Dip1-activated Arp2/3 complex with nucleated actin filament Deposited 2020-03-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 17 PDB declaration: heptadecameric |
Chain I
1–377（377 aa）
Chain J
1–377（377 aa）
Chain K
1–377（377 aa）
Chain L
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 6W7V Structure of rabbit actin in complex with truncated analog of Mycalolide B Deposited 2020-03-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–377（377 aa）
|
Not recorded | CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 LAB LATRUNCULIN B × 1 EDO 1,2-ETHANEDIOL × 3 TFJ (1E,3R,4R,5S,6R,9S,10S,12S)-12-[(4-aminobutanoyl)oxy]-1-[ethyl(formyl)amino]-4,10-dimethoxy-3,5,9,13-tetramethyltetradec-1-en-6-yl (2R)-oxolane-2-carboxylate × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；0.1M Tris-HCl, pH 8.5, 25% PEG3350
|
Resolution 1.70 Å R-free 0.213 |
| 6WVT Structural basis of alphaE-catenin - F-actin catch bond behavior Deposited 2020-05-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain B
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
Chain H
1–377（377 aa）
Chain I
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 6 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.56 Å |
| 6YP9 Rabbit muscle actin in complex with ADF-H and ATP-ATTO-488 Deposited 2020-04-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；0.1 M sodium cacodylate (pH 6.0) and 15% (w/v) PEG 4000
|
Resolution 2.56 Å R-free 0.230 |
| 7AD9 Structure of the Lifeact-F-actin complex Deposited 2020-09-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric |
Chain B
1–377（377 aa）
Chain D
1–377（377 aa）
Chain F
1–377（377 aa）
Chain H
1–377（377 aa）
Chain I
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 PO4 PHOSPHATE ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8；120 mM KCl, 20 mM Tris pH 8, 2 mM MgCl2, 1 mM DTT, and 0.02% w/v Tween-20
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 7AHN Cryo-EM structure of F-actin stabilized by cis-optoJASP-8 Deposited 2020-09-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 PO4 PHOSPHATE ION × 5 RLZ ~{N}-[4-[(4~{R},7~{R},10~{S},13~{S},15~{E},19~{S})-4-(4-hydroxyphenyl)-7-(1~{H}-indol-3-ylmethyl)-8,13,15,19-tetramethyl-2,6,9,12-tetrakis(oxidanylidene)-1-oxa-5,8,11-triazacyclononadec-15-en-10-yl]butyl]-~{N}'-[5-methoxy-2-[(~{Z})-(3,4,5-trimethoxyphenyl)diazenyl]phenyl]butanediamide × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；5 mM Tris pH 7.5, 2 mM NaN3, 1 mM DTT, 100 mM KCl and 2 mM MgCl2, 0.4 %(v/v) DMSO, 0.02 %(v/w) Tween 20
cryo-EM vitrification conditions
Cryogen ETHANE；1.5 mul sample, automatic blotting for 7-7.5s, blot force -25, drain time 1s.
|
Resolution 2.90 Å |
| 7AHQ Cryo-EM structure of F-actin stabilized by trans-optoJASP-8 Deposited 2020-09-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 PO4 PHOSPHATE ION × 5 MG MAGNESIUM ION × 5 RLZ ~{N}-[4-[(4~{R},7~{R},10~{S},13~{S},15~{E},19~{S})-4-(4-hydroxyphenyl)-7-(1~{H}-indol-3-ylmethyl)-8,13,15,19-tetramethyl-2,6,9,12-tetrakis(oxidanylidene)-1-oxa-5,8,11-triazacyclononadec-15-en-10-yl]butyl]-~{N}'-[5-methoxy-2-[(~{Z})-(3,4,5-trimethoxyphenyl)diazenyl]phenyl]butanediamide × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；5 mM Tris pH 7.5, 2 mM NaN3, 1 mM DTT, 100 mM KCl and 2 mM MgCl2, 0.7 %(v/v) DMSO, 0.02 %(v/w) Tween 20
cryo-EM vitrification conditions
Cryogen ETHANE；1.5 mul sample, automatic blotting for 7-7.5s, blot force -25, drain time 1s.
|
Resolution 3.60 Å |
| 7C2F Crystal Structure of the Thorarchaeota ProGel/rabbit actin complex Deposited 2020-05-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | LAB LATRUNCULIN B × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；291 K；0.1 M MES pH 6.0
0.2 M magnesium chloride hexahydrate
20% w/v polyethylene glycol 6000
|
Resolution 2.03 Å R-free 0.231 |
| 7C2F Crystal Structure of the Thorarchaeota ProGel/rabbit actin complex Deposited 2020-05-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | LAB LATRUNCULIN B × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；291 K；0.1 M MES pH 6.0
0.2 M magnesium chloride hexahydrate
20% w/v polyethylene glycol 6000
|
Resolution 2.03 Å R-free 0.231 |
| 7C2G Crystal Structure of the Thorarchaeota 2DGel/rabbit actin complex Deposited 2020-05-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；291 K；0.1 M Bis-Tris pH 5.5
0.2 M magnesium chloride hexahydrate
25% w/v polyethylene glycol 3350
|
Resolution 1.71 Å R-free 0.203 |
| 7C2H Crystal Structure of the Thorarchaeota 2DGel3/rabbit actin complex Deposited 2020-05-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；291 K；0.1 M CHES pH 9.5
20% w/v polyethylene glycol 8000
|
Resolution 2.35 Å R-free 0.238 |
| 7CCC The structure of the actin filament uncapping complex mediated by twinfilin Deposited 2020-06-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric |
Chain A
1–377（377 aa）
Chain E
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；12% PEG 8000, 10% glycerol, 500 mM potassium chloride
|
Resolution 3.20 Å R-free 0.238 |
| 7NXV Crystal structure of the complex of DNase I/G-actin/PPP1R15A_582-621 Deposited 2021-03-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
3–377（375 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 5；293 K；10% PEG4000, 0.1M Acetate
|
Resolution 2.55 Å R-free 0.248 |
| 7NXV Crystal structure of the complex of DNase I/G-actin/PPP1R15A_582-621 Deposited 2021-03-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Other combination Heteromer；Protein × 3 PDB declaration: trimeric |
Chain D
3–377（375 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 5；293 K；10% PEG4000, 0.1M Acetate
|
Resolution 2.55 Å R-free 0.248 |
| 7NZM Cryo-EM structure of pre-dephosphorylation complex of phosphorylated eIF2alpha with trapped holophosphatase (PP1A_D64A/PPP1R15A/G-actin/DNase I) Deposited 2021-03-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 5 PDB declaration: pentameric |
Chain A
3–377（375 aa）
|
Not recorded | MN MANGANESE (II) ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；0.22mM Triton X-100 was added into the solution before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.96 Å |
| 7P1G Structure of the P. aeruginosa ExoY-F-actin complex Deposited 2021-07-01 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 15 PDB declaration: pentadecameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
|
Not recorded | MG MAGNESIUM ION × 10 GH3 3'-DEOXY-GUANOSINE-5'-TRIPHOSPHATE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 5 PO4 PHOSPHATE ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 7PLT Cryo-EM structure of the actomyosin-V complex in the rigor state (central 1er) Deposited 2021-09-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.30 Å |
| 7PLU Cryo-EM structure of the actomyosin-V complex in the rigor state (central 3er/2er) Deposited 2021-09-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain C
