Actin, alpha skeletal muscle
OrganismNot specified
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain A； UniProt 3–377 | Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 | FIBER DIFFRACTION mmCIF provides none of the parsed experimental conditions | Resolution 3.30 Å |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 2ZWH | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1EQY COMPLEX BETWEEN RABBIT MUSCLE ALPHA-ACTIN: HUMAN GELSOLIN DOMAIN 1 Deposited 2000-04-06 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 6.6；298 K；PEG 6000, sodium chloride, adenosine triphosphate, calcium, magnesium, sodium azide, pH 6.6, Vapor Diffusion, temperature 298.0K
|
Resolution 2.30 Å R-free 0.280 |
| 1ESV COMPLEX BETWEEN LATRUNCULIN A:RABBIT MUSCLE ALPHA ACTIN:HUMAN GELSOLIN DOMAIN 1 Deposited 2000-04-11 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 LAR LATRUNCULIN A × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 6.6；298 K；PEG 6000, sodium Chloride, adenosine triphosphate, calcium, magnesium, sodium azide, pH 6.6, VAPOR DIFFUSION, temperature 298.0K
|
Resolution 2.00 Å R-free 0.286 |
| 1IJJ THE X-RAY CRYSTAL STRUCTURE OF THE COMPLEX BETWEEN RABBIT SKELETAL MUSCLE ACTIN AND LATRUNCULIN A AT 2.85 A RESOLUTION Deposited 2001-04-26 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–377（377 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 LAR LATRUNCULIN A × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；298 K；ammonium sulfate, magnesium chloride, pH 6.8,
VAPOR DIFFUSION, HANGING DROP at 298 K
|
Resolution 2.85 Å R-free 0.309 |
| 1IJJ THE X-RAY CRYSTAL STRUCTURE OF THE COMPLEX BETWEEN RABBIT SKELETAL MUSCLE ACTIN AND LATRUNCULIN A AT 2.85 A RESOLUTION Deposited 2001-04-26 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–377（377 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 LAR LATRUNCULIN A × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；298 K；ammonium sulfate, magnesium chloride, pH 6.8,
VAPOR DIFFUSION, HANGING DROP at 298 K
|
Resolution 2.85 Å R-free 0.309 |
| 1J6Z UNCOMPLEXED ACTIN Deposited 2001-05-15 | Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 RHO TETRAMETHYLRHODAMINE-5-MALEIMIDE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；PEG 2000 MONOMETHYLETHER 22%, CALCIUM ACETATE 200mM, TRIS 10mM (pH 7), pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.54 Å R-free 0.223 |
| 1KXP CRYSTAL STRUCTURE OF HUMAN VITAMIN D-BINDING PROTEIN IN COMPLEX WITH SKELETAL ACTIN Deposited 2002-02-01 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.6；277 K；Peg 8K 12%, Magnesium Acetate 200mM, Sodium Cacodylate 100 mM, Glycerol 20%, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.10 Å R-free 0.236 |
| 1LCU Polylysine Induces an Antiparallel Actin Dimer that Nucleates Filament Assembly: Crystal Structure at 3.5 A Resolution Deposited 2002-04-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
7–377（371 aa）
Chain B
7–377（371 aa）
|
Not recorded | CA CALCIUM ION × 4 CL CHLORIDE ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 LAR LATRUNCULIN A × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；Ammonium sulfate, MgCl2, Imidazole, ATP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.50 Å R-free 0.266 |
| 1LOT CRYSTAL STRUCTURE OF THE COMPLEX OF ACTIN WITH VITAMIN D-BINDING PROTEIN Deposited 2002-05-07 | Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | GOL GLYCEROL × 6 CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；290 K；PEG8000, cacodylate, calcium acetate, glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K
|
Resolution 2.50 Å R-free 0.279 |
| 1M8Q Molecular Models of Averaged Rigor Crossbridges from Tomograms of Insect Flight Muscle Deposited 2002-07-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 26 PDB declaration: 26-meric |
Chain 0
1–375（375 aa）
Chain 1
1–375（375 aa）
Chain 2
1–375（375 aa）
Chain 3
1–375（375 aa）
Chain 4
1–375（375 aa）
Chain 5
1–375（375 aa）
Chain 7
1–375（375 aa）
Chain 8
1–375（375 aa）
Chain 9
1–375（375 aa）
Chain V
1–375（375 aa）
Chain W
1–375（375 aa）
Chain X
1–375（375 aa）
Chain Y
1–375（375 aa）
Chain Z
1–375（375 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM vitrification conditions
No vitrification. Samples were viewed at room temperature.
|
Resolution 70.00 Å |
| 1MA9 Crystal structure of the complex of human vitamin D binding protein and rabbit muscle actin Deposited 2002-08-02 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
3–372（370 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.3；277 K；PEG 8000, magnesium acetate, sodium cacodylate, glycerol, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.40 Å R-free 0.250 |
| 1MVW MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE Deposited 2002-09-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric |
Chain 1
3–377（375 aa）
Chain 2
3–377（375 aa）
Chain 3
3–377（375 aa）
Chain 4
3–377（375 aa）
Chain 5
3–377（375 aa）
Chain 6
3–377（375 aa）
Chain 7
3–377（375 aa）
Chain 8
3–377（375 aa）
Chain 9
3–377（375 aa）
Chain V
3–377（375 aa）
Chain W
3–377（375 aa）
Chain X
3–377（375 aa）
Chain Y
3–377（375 aa）
Chain Z
3–377（375 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM vitrification conditions
NO VITRIFICATION. SAMPLES WERE VIEWED AT ROOM TEMPERATURE.
|
Resolution 70.00 Å |
| 1NWK CRYSTAL STRUCTURE OF MONOMERIC ACTIN IN THE ATP STATE Deposited 2003-02-06 | Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 4 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 RHO TETRAMETHYLRHODAMINE-5-MALEIMIDE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；PEG 3350, CaCl2, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 393K
|
Resolution 1.85 Å R-free 0.226 |
| 1O18 MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE Deposited 2002-11-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric |
Chain 1
3–377（375 aa）
Chain 2
3–377（375 aa）
Chain 3
3–377（375 aa）
Chain 4
3–377（375 aa）
Chain 5
3–377（375 aa）
Chain 6
3–377（375 aa）
Chain 7
3–377（375 aa）
Chain 8
3–377（375 aa）
Chain 9
3–377（375 aa）
Chain V
3–377（375 aa）
Chain W
3–377（375 aa）
Chain X
3–377（375 aa）
Chain Y
3–377（375 aa）
Chain Z
3–377（375 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM vitrification conditions
NO VITRIFICATION. SAMPLES WERE VIEWED AT ROOM TEMPERATURE.
|
Resolution 70.00 Å |
| 1O19 MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE Deposited 2002-11-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric |
Chain 1
3–377（375 aa）
Chain 2
3–377（375 aa）
Chain 3
3–377（375 aa）
Chain 4
3–377（375 aa）
Chain 5
3–377（375 aa）
Chain 6
3–377（375 aa）
Chain 7
3–377（375 aa）
Chain 8
3–377（375 aa）
Chain 9
3–377（375 aa）
Chain V
3–377（375 aa）
Chain W
3–377（375 aa）
Chain X
3–377（375 aa）
Chain Y
3–377（375 aa）
Chain Z
3–377（375 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM vitrification conditions
NO VITRIFICATION. SAMPLES WERE VIEWED AT ROOM TEMPERATURE.
|
Resolution 70.00 Å |
| 1O1A MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE Deposited 2002-11-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric |
Chain 1
3–377（375 aa）
Chain 2
3–377（375 aa）
Chain 3
3–377（375 aa）
Chain 4
3–377（375 aa）
Chain 5
3–377（375 aa）
Chain 6
3–377（375 aa）
Chain 7
3–377（375 aa）
Chain 8
3–377（375 aa）
Chain 9
3–377（375 aa）
Chain V
3–377（375 aa）
Chain W
3–377（375 aa）
Chain X
3–377（375 aa）
Chain Y
3–377（375 aa）
Chain Z
3–377（375 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM vitrification conditions
NO VITRIFICATION. SAMPLES WERE VIEWED AT ROOM TEMPERATURE.
|
Resolution 70.00 Å |
| 1O1B MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE Deposited 2002-11-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 26 PDB declaration: 26-meric |
Chain 0
3–377（375 aa）
Chain 1
3–377（375 aa）
Chain 2
3–377（375 aa）
Chain 3
3–377（375 aa）
Chain 4
3–377（375 aa）
Chain 5
3–377（375 aa）
Chain 7
3–377（375 aa）
Chain 8
3–377（375 aa）
Chain 9
3–377（375 aa）
Chain V
3–377（375 aa）
Chain W
3–377（375 aa）
Chain X
3–377（375 aa）
Chain Y
3–377（375 aa）
Chain Z
3–377（375 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM vitrification conditions
NO VITRIFICATION. SAMPLES WERE VIEWED AT ROOM TEMPERATURE.
|
Resolution 70.00 Å |
| 1O1C MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE Deposited 2002-11-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 29 PDB declaration: 29-meric |
Chain 0
3–377（375 aa）
Chain 1
3–377（375 aa）
Chain 2
3–377（375 aa）
Chain 3
3–377（375 aa）
Chain 4
3–377（375 aa）
Chain 5
3–377（375 aa）
Chain 7
3–377（375 aa）
Chain 8
3–377（375 aa）
Chain 9
3–377（375 aa）
Chain V
3–377（375 aa）
Chain W
3–377（375 aa）
Chain X
3–377（375 aa）
Chain Y
3–377（375 aa）
Chain Z
3–377（375 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM vitrification conditions
NO VITRIFICATION. SAMPLES WERE VIEWED AT ROOM TEMPERATURE.
|
Resolution 70.00 Å |
| 1O1D MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE Deposited 2002-11-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric |
Chain 0
3–377（375 aa）
Chain 1
3–377（375 aa）
Chain 2
3–377（375 aa）
Chain 3
3–377（375 aa）
Chain 4
3–377（375 aa）
Chain 5
3–377（375 aa）
Chain 7
3–377（375 aa）
Chain 8
3–377（375 aa）
Chain 9
3–377（375 aa）
Chain V
3–377（375 aa）
Chain W
3–377（375 aa）
Chain X
3–377（375 aa）
Chain Y
3–377（375 aa）
Chain Z
3–377（375 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM vitrification conditions
NO VITRIFICATION. SAMPLES WERE VIEWED AT ROOM TEMPERATURE.
|
Resolution 70.00 Å |
| 1O1E MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE Deposited 2002-11-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric |
Chain 1
3–377（375 aa）
Chain 2
3–377（375 aa）
Chain 3
3–377（375 aa）
Chain 4
3–377（375 aa）
Chain 5
3–377（375 aa）
Chain 6
3–377（375 aa）
Chain 7
3–377（375 aa）
Chain 8
3–377（375 aa）
Chain 9
3–377（375 aa）
Chain V
3–377（375 aa）
Chain W
3–377（375 aa）
Chain X
3–377（375 aa）
Chain Y
3–377（375 aa）
Chain Z
3–377（375 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM vitrification conditions
NO VITRIFICATION. SAMPLES WERE VIEWED AT ROOM TEMPERATURE.
|
Resolution 70.00 Å |
| 1O1F MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE Deposited 2002-11-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 26 PDB declaration: 26-meric |
Chain 0
3–377（375 aa）
Chain 1
3–377（375 aa）
Chain 2
3–377（375 aa）
Chain 3
3–377（375 aa）
Chain 4
3–377（375 aa）
Chain 5
3–377（375 aa）
Chain 6
3–377（375 aa）
Chain 7
3–377（375 aa）
Chain 8
3–377（375 aa）
Chain V
3–377（375 aa）
Chain W
3–377（375 aa）
Chain X
3–377（375 aa）
Chain Y
3–377（375 aa）
Chain Z
3–377（375 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM vitrification conditions
NO VITRIFICATION. SAMPLES WERE VIEWED AT ROOM TEMPERATURE.
|
Resolution 70.00 Å |
| 1O1G MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE Deposited 2002-11-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric |
Chain 1
3–377（375 aa）
Chain 2
3–377（375 aa）
Chain 3
3–377（375 aa）
Chain 4
3–377（375 aa）
Chain 5
3–377（375 aa）
Chain 6
3–377（375 aa）
Chain 7
3–377（375 aa）
Chain 8
3–377（375 aa）
Chain 9
3–377（375 aa）
Chain V
3–377（375 aa）
Chain W
3–377（375 aa）
Chain X
3–377（375 aa）
Chain Y
3–377（375 aa）
Chain Z
3–377（375 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM vitrification conditions
NO VITRIFICATION. SAMPLES WERE VIEWED AT ROOM TEMPERATURE.
|
Resolution 70.00 Å |
| 1P8Z Complex Between Rabbit Muscle alpha-Actin: Human Gelsolin Residues Val26-Glu156 Deposited 2003-05-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–377（377 aa）
|
Not recorded | CD CADMIUM ION × 3 CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；293 K；0.1M Sodium Acetate, 10 mM Cadmium Chloride, 12.5% (v/v) PEG 400, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.60 Å R-free 0.263 |
| 1P8Z Complex Between Rabbit Muscle alpha-Actin: Human Gelsolin Residues Val26-Glu156 Deposited 2003-05-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–377（377 aa）
|
Not recorded | CD CADMIUM ION × 6 CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；293 K；0.1M Sodium Acetate, 10 mM Cadmium Chloride, 12.5% (v/v) PEG 400, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.60 Å R-free 0.263 |
| 1QZ5 Structure of rabbit actin in complex with kabiramide C Deposited 2003-09-15 | Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 KAB KABIRAMIDE C × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
Batch；pH 5.5；293 K；Crystals of the actin-kabiramide C complex were grown by small-scale batch by mixing equal volumes of the complex and 100 mM MES, 18% (w/v) polyethylene glycol 1500, 12% (w/v) 1,6-hexanediol, 100 mM CaCl2, 1 mM sodium azide, 1 mM TCEP, pH 5.5, Batch, temperature 293K
|
Resolution 1.45 Å R-free 0.187 |
| 1QZ6 Structure of rabbit actin in complex with jaspisamide A Deposited 2003-09-15 | Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 JAS JASPISAMIDE A × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
Batch；pH 7；293 K；Crystals were grown by small-scale batch by mixing equal volumes of the complex (10 mg/ml protein) and 100 mM bis-tris propane, 15% (w/v) dimethyl polyethylene glycol 5000, 25 mM CaCl2, 1 mM sodium azide, 1 mM TCEP, pH 7.0, Batch, temperature 293K
|
Resolution 1.60 Å R-free 0.197 |
| 1RDW Actin Crystal Dynamics: Structural Implications for F-actin Nucleation, Polymerization and Branching Mediated by the Anti-parallel Dimer Deposited 2003-11-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain X
3–377（375 aa）
Fragment：ACtin
|
Not recorded | MG MAGNESIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 LAR LATRUNCULIN A × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.7；MgCl2, NH4SO4, pH 6.7, VAPOR DIFFUSION, HANGING DROP
|
Resolution 2.30 Å R-free 0.224 |
| 1RFQ Actin Crystal Dynamics: Structural Implications for F-actin Nucleation, Polymerization and Branching Mediated by the Anti-parallel Dimer Deposited 2003-11-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
Fragment：actin
Chain B
3–377（375 aa）
Fragment：actin
|
Not recorded | MG MAGNESIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 LAR LATRUNCULIN A × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.7；MgCl2, NH4SO4, pH 6.7, VAPOR DIFFUSION, HANGING DROP
|
Resolution 3.00 Å R-free 0.261 |
| 1RGI Crystal structure of gelsolin domains G1-G3 bound to actin Deposited 2003-11-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–377（377 aa）
|
Not recorded | CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；277 K；2% PEG 8000, 100 mM Sodium acetate, 1 mM CaCl2, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 3.00 Å R-free 0.258 |
| 1S22 Absolute Stereochemistry of Ulapualide A Deposited 2004-01-07 | Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–375（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 ULA ULAPUALIDE A × 1 EDO 1,2-ETHANEDIOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
Batch；pH 6；293 K；100 mM MES, 15% methyl ether poly(ethylene glycol) 5000, 75 mM CaCl2, 1 mM sodium azide, 1 mM TCEP, pH 6.0, Batch, temperature 293K
|
Resolution 1.60 Å R-free 0.179 |
| 1SQK CRYSTAL STRUCTURE OF CIBOULOT IN COMPLEX WITH SKELETAL ACTIN Deposited 2004-03-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–377（377 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 LAR LATRUNCULIN A × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；PEG 8000 20%, MAGNESIUM ACETATE 200MM, SODIUM CACODYLATE 100 MM, pH 6.50, VAPOR DIFFUSION, HANGING DROP
|
Resolution 2.50 Å R-free 0.278 |
| 1WUA The structure of Aplyronine A-actin complex Deposited 2004-12-03 | Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 AP8 (8R,9R,10R,11R,14S,18S,20S,24S)-24-{(1R,2S,3R,6R,7R,8R,9S,10E)-8-(ACETYLOXY)-6-[(N,N-DIMETHYLALANYL)OXY]-11-[FORMYL(MET HYL)AMINO]-2-HYDROXY-1,3,7,9-TETRAMETHYLUNDEC-10-ENYL}-10-HYDROXY-14,20-DIMETHOXY-9,11,15,18-TETRAMETHYL-2-OXOOXACYCLOTE TRACOSA-3,5,15,21-TETRAEN-8-YL N,N,O-TRIMETHYLSERINATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；PEG8000 20%(w/v) 0.1M sodium cacodylate , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.45 Å R-free 0.176 |
| 1Y64 Bni1p Formin Homology 2 Domain complexed with ATP-actin Deposited 2004-12-03 | Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.6；298 K；potassium bromide, hepes, DTT, Tris, ATP, calcium chloride, ethylene glycol, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.05 Å R-free 0.313 |
| 1YXQ Crystal structure of actin in complex with swinholide A Deposited 2005-02-22 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 EDO 1,2-ETHANEDIOL × 2 SWI SWINHOLIDE A × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
small-scale batch；pH 8.5；277 K；dimethyl PEG 5000, HEPPS, MgCl2, TCEP, NaN3, pH 8.5, small-scale batch, temperature 277K
|
Resolution 2.01 Å R-free 0.219 |
| 2A3Z Ternary complex of the WH2 domain of WASP with Actin-DNAse I Deposited 2005-06-27 | Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GOL GLYCEROL × 3 MG MAGNESIUM ION × 1 FMT FORMIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.2；293 K；sodium formate, PEG2000 MME, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.08 Å R-free 0.210 |
| 2A40 Ternary complex of the WH2 domain of WAVE with Actin-DNAse I Deposited 2005-06-27 | Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GOL GLYCEROL × 2 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.6；293 K；sodium formate, PEG3350, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.80 Å R-free 0.215 |
