Complement C3
Homo sapiens
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Protein heterocomplex Heteromer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count | Chain A； UniProt 994–1288 | Fragment：unp residues 994-1288 Mutation：C1010A, C144A, I332G | Integrin alpha-M × 1 （P11215） NI NICKEL (II) ION × 2 | X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；PEG 3350, pH 7, vapor diffusion, hanging drop, temperature 298K | Resolution 2.80 Å R-free 0.242 |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 4M76 | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1C3D X-RAY CRYSTAL STRUCTURE OF C3D: A C3 FRAGMENT AND LIGAND FOR COMPLEMENT RECEPTOR 2 Deposited 1998-05-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
996–1287（292 aa）
|
Mutation：C17A | GOL GLYCEROL × 5 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.5；PROTEIN WAS CRYSTALLIZED FROM 12% PEG 20K, 100 MM MES BUFFER, PH 6.5, 10 MM DTT
|
Resolution 1.80 Å R-free 0.230 |
| 1GHQ CR2-C3D COMPLEX STRUCTURE Deposited 2001-01-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
996–1300（305 aa）
Fragment：FRAGMENT OF ALPHA CHAIN
|
Mutation：C17A | ZN ZINC ION × 2 NDG 2-acetamido-2-deoxy-alpha-D-glucopyranose × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6；pH 6.0
|
Resolution 2.04 Å R-free 0.239 |
| 1W2S Solution structure of CR2 SCR 1-2 in its complex with C3d by X-ray scattering Deposited 2004-07-08 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
996–1287（292 aa）
Chain A
1288–1299（12 aa）
|
Not recorded | No recorded non-water small molecule | SOLUTION SCATTERING mmCIF provides none of the parsed conditions | Resolution not provided |
| 2A73 Human Complement Component C3 Deposited 2005-07-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
23–665（643 aa）
Fragment：residue 1-645
Chain B
673–1663（991 aa）
Fragment：residue 650-1663
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.2；293 K；PEG 550 MME, sodium acetate, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 3.30 Å R-free 0.289 |
| 2A74 Human Complement Component C3c Deposited 2005-07-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
23–665（643 aa）
Fragment：residues 23-665
Chain B
749–936（188 aa）
Fragment：residues 749-936
Chain C
1321–1663（343 aa）
Fragment：esidues 1321-1663
|
Not recorded | GOL GLYCEROL × 7 NO3 NITRATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；PEG3000, lithium nitrate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.40 Å R-free 0.275 |
| 2A74 Human Complement Component C3c Deposited 2005-07-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Other combination Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain D
23–665（643 aa）
Fragment：residues 23-665
Chain E
749–936（188 aa）
Fragment：residues 749-936
Chain F
1321–1663（343 aa）
Fragment：esidues 1321-1663
|
Not recorded | GOL GLYCEROL × 6 NO3 NITRATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；PEG3000, lithium nitrate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.40 Å R-free 0.275 |
| 2GOX Crystal structure of Efb-C / C3d Complex Deposited 2006-04-14 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
996–1287（292 aa）
Fragment：Fragment of alpha chain: Residues 996-1287
|
Mutation：C1010A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.4；293 K；60% Tacsimate pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
|
Resolution 2.20 Å R-free 0.231 |
| 2GOX Crystal structure of Efb-C / C3d Complex Deposited 2006-04-14 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
996–1287（292 aa）
Fragment：Fragment of alpha chain: Residues 996-1287
|
Mutation：C1010A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.4；293 K；60% Tacsimate pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
|
Resolution 2.20 Å R-free 0.231 |
| 2I07 Human Complement Component C3b Deposited 2006-08-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
23–667（645 aa）
Chain B
749–1663（915 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.8；303 K；PEG-MME 2000, sodium acetate,
Bis-Tris propane, taurine, pH 7.8, VAPOR DIFFUSION, SITTING DROP, temperature 303K
|
Resolution 4.00 Å R-free 0.323 |
| 2ICE CRIg bound to C3c Deposited 2006-09-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain A
23–664（642 aa）
Chain B
749–954（206 aa）
Chain C
1321–1663（343 aa）
|
Not recorded | CA CALCIUM ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 3.10 Å R-free 0.295 |
| 2ICE CRIg bound to C3c Deposited 2006-09-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain D
