1o1f

MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE

Method: ELECTRON MICROSCOPY

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1. Protein Identity and Related Structures Protein Identity & Related Structures

SKELETAL MUSCLE MYOSIN II

OrganismNot specified

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 26	SKELETAL MUSCLE MYOSIN II REGULATORY LIGHT CHAIN × 4 （P02609） SKELETAL MUSCLE MYOSIN II ESSENTIAL LIGHT CHAIN × 4 （P02605） SKELETAL MUSCLE ACTIN × 14 （P68135）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	MYSS_CHICK
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–840； UniProt 4–843 Author chain D； PDBConstruct 1–840； UniProt 4–843 Author chain G； PDBConstruct 1–840； UniProt 4–843 Author chain J； PDBConstruct 1–840； UniProt 4–843

SKELETAL MUSCLE MYOSIN II REGULATORY LIGHT CHAIN

OrganismNot specified

UniProt P02609

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 26	SKELETAL MUSCLE MYOSIN II × 4 （P13538） SKELETAL MUSCLE MYOSIN II ESSENTIAL LIGHT CHAIN × 4 （P02605） SKELETAL MUSCLE ACTIN × 14 （P68135）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	MLRS_CHICK
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain B； PDBConstruct 9–144； UniProt 29–164 Author chain E； PDBConstruct 9–144； UniProt 29–164 Author chain H； PDBConstruct 9–144； UniProt 29–164 Author chain K； PDBConstruct 9–144； UniProt 29–164

SKELETAL MUSCLE MYOSIN II ESSENTIAL LIGHT CHAIN

OrganismNot specified

UniProt P02605

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 26	SKELETAL MUSCLE MYOSIN II × 4 （P13538） SKELETAL MUSCLE MYOSIN II REGULATORY LIGHT CHAIN × 4 （P02609） SKELETAL MUSCLE ACTIN × 14 （P68135）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	MLE3_CHICK
Isoform	—
PDB entities	3
Chains and sequence ranges	Author chain C； PDBConstruct 7–147； UniProt 9–149 Author chain F； PDBConstruct 7–147； UniProt 9–149 Author chain I； PDBConstruct 7–147； UniProt 9–149 Author chain L； PDBConstruct 7–147； UniProt 9–149

SKELETAL MUSCLE ACTIN

OrganismNot specified

UniProt P68135

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 26	SKELETAL MUSCLE MYOSIN II × 4 （P13538） SKELETAL MUSCLE MYOSIN II REGULATORY LIGHT CHAIN × 4 （P02609） SKELETAL MUSCLE MYOSIN II ESSENTIAL LIGHT CHAIN × 4 （P02605）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	ACTS_RABIT
Isoform	—
PDB entities	4
Chains and sequence ranges	Author chain 0； PDBConstruct 1–375； UniProt 3–377 Author chain 1； PDBConstruct 1–375； UniProt 3–377 Author chain 2； PDBConstruct 1–375； UniProt 3–377 Author chain 3； PDBConstruct 1–375； UniProt 3–377 Author chain 4； PDBConstruct 1–375； UniProt 3–377 Author chain 5； PDBConstruct 1–375； UniProt 3–377 Author chain 6； PDBConstruct 1–375； UniProt 3–377 Author chain 7； PDBConstruct 1–375； UniProt 3–377 Author chain 8； PDBConstruct 1–375； UniProt 3–377 Author chain V； PDBConstruct 1–375； UniProt 3–377 Author chain W； PDBConstruct 1–375； UniProt 3–377 Author chain X； PDBConstruct 1–375； UniProt 3–377 Author chain Y； PDBConstruct 1–375； UniProt 3–377 Author chain Z； PDBConstruct 1–375； UniProt 3–377

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	1o1f
Deposition date deposition_date	2002-11-15
Structure title title	MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE
Keywords keywords	ACTIN-MYOSIN COMPLEX IN SITU IN MUSCLE, CONTRACTILE PROTEIN; CONTRACTILE PROTEIN
Experimental Method method	ELECTRON MICROSCOPY

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

1o1f__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

1o1f__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

1o1f__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	0.00 Å
Rg (electron density)	107.60 Å
Total Rg	107.40 Å
Atom count	76872
Residues	9556
Excluded volume	1373200 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`1o1f__assembly_1__model_1`	26-meric （26）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (4)