| 2A40 Ternary complex of the WH2 domain of WAVE with Actin-DNAse I Deposited 2005-06-27 | Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Other combination Heteromer；Protein × 3 PDB declaration: trimeric |
Chain D
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.6；293 K；sodium formate, PEG3350, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.80 Å R-free 0.215 |
| 2A40 Ternary complex of the WH2 domain of WAVE with Actin-DNAse I Deposited 2005-06-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 3 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain A
3–377（375 aa）
Chain D
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 GOL GLYCEROL × 2 MG MAGNESIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.6；293 K；sodium formate, PEG3350, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.80 Å R-free 0.215 |
| 2A41 Ternary complex of the WH2 Domain of WIP with Actin-DNAse I Deposited 2005-06-27 | Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 FMT FORMIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；sodium formate, PEG3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.60 Å R-free 0.219 |
| 2A42 Actin-DNAse I Complex Deposited 2005-06-27 | Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GOL GLYCEROL × 3 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；sodium formate, PEG3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.85 Å R-free 0.199 |
| 2A5X Crystal Structure of a Cross-linked Actin Dimer Deposited 2005-07-01 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
|
Not recorded | CA CALCIUM ION × 4 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 LAR LATRUNCULIN A × 2 NSB N,N,N-TRIMETHYL-3-SULFOPROPAN-1-AMINIUM × 2 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.7；293 K；35% MPD, 100 mM sodium acetate pH 4.7, 20 mM calcium chloride, non-detergent sulfo-betaine, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.49 Å R-free 0.250 |
| 2ASM Structure of Rabbit Actin In Complex With Reidispongiolide A Deposited 2005-08-23 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–377（375 aa）
|
Not recorded | CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 RGA REIDISPONGIOLIDE A × 1 EDO 1,2-ETHANEDIOL × 5 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；100mM Na/MES/Acetate, pH 5.5, 7% methyl ether poly(ethylene glycol) 5000, 100mM CaCl2, 1mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.60 Å R-free 0.189 |
| 2ASO Structure of Rabbit Actin In Complex With Sphinxolide B Deposited 2005-08-23 | Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 SPX SPHINXOLIDE B × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；100mM Na/MES/Acetate, pH 5.5, 12% methyl ether poly(ethylene glycol) 5000, 100mM CaCl2, 1mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.70 Å R-free 0.213 |
| 2ASP Structure of Rabbit Actin In Complex With Reidispongiolide C Deposited 2005-08-23 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–377（375 aa）
|
Not recorded | CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 RGC REIDISPONGIOLIDE C × 1 EDO 1,2-ETHANEDIOL × 5 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；50mM MES, pH 6.5, 12% methyl ether poly(ethylene glycol) 5000, 40mM MgCl2, 10% ethylene glycol, 1mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.64 Å R-free 0.193 |
| 2D1K Ternary complex of the WH2 domain of mim with actin-dnase I Deposited 2005-08-26 | Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；sodium formate, PEG3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.50 Å R-free 0.284 |
| 2FF3 Crystal structure of Gelsolin domain 1:N-wasp V2 motif hybrid in complex with actin Deposited 2005-12-19 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain B
3–377（375 aa）
|
Not recorded | CA CALCIUM ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
MICROBATCH；pH 6.5；293.15 K；5% PEG 8000, 0.1M sodium acetate, 10mM calcium chloride, pH 6.5, microbatch, temperature 293.15K
|
Resolution 2.00 Å R-free 0.250 |
| 2FF6 Crystal structure of Gelsolin domain 1:ciboulot domain 2 hybrid in complex with actin Deposited 2005-12-19 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
3–377（375 aa）
|
Not recorded | CA CALCIUM ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293.15 K；7% PEG 3000, 0.1M Hepes, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K
|
Resolution 2.05 Å R-free 0.237 |
| 2FXU X-ray Structure of Bistramide A- Actin Complex at 1.35 A resolution. Deposited 2006-02-06 | Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 BID BISTRAMIDE A × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 6；298 K；The bistramide A- actin complex was mixed with the crystallization buffer in 1:1 ratio. The crystallization buffer is 100 mM MES (ph 6.0), 24% (w/v) PEG1500, 70 mM CaCl2, 1mM NaN3, 1mM TCEP., VAPOR DIFFUSION, temperature 298K
|
Resolution 1.35 Å R-free 0.201 |
| 2GWJ SpvB ADP-ribosylated actin: hexagonal crystal form Deposited 2006-05-04 | Different construct Different ligand/ion Different experimental method Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
7–377（371 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.90 Å R-free 0.184 |
| 2GWK SpvB ADP-ribosylated actin: orthorhombic crystal form Deposited 2006-05-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
7–377（371 aa）
Chain B
7–377（371 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.00 Å R-free 0.211 |
| 2HMP Uncomplexed actin cleaved with protease ECP32 Deposited 2006-07-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | SR STRONTIUM ION × 7 SPD SPERMIDINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 EDO 1,2-ETHANEDIOL × 9 211 2,2',2''-NITRILOTRIETHANOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.75；277 K；1:1 mixture of 5-7.5 mg/ml protein solution containing 0.5 mM ATP and
precipitant solution (40 mM SrCl2, 10% ethylene glycol, 13-15%
dimethyl polyethylene glycol 5000, 50 mM triethanolamine, 10 mM
spermidine, pH 7.75) equilibrated against 0.5 ml precipitant., VAPOR
DIFFUSION, HANGING DROP, temperature 277K, VAPOR DIFFUSION, HANGING DROP
|
Resolution 1.90 Å R-free 0.215 |
| 2PAV Ternary complex of Profilin-Actin with the Last Poly-Pro of Human VASP Deposited 2007-03-27 | Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；277.15 K；200 mM sodium formate, 20% PEG 3350, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K
|
Resolution 1.80 Å R-free 0.208 |
| 2PBD Ternary complex of profilin-actin with the poly-PRO-GAB domain of VASP* Deposited 2007-03-28 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
1–377（377 aa）
Fragment：residues 1-377
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；277.15 K；150mM DL-malic acid pH 7.0, 18% PEG 3350, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K
|
Resolution 1.50 Å R-free 0.190 |
| 2Q0R Structure of Pectenotoxin-2 Bound to Actin Deposited 2007-05-22 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–377（375 aa）
|
Not recorded | CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 PXT PECTENOTOXIN-2 × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；100 mM Na/MES/acetate, pH 5.5, 15% methyl ether poly(ethylene glycol) 5000, 10% hexanediol, 30 mM CaCl2, and 1 mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.70 Å R-free 0.204 |
| 2Q0U Structure of Pectenotoxin-2 and Latrunculin B Bound to Actin Deposited 2007-05-22 | Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 LAB LATRUNCULIN B × 1 PXT PECTENOTOXIN-2 × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；100 mM Na/MES/acetate, pH 5.5, 15% methyl ether poly(ethylene glycol) 5000, 10% hexanediol, 100 mM CaCl2, and 1 mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.45 Å R-free 0.181 |
| 2Q1N Actin Dimer Cross-linked Between Residues 41 and 374 Deposited 2007-05-25 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
|
Not recorded | CA CALCIUM ION × 4 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 LAR LATRUNCULIN A × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；298 K；30% MPD, 0.1M sodium acetate, 0.02 M calcium chloride, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.70 Å R-free 0.277 |
| 2Q1N Actin Dimer Cross-linked Between Residues 41 and 374 Deposited 2007-05-25 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain B
3–377（375 aa）
|
Not recorded | CA CALCIUM ION × 4 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 LAR LATRUNCULIN A × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；298 K；30% MPD, 0.1M sodium acetate, 0.02 M calcium chloride, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.70 Å R-free 0.277 |
| 2Q31 Actin Dimer Cross-linked Between Residues 41 and 374 and proteolytically cleaved by subtilisin between residues 47 and 48. Deposited 2007-05-29 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
|
Not recorded | CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 LAR LATRUNCULIN A × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；298 K；30% MPD, 0.1 M sodium acetate, 0.01M calcium chloride, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.70 Å R-free 0.288 |
| 2Q31 Actin Dimer Cross-linked Between Residues 41 and 374 and proteolytically cleaved by subtilisin between residues 47 and 48. Deposited 2007-05-29 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain B
3–377（375 aa）
|
Not recorded | CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 LAR LATRUNCULIN A × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；298 K；30% MPD, 0.1 M sodium acetate, 0.01M calcium chloride, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.70 Å R-free 0.288 |
| 2Q36 Actin Dimer Cross-linked between Residues 191 and 374 and complexed with Kabiramide C Deposited 2007-05-29 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
|
Not recorded | CA CALCIUM ION × 2 SO4 SULFATE ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 KAB KABIRAMIDE C × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；298 K；30% PEG4000, 0.1 M Tris, 0.2 M lithium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.50 Å R-free 0.223 |
| 2Q97 Complex of mammalian actin with toxofilin from toxoplasma gondii Deposited 2007-06-12 | Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 4.6；277 K；8% PEG 4000, 10% GLYCEROL, 0.1M SODIUM ACETATE, pH 4.6, VAPOR DIFFUSION, temperature 277K
|
Resolution 2.50 Å R-free 0.284 |
| 2Q97 Complex of mammalian actin with toxofilin from toxoplasma gondii Deposited 2007-06-12 | Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 4.6；277 K；8% PEG 4000, 10% GLYCEROL, 0.1M SODIUM ACETATE, pH 4.6, VAPOR DIFFUSION, temperature 277K
|
Resolution 2.50 Å R-free 0.284 |
| 2V51 Structure of MAL-RPEL1 complexed to actin Deposited 2008-10-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain B
1–377（377 aa）
Chain D
1–377（377 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 2 CA CALCIUM ION × 2 LAB LATRUNCULIN B × 2 PEG DI(HYDROXYETHYL)ETHER × 2 SCN THIOCYANATE ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.5；pH 6.5
|
Resolution 2.35 Å R-free 0.248 |
| 2V52 Structure of MAL-RPEL2 complexed to G-actin Deposited 2008-10-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
1–377（377 aa）
|
Not recorded | LAB LATRUNCULIN B × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GOL GLYCEROL × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.45 Å R-free 0.188 |
| 2VCP Crystal structure of N-Wasp VC domain in complex with skeletal actin Deposited 2007-09-26 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
Fragment：RESIDUES 3-377
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7；277 K；VAPOR DIFFUSION METHOD (4 C) PROTEIN SOLUTION: 0.18MM ACTIN, 0.36MM N-WASP PEPTIDE, 5MM TRIS.HCL PH7.0, ATP 0.2MM, CACL2 0.02MM, TCEP 20MM, NAN3 0.01%. RESERVOIR: 10.2% (V/V) TACSIMATE, 13.2%(W/V)PEG 8000, 100MM HEPES PH7.0
|
Resolution 3.20 Å R-free 0.331 |
| 2VCP Crystal structure of N-Wasp VC domain in complex with skeletal actin Deposited 2007-09-26 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
3–377（375 aa）
Fragment：RESIDUES 3-377
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7；277 K；VAPOR DIFFUSION METHOD (4 C) PROTEIN SOLUTION: 0.18MM ACTIN, 0.36MM N-WASP PEPTIDE, 5MM TRIS.HCL PH7.0, ATP 0.2MM, CACL2 0.02MM, TCEP 20MM, NAN3 0.01%. RESERVOIR: 10.2% (V/V) TACSIMATE, 13.2%(W/V)PEG 8000, 100MM HEPES PH7.0
|
Resolution 3.20 Å R-free 0.331 |
| 2VYP Rabbit-muscle G-actin in complex with myxobacterial rhizopodin Deposited 2008-07-25 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–377（377 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1 RH9 RHIZOPODIN × 1 HEZ HEXANE-1,6-DIOL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.6；100 MM MES PH 6.6 14% (W/W) PEG1500 12% (W/W) 1,6-HEXANEDIOL 100 MM CACL2 1 MM DTT 10 MM BETAINE-HYDROCHLORIDE
|
Resolution 2.35 Å R-free 0.241 |
| 2VYP Rabbit-muscle G-actin in complex with myxobacterial rhizopodin Deposited 2008-07-25 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–377（377 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.6；100 MM MES PH 6.6 14% (W/W) PEG1500 12% (W/W) 1,6-HEXANEDIOL 100 MM CACL2 1 MM DTT 10 MM BETAINE-HYDROCHLORIDE
|
Resolution 2.35 Å R-free 0.241 |
| 2W49 ISOMETRICALLY CONTRACTING INSECT ASYNCHRONOUS FLIGHT MUSCLE Deposited 2008-11-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 36 PDB declaration: 36-meric |
Chain D
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain E
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain F
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain G
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain H
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain I
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain J
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain K
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain L
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain M
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain N
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain O
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain P
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain Q
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain R
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain S
3–374（372 aa）
Fragment：RESIDUES 3-374
|
Not recorded | CA CALCIUM ION × 16 |
ELECTRON MICROSCOPY
cryo-EM buffer
20 MM MOPS BUFFER, 5 MM NAN3, AND MGCL2, ATP, CACL2, AND EGTA IN VARYING MILLIMOLAR CONCENTRATIONS；20 MM MOPS BUFFER, 5 MM NAN3, AND MGCL2, ATP, CACL2, AND EGTA IN VARYING MILLIMOLAR CONCENTRATIONS
cryo-EM vitrification conditions
Cryogen HELIUM；SMASH AGAINST A LIQUID HELIUM COOLED GOLD COATED COPPER MIRROR
|
Resolution 35.00 Å |
| 2W4U Isometrically contracting insect asynchronous flight muscle quick frozen after a length step Deposited 2008-12-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 36 PDB declaration: 36-meric |
Chain D
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain E
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain F
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain G
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain H
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain I
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain J
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain K
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain L
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain M
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain N
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain O
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain P
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain Q
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain R
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain S
3–374（372 aa）
Fragment：RESIDUES 3-374
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
20 MM MOPS BUFFER, 5 MM NAN3, AND MGCL2, ATP, CACL2, AND EGTA IN VARYING MILLIMOLAR CONCENTRATIONS；20 MM MOPS BUFFER, 5 MM NAN3, AND MGCL2, ATP, CACL2, AND EGTA IN VARYING MILLIMOLAR CONCENTRATIONS
cryo-EM vitrification conditions
Cryogen HELIUM；SMASH AGAINST A LIQUID HELIUM COOLED GOLD COATED COPPER MIRROR
|
Resolution 35.00 Å |
| 2Y83 Actin filament pointed end Deposited 2011-02-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain O
3–377（375 aa）
Chain P
3–377（375 aa）
Chain Q
3–377（375 aa）
Chain R
3–377（375 aa）
Chain S
3–377（375 aa）
Chain T
3–377（375 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 6 CA CALCIUM ION × 6 |
ELECTRON MICROSCOPY
cryo-EM buffer
50 MM NACL, 10 MM SODIUM PHOSPHATE BUFFER PH 7.4 3 MM MGCL2, 0.005% (W/V) NAN3, 0.7 MM DTT.；pH 7.4；50 MM NACL, 10 MM SODIUM PHOSPHATE BUFFER PH 7.4 3 MM MGCL2, 0.005% (W/V) NAN3, 0.7 MM DTT.
cryo-EM vitrification conditions
Cryogen ETHANE；VITRIFICATION 1 - CRYOGEN- ETHANE, HUMIDITY- 90%, TEMPERATURE- 4 DEGREES CELSIUS. METHOD- BLOT FOR 3 SECONDS BEFORE PLUNGING.
|
Resolution 22.90 Å |
| 2YJE Oligomeric assembly of actin bound to MRTF-A Deposited 2011-05-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
|
Not recorded | LAB LATRUNCULIN B × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8.25；0.1 M BTP PH 8.25, 20.5% PEG 3350, 0.2 M SODIUM NITRATE.
