|
1EQY
COMPLEX BETWEEN RABBIT MUSCLE ALPHA-ACTIN: HUMAN GELSOLIN DOMAIN 1
Deposited 2000-04-06
|
Different construct
Different mutation/modification
Different oligomeric state
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain A
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
CA CALCIUM ION × 3
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 6.6；298 K；PEG 6000, sodium chloride, adenosine triphosphate, calcium, magnesium, sodium azide, pH 6.6, Vapor Diffusion, temperature 298.0K
|
Resolution 2.30 Å
R-free 0.280
|
|
1ESV
COMPLEX BETWEEN LATRUNCULIN A:RABBIT MUSCLE ALPHA ACTIN:HUMAN GELSOLIN DOMAIN 1
Deposited 2000-04-11
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain A
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
CA CALCIUM ION × 3
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
LAR LATRUNCULIN A × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 6.6；298 K；PEG 6000, sodium Chloride, adenosine triphosphate, calcium, magnesium, sodium azide, pH 6.6, VAPOR DIFFUSION, temperature 298.0K
|
Resolution 2.00 Å
R-free 0.286
|
|
1IJJ
THE X-RAY CRYSTAL STRUCTURE OF THE COMPLEX BETWEEN RABBIT SKELETAL MUSCLE ACTIN AND LATRUNCULIN A AT 2.85 A RESOLUTION
Deposited 2001-04-26
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain A
1–377（377 aa）
|
Not recorded
|
MG MAGNESIUM ION × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
LAR LATRUNCULIN A × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；298 K；ammonium sulfate, magnesium chloride, pH 6.8,
VAPOR DIFFUSION, HANGING DROP at 298 K
|
Resolution 2.85 Å
R-free 0.309
|
|
1IJJ
THE X-RAY CRYSTAL STRUCTURE OF THE COMPLEX BETWEEN RABBIT SKELETAL MUSCLE ACTIN AND LATRUNCULIN A AT 2.85 A RESOLUTION
Deposited 2001-04-26
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain B
1–377（377 aa）
|
Not recorded
|
MG MAGNESIUM ION × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
LAR LATRUNCULIN A × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；298 K；ammonium sulfate, magnesium chloride, pH 6.8,
VAPOR DIFFUSION, HANGING DROP at 298 K
|
Resolution 2.85 Å
R-free 0.309
|
|
1J6Z
UNCOMPLEXED ACTIN
Deposited 2001-05-15
|
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
CA CALCIUM ION × 6
ADP ADENOSINE-5'-DIPHOSPHATE × 1
RHO TETRAMETHYLRHODAMINE-5-MALEIMIDE × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；PEG 2000 MONOMETHYLETHER 22%, CALCIUM ACETATE 200mM, TRIS 10mM (pH 7), pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.54 Å
R-free 0.223
|
|
1KXP
CRYSTAL STRUCTURE OF HUMAN VITAMIN D-BINDING PROTEIN IN COMPLEX WITH SKELETAL ACTIN
Deposited 2002-02-01
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain A
3–377（375 aa）
|
Not recorded
|
MG MAGNESIUM ION × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.6；277 K；Peg 8K 12%, Magnesium Acetate 200mM, Sodium Cacodylate 100 mM, Glycerol 20%, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.10 Å
R-free 0.236
|
|
1LCU
Polylysine Induces an Antiparallel Actin Dimer that Nucleates Filament Assembly: Crystal Structure at 3.5 A Resolution
Deposited 2002-04-06
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 2
PDB declaration: dimeric
|
Chain A
7–377（371 aa）
Chain B
7–377（371 aa）
|
Not recorded
|
CA CALCIUM ION × 4
CL CHLORIDE ION × 3
ATP ADENOSINE-5'-TRIPHOSPHATE × 2
LAR LATRUNCULIN A × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；Ammonium sulfate, MgCl2, Imidazole, ATP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.50 Å
R-free 0.266
|
|
1LOT
CRYSTAL STRUCTURE OF THE COMPLEX OF ACTIN WITH VITAMIN D-BINDING PROTEIN
Deposited 2002-05-07
|
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain B
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
GOL GLYCEROL × 6
CA CALCIUM ION × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；290 K；PEG8000, cacodylate, calcium acetate, glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K
|
Resolution 2.50 Å
R-free 0.279
|
|
1M8Q
Molecular Models of Averaged Rigor Crossbridges from Tomograms of Insect Flight Muscle
Deposited 2002-07-25
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 26
PDB declaration: 26-meric
|
Chain 0
1–375（375 aa）
Chain 1
1–375（375 aa）
Chain 2
1–375（375 aa）
Chain 3
1–375（375 aa）
Chain 4
1–375（375 aa）
Chain 5
1–375（375 aa）
Chain 7
1–375（375 aa）
Chain 8
1–375（375 aa）
Chain 9
1–375（375 aa）
Chain V
1–375（375 aa）
Chain W
1–375（375 aa）
Chain X
1–375（375 aa）
Chain Y
1–375（375 aa）
Chain Z
1–375（375 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM vitrification conditions
No vitrification. Samples were viewed at room temperature.
|
Resolution 70.00 Å
|
|
1MA9
Crystal structure of the complex of human vitamin D binding protein and rabbit muscle actin
Deposited 2002-08-02
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain B
3–372（370 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
MG MAGNESIUM ION × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.3；277 K；PEG 8000, magnesium acetate, sodium cacodylate, glycerol, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.40 Å
R-free 0.250
|
|
1MVW
MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE
Deposited 2002-09-26
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 32
PDB declaration: 32-meric
|
Chain 1
3–377（375 aa）
Chain 2
3–377（375 aa）
Chain 3
3–377（375 aa）
Chain 4
3–377（375 aa）
Chain 5
3–377（375 aa）
Chain 6
3–377（375 aa）
Chain 7
3–377（375 aa）
Chain 8
3–377（375 aa）
Chain 9
3–377（375 aa）
Chain V
3–377（375 aa）
Chain W
3–377（375 aa）
Chain X
3–377（375 aa）
Chain Y
3–377（375 aa）
Chain Z
3–377（375 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM vitrification conditions
NO VITRIFICATION. SAMPLES WERE VIEWED AT ROOM TEMPERATURE.
|
Resolution 70.00 Å
|
|
1NWK
CRYSTAL STRUCTURE OF MONOMERIC ACTIN IN THE ATP STATE
Deposited 2003-02-06
|
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
CA CALCIUM ION × 4
ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1
RHO TETRAMETHYLRHODAMINE-5-MALEIMIDE × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；PEG 3350, CaCl2, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 393K
|
Resolution 1.85 Å
R-free 0.226
|
|
1O18
MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE
Deposited 2002-11-04
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 30
PDB declaration: 30-meric
|
Chain 1
3–377（375 aa）
Chain 2
3–377（375 aa）
Chain 3
3–377（375 aa）
Chain 4
3–377（375 aa）
Chain 5
3–377（375 aa）
Chain 6
3–377（375 aa）
Chain 7
3–377（375 aa）
Chain 8
3–377（375 aa）
Chain 9
3–377（375 aa）
Chain V
3–377（375 aa）
Chain W
3–377（375 aa）
Chain X
3–377（375 aa）
Chain Y
3–377（375 aa）
Chain Z
3–377（375 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM vitrification conditions
NO VITRIFICATION. SAMPLES WERE VIEWED AT ROOM TEMPERATURE.
|
Resolution 70.00 Å
|
|
1O19
MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE
Deposited 2002-11-15
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 32
PDB declaration: 32-meric
|
Chain 1
3–377（375 aa）
Chain 2
3–377（375 aa）
Chain 3
3–377（375 aa）
Chain 4
3–377（375 aa）
Chain 5
3–377（375 aa）
Chain 6
3–377（375 aa）
Chain 7
3–377（375 aa）
Chain 8
3–377（375 aa）
Chain 9
3–377（375 aa）
Chain V
3–377（375 aa）
Chain W
3–377（375 aa）
Chain X
3–377（375 aa）
Chain Y
3–377（375 aa）
Chain Z
3–377（375 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM vitrification conditions
NO VITRIFICATION. SAMPLES WERE VIEWED AT ROOM TEMPERATURE.
|
Resolution 70.00 Å
|
|
1O1A
MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE
Deposited 2002-11-18
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 32
PDB declaration: 32-meric
|
Chain 1
3–377（375 aa）
Chain 2
3–377（375 aa）
Chain 3
3–377（375 aa）
Chain 4
3–377（375 aa）
Chain 5
3–377（375 aa）
Chain 6
3–377（375 aa）
Chain 7
3–377（375 aa）
Chain 8
3–377（375 aa）
Chain 9
3–377（375 aa）
Chain V
3–377（375 aa）
Chain W
3–377（375 aa）
Chain X
3–377（375 aa）
Chain Y
3–377（375 aa）
Chain Z
3–377（375 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM vitrification conditions
NO VITRIFICATION. SAMPLES WERE VIEWED AT ROOM TEMPERATURE.
|
Resolution 70.00 Å
|
|
1O1B
MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE
Deposited 2002-11-15
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 26
PDB declaration: 26-meric
|
Chain 0
3–377（375 aa）
Chain 1
3–377（375 aa）
Chain 2
3–377（375 aa）
Chain 3
3–377（375 aa）
Chain 4
3–377（375 aa）
Chain 5
3–377（375 aa）
Chain 7
3–377（375 aa）
Chain 8
3–377（375 aa）
Chain 9
3–377（375 aa）
Chain V
3–377（375 aa）
Chain W
3–377（375 aa）
Chain X
3–377（375 aa）
Chain Y
3–377（375 aa）
Chain Z
3–377（375 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM vitrification conditions
NO VITRIFICATION. SAMPLES WERE VIEWED AT ROOM TEMPERATURE.
|
Resolution 70.00 Å
|
|
1O1C
MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE
Deposited 2002-11-18
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 29
PDB declaration: 29-meric
|
Chain 0
3–377（375 aa）
Chain 1
3–377（375 aa）
Chain 2
3–377（375 aa）
Chain 3
3–377（375 aa）
Chain 4
3–377（375 aa）
Chain 5
3–377（375 aa）
Chain 7
3–377（375 aa）
Chain 8
3–377（375 aa）
Chain 9
3–377（375 aa）
Chain V
3–377（375 aa）
Chain W
3–377（375 aa）
Chain X
3–377（375 aa）
Chain Y
3–377（375 aa）
Chain Z
3–377（375 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM vitrification conditions
NO VITRIFICATION. SAMPLES WERE VIEWED AT ROOM TEMPERATURE.
|
Resolution 70.00 Å
|
|
1O1D
MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE
Deposited 2002-11-18
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 32
PDB declaration: 32-meric
|
Chain 0
3–377（375 aa）
Chain 1
3–377（375 aa）
Chain 2
3–377（375 aa）
Chain 3
3–377（375 aa）
Chain 4
3–377（375 aa）
Chain 5
3–377（375 aa）
Chain 7
3–377（375 aa）
Chain 8
3–377（375 aa）
Chain 9
3–377（375 aa）
Chain V
3–377（375 aa）
Chain W
3–377（375 aa）
Chain X
3–377（375 aa）
Chain Y
3–377（375 aa）
Chain Z
3–377（375 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM vitrification conditions
NO VITRIFICATION. SAMPLES WERE VIEWED AT ROOM TEMPERATURE.
|
Resolution 70.00 Å
|
|
1O1E
MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE
Deposited 2002-11-19
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 32
PDB declaration: 32-meric
|
Chain 1
3–377（375 aa）
Chain 2
3–377（375 aa）
Chain 3
3–377（375 aa）
Chain 4
3–377（375 aa）
Chain 5
3–377（375 aa）
Chain 6
3–377（375 aa）
Chain 7
3–377（375 aa）
Chain 8
3–377（375 aa）
Chain 9
3–377（375 aa）
Chain V
3–377（375 aa）
Chain W
3–377（375 aa）
Chain X
3–377（375 aa）
Chain Y
3–377（375 aa）
Chain Z
3–377（375 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM vitrification conditions
NO VITRIFICATION. SAMPLES WERE VIEWED AT ROOM TEMPERATURE.
|
Resolution 70.00 Å
|
|
1O1F
MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE
Deposited 2002-11-15
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 26
PDB declaration: 26-meric
|
Chain 0
3–377（375 aa）
Chain 1
3–377（375 aa）
Chain 2
3–377（375 aa）
Chain 3
3–377（375 aa）
Chain 4
3–377（375 aa）
Chain 5
3–377（375 aa）
Chain 6
3–377（375 aa）
Chain 7
3–377（375 aa）
Chain 8
3–377（375 aa）
Chain V
3–377（375 aa）
Chain W
3–377（375 aa）
Chain X
3–377（375 aa）
Chain Y
3–377（375 aa）
Chain Z
3–377（375 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM vitrification conditions
NO VITRIFICATION. SAMPLES WERE VIEWED AT ROOM TEMPERATURE.
|
Resolution 70.00 Å
|
|
1O1G
MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE
Deposited 2002-11-19
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 32
PDB declaration: 32-meric
|
Chain 1
3–377（375 aa）
Chain 2
3–377（375 aa）
Chain 3
3–377（375 aa）
Chain 4
3–377（375 aa）
Chain 5
3–377（375 aa）
Chain 6
3–377（375 aa）
Chain 7
3–377（375 aa）
Chain 8
3–377（375 aa）
Chain 9
3–377（375 aa）
Chain V
3–377（375 aa）
Chain W
3–377（375 aa）
Chain X
3–377（375 aa）
Chain Y
3–377（375 aa）
Chain Z
3–377（375 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM vitrification conditions
NO VITRIFICATION. SAMPLES WERE VIEWED AT ROOM TEMPERATURE.
|
Resolution 70.00 Å
|
|
1P8Z
Complex Between Rabbit Muscle alpha-Actin: Human Gelsolin Residues Val26-Glu156
Deposited 2003-05-08
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain A
1–377（377 aa）
|
Not recorded
|
CD CADMIUM ION × 3
CA CALCIUM ION × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；293 K；0.1M Sodium Acetate, 10 mM Cadmium Chloride, 12.5% (v/v) PEG 400, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.60 Å
R-free 0.263
|
|
1P8Z
Complex Between Rabbit Muscle alpha-Actin: Human Gelsolin Residues Val26-Glu156
Deposited 2003-05-08
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Protein heterocomplex
Heteromer；Protein × 4
PDB declaration: tetrameric
|
Chain A
1–377（377 aa）
|
Not recorded
|
CD CADMIUM ION × 6
CA CALCIUM ION × 2
ATP ADENOSINE-5'-TRIPHOSPHATE × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；293 K；0.1M Sodium Acetate, 10 mM Cadmium Chloride, 12.5% (v/v) PEG 400, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.60 Å
R-free 0.263
|
|
1QZ5
Structure of rabbit actin in complex with kabiramide C
Deposited 2003-09-15
|
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
CA CALCIUM ION × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
KAB KABIRAMIDE C × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
Batch；pH 5.5；293 K；Crystals of the actin-kabiramide C complex were grown by small-scale batch by mixing equal volumes of the complex and 100 mM MES, 18% (w/v) polyethylene glycol 1500, 12% (w/v) 1,6-hexanediol, 100 mM CaCl2, 1 mM sodium azide, 1 mM TCEP, pH 5.5, Batch, temperature 293K
|
Resolution 1.45 Å
R-free 0.187
|
|
1QZ6
Structure of rabbit actin in complex with jaspisamide A
Deposited 2003-09-15
|
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Other combination
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
JAS JASPISAMIDE A × 1
CA CALCIUM ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
Batch；pH 7；293 K；Crystals were grown by small-scale batch by mixing equal volumes of the complex (10 mg/ml protein) and 100 mM bis-tris propane, 15% (w/v) dimethyl polyethylene glycol 5000, 25 mM CaCl2, 1 mM sodium azide, 1 mM TCEP, pH 7.0, Batch, temperature 293K
|
Resolution 1.60 Å
R-free 0.197
|
|
1RDW
Actin Crystal Dynamics: Structural Implications for F-actin Nucleation, Polymerization and Branching Mediated by the Anti-parallel Dimer
Deposited 2003-11-06
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 2
PDB declaration: dimeric
|
Chain X
3–377（375 aa）
Fragment：ACtin
|
Not recorded
|
MG MAGNESIUM ION × 4
ATP ADENOSINE-5'-TRIPHOSPHATE × 2
LAR LATRUNCULIN A × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.7；MgCl2, NH4SO4, pH 6.7, VAPOR DIFFUSION, HANGING DROP
|
Resolution 2.30 Å
R-free 0.224
|
|
1RFQ
Actin Crystal Dynamics: Structural Implications for F-actin Nucleation, Polymerization and Branching Mediated by the Anti-parallel Dimer
Deposited 2003-11-10
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 2
PDB declaration: dimeric
|
Chain A
3–377（375 aa）
Fragment：actin
Chain B
3–377（375 aa）
Fragment：actin
|
Not recorded
|
MG MAGNESIUM ION × 2
ATP ADENOSINE-5'-TRIPHOSPHATE × 2
LAR LATRUNCULIN A × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.7；MgCl2, NH4SO4, pH 6.7, VAPOR DIFFUSION, HANGING DROP
|
Resolution 3.00 Å
R-free 0.261
|
|
1RGI
Crystal structure of gelsolin domains G1-G3 bound to actin
Deposited 2003-11-12
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain A
1–377（377 aa）
|
Not recorded
|
CA CALCIUM ION × 4
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；277 K；2% PEG 8000, 100 mM Sodium acetate, 1 mM CaCl2, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 3.00 Å
R-free 0.258
|
|
1S22
Absolute Stereochemistry of Ulapualide A
Deposited 2004-01-07
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain A
1–375（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
CA CALCIUM ION × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
ULA ULAPUALIDE A × 1
EDO 1,2-ETHANEDIOL × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
Batch；pH 6；293 K；100 mM MES, 15% methyl ether poly(ethylene glycol) 5000, 75 mM CaCl2, 1 mM sodium azide, 1 mM TCEP, pH 6.0, Batch, temperature 293K
|
Resolution 1.60 Å
R-free 0.179
|
|
1SQK
CRYSTAL STRUCTURE OF CIBOULOT IN COMPLEX WITH SKELETAL ACTIN
Deposited 2004-03-19
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain A
1–377（377 aa）
|
Not recorded
|
MG MAGNESIUM ION × 1
ADP ADENOSINE-5'-DIPHOSPHATE × 1
LAR LATRUNCULIN A × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；PEG 8000 20%, MAGNESIUM ACETATE 200MM, SODIUM CACODYLATE 100 MM, pH 6.50, VAPOR DIFFUSION, HANGING DROP
|
Resolution 2.50 Å
R-free 0.278
|
|
1WUA
The structure of Aplyronine A-actin complex
Deposited 2004-12-03
|
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
CA CALCIUM ION × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
AP8 (8R,9R,10R,11R,14S,18S,20S,24S)-24-{(1R,2S,3R,6R,7R,8R,9S,10E)-8-(ACETYLOXY)-6-[(N,N-DIMETHYLALANYL)OXY]-11-[FORMYL(MET HYL)AMINO]-2-HYDROXY-1,3,7,9-TETRAMETHYLUNDEC-10-ENYL}-10-HYDROXY-14,20-DIMETHOXY-9,11,15,18-TETRAMETHYL-2-OXOOXACYCLOTE TRACOSA-3,5,15,21-TETRAEN-8-YL N,N,O-TRIMETHYLSERINATE × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；PEG8000 20%(w/v) 0.1M sodium cacodylate , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.45 Å
R-free 0.176
|
|
1Y64
Bni1p Formin Homology 2 Domain complexed with ATP-actin
Deposited 2004-12-03
|
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
CA CALCIUM ION × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.6；298 K；potassium bromide, hepes, DTT, Tris, ATP, calcium chloride, ethylene glycol, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.05 Å
R-free 0.313
|
|
1YXQ
Crystal structure of actin in complex with swinholide A
Deposited 2005-02-22
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 2
PDB declaration: dimeric
|
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
MG MAGNESIUM ION × 3
ATP ADENOSINE-5'-TRIPHOSPHATE × 2
EDO 1,2-ETHANEDIOL × 2
SWI SWINHOLIDE A × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
small-scale batch；pH 8.5；277 K；dimethyl PEG 5000, HEPPS, MgCl2, TCEP, NaN3, pH 8.5, small-scale batch, temperature 277K
|
Resolution 2.01 Å
R-free 0.219
|
|
2A3Z
Ternary complex of the WH2 domain of WASP with Actin-DNAse I
Deposited 2005-06-27
|
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Other combination
Heteromer；Protein × 3
PDB declaration: trimeric
|
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
CA CALCIUM ION × 2
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
GOL GLYCEROL × 3
MG MAGNESIUM ION × 1
FMT FORMIC ACID × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.2；293 K；sodium formate, PEG2000 MME, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.08 Å
R-free 0.210
|
|
2A40
Ternary complex of the WH2 domain of WAVE with Actin-DNAse I
Deposited 2005-06-27
|
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Other combination
Heteromer；Protein × 3
PDB declaration: trimeric
|
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
CA CALCIUM ION × 2
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
GOL GLYCEROL × 2
MG MAGNESIUM ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.6；293 K；sodium formate, PEG3350, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.80 Å
R-free 0.215
|
|
2A40
Ternary complex of the WH2 domain of WAVE with Actin-DNAse I
Deposited 2005-06-27
|
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Other combination
Heteromer；Protein × 3
PDB declaration: trimeric
|
Chain D
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
CA CALCIUM ION × 2
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.6；293 K；sodium formate, PEG3350, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.80 Å
R-free 0.215
|
|
2A40
Ternary complex of the WH2 domain of WAVE with Actin-DNAse I
Deposited 2005-06-27
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 3
Other combination
Heteromer；Protein × 6
PDB declaration: hexameric
|
Chain A
3–377（375 aa）
Chain D
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
CA CALCIUM ION × 4
ATP ADENOSINE-5'-TRIPHOSPHATE × 2
GOL GLYCEROL × 2
MG MAGNESIUM ION × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.6；293 K；sodium formate, PEG3350, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.80 Å
R-free 0.215
|
|
2A41
Ternary complex of the WH2 Domain of WIP with Actin-DNAse I
Deposited 2005-06-27
|
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Other combination
Heteromer；Protein × 3
PDB declaration: trimeric
|
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
CA CALCIUM ION × 2
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 1
FMT FORMIC ACID × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；sodium formate, PEG3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.60 Å
R-free 0.219
|
|
2A42
Actin-DNAse I Complex
Deposited 2005-06-27
|
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Other combination
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
CA CALCIUM ION × 2
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
GOL GLYCEROL × 3
MG MAGNESIUM ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；sodium formate, PEG3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.85 Å
R-free 0.199
|
|
2A5X
Crystal Structure of a Cross-linked Actin Dimer
Deposited 2005-07-01
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 2
PDB declaration: dimeric
|
Chain A
3–377（375 aa）
|
Not recorded
|
CA CALCIUM ION × 4
ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2
LAR LATRUNCULIN A × 2
NSB N,N,N-TRIMETHYL-3-SULFOPROPAN-1-AMINIUM × 2
MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.7；293 K；35% MPD, 100 mM sodium acetate pH 4.7, 20 mM calcium chloride, non-detergent sulfo-betaine, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.49 Å
R-free 0.250
|
|
2ASM
Structure of Rabbit Actin In Complex With Reidispongiolide A
Deposited 2005-08-23
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain A
3–377（375 aa）
|
Not recorded
|
CA CALCIUM ION × 2
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
RGA REIDISPONGIOLIDE A × 1
EDO 1,2-ETHANEDIOL × 5
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；100mM Na/MES/Acetate, pH 5.5, 7% methyl ether poly(ethylene glycol) 5000, 100mM CaCl2, 1mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.60 Å
R-free 0.189
|
|
2ASO
Structure of Rabbit Actin In Complex With Sphinxolide B
Deposited 2005-08-23
|
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
CA CALCIUM ION × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
SPX SPHINXOLIDE B × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；100mM Na/MES/Acetate, pH 5.5, 12% methyl ether poly(ethylene glycol) 5000, 100mM CaCl2, 1mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.70 Å
R-free 0.213
|
|
2ASP
Structure of Rabbit Actin In Complex With Reidispongiolide C
Deposited 2005-08-23
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain A
3–377（375 aa）
|
Not recorded
|
CA CALCIUM ION × 2
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
RGC REIDISPONGIOLIDE C × 1
EDO 1,2-ETHANEDIOL × 5
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；50mM MES, pH 6.5, 12% methyl ether poly(ethylene glycol) 5000, 40mM MgCl2, 10% ethylene glycol, 1mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.64 Å
R-free 0.193
|
|
2D1K
Ternary complex of the WH2 domain of mim with actin-dnase I
Deposited 2005-08-26
|
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Other combination
Heteromer；Protein × 3
PDB declaration: trimeric
|
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
CA CALCIUM ION × 2
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；sodium formate, PEG3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.50 Å
R-free 0.284
|
|
2FF6
Crystal structure of Gelsolin domain 1:ciboulot domain 2 hybrid in complex with actin
Deposited 2005-12-19
|
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 3
PDB declaration: trimeric
|
Chain A
3–377（375 aa）
|
Not recorded
|
CA CALCIUM ION × 3
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293.15 K；7% PEG 3000, 0.1M Hepes, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K
|
Resolution 2.05 Å
R-free 0.237
|
|
2FXU
X-ray Structure of Bistramide A- Actin Complex at 1.35 A resolution.