Entity 1 Entity 1 polymer

Description description	SKELETAL MUSCLE MYOSIN II
Molecular weight formula_weight	96625.5 kDa
Source method src_method	nat
Copy count entity_count	4

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,D,G,J
Atom count atom_count	76872

DAEMAAFGEAAPYLRKSEKERIEAQNKPFDAKSSVFVVHPKQSFVKGTIQSKEGGKVTVKTEGGETLTVKEDQVFSMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNPKVVLAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAASGEKKKEEQSGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVTFQLPAERSYHIFYQIMSNKKPELIDMLLITTNPYDYHYVSEGEITVPSIDDQEELMATDSAIDILGFSADEKTAIYKLTGAVMHYGNLKFKQKQREEQAEPDGTEVADKAAYLMGLNSAELLKALCYPRVGVGNEAVTKGETVSEVHNSVGALAKAVYEKMFLWMVIRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYDEHLGKSNNFQKPKPAKGKAEAHFSLVHYAGTVDYNISGWLEKNKDPLNETVIGLYQKSSVKTLALLFATYGGEAEGGGGKKGGKKKGSSFQTVSALFRENLNKLMANLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRVLYADFKQRYRVLNASAIPEGQFMDSKKASEKLLGGGDVDHTQYAFGHTKVFFKAGLLGLLEEMRDDKLAEIITATQARCRGFLMRVEYRAMVERRESIFCIQYNVRSFMNVKHWPWMKLFFKIKPLLK

Entity 2 Entity 2 polymer

Description description	SKELETAL MUSCLE MYOSIN II REGULATORY LIGHT CHAIN
Molecular weight formula_weight	16063.0 kDa
Source method src_method	nat
Copy count entity_count	4

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	B,E,H,K

FDETEIEDFKEAFTVIDQNADGIIDKDDLRETFAAMGRLNVKNEELDAMIKEASGPINFTVFLTMFGEKLKGADPEDVIMGAFKVLDPDGKGSIKKSFLEELLTTGGGRFTPEEIKNMWAAFPPDVAGNVDYKNICYVITHGEDA

Entity 3 Entity 3 polymer

Description description	SKELETAL MUSCLE MYOSIN II ESSENTIAL LIGHT CHAIN
Molecular weight formula_weight	16063.9 kDa
Source method src_method	nat
Copy count entity_count	4

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	C,F,I,L

SKAAADDFKEAFLLFDRTGDAKITASQVGDIARALGQNPTNAEINKILGNPSKEEMNAAAITFEEFLPMLQAAANNKDQGTFEDFVEGLRVFDKEGNGTVMGAELRHVLATLGEKMTEEEVEELMKGQEDSNGCINYEAFVKHIMSV

Entity 4 Entity 4 polymer

Description description	SKELETAL MUSCLE ACTIN
Molecular weight formula_weight	41862.6 kDa
Source method src_method	nat
Copy count entity_count	14

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	0,1,2,3,4,5,6,7,8,V,W,X,Y,Z

DEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYDEAGPSIVHRKCF

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6. Fold Classification (SCOP + CATH) 26 domains

SCOP 2.08 (26 domains)

Domain ID domain_id	d1o1f0_
Class class	i — Low resolution protein structures
Fold Fold fold	i.15 — Muscle protein complexes
Superfamily Superfamily superfamily	i.15.1 — Muscle protein complexes
Family Family family	i.15.1.1 — Muscle protein complexes

Domain ID domain_id	d1o1f1_
Class class	i — Low resolution protein structures
Fold Fold fold	i.15 — Muscle protein complexes
Superfamily Superfamily superfamily	i.15.1 — Muscle protein complexes
Family Family family	i.15.1.1 — Muscle protein complexes

Domain ID domain_id	d1o1f2_
Class class	i — Low resolution protein structures
Fold Fold fold	i.15 — Muscle protein complexes
Superfamily Superfamily superfamily	i.15.1 — Muscle protein complexes
Family Family family	i.15.1.1 — Muscle protein complexes

Domain ID domain_id	d1o1f3_
Class class	i — Low resolution protein structures
Fold Fold fold	i.15 — Muscle protein complexes
Superfamily Superfamily superfamily	i.15.1 — Muscle protein complexes
Family Family family	i.15.1.1 — Muscle protein complexes

Domain ID domain_id	d1o1f4_
Class class	i — Low resolution protein structures
Fold Fold fold	i.15 — Muscle protein complexes
Superfamily Superfamily superfamily	i.15.1 — Muscle protein complexes
Family Family family	i.15.1.1 — Muscle protein complexes