|
Resolution 3.10 Å R-free 0.280 |
| 2YJF Oligomeric assembly of actin bound to MRTF-A Deposited 2011-05-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
|
Not recorded | LAB LATRUNCULIN B × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5.3；pH 5.3
|
Resolution 3.50 Å R-free 0.272 |
| 2YJF Oligomeric assembly of actin bound to MRTF-A Deposited 2011-05-19 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
1–377（377 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5.3；pH 5.3
|
Resolution 3.50 Å R-free 0.272 |
| 2YJF Oligomeric assembly of actin bound to MRTF-A Deposited 2011-05-19 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain E
1–377（377 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5.3；pH 5.3
|
Resolution 3.50 Å R-free 0.272 |
| 3B5U Actin filament model from extended form of acromsomal bundle in the Limulus sperm Deposited 2007-10-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 14 PDB declaration: tetradecameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
Chain H
1–377（377 aa）
Chain I
1–377（377 aa）
Chain J
1–377（377 aa）
Chain K
1–377（377 aa）
Chain L
1–377（377 aa）
Chain M
1–377（377 aa）
Chain N
1–377（377 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 7.4；see J Mol Biol, 221, 711-725 (1991)
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 9.50 Å |
| 3BUZ Crystal structure of ia-bTAD-actin complex Deposited 2008-01-04 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
3–377（375 aa）
|
Not recorded | TAD BETA-METHYLENE-THIAZOLE-4-CARBOXYAMIDE-ADENINE DINUCLEOTIDE × 1 CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 LAR LATRUNCULIN A × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；20% PEG1000, 0.1M MES(pH6.5), VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.81 Å R-free 0.298 |
| 3CJB Actin dimer cross-linked by V. cholerae MARTX toxin and complexed with Gelsolin-segment 1 Deposited 2008-03-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–377（377 aa）
|
Not recorded | CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；10% Peg MME 5000, 5% Tacsimate, 0.1 M Hepes, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.21 Å R-free 0.269 |
| 3CJC Actin dimer cross-linked by V. cholerae MARTX toxin and complexed with DNase I and Gelsolin-segment 1 Deposited 2008-03-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
1–377（377 aa）
|
Not recorded | CA CALCIUM ION × 2 SO4 SULFATE ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；1.5 M (NH4)SO4, 0.1 M Bis-Tris, 0.1 M NaCl, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.90 Å R-free 0.278 |
| 3DAW Structure of the actin-depolymerizing factor homology domain in complex with actin Deposited 2008-05-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–377（377 aa）
|
Not recorded | CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；10mM Tris pH 7.5, 50mM NaCl, 0.2mM ATP, 0.2mM DTT, 0.2mM CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.55 Å R-free 0.279 |
| 3FFK Crystal structure of human Gelsolin domains G1-G3 bound to Actin Deposited 2008-12-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
1–377（377 aa）
|
Not recorded | CA CALCIUM ION × 5 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；297 K；9% PEG 4000, 100 mM Sodium Acetate, 100 mM Calcium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 297K
|
Resolution 3.00 Å R-free 0.273 |
| 3FFK Crystal structure of human Gelsolin domains G1-G3 bound to Actin Deposited 2008-12-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain E
1–377（377 aa）
|
Not recorded | CA CALCIUM ION × 5 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；297 K；9% PEG 4000, 100 mM Sodium Acetate, 100 mM Calcium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 297K
|
Resolution 3.00 Å R-free 0.273 |
| 3G37 Cryo-EM structure of actin filament in the presence of phosphate Deposited 2009-02-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: dodecameric |
Chain O
3–377（375 aa）
Chain P
3–377（375 aa）
Chain Q
3–377（375 aa）
Chain R
3–377（375 aa）
Chain S
3–377（375 aa）
Chain T
3–377（375 aa）
Chain U
3–377（375 aa）
Chain V
3–377（375 aa）
Chain W
3–377（375 aa）
Chain X
3–377（375 aa）
Chain Y
3–377（375 aa）
Chain Z
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 12 PO4 PHOSPHATE ION × 36 MG MAGNESIUM ION × 72 |
ELECTRON MICROSCOPY
cryo-EM buffer
phosphate buffer；pH 7.4；phosphate buffer
cryo-EM vitrification conditions
77 K；Cryogen ETHANE
|
Resolution 6.00 Å |
| 3HBT The structure of native G-actin Deposited 2009-05-05 | Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 1 CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；295 K；2M ammonium sulfate, 100mM Tris-HCl, pH8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.70 Å R-free 0.257 |
| 3J4K Cryo-EM structures of the actin:tropomyosin filament reveal the mechanism for the transition from C- to M-state Deposited 2013-08-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
70 mM NaCl, 3 mM MgCl2, 0.2 mM EGTA, 5 mM NaH2PO4, 5 mM PIPES buffer；pH 7.5；70 mM NaCl, 3 mM MgCl2, 0.2 mM EGTA, 5 mM NaH2PO4, 5 mM PIPES buffer
cryo-EM vitrification conditions
3 second blot；Cryogen ETHANE；3 second blot before plunging into liquid ethane (FEI Vitrobot Mark IV)
|
Resolution 8.00 Å |
| 3J8A Structure of the F-actin-tropomyosin complex Deposited 2014-10-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
5 mM Tris-HCl, pH 7.5, 1 mM DTT, 100 mM KCl, 2 mM MgCl2；pH 7.5；5 mM Tris-HCl, pH 7.5, 1 mM DTT, 100 mM KCl, 2 mM MgCl2
cryo-EM vitrification conditions
Sample was applied to grid, incubated for 10 seconds, and manually blotted for 3 seconds from the backside with filter paper.；106 K；Cryogen ETHANE；Sample was applied to grid, incubated for 10 seconds, and manually blotted for 3 seconds from the backside with filter paper before plunging into liquid ethane (GATAN CRYOPLUNGE 3)
|
Resolution 3.70 Å R-free 0.271 |
| 3J8I Near-Atomic Resolution for One State of F-Actin Deposited 2014-11-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
Chain H
1–377（377 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM vitrification conditions
Cryogen ETHANE；Plunged into liquid ethane (FEI VITROBOT MARK IV)
|
Resolution 4.70 Å |
| 3J8J Tilted state of actin, T1 Deposited 2014-11-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 11 PDB declaration: undecameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
Chain H
1–377（377 aa）
Chain I
1–377（377 aa）
Chain J
1–377（377 aa）
Chain K
1–377（377 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM vitrification conditions
Cryogen ETHANE；Plunged into liquid ethane
|
Resolution 12.00 Å |
| 3J8K Tilted state of actin, T2 Deposited 2014-11-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
Chain H
1–377（377 aa）
Chain I
1–377（377 aa）
Chain J
1–377（377 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 12.00 Å |
| 3JBI MDFF model of the vinculin tail domain bound to F-actin Deposited 2015-09-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
|
Not recorded | MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
50 mM KCl, 1 mM MgCl2, 1 mM EGTA, 10 mM imidazole；pH 7；50 mM KCl, 1 mM MgCl2, 1 mM EGTA, 10 mM imidazole
cryo-EM vitrification conditions
3 microliters of 0.3 micromolar actin was applied to the grid and incubated for 60 seconds at 25 degrees C. 3 microliters of 10 micromolar Vt was then applied and incubated for 60 seconds. 3 microliters of solution was removed, then an additional 3 microliters of Vt applied. After 60 seconds, 3 microliters of solution was removed, then the grid was blotted for 2 seconds before plunging.；Cryogen ETHANE；3 microliters of 0.3 micromolar actin was applied to the grid and incubated for 60 seconds at 25 degrees C. 3 microliters of 10 micromolar Vt was then applied and incubated for 60 seconds. 3 microliters of solution was removed, then an additional 3 microliters of Vt applied. After 60 seconds, 3 microliters of solution was removed, then the grid was blotted for 2 seconds before plunging into liquid ethane (LEICA EM GP).
|
Resolution 8.50 Å |
| 3JBJ Cryo-EM reconstruction of F-actin Deposited 2015-09-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
|
Not recorded | MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
50 mM KCl, 1 mM MgCl2, 1 mM EGTA, 10 mM imidazole；pH 7；50 mM KCl, 1 mM MgCl2, 1 mM EGTA, 10 mM imidazole
cryo-EM vitrification conditions
3 microliters of 0.3 micromolar actin was applied to the grid and incubated for 60 seconds at 25 degrees C. The grid was blotted for 2 seconds before plunging.；Cryogen ETHANE；3 microliters of 0.3 micromolar actin was applied to the grid and incubated for 60 seconds at 25 degrees C. The grid was blotted for 2 seconds before plunging into liquid ethane (LEICA EM GP).
|
Resolution 7.60 Å |
| 3JBK Cryo-EM reconstruction of the metavinculin-actin interface Deposited 2015-09-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
|
Not recorded | MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
50 mM KCl, 1 mM MgCl2, 1 mM EGTA, 10 mM imidazole；pH 7；50 mM KCl, 1 mM MgCl2, 1 mM EGTA, 10 mM imidazole
cryo-EM vitrification conditions
3 microliters of 0.3 micromolar actin was applied to the grid and incubated for 60 seconds at 25 degrees C. 3 microliters of 10 micromolar MVt was then applied and incubated for 60 seconds. 3 microliters of solution was removed, then an additional 3 microliters of MVt applied. After 60 seconds, 3 microliters of solution was removed, then the grid was blotted for 2 seconds before plunging.；Cryogen ETHANE；3 microliters of 0.3 micromolar actin was applied to the grid and incubated for 60 seconds at 25 degrees C. 3 microliters of 10 micromolar MVt was then applied and incubated for 60 seconds. 3 microliters of solution was removed, then an additional 3 microliters of MVt applied. After 60 seconds, 3 microliters of solution was removed, then the grid was blotted for 2 seconds before plunging into liquid ethane (LEICA EM GP).
|
Resolution 8.20 Å |
| 3M1F Crosslinked complex of actin with first W domain of Vibrio parahaemolyticus VopL Deposited 2010-03-04 | Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.1；298 K；0.2 N Lithium Nitrate, 20% polyethylene glycol 3350, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
Resolution 2.89 Å R-free 0.265 |
| 3M3N Structure of a Longitudinal Actin Dimer Assembled by Tandem W Domains Deposited 2010-03-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ATP ADENOSINE-5'-TRIPHOSPHATE × 2 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 10；300 K；100 mM CAPS pH 10.0, and 24% PEG 3350, 100 mM RbCl, VAPOR DIFFUSION, HANGING DROP, temperature 300.0K
|
Resolution 7.00 Å |
| 3M6G Crystal structure of actin in complex with lobophorolide Deposited 2010-03-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
3–373（371 aa）
Fragment：UNP residues 3-373
Chain B
3–373（371 aa）
Fragment：UNP residues 3-373
|
Not recorded | MG MAGNESIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 LO3 (1S,3S,4S,5S,7R,8S,9R,12E,14E,16R,17R,19R)-16-hydroxy-9-{(1S,2S,3S)-2-hydroxy-5-[(2S,4R,6S)-4-methoxy-6-methyltetrahydro-2H-pyran-2-yl]-1,3-dimethylpentyl}-3,5,7,17-tetramethoxy-8,14-dimethyl-11H-spiro[10,23-dioxabicyclo[17.3.1]tricosa-12,14,20-triene-4,2'-oxiran]-11-one × 2 CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；0.1M MES pH 6.0, 6% methyl
ether poly(ethylene glycol) 5000, 0.1M CaCl2, 1mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.00 Å R-free 0.227 |
| 3M6G Crystal structure of actin in complex with lobophorolide Deposited 2010-03-15 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–373（371 aa）
Fragment：UNP residues 3-373
|
Not recorded | MG MAGNESIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 LO3 (1S,3S,4S,5S,7R,8S,9R,12E,14E,16R,17R,19R)-16-hydroxy-9-{(1S,2S,3S)-2-hydroxy-5-[(2S,4R,6S)-4-methoxy-6-methyltetrahydro-2H-pyran-2-yl]-1,3-dimethylpentyl}-3,5,7,17-tetramethoxy-8,14-dimethyl-11H-spiro[10,23-dioxabicyclo[17.3.1]tricosa-12,14,20-triene-4,2'-oxiran]-11-one × 1 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；0.1M MES pH 6.0, 6% methyl
ether poly(ethylene glycol) 5000, 0.1M CaCl2, 1mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.00 Å R-free 0.227 |
| 3M6G Crystal structure of actin in complex with lobophorolide Deposited 2010-03-15 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
3–373（371 aa）
Fragment：UNP residues 3-373
|
Not recorded | MG MAGNESIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 LO3 (1S,3S,4S,5S,7R,8S,9R,12E,14E,16R,17R,19R)-16-hydroxy-9-{(1S,2S,3S)-2-hydroxy-5-[(2S,4R,6S)-4-methoxy-6-methyltetrahydro-2H-pyran-2-yl]-1,3-dimethylpentyl}-3,5,7,17-tetramethoxy-8,14-dimethyl-11H-spiro[10,23-dioxabicyclo[17.3.1]tricosa-12,14,20-triene-4,2'-oxiran]-11-one × 1 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；0.1M MES pH 6.0, 6% methyl
ether poly(ethylene glycol) 5000, 0.1M CaCl2, 1mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.00 Å R-free 0.227 |
| 3MFP Atomic model of F-actin based on a 6.6 angstrom resolution cryoEM map Deposited 2010-04-03 | Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.60 Å |
| 3MFP Atomic model of F-actin based on a 6.6 angstrom resolution cryoEM map Deposited 2010-04-03 | Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.60 Å |
| 3MFP Atomic model of F-actin based on a 6.6 angstrom resolution cryoEM map Deposited 2010-04-03 | Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.60 Å |
| 3MN5 Structures of actin-bound WH2 domains of Spire and the implication for filament nucleation Deposited 2010-04-21 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
|
Not recorded | CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 LAB LATRUNCULIN B × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.9；277 K；0.2 Magnesium formate pH 5.9
20% PEG 3350
, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 1.50 Å R-free 0.216 |
| 3SJH Crystal Structure of a chimera containing the N-terminal domain (residues 8-29) of drosophila Ciboulot and the C-terminal domain (residues 18-44) of bovine Thymosin-beta4, bound to G-actin-ATP-Latrunculin A Deposited 2011-06-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
Fragment：UNP residues 3-377
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 LAR LATRUNCULIN A × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
hanging drop；pH 6.5；298 K；22% PEG3350, 0.2M MgAcetate pH6.5, 0.45M Guanidine HCl, 1% Dioxane, hanging drop, temperature 298K
|
Resolution 1.75 Å R-free 0.199 |
| 3TPQ Crystal structure of wild-type MAL RPEL domain in complex with five G-actins Deposited 2011-09-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 5 CA CALCIUM ION × 5 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 5.3；293 K；50mM citrate, 0.1M ammonium sulfate, 0.1mM CaCl2, 0.1mM ATP, 10% PEG8000, pH 5.3, VAPOR DIFFUSION, temperature 293K
|
Resolution 3.45 Å R-free 0.273 |
| 3TU5 Actin complex with Gelsolin Segment 1 fused to Cobl segment Deposited 2011-09-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–377（377 aa）
|
Not recorded | CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；298 K；10% (w/v) polyethyleneglycol 20000, 2% (v/v) dioxane, and 0.1M bicine, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.00 Å R-free 0.204 |
| 3U8X Crystal Structure of a chimera containing the N-terminal domain (residues 8-29) of drosophila Ciboulot and the C-terminal domain (residues 18-44) of bovine Thymosin-beta4, bound to G-actin-ATP Deposited 2011-10-17 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
hanging drop；pH 6.5；298 K；20% PEG3350, 0.2M MgAcetate pH6.5, 0.45M Guanidine HCl, 1% Dioxane, hanging drop, temperature 298K
|
Resolution 2.00 Å R-free 0.238 |
| 3U8X Crystal Structure of a chimera containing the N-terminal domain (residues 8-29) of drosophila Ciboulot and the C-terminal domain (residues 18-44) of bovine Thymosin-beta4, bound to G-actin-ATP Deposited 2011-10-17 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
3–377（375 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
hanging drop；pH 6.5；298 K；20% PEG3350, 0.2M MgAcetate pH6.5, 0.45M Guanidine HCl, 1% Dioxane, hanging drop, temperature 298K
|
Resolution 2.00 Å R-free 0.238 |
| 3U9Z Crystal structure between actin and a protein construct containing the first beta-thymosin domain of drosophila ciboulot (residues 2-58) with the three mutations N26D/Q27K/D28S Deposited 2011-10-20 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
hanging drop；pH 6.5；298 K；18% PEG3350, 0.05M NaAcetate pH4.7, 0.1M MgAcetate pH6.5, 0.32M Guanidine HCl, 0.8% Dioxane, hanging drop, temperature 298K
|
Resolution 2.09 Å R-free 0.227 |
| 3UE5 ECP-cleaved Actin in complex with Spir domain D Deposited 2011-10-28 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain A
3–377（375 aa）
|
Not recorded | CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 CL CHLORIDE ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；298 K；Tris pH 8.5, magnesium chloride, PEG-8000, vapor diffusion, hanging drop, temperature 298K
|
Resolution 2.76 Å R-free 0.240 |
| 3UE5 ECP-cleaved Actin in complex with Spir domain D Deposited 2011-10-28 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain A
3–377（375 aa）
|
Not recorded | CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 CL CHLORIDE ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；298 K；Tris pH 8.5, magnesium chloride, PEG-8000, vapor diffusion, hanging drop, temperature 298K
|
Resolution 2.76 Å R-free 0.240 |
| 3UE5 ECP-cleaved Actin in complex with Spir domain D Deposited 2011-10-28 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain A
3–377（375 aa）
|
Not recorded | CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 CL CHLORIDE ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；298 K；Tris pH 8.5, magnesium chloride, PEG-8000, vapor diffusion, hanging drop, temperature 298K
|
Resolution 2.76 Å R-free 0.240 |
| 4A7F Structure of the Actin-Tropomyosin-Myosin Complex (rigor ATM 3) Deposited 2011-11-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
Chain F
3–377（375 aa）
Chain I
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 CA CALCIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
5 MM HEPES-OH, 100 MM KCL, 2 MM MGCL2, 50 MM GLUTAMINE, 50 MM ARGININE；pH 7.2；5 MM HEPES-OH, 100 MM KCL, 2 MM MGCL2, 50 MM GLUTAMINE, 50 MM ARGININE
cryo-EM vitrification conditions
Cryogen ETHANE；LIQUID ETHANE
|
Resolution 7.70 Å |
| 4A7H Structure of the Actin-Tropomyosin-Myosin Complex (rigor ATM 2) Deposited 2011-11-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
Chain F
3–377（375 aa）
Chain G
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 CA CALCIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
5MM TRIS, 100MM KCL, 2MM MGCL2, 50MM GLUTAMINE, 50MM ARGININ；pH 7.2；5MM TRIS, 100MM KCL, 2MM MGCL2, 50MM GLUTAMINE, 50MM ARGININ
cryo-EM vitrification conditions
Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 90, TEMPERATURE- 101, INSTRUMENT- GATAN CRYOPLUNGE 3, METHOD- MANUAL BLOTTING FOR APPROXIMATELY 15 SECONDS,
|
Resolution 7.80 Å |
| 4A7L Structure of the Actin-Tropomyosin-Myosin Complex (rigor ATM 1) Deposited 2011-11-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
Chain F
3–377（375 aa）
Chain I
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 CA CALCIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
5MM TRIS, 100MM KCL, 2MM MGCL2, 50MM GLUTAMINE, 50MM ARGININ；pH 7.2；5MM TRIS, 100MM KCL, 2MM MGCL2, 50MM GLUTAMINE, 50MM ARGININ
cryo-EM vitrification conditions
Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 90, TEMPERATURE- 101, INSTRUMENT- GATAN CRYOPLUNGE 3, METHOD- MANUAL BLOTTING FOR APPROXIMATELY 15 SECONDS,
|
Resolution 8.10 Å |
| 4A7N Structure of bare F-actin filaments obtained from the same sample as the Actin-Tropomyosin-Myosin Complex Deposited 2011-11-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 CA CALCIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
5MM TRIS, 100MM KCL, 2MM MGCL2, 50MM GLUTAMINE, 50MM ARGININE；pH 7.2；5MM TRIS, 100MM KCL, 2MM MGCL2, 50MM GLUTAMINE, 50MM ARGININE
cryo-EM vitrification conditions
Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 90, TEMPERATURE- 101, INSTRUMENT- GATAN CRYOPLUNGE 3, METHOD- MANUAL BLOTTING FOR APPROXIMATELY 15 SECONDS,
|
Resolution 8.90 Å |
| 4B1V Structure of the Phactr1 RPEL-N domain bound to G-actin Deposited 2012-07-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
2–377（376 aa）
Fragment：RESIDUES 2-377
|
Not recorded | EDO 1,2-ETHANEDIOL × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 LAB LATRUNCULIN B × 1 MG MAGNESIUM ION × 1 GOL GLYCEROL × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.75 Å R-free 0.221 |
| 4B1V Structure of the Phactr1 RPEL-N domain bound to G-actin Deposited 2012-07-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