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 3 MG MAGNESIUM ION × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.20 Å |
| 7PLV Cryo-EM structure of the actomyosin-V complex in the rigor state (central 1er, class 1) Deposited 2021-09-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.50 Å |
| 7PLW Cryo-EM structure of the actomyosin-V complex in the rigor state (central 1er, class 2) Deposited 2021-09-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.50 Å |
| 7PLX Cryo-EM structure of the actomyosin-V complex in the rigor state (central 1er, class 4) Deposited 2021-09-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.60 Å |
| 7PLY Cryo-EM structure of the actomyosin-V complex in the rigor state (central 1er, young JASP-stabilized F-actin) Deposited 2021-09-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1 MG MAGNESIUM ION × 1 9UE Jasplakinolide × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.20 Å |
| 7PLZ Cryo-EM structure of the actomyosin-V complex in the rigor state (central 3er/2er, young JASP-stabilized F-actin) Deposited 2021-09-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric |
Chain C
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 3 PO4 PHOSPHATE ION × 3 MG MAGNESIUM ION × 3 9UE Jasplakinolide × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.20 Å |
| 7PM0 Cryo-EM structure of the actomyosin-V complex in the rigor state (central 1er, young JASP-stabilized F-actin, class 1) Deposited 2021-09-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1 MG MAGNESIUM ION × 1 9UE Jasplakinolide × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.60 Å |
| 7PM1 Cryo-EM structure of the actomyosin-V complex in the rigor state (central 1er, young JASP-stabilized F-actin, class 2) Deposited 2021-09-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1 MG MAGNESIUM ION × 1 9UE Jasplakinolide × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.50 Å |
| 7PM2 Cryo-EM structure of the actomyosin-V complex in the rigor state (central 1er, young JASP-stabilized F-actin, class 4) Deposited 2021-09-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1 MG MAGNESIUM ION × 1 9UE Jasplakinolide × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.60 Å |
| 7PM3 Cryo-EM structure of young JASP-stabilized F-actin (central 3er) Deposited 2021-09-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 3 PO4 PHOSPHATE ION × 3 MG MAGNESIUM ION × 3 9UE Jasplakinolide × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 7PM5 Cryo-EM structure of the actomyosin-V complex in the strong-ADP state (central 1er) Deposited 2021-09-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.10 Å |
| 7PM6 Cryo-EM structure of the actomyosin-V complex in the strong-ADP state (central 3er/2er) Deposited 2021-09-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain C
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.00 Å |
| 7PM7 Cryo-EM structure of the actomyosin-V complex in the strong-ADP state (central 1er, class 2) Deposited 2021-09-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.50 Å |
| 7PM8 Cryo-EM structure of the actomyosin-V complex in the strong-ADP state (central 1er, class 3) Deposited 2021-09-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.50 Å |
| 7PM9 Cryo-EM structure of the actomyosin-V complex in the strong-ADP state (central 1er, class 4) Deposited 2021-09-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.70 Å |
| 7PMA Cryo-EM structure of the actomyosin-V complex in the strong-ADP state (central 1er, class 5) Deposited 2021-09-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.60 Å |
| 7PMB Cryo-EM structure of the actomyosin-V complex in the strong-ADP state (central 1er, class 6) Deposited 2021-09-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.60 Å |
| 7PMC Cryo-EM structure of the actomyosin-V complex in the strong-ADP state (central 1er, class 7) Deposited 2021-09-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.70 Å |
| 7PMD Cryo-EM structure of the actomyosin-V complex in the post-rigor transition state (AppNHp, central 1er) Deposited 2021-09-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 MG MAGNESIUM ION × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration, two data sets combined
|
Resolution 2.90 Å |
| 7PME Cryo-EM structure of the actomyosin-V complex in the post-rigor transition state (AppNHp, central 3er/2er) Deposited 2021-09-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain C
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 MG MAGNESIUM ION × 7 ADP ADENOSINE-5'-DIPHOSPHATE × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration, two data sets combined
|
Resolution 2.90 Å |
| 7PMF Cryo-EM structure of the actomyosin-V complex in the post-rigor transition state (AppNHp, central 1er, class 1) Deposited 2021-09-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 MG MAGNESIUM ION × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration, two data sets combined
|
Resolution 3.40 Å |
| 7PMG Cryo-EM structure of the actomyosin-V complex in the post-rigor transition state (AppNHp, central 1er, class 3) Deposited 2021-09-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 MG MAGNESIUM ION × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration, two data sets combined
|
Resolution 3.30 Å |
| 7PMH Cryo-EM structure of the actomyosin-V complex in the post-rigor transition state (AppNHp, central 1er, class 4) Deposited 2021-09-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 MG MAGNESIUM ION × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration, two data sets combined
|
Resolution 3.40 Å |
| 7PMI Cryo-EM structure of the actomyosin-V complex in the post-rigor transition state (AppNHp, central 1er, class 5) Deposited 2021-09-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 MG MAGNESIUM ION × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration, two data sets combined
|
Resolution 3.30 Å |
| 7PMJ Cryo-EM structure of the actomyosin-V complex in the post-rigor transition state (AppNHp, central 1er, class 6) Deposited 2021-09-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 MG MAGNESIUM ION × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration, two data sets combined
|
Resolution 3.40 Å |
| 7PML Cryo-EM structure of the actomyosin-V complex in the post-rigor transition state (AppNHp, central 1er, class 8) Deposited 2021-09-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 MG MAGNESIUM ION × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration, two data sets combined
|
Resolution 3.30 Å |
| 7T5Q Cryo-EM Structure of a Transition State of Arp2/3 Complex Activation Deposited 2021-12-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 10 PDB declaration: decameric |
Chain H
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE；Grids were manually blotted for 3 seconds with Whatman 41 filter paper and manually plunged using a Leica EM CPC manual plunger.