Domain ID domain_id	d1o1f5_
Class class	i — Low resolution protein structures
Fold Fold fold	i.15 — Muscle protein complexes
Superfamily Superfamily superfamily	i.15.1 — Muscle protein complexes
Family Family family	i.15.1.1 — Muscle protein complexes

Domain ID domain_id	d1o1f6_
Class class	i — Low resolution protein structures
Fold Fold fold	i.15 — Muscle protein complexes
Superfamily Superfamily superfamily	i.15.1 — Muscle protein complexes
Family Family family	i.15.1.1 — Muscle protein complexes

Domain ID domain_id	d1o1f7_
Class class	i — Low resolution protein structures
Fold Fold fold	i.15 — Muscle protein complexes
Superfamily Superfamily superfamily	i.15.1 — Muscle protein complexes
Family Family family	i.15.1.1 — Muscle protein complexes

Domain ID domain_id	d1o1f8_
Class class	i — Low resolution protein structures
Fold Fold fold	i.15 — Muscle protein complexes
Superfamily Superfamily superfamily	i.15.1 — Muscle protein complexes
Family Family family	i.15.1.1 — Muscle protein complexes

Domain ID domain_id	d1o1fa_
Class class	i — Low resolution protein structures
Fold Fold fold	i.15 — Muscle protein complexes
Superfamily Superfamily superfamily	i.15.1 — Muscle protein complexes
Family Family family	i.15.1.1 — Muscle protein complexes

Domain ID domain_id	d1o1fb_
Class class	i — Low resolution protein structures
Fold Fold fold	i.15 — Muscle protein complexes
Superfamily Superfamily superfamily	i.15.1 — Muscle protein complexes
Family Family family	i.15.1.1 — Muscle protein complexes

Domain ID domain_id	d1o1fc_
Class class	i — Low resolution protein structures
Fold Fold fold	i.15 — Muscle protein complexes
Superfamily Superfamily superfamily	i.15.1 — Muscle protein complexes
Family Family family	i.15.1.1 — Muscle protein complexes

Domain ID domain_id	d1o1fd_
Class class	i — Low resolution protein structures
Fold Fold fold	i.15 — Muscle protein complexes
Superfamily Superfamily superfamily	i.15.1 — Muscle protein complexes
Family Family family	i.15.1.1 — Muscle protein complexes

Domain ID domain_id	d1o1fe_
Class class	i — Low resolution protein structures
Fold Fold fold	i.15 — Muscle protein complexes
Superfamily Superfamily superfamily	i.15.1 — Muscle protein complexes
Family Family family	i.15.1.1 — Muscle protein complexes

Domain ID domain_id	d1o1ff_
Class class	i — Low resolution protein structures
Fold Fold fold	i.15 — Muscle protein complexes
Superfamily Superfamily superfamily	i.15.1 — Muscle protein complexes
Family Family family	i.15.1.1 — Muscle protein complexes

Domain ID domain_id	d1o1fg_
Class class	i — Low resolution protein structures
Fold Fold fold	i.15 — Muscle protein complexes
Superfamily Superfamily superfamily	i.15.1 — Muscle protein complexes
Family Family family	i.15.1.1 — Muscle protein complexes

Domain ID domain_id	d1o1fh_
Class class	i — Low resolution protein structures
Fold Fold fold	i.15 — Muscle protein complexes
Superfamily Superfamily superfamily	i.15.1 — Muscle protein complexes
Family Family family	i.15.1.1 — Muscle protein complexes

Domain ID domain_id	d1o1fi_
Class class	i — Low resolution protein structures
Fold Fold fold	i.15 — Muscle protein complexes
Superfamily Superfamily superfamily	i.15.1 — Muscle protein complexes
Family Family family	i.15.1.1 — Muscle protein complexes

Domain ID domain_id	d1o1fj_
Class class	i — Low resolution protein structures
Fold Fold fold	i.15 — Muscle protein complexes
Superfamily Superfamily superfamily	i.15.1 — Muscle protein complexes
Family Family family	i.15.1.1 — Muscle protein complexes

Domain ID domain_id	d1o1fk_
Class class	i — Low resolution protein structures
Fold Fold fold	i.15 — Muscle protein complexes
Superfamily Superfamily superfamily	i.15.1 — Muscle protein complexes
Family Family family	i.15.1.1 — Muscle protein complexes