2–377（376 aa）
Fragment：RESIDUES 2-377
|
Not recorded | EDO 1,2-ETHANEDIOL × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 LAB LATRUNCULIN B × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.75 Å R-free 0.221 |
| 4B1W Structure of the Phactr1 RPEL-2 domain bound to actin Deposited 2012-07-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
2–377（376 aa）
|
Not recorded | LAB LATRUNCULIN B × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SO4 SULFATE ION × 1 GOL GLYCEROL × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.95 Å R-free 0.213 |
| 4B1X Structure of the Phactr1 RPEL-2 bound to G-actin Deposited 2012-07-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
2–377（376 aa）
|
Not recorded | LAB LATRUNCULIN B × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.80 Å R-free 0.213 |
| 4B1Y Structure of the Phactr1 RPEL-3 bound to G-actin Deposited 2012-07-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
2–377（376 aa）
|
Not recorded | LAB LATRUNCULIN B × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 1PE PENTAETHYLENE GLYCOL × 1 GOL GLYCEROL × 1 PEG DI(HYDROXYETHYL)ETHER × 3 P6G HEXAETHYLENE GLYCOL × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.29 Å R-free 0.174 |
| 4B1Z Structure of the Phactr1 RPEL domain bound to G-actin Deposited 2012-07-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
2–377（376 aa）
Chain B
2–377（376 aa）
Chain C
2–377（376 aa）
Chain D
2–377（376 aa）
Chain E
2–377（376 aa）
Chain F
2–377（376 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 GOL GLYCEROL × 8 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 3.30 Å R-free 0.236 |
| 4EAH Crystal structure of the formin homology 2 domain of FMNL3 bound to actin Deposited 2012-03-22 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain D
1–377（377 aa）
Chain G
1–377（377 aa）
|
Not recorded | ACT ACETATE ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；11% PEG 10000, 0.2 M magnesium acetate, 0.1 M MES, pH 6.5, Silver Bullet 33 (0.20% w/v D-(+)-Maltose monohydrate, 0.20% w/v D-(+)-Melibiose monohydrate, 0.20% w/v D-(+)-Raffinose pentahydrate, 0.20% w/v D-(+)-Trehalose dihydrate, 0.20% w/v Stachyose hydrate, 0.02 M HEPES sodium pH 6.8), Silver Bullet 70 (0.2% w/v Anthrone, 0.2% w/v Benzidine, 0.2% w/v N-(2-Acetamido)-2-aminoethanesulfonic acid, 0.2% w/v Phenylurea, 0.2% w/v -Alanine, 0.02 M HEPES sodium pH 6.8), VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.40 Å R-free 0.277 |
| 4EAH Crystal structure of the formin homology 2 domain of FMNL3 bound to actin Deposited 2012-03-22 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain F
1–377（377 aa）
Chain H
1–377（377 aa）
|
Not recorded | ACT ACETATE ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；11% PEG 10000, 0.2 M magnesium acetate, 0.1 M MES, pH 6.5, Silver Bullet 33 (0.20% w/v D-(+)-Maltose monohydrate, 0.20% w/v D-(+)-Melibiose monohydrate, 0.20% w/v D-(+)-Raffinose pentahydrate, 0.20% w/v D-(+)-Trehalose dihydrate, 0.20% w/v Stachyose hydrate, 0.02 M HEPES sodium pH 6.8), Silver Bullet 70 (0.2% w/v Anthrone, 0.2% w/v Benzidine, 0.2% w/v N-(2-Acetamido)-2-aminoethanesulfonic acid, 0.2% w/v Phenylurea, 0.2% w/v -Alanine, 0.02 M HEPES sodium pH 6.8), VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.40 Å R-free 0.277 |
| 4GY2 Crystal structure of apo-Ia-actin complex Deposited 2012-09-05 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
3–377（375 aa）
|
Not recorded | PO4 PHOSPHATE ION × 1 CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 LAR LATRUNCULIN A × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.13 K；18% PEG 1500, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.13K
|
Resolution 2.71 Å R-free 0.257 |
| 4H03 Crystal structure of NAD+-Ia-actin complex Deposited 2012-09-07 | Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 PO4 PHOSPHATE ION × 1 EDO 1,2-ETHANEDIOL × 48 CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 LAR LATRUNCULIN A × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.13 K；18% PEG1500, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.13K
|
Resolution 1.75 Å R-free 0.233 |
| 4H0T Crystal structure of Ia-ADPR-actin complex Deposited 2012-09-10 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
3–377（375 aa）
|
Not recorded | PO4 PHOSPHATE ION × 1 EDO 1,2-ETHANEDIOL × 18 AR6 [(2R,3S,4R,5R)-5-(6-AMINOPURIN-9-YL)-3,4-DIHYDROXY-OXOLAN-2-YL]METHYL[HYDROXY-[[(2R,3S,4R,5S)-3,4,5-TRIHYDROXYOXOLAN-2-YL]METHOXY]PHOSPHORYL] HYDROGEN PHOSPHATE × 1 CA CALCIUM ION × 1 LAR LATRUNCULIN A × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.13 K；18% PEG1500, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.13K
|
Resolution 2.20 Å R-free 0.240 |
| 4H0V Crystal structure of NAD+-Ia(E378S)-actin complex Deposited 2012-09-10 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
3–377（375 aa）
|
Not recorded | NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 PO4 PHOSPHATE ION × 1 EDO 1,2-ETHANEDIOL × 39 CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 LAR LATRUNCULIN A × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.13 K；18% PEG1500, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.13K
|
Resolution 2.03 Å R-free 0.234 |
| 4H0X Crystal structure of NAD+-Ia(E380A)-actin complex Deposited 2012-09-10 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
3–377（375 aa）
|
Not recorded | NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 PO4 PHOSPHATE ION × 1 EDO 1,2-ETHANEDIOL × 22 CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 LAR LATRUNCULIN A × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.13 K；18% PEG1500, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.13K
|
Resolution 2.33 Å R-free 0.251 |
| 4H0Y Crystal structure of NAD+-Ia(E380S)-actin complex Deposited 2012-09-10 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
3–377（375 aa）
|
Not recorded | NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 PO4 PHOSPHATE ION × 1 EDO 1,2-ETHANEDIOL × 52 CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 LAR LATRUNCULIN A × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.13 K；18% PEG1500, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.13K
|
Resolution 1.94 Å R-free 0.233 |
| 4K41 Crystal structure of actin in complex with marine macrolide kabiramide C Deposited 2013-04-11 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–377（375 aa）
|
Not recorded | CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 KAB KABIRAMIDE C × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；298 K；0.1 M MES pH 5.5, 0.1 M CaCl2, 12% 1,6-Hexanediol and 17% Polyethylene glycol 1500, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.40 Å R-free 0.195 |
| 4K42 Crystal structure of actin in complex with synthetic AplC tail analogue SF01 [(3R,4S,5R,6S,10R,11R,12R)-11-(acetyloxy)-1-(benzyloxy)-14-[formyl(methyl)amino]-5-hydroxy-4,6,10,12-tetramethyl-9-oxotetradecan-3-yl propanoate] Deposited 2013-04-11 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–377（375 aa）
|
Not recorded | CA CALCIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NWM (3R,4S,5R,6S,10R,11R,12R)-11-(acetyloxy)-1-(benzyloxy)-14-[formyl(methyl)amino]-5-hydroxy-4,6,10,12-tetramethyl-9-oxotetradecan-3-yl propanoate × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；298 K；0.1 M BIS-TRIS pH 5.5, 25% Polyethylene glycol 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.90 Å R-free 0.271 |
| 4K42 Crystal structure of actin in complex with synthetic AplC tail analogue SF01 [(3R,4S,5R,6S,10R,11R,12R)-11-(acetyloxy)-1-(benzyloxy)-14-[formyl(methyl)amino]-5-hydroxy-4,6,10,12-tetramethyl-9-oxotetradecan-3-yl propanoate] Deposited 2013-04-11 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
3–377（375 aa）
|
Not recorded | CA CALCIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NWM (3R,4S,5R,6S,10R,11R,12R)-11-(acetyloxy)-1-(benzyloxy)-14-[formyl(methyl)amino]-5-hydroxy-4,6,10,12-tetramethyl-9-oxotetradecan-3-yl propanoate × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；298 K；0.1 M BIS-TRIS pH 5.5, 25% Polyethylene glycol 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.90 Å R-free 0.271 |
| 4K42 Crystal structure of actin in complex with synthetic AplC tail analogue SF01 [(3R,4S,5R,6S,10R,11R,12R)-11-(acetyloxy)-1-(benzyloxy)-14-[formyl(methyl)amino]-5-hydroxy-4,6,10,12-tetramethyl-9-oxotetradecan-3-yl propanoate] Deposited 2013-04-11 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
3–377（375 aa）
|
Not recorded | CA CALCIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NWM (3R,4S,5R,6S,10R,11R,12R)-11-(acetyloxy)-1-(benzyloxy)-14-[formyl(methyl)amino]-5-hydroxy-4,6,10,12-tetramethyl-9-oxotetradecan-3-yl propanoate × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；298 K；0.1 M BIS-TRIS pH 5.5, 25% Polyethylene glycol 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.90 Å R-free 0.271 |
| 4K42 Crystal structure of actin in complex with synthetic AplC tail analogue SF01 [(3R,4S,5R,6S,10R,11R,12R)-11-(acetyloxy)-1-(benzyloxy)-14-[formyl(methyl)amino]-5-hydroxy-4,6,10,12-tetramethyl-9-oxotetradecan-3-yl propanoate] Deposited 2013-04-11 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
3–377（375 aa）
|
Not recorded | CA CALCIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NWM (3R,4S,5R,6S,10R,11R,12R)-11-(acetyloxy)-1-(benzyloxy)-14-[formyl(methyl)amino]-5-hydroxy-4,6,10,12-tetramethyl-9-oxotetradecan-3-yl propanoate × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；298 K；0.1 M BIS-TRIS pH 5.5, 25% Polyethylene glycol 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.90 Å R-free 0.271 |
| 4K43 Crystal structure of actin in complex with synthetic AplC tail analogue GC04 [N-{(1E,4R,5R,7E,9S,10S,11S)-4,10-dimethoxy-11-[(2S,4S,5S)-2-(4-methoxyphenyl)-5-methyl-1,3-dioxan-4-yl]-5,9-dimethyl-6-oxododeca-1,7-dien-1-yl}-N-methylformamide] Deposited 2013-04-11 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–377（375 aa）
|
Not recorded | CA CALCIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 1PO N-{(1E,4R,5R,7E,9S,10S,11S)-4,10-dimethoxy-11-[(2S,4S,5S)-2-(4-methoxyphenyl)-5-methyl-1,3-dioxan-4-yl]-5,9-dimethyl-6-oxododeca-1,7-dien-1-yl}-N-methylformamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；298 K；0.1 M BIS-TRIS pH 5.5, 25% Polyethylene glycol 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.90 Å R-free 0.277 |
| 4K43 Crystal structure of actin in complex with synthetic AplC tail analogue GC04 [N-{(1E,4R,5R,7E,9S,10S,11S)-4,10-dimethoxy-11-[(2S,4S,5S)-2-(4-methoxyphenyl)-5-methyl-1,3-dioxan-4-yl]-5,9-dimethyl-6-oxododeca-1,7-dien-1-yl}-N-methylformamide] Deposited 2013-04-11 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
3–377（375 aa）
|
Not recorded | CA CALCIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 1PO N-{(1E,4R,5R,7E,9S,10S,11S)-4,10-dimethoxy-11-[(2S,4S,5S)-2-(4-methoxyphenyl)-5-methyl-1,3-dioxan-4-yl]-5,9-dimethyl-6-oxododeca-1,7-dien-1-yl}-N-methylformamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；298 K；0.1 M BIS-TRIS pH 5.5, 25% Polyethylene glycol 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.90 Å R-free 0.277 |
| 4PKG Complex of ATP-actin With the N-terminal Actin-Binding Domain of Tropomodulin Deposited 2014-05-14 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；0.18 M sodium fluoride, 11% w/v PEG3350, 1% v/v PEG1000, 1% v/v PEG400
|
Resolution 1.80 Å R-free 0.187 |
| 4PKH Complex of ADP-actin With the N-terminal Actin-Binding Domain of Tropomodulin Deposited 2014-05-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–377（377 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 1 CA CALCIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；0.18 M sodium fluoride, 11% w/v PEG3350, 1% v/v PEG1000, 1% v/v PEG400
|
Resolution 2.15 Å R-free 0.326 |
| 4PKH Complex of ADP-actin With the N-terminal Actin-Binding Domain of Tropomodulin Deposited 2014-05-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric |
Chain D
1–377（377 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 1 CA CALCIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；0.18 M sodium fluoride, 11% w/v PEG3350, 1% v/v PEG1000, 1% v/v PEG400
|
Resolution 2.15 Å R-free 0.326 |
| 4PKH Complex of ADP-actin With the N-terminal Actin-Binding Domain of Tropomodulin Deposited 2014-05-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 3 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric |
Chain F
1–377（377 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 1 CA CALCIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；0.18 M sodium fluoride, 11% w/v PEG3350, 1% v/v PEG1000, 1% v/v PEG400
|
Resolution 2.15 Å R-free 0.326 |
| 4PKH Complex of ADP-actin With the N-terminal Actin-Binding Domain of Tropomodulin Deposited 2014-05-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 4 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric |
Chain I
1–377（377 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 1 CA CALCIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；0.18 M sodium fluoride, 11% w/v PEG3350, 1% v/v PEG1000, 1% v/v PEG400
|
Resolution 2.15 Å R-free 0.326 |
| 4PKI Complex of ATP-actin With the C-terminal Actin-Binding Domain of Tropomodulin Deposited 2014-05-14 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；0.25 M sodium chloride, 12% w/v PEG3350
|
Resolution 2.30 Å R-free 0.201 |
| 4PL8 Structure of rabbit skeletal muscle actin in complex with a hybrid peptide comprising thymosin beta4 and the lysine-rich region of Cordon-Bleu Deposited 2014-05-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ATP ADENOSINE-5'-TRIPHOSPHATE × 2 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 3.5；288 K；0.1 M citric acid, 15% (w/v) PEG 3350
|
Resolution 2.00 Å R-free 0.207 |
| 4V0U The crystal structure of ternary PP1G-PPP1R15B and G-actin complex Deposited 2014-09-18 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 10 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain M
3–377（375 aa）
Fragment：RESIDUES 3-377
|
Not recorded | LAB LATRUNCULIN B × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7；0.2M CACL2, 0.1 M HEPES, PH7.0, 20% PEG6000
|
Resolution 7.88 Å R-free 0.400 |
| 4V0U The crystal structure of ternary PP1G-PPP1R15B and G-actin complex Deposited 2014-09-18 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 6 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–377（375 aa）
Fragment：RESIDUES 3-377
|
Not recorded | LAB LATRUNCULIN B × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7；0.2M CACL2, 0.1 M HEPES, PH7.0, 20% PEG6000
|
Resolution 7.88 Å R-free 0.400 |
| 4V0U The crystal structure of ternary PP1G-PPP1R15B and G-actin complex Deposited 2014-09-18 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 7 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
3–377（375 aa）
Fragment：RESIDUES 3-377
|
Not recorded | LAB LATRUNCULIN B × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7；0.2M CACL2, 0.1 M HEPES, PH7.0, 20% PEG6000
|
Resolution 7.88 Å R-free 0.400 |
| 4V0U The crystal structure of ternary PP1G-PPP1R15B and G-actin complex Deposited 2014-09-18 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 8 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
3–377（375 aa）
Fragment：RESIDUES 3-377
|
Not recorded | LAB LATRUNCULIN B × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7；0.2M CACL2, 0.1 M HEPES, PH7.0, 20% PEG6000
|
Resolution 7.88 Å R-free 0.400 |
| 4V0U The crystal structure of ternary PP1G-PPP1R15B and G-actin complex Deposited 2014-09-18 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 9 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain L
3–377（375 aa）
Fragment：RESIDUES 3-377
|
Not recorded | LAB LATRUNCULIN B × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7；0.2M CACL2, 0.1 M HEPES, PH7.0, 20% PEG6000
|
Resolution 7.88 Å R-free 0.400 |
| 4WYB Structure of the Bud6 flank domain in complex with actin Deposited 2014-11-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–377（377 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；3.5M sodium formate, 0.1M CaCl2, and 5 mM TCEP
|
Resolution 3.49 Å R-free 0.258 |
| 4WYB Structure of the Bud6 flank domain in complex with actin Deposited 2014-11-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 10 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain S
1–377（377 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；3.5M sodium formate, 0.1M CaCl2, and 5 mM TCEP
|
Resolution 3.49 Å R-free 0.258 |
| 4WYB Structure of the Bud6 flank domain in complex with actin Deposited 2014-11-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 11 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain U
1–377（377 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；3.5M sodium formate, 0.1M CaCl2, and 5 mM TCEP
|
Resolution 3.49 Å R-free 0.258 |
| 4WYB Structure of the Bud6 flank domain in complex with actin Deposited 2014-11-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 12 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain X
1–377（377 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；3.5M sodium formate, 0.1M CaCl2, and 5 mM TCEP
|
Resolution 3.49 Å R-free 0.258 |
| 4WYB Structure of the Bud6 flank domain in complex with actin Deposited 2014-11-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
1–377（377 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；3.5M sodium formate, 0.1M CaCl2, and 5 mM TCEP
|
Resolution 3.49 Å R-free 0.258 |
| 4WYB Structure of the Bud6 flank domain in complex with actin Deposited 2014-11-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain E
1–377（377 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；3.5M sodium formate, 0.1M CaCl2, and 5 mM TCEP
|
Resolution 3.49 Å R-free 0.258 |
| 4WYB Structure of the Bud6 flank domain in complex with actin Deposited 2014-11-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain G
1–377（377 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；3.5M sodium formate, 0.1M CaCl2, and 5 mM TCEP
|
Resolution 3.49 Å R-free 0.258 |
| 4WYB Structure of the Bud6 flank domain in complex with actin Deposited 2014-11-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 5 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain I
1–377（377 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；3.5M sodium formate, 0.1M CaCl2, and 5 mM TCEP
|
Resolution 3.49 Å R-free 0.258 |
| 4WYB Structure of the Bud6 flank domain in complex with actin Deposited 2014-11-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 6 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain K
1–377（377 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；3.5M sodium formate, 0.1M CaCl2, and 5 mM TCEP
|
Resolution 3.49 Å R-free 0.258 |
| 4WYB Structure of the Bud6 flank domain in complex with actin Deposited 2014-11-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 7 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain M
1–377（377 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；3.5M sodium formate, 0.1M CaCl2, and 5 mM TCEP
|
Resolution 3.49 Å R-free 0.258 |
| 4WYB Structure of the Bud6 flank domain in complex with actin Deposited 2014-11-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 8 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain O
1–377（377 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；3.5M sodium formate, 0.1M CaCl2, and 5 mM TCEP
|
Resolution 3.49 Å R-free 0.258 |
| 4WYB Structure of the Bud6 flank domain in complex with actin Deposited 2014-11-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 9 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain Q
1–377（377 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；3.5M sodium formate, 0.1M CaCl2, and 5 mM TCEP
|
Resolution 3.49 Å R-free 0.258 |
| 4Z94 Actin Complex With a Chimera of Tropomodulin-1 and Leiomodin-1 Actin-Binding Site 2 Deposited 2015-04-09 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.8；291.5 K；20% PEG3350, 200 mM lithium sulfate, 100 mM Tris, pH 8.8, 15% glycerol
|
Resolution 2.40 Å R-free 0.243 |
| 5H53 The structure of rabbit skeletal muscle actomyosin rigor complex at 5.2 angstrom. Deposited 2016-11-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric |
Chain D
3–377（375 aa）
Fragment：UNP residues 3-377
Chain E
3–377（375 aa）
Fragment：UNP residues 3-377
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.20 Å |
| 5JLF Structure of the F-actin-tropomyosin complex (Reprocessed) Deposited 2016-04-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；5 mM Tris-HCl pH 7.5, 1 mM DTT, 100 mM KCl, and 2 mM MgCl2
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was applied to a glow-discharged holey carbon grid, incubated for 10 s and manually blotted for 3 s from the backside with filter paper.