Deposited 2006-02-06
|
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
CA CALCIUM ION × 4
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
BID BISTRAMIDE A × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 6；298 K；The bistramide A- actin complex was mixed with the crystallization buffer in 1:1 ratio. The crystallization buffer is 100 mM MES (ph 6.0), 24% (w/v) PEG1500, 70 mM CaCl2, 1mM NaN3, 1mM TCEP., VAPOR DIFFUSION, temperature 298K
|
Resolution 1.35 Å
R-free 0.201
|
|
2GWJ
SpvB ADP-ribosylated actin: hexagonal crystal form
Deposited 2006-05-04
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain A
7–377（371 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
CA CALCIUM ION × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
|
X-RAY DIFFRACTION
mmCIF provides none of the parsed conditions
|
Resolution 1.90 Å
R-free 0.184
|
|
2GWK
SpvB ADP-ribosylated actin: orthorhombic crystal form
Deposited 2006-05-04
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 2
PDB declaration: dimeric
|
Chain A
7–377（371 aa）
Chain B
7–377（371 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
CA CALCIUM ION × 2
ATP ADENOSINE-5'-TRIPHOSPHATE × 2
|
X-RAY DIFFRACTION
mmCIF provides none of the parsed conditions
|
Resolution 2.00 Å
R-free 0.211
|
|
2HMP
Uncomplexed actin cleaved with protease ECP32
Deposited 2006-07-11
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 2
PDB declaration: dimeric
|
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
SR STRONTIUM ION × 7
SPD SPERMIDINE × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 2
EDO 1,2-ETHANEDIOL × 9
211 2,2',2''-NITRILOTRIETHANOL × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.75；277 K；1:1 mixture of 5-7.5 mg/ml protein solution containing 0.5 mM ATP and
precipitant solution (40 mM SrCl2, 10% ethylene glycol, 13-15%
dimethyl polyethylene glycol 5000, 50 mM triethanolamine, 10 mM
spermidine, pH 7.75) equilibrated against 0.5 ml precipitant., VAPOR
DIFFUSION, HANGING DROP, temperature 277K, VAPOR DIFFUSION, HANGING DROP
|
Resolution 1.90 Å
R-free 0.215
|
|
2PAV
Ternary complex of Profilin-Actin with the Last Poly-Pro of Human VASP
Deposited 2007-03-27
|
Different mutation/modification
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 3
PDB declaration: trimeric
|
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
CA CALCIUM ION × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；277.15 K；200 mM sodium formate, 20% PEG 3350, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K
|
Resolution 1.80 Å
R-free 0.208
|
|
2PBD
Ternary complex of profilin-actin with the poly-PRO-GAB domain of VASP*
Deposited 2007-03-28
|
Different construct
Different mutation/modification
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 3
PDB declaration: trimeric
|
Chain A
1–377（377 aa）
Fragment：residues 1-377
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
CA CALCIUM ION × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；277.15 K；150mM DL-malic acid pH 7.0, 18% PEG 3350, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K
|
Resolution 1.50 Å
R-free 0.190
|
|
2Q0R
Structure of Pectenotoxin-2 Bound to Actin
Deposited 2007-05-22
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain A
3–377（375 aa）
|
Not recorded
|
CA CALCIUM ION × 4
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
PXT PECTENOTOXIN-2 × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；100 mM Na/MES/acetate, pH 5.5, 15% methyl ether poly(ethylene glycol) 5000, 10% hexanediol, 30 mM CaCl2, and 1 mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.70 Å
R-free 0.204
|
|
2Q0U
Structure of Pectenotoxin-2 and Latrunculin B Bound to Actin
Deposited 2007-05-22
|
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
CA CALCIUM ION × 2
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
LAB LATRUNCULIN B × 1
PXT PECTENOTOXIN-2 × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；100 mM Na/MES/acetate, pH 5.5, 15% methyl ether poly(ethylene glycol) 5000, 10% hexanediol, 100 mM CaCl2, and 1 mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.45 Å
R-free 0.181
|
|
2Q1N
Actin Dimer Cross-linked Between Residues 41 and 374
Deposited 2007-05-25
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 2
PDB declaration: dimeric
|
Chain A
3–377（375 aa）
|
Not recorded
|
CA CALCIUM ION × 4
ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2
LAR LATRUNCULIN A × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；298 K；30% MPD, 0.1M sodium acetate, 0.02 M calcium chloride, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.70 Å
R-free 0.277
|
|
2Q1N
Actin Dimer Cross-linked Between Residues 41 and 374
Deposited 2007-05-25
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Protein homooligomer
Homooligomer；Protein × 2
PDB declaration: dimeric
|
Chain B
3–377（375 aa）
|
Not recorded
|
CA CALCIUM ION × 4
ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2
LAR LATRUNCULIN A × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；298 K；30% MPD, 0.1M sodium acetate, 0.02 M calcium chloride, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.70 Å
R-free 0.277
|
|
2Q31
Actin Dimer Cross-linked Between Residues 41 and 374 and proteolytically cleaved by subtilisin between residues 47 and 48.
Deposited 2007-05-29
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 2
PDB declaration: dimeric
|
Chain A
3–377（375 aa）
|
Not recorded
|
CA CALCIUM ION × 2
ATP ADENOSINE-5'-TRIPHOSPHATE × 2
LAR LATRUNCULIN A × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；298 K；30% MPD, 0.1 M sodium acetate, 0.01M calcium chloride, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.70 Å
R-free 0.288
|
|
2Q31
Actin Dimer Cross-linked Between Residues 41 and 374 and proteolytically cleaved by subtilisin between residues 47 and 48.
Deposited 2007-05-29
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Protein homooligomer
Homooligomer；Protein × 2
PDB declaration: dimeric
|
Chain B
3–377（375 aa）
|
Not recorded
|
CA CALCIUM ION × 2
ATP ADENOSINE-5'-TRIPHOSPHATE × 2
LAR LATRUNCULIN A × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；298 K；30% MPD, 0.1 M sodium acetate, 0.01M calcium chloride, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.70 Å
R-free 0.288
|
|
2Q36
Actin Dimer Cross-linked between Residues 191 and 374 and complexed with Kabiramide C
Deposited 2007-05-29
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 2
PDB declaration: dimeric
|
Chain A
3–377（375 aa）
|
Not recorded
|
CA CALCIUM ION × 2
SO4 SULFATE ION × 4
ATP ADENOSINE-5'-TRIPHOSPHATE × 2
KAB KABIRAMIDE C × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；298 K；30% PEG4000, 0.1 M Tris, 0.2 M lithium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.50 Å
R-free 0.223
|
|
2Q97
Complex of mammalian actin with toxofilin from toxoplasma gondii
Deposited 2007-06-12
|
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
CA CALCIUM ION × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 4.6；277 K；8% PEG 4000, 10% GLYCEROL, 0.1M SODIUM ACETATE, pH 4.6, VAPOR DIFFUSION, temperature 277K
|
Resolution 2.50 Å
R-free 0.284
|
|
2Q97
Complex of mammalian actin with toxofilin from toxoplasma gondii
Deposited 2007-06-12
|
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Protein heterocomplex
Heteromer；Protein × 4
PDB declaration: tetrameric
|
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
CA CALCIUM ION × 2
ATP ADENOSINE-5'-TRIPHOSPHATE × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 4.6；277 K；8% PEG 4000, 10% GLYCEROL, 0.1M SODIUM ACETATE, pH 4.6, VAPOR DIFFUSION, temperature 277K
|
Resolution 2.50 Å
R-free 0.284
|
|
2V51
Structure of MAL-RPEL1 complexed to actin
Deposited 2008-10-01
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 4
PDB declaration: tetrameric
|
Chain B
1–377（377 aa）
Chain D
1–377（377 aa）
|
Not recorded
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 2
CA CALCIUM ION × 2
LAB LATRUNCULIN B × 2
PEG DI(HYDROXYETHYL)ETHER × 2
SCN THIOCYANATE ION × 4
|
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.5；pH 6.5
|
Resolution 2.35 Å
R-free 0.248
|
|
2V52
Structure of MAL-RPEL2 complexed to G-actin
Deposited 2008-10-01
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain B
1–377（377 aa）
|
Not recorded
|
LAB LATRUNCULIN B × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 1
GOL GLYCEROL × 2
|
X-RAY DIFFRACTION
mmCIF provides none of the parsed conditions
|
Resolution 1.45 Å
R-free 0.188
|
|
2VCP
Crystal structure of N-Wasp VC domain in complex with skeletal actin
Deposited 2007-09-26
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain A
3–377（375 aa）
Fragment：RESIDUES 3-377
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
CA CALCIUM ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7；277 K；VAPOR DIFFUSION METHOD (4 C) PROTEIN SOLUTION: 0.18MM ACTIN, 0.36MM N-WASP PEPTIDE, 5MM TRIS.HCL PH7.0, ATP 0.2MM, CACL2 0.02MM, TCEP 20MM, NAN3 0.01%. RESERVOIR: 10.2% (V/V) TACSIMATE, 13.2%(W/V)PEG 8000, 100MM HEPES PH7.0
|
Resolution 3.20 Å
R-free 0.331
|
|
2VCP
Crystal structure of N-Wasp VC domain in complex with skeletal actin
Deposited 2007-09-26
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain B
3–377（375 aa）
Fragment：RESIDUES 3-377
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
CA CALCIUM ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7；277 K；VAPOR DIFFUSION METHOD (4 C) PROTEIN SOLUTION: 0.18MM ACTIN, 0.36MM N-WASP PEPTIDE, 5MM TRIS.HCL PH7.0, ATP 0.2MM, CACL2 0.02MM, TCEP 20MM, NAN3 0.01%. RESERVOIR: 10.2% (V/V) TACSIMATE, 13.2%(W/V)PEG 8000, 100MM HEPES PH7.0
|
Resolution 3.20 Å
R-free 0.331
|
|
2VYP
Rabbit-muscle G-actin in complex with myxobacterial rhizopodin
Deposited 2008-07-25
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain A
1–377（377 aa）
|
Not recorded
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
CA CALCIUM ION × 1
RH9 RHIZOPODIN × 1
HEZ HEXANE-1,6-DIOL × 3
|
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.6；100 MM MES PH 6.6 14% (W/W) PEG1500 12% (W/W) 1,6-HEXANEDIOL 100 MM CACL2 1 MM DTT 10 MM BETAINE-HYDROCHLORIDE
|
Resolution 2.35 Å
R-free 0.241
|
|
2VYP
Rabbit-muscle G-actin in complex with myxobacterial rhizopodin
Deposited 2008-07-25
|
Different construct
Different oligomeric state
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain B
1–377（377 aa）
|
Not recorded
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
CA CALCIUM ION × 3
|
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.6；100 MM MES PH 6.6 14% (W/W) PEG1500 12% (W/W) 1,6-HEXANEDIOL 100 MM CACL2 1 MM DTT 10 MM BETAINE-HYDROCHLORIDE
|
Resolution 2.35 Å
R-free 0.241
|
|
2W49
ISOMETRICALLY CONTRACTING INSECT ASYNCHRONOUS FLIGHT MUSCLE
Deposited 2008-11-24
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 36
PDB declaration: 36-meric
|
Chain D
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain E
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain F
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain G
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain H
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain I
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain J
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain K
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain L
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain M
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain N
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain O
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain P
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain Q
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain R
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain S
3–374（372 aa）
Fragment：RESIDUES 3-374
|
Not recorded
|
CA CALCIUM ION × 16
|
ELECTRON MICROSCOPY
cryo-EM buffer
20 MM MOPS BUFFER, 5 MM NAN3, AND MGCL2, ATP, CACL2, AND EGTA IN VARYING MILLIMOLAR CONCENTRATIONS；20 MM MOPS BUFFER, 5 MM NAN3, AND MGCL2, ATP, CACL2, AND EGTA IN VARYING MILLIMOLAR CONCENTRATIONS
cryo-EM vitrification conditions
Cryogen HELIUM；SMASH AGAINST A LIQUID HELIUM COOLED GOLD COATED COPPER MIRROR
|
Resolution 35.00 Å
|
|
2W4U
Isometrically contracting insect asynchronous flight muscle quick frozen after a length step
Deposited 2008-12-02
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 36
PDB declaration: 36-meric
|
Chain D
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain E
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain F
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain G
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain H
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain I
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain J
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain K
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain L
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain M
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain N
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain O
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain P
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain Q
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain R
3–374（372 aa）
Fragment：RESIDUES 3-374
Chain S
3–374（372 aa）
Fragment：RESIDUES 3-374
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM buffer
20 MM MOPS BUFFER, 5 MM NAN3, AND MGCL2, ATP, CACL2, AND EGTA IN VARYING MILLIMOLAR CONCENTRATIONS；20 MM MOPS BUFFER, 5 MM NAN3, AND MGCL2, ATP, CACL2, AND EGTA IN VARYING MILLIMOLAR CONCENTRATIONS
cryo-EM vitrification conditions
Cryogen HELIUM；SMASH AGAINST A LIQUID HELIUM COOLED GOLD COATED COPPER MIRROR
|
Resolution 35.00 Å
|
|
2Y83
Actin filament pointed end
Deposited 2011-02-02
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 6
PDB declaration: hexameric
|
Chain O
3–377（375 aa）
Chain P
3–377（375 aa）
Chain Q
3–377（375 aa）
Chain R
3–377（375 aa）
Chain S
3–377（375 aa）
Chain T
3–377（375 aa）
|
Not recorded
|
ADP ADENOSINE-5'-DIPHOSPHATE × 6
CA CALCIUM ION × 6
|
ELECTRON MICROSCOPY
cryo-EM buffer
50 MM NACL, 10 MM SODIUM PHOSPHATE BUFFER PH 7.4 3 MM MGCL2, 0.005% (W/V) NAN3, 0.7 MM DTT.；pH 7.4；50 MM NACL, 10 MM SODIUM PHOSPHATE BUFFER PH 7.4 3 MM MGCL2, 0.005% (W/V) NAN3, 0.7 MM DTT.
cryo-EM vitrification conditions
Cryogen ETHANE；VITRIFICATION 1 - CRYOGEN- ETHANE, HUMIDITY- 90%, TEMPERATURE- 4 DEGREES CELSIUS. METHOD- BLOT FOR 3 SECONDS BEFORE PLUNGING.
|
Resolution 22.90 Å
|
|
2YJE
Oligomeric assembly of actin bound to MRTF-A
Deposited 2011-05-19
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 4
PDB declaration: tetrameric
|
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
|
Not recorded
|
LAB LATRUNCULIN B × 3
ATP ADENOSINE-5'-TRIPHOSPHATE × 3
MG MAGNESIUM ION × 3
|
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8.25；0.1 M BTP PH 8.25, 20.5% PEG 3350, 0.2 M SODIUM NITRATE.
|
Resolution 3.10 Å
R-free 0.280
|
|
2YJF
Oligomeric assembly of actin bound to MRTF-A
Deposited 2011-05-19
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 4
PDB declaration: tetrameric
|
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
|
Not recorded
|
LAB LATRUNCULIN B × 2
ATP ADENOSINE-5'-TRIPHOSPHATE × 3
MG MAGNESIUM ION × 3
|
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5.3；pH 5.3
|
Resolution 3.50 Å
R-free 0.272
|
|
2YJF
Oligomeric assembly of actin bound to MRTF-A
Deposited 2011-05-19
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain D
1–377（377 aa）
|
Not recorded
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5.3；pH 5.3
|
Resolution 3.50 Å
R-free 0.272
|
|
2YJF
Oligomeric assembly of actin bound to MRTF-A
Deposited 2011-05-19
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 3
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain E
1–377（377 aa）
|
Not recorded
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5.3；pH 5.3
|
Resolution 3.50 Å
R-free 0.272
|
|
2ZWH
Model for the F-actin structure
Deposited 2008-12-05
|
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 1
CA CALCIUM ION × 1
|
FIBER DIFFRACTION
mmCIF provides none of the parsed conditions
|
Resolution 3.30 Å
|
|
3B5U
Actin filament model from extended form of acromsomal bundle in the Limulus sperm
Deposited 2007-10-26
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 14
PDB declaration: tetradecameric
|
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
Chain H
1–377（377 aa）
Chain I
1–377（377 aa）
Chain J
1–377（377 aa）
Chain K
1–377（377 aa）
Chain L
1–377（377 aa）
Chain M
1–377（377 aa）
Chain N
1–377（377 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON CRYSTALLOGRAPHY
cryo-EM buffer
pH 7.4；see J Mol Biol, 221, 711-725 (1991)
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 9.50 Å
|
|
3BUZ
Crystal structure of ia-bTAD-actin complex
Deposited 2008-01-04
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain B
3–377（375 aa）
|
Not recorded
|
TAD BETA-METHYLENE-THIAZOLE-4-CARBOXYAMIDE-ADENINE DINUCLEOTIDE × 1
CA CALCIUM ION × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
LAR LATRUNCULIN A × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；20% PEG1000, 0.1M MES(pH6.5), VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.81 Å
R-free 0.298
|
|
3CJB
Actin dimer cross-linked by V. cholerae MARTX toxin and complexed with Gelsolin-segment 1
Deposited 2008-03-12
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain A
1–377（377 aa）
|
Not recorded
|
CA CALCIUM ION × 2
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；10% Peg MME 5000, 5% Tacsimate, 0.1 M Hepes, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.21 Å
R-free 0.269
|
|
3CJC
Actin dimer cross-linked by V. cholerae MARTX toxin and complexed with DNase I and Gelsolin-segment 1
Deposited 2008-03-12
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Other combination
Heteromer；Protein × 3
PDB declaration: trimeric
|
Chain A
1–377（377 aa）
|
Not recorded
|
CA CALCIUM ION × 2
SO4 SULFATE ION × 6
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；1.5 M (NH4)SO4, 0.1 M Bis-Tris, 0.1 M NaCl, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.90 Å
R-free 0.278
|
|
3DAW
Structure of the actin-depolymerizing factor homology domain in complex with actin
Deposited 2008-05-30
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain A
1–377（377 aa）
|
Not recorded
|
CA CALCIUM ION × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；10mM Tris pH 7.5, 50mM NaCl, 0.2mM ATP, 0.2mM DTT, 0.2mM CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.55 Å
R-free 0.279
|
|
3FFK
Crystal structure of human Gelsolin domains G1-G3 bound to Actin
Deposited 2008-12-03
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain B
1–377（377 aa）
|
Not recorded
|
CA CALCIUM ION × 5
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；297 K；9% PEG 4000, 100 mM Sodium Acetate, 100 mM Calcium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 297K
|
Resolution 3.00 Å
R-free 0.273
|
|
3FFK
Crystal structure of human Gelsolin domains G1-G3 bound to Actin
Deposited 2008-12-03
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain E
1–377（377 aa）
|
Not recorded
|
CA CALCIUM ION × 5
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；297 K；9% PEG 4000, 100 mM Sodium Acetate, 100 mM Calcium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 297K
|
Resolution 3.00 Å
R-free 0.273
|
|
3G37
Cryo-EM structure of actin filament in the presence of phosphate
Deposited 2009-02-02
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 12
PDB declaration: dodecameric
|
Chain O
3–377（375 aa）
Chain P
3–377（375 aa）
Chain Q
3–377（375 aa）
Chain R
3–377（375 aa）
Chain S
3–377（375 aa）
Chain T
3–377（375 aa）
Chain U
3–377（375 aa）
Chain V
3–377（375 aa）
Chain W
3–377（375 aa）
Chain X
3–377（375 aa）
Chain Y
3–377（375 aa）
Chain Z
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 12
PO4 PHOSPHATE ION × 36
MG MAGNESIUM ION × 72
|
ELECTRON MICROSCOPY
cryo-EM buffer
phosphate buffer；pH 7.4；phosphate buffer
cryo-EM vitrification conditions
77 K；Cryogen ETHANE
|
Resolution 6.00 Å
|
|
3HBT
The structure of native G-actin
Deposited 2009-05-05
|
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
SO4 SULFATE ION × 1
CA CALCIUM ION × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；295 K；2M ammonium sulfate, 100mM Tris-HCl, pH8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.70 Å
R-free 0.257
|
|
3J4K
Cryo-EM structures of the actin:tropomyosin filament reveal the mechanism for the transition from C- to M-state
Deposited 2013-08-26
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 7
PDB declaration: heptameric
|
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Not recorded
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
70 mM NaCl, 3 mM MgCl2, 0.2 mM EGTA, 5 mM NaH2PO4, 5 mM PIPES buffer；pH 7.5；70 mM NaCl, 3 mM MgCl2, 0.2 mM EGTA, 5 mM NaH2PO4, 5 mM PIPES buffer
cryo-EM vitrification conditions
3 second blot；Cryogen ETHANE；3 second blot before plunging into liquid ethane (FEI Vitrobot Mark IV)
|
Resolution 8.00 Å
|
|
3J8A
Structure of the F-actin-tropomyosin complex
Deposited 2014-10-08
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 7
PDB declaration: heptameric
|
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
MG MAGNESIUM ION × 5
ADP ADENOSINE-5'-DIPHOSPHATE × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
5 mM Tris-HCl, pH 7.5, 1 mM DTT, 100 mM KCl, 2 mM MgCl2；pH 7.5；5 mM Tris-HCl, pH 7.5, 1 mM DTT, 100 mM KCl, 2 mM MgCl2
cryo-EM vitrification conditions
Sample was applied to grid, incubated for 10 seconds, and manually blotted for 3 seconds from the backside with filter paper.；106 K；Cryogen ETHANE；Sample was applied to grid, incubated for 10 seconds, and manually blotted for 3 seconds from the backside with filter paper before plunging into liquid ethane (GATAN CRYOPLUNGE 3)
|
Resolution 3.70 Å
R-free 0.271
|
|
3J8I
Near-Atomic Resolution for One State of F-Actin
Deposited 2014-11-06
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 5
PDB declaration: pentameric
|
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
Chain H
1–377（377 aa）
|
Not recorded
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
MG MAGNESIUM ION × 5
|
ELECTRON MICROSCOPY
cryo-EM vitrification conditions
Cryogen ETHANE；Plunged into liquid ethane (FEI VITROBOT MARK IV)
|
Resolution 4.70 Å
|
|
3J8J
Tilted state of actin, T1
Deposited 2014-11-07
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 11
PDB declaration: undecameric
|
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
Chain H
1–377（377 aa）
Chain I
1–377（377 aa）
Chain J
1–377（377 aa）
Chain K
1–377（377 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM vitrification conditions
Cryogen ETHANE；Plunged into liquid ethane
|
Resolution 12.00 Å
|
|
3J8K
Tilted state of actin, T2
Deposited 2014-11-07
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 10
PDB declaration: decameric
|
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
Chain H
1–377（377 aa）
Chain I
1–377（377 aa）
Chain J
1–377（377 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 12.00 Å
|
|
3JBI
MDFF model of the vinculin tail domain bound to F-actin
Deposited 2015-09-02
|
Different construct
Different mutation/modification
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 3
PDB declaration: trimeric
|
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
|
Not recorded
|
MG MAGNESIUM ION × 2
ADP ADENOSINE-5'-DIPHOSPHATE × 2
|
ELECTRON MICROSCOPY
cryo-EM buffer
50 mM KCl, 1 mM MgCl2, 1 mM EGTA, 10 mM imidazole；pH 7；50 mM KCl, 1 mM MgCl2, 1 mM EGTA, 10 mM imidazole
cryo-EM vitrification conditions
3 microliters of 0.3 micromolar actin was applied to the grid and incubated for 60 seconds at 25 degrees C. 3 microliters of 10 micromolar Vt was then applied and incubated for 60 seconds. 3 microliters of solution was removed, then an additional 3 microliters of Vt applied. After 60 seconds, 3 microliters of solution was removed, then the grid was blotted for 2 seconds before plunging.；Cryogen ETHANE；3 microliters of 0.3 micromolar actin was applied to the grid and incubated for 60 seconds at 25 degrees C. 3 microliters of 10 micromolar Vt was then applied and incubated for 60 seconds. 3 microliters of solution was removed, then an additional 3 microliters of Vt applied. After 60 seconds, 3 microliters of solution was removed, then the grid was blotted for 2 seconds before plunging into liquid ethane (LEICA EM GP).