23–664（642 aa）
Chain E
749–954（206 aa）
Chain F
1321–1663（343 aa）
|
Not recorded | CA CALCIUM ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 3.10 Å R-free 0.295 |
| 2ICF CRIg bound to C3b Deposited 2006-09-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
23–664（642 aa）
Chain B
749–1663（915 aa）
|
Not recorded | CA CALCIUM ION × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
EVAPORATION；pH 6.5；293 K；pH 6.5, EVAPORATION, temperature 293K
|
Resolution 4.10 Å R-free 0.330 |
| 2NOJ Crystal structure of Ehp / C3d complex Deposited 2006-10-25 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
996–1287（292 aa）
Fragment：Residues 996-1287
|
Mutation：C1010A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8.2；293 K；0.2M LiSO4, 25% PEG 3350, 0.1M Tris-HCl pH 8.2, additive: CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.70 Å R-free 0.284 |
| 2NOJ Crystal structure of Ehp / C3d complex Deposited 2006-10-25 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
996–1287（292 aa）
Fragment：Residues 996-1287
|
Mutation：C1010A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8.2；293 K；0.2M LiSO4, 25% PEG 3350, 0.1M Tris-HCl pH 8.2, additive: CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.70 Å R-free 0.284 |
| 2NOJ Crystal structure of Ehp / C3d complex Deposited 2006-10-25 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain E
996–1287（292 aa）
Fragment：Residues 996-1287
|
Mutation：C1010A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8.2；293 K；0.2M LiSO4, 25% PEG 3350, 0.1M Tris-HCl pH 8.2, additive: CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.70 Å R-free 0.284 |
| 2NOJ Crystal structure of Ehp / C3d complex Deposited 2006-10-25 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain G
996–1287（292 aa）
Fragment：Residues 996-1287
|
Mutation：C1010A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8.2；293 K；0.2M LiSO4, 25% PEG 3350, 0.1M Tris-HCl pH 8.2, additive: CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.70 Å R-free 0.284 |
| 2QKI Human C3c in complex with the inhibitor compstatin Deposited 2007-07-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain A
23–665（643 aa）
Fragment：residues 23-665
Chain B
749–936（188 aa）
Fragment：residues 749-936
Chain C
1321–1663（343 aa）
Fragment：residues 1321-1663
|
Not recorded | K POTASSIUM ION × 2 BR BROMIDE ION × 12 GOL GLYCEROL × 7 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；PEG-MME 2000, KBr, Tris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.40 Å R-free 0.281 |
| 2QKI Human C3c in complex with the inhibitor compstatin Deposited 2007-07-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Other combination Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain D
23–665（643 aa）
Fragment：residues 23-665
Chain E
749–936（188 aa）
Fragment：residues 749-936
Chain F
1321–1663（343 aa）
Fragment：residues 1321-1663
|
Not recorded | K POTASSIUM ION × 3 BR BROMIDE ION × 20 GOL GLYCEROL × 11 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；PEG-MME 2000, KBr, Tris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.40 Å R-free 0.281 |
| 2WII Complement C3b in complex with factor H domains 1-4 Deposited 2009-05-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
23–667（645 aa）
Fragment：RESIDUES 23-667
Chain B
749–1663（915 aa）
Fragment：RESIDUES 749-1663
|
Not recorded | CA CALCIUM ION × 1 GOL GLYCEROL × 17 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.1；7.0% (W/V) PEG 3,350, 70 MM AMMONIUM ACETATE, PH 7.1
|
Resolution 2.70 Å R-free 0.252 |
| 2WII Complement C3b in complex with factor H domains 1-4 Deposited 2009-05-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Other combination Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
23–667（645 aa）
Fragment：RESIDUES 23-667
|
Not recorded | CA CALCIUM ION × 1 GOL GLYCEROL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.1；7.0% (W/V) PEG 3,350, 70 MM AMMONIUM ACETATE, PH 7.1
|
Resolution 2.70 Å R-free 0.252 |
| 2WII Complement C3b in complex with factor H domains 1-4 Deposited 2009-05-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
749–1663（915 aa）
Fragment：RESIDUES 749-1663
|
Not recorded | GOL GLYCEROL × 13 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.1；7.0% (W/V) PEG 3,350, 70 MM AMMONIUM ACETATE, PH 7.1
|
Resolution 2.70 Å R-free 0.252 |
| 2WIN C3 convertase (C3bBb) stabilized by SCIN Deposited 2009-05-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
23–667（645 aa）
Fragment：COMPLEMENT C3B BETA CHAIN, RESIDUES 23-667
