1o1f

MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE

Method: ELECTRON MICROSCOPY D_max: 289.6 Å Quality: EXCELLENT

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	1o1f
沉积日期 deposition_date	2002-11-15
结构标题 title	MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE
关键词 keywords	ACTIN-MYOSIN COMPLEX IN SITU IN MUSCLE, CONTRACTILE PROTEIN; CONTRACTILE PROTEIN
实验方法 method	ELECTRON MICROSCOPY

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	— Å
回转半径 Rg (电子) rg_electron	107.60 Å
零角强度 I(0) i0	16109700000.00
分子量 molecular_weight	1093900.0 kDa
排除体积 excluded_volume	1373200 Å³
包络体积 envelope_volume	2446200 Å³
水化壳体积 shell_volume	206120 Å³
包络直径 envelope_diameter	414.4 Å
壳层 Rg shell_rg	83.00 Å
包络 Rg envelope_rg	106.10 Å
形状 Rg shape_rg	107.60 Å
总 Rg total_rg	107.40 Å
总原子数 total_atoms	76872
残基数 n_residues	9556
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	289.6 Å
Rg (实空间) rg_real	101.90 Å
Rg 误差 (实空间) rg_real_error	1.05 Å
I(0) (实空间) i0_real	1.5360e+10
I(0) 误差 (实空间) i0_real_error	3.1750e+08
Rg (倒空间) rg_reciprocal	101.10 Å
I(0) (倒空间) i0_reciprocal	15810000000.0000
解质量估计 total_estimate	0.9004
解质量评级 solution_quality	EXCELLENT a EXCELLENT solution
P(r) 峰数 n_peaks	1
主峰位置 r_peak_primary	124.1 Å
偏度 Skewness skewness	0.204
峰度 Kurtosis kurtosis	-0.719
角度范围 angular_range	— – 0.0700 Å⁻¹
当前正则化参数 α current_alpha	1.8050
最高正则化参数 α highest_alpha	383600000.0000
实空间数据点数 n_real_points	15
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.009; Oscil: 0.995; Stabil: 0.953; Sysdev: 1.000; Positv: 1.000; Valcen: 0.887; Smooth: 0.000

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (4)

Entity 1 polymer

描述 description	SKELETAL MUSCLE MYOSIN II
分子量 formula_weight	96625.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	4

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A,D,G,J
原子数 atom_count	76872

DAEMAAFGEAAPYLRKSEKERIEAQNKPFDAKSSVFVVHPKQSFVKGTIQSKEGGKVTVKTEGGETLTVKEDQVFSMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNPKVVLAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAASGEKKKEEQSGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVTFQLPAERSYHIFYQIMSNKKPELIDMLLITTNPYDYHYVSEGEITVPSIDDQEELMATDSAIDILGFSADEKTAIYKLTGAVMHYGNLKFKQKQREEQAEPDGTEVADKAAYLMGLNSAELLKALCYPRVGVGNEAVTKGETVSEVHNSVGALAKAVYEKMFLWMVIRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYDEHLGKSNNFQKPKPAKGKAEAHFSLVHYAGTVDYNISGWLEKNKDPLNETVIGLYQKSSVKTLALLFATYGGEAEGGGGKKGGKKKGSSFQTVSALFRENLNKLMANLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRVLYADFKQRYRVLNASAIPEGQFMDSKKASEKLLGGGDVDHTQYAFGHTKVFFKAGLLGLLEEMRDDKLAEIITATQARCRGFLMRVEYRAMVERRESIFCIQYNVRSFMNVKHWPWMKLFFKIKPLLK

Entity 2 polymer

描述 description	SKELETAL MUSCLE MYOSIN II REGULATORY LIGHT CHAIN
分子量 formula_weight	16063.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	4

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	B,E,H,K

FDETEIEDFKEAFTVIDQNADGIIDKDDLRETFAAMGRLNVKNEELDAMIKEASGPINFTVFLTMFGEKLKGADPEDVIMGAFKVLDPDGKGSIKKSFLEELLTTGGGRFTPEEIKNMWAAFPPDVAGNVDYKNICYVITHGEDA

Entity 3 polymer

描述 description	SKELETAL MUSCLE MYOSIN II ESSENTIAL LIGHT CHAIN
分子量 formula_weight	16063.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	4

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	C,F,I,L

SKAAADDFKEAFLLFDRTGDAKITASQVGDIARALGQNPTNAEINKILGNPSKEEMNAAAITFEEFLPMLQAAANNKDQGTFEDFVEGLRVFDKEGNGTVMGAELRHVLATLGEKMTEEEVEELMKGQEDSNGCINYEAFVKHIMSV

Entity 4 polymer

描述 description	SKELETAL MUSCLE ACTIN
分子量 formula_weight	41862.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	14

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	0,1,2,3,4,5,6,7,8,V,W,X,Y,Z

DEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYDEAGPSIVHRKCF

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6. 折叠分类 (SCOP + CATH) 26 domains

SCOP 2.08 (26 domains)

结构域编号 domain_id	d1o1f0_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1f1_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1f2_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1f3_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1f4_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1f5_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1f6_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1f7_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1f8_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1fa_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1fb_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1fc_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1fd_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1fe_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1ff_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1fg_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1fh_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1fi_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1fj_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1fk_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1fl_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1fv_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1fw_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1fx_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1fy_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1fz_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

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7. 引用文献 (4)

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晶胞边长 a length_a	1.000 Å
晶胞边长 b length_b	1.000 Å
晶胞边长 c length_c	1.000 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	1

空间群 space_group	P 1
空间群编号 int_tables_number	1
晶系 crystal_system	—

分辨率 resolution	70.000 Å
最低分辨率 resolution_low	— Å
R 因子 r_factor	—
R_work r_work	—
R_free r_free	—

分辨率 resolution	70.00 Å
分辨率测定方法 resolution_method	DUAL AXIS TILT SERIES ELECTRON TOMOGRAPHY
颗粒数 num_particles	—

fit	/app/data/saxs_computation/o1/1o1f/1o1f.fit
pddf	/app/data/pr_computation/o1/1o1f/1o1f.pr.out
profile	/app/data/saxs_computation/o1/1o1f/1o1f.dat

MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

精修参数 Refinement

冷冻电镜 EM 3D

5. 实体与聚合体信息 (4)

Entity 1 polymer

聚合体 Polymer

Entity 2 polymer

聚合体 Polymer

Entity 3 polymer

聚合体 Polymer

Entity 4 polymer

聚合体 Polymer

6. 折叠分类 (SCOP + CATH) 26 domains

SCOP 2.08 (26 domains)

7. 引用文献 (4)

8. 文件与曲线 (10)

Files (7)

Curves (3)