|
Resolution 8.50 Å
|
|
3JBJ
Cryo-EM reconstruction of F-actin
Deposited 2015-09-03
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 2
PDB declaration: dimeric
|
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
|
Not recorded
|
MG MAGNESIUM ION × 2
ADP ADENOSINE-5'-DIPHOSPHATE × 2
|
ELECTRON MICROSCOPY
cryo-EM buffer
50 mM KCl, 1 mM MgCl2, 1 mM EGTA, 10 mM imidazole；pH 7；50 mM KCl, 1 mM MgCl2, 1 mM EGTA, 10 mM imidazole
cryo-EM vitrification conditions
3 microliters of 0.3 micromolar actin was applied to the grid and incubated for 60 seconds at 25 degrees C. The grid was blotted for 2 seconds before plunging.；Cryogen ETHANE；3 microliters of 0.3 micromolar actin was applied to the grid and incubated for 60 seconds at 25 degrees C. The grid was blotted for 2 seconds before plunging into liquid ethane (LEICA EM GP).
|
Resolution 7.60 Å
|
|
3JBK
Cryo-EM reconstruction of the metavinculin-actin interface
Deposited 2015-09-03
|
Different construct
Different mutation/modification
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 3
PDB declaration: trimeric
|
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
|
Not recorded
|
MG MAGNESIUM ION × 2
ADP ADENOSINE-5'-DIPHOSPHATE × 2
|
ELECTRON MICROSCOPY
cryo-EM buffer
50 mM KCl, 1 mM MgCl2, 1 mM EGTA, 10 mM imidazole；pH 7；50 mM KCl, 1 mM MgCl2, 1 mM EGTA, 10 mM imidazole
cryo-EM vitrification conditions
3 microliters of 0.3 micromolar actin was applied to the grid and incubated for 60 seconds at 25 degrees C. 3 microliters of 10 micromolar MVt was then applied and incubated for 60 seconds. 3 microliters of solution was removed, then an additional 3 microliters of MVt applied. After 60 seconds, 3 microliters of solution was removed, then the grid was blotted for 2 seconds before plunging.；Cryogen ETHANE；3 microliters of 0.3 micromolar actin was applied to the grid and incubated for 60 seconds at 25 degrees C. 3 microliters of 10 micromolar MVt was then applied and incubated for 60 seconds. 3 microliters of solution was removed, then an additional 3 microliters of MVt applied. After 60 seconds, 3 microliters of solution was removed, then the grid was blotted for 2 seconds before plunging into liquid ethane (LEICA EM GP).
|
Resolution 8.20 Å
|
|
3M1F
Crosslinked complex of actin with first W domain of Vibrio parahaemolyticus VopL
Deposited 2010-03-04
|
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
CA CALCIUM ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.1；298 K；0.2 N Lithium Nitrate, 20% polyethylene glycol 3350, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
Resolution 2.89 Å
R-free 0.265
|
|
3M3N
Structure of a Longitudinal Actin Dimer Assembled by Tandem W Domains
Deposited 2010-03-09
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Insufficient information
Heteromer；Protein × 3
PDB declaration: trimeric
|
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 2
CA CALCIUM ION × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 10；300 K；100 mM CAPS pH 10.0, and 24% PEG 3350, 100 mM RbCl, VAPOR DIFFUSION, HANGING DROP, temperature 300.0K
|
Resolution 7.00 Å
|
|
3M6G
Crystal structure of actin in complex with lobophorolide
Deposited 2010-03-15
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 2
PDB declaration: dimeric
|
Chain A
3–373（371 aa）
Fragment：UNP residues 3-373
Chain B
3–373（371 aa）
Fragment：UNP residues 3-373
|
Not recorded
|
MG MAGNESIUM ION × 2
ATP ADENOSINE-5'-TRIPHOSPHATE × 2
LO3 (1S,3S,4S,5S,7R,8S,9R,12E,14E,16R,17R,19R)-16-hydroxy-9-{(1S,2S,3S)-2-hydroxy-5-[(2S,4R,6S)-4-methoxy-6-methyltetrahydro-2H-pyran-2-yl]-1,3-dimethylpentyl}-3,5,7,17-tetramethoxy-8,14-dimethyl-11H-spiro[10,23-dioxabicyclo[17.3.1]tricosa-12,14,20-triene-4,2'-oxiran]-11-one × 2
CA CALCIUM ION × 4
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；0.1M MES pH 6.0, 6% methyl
ether poly(ethylene glycol) 5000, 0.1M CaCl2, 1mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.00 Å
R-free 0.227
|
|
3M6G
Crystal structure of actin in complex with lobophorolide
Deposited 2010-03-15
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain A
3–373（371 aa）
Fragment：UNP residues 3-373
|
Not recorded
|
MG MAGNESIUM ION × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
LO3 (1S,3S,4S,5S,7R,8S,9R,12E,14E,16R,17R,19R)-16-hydroxy-9-{(1S,2S,3S)-2-hydroxy-5-[(2S,4R,6S)-4-methoxy-6-methyltetrahydro-2H-pyran-2-yl]-1,3-dimethylpentyl}-3,5,7,17-tetramethoxy-8,14-dimethyl-11H-spiro[10,23-dioxabicyclo[17.3.1]tricosa-12,14,20-triene-4,2'-oxiran]-11-one × 1
CA CALCIUM ION × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；0.1M MES pH 6.0, 6% methyl
ether poly(ethylene glycol) 5000, 0.1M CaCl2, 1mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.00 Å
R-free 0.227
|
|
3M6G
Crystal structure of actin in complex with lobophorolide
Deposited 2010-03-15
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 3
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain B
3–373（371 aa）
Fragment：UNP residues 3-373
|
Not recorded
|
MG MAGNESIUM ION × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
LO3 (1S,3S,4S,5S,7R,8S,9R,12E,14E,16R,17R,19R)-16-hydroxy-9-{(1S,2S,3S)-2-hydroxy-5-[(2S,4R,6S)-4-methoxy-6-methyltetrahydro-2H-pyran-2-yl]-1,3-dimethylpentyl}-3,5,7,17-tetramethoxy-8,14-dimethyl-11H-spiro[10,23-dioxabicyclo[17.3.1]tricosa-12,14,20-triene-4,2'-oxiran]-11-one × 1
CA CALCIUM ION × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；0.1M MES pH 6.0, 6% methyl
ether poly(ethylene glycol) 5000, 0.1M CaCl2, 1mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.00 Å
R-free 0.227
|
|
3MFP
Atomic model of F-actin based on a 6.6 angstrom resolution cryoEM map
Deposited 2010-04-03
|
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 5
PDB declaration: pentameric
|
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.60 Å
|
|
3MFP
Atomic model of F-actin based on a 6.6 angstrom resolution cryoEM map
Deposited 2010-04-03
|
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.60 Å
|
|
3MFP
Atomic model of F-actin based on a 6.6 angstrom resolution cryoEM map
Deposited 2010-04-03
|
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 3
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.60 Å
|
|
3MN5
Structures of actin-bound WH2 domains of Spire and the implication for filament nucleation
Deposited 2010-04-21
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain A
3–377（375 aa）
|
Not recorded
|
CA CALCIUM ION × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
LAB LATRUNCULIN B × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.9；277 K；0.2 Magnesium formate pH 5.9
20% PEG 3350
, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 1.50 Å
R-free 0.216
|
|
3SJH
Crystal Structure of a chimera containing the N-terminal domain (residues 8-29) of drosophila Ciboulot and the C-terminal domain (residues 18-44) of bovine Thymosin-beta4, bound to G-actin-ATP-Latrunculin A
Deposited 2011-06-21
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Insufficient information
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain A
3–377（375 aa）
Fragment：UNP residues 3-377
|
Not recorded
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 1
LAR LATRUNCULIN A × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
hanging drop；pH 6.5；298 K；22% PEG3350, 0.2M MgAcetate pH6.5, 0.45M Guanidine HCl, 1% Dioxane, hanging drop, temperature 298K
|
Resolution 1.75 Å
R-free 0.199
|
|
3TPQ
Crystal structure of wild-type MAL RPEL domain in complex with five G-actins
Deposited 2011-09-08
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 6
PDB declaration: hexameric
|
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Not recorded
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 5
CA CALCIUM ION × 5
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 5.3；293 K；50mM citrate, 0.1M ammonium sulfate, 0.1mM CaCl2, 0.1mM ATP, 10% PEG8000, pH 5.3, VAPOR DIFFUSION, temperature 293K
|
Resolution 3.45 Å
R-free 0.273
|
|
3TU5
Actin complex with Gelsolin Segment 1 fused to Cobl segment
Deposited 2011-09-15
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Insufficient information
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain A
1–377（377 aa）
|
Not recorded
|
CA CALCIUM ION × 2
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；298 K；10% (w/v) polyethyleneglycol 20000, 2% (v/v) dioxane, and 0.1M bicine, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.00 Å
R-free 0.204
|
|
3U8X
Crystal Structure of a chimera containing the N-terminal domain (residues 8-29) of drosophila Ciboulot and the C-terminal domain (residues 18-44) of bovine Thymosin-beta4, bound to G-actin-ATP
Deposited 2011-10-17
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Insufficient information
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain A
3–377（375 aa）
|
Not recorded
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
hanging drop；pH 6.5；298 K；20% PEG3350, 0.2M MgAcetate pH6.5, 0.45M Guanidine HCl, 1% Dioxane, hanging drop, temperature 298K
|
Resolution 2.00 Å
R-free 0.238
|
|
3U8X
Crystal Structure of a chimera containing the N-terminal domain (residues 8-29) of drosophila Ciboulot and the C-terminal domain (residues 18-44) of bovine Thymosin-beta4, bound to G-actin-ATP
Deposited 2011-10-17
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Insufficient information
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain C
3–377（375 aa）
|
Not recorded
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
hanging drop；pH 6.5；298 K；20% PEG3350, 0.2M MgAcetate pH6.5, 0.45M Guanidine HCl, 1% Dioxane, hanging drop, temperature 298K
|
Resolution 2.00 Å
R-free 0.238
|
|
3U9Z
Crystal structure between actin and a protein construct containing the first beta-thymosin domain of drosophila ciboulot (residues 2-58) with the three mutations N26D/Q27K/D28S
Deposited 2011-10-20
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain A
3–377（375 aa）
|
Not recorded
|
ADP ADENOSINE-5'-DIPHOSPHATE × 1
MG MAGNESIUM ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
hanging drop；pH 6.5；298 K；18% PEG3350, 0.05M NaAcetate pH4.7, 0.1M MgAcetate pH6.5, 0.32M Guanidine HCl, 0.8% Dioxane, hanging drop, temperature 298K
|
Resolution 2.09 Å
R-free 0.227
|
|
3UE5
ECP-cleaved Actin in complex with Spir domain D
Deposited 2011-10-28
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 4
PDB declaration: tetrameric
|
Chain A
3–377（375 aa）
|
Not recorded
|
CA CALCIUM ION × 2
ATP ADENOSINE-5'-TRIPHOSPHATE × 2
CL CHLORIDE ION × 4
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；298 K；Tris pH 8.5, magnesium chloride, PEG-8000, vapor diffusion, hanging drop, temperature 298K
|
Resolution 2.76 Å
R-free 0.240
|
|
3UE5
ECP-cleaved Actin in complex with Spir domain D
Deposited 2011-10-28
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Protein heterocomplex
Heteromer；Protein × 4
PDB declaration: tetrameric
|
Chain A
3–377（375 aa）
|
Not recorded
|
CA CALCIUM ION × 2
ATP ADENOSINE-5'-TRIPHOSPHATE × 2
CL CHLORIDE ION × 4
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；298 K；Tris pH 8.5, magnesium chloride, PEG-8000, vapor diffusion, hanging drop, temperature 298K
|
Resolution 2.76 Å
R-free 0.240
|
|
3UE5
ECP-cleaved Actin in complex with Spir domain D
Deposited 2011-10-28
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 3
Protein heterocomplex
Heteromer；Protein × 4
PDB declaration: tetrameric
|
Chain A
3–377（375 aa）
|
Not recorded
|
CA CALCIUM ION × 2
ATP ADENOSINE-5'-TRIPHOSPHATE × 2
CL CHLORIDE ION × 4
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；298 K；Tris pH 8.5, magnesium chloride, PEG-8000, vapor diffusion, hanging drop, temperature 298K
|
Resolution 2.76 Å
R-free 0.240
|
|
4A7F
Structure of the Actin-Tropomyosin-Myosin Complex (rigor ATM 3)
Deposited 2011-11-14
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 10
PDB declaration: decameric
|
Chain A
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
Chain F
3–377（375 aa）
Chain I
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
CA CALCIUM ION × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
5 MM HEPES-OH, 100 MM KCL, 2 MM MGCL2, 50 MM GLUTAMINE, 50 MM ARGININE；pH 7.2；5 MM HEPES-OH, 100 MM KCL, 2 MM MGCL2, 50 MM GLUTAMINE, 50 MM ARGININE
cryo-EM vitrification conditions
Cryogen ETHANE；LIQUID ETHANE
|
Resolution 7.70 Å
|
|
4A7H
Structure of the Actin-Tropomyosin-Myosin Complex (rigor ATM 2)
Deposited 2011-11-14
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 10
PDB declaration: decameric
|
Chain A
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
Chain F
3–377（375 aa）
Chain G
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
CA CALCIUM ION × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
5MM TRIS, 100MM KCL, 2MM MGCL2, 50MM GLUTAMINE, 50MM ARGININ；pH 7.2；5MM TRIS, 100MM KCL, 2MM MGCL2, 50MM GLUTAMINE, 50MM ARGININ
cryo-EM vitrification conditions
Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 90, TEMPERATURE- 101, INSTRUMENT- GATAN CRYOPLUNGE 3, METHOD- MANUAL BLOTTING FOR APPROXIMATELY 15 SECONDS,
|
Resolution 7.80 Å
|
|
4A7L
Structure of the Actin-Tropomyosin-Myosin Complex (rigor ATM 1)
Deposited 2011-11-14
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 10
PDB declaration: decameric
|
Chain A
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
Chain F
3–377（375 aa）
Chain I
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
CA CALCIUM ION × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
5MM TRIS, 100MM KCL, 2MM MGCL2, 50MM GLUTAMINE, 50MM ARGININ；pH 7.2；5MM TRIS, 100MM KCL, 2MM MGCL2, 50MM GLUTAMINE, 50MM ARGININ
cryo-EM vitrification conditions
Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 90, TEMPERATURE- 101, INSTRUMENT- GATAN CRYOPLUNGE 3, METHOD- MANUAL BLOTTING FOR APPROXIMATELY 15 SECONDS,
|
Resolution 8.10 Å
|
|
4A7N
Structure of bare F-actin filaments obtained from the same sample as the Actin-Tropomyosin-Myosin Complex
Deposited 2011-11-14
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 5
PDB declaration: pentameric
|
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
CA CALCIUM ION × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
5MM TRIS, 100MM KCL, 2MM MGCL2, 50MM GLUTAMINE, 50MM ARGININE；pH 7.2；5MM TRIS, 100MM KCL, 2MM MGCL2, 50MM GLUTAMINE, 50MM ARGININE
cryo-EM vitrification conditions
Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 90, TEMPERATURE- 101, INSTRUMENT- GATAN CRYOPLUNGE 3, METHOD- MANUAL BLOTTING FOR APPROXIMATELY 15 SECONDS,
|
Resolution 8.90 Å
|
|
4B1V
Structure of the Phactr1 RPEL-N domain bound to G-actin
Deposited 2012-07-12
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain B
2–377（376 aa）
Fragment：RESIDUES 2-377
|
Not recorded
|
EDO 1,2-ETHANEDIOL × 3
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
LAB LATRUNCULIN B × 1
MG MAGNESIUM ION × 1
GOL GLYCEROL × 2
|
X-RAY DIFFRACTION
mmCIF provides none of the parsed conditions
|
Resolution 1.75 Å
R-free 0.221
|
|
4B1V
Structure of the Phactr1 RPEL-N domain bound to G-actin
Deposited 2012-07-12
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain A
2–377（376 aa）
Fragment：RESIDUES 2-377
|
Not recorded
|
EDO 1,2-ETHANEDIOL × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
LAB LATRUNCULIN B × 1
MG MAGNESIUM ION × 1
|
X-RAY DIFFRACTION
mmCIF provides none of the parsed conditions
|
Resolution 1.75 Å
R-free 0.221
|
|
4B1W
Structure of the Phactr1 RPEL-2 domain bound to actin
Deposited 2012-07-12
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain B
2–377（376 aa）
|
Not recorded
|
LAB LATRUNCULIN B × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 1
SO4 SULFATE ION × 1
GOL GLYCEROL × 1
|
X-RAY DIFFRACTION
mmCIF provides none of the parsed conditions
|
Resolution 1.95 Å
R-free 0.213
|
|
4B1X
Structure of the Phactr1 RPEL-2 bound to G-actin
Deposited 2012-07-12
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain B
2–377（376 aa）
|
Not recorded
|
LAB LATRUNCULIN B × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 1
|
X-RAY DIFFRACTION
mmCIF provides none of the parsed conditions
|
Resolution 1.80 Å
R-free 0.213
|
|
4B1Y
Structure of the Phactr1 RPEL-3 bound to G-actin
Deposited 2012-07-12
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain B
2–377（376 aa）
|
Not recorded
|
LAB LATRUNCULIN B × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 1
1PE PENTAETHYLENE GLYCOL × 1
GOL GLYCEROL × 1
PEG DI(HYDROXYETHYL)ETHER × 3
P6G HEXAETHYLENE GLYCOL × 1
|
X-RAY DIFFRACTION
mmCIF provides none of the parsed conditions
|
Resolution 1.29 Å
R-free 0.174
|
|
4B1Z
Structure of the Phactr1 RPEL domain bound to G-actin
Deposited 2012-07-12
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 8
PDB declaration: octameric
|
Chain A
2–377（376 aa）
Chain B
2–377（376 aa）
Chain C
2–377（376 aa）
Chain D
2–377（376 aa）
Chain E
2–377（376 aa）
Chain F
2–377（376 aa）
|
Not recorded
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 6
MG MAGNESIUM ION × 6
GOL GLYCEROL × 8
|
X-RAY DIFFRACTION
mmCIF provides none of the parsed conditions
|
Resolution 3.30 Å
R-free 0.236
|
|
4EAH
Crystal structure of the formin homology 2 domain of FMNL3 bound to actin
Deposited 2012-03-22
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 4
PDB declaration: tetrameric
|
Chain D
1–377（377 aa）
Chain G
1–377（377 aa）
|
Not recorded
|
ACT ACETATE ION × 6
ATP ADENOSINE-5'-TRIPHOSPHATE × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；11% PEG 10000, 0.2 M magnesium acetate, 0.1 M MES, pH 6.5, Silver Bullet 33 (0.20% w/v D-(+)-Maltose monohydrate, 0.20% w/v D-(+)-Melibiose monohydrate, 0.20% w/v D-(+)-Raffinose pentahydrate, 0.20% w/v D-(+)-Trehalose dihydrate, 0.20% w/v Stachyose hydrate, 0.02 M HEPES sodium pH 6.8), Silver Bullet 70 (0.2% w/v Anthrone, 0.2% w/v Benzidine, 0.2% w/v N-(2-Acetamido)-2-aminoethanesulfonic acid, 0.2% w/v Phenylurea, 0.2% w/v -Alanine, 0.02 M HEPES sodium pH 6.8), VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.40 Å
R-free 0.277
|
|
4EAH
Crystal structure of the formin homology 2 domain of FMNL3 bound to actin
Deposited 2012-03-22
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Protein heterocomplex
Heteromer；Protein × 4
PDB declaration: tetrameric
|
Chain F
1–377（377 aa）
Chain H
1–377（377 aa）
|
Not recorded
|
ACT ACETATE ION × 6
ATP ADENOSINE-5'-TRIPHOSPHATE × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；11% PEG 10000, 0.2 M magnesium acetate, 0.1 M MES, pH 6.5, Silver Bullet 33 (0.20% w/v D-(+)-Maltose monohydrate, 0.20% w/v D-(+)-Melibiose monohydrate, 0.20% w/v D-(+)-Raffinose pentahydrate, 0.20% w/v D-(+)-Trehalose dihydrate, 0.20% w/v Stachyose hydrate, 0.02 M HEPES sodium pH 6.8), Silver Bullet 70 (0.2% w/v Anthrone, 0.2% w/v Benzidine, 0.2% w/v N-(2-Acetamido)-2-aminoethanesulfonic acid, 0.2% w/v Phenylurea, 0.2% w/v -Alanine, 0.02 M HEPES sodium pH 6.8), VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.40 Å
R-free 0.277
|
|
4GY2
Crystal structure of apo-Ia-actin complex
Deposited 2012-09-05
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain B
3–377（375 aa）
|
Not recorded
|
PO4 PHOSPHATE ION × 1
CA CALCIUM ION × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