Chain B
749–1663（915 aa）
Fragment：;COMPLEMENT C3B ALPHA' CHAIN, RESIDUES 749-1663
;
Chain G
23–667（645 aa）
Fragment：COMPLEMENT C3B BETA CHAIN, RESIDUES 23-667
Chain H
749–1663（915 aa）
Fragment：;COMPLEMENT C3B ALPHA' CHAIN, RESIDUES 749-1663
;
|
Not recorded | MAN alpha-D-mannopyranose × 1 BMA beta-D-mannopyranose × 1 MG MAGNESIUM ION × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.5；75 MM SODIUM/POTASSIUM TARTRATE, 8.0% PEG 3350, 50 MM BIS-TRIS PROPANE, PH 6.5
|
Resolution 3.90 Å R-free 0.268 |
| 2WIN C3 convertase (C3bBb) stabilized by SCIN Deposited 2009-05-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Other combination Heteromer；Protein × 8 PDB declaration: octameric |
Chain C
23–667（645 aa）
Fragment：COMPLEMENT C3B BETA CHAIN, RESIDUES 23-667
Chain D
749–1663（915 aa）
Fragment：;COMPLEMENT C3B ALPHA' CHAIN, RESIDUES 749-1663
;
Chain E
23–667（645 aa）
Fragment：COMPLEMENT C3B BETA CHAIN, RESIDUES 23-667
Chain F
749–1663（915 aa）
Fragment：;COMPLEMENT C3B ALPHA' CHAIN, RESIDUES 749-1663
;
|
Not recorded | BMA beta-D-mannopyranose × 1 MG MAGNESIUM ION × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.5；75 MM SODIUM/POTASSIUM TARTRATE, 8.0% PEG 3350, 50 MM BIS-TRIS PROPANE, PH 6.5
|
Resolution 3.90 Å R-free 0.268 |
| 2WY7 Staphylococcus aureus complement subversion protein Sbi-IV in complex with complement fragment C3d revealing an alternative binding mode Deposited 2009-11-13 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
996–1303（308 aa）
Fragment：C3D, RESIDUES 996-1303
|
Mutation：YES | GOL GLYCEROL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；100MM TRIS PH8.0, 200MM NACL, 20%(W/V) PEG 4000
|
Resolution 1.70 Å R-free 0.205 |
| 2WY8 Staphylococcus aureus complement subversion protein Sbi-IV in complex with complement fragment C3d Deposited 2009-11-13 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
996–1303（308 aa）
Fragment：C3D, RESIDUES 996-1303
|
Mutation：YES | GOL GLYCEROL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；100MM TRIS PH8.0, 200MM NACL, 20%(W/V) PEG 4000
|
Resolution 1.70 Å R-free 0.205 |
| 2XQW Structure of Factor H domains 19-20 in complex with complement C3d Deposited 2010-09-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
996–1287（292 aa）
Fragment：THIOESTER DOMAIN, RESIDUES 996-1287
Chain B
996–1287（292 aa）
Fragment：THIOESTER DOMAIN, RESIDUES 996-1287
|
Mutation：YES Mutation：YES | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5；295 K；12-18% PEG 4000, 0.1 M HEPES, PH 7.5, AT 22 DEGREES C
|
Resolution 2.31 Å R-free 0.242 |
| 2XQW Structure of Factor H domains 19-20 in complex with complement C3d Deposited 2010-09-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
996–1287（292 aa）
Fragment：THIOESTER DOMAIN, RESIDUES 996-1287
|
Mutation：YES | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5；295 K；12-18% PEG 4000, 0.1 M HEPES, PH 7.5, AT 22 DEGREES C
|
Resolution 2.31 Å R-free 0.242 |
| 2XQW Structure of Factor H domains 19-20 in complex with complement C3d Deposited 2010-09-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
996–1287（292 aa）
Fragment：THIOESTER DOMAIN, RESIDUES 996-1287
|
Mutation：YES | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5；295 K；12-18% PEG 4000, 0.1 M HEPES, PH 7.5, AT 22 DEGREES C
|
Resolution 2.31 Å R-free 0.242 |
| 2XWB Crystal Structure of Complement C3b in complex with Factors B and D Deposited 2010-11-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain A
23–664（642 aa）
Fragment：RESIDUES 23-664
Chain B
752–1663（912 aa）
Fragment：RESIDUES 752-1663
|
Not recorded | MG MAGNESIUM ION × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.7；PH 7.7
|
Resolution 3.49 Å R-free 0.244 |
| 2XWB Crystal Structure of Complement C3b in complex with Factors B and D Deposited 2010-11-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Other combination Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
23–664（642 aa）
Fragment：RESIDUES 23-664
Chain D
752–1663（912 aa）
Fragment：RESIDUES 752-1663
|
Not recorded | MG MAGNESIUM ION × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.7；PH 7.7
|
Resolution 3.49 Å R-free 0.244 |
| 2XWB Crystal Structure of Complement C3b in complex with Factors B and D Deposited 2010-11-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Other combination Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
23–664（642 aa）
Fragment：RESIDUES 23-664
Chain D
752–1663（912 aa）
Fragment：RESIDUES 752-1663
|