|
Resolution 3.40 Å |
| 7TPT Single-particle Cryo-EM structure of Arp2/3 complex at branched-actin junction. Deposited 2022-01-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 36 PDB declaration: 36-meric |
Chain H
1–377（377 aa）
Chain I
1–377（377 aa）
Chain J
1–377（377 aa）
Chain K
1–377（377 aa）
Chain L
1–377（377 aa）
Chain M
1–377（377 aa）
Chain N
1–377（377 aa）
Chain O
1–377（377 aa）
Chain P
1–377（377 aa）
Chain Q
1–377（377 aa）
Chain R
1–377（377 aa）
Chain S
1–377（377 aa）
Chain T
1–377（377 aa）
Chain U
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 16 ADP ADENOSINE-5'-DIPHOSPHATE × 16 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 7U8K Magic Angle Spinning NMR Structure of Human Cofilin-2 Assembled on Actin Filaments Deposited 2022-03-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
Chain F
3–377（375 aa）
Chain G
3–377（375 aa）
Chain H
3–377（375 aa）
Chain I
3–377（375 aa）
Chain J
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
SOLID-STATE NMR
NMR measurement conditions
pH 6.6；273 K；Ionic strength (raw mmCIF value) 50；Pressure 1
NMR sample composition
20.8 % w/w [U-13C; U-15N] human cofilin-2, 79.2 % w/w F-actin, Solid | Solid
NMR sample composition
20.8 % w/w [1,6-13C]-glucose, U-15N human cofilin-2, 79.2 % w/w F-actin, Solid | Solid
NMR sample composition
20.8 % w/w [2-13C]-glucose, U-15N human cofilin-2, 79.2 % w/w F-actin, Solid | Solid
|
Resolution not provided |
| 7UTI ALTERNATIVE MODELING OF TROPOMYOSIN IN HUMAN CARDIAC THIN FILAMENT IN THE CALCIUM BOUND STATE Deposited 2022-04-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric |
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
Chain H
1–377（377 aa）
Chain I
1–377（377 aa）
Chain J
1–377（377 aa）
Chain K
1–377（377 aa）
Chain L
1–377（377 aa）
Chain M
1–377（377 aa）
Chain N
1–377（377 aa）
Chain O
1–377（377 aa）
Chain P
1–377（377 aa）
Chain Q
1–377（377 aa）
Chain R
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 16 MG MAGNESIUM ION × 16 CA CALCIUM ION × 6 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.80 Å |
| 7UTJ Cryogenic electron microscopy 3D map of F-actin bound by human dimeric alpha-catenin Deposited 2022-04-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 6 MG MAGNESIUM ION × 6 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.77 Å |
| 7UTL ALTERNATIVE MODELING OF TROPOMYOSIN IN HUMAN CARDIAC THIN FILAMENT IN THE CALCIUM FREE STATE Deposited 2022-04-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
Chain H
1–377（377 aa）
Chain I
1–377（377 aa）
Chain J
1–377（377 aa）
Chain K
1–377（377 aa）
Chain L
1–377（377 aa）
Chain M
1–377（377 aa）
Chain N
1–377（377 aa）
Chain O
1–377（377 aa）
Chain P
1–377（377 aa）
Chain Q
1–377（377 aa）
Chain R
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 18 MG MAGNESIUM ION × 18 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.60 Å |
| 7UUW Cryogenic electron microscopy 3D map of F-actin bound by the Actin Binding Domain of alpha-catenin ortholog, HMP1 Deposited 2022-04-29 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 6 MG MAGNESIUM ION × 6 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.36 Å |
| 7UXF Cryogenic electron microscopy 3D map of F-actin Deposited 2022-05-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 6 MG MAGNESIUM ION × 6 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 7WHF Heimdallarchaeota gelsolin (2DGel) bound to rabbit actin Deposited 2021-12-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 9 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；297 K；0.4 mM Heimdallarchaeota 2DGel
0.4 mM rabbit actin
1 mM CaCl2
0.1 M HEPES pH 7.0
10% w/v polyethylene glycol 6000
|
Resolution 2.10 Å R-free 0.216 |
| 7WHF Heimdallarchaeota gelsolin (2DGel) bound to rabbit actin Deposited 2021-12-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 9 GOL GLYCEROL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；297 K；0.4 mM Heimdallarchaeota 2DGel
0.4 mM rabbit actin
1 mM CaCl2
0.1 M HEPES pH 7.0
10% w/v polyethylene glycol 6000
|
Resolution 2.10 Å R-free 0.216 |
| 7WHG Lokiarchaeota gelsolin (2DGel) bound to two molecules of rabbit actin Deposited 2021-12-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 2 CA CALCIUM ION × 9 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；0.4 mM Loki2DGel
0.4 mM rabbit actin
0.1 M Tris-HCl, pH 7.0
0.2 M magnesium chloride hexahydrate
10% w/v polyethylene glycol 8000
1 mM CaCl2
|
Resolution 3.25 Å R-free 0.232 |
| 7Z7H Structure of P. luminescens TccC3-F-actin complex Deposited 2022-03-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 APR ADENOSINE-5-DIPHOSPHORIBOSE × 1 NCA NICOTINAMIDE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 7Z7I Structure of ADP-ribosylated F-actin Deposited 2022-03-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 APR ADENOSINE-5-DIPHOSPHORIBOSE × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 8A2R Cryo-EM structure of F-actin in the Mg2+-ADP-BeF3- nucleotide state. Deposited 2022-06-06 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 BEF BERYLLIUM TRIFLUORIDE ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；F-buffer:
5 mM Tris pH 7.5, 100 mM KCl, 2 mM MgCl2, 2 mM NaN3, 1 mM DTT, 0.75 mM BeF2, 5 mM NaF.