LAR LATRUNCULIN A × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.13 K；18% PEG 1500, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.13K
|
Resolution 2.71 Å
R-free 0.257
|
|
4H03
Crystal structure of NAD+-Ia-actin complex
Deposited 2012-09-07
|
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain B
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1
PO4 PHOSPHATE ION × 1
EDO 1,2-ETHANEDIOL × 48
CA CALCIUM ION × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
LAR LATRUNCULIN A × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.13 K；18% PEG1500, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.13K
|
Resolution 1.75 Å
R-free 0.233
|
|
4H0T
Crystal structure of Ia-ADPR-actin complex
Deposited 2012-09-10
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain B
3–377（375 aa）
|
Not recorded
|
PO4 PHOSPHATE ION × 1
EDO 1,2-ETHANEDIOL × 18
AR6 [(2R,3S,4R,5R)-5-(6-AMINOPURIN-9-YL)-3,4-DIHYDROXY-OXOLAN-2-YL]METHYL[HYDROXY-[[(2R,3S,4R,5S)-3,4,5-TRIHYDROXYOXOLAN-2-YL]METHOXY]PHOSPHORYL] HYDROGEN PHOSPHATE × 1
CA CALCIUM ION × 1
LAR LATRUNCULIN A × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.13 K；18% PEG1500, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.13K
|
Resolution 2.20 Å
R-free 0.240
|
|
4H0V
Crystal structure of NAD+-Ia(E378S)-actin complex
Deposited 2012-09-10
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain B
3–377（375 aa）
|
Not recorded
|
NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1
PO4 PHOSPHATE ION × 1
EDO 1,2-ETHANEDIOL × 39
CA CALCIUM ION × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
LAR LATRUNCULIN A × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.13 K；18% PEG1500, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.13K
|
Resolution 2.03 Å
R-free 0.234
|
|
4H0X
Crystal structure of NAD+-Ia(E380A)-actin complex
Deposited 2012-09-10
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain B
3–377（375 aa）
|
Not recorded
|
NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1
PO4 PHOSPHATE ION × 1
EDO 1,2-ETHANEDIOL × 22
CA CALCIUM ION × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
LAR LATRUNCULIN A × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.13 K；18% PEG1500, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.13K
|
Resolution 2.33 Å
R-free 0.251
|
|
4H0Y
Crystal structure of NAD+-Ia(E380S)-actin complex
Deposited 2012-09-10
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain B
3–377（375 aa）
|
Not recorded
|
NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1
PO4 PHOSPHATE ION × 1
EDO 1,2-ETHANEDIOL × 52
CA CALCIUM ION × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
LAR LATRUNCULIN A × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277.13 K；18% PEG1500, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.13K
|
Resolution 1.94 Å
R-free 0.233
|
|
4K41
Crystal structure of actin in complex with marine macrolide kabiramide C
Deposited 2013-04-11
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain A
3–377（375 aa）
|
Not recorded
|
CA CALCIUM ION × 2
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
KAB KABIRAMIDE C × 1
MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；298 K；0.1 M MES pH 5.5, 0.1 M CaCl2, 12% 1,6-Hexanediol and 17% Polyethylene glycol 1500, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.40 Å
R-free 0.195
|
|
4K42
Crystal structure of actin in complex with synthetic AplC tail analogue SF01 [(3R,4S,5R,6S,10R,11R,12R)-11-(acetyloxy)-1-(benzyloxy)-14-[formyl(methyl)amino]-5-hydroxy-4,6,10,12-tetramethyl-9-oxotetradecan-3-yl propanoate]
Deposited 2013-04-11
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain A
3–377（375 aa）
|
Not recorded
|
CA CALCIUM ION × 1
ADP ADENOSINE-5'-DIPHOSPHATE × 1
NWM (3R,4S,5R,6S,10R,11R,12R)-11-(acetyloxy)-1-(benzyloxy)-14-[formyl(methyl)amino]-5-hydroxy-4,6,10,12-tetramethyl-9-oxotetradecan-3-yl propanoate × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；298 K；0.1 M BIS-TRIS pH 5.5, 25% Polyethylene glycol 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.90 Å
R-free 0.271
|
|
4K42
Crystal structure of actin in complex with synthetic AplC tail analogue SF01 [(3R,4S,5R,6S,10R,11R,12R)-11-(acetyloxy)-1-(benzyloxy)-14-[formyl(methyl)amino]-5-hydroxy-4,6,10,12-tetramethyl-9-oxotetradecan-3-yl propanoate]
Deposited 2013-04-11
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain B
3–377（375 aa）
|
Not recorded
|
CA CALCIUM ION × 1
ADP ADENOSINE-5'-DIPHOSPHATE × 1
NWM (3R,4S,5R,6S,10R,11R,12R)-11-(acetyloxy)-1-(benzyloxy)-14-[formyl(methyl)amino]-5-hydroxy-4,6,10,12-tetramethyl-9-oxotetradecan-3-yl propanoate × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；298 K；0.1 M BIS-TRIS pH 5.5, 25% Polyethylene glycol 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.90 Å
R-free 0.271
|
|
4K42
Crystal structure of actin in complex with synthetic AplC tail analogue SF01 [(3R,4S,5R,6S,10R,11R,12R)-11-(acetyloxy)-1-(benzyloxy)-14-[formyl(methyl)amino]-5-hydroxy-4,6,10,12-tetramethyl-9-oxotetradecan-3-yl propanoate]
Deposited 2013-04-11
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 3
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain C
3–377（375 aa）
|
Not recorded
|
CA CALCIUM ION × 1
ADP ADENOSINE-5'-DIPHOSPHATE × 1
NWM (3R,4S,5R,6S,10R,11R,12R)-11-(acetyloxy)-1-(benzyloxy)-14-[formyl(methyl)amino]-5-hydroxy-4,6,10,12-tetramethyl-9-oxotetradecan-3-yl propanoate × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；298 K；0.1 M BIS-TRIS pH 5.5, 25% Polyethylene glycol 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.90 Å
R-free 0.271
|
|
4K42
Crystal structure of actin in complex with synthetic AplC tail analogue SF01 [(3R,4S,5R,6S,10R,11R,12R)-11-(acetyloxy)-1-(benzyloxy)-14-[formyl(methyl)amino]-5-hydroxy-4,6,10,12-tetramethyl-9-oxotetradecan-3-yl propanoate]
Deposited 2013-04-11
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 4
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain D
3–377（375 aa）
|
Not recorded
|
CA CALCIUM ION × 1
ADP ADENOSINE-5'-DIPHOSPHATE × 1
NWM (3R,4S,5R,6S,10R,11R,12R)-11-(acetyloxy)-1-(benzyloxy)-14-[formyl(methyl)amino]-5-hydroxy-4,6,10,12-tetramethyl-9-oxotetradecan-3-yl propanoate × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；298 K；0.1 M BIS-TRIS pH 5.5, 25% Polyethylene glycol 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.90 Å
R-free 0.271
|
|
4K43
Crystal structure of actin in complex with synthetic AplC tail analogue GC04 [N-{(1E,4R,5R,7E,9S,10S,11S)-4,10-dimethoxy-11-[(2S,4S,5S)-2-(4-methoxyphenyl)-5-methyl-1,3-dioxan-4-yl]-5,9-dimethyl-6-oxododeca-1,7-dien-1-yl}-N-methylformamide]
Deposited 2013-04-11
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain A
3–377（375 aa）
|
Not recorded
|
CA CALCIUM ION × 1
ADP ADENOSINE-5'-DIPHOSPHATE × 1
1PO N-{(1E,4R,5R,7E,9S,10S,11S)-4,10-dimethoxy-11-[(2S,4S,5S)-2-(4-methoxyphenyl)-5-methyl-1,3-dioxan-4-yl]-5,9-dimethyl-6-oxododeca-1,7-dien-1-yl}-N-methylformamide × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；298 K；0.1 M BIS-TRIS pH 5.5, 25% Polyethylene glycol 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.90 Å
R-free 0.277
|
|
4K43
Crystal structure of actin in complex with synthetic AplC tail analogue GC04 [N-{(1E,4R,5R,7E,9S,10S,11S)-4,10-dimethoxy-11-[(2S,4S,5S)-2-(4-methoxyphenyl)-5-methyl-1,3-dioxan-4-yl]-5,9-dimethyl-6-oxododeca-1,7-dien-1-yl}-N-methylformamide]
Deposited 2013-04-11
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain B
3–377（375 aa）
|
Not recorded
|
CA CALCIUM ION × 1
ADP ADENOSINE-5'-DIPHOSPHATE × 1
1PO N-{(1E,4R,5R,7E,9S,10S,11S)-4,10-dimethoxy-11-[(2S,4S,5S)-2-(4-methoxyphenyl)-5-methyl-1,3-dioxan-4-yl]-5,9-dimethyl-6-oxododeca-1,7-dien-1-yl}-N-methylformamide × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；298 K；0.1 M BIS-TRIS pH 5.5, 25% Polyethylene glycol 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.90 Å
R-free 0.277
|
|
4PKG
Complex of ATP-actin With the N-terminal Actin-Binding Domain of Tropomodulin
Deposited 2014-05-14
|
Different construct
Different mutation/modification
Different oligomeric state
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Insufficient information
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain A
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
CA CALCIUM ION × 3
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；0.18 M sodium fluoride, 11% w/v PEG3350, 1% v/v PEG1000, 1% v/v PEG400
|
Resolution 1.80 Å
R-free 0.187
|
|
4PKH
Complex of ADP-actin With the N-terminal Actin-Binding Domain of Tropomodulin
Deposited 2014-05-14
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Insufficient information
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain A
1–377（377 aa）
|
Not recorded
|
ADP ADENOSINE-5'-DIPHOSPHATE × 1
CA CALCIUM ION × 3
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；0.18 M sodium fluoride, 11% w/v PEG3350, 1% v/v PEG1000, 1% v/v PEG400
|
Resolution 2.15 Å
R-free 0.326
|
|
4PKH
Complex of ADP-actin With the N-terminal Actin-Binding Domain of Tropomodulin
Deposited 2014-05-14
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Insufficient information
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain D
1–377（377 aa）
|
Not recorded
|
ADP ADENOSINE-5'-DIPHOSPHATE × 1
CA CALCIUM ION × 3
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；0.18 M sodium fluoride, 11% w/v PEG3350, 1% v/v PEG1000, 1% v/v PEG400
|
Resolution 2.15 Å
R-free 0.326
|
|
4PKH
Complex of ADP-actin With the N-terminal Actin-Binding Domain of Tropomodulin
Deposited 2014-05-14
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 3
Insufficient information
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain F
1–377（377 aa）
|
Not recorded
|
ADP ADENOSINE-5'-DIPHOSPHATE × 1
CA CALCIUM ION × 3
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；0.18 M sodium fluoride, 11% w/v PEG3350, 1% v/v PEG1000, 1% v/v PEG400
|
Resolution 2.15 Å
R-free 0.326
|
|
4PKH
Complex of ADP-actin With the N-terminal Actin-Binding Domain of Tropomodulin
Deposited 2014-05-14
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 4
Insufficient information
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain I
1–377（377 aa）
|
Not recorded
|
ADP ADENOSINE-5'-DIPHOSPHATE × 1
CA CALCIUM ION × 3
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；0.18 M sodium fluoride, 11% w/v PEG3350, 1% v/v PEG1000, 1% v/v PEG400
|
Resolution 2.15 Å
R-free 0.326
|
|
4PKI
Complex of ATP-actin With the C-terminal Actin-Binding Domain of Tropomodulin
Deposited 2014-05-14
|
Different construct
Different mutation/modification
Different oligomeric state
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Insufficient information
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain A
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
CA CALCIUM ION × 3
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；0.25 M sodium chloride, 12% w/v PEG3350
|
Resolution 2.30 Å
R-free 0.201
|
|
4PL8
Structure of rabbit skeletal muscle actin in complex with a hybrid peptide comprising thymosin beta4 and the lysine-rich region of Cordon-Bleu
Deposited 2014-05-16
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Insufficient information
Heteromer；Protein × 3
PDB declaration: trimeric
|
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 2
CA CALCIUM ION × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 3.5；288 K；0.1 M citric acid, 15% (w/v) PEG 3350
|
Resolution 2.00 Å
R-free 0.207
|
|
4V0U
The crystal structure of ternary PP1G-PPP1R15B and G-actin complex
Deposited 2014-09-18
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 10
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain M
3–377（375 aa）
Fragment：RESIDUES 3-377
|
Not recorded
|
LAB LATRUNCULIN B × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7；0.2M CACL2, 0.1 M HEPES, PH7.0, 20% PEG6000
|
Resolution 7.88 Å
R-free 0.400
|
|
4V0U
The crystal structure of ternary PP1G-PPP1R15B and G-actin complex
Deposited 2014-09-18
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 6
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain A
3–377（375 aa）
Fragment：RESIDUES 3-377
|
Not recorded
|
LAB LATRUNCULIN B × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7；0.2M CACL2, 0.1 M HEPES, PH7.0, 20% PEG6000
|
Resolution 7.88 Å
R-free 0.400
|
|
4V0U
The crystal structure of ternary PP1G-PPP1R15B and G-actin complex
Deposited 2014-09-18
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 7
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain B
3–377（375 aa）
Fragment：RESIDUES 3-377
|
Not recorded
|
LAB LATRUNCULIN B × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7；0.2M CACL2, 0.1 M HEPES, PH7.0, 20% PEG6000
|
Resolution 7.88 Å
R-free 0.400
|
|
4V0U
The crystal structure of ternary PP1G-PPP1R15B and G-actin complex
Deposited 2014-09-18
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 8
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain C
3–377（375 aa）
Fragment：RESIDUES 3-377
|
Not recorded
|
LAB LATRUNCULIN B × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7；0.2M CACL2, 0.1 M HEPES, PH7.0, 20% PEG6000
|
Resolution 7.88 Å
R-free 0.400
|
|
4V0U
The crystal structure of ternary PP1G-PPP1R15B and G-actin complex
Deposited 2014-09-18
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 9
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain L
3–377（375 aa）
Fragment：RESIDUES 3-377
|
Not recorded
|
LAB LATRUNCULIN B × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7；0.2M CACL2, 0.1 M HEPES, PH7.0, 20% PEG6000
|
Resolution 7.88 Å
R-free 0.400
|
|
4WYB
Structure of the Bud6 flank domain in complex with actin
Deposited 2014-11-17
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain A
1–377（377 aa）
|
Not recorded
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
CA CALCIUM ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；3.5M sodium formate, 0.1M CaCl2, and 5 mM TCEP
|
Resolution 3.49 Å
R-free 0.258
|
|
4WYB
Structure of the Bud6 flank domain in complex with actin
Deposited 2014-11-17
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 10
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain S
1–377（377 aa）
|
Not recorded
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
CA CALCIUM ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；3.5M sodium formate, 0.1M CaCl2, and 5 mM TCEP
|
Resolution 3.49 Å
R-free 0.258
|
|
4WYB
Structure of the Bud6 flank domain in complex with actin
Deposited 2014-11-17
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 11
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain U
1–377（377 aa）
|
Not recorded
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
CA CALCIUM ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；3.5M sodium formate, 0.1M CaCl2, and 5 mM TCEP
|
Resolution 3.49 Å
R-free 0.258
|
|
4WYB
Structure of the Bud6 flank domain in complex with actin
Deposited 2014-11-17
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 12
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain X
1–377（377 aa）
|
Not recorded
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
CA CALCIUM ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；3.5M sodium formate, 0.1M CaCl2, and 5 mM TCEP
|
Resolution 3.49 Å
R-free 0.258
|
|
4WYB
Structure of the Bud6 flank domain in complex with actin
Deposited 2014-11-17
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain C
1–377（377 aa）
|
Not recorded
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
CA CALCIUM ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；3.5M sodium formate, 0.1M CaCl2, and 5 mM TCEP
|
Resolution 3.49 Å
R-free 0.258
|
|
4WYB
Structure of the Bud6 flank domain in complex with actin
Deposited 2014-11-17
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 3
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain E
1–377（377 aa）
|
Not recorded
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
CA CALCIUM ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；3.5M sodium formate, 0.1M CaCl2, and 5 mM TCEP
|
Resolution 3.49 Å
R-free 0.258
|
|
4WYB
Structure of the Bud6 flank domain in complex with actin
Deposited 2014-11-17
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 4
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain G
1–377（377 aa）
|
Not recorded
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
CA CALCIUM ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；3.5M sodium formate, 0.1M CaCl2, and 5 mM TCEP
|
Resolution 3.49 Å
R-free 0.258
|
|
4WYB
Structure of the Bud6 flank domain in complex with actin
Deposited 2014-11-17
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 5
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain I
1–377（377 aa）
|
Not recorded
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
CA CALCIUM ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；3.5M sodium formate, 0.1M CaCl2, and 5 mM TCEP
|
Resolution 3.49 Å
R-free 0.258
|
|
4WYB
Structure of the Bud6 flank domain in complex with actin
Deposited 2014-11-17
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 6
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain K
1–377（377 aa）
|
Not recorded
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
CA CALCIUM ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；3.5M sodium formate, 0.1M CaCl2, and 5 mM TCEP
|
Resolution 3.49 Å
R-free 0.258
|
|
4WYB
Structure of the Bud6 flank domain in complex with actin
Deposited 2014-11-17
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 7
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain M
1–377（377 aa）
|
Not recorded
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
CA CALCIUM ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；3.5M sodium formate, 0.1M CaCl2, and 5 mM TCEP
|
Resolution 3.49 Å
R-free 0.258
|
|
4WYB
Structure of the Bud6 flank domain in complex with actin
Deposited 2014-11-17
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 8
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain O
1–377（377 aa）
|
Not recorded
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
CA CALCIUM ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；3.5M sodium formate, 0.1M CaCl2, and 5 mM TCEP
|
Resolution 3.49 Å
R-free 0.258
|
|
4WYB
Structure of the Bud6 flank domain in complex with actin
Deposited 2014-11-17
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 9
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain Q
1–377（377 aa）
|
Not recorded
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
CA CALCIUM ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；3.5M sodium formate, 0.1M CaCl2, and 5 mM TCEP
|
Resolution 3.49 Å
R-free 0.258
|
|
4Z94
Actin Complex With a Chimera of Tropomodulin-1 and Leiomodin-1 Actin-Binding Site 2
Deposited 2015-04-09
|
Different construct
Different mutation/modification
Different oligomeric state
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Insufficient information
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain A
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
CA CALCIUM ION × 3
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.8；291.5 K；20% PEG3350, 200 mM lithium sulfate, 100 mM Tris, pH 8.8, 15% glycerol
|
Resolution 2.40 Å
R-free 0.243
|
|
5H53
The structure of rabbit skeletal muscle actomyosin rigor complex at 5.2 angstrom.
