1o1e

MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE

Method: ELECTRON MICROSCOPY D_max: 303.3 Å Quality: EXCELLENT

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	1o1e
沉积日期 deposition_date	2002-11-19
结构标题 title	MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE
关键词 keywords	ACTIN-MYOSIN COMPLEX IN SITU IN MUSCLE, CONTRACTILE PROTEIN; CONTRACTILE PROTEIN
实验方法 method	ELECTRON MICROSCOPY

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	— Å
回转半径 Rg (电子) rg_electron	116.80 Å
零角强度 I(0) i0	24314900000.00
分子量 molecular_weight	1351100.0 kDa
排除体积 excluded_volume	1697700 Å³
包络体积 envelope_volume	3293300 Å³
水化壳体积 shell_volume	246490 Å³
包络直径 envelope_diameter	423.9 Å
壳层 Rg shell_rg	91.70 Å
包络 Rg envelope_rg	112.90 Å
形状 Rg shape_rg	116.80 Å
总 Rg total_rg	116.60 Å
总原子数 total_atoms	94966
残基数 n_residues	11730
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	303.3 Å
Rg (实空间) rg_real	110.60 Å
Rg 误差 (实空间) rg_real_error	1.11 Å
I(0) (实空间) i0_real	2.3300e+10
I(0) 误差 (实空间) i0_real_error	4.7240e+08
Rg (倒空间) rg_reciprocal	110.00 Å
I(0) (倒空间) i0_reciprocal	23760000000.0000
解质量估计 total_estimate	0.9140
解质量评级 solution_quality	EXCELLENT a EXCELLENT solution
P(r) 峰数 n_peaks	1
主峰位置 r_peak_primary	116.7 Å
偏度 Skewness skewness	0.180
峰度 Kurtosis kurtosis	-0.714
角度范围 angular_range	— – 0.0650 Å⁻¹
当前正则化参数 α current_alpha	0.6463
最高正则化参数 α highest_alpha	543000000.0000
实空间数据点数 n_real_points	14
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.003; Oscil: 0.994; Stabil: 0.981; Sysdev: 1.000; Positv: 1.000; Valcen: 0.963; Smooth: 0.000

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (4)

Entity 1 polymer

描述 description	SKELETAL MUSCLE MYOSIN II
分子量 formula_weight	96625.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	6

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A,D,G,J,M,P
原子数 atom_count	94966

DAEMAAFGEAAPYLRKSEKERIEAQNKPFDAKSSVFVVHPKQSFVKGTIQSKEGGKVTVKTEGGETLTVKEDQVFSMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNPKVVLAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAASGEKKKEEQSGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVTFQLPAERSYHIFYQIMSNKKPELIDMLLITTNPYDYHYVSEGEITVPSIDDQEELMATDSAIDILGFSADEKTAIYKLTGAVMHYGNLKFKQKQREEQAEPDGTEVADKAAYLMGLNSAELLKALCYPRVGVGNEAVTKGETVSEVHNSVGALAKAVYEKMFLWMVIRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYDEHLGKSNNFQKPKPAKGKAEAHFSLVHYAGTVDYNISGWLEKNKDPLNETVIGLYQKSSVKTLALLFATYGGEAEGGGGKKGGKKKGSSFQTVSALFRENLNKLMANLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRVLYADFKQRYRVLNASAIPEGQFMDSKKASEKLLGGGDVDHTQYAFGHTKVFFKAGLLGLLEEMRDDKLAEIITATQARCRGFLMRVEYRAMVERRESIFCIQYNVRSFMNVKHWPWMKLFFKIKPLLK

Entity 2 polymer

描述 description	SKELETAL MUSCLE MYOSIN II REGULATORY LIGHT CHAIN
分子量 formula_weight	16063.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	6

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	B,E,H,K,N,Q

FDETEIEDFKEAFTVIDQNADGIIDKDDLRETFAAMGRLNVKNEELDAMIKEASGPINFTVFLTMFGEKLKGADPEDVIMGAFKVLDPDGKGSIKKSFLEELLTTGGGRFTPEEIKNMWAAFPPDVAGNVDYKNICYVITHGEDA

Entity 3 polymer

描述 description	SKELETAL MUSCLE MYOSIN II ESSENTIAL LIGHT CHAIN
分子量 formula_weight	16063.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	6

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	C,F,I,L,O,R

SKAAADDFKEAFLLFDRTGDAKITASQVGDIARALGQNPTNAEINKILGNPSKEEMNAAAITFEEFLPMLQAAANNKDQGTFEDFVEGLRVFDKEGNGTVMGAELRHVLATLGEKMTEEEVEELMKGQEDSNGCINYEAFVKHIMSV

Entity 4 polymer

描述 description	SKELETAL MUSCLE ACTIN
分子量 formula_weight	41862.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	14

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	1,2,3,4,5,6,7,8,9,V,W,X,Y,Z

DEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYDEAGPSIVHRKCF

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6. 折叠分类 (SCOP + CATH) 32 domains

SCOP 2.08 (32 domains)

结构域编号 domain_id	d1o1e1_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1e2_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1e3_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1e4_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1e5_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1e6_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1e7_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1e8_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1e9_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1ea_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1eb_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1ec_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1ed_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1ee_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1ef_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1eg_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1eh_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1ei_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1ej_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1ek_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1el_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1em_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1en_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1eo_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1ep_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1eq_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1er_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1ev_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1ew_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1ex_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1ey_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

结构域编号 domain_id	d1o1ez_
类 Class class	i — Low resolution protein structures
折叠类型 Fold fold	i.15 — Muscle protein complexes
超家族 Superfamily superfamily	i.15.1 — Muscle protein complexes
家族 Family family	i.15.1.1 — Muscle protein complexes

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7. 引用文献 (4)

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晶胞边长 a length_a	1.000 Å
晶胞边长 b length_b	1.000 Å
晶胞边长 c length_c	1.000 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	1

空间群 space_group	P 1
空间群编号 int_tables_number	1
晶系 crystal_system	—

分辨率 resolution	70.000 Å
最低分辨率 resolution_low	— Å
R 因子 r_factor	—
R_work r_work	—
R_free r_free	—

分辨率 resolution	70.00 Å
分辨率测定方法 resolution_method	DUAL AXIS TILT SERIES ELECTRON TOMOGRAPHY
颗粒数 num_particles	—

fit	/app/data/saxs_computation/o1/1o1e/1o1e.fit
pddf	/app/data/pr_computation/o1/1o1e/1o1e.pr.out
profile	/app/data/saxs_computation/o1/1o1e/1o1e.dat

MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

精修参数 Refinement

冷冻电镜 EM 3D

5. 实体与聚合体信息 (4)

Entity 1 polymer

聚合体 Polymer

Entity 2 polymer

聚合体 Polymer

Entity 3 polymer

聚合体 Polymer

Entity 4 polymer

聚合体 Polymer

6. 折叠分类 (SCOP + CATH) 32 domains

SCOP 2.08 (32 domains)

7. 引用文献 (4)

8. 文件与曲线 (10)

Files (7)

Curves (3)