cryo-EM vitrification conditions
Cryogen ETHANE；The Vitrobot was operated at 13 degrees celsius and the samples were blotted for 9 seconds with a blot force of -25.
|
Resolution 2.17 Å |
| 8A2S Cryo-EM structure of F-actin in the Mg2+-ADP-Pi nucleotide state. Deposited 2022-06-06 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 PO4 PHOSPHATE ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；F-phosphate buffer:
5 mM Tris, 100 mM KCl, 2 mM MgCl2, 2 mM NaN3, 1 mM DTT, 50 mM potassium phosphate pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE；The Vitrobot was operated at 13 degrees celsius and the samples were blotted for 9 seconds with a blot force of -25.
|
Resolution 2.22 Å |
| 8A2T Cryo-EM structure of F-actin in the Mg2+-ADP nucleotide state. Deposited 2022-06-06 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；F-buffer:
5 mM Tris pH 7.5, 100 mM KCl, 2 mM MgCl2, 2 mM NaN3, 1 mM DTT
cryo-EM vitrification conditions
Cryogen ETHANE；The Vitrobot was operated at 13 degrees celsius and the samples were blotted for 9 seconds with a blot force of -25.
|
Resolution 2.24 Å |
| 8A2U Cryo-EM structure of F-actin in the Ca2+-ADP-BeF3- nucleotide state. Deposited 2022-06-06 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 CA CALCIUM ION × 5 BEF BERYLLIUM TRIFLUORIDE ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；F-buffer:
5 mM Tris pH 7.5, 100 mM KCl, 2 mM CaCl2, 2 mM NaN3, 1 mM DTT, 0.75 mM BeF2, 5 mM NaF.
cryo-EM vitrification conditions
Cryogen ETHANE；The Vitrobot was operated at 13 degrees celsius and the samples were blotted for 9 seconds with a blot force of -25.
|
Resolution 2.21 Å |
| 8A2Y Cryo-EM structure of F-actin in the Ca2+-ADP-Pi nucleotide state. Deposited 2022-06-06 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 CA CALCIUM ION × 5 PO4 PHOSPHATE ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；F-phosphate buffer:
5 mM Tris, 100 mM KCl, 2 mM CaCl2, 2 mM NaN3, 1 mM DTT, 50 mM potassium phosphate pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE；The Vitrobot was operated at 13 degrees celsius and the samples were blotted for 9 seconds with a blot force of -25.
|
Resolution 2.15 Å |
| 8A2Z Cryo-EM structure of F-actin in the Ca2+-ADP nucleotide state. Deposited 2022-06-06 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 CA CALCIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；F-buffer:
5 mM Tris pH 7.5, 100 mM KCl, 2 mM CaCl2, 2 mM NaN3, 1 mM DTT
cryo-EM vitrification conditions
Cryogen ETHANE；The Vitrobot was operated at 13 degrees celsius and the samples were blotted for 9 seconds with a blot force of -25.
|
Resolution 2.15 Å |
| 8BJH chimera of the inactive ExoY Nucleotidyl Cyclase domain from Vibrio nigripulchritudo MARTX toxin, with the double mutation K3528M and K3535I, fused to a proline-Rich-Domain (PRD) and profilin, bound to Latrunculin B-ADP-Mg-actin Deposited 2022-11-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 LAB LATRUNCULIN B × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 2 GOL GLYCEROL × 3 PEO HYDROGEN PEROXIDE × 8 SO4 SULFATE ION × 2 PEG DI(HYDROXYETHYL)ETHER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；30% PEG4000 0.2M LISO4 0.1M TRIS PH8.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 1.69 Å R-free 0.212 |
| 8BJI chimera of ExoY Nucleotidyl Cyclase domain from Vibrio nigripulchritudo fused to a proline-Rich-Domain (PRD) and profilin, bound to ADP-Mg-actin and a sulfate ion Deposited 2022-11-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 2 GOL GLYCEROL × 4 PEG DI(HYDROXYETHYL)ETHER × 2 PEO HYDROGEN PEROXIDE × 14 SO4 SULFATE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
EVAPORATION；pH 8.5；293 K；30% peg 4000, 0.2 M Lithium Sulfate (LiSO4), 0.1 M TrisHCl pH8.5
|
Resolution 1.75 Å R-free 0.212 |
| 8BJJ ExoY Nucleotidyl Cyclase domain from Vibrio nigripulchritudo MARTX toxin, bound to ATP-Mg-actin, human profilin 1 and a sulfate ion Deposited 2022-11-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
3–377（375 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 LAB LATRUNCULIN B × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 2 SO4 SULFATE ION × 4 PEO HYDROGEN PEROXIDE × 2 PG4 TETRAETHYLENE GLYCOL × 1 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；291 K；30% peg3000
0.3M LiSO4
0.1M Tris pH8.5
3% Dioxane
|
Resolution 1.70 Å R-free 0.195 |
| 8BO1 ExoY Nucleotidyl Cyclase domain from Vibrio nigripulchritudo MARTX toxin, bound to Latrunculin-B-ATP-Mg-actin, and 3'-DEOXYADENOSINE-5'-TRIPHOSPHATE and 2 Mg ions Deposited 2022-11-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
|
Not recorded | LAB LATRUNCULIN B × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 3 SO4 SULFATE ION × 1 3AT 3'-DEOXYADENOSINE-5'-TRIPHOSPHATE × 1 MN MANGANESE (II) ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；291 K；26 % peg 3350, 26 % Glycerol, 30 mM LiSO4, 0.1M TrisHCl pH8.5, 3 % Dioxane,
|
Resolution 2.50 Å R-free 0.231 |
| 8BO1 ExoY Nucleotidyl Cyclase domain from Vibrio nigripulchritudo MARTX toxin, bound to Latrunculin-B-ATP-Mg-actin, and 3'-DEOXYADENOSINE-5'-TRIPHOSPHATE and 2 Mg ions Deposited 2022-11-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
3–377（375 aa）
|
Not recorded | LAB LATRUNCULIN B × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 3 SO4 SULFATE ION × 2 3AT 3'-DEOXYADENOSINE-5'-TRIPHOSPHATE × 1 MN MANGANESE (II) ION × 6 AZI AZIDE ION × 6 CL CHLORIDE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；291 K；26 % peg 3350, 26 % Glycerol, 30 mM LiSO4, 0.1M TrisHCl pH8.5, 3 % Dioxane,
|
Resolution 2.50 Å R-free 0.231 |
| 8BR0 ExoY Nucleotidyl Cyclase domain from Vibrio nigripulchritudo MARTX toxin (residue Q3455 to L3863) in complex with 3'deoxyCTP and two manganese cations bound to Latrunculin-B-ADP-Mn-actin Deposited 2022-11-22 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
|
Not recorded | LAB LATRUNCULIN B × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 3 CH1 3'-DEOXY-CYTIDINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；10mg/mL binary complex in the presence of