Deposited 2016-11-04
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 5
PDB declaration: pentameric
|
Chain D
3–377（375 aa）
Fragment：UNP residues 3-377
Chain E
3–377（375 aa）
Fragment：UNP residues 3-377
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 2
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.20 Å
|
|
5JLF
Structure of the F-actin-tropomyosin complex (Reprocessed)
Deposited 2016-04-27
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 7
PDB declaration: heptameric
|
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
MG MAGNESIUM ION × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；5 mM Tris-HCl pH 7.5, 1 mM DTT, 100 mM KCl, and 2 mM MgCl2
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was applied to a glow-discharged holey carbon grid, incubated for 10 s and manually blotted for 3 s from the backside with filter paper.
|
Resolution 3.60 Å
|
|
5KG8
Rigor myosin X co-complexed with an actin filament
Deposited 2016-06-12
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 4
PDB declaration: tetrameric
|
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 9.10 Å
|
|
5MVA
Structure of the thin filament at high calcium concentration
Deposited 2017-01-16
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 23
PDB declaration: 23-meric
|
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
Chain F
3–377（375 aa）
Chain G
3–377（375 aa）
Chain H
3–377（375 aa）
Chain I
3–377（375 aa）
Chain J
3–377（375 aa）
Chain K
3–377（375 aa）
Chain L
3–377（375 aa）
Chain M
3–377（375 aa）
Chain N
3–377（375 aa）
Chain O
3–377（375 aa）
Chain P
3–377（375 aa）
Chain Q
3–377（375 aa）
Chain R
3–377（375 aa）
Chain S
3–377（375 aa）
Chain T
3–377（375 aa）
Chain U
3–377（375 aa）
Chain V
3–377（375 aa）
Chain W
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 23
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
|
Resolution 27.70 Å
|
|
5MVY
Thin Filament at low calcium concentration
Deposited 2017-01-17
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 23
PDB declaration: 23-meric
|
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
Chain F
3–377（375 aa）
Chain G
3–377（375 aa）
Chain H
3–377（375 aa）
Chain I
3–377（375 aa）
Chain J
3–377（375 aa）
Chain K
3–377（375 aa）
Chain L
3–377（375 aa）
Chain M
3–377（375 aa）
Chain N
3–377（375 aa）
Chain O
3–377（375 aa）
Chain P
3–377（375 aa）
Chain Q
3–377（375 aa）
Chain R
3–377（375 aa）
Chain S
3–377（375 aa）
Chain T
3–377（375 aa）
Chain U
3–377（375 aa）
Chain V
3–377（375 aa）
Chain W
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 23
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
|
Resolution 28.40 Å
|
|
5ONV
Cryo-EM structure of F-actin in complex with ADP
Deposited 2017-08-04
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 5
PDB declaration: pentameric
|
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
MG MAGNESIUM ION × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；5 mM HEPES pH 7.5, 0.1 M KCl, 2 mM MgCl2, 2 mM, 2 mM NaN3, 1 mM TCEP and 0.2 mM ADP.
cryo-EM vitrification conditions
Cryogen ETHANE；Manual backside blotting using Whatman filter paper No.5.
|
Resolution 4.10 Å
|
|
5OOC
Cryo-EM structure of jasplakinolide-stabilized F-actin in complex with ADP
Deposited 2017-08-07
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 5
PDB declaration: pentameric
|
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
9ZK (4~{R},7~{R},10~{S},13~{S},15~{E},19~{S})-10-(4-azanylbutyl)-4-(4-hydroxyphenyl)-7-(1~{H}-indol-3-ylmethyl)-8,13,15,19-tetramethyl-1-oxa-5,8,11-triazacyclononadec-15-ene-2,6,9,12-tetrone × 5
MG MAGNESIUM ION × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；5 mM Tris pH 7.5, 2 mM NaN3, 1 mM DTT, 100 mM KCl and 2 mM MgCl2, 0.12 %(v/v) DMSO
cryo-EM vitrification conditions
Cryogen ETHANE；Manual backside blotting using Whatman filter paper No.5.
|
Resolution 3.60 Å
|
|
5OOD
Cryo-EM structure of jasplakinolide-stabilized F-actin in complex with ADP-Pi
Deposited 2017-08-07
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 5
PDB declaration: pentameric
|
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
PO4 PHOSPHATE ION × 5
9ZK (4~{R},7~{R},10~{S},13~{S},15~{E},19~{S})-10-(4-azanylbutyl)-4-(4-hydroxyphenyl)-7-(1~{H}-indol-3-ylmethyl)-8,13,15,19-tetramethyl-1-oxa-5,8,11-triazacyclononadec-15-ene-2,6,9,12-tetrone × 5
MG MAGNESIUM ION × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；5 mM Tris pH 7.5, 2 mM NaN3, 1 mM DTT, 100 mM KCl and 2 mM MgCl2, 9.0 %(v/v) DMSO
cryo-EM vitrification conditions
Cryogen ETHANE；Manual backside blotting using Whatman filter paper No.5.
|
Resolution 3.70 Å
|
|
5OOE
Cryo-EM structure of F-actin in complex with AppNHp (AMPPNP)
Deposited 2017-08-07
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 5
PDB declaration: pentameric
|
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 5
MG MAGNESIUM ION × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；5 mM HEPES pH 7.5, 0.1 M KCl, 2 mM MgCl2, 2 mM, 2 mM NaN3, 0.5 mM TCEP and 0.4 mM AppNHp.
cryo-EM vitrification conditions
Cryogen ETHANE；Manual backside blotting using Whatman filter paper No.5.
|
Resolution 3.60 Å
|
|
5OOF
Cryo-EM structure of F-actin in complex with ADP-BeFx
Deposited 2017-08-07
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 5
PDB declaration: pentameric
|
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
MG MAGNESIUM ION × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；5 mM HEPES pH 7.5, 0.1 M KCl, 2 mM MgCl2, 2 mM, 2 mM NaN3, 1 mM TCEP, 0.2 mM ADP, 0.2 mM BeF2 and 5 mM NaF.
cryo-EM vitrification conditions
Cryogen ETHANE；Manual backside blotting using Whatman filter paper No.5.
|
Resolution 3.40 Å
|
|
5UBO
Mical-oxidized Actin complex with Gelsolin Segment 1
Deposited 2016-12-21
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain A
1–377（377 aa）
Fragment：unp residues 1-377
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
CA CALCIUM ION × 3
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.6；298 K；PEG 6000, NaCl, imidazole, ATP, calcium
|
Resolution 2.39 Å
R-free 0.191
|
|
5YEE
Crystal structure of LokiProfilin1/Rabbit Actin Complex
Deposited 2017-09-16
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain B
1–377（377 aa）
|
Not recorded
|
LAB LATRUNCULIN B × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；100 mM Hepes, pH 7.0, 20 % w/v Polyethylene glycol 6,000, 200 mM NaCl, 10 mM ATP disodium salt
|
Resolution 1.81 Å
R-free 0.249
|
|
5YPU
Crystal structure of an actin monomer in complex with the nucleator Cordon-Bleu MET72NLE WH2-motif peptide
Deposited 2017-11-03
|
Different construct
Different oligomeric state
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain A
7–374（368 aa）
|
Not recorded
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
CA CALCIUM ION × 3
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.9；298.15 K；20mM MES pH 4.9, 0.2mM CaCl2,2H2O, 20%(w/v) PEG 3,350
|
Resolution 2.00 Å
R-free 0.240
|
|
5YPU
Crystal structure of an actin monomer in complex with the nucleator Cordon-Bleu MET72NLE WH2-motif peptide
Deposited 2017-11-03
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain C
7–374（368 aa）
|
Not recorded
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
CA CALCIUM ION × 4
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.9；298.15 K；20mM MES pH 4.9, 0.2mM CaCl2,2H2O, 20%(w/v) PEG 3,350
|
Resolution 2.00 Å
R-free 0.240
|
|
5ZZA
OdinProfilin/Rabbit Actin Complex
Deposited 2018-05-31
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain A
5–377（373 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
LAB LATRUNCULIN B × 2
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
CA CALCIUM ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5；291 K；100 mM Citrate, 20% PEG6000
|
Resolution 1.53 Å
R-free 0.179
|
|
5ZZB
LokiProfilin2/Rabbit Actin Complex
Deposited 2018-05-31
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain B
7–377（371 aa）
|
Not recorded
|
LAB LATRUNCULIN B × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
CA CALCIUM ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；100 mM PCTP
25% PEG1500
|
Resolution 2.30 Å
R-free 0.274
|
|
5ZZB
LokiProfilin2/Rabbit Actin Complex
Deposited 2018-05-31
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain D
7–377（371 aa）
|
Not recorded
|
LAB LATRUNCULIN B × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
CA CALCIUM ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；100 mM PCTP
25% PEG1500
|
Resolution 2.30 Å
R-free 0.274
|
|
6AV9
CryoEM structure of Mical Oxidized Actin (Class 1)
Deposited 2017-09-01
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 3
PDB declaration: trimeric
|
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 3
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blot Force 1, Blot time 4s
|
Resolution 3.90 Å
|
|
6AVB
CryoEM structure of Mical Oxidized Actin (Class 1)
Deposited 2017-09-01
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 3
PDB declaration: trimeric
|
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 3
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blot Force 1, Blot time 4s
|
Resolution 3.90 Å
|
|
6BIH
The Structure of the Actin-Smooth Muscle Myosin Motor Domain Complex in the Rigor State
Deposited 2017-11-02
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 14
PDB declaration: tetradecameric
|
Chain C
1–377（377 aa）
|
Not recorded
|
ADP ADENOSINE-5'-DIPHOSPHATE × 7
MG MAGNESIUM ION × 7
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；actin buffer: 10 mM imidazole, 10 mM KCl, 1.0 mM MgCl2, 1.0 mM EGTA, 0.5 mM DTT, pH 7.4, myosin buffer: 10 mM imidazole, 10 mM KCl, 1.0 mM MgCl2, 1.0 mM EGTA, 0.5 mM DTT, pH 7.0
cryo-EM vitrification conditions
Cryogen ETHANE；Some specimens were frozen manually using a homemade plunger.
|
Resolution 6.00 Å
|
|
6BIH
The Structure of the Actin-Smooth Muscle Myosin Motor Domain Complex in the Rigor State
Deposited 2017-11-02
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain C
1–377（377 aa）
|
Not recorded
|
ADP ADENOSINE-5'-DIPHOSPHATE × 1
MG MAGNESIUM ION × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；actin buffer: 10 mM imidazole, 10 mM KCl, 1.0 mM MgCl2, 1.0 mM EGTA, 0.5 mM DTT, pH 7.4, myosin buffer: 10 mM imidazole, 10 mM KCl, 1.0 mM MgCl2, 1.0 mM EGTA, 0.5 mM DTT, pH 7.0
cryo-EM vitrification conditions
Cryogen ETHANE；Some specimens were frozen manually using a homemade plunger.
|
Resolution 6.00 Å
|
|
6BNO
Structure of bare actin filament
Deposited 2017-11-17
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 8
PDB declaration: octameric
|
Chain A
1–373（373 aa）
Chain B
1–373（373 aa）
Chain C
1–373（373 aa）
Chain D
1–373（373 aa）
Chain E
1–373（373 aa）
Chain F
1–373（373 aa）
Chain G
1–373（373 aa）
Chain H
1–373（373 aa）
|
Not recorded
|
MG MAGNESIUM ION × 8
ADP ADENOSINE-5'-DIPHOSPHATE × 8
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；Buffer was filtered through 0.44 um filter and degassed.
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was applied to a glow-discharged holey carbon grid, incubated for 60 seconds and blotted for 3 seconds from the backside with filter paper.
|
Resolution 5.50 Å
|
|
6BNP
CryoEM structure of MyosinVI-actin complex in the rigor (nucleotide-free) state
Deposited 2017-11-17
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 14
PDB declaration: tetradecameric
|
Chain A
1–373（373 aa）
Chain B
1–373（373 aa）
Chain C
1–373（373 aa）
Chain D
1–373（373 aa）
Chain E
1–373（373 aa）
Chain F
1–373（373 aa）
Chain G
1–373（373 aa）
Chain H
1–373（373 aa）
|
Not recorded
|
MG MAGNESIUM ION × 8
ADP ADENOSINE-5'-DIPHOSPHATE × 8
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；Buffer was filtered through 0.44 um filter and degassed.
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was applied to a glow-discharged holey carbon grid. 3 uL actin was incubated for 60 seconds. 3 uL of myosin VI was added and incubated for 60 seconds. 3 uL solution was removed. An additional 3 uL of myosin VI was applied. After 60 seconds, 3 uL solution was removed, and the grid was blotted for 3 seconds from the backside with filter paper.
|
Resolution 4.60 Å
|
|
6BNQ
CryoEM structure of Myosin VI-Actin complex in the ADP state
Deposited 2017-11-17
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 14
PDB declaration: tetradecameric
|
Chain A
1–373（373 aa）
Chain B
1–373（373 aa）
Chain C
1–373（373 aa）
Chain D
1–373（373 aa）
Chain E
1–373（373 aa）
Chain F
1–373（373 aa）
Chain G
1–373（373 aa）
Chain H
1–373（373 aa）
|
Not recorded
|
MG MAGNESIUM ION × 8
ADP ADENOSINE-5'-DIPHOSPHATE × 8
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；Buffer was filtered through 0.44 um filter and degassed.
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was applied to a glow-discharged holey carbon grid. 3 uL actin was incubated for 60 seconds. 3 uL of myosin VI was added and incubated for 60 seconds. 3 uL solution was removed. An additional 3 uL of myosin VI was applied. After 60 seconds, 3 uL solution was removed, and the grid was blotted for 3 seconds from the backside with filter paper.
|
Resolution 5.50 Å
|
|
6BNU
Structure of bare actin filament, backbone-averaged with sidechains truncated to alanine
Deposited 2017-11-17
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 8
PDB declaration: octameric
|
Chain A
1–373（373 aa）
Chain B
1–373（373 aa）
Chain C
1–373（373 aa）
Chain D
1–373（373 aa）
Chain E
1–373（373 aa）
Chain F
1–373（373 aa）
Chain G
1–373（373 aa）
Chain H
1–373（373 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；Buffer was filtered through 0.44 um filter and degassed.
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was applied to a glow-discharged holey carbon grid, incubated for 60 seconds, and blotted for 3 seconds from the backside with filter paper.
|
Resolution 7.50 Å
|
|
6BNV
CryoEM structure of MyosinVI-actin complex in the rigor (nucleotide-free) state, backbone-averaged with side chains truncated to alanine
Deposited 2017-11-17
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 20
PDB declaration: eicosameric
|
Chain A
1–373（373 aa）
Chain B
1–373（373 aa）
Chain C
1–373（373 aa）
Chain D
1–373（373 aa）
Chain E
1–373（373 aa）
Chain F
1–373（373 aa）
Chain G
1–373（373 aa）
Chain H
1–373（373 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；Buffer was filtered through 0.44 um filter and degassed.
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was applied to a glow-discharged holey carbon grid. 3 uL actin was incubated for 60 seconds. 3 uL of myosin VI was added and incubated for 60 seconds. 3 uL solution was removed. An additional 3 uL of myosin VI was applied. After 60 seconds, 3 uL solution was removed, and the grid was blotted for 3 seconds from the backside with filter paper.
|
Resolution 4.60 Å
|
|
6BNW
CryoEM structure of Myosin VI-Actin complex in the ADP state, backbone-averaged with side chains truncated to alanine
Deposited 2017-11-17
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 14
PDB declaration: tetradecameric
|
Chain A
1–373（373 aa）
Chain B
1–373（373 aa）
Chain C
1–373（373 aa）
Chain D
1–373（373 aa）
Chain E
1–373（373 aa）
Chain F
1–373（373 aa）
Chain G
1–373（373 aa）
Chain H
1–373（373 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；Buffer was filtered through 0.44 um filter and degassed
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was applied to a glow-discharged holey carbon grid. 3 uL actin was incubated for 60 seconds. 3 uL of myosin VI was added and incubated for 60 seconds. 3 uL solution was removed. An additional 3 uL of myosin VI was applied. After 60 seconds, 3 uL solution was removed, and the grid was blotted for 3 seconds from the backside with filter paper.
|
Resolution 5.50 Å
|
|
6C1D
High-Resolution Cryo-EM Structures of Actin-bound Myosin States Reveal the Mechanism of Myosin Force Sensing
Deposited 2018-01-04
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 8
PDB declaration: heptameric
|
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Not recorded
|
ADP ADENOSINE-5'-DIPHOSPHATE × 6
MG MAGNESIUM ION × 6
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å
|
|
6C1G
High-Resolution Cryo-EM Structures of Actin-bound Myosin States Reveal the Mechanism of Myosin Force Sensing
Deposited 2018-01-04
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 7
PDB declaration: heptameric
|
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Not recorded
|
MG MAGNESIUM ION × 6
ADP ADENOSINE-5'-DIPHOSPHATE × 6
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å
|
|
6C1H
High-Resolution Cryo-EM Structures of Actin-bound Myosin States Reveal the Mechanism of Myosin Force Sensing
Deposited 2018-01-04
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 7
PDB declaration: heptameric
|
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Not recorded
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
MG MAGNESIUM ION × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å
|
|
6FHL
Cryo-EM structure of F-actin in complex with ADP-Pi
Deposited 2018-01-15
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 5
PDB declaration: pentameric
|
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
MG MAGNESIUM ION × 5
PO4 PHOSPHATE ION × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；5 mM HEPES pH 7.5, 0.05 M KCl, 2 mM MgCl2, 2 mM NaN3, 0.5 mM TCEP, 0.2 mM ADP, 50 mM potassium phosphate.
cryo-EM vitrification conditions
Cryogen ETHANE；8s blotting, 1s drain time, -25 force
|
Resolution 3.30 Å
|
|
6FM2
CARP domain of mouse cyclase-associated protein 1 (CAP1) bound to ADP-actin
Deposited 2018-01-30
|
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 4
PDB declaration: tetrameric
|
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
MG MAGNESIUM ION × 2
ADP ADENOSINE-5'-DIPHOSPHATE × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；0.1 M Tris-HCl, 0.2 M LiCl, 20% (w/v) PEG8000
|
Resolution 2.80 Å
R-free 0.234
|
|
6GVC
Structure of ArhGAP12 bound to G-Actin
Deposited 2018-06-20
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain B
1–377（377 aa）
|
Not recorded
|
EDO 1,2-ETHANEDIOL × 2
LAB LATRUNCULIN B × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；20% PEG 3350,
0.2M Sodium Thiocyanate
0.1 M Bis Tris Propane pH6.5
|
Resolution 2.60 Å
R-free 0.251
|
|
6GVC
Structure of ArhGAP12 bound to G-Actin
Deposited 2018-06-20
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain A
1–377（377 aa）
|
Not recorded
|
EDO 1,2-ETHANEDIOL × 3
LAB LATRUNCULIN B × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；20% PEG 3350,
0.2M Sodium Thiocyanate
0.1 M Bis Tris Propane pH6.5
|
Resolution 2.60 Å
R-free 0.251
|
|
6GVC
Structure of ArhGAP12 bound to G-Actin
Deposited 2018-06-20
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 3
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain C
1–377（377 aa）
|
Not recorded
|
EDO 1,2-ETHANEDIOL × 1
LAB LATRUNCULIN B × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；20% PEG 3350,
0.2M Sodium Thiocyanate
0.1 M Bis Tris Propane pH6.5
|
Resolution 2.60 Å
R-free 0.251
|
|
6GVC
Structure of ArhGAP12 bound to G-Actin
Deposited 2018-06-20
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 4
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain D
1–377（377 aa）
|
Not recorded
|
EDO 1,2-ETHANEDIOL × 2
LAB LATRUNCULIN B × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；20% PEG 3350,
0.2M Sodium Thiocyanate
0.1 M Bis Tris Propane pH6.5
|
Resolution 2.60 Å
R-free 0.251
|
|
6JBK
Crystal structure of an actin monomer in complex with the nucleator Cordon-Bleu WH2-motif peptide mutant. T22V
Deposited 2019-01-25
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain A
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
CA CALCIUM ION × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；298.15 K；20mM MES, pH 4.5, 0.2mM CaCl2.2H2O, 11% PEG 3350
|
Resolution 2.45 Å
R-free 0.239
|
|
6JBK
Crystal structure of an actin monomer in complex with the nucleator Cordon-Bleu WH2-motif peptide mutant. T22V
Deposited 2019-01-25
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
CA CALCIUM ION × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；298.15 K；20mM MES, pH 4.5, 0.2mM CaCl2.2H2O, 11% PEG 3350
|
Resolution 2.45 Å
R-free 0.239
|
|
6JBK
Crystal structure of an actin monomer in complex with the nucleator Cordon-Bleu WH2-motif peptide mutant. T22V
Deposited 2019-01-25
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 3
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain E
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
CA CALCIUM ION × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；298.15 K；20mM MES, pH 4.5, 0.2mM CaCl2.2H2O, 11% PEG 3350
|
Resolution 2.45 Å
R-free 0.239
|
|
6JBK
Crystal structure of an actin monomer in complex with the nucleator Cordon-Bleu WH2-motif peptide mutant. T22V
Deposited 2019-01-25
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 4
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain G
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
CA CALCIUM ION × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；298.15 K；20mM MES, pH 4.5, 0.2mM CaCl2.2H2O, 11% PEG 3350
|
Resolution 2.45 Å
R-free 0.239
|
|
6JCU
Crystal structure of an actin monomer in complex with a nucleator Cordon-Bleu WH2-motif peptide mutant. T22V, H11R
Deposited 2019-01-30
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain A
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
CA CALCIUM ION × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298.15 K；0.2M Ammonium nitrate, 20% PEG 3350
|
Resolution 2.30 Å
R-free 0.230
|
|
6JCU
Crystal structure of an actin monomer in complex with a nucleator Cordon-Bleu WH2-motif peptide mutant. T22V, H11R
Deposited 2019-01-30
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
CA CALCIUM ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298.15 K；0.2M Ammonium nitrate, 20% PEG 3350
|
Resolution 2.30 Å
R-free 0.230
|
|
6JH8
Crystal structure of an actin monomer in complex with a chimeric peptide of Cordon-Bleu WH2 mutant and MIM.
Deposited 2019-02-17
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain A
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
CA CALCIUM ION × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；298.15 K；0.1M sodium acetate trihydrate, pH 4.6, 10% PEG 4000
|
Resolution 2.15 Å
R-free 0.229
|
|
6JH9
Crystal structure of an actin monomer in complex with a chimeric peptide of Cordon-Bleu WH2 mutant and MIM. Lys18Arg
Deposited 2019-02-17
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain A
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
CA CALCIUM ION × 2
GOL GLYCEROL × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.8；298.15 K；0.1M sodium acetate trihydrate, pH 4.8, 8% PEG 4000
|
Resolution 1.74 Å
R-free 0.206
|
|
6KN7
Structure of human cardiac thin filament in the calcium free state
Deposited 2019-08-03
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 29
PDB declaration: 29-meric
|
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
Chain F
3–377（375 aa）
Chain G
3–377（375 aa）
Chain H
3–377（375 aa）
Chain I
3–377（375 aa）
Chain J
3–377（375 aa）
Chain K
3–377（375 aa）
Chain L
3–377（375 aa）
Chain M
3–377（375 aa）
Chain N
3–377（375 aa）
Chain O
3–377（375 aa）
|
Not recorded
|
ADP ADENOSINE-5'-DIPHOSPHATE × 15
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.60 Å
|
|
6KN8
Structure of human cardiac thin filament in the calcium bound state
Deposited 2019-08-03
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 29
PDB declaration: 29-meric
|
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
Chain F
3–377（375 aa）
Chain G
3–377（375 aa）
Chain H
3–377（375 aa）
Chain I
3–377（375 aa）
Chain J
3–377（375 aa）
Chain K
3–377（375 aa）
Chain L
3–377（375 aa）
Chain M
3–377（375 aa）
Chain N
3–377（375 aa）
Chain O
3–377（375 aa）
|
Not recorded
|
ADP ADENOSINE-5'-DIPHOSPHATE × 15
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.80 Å
|
|
6MGO
Structure of rabbit actin in complex with Mycalolide B
Deposited 2018-09-14
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain A
1–377（377 aa）
|
Not recorded
|
JQV Mycalolide B × 1
CA CALCIUM ION × 1
ADP ADENOSINE-5'-DIPHOSPHATE × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；PEG 8000, magnesium acetate, cacodylate
|
Resolution 2.20 Å
R-free 0.195
|
|
6NAS
Ternary Complex of Ac-Alpha-Actin with Profilin and AcCoA-NAA80
Deposited 2018-12-06
|
Different mutation/modification
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 3
PDB declaration: trimeric
|
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
CA CALCIUM ION × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
LAB LATRUNCULIN B × 1
GOL GLYCEROL × 3
ACO ACETYL COENZYME *A × 1
MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；290 K；16% Peg3350, 0.1 MES pH 6.5, 0.2M NH4NO3
|
Resolution 2.90 Å
R-free 0.239
|
|
6NBE
Ternary Complex of Ac-Alpha-Actin with Profilin and CoA-NAA80
Deposited 2018-12-07
|
Different mutation/modification
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 3
PDB declaration: trimeric
|
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
CA CALCIUM ION × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
LAB LATRUNCULIN B × 1
GOL GLYCEROL × 3
COA COENZYME A × 1
MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；290 K；16% Peg3350, 0.1 MES pH 6.5, 0.2M NH4NO3
|
Resolution 2.00 Å
R-free 0.184
|
|
6QRI
Structure of rabbit G-actin in complex with chivosazole A
Deposited 2019-02-19
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain B
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 1
CV9 (2~{R},3~{R},5~{S},6~{E},8~{E},10~{Z},12~{S},13~{R},16~{Z},18~{E},20~{Z},22~{E},24~{R},25~{S},26~{E},28~{Z})-13-[(2~{S},3~{S},5~{S})-3,5-bis(oxidanyl)hexan-2-yl]-25-[(2~{R},3~{R},4~{S},5~{R},6~{R})-3,4-dimethoxy-6-methyl-5-oxidanyl-oxan-2-yl]oxy-3-methoxy-2,12,22,24-tetramethyl-5-oxidanyl-14,32-dioxa-33-azabicyclo[28.2.1]tritriaconta-1(33),6,8,10,16,18,20,22,26,28,30-undecaen-15-one × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；0.5 M lithium chloride, 100 mM Tris(hydroxymethyl)aminomethane hydrochloride (Tris-HCl), 28 % (w/v) polyethylenglycol 6000, pH 8.5
|
Resolution 2.40 Å
R-free 0.268
|
|
6QRI
Structure of rabbit G-actin in complex with chivosazole A
Deposited 2019-02-19
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain A
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 1
CV9 (2~{R},3~{R},5~{S},6~{E},8~{E},10~{Z},12~{S},13~{R},16~{Z},18~{E},20~{Z},22~{E},24~{R},25~{S},26~{E},28~{Z})-13-[(2~{S},3~{S},5~{S})-3,5-bis(oxidanyl)hexan-2-yl]-25-[(2~{R},3~{R},4~{S},5~{R},6~{R})-3,4-dimethoxy-6-methyl-5-oxidanyl-oxan-2-yl]oxy-3-methoxy-2,12,22,24-tetramethyl-5-oxidanyl-14,32-dioxa-33-azabicyclo[28.2.1]tritriaconta-1(33),6,8,10,16,18,20,22,26,28,30-undecaen-15-one × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；0.5 M lithium chloride, 100 mM Tris(hydroxymethyl)aminomethane hydrochloride (Tris-HCl), 28 % (w/v) polyethylenglycol 6000, pH 8.5
|
Resolution 2.40 Å
R-free 0.268
|
|
6RSW
HFD domain of mouse CAP1 bound to the pointed end of G-actin
Deposited 2019-05-22
|
Different mutation/modification
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 3
PDB declaration: trimeric
|
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
MG MAGNESIUM ION × 2
ADP ADENOSINE-5'-DIPHOSPHATE × 1
EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；0.1M HEPES, 0.1 mM KCl, 10% PEG4000 (w/v)
|
Resolution 1.95 Å
R-free 0.194
|
|
6T1Y
Cryo-EM structure of phalloidin-stabilized F-actin (copolymerized)
Deposited 2019-10-07
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 10
PDB declaration: decameric
|
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
MG MAGNESIUM ION × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；5 mM Tris pH 7.5, 2 mM NaN3, 1 mM DTT, 100 mM KCl and 2 mM MgCl2, 0.01 %(v/v) MeOH, 0.02 %(v/w) Tween 20
cryo-EM vitrification conditions
Cryogen ETHANE；Manual backside blotting using Whatman filter paper No.5.