|
Resolution 3.60 Å |
| 5KG8 Rigor myosin X co-complexed with an actin filament Deposited 2016-06-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 9.10 Å |
| 5MVA Structure of the thin filament at high calcium concentration Deposited 2017-01-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 23 PDB declaration: 23-meric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
Chain F
3–377（375 aa）
Chain G
3–377（375 aa）
Chain H
3–377（375 aa）
Chain I
3–377（375 aa）
Chain J
3–377（375 aa）
Chain K
3–377（375 aa）
Chain L
3–377（375 aa）
Chain M
3–377（375 aa）
Chain N
3–377（375 aa）
Chain O
3–377（375 aa）
Chain P
3–377（375 aa）
Chain Q
3–377（375 aa）
Chain R
3–377（375 aa）
Chain S
3–377（375 aa）
Chain T
3–377（375 aa）
Chain U
3–377（375 aa）
Chain V
3–377（375 aa）
Chain W
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 23 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
|
Resolution 27.70 Å |
| 5MVY Thin Filament at low calcium concentration Deposited 2017-01-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 23 PDB declaration: 23-meric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
Chain F
3–377（375 aa）
Chain G
3–377（375 aa）
Chain H
3–377（375 aa）
Chain I
3–377（375 aa）
Chain J
3–377（375 aa）
Chain K
3–377（375 aa）
Chain L
3–377（375 aa）
Chain M
3–377（375 aa）
Chain N
3–377（375 aa）
Chain O
3–377（375 aa）
Chain P
3–377（375 aa）
Chain Q
3–377（375 aa）
Chain R
3–377（375 aa）
Chain S
3–377（375 aa）
Chain T
3–377（375 aa）
Chain U
3–377（375 aa）
Chain V
3–377（375 aa）
Chain W
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 23 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
|
Resolution 28.40 Å |
| 5ONV Cryo-EM structure of F-actin in complex with ADP Deposited 2017-08-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；5 mM HEPES pH 7.5, 0.1 M KCl, 2 mM MgCl2, 2 mM, 2 mM NaN3, 1 mM TCEP and 0.2 mM ADP.
cryo-EM vitrification conditions
Cryogen ETHANE；Manual backside blotting using Whatman filter paper No.5.
|
Resolution 4.10 Å |
| 5OOC Cryo-EM structure of jasplakinolide-stabilized F-actin in complex with ADP Deposited 2017-08-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 9ZK (4~{R},7~{R},10~{S},13~{S},15~{E},19~{S})-10-(4-azanylbutyl)-4-(4-hydroxyphenyl)-7-(1~{H}-indol-3-ylmethyl)-8,13,15,19-tetramethyl-1-oxa-5,8,11-triazacyclononadec-15-ene-2,6,9,12-tetrone × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；5 mM Tris pH 7.5, 2 mM NaN3, 1 mM DTT, 100 mM KCl and 2 mM MgCl2, 0.12 %(v/v) DMSO
cryo-EM vitrification conditions
Cryogen ETHANE；Manual backside blotting using Whatman filter paper No.5.
|
Resolution 3.60 Å |
| 5OOD Cryo-EM structure of jasplakinolide-stabilized F-actin in complex with ADP-Pi Deposited 2017-08-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 PO4 PHOSPHATE ION × 5 9ZK (4~{R},7~{R},10~{S},13~{S},15~{E},19~{S})-10-(4-azanylbutyl)-4-(4-hydroxyphenyl)-7-(1~{H}-indol-3-ylmethyl)-8,13,15,19-tetramethyl-1-oxa-5,8,11-triazacyclononadec-15-ene-2,6,9,12-tetrone × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；5 mM Tris pH 7.5, 2 mM NaN3, 1 mM DTT, 100 mM KCl and 2 mM MgCl2, 9.0 %(v/v) DMSO
cryo-EM vitrification conditions
Cryogen ETHANE；Manual backside blotting using Whatman filter paper No.5.
|
Resolution 3.70 Å |
| 5OOE Cryo-EM structure of F-actin in complex with AppNHp (AMPPNP) Deposited 2017-08-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；5 mM HEPES pH 7.5, 0.1 M KCl, 2 mM MgCl2, 2 mM, 2 mM NaN3, 0.5 mM TCEP and 0.4 mM AppNHp.
cryo-EM vitrification conditions
Cryogen ETHANE；Manual backside blotting using Whatman filter paper No.5.
|
Resolution 3.60 Å |
| 5OOF Cryo-EM structure of F-actin in complex with ADP-BeFx Deposited 2017-08-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；5 mM HEPES pH 7.5, 0.1 M KCl, 2 mM MgCl2, 2 mM, 2 mM NaN3, 1 mM TCEP, 0.2 mM ADP, 0.2 mM BeF2 and 5 mM NaF.
cryo-EM vitrification conditions
Cryogen ETHANE；Manual backside blotting using Whatman filter paper No.5.
|
Resolution 3.40 Å |
| 5UBO Mical-oxidized Actin complex with Gelsolin Segment 1 Deposited 2016-12-21 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–377（377 aa）
Fragment：unp residues 1-377
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.6；298 K；PEG 6000, NaCl, imidazole, ATP, calcium
|
Resolution 2.39 Å R-free 0.191 |
| 5YEE Crystal structure of LokiProfilin1/Rabbit Actin Complex Deposited 2017-09-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
1–377（377 aa）
|
Not recorded | LAB LATRUNCULIN B × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；100 mM Hepes, pH 7.0, 20 % w/v Polyethylene glycol 6,000, 200 mM NaCl, 10 mM ATP disodium salt
|
Resolution 1.81 Å R-free 0.249 |
| 5YPU Crystal structure of an actin monomer in complex with the nucleator Cordon-Bleu MET72NLE WH2-motif peptide Deposited 2017-11-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
7–374（368 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.9；298.15 K；20mM MES pH 4.9, 0.2mM CaCl2,2H2O, 20%(w/v) PEG 3,350
|
Resolution 2.00 Å R-free 0.240 |
| 5YPU Crystal structure of an actin monomer in complex with the nucleator Cordon-Bleu MET72NLE WH2-motif peptide Deposited 2017-11-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
7–374（368 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.9；298.15 K；20mM MES pH 4.9, 0.2mM CaCl2,2H2O, 20%(w/v) PEG 3,350
|
Resolution 2.00 Å R-free 0.240 |
| 5ZZA OdinProfilin/Rabbit Actin Complex Deposited 2018-05-31 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
5–377（373 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | LAB LATRUNCULIN B × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5；291 K；100 mM Citrate, 20% PEG6000
|
Resolution 1.53 Å R-free 0.179 |
| 5ZZB LokiProfilin2/Rabbit Actin Complex Deposited 2018-05-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
7–377（371 aa）
|
Not recorded | LAB LATRUNCULIN B × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；100 mM PCTP
25% PEG1500
|
Resolution 2.30 Å R-free 0.274 |
| 5ZZB LokiProfilin2/Rabbit Actin Complex Deposited 2018-05-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain D
7–377（371 aa）
|
Not recorded | LAB LATRUNCULIN B × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；100 mM PCTP
25% PEG1500
|
Resolution 2.30 Å R-free 0.274 |
| 6AV9 CryoEM structure of Mical Oxidized Actin (Class 1) Deposited 2017-09-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blot Force 1, Blot time 4s
|
Resolution 3.90 Å |
| 6AVB CryoEM structure of Mical Oxidized Actin (Class 1) Deposited 2017-09-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blot Force 1, Blot time 4s
|
Resolution 3.90 Å |
| 6BIH The Structure of the Actin-Smooth Muscle Myosin Motor Domain Complex in the Rigor State Deposited 2017-11-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric |
Chain C
1–377（377 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 7 MG MAGNESIUM ION × 7 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；actin buffer: 10 mM imidazole, 10 mM KCl, 1.0 mM MgCl2, 1.0 mM EGTA, 0.5 mM DTT, pH 7.4, myosin buffer: 10 mM imidazole, 10 mM KCl, 1.0 mM MgCl2, 1.0 mM EGTA, 0.5 mM DTT, pH 7.0
cryo-EM vitrification conditions
Cryogen ETHANE；Some specimens were frozen manually using a homemade plunger.
|
Resolution 6.00 Å |
| 6BIH The Structure of the Actin-Smooth Muscle Myosin Motor Domain Complex in the Rigor State Deposited 2017-11-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
1–377（377 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；actin buffer: 10 mM imidazole, 10 mM KCl, 1.0 mM MgCl2, 1.0 mM EGTA, 0.5 mM DTT, pH 7.4, myosin buffer: 10 mM imidazole, 10 mM KCl, 1.0 mM MgCl2, 1.0 mM EGTA, 0.5 mM DTT, pH 7.0
cryo-EM vitrification conditions
Cryogen ETHANE；Some specimens were frozen manually using a homemade plunger.
|
Resolution 6.00 Å |
| 6BNO Structure of bare actin filament Deposited 2017-11-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric |
Chain A
1–373（373 aa）
Chain B
1–373（373 aa）
Chain C
1–373（373 aa）
Chain D
1–373（373 aa）
Chain E
1–373（373 aa）
Chain F
1–373（373 aa）
Chain G
1–373（373 aa）
Chain H
1–373（373 aa）
|
Not recorded | MG MAGNESIUM ION × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；Buffer was filtered through 0.44 um filter and degassed.
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was applied to a glow-discharged holey carbon grid, incubated for 60 seconds and blotted for 3 seconds from the backside with filter paper.
|
Resolution 5.50 Å |
| 6BNP CryoEM structure of MyosinVI-actin complex in the rigor (nucleotide-free) state Deposited 2017-11-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric |
Chain A
1–373（373 aa）
Chain B
1–373（373 aa）
Chain C
1–373（373 aa）
Chain D
1–373（373 aa）
Chain E
1–373（373 aa）
Chain F
1–373（373 aa）
Chain G
1–373（373 aa）
Chain H
1–373（373 aa）
|
Not recorded | MG MAGNESIUM ION × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；Buffer was filtered through 0.44 um filter and degassed.
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was applied to a glow-discharged holey carbon grid. 3 uL actin was incubated for 60 seconds. 3 uL of myosin VI was added and incubated for 60 seconds. 3 uL solution was removed. An additional 3 uL of myosin VI was applied. After 60 seconds, 3 uL solution was removed, and the grid was blotted for 3 seconds from the backside with filter paper.
|
Resolution 4.60 Å |
| 6BNQ CryoEM structure of Myosin VI-Actin complex in the ADP state Deposited 2017-11-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric |
Chain A
1–373（373 aa）
Chain B
1–373（373 aa）
Chain C
1–373（373 aa）
Chain D
1–373（373 aa）
Chain E
1–373（373 aa）
Chain F
1–373（373 aa）
Chain G
1–373（373 aa）
Chain H
1–373（373 aa）
|
Not recorded | MG MAGNESIUM ION × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；Buffer was filtered through 0.44 um filter and degassed.
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was applied to a glow-discharged holey carbon grid. 3 uL actin was incubated for 60 seconds. 3 uL of myosin VI was added and incubated for 60 seconds. 3 uL solution was removed. An additional 3 uL of myosin VI was applied. After 60 seconds, 3 uL solution was removed, and the grid was blotted for 3 seconds from the backside with filter paper.
|
Resolution 5.50 Å |
| 6BNU Structure of bare actin filament, backbone-averaged with sidechains truncated to alanine Deposited 2017-11-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric |
Chain A
1–373（373 aa）
Chain B
1–373（373 aa）
Chain C
1–373（373 aa）
Chain D
1–373（373 aa）
Chain E
1–373（373 aa）
Chain F
1–373（373 aa）
Chain G
1–373（373 aa）
Chain H
1–373（373 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；Buffer was filtered through 0.44 um filter and degassed.
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was applied to a glow-discharged holey carbon grid, incubated for 60 seconds, and blotted for 3 seconds from the backside with filter paper.
|
Resolution 7.50 Å |
| 6BNV CryoEM structure of MyosinVI-actin complex in the rigor (nucleotide-free) state, backbone-averaged with side chains truncated to alanine Deposited 2017-11-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric |
Chain A
1–373（373 aa）
Chain B
1–373（373 aa）
Chain C
1–373（373 aa）
Chain D
1–373（373 aa）
Chain E
1–373（373 aa）
Chain F
1–373（373 aa）
Chain G
1–373（373 aa）
Chain H
1–373（373 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；Buffer was filtered through 0.44 um filter and degassed.
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was applied to a glow-discharged holey carbon grid. 3 uL actin was incubated for 60 seconds. 3 uL of myosin VI was added and incubated for 60 seconds. 3 uL solution was removed. An additional 3 uL of myosin VI was applied. After 60 seconds, 3 uL solution was removed, and the grid was blotted for 3 seconds from the backside with filter paper.
|
Resolution 4.60 Å |
| 6BNW CryoEM structure of Myosin VI-Actin complex in the ADP state, backbone-averaged with side chains truncated to alanine Deposited 2017-11-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric |
Chain A
1–373（373 aa）
Chain B
1–373（373 aa）
Chain C
1–373（373 aa）
Chain D
1–373（373 aa）
Chain E
1–373（373 aa）
Chain F
1–373（373 aa）
Chain G
1–373（373 aa）
Chain H
1–373（373 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；Buffer was filtered through 0.44 um filter and degassed
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was applied to a glow-discharged holey carbon grid. 3 uL actin was incubated for 60 seconds. 3 uL of myosin VI was added and incubated for 60 seconds. 3 uL solution was removed. An additional 3 uL of myosin VI was applied. After 60 seconds, 3 uL solution was removed, and the grid was blotted for 3 seconds from the backside with filter paper.
|
Resolution 5.50 Å |
| 6C1D High-Resolution Cryo-EM Structures of Actin-bound Myosin States Reveal the Mechanism of Myosin Force Sensing Deposited 2018-01-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: heptameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 6 MG MAGNESIUM ION × 6 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 6C1G High-Resolution Cryo-EM Structures of Actin-bound Myosin States Reveal the Mechanism of Myosin Force Sensing Deposited 2018-01-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Not recorded | MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 6 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 6C1H High-Resolution Cryo-EM Structures of Actin-bound Myosin States Reveal the Mechanism of Myosin Force Sensing Deposited 2018-01-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 6FHL Cryo-EM structure of F-actin in complex with ADP-Pi Deposited 2018-01-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 PO4 PHOSPHATE ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；5 mM HEPES pH 7.5, 0.05 M KCl, 2 mM MgCl2, 2 mM NaN3, 0.5 mM TCEP, 0.2 mM ADP, 50 mM potassium phosphate.
cryo-EM vitrification conditions
Cryogen ETHANE；8s blotting, 1s drain time, -25 force
|
Resolution 3.30 Å |
| 6FM2 CARP domain of mouse cyclase-associated protein 1 (CAP1) bound to ADP-actin Deposited 2018-01-30 | Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；0.1 M Tris-HCl, 0.2 M LiCl, 20% (w/v) PEG8000
|
Resolution 2.80 Å R-free 0.234 |
| 6GVC Structure of ArhGAP12 bound to G-Actin Deposited 2018-06-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
1–377（377 aa）
|
Not recorded | EDO 1,2-ETHANEDIOL × 2 LAB LATRUNCULIN B × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；20% PEG 3350,
0.2M Sodium Thiocyanate
0.1 M Bis Tris Propane pH6.5
|
Resolution 2.60 Å R-free 0.251 |
| 6GVC Structure of ArhGAP12 bound to G-Actin Deposited 2018-06-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–377（377 aa）
|
Not recorded | EDO 1,2-ETHANEDIOL × 3 LAB LATRUNCULIN B × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；20% PEG 3350,
0.2M Sodium Thiocyanate
0.1 M Bis Tris Propane pH6.5
|
Resolution 2.60 Å R-free 0.251 |
| 6GVC Structure of ArhGAP12 bound to G-Actin Deposited 2018-06-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
1–377（377 aa）
|
Not recorded | EDO 1,2-ETHANEDIOL × 1 LAB LATRUNCULIN B × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；20% PEG 3350,
0.2M Sodium Thiocyanate
0.1 M Bis Tris Propane pH6.5
|
Resolution 2.60 Å R-free 0.251 |
| 6GVC Structure of ArhGAP12 bound to G-Actin Deposited 2018-06-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain D
1–377（377 aa）
|
Not recorded | EDO 1,2-ETHANEDIOL × 2 LAB LATRUNCULIN B × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；20% PEG 3350,
0.2M Sodium Thiocyanate
0.1 M Bis Tris Propane pH6.5
|
Resolution 2.60 Å R-free 0.251 |
| 6JBK Crystal structure of an actin monomer in complex with the nucleator Cordon-Bleu WH2-motif peptide mutant. T22V Deposited 2019-01-25 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；298.15 K；20mM MES, pH 4.5, 0.2mM CaCl2.2H2O, 11% PEG 3350
|
Resolution 2.45 Å R-free 0.239 |
| 6JBK Crystal structure of an actin monomer in complex with the nucleator Cordon-Bleu WH2-motif peptide mutant. T22V Deposited 2019-01-25 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；298.15 K；20mM MES, pH 4.5, 0.2mM CaCl2.2H2O, 11% PEG 3350
|
Resolution 2.45 Å R-free 0.239 |
| 6JBK Crystal structure of an actin monomer in complex with the nucleator Cordon-Bleu WH2-motif peptide mutant. T22V Deposited 2019-01-25 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain E
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；298.15 K；20mM MES, pH 4.5, 0.2mM CaCl2.2H2O, 11% PEG 3350
|
Resolution 2.45 Å R-free 0.239 |
| 6JBK Crystal structure of an actin monomer in complex with the nucleator Cordon-Bleu WH2-motif peptide mutant. T22V Deposited 2019-01-25 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain G
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；298.15 K；20mM MES, pH 4.5, 0.2mM CaCl2.2H2O, 11% PEG 3350
|
Resolution 2.45 Å R-free 0.239 |
| 6JCU Crystal structure of an actin monomer in complex with a nucleator Cordon-Bleu WH2-motif peptide mutant. T22V, H11R Deposited 2019-01-30 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298.15 K；0.2M Ammonium nitrate, 20% PEG 3350
|
Resolution 2.30 Å R-free 0.230 |
| 6JCU Crystal structure of an actin monomer in complex with a nucleator Cordon-Bleu WH2-motif peptide mutant. T22V, H11R Deposited 2019-01-30 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298.15 K；0.2M Ammonium nitrate, 20% PEG 3350
|
Resolution 2.30 Å R-free 0.230 |
| 6JH8 Crystal structure of an actin monomer in complex with a chimeric peptide of Cordon-Bleu WH2 mutant and MIM. Deposited 2019-02-17 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；298.15 K；0.1M sodium acetate trihydrate, pH 4.6, 10% PEG 4000
|
Resolution 2.15 Å R-free 0.229 |
| 6JH9 Crystal structure of an actin monomer in complex with a chimeric peptide of Cordon-Bleu WH2 mutant and MIM. Lys18Arg Deposited 2019-02-17 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 2 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.8；298.15 K；0.1M sodium acetate trihydrate, pH 4.8, 8% PEG 4000
|
Resolution 1.74 Å R-free 0.206 |
| 6KN7 Structure of human cardiac thin filament in the calcium free state Deposited 2019-08-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 29 PDB declaration: 29-meric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
Chain F
3–377（375 aa）
Chain G
3–377（375 aa）
Chain H
3–377（375 aa）
Chain I
3–377（375 aa）
Chain J
3–377（375 aa）
Chain K
3–377（375 aa）
Chain L
3–377（375 aa）
Chain M
3–377（375 aa）
Chain N
3–377（375 aa）
Chain O
3–377（375 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 15 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.60 Å |
| 6KN8 Structure of human cardiac thin filament in the calcium bound state Deposited 2019-08-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 29 PDB declaration: 29-meric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
Chain F
3–377（375 aa）
Chain G
3–377（375 aa）
Chain H
3–377（375 aa）
Chain I
3–377（375 aa）
Chain J
3–377（375 aa）
Chain K
3–377（375 aa）
Chain L
3–377（375 aa）
Chain M
3–377（375 aa）
Chain N
3–377（375 aa）
Chain O
3–377（375 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 15 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.80 Å |
| 6MGO Structure of rabbit actin in complex with Mycalolide B Deposited 2018-09-14 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–377（377 aa）
|
Not recorded | JQV Mycalolide B × 1 CA CALCIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；PEG 8000, magnesium acetate, cacodylate
|
Resolution 2.20 Å R-free 0.195 |
| 6NAS Ternary Complex of Ac-Alpha-Actin with Profilin and AcCoA-NAA80 Deposited 2018-12-06 | Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 LAB LATRUNCULIN B × 1 GOL GLYCEROL × 3 ACO ACETYL COENZYME *A × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；290 K；16% Peg3350, 0.1 MES pH 6.5, 0.2M NH4NO3
|
Resolution 2.90 Å R-free 0.239 |
| 6NBE Ternary Complex of Ac-Alpha-Actin with Profilin and CoA-NAA80 Deposited 2018-12-07 | Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 LAB LATRUNCULIN B × 1 GOL GLYCEROL × 3 COA COENZYME A × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；290 K；16% Peg3350, 0.1 MES pH 6.5, 0.2M NH4NO3
|
Resolution 2.00 Å R-free 0.184 |
| 6QRI Structure of rabbit G-actin in complex with chivosazole A Deposited 2019-02-19 | Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 CV9 (2~{R},3~{R},5~{S},6~{E},8~{E},10~{Z},12~{S},13~{R},16~{Z},18~{E},20~{Z},22~{E},24~{R},25~{S},26~{E},28~{Z})-13-[(2~{S},3~{S},5~{S})-3,5-bis(oxidanyl)hexan-2-yl]-25-[(2~{R},3~{R},4~{S},5~{R},6~{R})-3,4-dimethoxy-6-methyl-5-oxidanyl-oxan-2-yl]oxy-3-methoxy-2,12,22,24-tetramethyl-5-oxidanyl-14,32-dioxa-33-azabicyclo[28.2.1]tritriaconta-1(33),6,8,10,16,18,20,22,26,28,30-undecaen-15-one × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；0.5 M lithium chloride, 100 mM Tris(hydroxymethyl)aminomethane hydrochloride (Tris-HCl), 28 % (w/v) polyethylenglycol 6000, pH 8.5
|
Resolution 2.40 Å R-free 0.268 |
| 6QRI Structure of rabbit G-actin in complex with chivosazole A Deposited 2019-02-19 | Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 CV9 (2~{R},3~{R},5~{S},6~{E},8~{E},10~{Z},12~{S},13~{R},16~{Z},18~{E},20~{Z},22~{E},24~{R},25~{S},26~{E},28~{Z})-13-[(2~{S},3~{S},5~{S})-3,5-bis(oxidanyl)hexan-2-yl]-25-[(2~{R},3~{R},4~{S},5~{R},6~{R})-3,4-dimethoxy-6-methyl-5-oxidanyl-oxan-2-yl]oxy-3-methoxy-2,12,22,24-tetramethyl-5-oxidanyl-14,32-dioxa-33-azabicyclo[28.2.1]tritriaconta-1(33),6,8,10,16,18,20,22,26,28,30-undecaen-15-one × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；0.5 M lithium chloride, 100 mM Tris(hydroxymethyl)aminomethane hydrochloride (Tris-HCl), 28 % (w/v) polyethylenglycol 6000, pH 8.5
|
Resolution 2.40 Å R-free 0.268 |
| 6RSW HFD domain of mouse CAP1 bound to the pointed end of G-actin Deposited 2019-05-22 | Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；0.1M HEPES, 0.1 mM KCl, 10% PEG4000 (w/v)
|
Resolution 1.95 Å R-free 0.194 |
| 6T1Y Cryo-EM structure of phalloidin-stabilized F-actin (copolymerized) Deposited 2019-10-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；5 mM Tris pH 7.5, 2 mM NaN3, 1 mM DTT, 100 mM KCl and 2 mM MgCl2, 0.01 %(v/v) MeOH, 0.02 %(v/w) Tween 20
cryo-EM vitrification conditions
Cryogen ETHANE；Manual backside blotting using Whatman filter paper No.5.