15.2 mM 3primedCTP1.6 mM ADP, 20 mM MgCl2, 0.2 mM Latrunculin B, 23 mM KCl, 70 mM LiCl, 8 mM HEPES pH 8.5, 4 mM TCEP and mixed with 17 % PEG4000, 17 % Glycerol, 0.01 M Li2SO4, 0.1 M Tris pH 8.5, 5 mM MgCl2, 15 mM MnCl2, 1% 1-Butyl-2,3-dimethylimidazolium tetrafluoroborate (ionic liquid 18 from the Ionic Liquid Screen (Hampton Research)) in a 1:1.2 v/v hanging drop
|
Resolution 2.22 Å R-free 0.243 |
| 8BR0 ExoY Nucleotidyl Cyclase domain from Vibrio nigripulchritudo MARTX toxin (residue Q3455 to L3863) in complex with 3'deoxyCTP and two manganese cations bound to Latrunculin-B-ADP-Mn-actin Deposited 2022-11-22 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
3–377（375 aa）
|
Not recorded | LAB LATRUNCULIN B × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 3 CH1 3'-DEOXY-CYTIDINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；10mg/mL binary complex in the presence of
15.2 mM 3primedCTP1.6 mM ADP, 20 mM MgCl2, 0.2 mM Latrunculin B, 23 mM KCl, 70 mM LiCl, 8 mM HEPES pH 8.5, 4 mM TCEP and mixed with 17 % PEG4000, 17 % Glycerol, 0.01 M Li2SO4, 0.1 M Tris pH 8.5, 5 mM MgCl2, 15 mM MnCl2, 1% 1-Butyl-2,3-dimethylimidazolium tetrafluoroborate (ionic liquid 18 from the Ionic Liquid Screen (Hampton Research)) in a 1:1.2 v/v hanging drop
|
Resolution 2.22 Å R-free 0.243 |
| 8BR1 ExoY Nucleotidyl Cyclase domain from Vibrio nigripulchritudo MARTX toxin, bound to Latrunculin-B-ATP-Mg-actin, and 3'-DEOXYADENOSINE-5'-TRIPHOSPHATE and 2 Mg ions Deposited 2022-11-22 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
|
Not recorded | LAB LATRUNCULIN B × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 3 3AT 3'-DEOXYADENOSINE-5'-TRIPHOSPHATE × 1 SO4 SULFATE ION × 1 GOL GLYCEROL × 1 PEO HYDROGEN PEROXIDE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；26 % peg 3350, 26 % Glycerol, 30 mM LiSO4,
0.1M TrisHCl pH8.5,
3 % Dioxane,
|
Resolution 2.04 Å R-free 0.222 |
| 8BR1 ExoY Nucleotidyl Cyclase domain from Vibrio nigripulchritudo MARTX toxin, bound to Latrunculin-B-ATP-Mg-actin, and 3'-DEOXYADENOSINE-5'-TRIPHOSPHATE and 2 Mg ions Deposited 2022-11-22 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
3–377（375 aa）
|
Not recorded | LAB LATRUNCULIN B × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 3 3AT 3'-DEOXYADENOSINE-5'-TRIPHOSPHATE × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；26 % peg 3350, 26 % Glycerol, 30 mM LiSO4,
0.1M TrisHCl pH8.5,
3 % Dioxane,
|
Resolution 2.04 Å R-free 0.222 |
| 8DMX Cryo-EM structure of skeletal muscle alpha-actin Deposited 2022-07-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.37 Å |
| 8F8P Cryo-EM structure of F-actin in the ADP state Deposited 2022-11-22 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Blot force 0
Blot time 2.5 s
|
Resolution 2.26 Å |
| 8F8Q Cryo-EM structure of the CapZ-capped barbed end of F-actin Deposited 2022-11-22 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 6 MG MAGNESIUM ION × 6 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Blot force 0
Blot time 2.5 s
|
Resolution 2.79 Å |
| 8F8R Cryo-EM structure of the free barbed end of F-actin Deposited 2022-11-22 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 7 PDB declaration: heptameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 7 MG MAGNESIUM ION × 7 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Blot force 0
Blot time 2.5 s
|
Resolution 3.30 Å |
| 8F8S Cryo-EM structure of the free pointed end of F-actin Deposited 2022-11-22 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 7 PDB declaration: heptameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 7 MG MAGNESIUM ION × 7 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Blot force 0
Blot time 2.5 s
|
Resolution 2.84 Å |
| 8F8T Cryo-EM structure of the Tropomodulin-capped pointed end of F-actin Deposited 2022-11-22 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 7 MG MAGNESIUM ION × 7 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Blot force 0
Blot time 2.5 s
|
Resolution 3.26 Å |
| 8JO3 Cryo-EM structure of a Legionella effector complexed with actin and AMP Deposited 2023-06-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
1–377（377 aa）
|
Not recorded | AMP ADENOSINE MONOPHOSPHATE × 1 CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.66 Å |
| 8JO4 Cryo-EM structure of a Legionella effector complexed with actin and ATP Deposited 2023-06-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
1–377（377 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 1 CA CALCIUM ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.04 Å |
| 8OF8 Cryo-EM structure of actomyosin-5a-S1 with the full-length lever (nucleotide free) Deposited 2023-03-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain J
3–377（375 aa）
Chain K
3–377（375 aa）
Chain L
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.50 Å |
| 8PVX Structure of the Lifeact13-F-actin complex Deposited 2023-07-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.55 Å |
| 8R9V CryoEM structure of the primed actomyosin-5a complex Deposited 2023-11-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | PO4 PHOSPHATE ION × 1 MG MAGNESIUM ION × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE；F-actin was mixed with myosin-5a (S1 1 IQ motif, residues 1-797, S217A, DDEK 594-597 deletion) that had been pre-incubated with ATP, and vitrified at 10 ms post-mixing
|
Resolution 4.40 Å |
| 8RBF CryoEM structure of the post-powerstroke actomyosin-5a complex Deposited 2023-12-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE；F-actin was mixed with myosin-5a (S1 1 IQ motif, residues 1-797, S217A, DDEK 594-597 deletion) that had been pre-incubated with ATP, and vitrified at 120 ms post-mixing
|
Resolution 4.20 Å |
| 8RU0 Structure of the undecorated barbed end of F-actin. Deposited 2024-01-29 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 3 MG MAGNESIUM ION × 4 PO4 PHOSPHATE ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.1；12 mM HEPES pH 7.1, 100 mM KCl, 2.1 mM MgCl2, 1 mM EGTA, 1 mM TCEP, 0.2 mM ATP
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE；3 seconds, force 0.