|
Resolution 3.30 Å
|
|
6T20
Cryo-EM structure of phalloidin-stabilized F-actin (aged)
Deposited 2019-10-07
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 10
PDB declaration: decameric
|
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
MG MAGNESIUM ION × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；5 mM Tris pH 7.5, 2 mM NaN3, 1 mM DTT, 100 mM KCl and 2 mM MgCl2, 2.0 %(v/v) MeOH, 0.03 %(v/w) Tween 20
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å
|
|
6T23
Cryo-EM structure of jasplakinolide-stabilized F-actin (aged)
Deposited 2019-10-07
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 5
PDB declaration: pentameric
|
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
PO4 PHOSPHATE ION × 5
MG MAGNESIUM ION × 5
9ZK (4~{R},7~{R},10~{S},13~{S},15~{E},19~{S})-10-(4-azanylbutyl)-4-(4-hydroxyphenyl)-7-(1~{H}-indol-3-ylmethyl)-8,13,15,19-tetramethyl-1-oxa-5,8,11-triazacyclononadec-15-ene-2,6,9,12-tetrone × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；5 mM Tris pH 7.5, 2 mM NaN3, 1 mM DTT, 100 mM KCl and 2 mM MgCl2, 0.2 %(v/v) DMSO, 0.02 %(v/w) Tween 20
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å
|
|
6T24
Cryo-EM structure of jasplakinolide-stabilized F-actin (aged)
Deposited 2019-10-07
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 5
PDB declaration: pentameric
|
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
PO4 PHOSPHATE ION × 5
MG MAGNESIUM ION × 5
9ZK (4~{R},7~{R},10~{S},13~{S},15~{E},19~{S})-10-(4-azanylbutyl)-4-(4-hydroxyphenyl)-7-(1~{H}-indol-3-ylmethyl)-8,13,15,19-tetramethyl-1-oxa-5,8,11-triazacyclononadec-15-ene-2,6,9,12-tetrone × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；5 mM Tris pH 7.5, 2 mM NaN3, 1 mM DTT, 100 mM KCl and 2 mM MgCl2, 0.5 %(v/v) DMSO, 0.02 %(v/w) Tween 20
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å
|
|
6T25
Cryo-EM structure of phalloidin-Alexa Flour-546-stabilized F-actin (copolymerized)
Deposited 2019-10-07
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 10
PDB declaration: decameric
|
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
MG MAGNESIUM ION × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；5 mM Tris pH 7.5, 2 mM NaN3, 1 mM DTT, 100 mM KCl and 2 mM MgCl2, 1.5 %(v/v) MeOH, 0.02 %(v/w) Tween 20
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å
|
|
6U96
Actin phalloidin at BeFx state
Deposited 2019-09-06
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 10
PDB declaration: decameric
|
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；pH7.4, 10mM Tris, 50mM KCl, 1 mM MgC2, 0.2mM CaCl2, 1mM ATP, 1mM DTT, 0.2mM EGTA, 0.2mM BeCl2, 5mM NaF
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å
|
|
6UBY
Isolated cofilin bound to an actin filament
Deposited 2019-09-13
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 9
PDB declaration: nonameric
|
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
Chain H
1–377（377 aa）
|
Not recorded
|
MG MAGNESIUM ION × 7
ADP ADENOSINE-5'-DIPHOSPHATE × 7
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.50 Å
|
|
6UC0
Isolated S3D-cofilin bound to an actin filament
Deposited 2019-09-13
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 8
PDB declaration: octameric
|
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
|
Not recorded
|
MG MAGNESIUM ION × 7
ADP ADENOSINE-5'-DIPHOSPHATE × 7
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.50 Å
|
|
6UC4
Barbed end side of a cofilactin cluster
Deposited 2019-09-13
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 16
PDB declaration: hexadecameric
|
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
Chain H
1–377（377 aa）
Chain J
1–377（377 aa）
Chain K
1–377（377 aa）
Chain L
1–377（377 aa）
|
Not recorded
|
MG MAGNESIUM ION × 9
ADP ADENOSINE-5'-DIPHOSPHATE × 9
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 9.20 Å
|
|
6VAO
Human cofilin-1 decorated actin filament
Deposited 2019-12-17
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 10
PDB declaration: decameric
|
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
|
Not recorded
|
MG MAGNESIUM ION × 5
ADP ADENOSINE-5'-DIPHOSPHATE × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å
|
|
6VAU
Bare actin filament from a partially cofilin-decorated sample
Deposited 2019-12-17
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 5
PDB declaration: pentameric
|
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
|
Not recorded
|
MG MAGNESIUM ION × 5
ADP ADENOSINE-5'-DIPHOSPHATE × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å
|
|
6VEC
Cryo-EM structure of F-actin/Plastin2-ABD2 complex
Deposited 2019-12-31
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 22
PDB declaration: 22-meric
|
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
Chain H
1–377（377 aa）
Chain I
1–377（377 aa）
Chain J
1–377（377 aa）
Chain K
1–377（377 aa）
|
Not recorded
|
ADP ADENOSINE-5'-DIPHOSPHATE × 11
MG MAGNESIUM ION × 11
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å
|
|
6W17
Structure of Dip1-activated Arp2/3 complex with nucleated actin filament
Deposited 2020-03-03
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 17
PDB declaration: heptadecameric
|
Chain I
1–377（377 aa）
Chain J
1–377（377 aa）
Chain K
1–377（377 aa）
Chain L
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
MG MAGNESIUM ION × 6
ATP ADENOSINE-5'-TRIPHOSPHATE × 2
ADP ADENOSINE-5'-DIPHOSPHATE × 4
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å
|
|
6W7V
Structure of rabbit actin in complex with truncated analog of Mycalolide B
Deposited 2020-03-19
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain A
1–377（377 aa）
|
Not recorded
|
CA CALCIUM ION × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
LAB LATRUNCULIN B × 1
EDO 1,2-ETHANEDIOL × 3
TFJ (1E,3R,4R,5S,6R,9S,10S,12S)-12-[(4-aminobutanoyl)oxy]-1-[ethyl(formyl)amino]-4,10-dimethoxy-3,5,9,13-tetramethyltetradec-1-en-6-yl (2R)-oxolane-2-carboxylate × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；0.1M Tris-HCl, pH 8.5, 25% PEG3350
|
Resolution 1.70 Å
R-free 0.213
|
|
6WVT
Structural basis of alphaE-catenin - F-actin catch bond behavior
Deposited 2020-05-06
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 12
PDB declaration: dodecameric
|
Chain B
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
Chain H
1–377（377 aa）
Chain I
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
MG MAGNESIUM ION × 6
ADP ADENOSINE-5'-DIPHOSPHATE × 6
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.56 Å
|
|
6YP9
Rabbit muscle actin in complex with ADF-H and ATP-ATTO-488
Deposited 2020-04-15
|
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
CA CALCIUM ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；0.1 M sodium cacodylate (pH 6.0) and 15% (w/v) PEG 4000
|
Resolution 2.56 Å
R-free 0.230
|
|
7AD9
Structure of the Lifeact-F-actin complex
Deposited 2020-09-14
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 15
PDB declaration: pentadecameric
|
Chain B
1–377（377 aa）
Chain D
1–377（377 aa）
Chain F
1–377（377 aa）
Chain H
1–377（377 aa）
Chain I
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
MG MAGNESIUM ION × 5
PO4 PHOSPHATE ION × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8；120 mM KCl, 20 mM Tris pH 8, 2 mM MgCl2, 1 mM DTT, and 0.02% w/v Tween-20
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å
|
|
7AHN
Cryo-EM structure of F-actin stabilized by cis-optoJASP-8
Deposited 2020-09-24
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 5
PDB declaration: pentameric
|
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
MG MAGNESIUM ION × 5
PO4 PHOSPHATE ION × 5
RLZ ~{N}-[4-[(4~{R},7~{R},10~{S},13~{S},15~{E},19~{S})-4-(4-hydroxyphenyl)-7-(1~{H}-indol-3-ylmethyl)-8,13,15,19-tetramethyl-2,6,9,12-tetrakis(oxidanylidene)-1-oxa-5,8,11-triazacyclononadec-15-en-10-yl]butyl]-~{N}'-[5-methoxy-2-[(~{Z})-(3,4,5-trimethoxyphenyl)diazenyl]phenyl]butanediamide × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；5 mM Tris pH 7.5, 2 mM NaN3, 1 mM DTT, 100 mM KCl and 2 mM MgCl2, 0.4 %(v/v) DMSO, 0.02 %(v/w) Tween 20
cryo-EM vitrification conditions
Cryogen ETHANE；1.5 mul sample, automatic blotting for 7-7.5s, blot force -25, drain time 1s.
|
Resolution 2.90 Å
|
|
7AHQ
Cryo-EM structure of F-actin stabilized by trans-optoJASP-8
Deposited 2020-09-25
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 5
PDB declaration: pentameric
|
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
PO4 PHOSPHATE ION × 5
MG MAGNESIUM ION × 5
RLZ ~{N}-[4-[(4~{R},7~{R},10~{S},13~{S},15~{E},19~{S})-4-(4-hydroxyphenyl)-7-(1~{H}-indol-3-ylmethyl)-8,13,15,19-tetramethyl-2,6,9,12-tetrakis(oxidanylidene)-1-oxa-5,8,11-triazacyclononadec-15-en-10-yl]butyl]-~{N}'-[5-methoxy-2-[(~{Z})-(3,4,5-trimethoxyphenyl)diazenyl]phenyl]butanediamide × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；5 mM Tris pH 7.5, 2 mM NaN3, 1 mM DTT, 100 mM KCl and 2 mM MgCl2, 0.7 %(v/v) DMSO, 0.02 %(v/w) Tween 20
cryo-EM vitrification conditions
Cryogen ETHANE；1.5 mul sample, automatic blotting for 7-7.5s, blot force -25, drain time 1s.
|
Resolution 3.60 Å
|
|
7C2F
Crystal Structure of the Thorarchaeota ProGel/rabbit actin complex
Deposited 2020-05-07
|
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain A
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
LAB LATRUNCULIN B × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；291 K；0.1 M MES pH 6.0
0.2 M magnesium chloride hexahydrate
20% w/v polyethylene glycol 6000
|
Resolution 2.03 Å
R-free 0.231
|
|
7C2F
Crystal Structure of the Thorarchaeota ProGel/rabbit actin complex
Deposited 2020-05-07
|
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain C
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
LAB LATRUNCULIN B × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；291 K；0.1 M MES pH 6.0
0.2 M magnesium chloride hexahydrate
20% w/v polyethylene glycol 6000
|
Resolution 2.03 Å
R-free 0.231
|
|
7C2G
Crystal Structure of the Thorarchaeota 2DGel/rabbit actin complex
Deposited 2020-05-07
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain A
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
CA CALCIUM ION × 7
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；291 K；0.1 M Bis-Tris pH 5.5
0.2 M magnesium chloride hexahydrate
25% w/v polyethylene glycol 3350
|
Resolution 1.71 Å
R-free 0.203
|
|
7C2H
Crystal Structure of the Thorarchaeota 2DGel3/rabbit actin complex
Deposited 2020-05-07
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain A
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
CA CALCIUM ION × 6
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；291 K；0.1 M CHES pH 9.5
20% w/v polyethylene glycol 8000
|
Resolution 2.35 Å
R-free 0.238
|
|
7CCC
The structure of the actin filament uncapping complex mediated by twinfilin
Deposited 2020-06-16
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 5
PDB declaration: pentameric
|
Chain A
1–377（377 aa）
Chain E
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
CA CALCIUM ION × 2
ADP ADENOSINE-5'-DIPHOSPHATE × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；12% PEG 8000, 10% glycerol, 500 mM potassium chloride
|
Resolution 3.20 Å
R-free 0.238
|
|
7NXV
Crystal structure of the complex of DNase I/G-actin/PPP1R15A_582-621
Deposited 2021-03-19
|
Different oligomeric state
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Other combination
Heteromer；Protein × 3
PDB declaration: trimeric
|
Chain A
3–377（375 aa）
|
Not recorded
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
CA CALCIUM ION × 3
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 5；293 K；10% PEG4000, 0.1M Acetate
|
Resolution 2.55 Å
R-free 0.248
|
|
7NXV
Crystal structure of the complex of DNase I/G-actin/PPP1R15A_582-621
Deposited 2021-03-19
|
Different oligomeric state
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Other combination
Heteromer；Protein × 3
PDB declaration: trimeric
|
Chain D
3–377（375 aa）
|
Not recorded
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
CA CALCIUM ION × 3
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 5；293 K；10% PEG4000, 0.1M Acetate
|
Resolution 2.55 Å
R-free 0.248
|
|
7NZM
Cryo-EM structure of pre-dephosphorylation complex of phosphorylated eIF2alpha with trapped holophosphatase (PP1A_D64A/PPP1R15A/G-actin/DNase I)
Deposited 2021-03-24
|
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Insufficient information
Heteromer；Protein × 5
PDB declaration: pentameric
|
Chain A
3–377（375 aa）
|
Not recorded
|
MN MANGANESE (II) ION × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；0.22mM Triton X-100 was added into the solution before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.96 Å
|
|
7P1G
Structure of the P. aeruginosa ExoY-F-actin complex
Deposited 2021-07-01
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Insufficient information
Heteromer；Protein × 15
PDB declaration: pentadecameric
|
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
|
Not recorded
|
MG MAGNESIUM ION × 10
GH3 3'-DEOXY-GUANOSINE-5'-TRIPHOSPHATE × 5
ADP ADENOSINE-5'-DIPHOSPHATE × 5
PO4 PHOSPHATE ION × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å
|
|
7PLT
Cryo-EM structure of the actomyosin-V complex in the rigor state (central 1er)
Deposited 2021-09-01
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 4
PDB declaration: tetrameric
|
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 1
MG MAGNESIUM ION × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.30 Å
|
|
7PLU
Cryo-EM structure of the actomyosin-V complex in the rigor state (central 3er/2er)
Deposited 2021-09-01
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 10
PDB declaration: decameric
|
Chain C
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 3
MG MAGNESIUM ION × 3
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.20 Å
|
|
7PLV
Cryo-EM structure of the actomyosin-V complex in the rigor state (central 1er, class 1)
Deposited 2021-09-01
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 4
PDB declaration: tetrameric
|
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 1
MG MAGNESIUM ION × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.50 Å
|
|
7PLW
Cryo-EM structure of the actomyosin-V complex in the rigor state (central 1er, class 2)
Deposited 2021-09-01
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 4
PDB declaration: tetrameric
|
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 1
MG MAGNESIUM ION × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.50 Å
|
|
7PLX
Cryo-EM structure of the actomyosin-V complex in the rigor state (central 1er, class 4)
Deposited 2021-09-01
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 4
PDB declaration: tetrameric
|
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 1
MG MAGNESIUM ION × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.60 Å
|
|
7PLY
Cryo-EM structure of the actomyosin-V complex in the rigor state (central 1er, young JASP-stabilized F-actin)
Deposited 2021-09-01
|
Different construct
Different mutation/modification
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 3
PDB declaration: trimeric
|
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 1
PO4 PHOSPHATE ION × 1
MG MAGNESIUM ION × 1
9UE Jasplakinolide × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.20 Å
|
|
7PLZ
Cryo-EM structure of the actomyosin-V complex in the rigor state (central 3er/2er, young JASP-stabilized F-actin)
Deposited 2021-09-01
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 7
PDB declaration: heptameric
|
Chain C
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 3
PO4 PHOSPHATE ION × 3
MG MAGNESIUM ION × 3
9UE Jasplakinolide × 3
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.20 Å
|
|
7PM0
Cryo-EM structure of the actomyosin-V complex in the rigor state (central 1er, young JASP-stabilized F-actin, class 1)
Deposited 2021-09-01
|
Different construct
Different mutation/modification
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 3
PDB declaration: trimeric
|
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 1
PO4 PHOSPHATE ION × 1
MG MAGNESIUM ION × 1
9UE Jasplakinolide × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.60 Å
|
|
7PM1
Cryo-EM structure of the actomyosin-V complex in the rigor state (central 1er, young JASP-stabilized F-actin, class 2)
Deposited 2021-09-01
|
Different construct
Different mutation/modification
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 3
PDB declaration: trimeric
|
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 1
PO4 PHOSPHATE ION × 1
MG MAGNESIUM ION × 1
9UE Jasplakinolide × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.50 Å
|
|
7PM2
Cryo-EM structure of the actomyosin-V complex in the rigor state (central 1er, young JASP-stabilized F-actin, class 4)
Deposited 2021-09-01
|
Different construct
Different mutation/modification
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 3
PDB declaration: trimeric
|
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 1
PO4 PHOSPHATE ION × 1
MG MAGNESIUM ION × 1
9UE Jasplakinolide × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.60 Å
|
|
7PM3
Cryo-EM structure of young JASP-stabilized F-actin (central 3er)
Deposited 2021-09-01
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 3
PDB declaration: trimeric
|
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 3
PO4 PHOSPHATE ION × 3
MG MAGNESIUM ION × 3
9UE Jasplakinolide × 3
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å
|
|
7PM5
Cryo-EM structure of the actomyosin-V complex in the strong-ADP state (central 1er)
Deposited 2021-09-02
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 4
PDB declaration: tetrameric
|
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
MG MAGNESIUM ION × 2
ADP ADENOSINE-5'-DIPHOSPHATE × 2
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.10 Å
|
|
7PM6
Cryo-EM structure of the actomyosin-V complex in the strong-ADP state (central 3er/2er)
Deposited 2021-09-02
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 10
PDB declaration: decameric
|
Chain C
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
MG MAGNESIUM ION × 5
ADP ADENOSINE-5'-DIPHOSPHATE × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.00 Å
|
|
7PM7
Cryo-EM structure of the actomyosin-V complex in the strong-ADP state (central 1er, class 2)
Deposited 2021-09-02
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 4
PDB declaration: tetrameric
|
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
MG MAGNESIUM ION × 2
ADP ADENOSINE-5'-DIPHOSPHATE × 2
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.50 Å
|
|
7PM8
Cryo-EM structure of the actomyosin-V complex in the strong-ADP state (central 1er, class 3)
Deposited 2021-09-02
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 4
PDB declaration: tetrameric
|
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
MG MAGNESIUM ION × 2
ADP ADENOSINE-5'-DIPHOSPHATE × 2
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.50 Å
|
|
7PM9
Cryo-EM structure of the actomyosin-V complex in the strong-ADP state (central 1er, class 4)
Deposited 2021-09-02
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 4
PDB declaration: tetrameric
|
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
MG MAGNESIUM ION × 2
ADP ADENOSINE-5'-DIPHOSPHATE × 2
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.70 Å
|
|
7PMA
Cryo-EM structure of the actomyosin-V complex in the strong-ADP state (central 1er, class 5)
Deposited 2021-09-02
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 4
PDB declaration: tetrameric
|
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
MG MAGNESIUM ION × 2
ADP ADENOSINE-5'-DIPHOSPHATE × 2
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.60 Å
|
|
7PMB
Cryo-EM structure of the actomyosin-V complex in the strong-ADP state (central 1er, class 6)
Deposited 2021-09-02
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 4
PDB declaration: tetrameric
|
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
MG MAGNESIUM ION × 2
ADP ADENOSINE-5'-DIPHOSPHATE × 2
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.60 Å
|
|
7PMC
Cryo-EM structure of the actomyosin-V complex in the strong-ADP state (central 1er, class 7)
Deposited 2021-09-02
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 4
PDB declaration: tetrameric
|
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
MG MAGNESIUM ION × 2
ADP ADENOSINE-5'-DIPHOSPHATE × 2
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration
|
Resolution 3.70 Å
|
|
7PMD
Cryo-EM structure of the actomyosin-V complex in the post-rigor transition state (AppNHp, central 1er)
Deposited 2021-09-02
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 4
PDB declaration: tetrameric
|
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1
MG MAGNESIUM ION × 3
ADP ADENOSINE-5'-DIPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration, two data sets combined
|
Resolution 2.90 Å
|
|
7PME
Cryo-EM structure of the actomyosin-V complex in the post-rigor transition state (AppNHp, central 3er/2er)
Deposited 2021-09-02
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 10
PDB declaration: decameric
|
Chain C
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2
MG MAGNESIUM ION × 7
ADP ADENOSINE-5'-DIPHOSPHATE × 3
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration, two data sets combined
|
Resolution 2.90 Å
|
|
7PMF
Cryo-EM structure of the actomyosin-V complex in the post-rigor transition state (AppNHp, central 1er, class 1)
Deposited 2021-09-02
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 4
PDB declaration: tetrameric
|
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1
MG MAGNESIUM ION × 3
ADP ADENOSINE-5'-DIPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration, two data sets combined
|
Resolution 3.40 Å
|
|
7PMG
Cryo-EM structure of the actomyosin-V complex in the post-rigor transition state (AppNHp, central 1er, class 3)
Deposited 2021-09-02
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 4
PDB declaration: tetrameric
|
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1
MG MAGNESIUM ION × 3
ADP ADENOSINE-5'-DIPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration, two data sets combined
|
Resolution 3.30 Å
|
|
7PMH
Cryo-EM structure of the actomyosin-V complex in the post-rigor transition state (AppNHp, central 1er, class 4)
Deposited 2021-09-02
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 4
PDB declaration: tetrameric
|
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1
MG MAGNESIUM ION × 3
ADP ADENOSINE-5'-DIPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration, two data sets combined
|
Resolution 3.40 Å
|
|
7PMI
Cryo-EM structure of the actomyosin-V complex in the post-rigor transition state (AppNHp, central 1er, class 5)
Deposited 2021-09-02
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 4
PDB declaration: tetrameric
|
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1
MG MAGNESIUM ION × 3
ADP ADENOSINE-5'-DIPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration, two data sets combined
|
Resolution 3.30 Å
|
|
7PMJ
Cryo-EM structure of the actomyosin-V complex in the post-rigor transition state (AppNHp, central 1er, class 6)
Deposited 2021-09-02
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 4
PDB declaration: tetrameric
|
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1
MG MAGNESIUM ION × 3
ADP ADENOSINE-5'-DIPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration, two data sets combined
|
Resolution 3.40 Å
|
|
7PML
Cryo-EM structure of the actomyosin-V complex in the post-rigor transition state (AppNHp, central 1er, class 8)
Deposited 2021-09-02
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 4
PDB declaration: tetrameric
|
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1
MG MAGNESIUM ION × 3
ADP ADENOSINE-5'-DIPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；On grid decoration, two data sets combined
|
Resolution 3.30 Å
|
|
7T5Q
Cryo-EM Structure of a Transition State of Arp2/3 Complex Activation
Deposited 2021-12-13
|
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Insufficient information
Heteromer；Protein × 10
PDB declaration: decameric
|
Chain H
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
MG MAGNESIUM ION × 3
ATP ADENOSINE-5'-TRIPHOSPHATE × 2
ADP ADENOSINE-5'-DIPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE；Grids were manually blotted for 3 seconds with Whatman 41 filter paper and manually plunged using a Leica EM CPC manual plunger.
|
Resolution 3.40 Å
|
|
7TPT
Single-particle Cryo-EM structure of Arp2/3 complex at branched-actin junction.