|
Resolution 3.30 Å |
| 6T20 Cryo-EM structure of phalloidin-stabilized F-actin (aged) Deposited 2019-10-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；5 mM Tris pH 7.5, 2 mM NaN3, 1 mM DTT, 100 mM KCl and 2 mM MgCl2, 2.0 %(v/v) MeOH, 0.03 %(v/w) Tween 20
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 6T23 Cryo-EM structure of jasplakinolide-stabilized F-actin (aged) Deposited 2019-10-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 PO4 PHOSPHATE ION × 5 MG MAGNESIUM ION × 5 9ZK (4~{R},7~{R},10~{S},13~{S},15~{E},19~{S})-10-(4-azanylbutyl)-4-(4-hydroxyphenyl)-7-(1~{H}-indol-3-ylmethyl)-8,13,15,19-tetramethyl-1-oxa-5,8,11-triazacyclononadec-15-ene-2,6,9,12-tetrone × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；5 mM Tris pH 7.5, 2 mM NaN3, 1 mM DTT, 100 mM KCl and 2 mM MgCl2, 0.2 %(v/v) DMSO, 0.02 %(v/w) Tween 20
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 6T24 Cryo-EM structure of jasplakinolide-stabilized F-actin (aged) Deposited 2019-10-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 PO4 PHOSPHATE ION × 5 MG MAGNESIUM ION × 5 9ZK (4~{R},7~{R},10~{S},13~{S},15~{E},19~{S})-10-(4-azanylbutyl)-4-(4-hydroxyphenyl)-7-(1~{H}-indol-3-ylmethyl)-8,13,15,19-tetramethyl-1-oxa-5,8,11-triazacyclononadec-15-ene-2,6,9,12-tetrone × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；5 mM Tris pH 7.5, 2 mM NaN3, 1 mM DTT, 100 mM KCl and 2 mM MgCl2, 0.5 %(v/v) DMSO, 0.02 %(v/w) Tween 20
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 6T25 Cryo-EM structure of phalloidin-Alexa Flour-546-stabilized F-actin (copolymerized) Deposited 2019-10-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；5 mM Tris pH 7.5, 2 mM NaN3, 1 mM DTT, 100 mM KCl and 2 mM MgCl2, 1.5 %(v/v) MeOH, 0.02 %(v/w) Tween 20
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 6U96 Actin phalloidin at BeFx state Deposited 2019-09-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；pH7.4, 10mM Tris, 50mM KCl, 1 mM MgC2, 0.2mM CaCl2, 1mM ATP, 1mM DTT, 0.2mM EGTA, 0.2mM BeCl2, 5mM NaF
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 6UBY Isolated cofilin bound to an actin filament Deposited 2019-09-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
Chain H
1–377（377 aa）
|
Not recorded | MG MAGNESIUM ION × 7 ADP ADENOSINE-5'-DIPHOSPHATE × 7 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.50 Å |
| 6UC0 Isolated S3D-cofilin bound to an actin filament Deposited 2019-09-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
|
Not recorded | MG MAGNESIUM ION × 7 ADP ADENOSINE-5'-DIPHOSPHATE × 7 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.50 Å |
| 6UC4 Barbed end side of a cofilactin cluster Deposited 2019-09-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
Chain H
1–377（377 aa）
Chain J
1–377（377 aa）
Chain K
1–377（377 aa）
Chain L
1–377（377 aa）
|
Not recorded | MG MAGNESIUM ION × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 9 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 9.20 Å |
| 6VAO Human cofilin-1 decorated actin filament Deposited 2019-12-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
|
Not recorded | MG MAGNESIUM ION × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 6VAU Bare actin filament from a partially cofilin-decorated sample Deposited 2019-12-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
|
Not recorded | MG MAGNESIUM ION × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 6VEC Cryo-EM structure of F-actin/Plastin2-ABD2 complex Deposited 2019-12-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
Chain H
1–377（377 aa）
Chain I
1–377（377 aa）
Chain J
1–377（377 aa）
Chain K
1–377（377 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 11 MG MAGNESIUM ION × 11 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 6W17 Structure of Dip1-activated Arp2/3 complex with nucleated actin filament Deposited 2020-03-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 17 PDB declaration: heptadecameric |
Chain I
1–377（377 aa）
Chain J
1–377（377 aa）
Chain K
1–377（377 aa）
Chain L
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 6W7V Structure of rabbit actin in complex with truncated analog of Mycalolide B Deposited 2020-03-19 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–377（377 aa）
|
Not recorded | CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 LAB LATRUNCULIN B × 1 EDO 1,2-ETHANEDIOL × 3 TFJ (1E,3R,4R,5S,6R,9S,10S,12S)-12-[(4-aminobutanoyl)oxy]-1-[ethyl(formyl)amino]-4,10-dimethoxy-3,5,9,13-tetramethyltetradec-1-en-6-yl (2R)-oxolane-2-carboxylate × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；0.1M Tris-HCl, pH 8.5, 25% PEG3350
|
Resolution 1.70 Å R-free 0.213 |
| 6WVT Structural basis of alphaE-catenin - F-actin catch bond behavior Deposited 2020-05-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain B
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
Chain H
1–377（377 aa）
Chain I
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 6 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.56 Å |
| 6YP9 Rabbit muscle actin in complex with ADF-H and ATP-ATTO-488 Deposited 2020-04-15 | Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；0.1 M sodium cacodylate (pH 6.0) and 15% (w/v) PEG 4000
|
Resolution 2.56 Å R-free 0.230 |
| 7AD9 Structure of the Lifeact-F-actin complex Deposited 2020-09-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric |
Chain B
1–377（377 aa）
Chain D
1–377（377 aa）
Chain F
1–377（377 aa）
Chain H
1–377（377 aa）
Chain I
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 PO4 PHOSPHATE ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8；120 mM KCl, 20 mM Tris pH 8, 2 mM MgCl2, 1 mM DTT, and 0.02% w/v Tween-20
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 7AHN Cryo-EM structure of F-actin stabilized by cis-optoJASP-8 Deposited 2020-09-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 PO4 PHOSPHATE ION × 5 RLZ ~{N}-[4-[(4~{R},7~{R},10~{S},13~{S},15~{E},19~{S})-4-(4-hydroxyphenyl)-7-(1~{H}-indol-3-ylmethyl)-8,13,15,19-tetramethyl-2,6,9,12-tetrakis(oxidanylidene)-1-oxa-5,8,11-triazacyclononadec-15-en-10-yl]butyl]-~{N}'-[5-methoxy-2-[(~{Z})-(3,4,5-trimethoxyphenyl)diazenyl]phenyl]butanediamide × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；5 mM Tris pH 7.5, 2 mM NaN3, 1 mM DTT, 100 mM KCl and 2 mM MgCl2, 0.4 %(v/v) DMSO, 0.02 %(v/w) Tween 20
cryo-EM vitrification conditions
Cryogen ETHANE；1.5 mul sample, automatic blotting for 7-7.5s, blot force -25, drain time 1s.
|
Resolution 2.90 Å |
| 7AHQ Cryo-EM structure of F-actin stabilized by trans-optoJASP-8 Deposited 2020-09-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 PO4 PHOSPHATE ION × 5 MG MAGNESIUM ION × 5 RLZ ~{N}-[4-[(4~{R},7~{R},10~{S},13~{S},15~{E},19~{S})-4-(4-hydroxyphenyl)-7-(1~{H}-indol-3-ylmethyl)-8,13,15,19-tetramethyl-2,6,9,12-tetrakis(oxidanylidene)-1-oxa-5,8,11-triazacyclononadec-15-en-10-yl]butyl]-~{N}'-[5-methoxy-2-[(~{Z})-(3,4,5-trimethoxyphenyl)diazenyl]phenyl]butanediamide × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；5 mM Tris pH 7.5, 2 mM NaN3, 1 mM DTT, 100 mM KCl and 2 mM MgCl2, 0.7 %(v/v) DMSO, 0.02 %(v/w) Tween 20
cryo-EM vitrification conditions
Cryogen ETHANE；1.5 mul sample, automatic blotting for 7-7.5s, blot force -25, drain time 1s.
|
Resolution 3.60 Å |
| 7C2F Crystal Structure of the Thorarchaeota ProGel/rabbit actin complex Deposited 2020-05-07 | Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | LAB LATRUNCULIN B × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；291 K；0.1 M MES pH 6.0
0.2 M magnesium chloride hexahydrate
20% w/v polyethylene glycol 6000
|
Resolution 2.03 Å R-free 0.231 |
| 7C2F Crystal Structure of the Thorarchaeota ProGel/rabbit actin complex Deposited 2020-05-07 | Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | LAB LATRUNCULIN B × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；291 K；0.1 M MES pH 6.0
0.2 M magnesium chloride hexahydrate
20% w/v polyethylene glycol 6000
|
Resolution 2.03 Å R-free 0.231 |
| 7C2G Crystal Structure of the Thorarchaeota 2DGel/rabbit actin complex Deposited 2020-05-07 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；291 K；0.1 M Bis-Tris pH 5.5
0.2 M magnesium chloride hexahydrate
25% w/v polyethylene glycol 3350
|
Resolution 1.71 Å R-free 0.203 |
| 7C2H Crystal Structure of the Thorarchaeota 2DGel3/rabbit actin complex Deposited 2020-05-07 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；291 K；0.1 M CHES pH 9.5
20% w/v polyethylene glycol 8000
|
Resolution 2.35 Å R-free 0.238 |
| 7CCC The structure of the actin filament uncapping complex mediated by twinfilin Deposited 2020-06-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric |
Chain A
1–377（377 aa）
Chain E
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；12% PEG 8000, 10% glycerol, 500 mM potassium chloride
|
Resolution 3.20 Å R-free 0.238 |
| 7NXV Crystal structure of the complex of DNase I/G-actin/PPP1R15A_582-621 Deposited 2021-03-19 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
3–377（375 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 5；293 K；10% PEG4000, 0.1M Acetate
|
Resolution 2.55 Å R-free 0.248 |
| 7NXV Crystal structure of the complex of DNase I/G-actin/PPP1R15A_582-621 Deposited 2021-03-19 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Other combination Heteromer；Protein × 3 PDB declaration: trimeric |
Chain D
3–377（375 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 5；293 K；10% PEG4000, 0.1M Acetate
|
Resolution 2.55 Å R-free 0.248 |
| 7NZM Cryo-EM structure of pre-dephosphorylation complex of phosphorylated eIF2alpha with trapped holophosphatase (PP1A_D64A/PPP1R15A/G-actin/DNase I) Deposited 2021-03-24 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 5 PDB declaration: pentameric |
Chain A
3–377（375 aa）
|
Not recorded | MN MANGANESE (II) ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；0.22mM Triton X-100 was added into the solution before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.96 Å |
| 7P1G Structure of the P. aeruginosa ExoY-F-actin complex Deposited 2021-07-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 15 PDB declaration: pentadecameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
|
Not recorded | MG MAGNESIUM ION × 10 GH3 3'-DEOXY-GUANOSINE-5'-TRIPHOSPHATE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 5 PO4 PHOSPHATE ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 7PLT Cryo-EM structure of the actomyosin-V complex in the rigor state (central 1er) Deposited 2021-09-01 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.30 Å |
| 7PLU Cryo-EM structure of the actomyosin-V complex in the rigor state (central 3er/2er) Deposited 2021-09-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain C
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 3 MG MAGNESIUM ION × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.20 Å |
| 7PLV Cryo-EM structure of the actomyosin-V complex in the rigor state (central 1er, class 1) Deposited 2021-09-01 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.50 Å |
| 7PLW Cryo-EM structure of the actomyosin-V complex in the rigor state (central 1er, class 2) Deposited 2021-09-01 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.50 Å |
| 7PLX Cryo-EM structure of the actomyosin-V complex in the rigor state (central 1er, class 4) Deposited 2021-09-01 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.60 Å |
| 7PLY Cryo-EM structure of the actomyosin-V complex in the rigor state (central 1er, young JASP-stabilized F-actin) Deposited 2021-09-01 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1 MG MAGNESIUM ION × 1 9UE Jasplakinolide × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.20 Å |
| 7PLZ Cryo-EM structure of the actomyosin-V complex in the rigor state (central 3er/2er, young JASP-stabilized F-actin) Deposited 2021-09-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric |
Chain C
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 3 PO4 PHOSPHATE ION × 3 MG MAGNESIUM ION × 3 9UE Jasplakinolide × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.20 Å |
| 7PM0 Cryo-EM structure of the actomyosin-V complex in the rigor state (central 1er, young JASP-stabilized F-actin, class 1) Deposited 2021-09-01 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1 MG MAGNESIUM ION × 1 9UE Jasplakinolide × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.60 Å |
| 7PM1 Cryo-EM structure of the actomyosin-V complex in the rigor state (central 1er, young JASP-stabilized F-actin, class 2) Deposited 2021-09-01 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1 MG MAGNESIUM ION × 1 9UE Jasplakinolide × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.50 Å |
| 7PM2 Cryo-EM structure of the actomyosin-V complex in the rigor state (central 1er, young JASP-stabilized F-actin, class 4) Deposited 2021-09-01 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1 MG MAGNESIUM ION × 1 9UE Jasplakinolide × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.60 Å |
| 7PM3 Cryo-EM structure of young JASP-stabilized F-actin (central 3er) Deposited 2021-09-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 3 PO4 PHOSPHATE ION × 3 MG MAGNESIUM ION × 3 9UE Jasplakinolide × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 7PM5 Cryo-EM structure of the actomyosin-V complex in the strong-ADP state (central 1er) Deposited 2021-09-02 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.10 Å |
| 7PM6 Cryo-EM structure of the actomyosin-V complex in the strong-ADP state (central 3er/2er) Deposited 2021-09-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain C
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.00 Å |
| 7PM7 Cryo-EM structure of the actomyosin-V complex in the strong-ADP state (central 1er, class 2) Deposited 2021-09-02 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.50 Å |
| 7PM8 Cryo-EM structure of the actomyosin-V complex in the strong-ADP state (central 1er, class 3) Deposited 2021-09-02 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.50 Å |
| 7PM9 Cryo-EM structure of the actomyosin-V complex in the strong-ADP state (central 1er, class 4) Deposited 2021-09-02 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.70 Å |
| 7PMA Cryo-EM structure of the actomyosin-V complex in the strong-ADP state (central 1er, class 5) Deposited 2021-09-02 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.60 Å |
| 7PMB Cryo-EM structure of the actomyosin-V complex in the strong-ADP state (central 1er, class 6) Deposited 2021-09-02 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.60 Å |
| 7PMC Cryo-EM structure of the actomyosin-V complex in the strong-ADP state (central 1er, class 7) Deposited 2021-09-02 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.70 Å |
| 7PMD Cryo-EM structure of the actomyosin-V complex in the post-rigor transition state (AppNHp, central 1er) Deposited 2021-09-02 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 MG MAGNESIUM ION × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration, two data sets combined
|
Resolution 2.90 Å |
| 7PME Cryo-EM structure of the actomyosin-V complex in the post-rigor transition state (AppNHp, central 3er/2er) Deposited 2021-09-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain C
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 MG MAGNESIUM ION × 7 ADP ADENOSINE-5'-DIPHOSPHATE × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration, two data sets combined
|
Resolution 2.90 Å |
| 7PMF Cryo-EM structure of the actomyosin-V complex in the post-rigor transition state (AppNHp, central 1er, class 1) Deposited 2021-09-02 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 MG MAGNESIUM ION × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration, two data sets combined
|
Resolution 3.40 Å |
| 7PMG Cryo-EM structure of the actomyosin-V complex in the post-rigor transition state (AppNHp, central 1er, class 3) Deposited 2021-09-02 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 MG MAGNESIUM ION × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration, two data sets combined
|
Resolution 3.30 Å |
| 7PMH Cryo-EM structure of the actomyosin-V complex in the post-rigor transition state (AppNHp, central 1er, class 4) Deposited 2021-09-02 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 MG MAGNESIUM ION × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration, two data sets combined
|
Resolution 3.40 Å |
| 7PMI Cryo-EM structure of the actomyosin-V complex in the post-rigor transition state (AppNHp, central 1er, class 5) Deposited 2021-09-02 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 MG MAGNESIUM ION × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration, two data sets combined
|
Resolution 3.30 Å |
| 7PMJ Cryo-EM structure of the actomyosin-V complex in the post-rigor transition state (AppNHp, central 1er, class 6) Deposited 2021-09-02 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 MG MAGNESIUM ION × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration, two data sets combined
|
Resolution 3.40 Å |
| 7PML Cryo-EM structure of the actomyosin-V complex in the post-rigor transition state (AppNHp, central 1er, class 8) Deposited 2021-09-02 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 MG MAGNESIUM ION × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration, two data sets combined
|
Resolution 3.30 Å |
| 7T5Q Cryo-EM Structure of a Transition State of Arp2/3 Complex Activation Deposited 2021-12-13 | Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 10 PDB declaration: decameric |
Chain H
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE；Grids were manually blotted for 3 seconds with Whatman 41 filter paper and manually plunged using a Leica EM CPC manual plunger.