|
Resolution 3.08 Å |
| 8RV2 Structure of the formin INF2 bound to the barbed end of F-actin. Deposited 2024-01-31 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 3 MG MAGNESIUM ION × 4 PO4 PHOSPHATE ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.1；12 mM HEPES pH 7.1, 100 mM KCl, 2.1 mM MgCl2, 1 mM EGTA, 1 mM TCEP, 0.2 mM ATP
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE；3 seconds, force 0.
|
Resolution 3.41 Å |
| 8UEE Atomic structure of Salmonella SipA/F-actin complex by cryo-EM Deposited 2023-10-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 11 PDB declaration: undecameric |
Chain F
3–377（375 aa）
Chain H
3–377（375 aa）
Chain I
3–377（375 aa）
Chain J
3–377（375 aa）
Chain K
3–377（375 aa）
Chain L
3–377（375 aa）
Chain M
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 7 MG MAGNESIUM ION × 7 PO4 PHOSPHATE ION × 7 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8；Buffer composition:
25 mM TRIS-H-Cl pH 8.0
2 mM DTT
cryo-EM vitrification conditions
Cryogen ETHANE；3 uL of sample was applied on Lacey grid, then sample was blotted for 3 seconds and plunge-froze in liquid ethane
|
Resolution 3.20 Å |
| 8UXW Arp2/3 branch junction complex, ADP state Deposited 2023-11-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric |
Chain H
1–377（377 aa）
Chain I
1–377（377 aa）
Chain M
1–377（377 aa）
Chain N
1–377（377 aa）
Chain O
1–377（377 aa）
Chain P
1–377（377 aa）
Chain Q
1–377（377 aa）
Chain R
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 9 MG MAGNESIUM ION × 10 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；The samples were incubated on the grid for 50 s and the extra solution was blotted using two Vitrobot filter papers (0.55/20 mm, Grade 595, Ted Pella) for 4 s at 0 blot force. The grids were plunged into liquid ethane at ~180 degrees C with a wait time of 0.5 s.
|
Resolution 2.70 Å |
| 8UXX Arp2/3 branch junction complex, BeFx state Deposited 2023-11-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric |
Chain H
1–377（377 aa）
Chain I
1–377（377 aa）
Chain M
1–377（377 aa）
Chain N
1–377（377 aa）
Chain O
1–377（377 aa）
Chain P
1–377（377 aa）
Chain Q
1–377（377 aa）
Chain R
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 9 MG MAGNESIUM ION × 10 BEF BERYLLIUM TRIFLUORIDE ION × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；The samples were incubated on the grid for 50 s and the extra solution was blotted using two Vitrobot filter papers (0.55/20 mm, Grade 595, Ted Pella) for 4 s at 0 blot force. The grids were plunged into liquid ethane at ~180 degrees C with a wait time of 0.5 s.
|
Resolution 3.20 Å |
| 8UZ0 Straight actin filament from Arp2/3 branch junction sample (ADP) Deposited 2023-11-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 9 PDB declaration: nonameric |
Chain J
6–377（372 aa）
Chain K
6–377（372 aa）
Chain L
6–377（372 aa）
Chain M
6–377（372 aa）
Chain N
6–377（372 aa）
Chain O
6–377（372 aa）
Chain P
6–377（372 aa）
Chain Q
6–377（372 aa）
Chain R
6–377（372 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 9 MG MAGNESIUM ION × 9 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；The samples were incubated on the grid for 50 s and the extra solution was blotted using two Vitrobot filter papers (0.55/20 mm, Grade 595, Ted Pella) for 4 s at 0 blot force. The grids were plunged into liquid ethane at ~180 degrees C with a wait time of 0.5 s.
|
Resolution 2.80 Å |
| 8UZ1 Straight actin filament from Arp2/3 branch junction sample (ADP-BeFx) Deposited 2023-11-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 9 PDB declaration: nonameric |
Chain J
6–377（372 aa）
Chain K
6–377（372 aa）
Chain L
6–377（372 aa）
Chain M
6–377（372 aa）
Chain N
6–377（372 aa）
Chain O
6–377（372 aa）
Chain P
6–377（372 aa）
Chain Q
6–377（372 aa）
Chain R
6–377（372 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 9 MG MAGNESIUM ION × 9 BEF BERYLLIUM TRIFLUORIDE ION × 9 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；The samples were incubated on the grid for 50 s and the extra solution was blotted using two Vitrobot filter papers (0.55/20 mm, Grade 595, Ted Pella) for 4 s at 0 blot force. The grids were plunged into liquid ethane at ~180 degrees C with a wait time of 0.5 s.