Deposited 2022-01-26
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 36
PDB declaration: 36-meric
|
Chain H
1–377（377 aa）
Chain I
1–377（377 aa）
Chain J
1–377（377 aa）
Chain K
1–377（377 aa）
Chain L
1–377（377 aa）
Chain M
1–377（377 aa）
Chain N
1–377（377 aa）
Chain O
1–377（377 aa）
Chain P
1–377（377 aa）
Chain Q
1–377（377 aa）
Chain R
1–377（377 aa）
Chain S
1–377（377 aa）
Chain T
1–377（377 aa）
Chain U
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
MG MAGNESIUM ION × 16
ADP ADENOSINE-5'-DIPHOSPHATE × 16
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å
|
|
7U8K
Magic Angle Spinning NMR Structure of Human Cofilin-2 Assembled on Actin Filaments
Deposited 2022-03-08
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 18
PDB declaration: octadecameric
|
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
Chain F
3–377（375 aa）
Chain G
3–377（375 aa）
Chain H
3–377（375 aa）
Chain I
3–377（375 aa）
Chain J
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
No recorded non-water small molecule
|
SOLID-STATE NMR
NMR measurement conditions
pH 6.6；273 K；Ionic strength (raw mmCIF value) 50；Pressure 1
NMR sample composition
20.8 % w/w [U-13C; U-15N] human cofilin-2, 79.2 % w/w F-actin, Solid | Solid
NMR sample composition
20.8 % w/w [1,6-13C]-glucose, U-15N human cofilin-2, 79.2 % w/w F-actin, Solid | Solid
NMR sample composition
20.8 % w/w [2-13C]-glucose, U-15N human cofilin-2, 79.2 % w/w F-actin, Solid | Solid
|
Resolution not provided
|
|
7UTI
ALTERNATIVE MODELING OF TROPOMYOSIN IN HUMAN CARDIAC THIN FILAMENT IN THE CALCIUM BOUND STATE
Deposited 2022-04-27
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 32
PDB declaration: 32-meric
|
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
Chain H
1–377（377 aa）
Chain I
1–377（377 aa）
Chain J
1–377（377 aa）
Chain K
1–377（377 aa）
Chain L
1–377（377 aa）
Chain M
1–377（377 aa）
Chain N
1–377（377 aa）
Chain O
1–377（377 aa）
Chain P
1–377（377 aa）
Chain Q
1–377（377 aa）
Chain R
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 16
MG MAGNESIUM ION × 16
CA CALCIUM ION × 6
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.80 Å
|
|
7UTJ
Cryogenic electron microscopy 3D map of F-actin bound by human dimeric alpha-catenin
Deposited 2022-04-27
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 12
PDB declaration: dodecameric
|
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 6
MG MAGNESIUM ION × 6
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.77 Å
|
|
7UTL
ALTERNATIVE MODELING OF TROPOMYOSIN IN HUMAN CARDIAC THIN FILAMENT IN THE CALCIUM FREE STATE
Deposited 2022-04-27
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 34
PDB declaration: 34-meric
|
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
Chain H
1–377（377 aa）
Chain I
1–377（377 aa）
Chain J
1–377（377 aa）
Chain K
1–377（377 aa）
Chain L
1–377（377 aa）
Chain M
1–377（377 aa）
Chain N
1–377（377 aa）
Chain O
1–377（377 aa）
Chain P
1–377（377 aa）
Chain Q
1–377（377 aa）
Chain R
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 18
MG MAGNESIUM ION × 18
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.60 Å
|
|
7UUW
Cryogenic electron microscopy 3D map of F-actin bound by the Actin Binding Domain of alpha-catenin ortholog, HMP1
Deposited 2022-04-29
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 12
PDB declaration: dodecameric
|
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 6
MG MAGNESIUM ION × 6
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.36 Å
|
|
7UXF
Cryogenic electron microscopy 3D map of F-actin
Deposited 2022-05-05
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 6
PDB declaration: hexameric
|
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 6
MG MAGNESIUM ION × 6
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å
|
|
7WHF
Heimdallarchaeota gelsolin (2DGel) bound to rabbit actin
Deposited 2021-12-30
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain A
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
CA CALCIUM ION × 9
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；297 K；0.4 mM Heimdallarchaeota 2DGel
0.4 mM rabbit actin
1 mM CaCl2
0.1 M HEPES pH 7.0
10% w/v polyethylene glycol 6000
|
Resolution 2.10 Å
R-free 0.216
|
|
7WHF
Heimdallarchaeota gelsolin (2DGel) bound to rabbit actin
Deposited 2021-12-30
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain B
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
CA CALCIUM ION × 9
GOL GLYCEROL × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；297 K；0.4 mM Heimdallarchaeota 2DGel
0.4 mM rabbit actin
1 mM CaCl2
0.1 M HEPES pH 7.0
10% w/v polyethylene glycol 6000
|
Resolution 2.10 Å
R-free 0.216
|
|
7WHG
Lokiarchaeota gelsolin (2DGel) bound to two molecules of rabbit actin
Deposited 2021-12-30
|
Different construct
Different mutation/modification
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 3
PDB declaration: trimeric
|
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 2
CA CALCIUM ION × 9
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；0.4 mM Loki2DGel
0.4 mM rabbit actin
0.1 M Tris-HCl, pH 7.0
0.2 M magnesium chloride hexahydrate
10% w/v polyethylene glycol 8000
1 mM CaCl2
|
Resolution 3.25 Å
R-free 0.232
|
|
7Z7H
Structure of P. luminescens TccC3-F-actin complex
Deposited 2022-03-15
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Insufficient information
Heteromer；Protein × 6
PDB declaration: hexameric
|
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
MG MAGNESIUM ION × 5
APR ADENOSINE-5-DIPHOSPHORIBOSE × 1
NCA NICOTINAMIDE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å
|
|
7Z7I
Structure of ADP-ribosylated F-actin
Deposited 2022-03-15
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 5
PDB declaration: pentameric
|
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
MG MAGNESIUM ION × 5
APR ADENOSINE-5-DIPHOSPHORIBOSE × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å
|
|
8A2R
Cryo-EM structure of F-actin in the Mg2+-ADP-BeF3- nucleotide state.
Deposited 2022-06-06
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 5
PDB declaration: pentameric
|
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
MG MAGNESIUM ION × 5
BEF BERYLLIUM TRIFLUORIDE ION × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；F-buffer:
5 mM Tris pH 7.5, 100 mM KCl, 2 mM MgCl2, 2 mM NaN3, 1 mM DTT, 0.75 mM BeF2, 5 mM NaF.
cryo-EM vitrification conditions
Cryogen ETHANE；The Vitrobot was operated at 13 degrees celsius and the samples were blotted for 9 seconds with a blot force of -25.
|
Resolution 2.17 Å
|
|
8A2S
Cryo-EM structure of F-actin in the Mg2+-ADP-Pi nucleotide state.
Deposited 2022-06-06
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 5
PDB declaration: pentameric
|
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
MG MAGNESIUM ION × 5
PO4 PHOSPHATE ION × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；F-phosphate buffer:
5 mM Tris, 100 mM KCl, 2 mM MgCl2, 2 mM NaN3, 1 mM DTT, 50 mM potassium phosphate pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE；The Vitrobot was operated at 13 degrees celsius and the samples were blotted for 9 seconds with a blot force of -25.
|
Resolution 2.22 Å
|
|
8A2T
Cryo-EM structure of F-actin in the Mg2+-ADP nucleotide state.
Deposited 2022-06-06
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 5
PDB declaration: pentameric
|
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
MG MAGNESIUM ION × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；F-buffer:
5 mM Tris pH 7.5, 100 mM KCl, 2 mM MgCl2, 2 mM NaN3, 1 mM DTT
cryo-EM vitrification conditions
Cryogen ETHANE；The Vitrobot was operated at 13 degrees celsius and the samples were blotted for 9 seconds with a blot force of -25.
|
Resolution 2.24 Å
|
|
8A2U
Cryo-EM structure of F-actin in the Ca2+-ADP-BeF3- nucleotide state.
Deposited 2022-06-06
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 5
PDB declaration: pentameric
|
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
CA CALCIUM ION × 5
BEF BERYLLIUM TRIFLUORIDE ION × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；F-buffer:
5 mM Tris pH 7.5, 100 mM KCl, 2 mM CaCl2, 2 mM NaN3, 1 mM DTT, 0.75 mM BeF2, 5 mM NaF.
cryo-EM vitrification conditions
Cryogen ETHANE；The Vitrobot was operated at 13 degrees celsius and the samples were blotted for 9 seconds with a blot force of -25.
|
Resolution 2.21 Å
|
|
8A2Y
Cryo-EM structure of F-actin in the Ca2+-ADP-Pi nucleotide state.
Deposited 2022-06-06
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 5
PDB declaration: pentameric
|
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
CA CALCIUM ION × 5
PO4 PHOSPHATE ION × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；F-phosphate buffer:
5 mM Tris, 100 mM KCl, 2 mM CaCl2, 2 mM NaN3, 1 mM DTT, 50 mM potassium phosphate pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE；The Vitrobot was operated at 13 degrees celsius and the samples were blotted for 9 seconds with a blot force of -25.
|
Resolution 2.15 Å
|
|
8A2Z
Cryo-EM structure of F-actin in the Ca2+-ADP nucleotide state.
Deposited 2022-06-06
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 5
PDB declaration: pentameric
|
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
CA CALCIUM ION × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；F-buffer:
5 mM Tris pH 7.5, 100 mM KCl, 2 mM CaCl2, 2 mM NaN3, 1 mM DTT
cryo-EM vitrification conditions
Cryogen ETHANE；The Vitrobot was operated at 13 degrees celsius and the samples were blotted for 9 seconds with a blot force of -25.
|
Resolution 2.15 Å
|
|
8BJH
chimera of the inactive ExoY Nucleotidyl Cyclase domain from Vibrio nigripulchritudo MARTX toxin, with the double mutation K3528M and K3535I, fused to a proline-Rich-Domain (PRD) and profilin, bound to Latrunculin B-ADP-Mg-actin
Deposited 2022-11-04
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Insufficient information
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain A
3–377（375 aa）
|
Not recorded
|
ADP ADENOSINE-5'-DIPHOSPHATE × 1
MG MAGNESIUM ION × 1
LAB LATRUNCULIN B × 1
TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 2
GOL GLYCEROL × 3
PEO HYDROGEN PEROXIDE × 8
SO4 SULFATE ION × 2
PEG DI(HYDROXYETHYL)ETHER × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；30% PEG4000 0.2M LISO4 0.1M TRIS PH8.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 1.69 Å
R-free 0.212
|
|
8BJI
chimera of ExoY Nucleotidyl Cyclase domain from Vibrio nigripulchritudo fused to a proline-Rich-Domain (PRD) and profilin, bound to ADP-Mg-actin and a sulfate ion
Deposited 2022-11-04
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Insufficient information
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain A
3–377（375 aa）
|
Not recorded
|
ADP ADENOSINE-5'-DIPHOSPHATE × 1
MG MAGNESIUM ION × 1
TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 2
GOL GLYCEROL × 4
PEG DI(HYDROXYETHYL)ETHER × 2
PEO HYDROGEN PEROXIDE × 14
SO4 SULFATE ION × 3
|
X-RAY DIFFRACTION
X-ray crystallization conditions
EVAPORATION；pH 8.5；293 K；30% peg 4000, 0.2 M Lithium Sulfate (LiSO4), 0.1 M TrisHCl pH8.5
|
Resolution 1.75 Å
R-free 0.212
|
|
8BJJ
ExoY Nucleotidyl Cyclase domain from Vibrio nigripulchritudo MARTX toxin, bound to ATP-Mg-actin, human profilin 1 and a sulfate ion
Deposited 2022-11-04
|
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 3
PDB declaration: trimeric
|
Chain A
3–377（375 aa）
|
Not recorded
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 1
LAB LATRUNCULIN B × 1
TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 2
SO4 SULFATE ION × 4
PEO HYDROGEN PEROXIDE × 2
PG4 TETRAETHYLENE GLYCOL × 1
GOL GLYCEROL × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；291 K；30% peg3000
0.3M LiSO4
0.1M Tris pH8.5
3% Dioxane
|
Resolution 1.70 Å
R-free 0.195
|
|
8BO1
ExoY Nucleotidyl Cyclase domain from Vibrio nigripulchritudo MARTX toxin, bound to Latrunculin-B-ATP-Mg-actin, and 3'-DEOXYADENOSINE-5'-TRIPHOSPHATE and 2 Mg ions
Deposited 2022-11-14
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain A
3–377（375 aa）
|
Not recorded
|
LAB LATRUNCULIN B × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 3
SO4 SULFATE ION × 1
3AT 3'-DEOXYADENOSINE-5'-TRIPHOSPHATE × 1
MN MANGANESE (II) ION × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；291 K；26 % peg 3350, 26 % Glycerol, 30 mM LiSO4, 0.1M TrisHCl pH8.5, 3 % Dioxane,
|
Resolution 2.50 Å
R-free 0.231
|
|
8BO1
ExoY Nucleotidyl Cyclase domain from Vibrio nigripulchritudo MARTX toxin, bound to Latrunculin-B-ATP-Mg-actin, and 3'-DEOXYADENOSINE-5'-TRIPHOSPHATE and 2 Mg ions
Deposited 2022-11-14
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain C
3–377（375 aa）
|
Not recorded
|
LAB LATRUNCULIN B × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 3
SO4 SULFATE ION × 2
3AT 3'-DEOXYADENOSINE-5'-TRIPHOSPHATE × 1
MN MANGANESE (II) ION × 6
AZI AZIDE ION × 6
CL CHLORIDE ION × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；291 K；26 % peg 3350, 26 % Glycerol, 30 mM LiSO4, 0.1M TrisHCl pH8.5, 3 % Dioxane,
|
Resolution 2.50 Å
R-free 0.231
|
|
8BR0
ExoY Nucleotidyl Cyclase domain from Vibrio nigripulchritudo MARTX toxin (residue Q3455 to L3863) in complex with 3'deoxyCTP and two manganese cations bound to Latrunculin-B-ADP-Mn-actin
Deposited 2022-11-22
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Insufficient information
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain A
3–377（375 aa）
|
Not recorded
|
LAB LATRUNCULIN B × 1
ADP ADENOSINE-5'-DIPHOSPHATE × 1
MN MANGANESE (II) ION × 3
CH1 3'-DEOXY-CYTIDINE-5'-TRIPHOSPHATE × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；10mg/mL binary complex in the presence of
15.2 mM 3primedCTP1.6 mM ADP, 20 mM MgCl2, 0.2 mM Latrunculin B, 23 mM KCl, 70 mM LiCl, 8 mM HEPES pH 8.5, 4 mM TCEP and mixed with 17 % PEG4000, 17 % Glycerol, 0.01 M Li2SO4, 0.1 M Tris pH 8.5, 5 mM MgCl2, 15 mM MnCl2, 1% 1-Butyl-2,3-dimethylimidazolium tetrafluoroborate (ionic liquid 18 from the Ionic Liquid Screen (Hampton Research)) in a 1:1.2 v/v hanging drop
|
Resolution 2.22 Å
R-free 0.243
|
|
8BR0
ExoY Nucleotidyl Cyclase domain from Vibrio nigripulchritudo MARTX toxin (residue Q3455 to L3863) in complex with 3'deoxyCTP and two manganese cations bound to Latrunculin-B-ADP-Mn-actin
Deposited 2022-11-22
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Insufficient information
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain C
3–377（375 aa）
|
Not recorded
|
LAB LATRUNCULIN B × 1
ADP ADENOSINE-5'-DIPHOSPHATE × 1
MN MANGANESE (II) ION × 3
CH1 3'-DEOXY-CYTIDINE-5'-TRIPHOSPHATE × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；10mg/mL binary complex in the presence of
15.2 mM 3primedCTP1.6 mM ADP, 20 mM MgCl2, 0.2 mM Latrunculin B, 23 mM KCl, 70 mM LiCl, 8 mM HEPES pH 8.5, 4 mM TCEP and mixed with 17 % PEG4000, 17 % Glycerol, 0.01 M Li2SO4, 0.1 M Tris pH 8.5, 5 mM MgCl2, 15 mM MnCl2, 1% 1-Butyl-2,3-dimethylimidazolium tetrafluoroborate (ionic liquid 18 from the Ionic Liquid Screen (Hampton Research)) in a 1:1.2 v/v hanging drop
|
Resolution 2.22 Å
R-free 0.243
|
|
8BR1
ExoY Nucleotidyl Cyclase domain from Vibrio nigripulchritudo MARTX toxin, bound to Latrunculin-B-ATP-Mg-actin, and 3'-DEOXYADENOSINE-5'-TRIPHOSPHATE and 2 Mg ions
Deposited 2022-11-22
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain A
3–377（375 aa）
|
Not recorded
|
LAB LATRUNCULIN B × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 3
3AT 3'-DEOXYADENOSINE-5'-TRIPHOSPHATE × 1
SO4 SULFATE ION × 1
GOL GLYCEROL × 1
PEO HYDROGEN PEROXIDE × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；26 % peg 3350, 26 % Glycerol, 30 mM LiSO4,
0.1M TrisHCl pH8.5,
3 % Dioxane,
|
Resolution 2.04 Å
R-free 0.222
|
|
8BR1
ExoY Nucleotidyl Cyclase domain from Vibrio nigripulchritudo MARTX toxin, bound to Latrunculin-B-ATP-Mg-actin, and 3'-DEOXYADENOSINE-5'-TRIPHOSPHATE and 2 Mg ions
Deposited 2022-11-22
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain C
3–377（375 aa）
|
Not recorded
|
LAB LATRUNCULIN B × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 3
3AT 3'-DEOXYADENOSINE-5'-TRIPHOSPHATE × 1
SO4 SULFATE ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；26 % peg 3350, 26 % Glycerol, 30 mM LiSO4,
0.1M TrisHCl pH8.5,
3 % Dioxane,
|
Resolution 2.04 Å
R-free 0.222
|
|
8DMX
Cryo-EM structure of skeletal muscle alpha-actin
Deposited 2022-07-08
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 4
PDB declaration: tetrameric
|
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
MG MAGNESIUM ION × 4
ADP ADENOSINE-5'-DIPHOSPHATE × 4
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.37 Å
|
|
8F8P
Cryo-EM structure of F-actin in the ADP state
Deposited 2022-11-22
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 5
PDB declaration: pentameric
|
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
MG MAGNESIUM ION × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Blot force 0
Blot time 2.5 s
|
Resolution 2.26 Å
|
|
8F8Q
Cryo-EM structure of the CapZ-capped barbed end of F-actin
Deposited 2022-11-22
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 8
PDB declaration: octameric
|
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 6
MG MAGNESIUM ION × 6
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Blot force 0
Blot time 2.5 s
|
Resolution 2.79 Å
|
|
8F8R
Cryo-EM structure of the free barbed end of F-actin
Deposited 2022-11-22
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 7
PDB declaration: heptameric
|
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 7
MG MAGNESIUM ION × 7
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Blot force 0
Blot time 2.5 s
|
Resolution 3.30 Å
|
|
8F8S
Cryo-EM structure of the free pointed end of F-actin
Deposited 2022-11-22
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 7
PDB declaration: heptameric
|
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 7
MG MAGNESIUM ION × 7
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Blot force 0
Blot time 2.5 s
|
Resolution 2.84 Å
|
|
8F8T
Cryo-EM structure of the Tropomodulin-capped pointed end of F-actin
Deposited 2022-11-22
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 8
PDB declaration: octameric
|
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
Chain F
1–377（377 aa）
Chain G
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 7
MG MAGNESIUM ION × 7
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Blot force 0
Blot time 2.5 s
|
Resolution 3.26 Å
|
|
8JO3
Cryo-EM structure of a Legionella effector complexed with actin and AMP
Deposited 2023-06-07
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain B
1–377（377 aa）
|
Not recorded
|
AMP ADENOSINE MONOPHOSPHATE × 1
CA CALCIUM ION × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.66 Å
|
|
8JO4
Cryo-EM structure of a Legionella effector complexed with actin and ATP
Deposited 2023-06-07
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain B
1–377（377 aa）
|
Not recorded
|
ATP ADENOSINE-5'-TRIPHOSPHATE × 2
MG MAGNESIUM ION × 1
CA CALCIUM ION × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.04 Å
|
|
8OF8
Cryo-EM structure of actomyosin-5a-S1 with the full-length lever (nucleotide free)
Deposited 2023-03-14
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 10
PDB declaration: decameric
|
Chain J
3–377（375 aa）
Chain K
3–377（375 aa）
Chain L
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.50 Å
|
|
8PVX
Structure of the Lifeact13-F-actin complex
Deposited 2023-07-18
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 10
PDB declaration: decameric
|
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
Chain D
1–377（377 aa）
Chain E
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
MG MAGNESIUM ION × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.55 Å
|
|
8R9V
CryoEM structure of the primed actomyosin-5a complex
Deposited 2023-11-30
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 4
PDB declaration: tetrameric
|
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
PO4 PHOSPHATE ION × 1
MG MAGNESIUM ION × 4
ADP ADENOSINE-5'-DIPHOSPHATE × 4
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE；F-actin was mixed with myosin-5a (S1 1 IQ motif, residues 1-797, S217A, DDEK 594-597 deletion) that had been pre-incubated with ATP, and vitrified at 10 ms post-mixing
|
Resolution 4.40 Å
|
|
8RBF
CryoEM structure of the post-powerstroke actomyosin-5a complex
Deposited 2023-12-04
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 4
PDB declaration: tetrameric
|
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
MG MAGNESIUM ION × 4
ADP ADENOSINE-5'-DIPHOSPHATE × 4
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE；F-actin was mixed with myosin-5a (S1 1 IQ motif, residues 1-797, S217A, DDEK 594-597 deletion) that had been pre-incubated with ATP, and vitrified at 120 ms post-mixing
|
Resolution 4.20 Å
|
|
8RU0
Structure of the undecorated barbed end of F-actin.