|
Resolution 3.40 Å |
| 7TPT Single-particle Cryo-EM structure of Arp2/3 complex at branched-actin junction. Deposited 2022-01-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 36 PDB declaration: 36-meric |
Chain H
1–377（377 aa）
Chain I
1–377（377 aa）
Chain J
1–377（377 aa）
Chain K
1–377（377 aa）
Chain L
1–377（377 aa）
Chain M
1–377（377 aa）
Chain N
1–377（377 aa）
Chain O
1–377（377 aa）
Chain P
1–377（377 aa）
Chain Q
1–377（377 aa）
Chain R
1–377（377 aa）
Chain S
1–377（377 aa）
Chain T
1–377（377 aa）
Chain U
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 16 ADP ADENOSINE-5'-DIPHOSPHATE × 16 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 7U8K Magic Angle Spinning NMR Structure of Human Cofilin-2 Assembled on Actin Filaments Deposited 2022-03-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
Chain F
3–377（375 aa）
Chain G
3–377（375 aa）
Chain H
3–377（375 aa）
Chain I
3–377（375 aa）
Chain J
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
SOLID-STATE NMR
NMR measurement conditions
pH 6.6；273 K；Ionic strength (raw mmCIF value) 50；Pressure 1
NMR sample composition
20.8 % w/w [U-13C; U-15N] human cofilin-2, 79.2 % w/w F-actin, Solid | Solid
NMR sample composition
20.8 % w/w [1,6-13C]-glucose, U-15N human cofilin-2, 79.2 % w/w F-actin, Solid | Solid
NMR sample composition
20.8 % w/w [2-13C]-glucose, U-15N human cofilin-2, 79.2 % w/w F-actin, Solid | Solid
|
Resolution not provided |
| 7UTI ALTERNATIVE MODELING OF TROPOMYOSIN IN HUMAN CARDIAC THIN FILAMENT IN THE CALCIUM BOUND STATE Deposited 2022-04-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric |
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
Chain H
1–377（377 aa）
Chain I
1–377（377 aa）
Chain J
1–377（377 aa）
Chain K
1–377（377 aa）
Chain L
1–377（377 aa）
Chain M
1–377（377 aa）
Chain N
1–377（377 aa）
Chain O
1–377（377 aa）
Chain P
1–377（377 aa）
Chain Q
1–377（377 aa）
Chain R
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 16 MG MAGNESIUM ION × 16 CA CALCIUM ION × 6 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.80 Å |
| 7UTJ Cryogenic electron microscopy 3D map of F-actin bound by human dimeric alpha-catenin Deposited 2022-04-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 6 MG MAGNESIUM ION × 6 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.77 Å |
| 7UTL ALTERNATIVE MODELING OF TROPOMYOSIN IN HUMAN CARDIAC THIN FILAMENT IN THE CALCIUM FREE STATE Deposited 2022-04-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
Chain H
1–377（377 aa）
Chain I
1–377（377 aa）
Chain J
1–377（377 aa）
Chain K
1–377（377 aa）
Chain L
1–377（377 aa）
Chain M
1–377（377 aa）
Chain N
1–377（377 aa）
Chain O
1–377（377 aa）
Chain P
1–377（377 aa）
Chain Q
1–377（377 aa）
Chain R
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 18 MG MAGNESIUM ION × 18 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.60 Å |
| 7UUW Cryogenic electron microscopy 3D map of F-actin bound by the Actin Binding Domain of alpha-catenin ortholog, HMP1 Deposited 2022-04-29 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 6 MG MAGNESIUM ION × 6 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.36 Å |
| 7UXF Cryogenic electron microscopy 3D map of F-actin Deposited 2022-05-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 6 MG MAGNESIUM ION × 6 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 7WHF Heimdallarchaeota gelsolin (2DGel) bound to rabbit actin Deposited 2021-12-30 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 9 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；297 K；0.4 mM Heimdallarchaeota 2DGel
0.4 mM rabbit actin
1 mM CaCl2
0.1 M HEPES pH 7.0
10% w/v polyethylene glycol 6000
|
Resolution 2.10 Å R-free 0.216 |
| 7WHF Heimdallarchaeota gelsolin (2DGel) bound to rabbit actin Deposited 2021-12-30 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 9 GOL GLYCEROL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；297 K；0.4 mM Heimdallarchaeota 2DGel
0.4 mM rabbit actin
1 mM CaCl2
0.1 M HEPES pH 7.0
10% w/v polyethylene glycol 6000
|
Resolution 2.10 Å R-free 0.216 |
| 7WHG Lokiarchaeota gelsolin (2DGel) bound to two molecules of rabbit actin Deposited 2021-12-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 2 CA CALCIUM ION × 9 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；0.4 mM Loki2DGel
0.4 mM rabbit actin
0.1 M Tris-HCl, pH 7.0
0.2 M magnesium chloride hexahydrate
10% w/v polyethylene glycol 8000
1 mM CaCl2
|
Resolution 3.25 Å R-free 0.232 |
| 7Z7H Structure of P. luminescens TccC3-F-actin complex Deposited 2022-03-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 APR ADENOSINE-5-DIPHOSPHORIBOSE × 1 NCA NICOTINAMIDE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 7Z7I Structure of ADP-ribosylated F-actin Deposited 2022-03-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 APR ADENOSINE-5-DIPHOSPHORIBOSE × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 8A2R Cryo-EM structure of F-actin in the Mg2+-ADP-BeF3- nucleotide state. Deposited 2022-06-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 BEF BERYLLIUM TRIFLUORIDE ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；F-buffer:
5 mM Tris pH 7.5, 100 mM KCl, 2 mM MgCl2, 2 mM NaN3, 1 mM DTT, 0.75 mM BeF2, 5 mM NaF.
cryo-EM vitrification conditions
Cryogen ETHANE；The Vitrobot was operated at 13 degrees celsius and the samples were blotted for 9 seconds with a blot force of -25.
|
Resolution 2.17 Å |
| 8A2S Cryo-EM structure of F-actin in the Mg2+-ADP-Pi nucleotide state. Deposited 2022-06-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 PO4 PHOSPHATE ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；F-phosphate buffer:
5 mM Tris, 100 mM KCl, 2 mM MgCl2, 2 mM NaN3, 1 mM DTT, 50 mM potassium phosphate pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE；The Vitrobot was operated at 13 degrees celsius and the samples were blotted for 9 seconds with a blot force of -25.
|
Resolution 2.22 Å |
| 8A2T Cryo-EM structure of F-actin in the Mg2+-ADP nucleotide state. Deposited 2022-06-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；F-buffer:
5 mM Tris pH 7.5, 100 mM KCl, 2 mM MgCl2, 2 mM NaN3, 1 mM DTT
cryo-EM vitrification conditions
Cryogen ETHANE；The Vitrobot was operated at 13 degrees celsius and the samples were blotted for 9 seconds with a blot force of -25.
|
Resolution 2.24 Å |
| 8A2U Cryo-EM structure of F-actin in the Ca2+-ADP-BeF3- nucleotide state. Deposited 2022-06-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 CA CALCIUM ION × 5 BEF BERYLLIUM TRIFLUORIDE ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；F-buffer:
5 mM Tris pH 7.5, 100 mM KCl, 2 mM CaCl2, 2 mM NaN3, 1 mM DTT, 0.75 mM BeF2, 5 mM NaF.
cryo-EM vitrification conditions
Cryogen ETHANE；The Vitrobot was operated at 13 degrees celsius and the samples were blotted for 9 seconds with a blot force of -25.
|
Resolution 2.21 Å |
| 8A2Y Cryo-EM structure of F-actin in the Ca2+-ADP-Pi nucleotide state. Deposited 2022-06-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 CA CALCIUM ION × 5 PO4 PHOSPHATE ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；F-phosphate buffer:
5 mM Tris, 100 mM KCl, 2 mM CaCl2, 2 mM NaN3, 1 mM DTT, 50 mM potassium phosphate pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE；The Vitrobot was operated at 13 degrees celsius and the samples were blotted for 9 seconds with a blot force of -25.
|
Resolution 2.15 Å |
| 8A2Z Cryo-EM structure of F-actin in the Ca2+-ADP nucleotide state. Deposited 2022-06-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 CA CALCIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；F-buffer:
5 mM Tris pH 7.5, 100 mM KCl, 2 mM CaCl2, 2 mM NaN3, 1 mM DTT
cryo-EM vitrification conditions
Cryogen ETHANE；The Vitrobot was operated at 13 degrees celsius and the samples were blotted for 9 seconds with a blot force of -25.
|
Resolution 2.15 Å |
| 8BJH chimera of the inactive ExoY Nucleotidyl Cyclase domain from Vibrio nigripulchritudo MARTX toxin, with the double mutation K3528M and K3535I, fused to a proline-Rich-Domain (PRD) and profilin, bound to Latrunculin B-ADP-Mg-actin Deposited 2022-11-04 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 LAB LATRUNCULIN B × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 2 GOL GLYCEROL × 3 PEO HYDROGEN PEROXIDE × 8 SO4 SULFATE ION × 2 PEG DI(HYDROXYETHYL)ETHER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；30% PEG4000 0.2M LISO4 0.1M TRIS PH8.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 1.69 Å R-free 0.212 |
| 8BJI chimera of ExoY Nucleotidyl Cyclase domain from Vibrio nigripulchritudo fused to a proline-Rich-Domain (PRD) and profilin, bound to ADP-Mg-actin and a sulfate ion Deposited 2022-11-04 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 2 GOL GLYCEROL × 4 PEG DI(HYDROXYETHYL)ETHER × 2 PEO HYDROGEN PEROXIDE × 14 SO4 SULFATE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
EVAPORATION；pH 8.5；293 K；30% peg 4000, 0.2 M Lithium Sulfate (LiSO4), 0.1 M TrisHCl pH8.5
|
Resolution 1.75 Å R-free 0.212 |
| 8BJJ ExoY Nucleotidyl Cyclase domain from Vibrio nigripulchritudo MARTX toxin, bound to ATP-Mg-actin, human profilin 1 and a sulfate ion Deposited 2022-11-04 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
3–377（375 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 LAB LATRUNCULIN B × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 2 SO4 SULFATE ION × 4 PEO HYDROGEN PEROXIDE × 2 PG4 TETRAETHYLENE GLYCOL × 1 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；291 K；30% peg3000
0.3M LiSO4
0.1M Tris pH8.5
3% Dioxane
|
Resolution 1.70 Å R-free 0.195 |
| 8BO1 ExoY Nucleotidyl Cyclase domain from Vibrio nigripulchritudo MARTX toxin, bound to Latrunculin-B-ATP-Mg-actin, and 3'-DEOXYADENOSINE-5'-TRIPHOSPHATE and 2 Mg ions Deposited 2022-11-14 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
|
Not recorded | LAB LATRUNCULIN B × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 3 SO4 SULFATE ION × 1 3AT 3'-DEOXYADENOSINE-5'-TRIPHOSPHATE × 1 MN MANGANESE (II) ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；291 K；26 % peg 3350, 26 % Glycerol, 30 mM LiSO4, 0.1M TrisHCl pH8.5, 3 % Dioxane,
|
Resolution 2.50 Å R-free 0.231 |
| 8BO1 ExoY Nucleotidyl Cyclase domain from Vibrio nigripulchritudo MARTX toxin, bound to Latrunculin-B-ATP-Mg-actin, and 3'-DEOXYADENOSINE-5'-TRIPHOSPHATE and 2 Mg ions Deposited 2022-11-14 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
3–377（375 aa）
|
Not recorded | LAB LATRUNCULIN B × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 3 SO4 SULFATE ION × 2 3AT 3'-DEOXYADENOSINE-5'-TRIPHOSPHATE × 1 MN MANGANESE (II) ION × 6 AZI AZIDE ION × 6 CL CHLORIDE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；291 K；26 % peg 3350, 26 % Glycerol, 30 mM LiSO4, 0.1M TrisHCl pH8.5, 3 % Dioxane,
|
Resolution 2.50 Å R-free 0.231 |
| 8BR0 ExoY Nucleotidyl Cyclase domain from Vibrio nigripulchritudo MARTX toxin (residue Q3455 to L3863) in complex with 3'deoxyCTP and two manganese cations bound to Latrunculin-B-ADP-Mn-actin Deposited 2022-11-22 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
|
Not recorded | LAB LATRUNCULIN B × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 3 CH1 3'-DEOXY-CYTIDINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；10mg/mL binary complex in the presence of
15.2 mM 3primedCTP1.6 mM ADP, 20 mM MgCl2, 0.2 mM Latrunculin B, 23 mM KCl, 70 mM LiCl, 8 mM HEPES pH 8.5, 4 mM TCEP and mixed with 17 % PEG4000, 17 % Glycerol, 0.01 M Li2SO4, 0.1 M Tris pH 8.5, 5 mM MgCl2, 15 mM MnCl2, 1% 1-Butyl-2,3-dimethylimidazolium tetrafluoroborate (ionic liquid 18 from the Ionic Liquid Screen (Hampton Research)) in a 1:1.2 v/v hanging drop
|
Resolution 2.22 Å R-free 0.243 |
| 8BR0 ExoY Nucleotidyl Cyclase domain from Vibrio nigripulchritudo MARTX toxin (residue Q3455 to L3863) in complex with 3'deoxyCTP and two manganese cations bound to Latrunculin-B-ADP-Mn-actin Deposited 2022-11-22 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
3–377（375 aa）
|
Not recorded | LAB LATRUNCULIN B × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 3 CH1 3'-DEOXY-CYTIDINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；10mg/mL binary complex in the presence of
15.2 mM 3primedCTP1.6 mM ADP, 20 mM MgCl2, 0.2 mM Latrunculin B, 23 mM KCl, 70 mM LiCl, 8 mM HEPES pH 8.5, 4 mM TCEP and mixed with 17 % PEG4000, 17 % Glycerol, 0.01 M Li2SO4, 0.1 M Tris pH 8.5, 5 mM MgCl2, 15 mM MnCl2, 1% 1-Butyl-2,3-dimethylimidazolium tetrafluoroborate (ionic liquid 18 from the Ionic Liquid Screen (Hampton Research)) in a 1:1.2 v/v hanging drop
|
Resolution 2.22 Å R-free 0.243 |
| 8BR1 ExoY Nucleotidyl Cyclase domain from Vibrio nigripulchritudo MARTX toxin, bound to Latrunculin-B-ATP-Mg-actin, and 3'-DEOXYADENOSINE-5'-TRIPHOSPHATE and 2 Mg ions Deposited 2022-11-22 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
3–377（375 aa）
|
Not recorded | LAB LATRUNCULIN B × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 3 3AT 3'-DEOXYADENOSINE-5'-TRIPHOSPHATE × 1 SO4 SULFATE ION × 1 GOL GLYCEROL × 1 PEO HYDROGEN PEROXIDE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；26 % peg 3350, 26 % Glycerol, 30 mM LiSO4,
0.1M TrisHCl pH8.5,
3 % Dioxane,
|
Resolution 2.04 Å R-free 0.222 |
| 8BR1 ExoY Nucleotidyl Cyclase domain from Vibrio nigripulchritudo MARTX toxin, bound to Latrunculin-B-ATP-Mg-actin, and 3'-DEOXYADENOSINE-5'-TRIPHOSPHATE and 2 Mg ions Deposited 2022-11-22 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
3–377（375 aa）
|
Not recorded | LAB LATRUNCULIN B × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 3 3AT 3'-DEOXYADENOSINE-5'-TRIPHOSPHATE × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；26 % peg 3350, 26 % Glycerol, 30 mM LiSO4,
0.1M TrisHCl pH8.5,
3 % Dioxane,
|
Resolution 2.04 Å R-free 0.222 |
| 8DMX Cryo-EM structure of skeletal muscle alpha-actin Deposited 2022-07-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.37 Å |
| 8F8P Cryo-EM structure of F-actin in the ADP state Deposited 2022-11-22 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Blot force 0
Blot time 2.5 s
|
Resolution 2.26 Å |
| 8F8Q Cryo-EM structure of the CapZ-capped barbed end of F-actin Deposited 2022-11-22 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 6 MG MAGNESIUM ION × 6 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Blot force 0
Blot time 2.5 s
|
Resolution 2.79 Å |
| 8F8R Cryo-EM structure of the free barbed end of F-actin Deposited 2022-11-22 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions | Assembly 1 Protein homooligomer Homooligomer；Protein × 7 PDB declaration: heptameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 7 MG MAGNESIUM ION × 7 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Blot force 0
Blot time 2.5 s
|
Resolution 3.30 Å |
| 8F8S Cryo-EM structure of the free pointed end of F-actin Deposited 2022-11-22 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 7 PDB declaration: heptameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 7 MG MAGNESIUM ION × 7 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Blot force 0
Blot time 2.5 s
|
Resolution 2.84 Å |
| 8F8T Cryo-EM structure of the Tropomodulin-capped pointed end of F-actin Deposited 2022-11-22 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 7 MG MAGNESIUM ION × 7 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Blot force 0
Blot time 2.5 s
|
Resolution 3.26 Å |
| 8JO3 Cryo-EM structure of a Legionella effector complexed with actin and AMP Deposited 2023-06-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
1–377（377 aa）
|
Not recorded | AMP ADENOSINE MONOPHOSPHATE × 1 CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.66 Å |
| 8JO4 Cryo-EM structure of a Legionella effector complexed with actin and ATP Deposited 2023-06-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
1–377（377 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 1 CA CALCIUM ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.04 Å |
| 8OF8 Cryo-EM structure of actomyosin-5a-S1 with the full-length lever (nucleotide free) Deposited 2023-03-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain J
3–377（375 aa）
Chain K
3–377（375 aa）
Chain L
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.50 Å |
| 8PVX Structure of the Lifeact13-F-actin complex Deposited 2023-07-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.55 Å |
| 8R9V CryoEM structure of the primed actomyosin-5a complex Deposited 2023-11-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | PO4 PHOSPHATE ION × 1 MG MAGNESIUM ION × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE；F-actin was mixed with myosin-5a (S1 1 IQ motif, residues 1-797, S217A, DDEK 594-597 deletion) that had been pre-incubated with ATP, and vitrified at 10 ms post-mixing
|
Resolution 4.40 Å |
| 8RBF CryoEM structure of the post-powerstroke actomyosin-5a complex Deposited 2023-12-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE；F-actin was mixed with myosin-5a (S1 1 IQ motif, residues 1-797, S217A, DDEK 594-597 deletion) that had been pre-incubated with ATP, and vitrified at 120 ms post-mixing
|
Resolution 4.20 Å |
| 8RU0 Structure of the undecorated barbed end of F-actin. Deposited 2024-01-29 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 3 MG MAGNESIUM ION × 4 PO4 PHOSPHATE ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.1；12 mM HEPES pH 7.1, 100 mM KCl, 2.1 mM MgCl2, 1 mM EGTA, 1 mM TCEP, 0.2 mM ATP
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE；3 seconds, force 0.