|
Resolution 3.60 Å |
| 8VIZ Structure of full-length gelsolin bound to the barbed end of F-actin Deposited 2024-01-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
Chain F
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 CA CALCIUM ION × 14 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.63 Å |
| 8VKH Structure of gelsolin domains G1G3 bound to the barbed end of F-actin Deposited 2024-01-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
Chain F
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 4 CA CALCIUM ION × 14 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.63 Å |
| 8W36 rabbit actin in the absence of potassium Deposited 2024-02-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
7–377（371 aa）
Chain B
7–377（371 aa）
Chain C
7–377（371 aa）
Chain D
7–377（371 aa）
Chain E
7–377（371 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.27 Å |
| 8XDL F-actin-END Deposited 2023-12-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 3 MG MAGNESIUM ION × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.44 Å |
| 8XDM F-actin-MAD Deposited 2023-12-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 3 MG MAGNESIUM ION × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.45 Å |
| 8YAE Cryo-ET structure of huntingtin actin complex Deposited 2024-02-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric |
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
Chain F
3–377（375 aa）
Chain G
3–377（375 aa）
Chain H
3–377（375 aa）
Chain I
3–377（375 aa）
Chain J
3–377（375 aa）
Chain K
3–377（375 aa）
Chain L
3–377（375 aa）
Chain M
3–377（375 aa）
Chain N
3–377（375 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 10.08 Å |
| 8YAO Cryo-ET structure of huntingtin actin dimer complex Deposited 2024-02-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric |
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
Chain F
3–377（375 aa）
Chain G
3–377（375 aa）
Chain H
3–377（375 aa）
Chain I
3–377（375 aa）
Chain J
3–377（375 aa）
Chain K
3–377（375 aa）
Chain L
3–377（375 aa）
Chain M
3–377（375 aa）
Chain N
3–377（375 aa）
Chain b
3–377（375 aa）
Chain c
3–377（375 aa）
Chain d
3–377（375 aa）
Chain e
3–377（375 aa）
Chain f
3–377（375 aa）
Chain g
3–377（375 aa）
Chain h
3–377（375 aa）
Chain i
3–377（375 aa）
Chain j
3–377（375 aa）
Chain k
3–377（375 aa）
Chain l
3–377（375 aa）
Chain m
3–377（375 aa）
Chain n
3–377（375 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 20.80 Å |
| 9AZ4 INF2 at the Barbed End of F-Actin Deposited 2024-03-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
7–377（371 aa）
Chain B
7–377（371 aa）
Chain C
7–377（371 aa）
Chain D
7–377（371 aa）
Chain E
7–377（371 aa）
Chain F
7–377（371 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 6 MG MAGNESIUM ION × 6 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.37 Å |
| 9AZ6 F-actin-Talin(R13-DD) complex Deposited 2024-03-10 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 13 PDB declaration: tridecameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；3.0 ul sample, blotted for 5 s from both sides with filter paper Whatman No.1
|
Resolution 2.98 Å |
| 9AZP INF2 at the Barbed End of F-Actin with Incoming Profilin-Actin Deposited 2024-03-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
7–377（371 aa）
Chain B
7–377（371 aa）
Chain C
7–377（371 aa）
Chain D
7–377（371 aa）
Chain E
7–377（371 aa）
Chain F
7–377（371 aa）
Chain I
7–377（371 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 6 MG MAGNESIUM ION × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.79 Å |
| 9AZQ INF2 at the Barbed End of F-Actin with Incoming Actin Deposited 2024-03-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric |
Chain A
7–377（371 aa）
Chain B
7–377（371 aa）
Chain C
7–377（371 aa）
Chain D
7–377（371 aa）
Chain E
7–377（371 aa）
Chain F
7–377（371 aa）
Chain I
7–377（371 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 7 MG MAGNESIUM ION × 7 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.82 Å |
| 9B03 INF2 in the Middle of F-Actin (Up state) Deposited 2024-03-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
7–377（371 aa）
Chain B
7–377（371 aa）
Chain C
7–377（371 aa）
Chain D
7–377（371 aa）
Chain E
7–377（371 aa）
Chain F
7–377（371 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 6 MG MAGNESIUM ION × 6 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.95 Å |
| 9B0K INF2 in the Middle of F-Actin (Down state) Deposited 2024-03-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
7–377（371 aa）
Chain B
7–377（371 aa）
Chain C
7–377（371 aa）
Chain D
7–377（371 aa）
Chain E
7–377（371 aa）
Chain F
7–377（371 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 6 MG MAGNESIUM ION × 6 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.03 Å |
| 9B27 Dia1 at the Barbed End of F-Actin Deposited 2024-03-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
7–377（371 aa）
Chain B
7–377（371 aa）
Chain C
7–377（371 aa）
Chain D
7–377（371 aa）
Chain E
7–377（371 aa）
Chain F
7–377（371 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 6 MG MAGNESIUM ION × 6 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.51 Å |
| 9B3D mDia1 in the middle of F-actin Deposited 2024-03-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
7–377（371 aa）
Chain B
7–377（371 aa）
Chain C
7–377（371 aa）
Chain D
7–377（371 aa）
Chain E
7–377（371 aa）
Chain F
7–377（371 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 6 MG MAGNESIUM ION × 6 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8；20mM HEPES, 50mM NaCL, 1mM EDTA, 1mM DTT, 0.05% Thesit
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.41 Å |
| 9CFU Cryo-EM structure of myosin-1c bound to F-actin in the ADP-A state Deposited 2024-06-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric |
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 4 MG MAGNESIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 9CFV Cryo-EM structure of delta-NTR myosin-1c bound to F-actin Deposited 2024-06-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric |
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 3 MG MAGNESIUM ION × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 9CFW Cryo-EM structure of myosin-1c bound to F-actin in the ADP-B state Deposited 2024-06-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric |
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 4 MG MAGNESIUM ION × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 9CFX Cryo-EM structure of myosin-1c bound to F-actin in the Rigor state Deposited 2024-06-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric |
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 3 MG MAGNESIUM ION × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 9DFS Structure of novel Myo7a-N isoform (ADP-bound) expressed in sensory hair cells (head domain + first two IQ domains), bound to F-actin Deposited 2024-08-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 4 MG MAGNESIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9DFV Structure of canonical Myo7a-C isoform (ADP-bound) expressed in sensory hair cells (head domain + first two IQ domains), bound to F-actin Deposited 2024-08-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 4 MG MAGNESIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9FJO Structure of the undecorated pointed end of F-actin Deposited 2024-05-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 4 MG MAGNESIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.1；12 mM HEPES pH 7.1, 100 mM KCl, 2.1 mM MgCl2, 1 mM EGTA, 1 mM TCEP, 0.2 mM ATP
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE；3 seconds, force 0.