Deposited 2024-01-29
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 4
PDB declaration: tetrameric
|
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 3
MG MAGNESIUM ION × 4
PO4 PHOSPHATE ION × 3
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.1；12 mM HEPES pH 7.1, 100 mM KCl, 2.1 mM MgCl2, 1 mM EGTA, 1 mM TCEP, 0.2 mM ATP
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE；3 seconds, force 0.
|
Resolution 3.08 Å
|
|
8RV2
Structure of the formin INF2 bound to the barbed end of F-actin.
Deposited 2024-01-31
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 6
PDB declaration: hexameric
|
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 3
MG MAGNESIUM ION × 4
PO4 PHOSPHATE ION × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.1；12 mM HEPES pH 7.1, 100 mM KCl, 2.1 mM MgCl2, 1 mM EGTA, 1 mM TCEP, 0.2 mM ATP
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE；3 seconds, force 0.
|
Resolution 3.41 Å
|
|
8UEE
Atomic structure of Salmonella SipA/F-actin complex by cryo-EM
Deposited 2023-10-01
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 11
PDB declaration: undecameric
|
Chain F
3–377（375 aa）
Chain H
3–377（375 aa）
Chain I
3–377（375 aa）
Chain J
3–377（375 aa）
Chain K
3–377（375 aa）
Chain L
3–377（375 aa）
Chain M
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 7
MG MAGNESIUM ION × 7
PO4 PHOSPHATE ION × 7
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8；Buffer composition:
25 mM TRIS-H-Cl pH 8.0
2 mM DTT
cryo-EM vitrification conditions
Cryogen ETHANE；3 uL of sample was applied on Lacey grid, then sample was blotted for 3 seconds and plunge-froze in liquid ethane
|
Resolution 3.20 Å
|
|
8UXW
Arp2/3 branch junction complex, ADP state
Deposited 2023-11-11
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 15
PDB declaration: pentadecameric
|
Chain H
1–377（377 aa）
Chain I
1–377（377 aa）
Chain M
1–377（377 aa）
Chain N
1–377（377 aa）
Chain O
1–377（377 aa）
Chain P
1–377（377 aa）
Chain Q
1–377（377 aa）
Chain R
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 9
MG MAGNESIUM ION × 10
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；The samples were incubated on the grid for 50 s and the extra solution was blotted using two Vitrobot filter papers (0.55/20 mm, Grade 595, Ted Pella) for 4 s at 0 blot force. The grids were plunged into liquid ethane at ~180 degrees C with a wait time of 0.5 s.
|
Resolution 2.70 Å
|
|
8UXX
Arp2/3 branch junction complex, BeFx state
Deposited 2023-11-11
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 15
PDB declaration: pentadecameric
|
Chain H
1–377（377 aa）
Chain I
1–377（377 aa）
Chain M
1–377（377 aa）
Chain N
1–377（377 aa）
Chain O
1–377（377 aa）
Chain P
1–377（377 aa）
Chain Q
1–377（377 aa）
Chain R
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 9
MG MAGNESIUM ION × 10
BEF BERYLLIUM TRIFLUORIDE ION × 7
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；The samples were incubated on the grid for 50 s and the extra solution was blotted using two Vitrobot filter papers (0.55/20 mm, Grade 595, Ted Pella) for 4 s at 0 blot force. The grids were plunged into liquid ethane at ~180 degrees C with a wait time of 0.5 s.
|
Resolution 3.20 Å
|
|
8UZ0
Straight actin filament from Arp2/3 branch junction sample (ADP)
Deposited 2023-11-14
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 9
PDB declaration: nonameric
|
Chain J
6–377（372 aa）
Chain K
6–377（372 aa）
Chain L
6–377（372 aa）
Chain M
6–377（372 aa）
Chain N
6–377（372 aa）
Chain O
6–377（372 aa）
Chain P
6–377（372 aa）
Chain Q
6–377（372 aa）
Chain R
6–377（372 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 9
MG MAGNESIUM ION × 9
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；The samples were incubated on the grid for 50 s and the extra solution was blotted using two Vitrobot filter papers (0.55/20 mm, Grade 595, Ted Pella) for 4 s at 0 blot force. The grids were plunged into liquid ethane at ~180 degrees C with a wait time of 0.5 s.
|
Resolution 2.80 Å
|
|
8UZ1
Straight actin filament from Arp2/3 branch junction sample (ADP-BeFx)
Deposited 2023-11-14
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 9
PDB declaration: nonameric
|
Chain J
6–377（372 aa）
Chain K
6–377（372 aa）
Chain L
6–377（372 aa）
Chain M
6–377（372 aa）
Chain N
6–377（372 aa）
Chain O
6–377（372 aa）
Chain P
6–377（372 aa）
Chain Q
6–377（372 aa）
Chain R
6–377（372 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 9
MG MAGNESIUM ION × 9
BEF BERYLLIUM TRIFLUORIDE ION × 9
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；The samples were incubated on the grid for 50 s and the extra solution was blotted using two Vitrobot filter papers (0.55/20 mm, Grade 595, Ted Pella) for 4 s at 0 blot force. The grids were plunged into liquid ethane at ~180 degrees C with a wait time of 0.5 s.
|
Resolution 3.60 Å
|
|
8VIZ
Structure of full-length gelsolin bound to the barbed end of F-actin
Deposited 2024-01-05
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 7
PDB declaration: heptameric
|
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
Chain F
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
CA CALCIUM ION × 14
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.63 Å
|
|
8VKH
Structure of gelsolin domains G1G3 bound to the barbed end of F-actin
Deposited 2024-01-09
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 8
PDB declaration: octameric
|
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
Chain F
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 4
CA CALCIUM ION × 14
ATP ADENOSINE-5'-TRIPHOSPHATE × 2
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.63 Å
|
|
8W36
rabbit actin in the absence of potassium
Deposited 2024-02-21
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 5
PDB declaration: pentameric
|
Chain A
7–377（371 aa）
Chain B
7–377（371 aa）
Chain C
7–377（371 aa）
Chain D
7–377（371 aa）
Chain E
7–377（371 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
MG MAGNESIUM ION × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.27 Å
|
|
8XDL
F-actin-END
Deposited 2023-12-11
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 3
PDB declaration: trimeric
|
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 3
MG MAGNESIUM ION × 3
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.44 Å
|
|
8XDM
F-actin-MAD
Deposited 2023-12-11
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 3
PDB declaration: trimeric
|
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 3
MG MAGNESIUM ION × 3
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.45 Å
|
|
8YAE
Cryo-ET structure of huntingtin actin complex
Deposited 2024-02-09
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 14
PDB declaration: tetradecameric
|
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
Chain F
3–377（375 aa）
Chain G
3–377（375 aa）
Chain H
3–377（375 aa）
Chain I
3–377（375 aa）
Chain J
3–377（375 aa）
Chain K
3–377（375 aa）
Chain L
3–377（375 aa）
Chain M
3–377（375 aa）
Chain N
3–377（375 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 10.08 Å
|
|
8YAO
Cryo-ET structure of huntingtin actin dimer complex
Deposited 2024-02-09
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 28
PDB declaration: 28-meric
|
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
Chain F
3–377（375 aa）
Chain G
3–377（375 aa）
Chain H
3–377（375 aa）
Chain I
3–377（375 aa）
Chain J
3–377（375 aa）
Chain K
3–377（375 aa）
Chain L
3–377（375 aa）
Chain M
3–377（375 aa）
Chain N
3–377（375 aa）
Chain b
3–377（375 aa）
Chain c
3–377（375 aa）
Chain d
3–377（375 aa）
Chain e
3–377（375 aa）
Chain f
3–377（375 aa）
Chain g
3–377（375 aa）
Chain h
3–377（375 aa）
Chain i
3–377（375 aa）
Chain j
3–377（375 aa）
Chain k
3–377（375 aa）
Chain l
3–377（375 aa）
Chain m
3–377（375 aa）
Chain n
3–377（375 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 20.80 Å
|
|
9AZ4
INF2 at the Barbed End of F-Actin
Deposited 2024-03-10
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 8
PDB declaration: octameric
|
Chain A
7–377（371 aa）
Chain B
7–377（371 aa）
Chain C
7–377（371 aa）
Chain D
7–377（371 aa）
Chain E
7–377（371 aa）
Chain F
7–377（371 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 6
MG MAGNESIUM ION × 6
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.37 Å
|
|
9AZ6
F-actin-Talin(R13-DD) complex
Deposited 2024-03-10
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 13
PDB declaration: tridecameric
|
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
Chain E
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
MG MAGNESIUM ION × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；3.0 ul sample, blotted for 5 s from both sides with filter paper Whatman No.1
|
Resolution 2.98 Å
|
|
9AZP
INF2 at the Barbed End of F-Actin with Incoming Profilin-Actin
Deposited 2024-03-11
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 10
PDB declaration: decameric
|
Chain A
7–377（371 aa）
Chain B
7–377（371 aa）
Chain C
7–377（371 aa）
Chain D
7–377（371 aa）
Chain E
7–377（371 aa）
Chain F
7–377（371 aa）
Chain I
7–377（371 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 6
MG MAGNESIUM ION × 7
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.79 Å
|
|
9AZQ
INF2 at the Barbed End of F-Actin with Incoming Actin
Deposited 2024-03-11
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 9
PDB declaration: nonameric
|
Chain A
7–377（371 aa）
Chain B
7–377（371 aa）
Chain C
7–377（371 aa）
Chain D
7–377（371 aa）
Chain E
7–377（371 aa）
Chain F
7–377（371 aa）
Chain I
7–377（371 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 7
MG MAGNESIUM ION × 7
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.82 Å
|
|
9B03
INF2 in the Middle of F-Actin (Up state)
Deposited 2024-03-11
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 8
PDB declaration: octameric
|
Chain A
7–377（371 aa）
Chain B
7–377（371 aa）
Chain C
7–377（371 aa）
Chain D
7–377（371 aa）
Chain E
7–377（371 aa）
Chain F
7–377（371 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 6
MG MAGNESIUM ION × 6
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.95 Å
|
|
9B0K
INF2 in the Middle of F-Actin (Down state)
Deposited 2024-03-12
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 8
PDB declaration: octameric
|
Chain A
7–377（371 aa）
Chain B
7–377（371 aa）
Chain C
7–377（371 aa）
Chain D
7–377（371 aa）
Chain E
7–377（371 aa）
Chain F
7–377（371 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 6
MG MAGNESIUM ION × 6
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.03 Å
|
|
9B27
Dia1 at the Barbed End of F-Actin
Deposited 2024-03-14
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 8
PDB declaration: octameric
|
Chain A
7–377（371 aa）
Chain B
7–377（371 aa）
Chain C
7–377（371 aa）
Chain D
7–377（371 aa）
Chain E
7–377（371 aa）
Chain F
7–377（371 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 6
MG MAGNESIUM ION × 6
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.51 Å
|
|
9B3D
mDia1 in the middle of F-actin
Deposited 2024-03-19
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 8
PDB declaration: octameric
|
Chain A
7–377（371 aa）
Chain B
7–377（371 aa）
Chain C
7–377（371 aa）
Chain D
7–377（371 aa）
Chain E
7–377（371 aa）
Chain F
7–377（371 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 6
MG MAGNESIUM ION × 6
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8；20mM HEPES, 50mM NaCL, 1mM EDTA, 1mM DTT, 0.05% Thesit
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.41 Å
|
|
9CFU
Cryo-EM structure of myosin-1c bound to F-actin in the ADP-A state
Deposited 2024-06-27
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 5
PDB declaration: pentameric
|
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
|
Not recorded
|
ADP ADENOSINE-5'-DIPHOSPHATE × 4
MG MAGNESIUM ION × 4
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å
|
|
9CFV
Cryo-EM structure of delta-NTR myosin-1c bound to F-actin
Deposited 2024-06-27
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 5
PDB declaration: pentameric
|
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
|
Not recorded
|
ADP ADENOSINE-5'-DIPHOSPHATE × 3
MG MAGNESIUM ION × 3
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å
|
|
9CFW
Cryo-EM structure of myosin-1c bound to F-actin in the ADP-B state
Deposited 2024-06-27
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 5
PDB declaration: pentameric
|
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
|
Not recorded
|
ADP ADENOSINE-5'-DIPHOSPHATE × 4
MG MAGNESIUM ION × 3
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å
|
|
9CFX
Cryo-EM structure of myosin-1c bound to F-actin in the Rigor state
Deposited 2024-06-27
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 5
PDB declaration: pentameric
|
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
|
Not recorded
|
ADP ADENOSINE-5'-DIPHOSPHATE × 3
MG MAGNESIUM ION × 3
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å
|
|
9DFS
Structure of novel Myo7a-N isoform (ADP-bound) expressed in sensory hair cells (head domain + first two IQ domains), bound to F-actin
Deposited 2024-08-30
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 4
PDB declaration: tetrameric
|
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 4
MG MAGNESIUM ION × 4
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å
|
|
9DFV
Structure of canonical Myo7a-C isoform (ADP-bound) expressed in sensory hair cells (head domain + first two IQ domains), bound to F-actin
Deposited 2024-08-30
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 4
PDB declaration: tetrameric
|
Chain A
1–377（377 aa）
Chain B
1–377（377 aa）
Chain C
1–377（377 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 4
MG MAGNESIUM ION × 4
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å
|
|
9FJO
Structure of the undecorated pointed end of F-actin
Deposited 2024-05-31
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 4
PDB declaration: tetrameric
|
Chain A
3–377（375 aa）
Chain B
3–377（375 aa）
Chain C
3–377（375 aa）
Chain D
3–377（375 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 4
MG MAGNESIUM ION × 4
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.1；12 mM HEPES pH 7.1, 100 mM KCl, 2.1 mM MgCl2, 1 mM EGTA, 1 mM TCEP, 0.2 mM ATP
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE；3 seconds, force 0.
|
Resolution 3.05 Å
|
|
9GOB
Structure of the F-tractin-F-actin complex
Deposited 2024-09-05
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 6
PDB declaration: hexameric
|
Chain A
4–377（374 aa）
Chain B
4–377（374 aa）
Chain C
4–377（374 aa）
Chain D
4–377（374 aa）
Chain E
4–377（374 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
MG MAGNESIUM ION × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.20 Å
|
|
9HM9
Structure of the optimized F-tractin in complex with F-actin
Deposited 2024-12-07
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 6
PDB declaration: hexameric
|
Chain A
4–377（374 aa）
Chain B
4–377（374 aa）
Chain C
4–377（374 aa）
Chain D
4–377（374 aa）
Chain E
4–377（374 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
MG MAGNESIUM ION × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.40 Å
|
|
9KBX
CryoEM structure of F-actin bound with GAS2-CH3 domain.
Deposited 2024-10-31
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 6
PDB declaration: hexameric
|
Chain H
7–376（370 aa）
Chain J
7–376（370 aa）
Chain K
7–376（370 aa）
Chain L
7–376（370 aa）
|
Not recorded
|
ADP ADENOSINE-5'-DIPHOSPHATE × 4
MG MAGNESIUM ION × 4
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8；50 mM Tris-HCl, pH 8.0, 200 mM KCl, 2 mM MgCl2, 1 mM EGTA, 4 mM DTT.
cryo-EM vitrification conditions
Cryogen ETHANE；The grid was blotted for 4 s at force 10 and plunged into ethane immediately.
|
Resolution 2.80 Å
|
|
9NB9
Viral protein DP71L in complex with phosphorylated eIF2alpha (NTD) and protein phosphatase 1A (D64A), stabilized by G-actin/DNAseI
Deposited 2025-02-13
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 5
PDB declaration: pentameric
|
Chain D
1–377（377 aa）
|
Not recorded
|
MN MANGANESE (II) ION × 1
CA CALCIUM ION × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；3.5 uL volume, -5 blot force, 1.5 blot time
|
Resolution 3.03 Å
|
|
9P3D
cryo-EM structure of Vibrio effector VopV fragment bound to skeletal alpha F-actin
Deposited 2025-06-13
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 22
PDB declaration: 22-meric
|
Chain Q
6–377（372 aa）
Chain R
6–377（372 aa）
Chain S
6–377（372 aa）
Chain T
6–377（372 aa）
Chain U
6–377（372 aa）
Chain V
6–377（372 aa）
Chain W
6–377（372 aa）
Chain X
6–377（372 aa）
Chain Y
6–377（372 aa）
Chain Z
6–377（372 aa）
Chain a
6–377（372 aa）
|
Not recorded
|
MG MAGNESIUM ION × 11
ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 11
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å
|
|
9Q7K
Pointed end of Cofilin-2 Bound F-actin
Deposited 2025-08-24
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 10
PDB declaration: decameric
|
Chain A
2–377（376 aa）
Chain B
2–377（376 aa）
Chain C
2–377（376 aa）
Chain D
2–377（376 aa）
Chain E
2–377（376 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
MG MAGNESIUM ION × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.13 Å
|
|
9Q7L
Barbed end of F-actin and cofilin on sides
Deposited 2025-08-24
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 8
PDB declaration: octameric
|
Chain A
2–377（376 aa）
Chain B
2–377（376 aa）
Chain C
2–377（376 aa）
Chain D
2–377（376 aa）
Chain E
2–377（376 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
MG MAGNESIUM ION × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.68 Å
|
|
9Q7M
Barbed end of cofilin actin, cofilin on second-to-last barbed end subunit
Deposited 2025-08-24
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 9
PDB declaration: nonameric
|
Chain A
2–377（376 aa）
Chain B
2–377（376 aa）
Chain C
2–377（376 aa）
Chain D
2–377（376 aa）
Chain E
2–377（376 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
MG MAGNESIUM ION × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å
|
|
9Q7N
Cofilin barbed end, cofilin on the two barbed end subunits
Deposited 2025-08-24
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 10
PDB declaration: decameric
|
Chain A
2–377（376 aa）
Chain B
2–377（376 aa）
Chain C
2–377（376 aa）
Chain D
2–377（376 aa）
Chain E
2–377（376 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
MG MAGNESIUM ION × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.48 Å
|
|
9Q7O
One CAP-1 Bound to the Pointed End of F-actin
Deposited 2025-08-24
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 6
PDB declaration: hexameric
|
Chain A
2–377（376 aa）
Chain B
2–377（376 aa）
Chain C
2–377（376 aa）
Chain D
2–377（376 aa）
Chain E
2–377（376 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
MG MAGNESIUM ION × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.02 Å
|
|
9UG2
Severed and capped actin fragment by two G1G3 domains of gelsolin
Deposited 2025-04-11
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 9
PDB declaration: nonameric
|
Chain C
7–376（370 aa）
Chain D
7–376（370 aa）
Chain E
7–376（370 aa）
Chain F
7–376（370 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
CA CALCIUM ION × 8
ADP ADENOSINE-5'-DIPHOSPHATE × 4
MG MAGNESIUM ION × 5
PO4 PHOSPHATE ION × 2
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.89 Å
|
|
9XYE
Two CAP-1 Bound to the Pointed End of F-actin
Deposited 2025-08-25
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 7
PDB declaration: heptameric
|
Chain A
2–377（376 aa）
Chain B
2–377（376 aa）
Chain C
2–377（376 aa）
Chain D
2–377（376 aa）
Chain E
2–377（376 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
MG MAGNESIUM ION × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.18 Å
|
|
9Y52
One CAP-1 Bound to the Pointed End of Cofilin F-actin
Deposited 2025-09-04
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 11
PDB declaration: undecameric
|
Chain A
2–377（376 aa）
Chain B
2–377（376 aa）
Chain C
2–377（376 aa）
Chain D
2–377（376 aa）
Chain E
2–377（376 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
MG MAGNESIUM ION × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.46 Å
|
|
9Y9J
Two CAP-1 Bound to the Pointed End of Cofilin F-actin
Deposited 2025-09-14
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 12
PDB declaration: dodecameric
|
Chain A
2–377（376 aa）
Chain B
2–377（376 aa）
Chain C
2–377（376 aa）
Chain D
2–377（376 aa）
Chain E
2–377（376 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
MG MAGNESIUM ION × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.58 Å
|
|
9Y9L
CP at the barbed end with one cofilin on second-to-last subunit
Deposited 2025-09-14
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 11
PDB declaration: 11-meric
|
Chain A
2–377（376 aa）
Chain B
2–377（376 aa）
Chain C
2–377（376 aa）
Chain D
2–377（376 aa）
Chain E
2–377（376 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
MG MAGNESIUM ION × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.06 Å
|
|
9Y9M
Capping protein bound to the barbed end of cofilactin
Deposited 2025-09-14
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 10
PDB declaration: decameric
|
Chain A
2–377（376 aa）
Chain B
2–377（376 aa）
Chain C
2–377（376 aa）
Chain D
2–377（376 aa）
Chain E
2–377（376 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
MG MAGNESIUM ION × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.06 Å
|
|
9Y9P
Cofilactin filament
Deposited 2025-09-14
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 10
PDB declaration: decameric
|
Chain A
2–377（376 aa）
Chain B
2–377（376 aa）
Chain C
2–377（376 aa）
Chain D
2–377（376 aa）
Chain E
2–377（376 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
MG MAGNESIUM ION × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.06 Å
|
|
9YIM
Capping protein bound to the barbed end of F-actin
Deposited 2025-10-01
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 7
PDB declaration: heptameric
|
Chain A
7–377（371 aa）
Chain B
7–377（371 aa）
Chain C
7–377（371 aa）
Chain D
7–377（371 aa）
Chain E
7–377（371 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
Non-standard monomer：Yes (specific site not provided by mmCIF)
|
ADP ADENOSINE-5'-DIPHOSPHATE × 5
MG MAGNESIUM ION × 5
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.62 Å
|