|
Resolution 3.08 Å |
| 8RV2 Structure of the formin INF2 bound to the barbed end of F-actin. Deposited 2024-01-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 3 MG MAGNESIUM ION × 4 PO4 PHOSPHATE ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.1；12 mM HEPES pH 7.1, 100 mM KCl, 2.1 mM MgCl2, 1 mM EGTA, 1 mM TCEP, 0.2 mM ATP
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE；3 seconds, force 0.
|
Resolution 3.41 Å |
| 8UEE Atomic structure of Salmonella SipA/F-actin complex by cryo-EM Deposited 2023-10-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 11 PDB declaration: undecameric |
Chain F
3–377（375 aa）
Chain H
3–377（375 aa）
Chain I
3–377（375 aa）
Chain J
3–377（375 aa）
Chain K
3–377（375 aa）
Chain L
3–377（375 aa）
Chain M
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 7 MG MAGNESIUM ION × 7 PO4 PHOSPHATE ION × 7 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8；Buffer composition:
25 mM TRIS-H-Cl pH 8.0
2 mM DTT
cryo-EM vitrification conditions
Cryogen ETHANE；3 uL of sample was applied on Lacey grid, then sample was blotted for 3 seconds and plunge-froze in liquid ethane
|
Resolution 3.20 Å |
| 8UXW Arp2/3 branch junction complex, ADP state Deposited 2023-11-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric |
Chain H
1–377（377 aa）
Chain I
1–377（377 aa）
Chain M
1–377（377 aa）
Chain N
1–377（377 aa）
Chain O
1–377（377 aa）
Chain P
1–377（377 aa）
Chain Q
1–377（377 aa）
Chain R
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 9 MG MAGNESIUM ION × 10 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；The samples were incubated on the grid for 50 s and the extra solution was blotted using two Vitrobot filter papers (0.55/20 mm, Grade 595, Ted Pella) for 4 s at 0 blot force. The grids were plunged into liquid ethane at ~180 degrees C with a wait time of 0.5 s.
|
Resolution 2.70 Å |
| 8UXX Arp2/3 branch junction complex, BeFx state Deposited 2023-11-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric |
Chain H
1–377（377 aa）
Chain I
1–377（377 aa）
Chain M
1–377（377 aa）
Chain N
1–377（377 aa）
Chain O
1–377（377 aa）
Chain P
1–377（377 aa）
Chain Q
1–377（377 aa）
Chain R
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 9 MG MAGNESIUM ION × 10 BEF BERYLLIUM TRIFLUORIDE ION × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；The samples were incubated on the grid for 50 s and the extra solution was blotted using two Vitrobot filter papers (0.55/20 mm, Grade 595, Ted Pella) for 4 s at 0 blot force. The grids were plunged into liquid ethane at ~180 degrees C with a wait time of 0.5 s.
|
Resolution 3.20 Å |
| 8UZ0 Straight actin filament from Arp2/3 branch junction sample (ADP) Deposited 2023-11-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 9 PDB declaration: nonameric |
Chain J
6–377（372 aa）
Chain K
6–377（372 aa）
Chain L
6–377（372 aa）
Chain M
6–377（372 aa）
Chain N
6–377（372 aa）
Chain O
6–377（372 aa）
Chain P
6–377（372 aa）
Chain Q
6–377（372 aa）
Chain R
6–377（372 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 9 MG MAGNESIUM ION × 9 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；The samples were incubated on the grid for 50 s and the extra solution was blotted using two Vitrobot filter papers (0.55/20 mm, Grade 595, Ted Pella) for 4 s at 0 blot force. The grids were plunged into liquid ethane at ~180 degrees C with a wait time of 0.5 s.
|
Resolution 2.80 Å |
| 8UZ1 Straight actin filament from Arp2/3 branch junction sample (ADP-BeFx) Deposited 2023-11-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 9 PDB declaration: nonameric |
Chain J
6–377（372 aa）
Chain K
6–377（372 aa）
Chain L
6–377（372 aa）
Chain M
6–377（372 aa）
Chain N
6–377（372 aa）
Chain O
6–377（372 aa）
Chain P
6–377（372 aa）
Chain Q
6–377（372 aa）
Chain R
6–377（372 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 9 MG MAGNESIUM ION × 9 BEF BERYLLIUM TRIFLUORIDE ION × 9 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；The samples were incubated on the grid for 50 s and the extra solution was blotted using two Vitrobot filter papers (0.55/20 mm, Grade 595, Ted Pella) for 4 s at 0 blot force. The grids were plunged into liquid ethane at ~180 degrees C with a wait time of 0.5 s.
|
Resolution 3.60 Å |
| 8VIZ Structure of full-length gelsolin bound to the barbed end of F-actin Deposited 2024-01-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
Chain F
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 CA CALCIUM ION × 14 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.63 Å |
| 8VKH Structure of gelsolin domains G1G3 bound to the barbed end of F-actin Deposited 2024-01-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
Chain F
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 4 CA CALCIUM ION × 14 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.63 Å |
| 8W36 rabbit actin in the absence of potassium Deposited 2024-02-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
7–377（371 aa）
Chain B
7–377（371 aa）
Chain C
7–377（371 aa）
Chain D
7–377（371 aa）
Chain E
7–377（371 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.27 Å |
| 8XDL F-actin-END Deposited 2023-12-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 3 MG MAGNESIUM ION × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.44 Å |
| 8XDM F-actin-MAD Deposited 2023-12-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 3 MG MAGNESIUM ION × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.45 Å |
| 8YAE Cryo-ET structure of huntingtin actin complex Deposited 2024-02-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric |
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
Chain F
3–377（375 aa）
Chain G
3–377（375 aa）
Chain H
3–377（375 aa）
Chain I
3–377（375 aa）
Chain J
3–377（375 aa）
Chain K
3–377（375 aa）
Chain L
3–377（375 aa）
Chain M
3–377（375 aa）
Chain N
3–377（375 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 10.08 Å |
| 8YAO Cryo-ET structure of huntingtin actin dimer complex Deposited 2024-02-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric |
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
Chain F
3–377（375 aa）
Chain G
3–377（375 aa）
Chain H
3–377（375 aa）
Chain I
3–377（375 aa）
Chain J
3–377（375 aa）
Chain K
3–377（375 aa）
Chain L
3–377（375 aa）
Chain M
3–377（375 aa）
Chain N
3–377（375 aa）
Chain b
3–377（375 aa）
Chain c
3–377（375 aa）
Chain d
3–377（375 aa）
Chain e
3–377（375 aa）
Chain f
3–377（375 aa）
Chain g
3–377（375 aa）
Chain h
3–377（375 aa）
Chain i
3–377（375 aa）
Chain j
3–377（375 aa）
Chain k
3–377（375 aa）
Chain l
3–377（375 aa）
Chain m
3–377（375 aa）
Chain n
3–377（375 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 20.80 Å |
| 9AZ4 INF2 at the Barbed End of F-Actin Deposited 2024-03-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
7–377（371 aa）
Chain B
7–377（371 aa）
Chain C
7–377（371 aa）
Chain D
7–377（371 aa）
Chain E
7–377（371 aa）
Chain F
7–377（371 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 6 MG MAGNESIUM ION × 6 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.37 Å |
| 9AZ6 F-actin-Talin(R13-DD) complex Deposited 2024-03-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 13 PDB declaration: tridecameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；3.0 ul sample, blotted for 5 s from both sides with filter paper Whatman No.1
|
Resolution 2.98 Å |
| 9AZP INF2 at the Barbed End of F-Actin with Incoming Profilin-Actin Deposited 2024-03-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
7–377（371 aa）
Chain B
7–377（371 aa）
Chain C
7–377（371 aa）
Chain D
7–377（371 aa）
Chain E
7–377（371 aa）
Chain F
7–377（371 aa）
Chain I
7–377（371 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 6 MG MAGNESIUM ION × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.79 Å |
| 9AZQ INF2 at the Barbed End of F-Actin with Incoming Actin Deposited 2024-03-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric |
Chain A
7–377（371 aa）
Chain B
7–377（371 aa）
Chain C
7–377（371 aa）
Chain D
7–377（371 aa）
Chain E
7–377（371 aa）
Chain F
7–377（371 aa）
Chain I
7–377（371 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 7 MG MAGNESIUM ION × 7 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.82 Å |
| 9B03 INF2 in the Middle of F-Actin (Up state) Deposited 2024-03-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
7–377（371 aa）
Chain B
7–377（371 aa）
Chain C
7–377（371 aa）
Chain D
7–377（371 aa）
Chain E
7–377（371 aa）
Chain F
7–377（371 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 6 MG MAGNESIUM ION × 6 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.95 Å |
| 9B0K INF2 in the Middle of F-Actin (Down state) Deposited 2024-03-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
7–377（371 aa）
Chain B
7–377（371 aa）
Chain C
7–377（371 aa）
Chain D
7–377（371 aa）
Chain E
7–377（371 aa）
Chain F
7–377（371 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 6 MG MAGNESIUM ION × 6 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.03 Å |
| 9B27 Dia1 at the Barbed End of F-Actin Deposited 2024-03-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
7–377（371 aa）
Chain B
7–377（371 aa）
Chain C
7–377（371 aa）
Chain D
7–377（371 aa）
Chain E
7–377（371 aa）
Chain F
7–377（371 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 6 MG MAGNESIUM ION × 6 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.51 Å |
| 9B3D mDia1 in the middle of F-actin Deposited 2024-03-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
7–377（371 aa）
Chain B
7–377（371 aa）
Chain C
7–377（371 aa）
Chain D
7–377（371 aa）
Chain E
7–377（371 aa）
Chain F
7–377（371 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 6 MG MAGNESIUM ION × 6 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8；20mM HEPES, 50mM NaCL, 1mM EDTA, 1mM DTT, 0.05% Thesit
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.41 Å |
| 9CFU Cryo-EM structure of myosin-1c bound to F-actin in the ADP-A state Deposited 2024-06-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric |
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 4 MG MAGNESIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 9CFV Cryo-EM structure of delta-NTR myosin-1c bound to F-actin Deposited 2024-06-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric |
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 3 MG MAGNESIUM ION × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 9CFW Cryo-EM structure of myosin-1c bound to F-actin in the ADP-B state Deposited 2024-06-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric |
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 4 MG MAGNESIUM ION × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 9CFX Cryo-EM structure of myosin-1c bound to F-actin in the Rigor state Deposited 2024-06-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric |
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 3 MG MAGNESIUM ION × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 9DFS Structure of novel Myo7a-N isoform (ADP-bound) expressed in sensory hair cells (head domain + first two IQ domains), bound to F-actin Deposited 2024-08-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 4 MG MAGNESIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9DFV Structure of canonical Myo7a-C isoform (ADP-bound) expressed in sensory hair cells (head domain + first two IQ domains), bound to F-actin Deposited 2024-08-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 4 MG MAGNESIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9FJO Structure of the undecorated pointed end of F-actin Deposited 2024-05-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 4 MG MAGNESIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.1；12 mM HEPES pH 7.1, 100 mM KCl, 2.1 mM MgCl2, 1 mM EGTA, 1 mM TCEP, 0.2 mM ATP
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE；3 seconds, force 0.
|
Resolution 3.05 Å |
| 9GOB Structure of the F-tractin-F-actin complex Deposited 2024-09-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric |
Chain A
4–377（374 aa）
Chain B
4–377（374 aa）
Chain C
4–377（374 aa）
Chain D
4–377（374 aa）
Chain E
4–377（374 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.20 Å |
| 9HM9 Structure of the optimized F-tractin in complex with F-actin Deposited 2024-12-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric |
Chain A
4–377（374 aa）
Chain B
4–377（374 aa）
Chain C
4–377（374 aa）
Chain D
4–377（374 aa）
Chain E
4–377（374 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.40 Å |
| 9KBX CryoEM structure of F-actin bound with GAS2-CH3 domain. Deposited 2024-10-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric |
Chain H
7–376（370 aa）
Chain J
7–376（370 aa）
Chain K
7–376（370 aa）
Chain L
7–376（370 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 4 MG MAGNESIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8；50 mM Tris-HCl, pH 8.0, 200 mM KCl, 2 mM MgCl2, 1 mM EGTA, 4 mM DTT.
cryo-EM vitrification conditions
Cryogen ETHANE；The grid was blotted for 4 s at force 10 and plunged into ethane immediately.
|
Resolution 2.80 Å |
| 9NB9 Viral protein DP71L in complex with phosphorylated eIF2alpha (NTD) and protein phosphatase 1A (D64A), stabilized by G-actin/DNAseI Deposited 2025-02-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric |
Chain D
1–377（377 aa）
|
Not recorded | MN MANGANESE (II) ION × 1 CA CALCIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；3.5 uL volume, -5 blot force, 1.5 blot time
|
Resolution 3.03 Å |
| 9P3D cryo-EM structure of Vibrio effector VopV fragment bound to skeletal alpha F-actin Deposited 2025-06-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric |
Chain Q
6–377（372 aa）
Chain R
6–377（372 aa）
Chain S
6–377（372 aa）
Chain T
6–377（372 aa）
Chain U
6–377（372 aa）
Chain V
6–377（372 aa）
Chain W
6–377（372 aa）
Chain X
6–377（372 aa）
Chain Y
6–377（372 aa）
Chain Z
6–377（372 aa）
Chain a
6–377（372 aa）
|
Not recorded | MG MAGNESIUM ION × 11 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 11 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 9Q7K Pointed end of Cofilin-2 Bound F-actin Deposited 2025-08-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
2–377（376 aa）
Chain B
2–377（376 aa）
Chain C
2–377（376 aa）
Chain D
2–377（376 aa）
Chain E
2–377（376 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.13 Å |
| 9Q7L Barbed end of F-actin and cofilin on sides Deposited 2025-08-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
2–377（376 aa）
Chain B
2–377（376 aa）
Chain C
2–377（376 aa）
Chain D
2–377（376 aa）
Chain E
2–377（376 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.68 Å |
| 9Q7M Barbed end of cofilin actin, cofilin on second-to-last barbed end subunit Deposited 2025-08-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric |
Chain A
2–377（376 aa）
Chain B
2–377（376 aa）
Chain C
2–377（376 aa）
Chain D
2–377（376 aa）
Chain E
2–377（376 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 9Q7N Cofilin barbed end, cofilin on the two barbed end subunits Deposited 2025-08-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
2–377（376 aa）
Chain B
2–377（376 aa）
Chain C
2–377（376 aa）
Chain D
2–377（376 aa）
Chain E
2–377（376 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.48 Å |
| 9Q7O One CAP-1 Bound to the Pointed End of F-actin Deposited 2025-08-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric |
Chain A
2–377（376 aa）
Chain B
2–377（376 aa）
Chain C
2–377（376 aa）
Chain D
2–377（376 aa）
Chain E
2–377（376 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.02 Å |
| 9UG2 Severed and capped actin fragment by two G1G3 domains of gelsolin Deposited 2025-04-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric |
Chain C
7–376（370 aa）
Chain D
7–376（370 aa）
Chain E
7–376（370 aa）
Chain F
7–376（370 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 4 MG MAGNESIUM ION × 5 PO4 PHOSPHATE ION × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.89 Å |
| 9XYE Two CAP-1 Bound to the Pointed End of F-actin Deposited 2025-08-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric |
Chain A
2–377（376 aa）
Chain B
2–377（376 aa）
Chain C
2–377（376 aa）
Chain D
2–377（376 aa）
Chain E
2–377（376 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.18 Å |
| 9Y52 One CAP-1 Bound to the Pointed End of Cofilin F-actin Deposited 2025-09-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 11 PDB declaration: undecameric |
Chain A
2–377（376 aa）
Chain B
2–377（376 aa）
Chain C
2–377（376 aa）
Chain D
2–377（376 aa）
Chain E
2–377（376 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.46 Å |
| 9Y9J Two CAP-1 Bound to the Pointed End of Cofilin F-actin Deposited 2025-09-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain A
2–377（376 aa）
Chain B
2–377（376 aa）
Chain C
2–377（376 aa）
Chain D
2–377（376 aa）
Chain E
2–377（376 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.58 Å |
| 9Y9L CP at the barbed end with one cofilin on second-to-last subunit Deposited 2025-09-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 11 PDB declaration: 11-meric |
Chain A
2–377（376 aa）
Chain B
2–377（376 aa）
Chain C
2–377（376 aa）
Chain D
2–377（376 aa）
Chain E
2–377（376 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.06 Å |
| 9Y9M Capping protein bound to the barbed end of cofilactin Deposited 2025-09-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
2–377（376 aa）
Chain B
2–377（376 aa）
Chain C
2–377（376 aa）
Chain D
2–377（376 aa）
Chain E
2–377（376 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.06 Å |
| 9Y9P Cofilactin filament Deposited 2025-09-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
2–377（376 aa）
Chain B
2–377（376 aa）
Chain C
2–377（376 aa）
Chain D
2–377（376 aa）
Chain E
2–377（376 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.06 Å |
| 9YIM Capping protein bound to the barbed end of F-actin Deposited 2025-10-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric |
Chain A
7–377（371 aa）
Chain B
7–377（371 aa）
Chain C
7–377（371 aa）
Chain D
7–377（371 aa）
Chain E
7–377（371 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.62 Å |
293 other PDB entries and 353 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | ACTS_RABIT |
| Isoform | — |
| PDB entities | 1 |
| Chains and sequence ranges | Author chain A； PDBConstruct 1–375； UniProt 3–377 |