|
Resolution 3.05 Å |
| 9GOB Structure of the F-tractin-F-actin complex Deposited 2024-09-05 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric |
Chain A
4–377（374 aa）
Chain B
4–377（374 aa）
Chain C
4–377（374 aa）
Chain D
4–377（374 aa）
Chain E
4–377（374 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.20 Å |
| 9HM9 Structure of the optimized F-tractin in complex with F-actin Deposited 2024-12-07 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric |
Chain A
4–377（374 aa）
Chain B
4–377（374 aa）
Chain C
4–377（374 aa）
Chain D
4–377（374 aa）
Chain E
4–377（374 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.40 Å |
| 9KBX CryoEM structure of F-actin bound with GAS2-CH3 domain. Deposited 2024-10-31 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric |
Chain H
7–376（370 aa）
Chain J
7–376（370 aa）
Chain K
7–376（370 aa）
Chain L
7–376（370 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 4 MG MAGNESIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8；50 mM Tris-HCl, pH 8.0, 200 mM KCl, 2 mM MgCl2, 1 mM EGTA, 4 mM DTT.
cryo-EM vitrification conditions
Cryogen ETHANE；The grid was blotted for 4 s at force 10 and plunged into ethane immediately.
|
Resolution 2.80 Å |
| 9NB9 Viral protein DP71L in complex with phosphorylated eIF2alpha (NTD) and protein phosphatase 1A (D64A), stabilized by G-actin/DNAseI Deposited 2025-02-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric |
Chain D
1–377（377 aa）
|
Not recorded | MN MANGANESE (II) ION × 1 CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；3.5 uL volume, -5 blot force, 1.5 blot time
|
Resolution 3.03 Å |
| 9P3D cryo-EM structure of Vibrio effector VopV fragment bound to skeletal alpha F-actin Deposited 2025-06-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric |
Chain Q
6–377（372 aa）
Chain R
6–377（372 aa）
Chain S
6–377（372 aa）
Chain T
6–377（372 aa）
Chain U
6–377（372 aa）
Chain V
6–377（372 aa）
Chain W
6–377（372 aa）
Chain X
6–377（372 aa）
Chain Y
6–377（372 aa）
Chain Z
6–377（372 aa）
Chain a
6–377（372 aa）
|
Not recorded | MG MAGNESIUM ION × 11 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 11 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 9Q7K Pointed end of Cofilin-2 Bound F-actin Deposited 2025-08-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
2–377（376 aa）
Chain B
2–377（376 aa）
Chain C
2–377（376 aa）
Chain D
2–377（376 aa）
Chain E
2–377（376 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.13 Å |
| 9Q7L Barbed end of F-actin and cofilin on sides Deposited 2025-08-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
2–377（376 aa）
Chain B
2–377（376 aa）
Chain C
2–377（376 aa）
Chain D
2–377（376 aa）
Chain E
2–377（376 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.68 Å |
| 9Q7M Barbed end of cofilin actin, cofilin on second-to-last barbed end subunit Deposited 2025-08-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric |
Chain A
2–377（376 aa）
Chain B
2–377（376 aa）
Chain C
2–377（376 aa）
Chain D
2–377（376 aa）
Chain E
2–377（376 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 9Q7N Cofilin barbed end, cofilin on the two barbed end subunits Deposited 2025-08-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
2–377（376 aa）
Chain B
2–377（376 aa）
Chain C
2–377（376 aa）
Chain D
2–377（376 aa）
Chain E
2–377（376 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.48 Å |
| 9Q7O One CAP-1 Bound to the Pointed End of F-actin Deposited 2025-08-24 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric |
Chain A
2–377（376 aa）
Chain B
2–377（376 aa）
Chain C
2–377（376 aa）
Chain D
2–377（376 aa）
Chain E
2–377（376 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.02 Å |
| 9UG2 Severed and capped actin fragment by two G1G3 domains of gelsolin Deposited 2025-04-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric |
Chain C
7–376（370 aa）
Chain D
7–376（370 aa）
Chain E
7–376（370 aa）
Chain F
7–376（370 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 4 MG MAGNESIUM ION × 5 PO4 PHOSPHATE ION × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.89 Å |
| 9XYE Two CAP-1 Bound to the Pointed End of F-actin Deposited 2025-08-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric |
Chain A
2–377（376 aa）
Chain B
2–377（376 aa）
Chain C
2–377（376 aa）
Chain D
2–377（376 aa）
Chain E
2–377（376 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.18 Å |
| 9Y52 One CAP-1 Bound to the Pointed End of Cofilin F-actin Deposited 2025-09-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 11 PDB declaration: undecameric |
Chain A
2–377（376 aa）
Chain B
2–377（376 aa）
Chain C
2–377（376 aa）
Chain D
2–377（376 aa）
Chain E
2–377（376 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.46 Å |
| 9Y9J Two CAP-1 Bound to the Pointed End of Cofilin F-actin Deposited 2025-09-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain A
2–377（376 aa）
Chain B
2–377（376 aa）
Chain C
2–377（376 aa）
Chain D
2–377（376 aa）
Chain E
2–377（376 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.58 Å |
| 9Y9L CP at the barbed end with one cofilin on second-to-last subunit Deposited 2025-09-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 11 PDB declaration: 11-meric |
Chain A
2–377（376 aa）
Chain B
2–377（376 aa）
Chain C
2–377（376 aa）
Chain D
2–377（376 aa）
Chain E
2–377（376 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.06 Å |
| 9Y9M Capping protein bound to the barbed end of cofilactin Deposited 2025-09-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
2–377（376 aa）
Chain B
2–377（376 aa）
Chain C
2–377（376 aa）
Chain D
2–377（376 aa）
Chain E
2–377（376 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.06 Å |
| 9Y9P Cofilactin filament Deposited 2025-09-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
2–377（376 aa）
Chain B
2–377（376 aa）
Chain C
2–377（376 aa）
Chain D
2–377（376 aa）
Chain E
2–377（376 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.06 Å |
| 9YIM Capping protein bound to the barbed end of F-actin Deposited 2025-10-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric |
Chain A
7–377（371 aa）
Chain B
7–377（371 aa）
Chain C
7–377（371 aa）
Chain D
7–377（371 aa）
Chain E
7–377（371 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.62 Å |
293 other PDB entries and 353 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | ACTS_RABIT |
| Isoform | — |
| PDB entities | 1 |
| Chains and sequence ranges | Author chain A； PDBConstruct 1–375； UniProt 3–377 Author chain B； PDBConstruct 1–375； UniProt 3–377 Author chain C； PDBConstruct 1–375； UniProt 3–377 Author chain D； PDBConstruct 1–375； UniProt 3–377 Author chain E； PDBConstruct 1–375； UniProt 3–377 |