Domain ID domain_id	d1o1fl_
Class class	i — Low resolution protein structures
Fold Fold fold	i.15 — Muscle protein complexes
Superfamily Superfamily superfamily	i.15.1 — Muscle protein complexes
Family Family family	i.15.1.1 — Muscle protein complexes

Domain ID domain_id	d1o1fv_
Class class	i — Low resolution protein structures
Fold Fold fold	i.15 — Muscle protein complexes
Superfamily Superfamily superfamily	i.15.1 — Muscle protein complexes
Family Family family	i.15.1.1 — Muscle protein complexes

Domain ID domain_id	d1o1fw_
Class class	i — Low resolution protein structures
Fold Fold fold	i.15 — Muscle protein complexes
Superfamily Superfamily superfamily	i.15.1 — Muscle protein complexes
Family Family family	i.15.1.1 — Muscle protein complexes

Domain ID domain_id	d1o1fx_
Class class	i — Low resolution protein structures
Fold Fold fold	i.15 — Muscle protein complexes
Superfamily Superfamily superfamily	i.15.1 — Muscle protein complexes
Family Family family	i.15.1.1 — Muscle protein complexes

Domain ID domain_id	d1o1fy_
Class class	i — Low resolution protein structures
Fold Fold fold	i.15 — Muscle protein complexes
Superfamily Superfamily superfamily	i.15.1 — Muscle protein complexes
Family Family family	i.15.1.1 — Muscle protein complexes

Domain ID domain_id	d1o1fz_
Class class	i — Low resolution protein structures
Fold Fold fold	i.15 — Muscle protein complexes
Superfamily Superfamily superfamily	i.15.1 — Muscle protein complexes
Family Family family	i.15.1.1 — Muscle protein complexes

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Unit-cell length a length_a	1.000 Å
Unit-cell length b length_b	1.000 Å
Unit-cell length c length_c	1.000 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	1

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	—

Resolution resolution	70.000 Å
Low-resolution limit resolution_low	— Å
R factor r_factor	—
R_work r_work	—
R_free r_free	—

Resolution resolution	70.00 Å
Resolution测定Method resolution_method	DUAL AXIS TILT SERIES ELECTRON TOMOGRAPHY
颗粒数 num_particles	—

MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE

1. Protein Identity and Related Structures Protein Identity & Related Structures

SKELETAL MUSCLE MYOSIN II

State in the Current Structure

Other States of the Same Protein in the Database

SKELETAL MUSCLE MYOSIN II REGULATORY LIGHT CHAIN

State in the Current Structure

Other States of the Same Protein in the Database

SKELETAL MUSCLE MYOSIN II ESSENTIAL LIGHT CHAIN

State in the Current Structure

Other States of the Same Protein in the Database

SKELETAL MUSCLE ACTIN

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

1o1f__assembly_1__model_1

1o1f__assembly_1__model_1 | I(q)

1o1f__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

冷冻电镜 EM 3D

5. Entities and Polymers Entities & Polymers (4)

Entity 1 Entity 1 polymer

Polymer Polymer

Entity 2 Entity 2 polymer

Polymer Polymer

Entity 3 Entity 3 polymer

Polymer Polymer

Entity 4 Entity 4 polymer

Polymer Polymer

6. Fold Classification (SCOP + CATH) 26 domains

SCOP 2.08 (26 domains)

7. Citations (4)

MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE

1. Protein Identity and Related Structures Protein Identity & Related Structures

SKELETAL MUSCLE MYOSIN II

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

SKELETAL MUSCLE MYOSIN II REGULATORY LIGHT CHAIN

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

SKELETAL MUSCLE MYOSIN II ESSENTIAL LIGHT CHAIN

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

SKELETAL MUSCLE ACTIN

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

1o1f__assembly_1__model_1

1o1f__assembly_1__model_1 | I(q)

1o1f__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

冷冻电镜 EM 3D

5. Entities and Polymers Entities & Polymers (4)

Entity 1 Entity 1 polymer

Polymer Polymer

Entity 2 Entity 2 polymer

Polymer Polymer

Entity 3 Entity 3 polymer

Polymer Polymer

Entity 4 Entity 4 polymer

Polymer Polymer

6. Fold Classification (SCOP + CATH) 26 domains

SCOP 2.08 (26 domains)

7. Citations (4)