Not recorded | MG MAGNESIUM ION × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.7；PH 7.7
|
Resolution 3.49 Å R-free 0.244 |
| 2XWB Crystal Structure of Complement C3b in complex with Factors B and D Deposited 2010-11-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Other combination Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain A
23–664（642 aa）
Fragment：RESIDUES 23-664
Chain B
752–1663（912 aa）
Fragment：RESIDUES 752-1663
|
Not recorded | MG MAGNESIUM ION × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.7；PH 7.7
|
Resolution 3.49 Å R-free 0.244 |
| 2XWJ Crystal Structure of Complement C3b in Complex with Factor B Deposited 2010-11-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
23–667（645 aa）
Fragment：RESIDUES 23-667
Chain B
749–1663（915 aa）
Fragment：RESIDUES 749-1663
|
Mutation：YES | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 NI NICKEL (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 4；pH 4.0
|
Resolution 4.00 Å R-free 0.281 |
| 2XWJ Crystal Structure of Complement C3b in Complex with Factor B Deposited 2010-11-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain C
23–667（645 aa）
Fragment：RESIDUES 23-667
Chain D
749–1663（915 aa）
Fragment：RESIDUES 749-1663
|
Mutation：YES | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 NI NICKEL (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 4；pH 4.0
|
Resolution 4.00 Å R-free 0.281 |
| 2XWJ Crystal Structure of Complement C3b in Complex with Factor B Deposited 2010-11-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain G
23–667（645 aa）
Fragment：RESIDUES 23-667
Chain H
749–1663（915 aa）
Fragment：RESIDUES 749-1663
|
Mutation：YES | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 NI NICKEL (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 4；pH 4.0
|
Resolution 4.00 Å R-free 0.281 |
| 2XWJ Crystal Structure of Complement C3b in Complex with Factor B Deposited 2010-11-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain E
23–667（645 aa）
Fragment：RESIDUES 23-667
Chain F
749–1663（915 aa）
Fragment：RESIDUES 749-1663
|
Mutation：YES | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 NI NICKEL (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 4；pH 4.0
|
Resolution 4.00 Å R-free 0.281 |
| 30JE Herpes simplex virus 2 delta28-73 glycoprotein C ectodomain in complex with C3b Deposited 2026-04-29 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain B
23–667（645 aa）
Chain C
749–1663（915 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 3D5R Crystal Structure of Efb-C (N138A) / C3d Complex Deposited 2008-05-16 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
996–1287（292 aa）
Fragment：Complement C3d fragment, UNP residues 996-1287
|
Mutation：C1010A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；60% (v/v) tacsimate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.10 Å R-free 0.241 |
| 3D5R Crystal Structure of Efb-C (N138A) / C3d Complex Deposited 2008-05-16 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
996–1287（292 aa）
Fragment：Complement C3d fragment, UNP residues 996-1287
|
Mutation：C1010A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；60% (v/v) tacsimate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.10 Å R-free 0.241 |
| 3D5S Crystal Structure of Efb-C (R131A) / C3d Complex Deposited 2008-05-16 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
996–1287（292 aa）
Fragment：Complement C3d fragment, UNP residues 996-1287
|
Mutation：C1010A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；60% (v/v) tacsimate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.30 Å R-free 0.218 |
| 3D5S Crystal Structure of Efb-C (R131A) / C3d Complex Deposited 2008-05-16 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
996–1287（292 aa）
Fragment：Complement C3d fragment, UNP residues 996-1287
|
Mutation：C1010A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；60% (v/v) tacsimate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.30 Å R-free 0.218 |
| 3G6J C3b in complex with a C3b specific Fab Deposited 2009-02-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain A
23–666（644 aa）
Chain B
749–1663（915 aa）
|
Not recorded | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.8；Crystals of the C3b:S77 complex were obtained within a week at 19\xB0 C
from 4 ml hanging drops, consisting of a 1:1 ratio of protein
solution (10 mg/ml protein in 50 mM NaCl, 25 mM TRIS pH 7.8) to
mother liquor (10% PEG 4000, 0.2 M MgCl2, 100 mM Na-HEPES, pH 7.0),
suspended over mother liquor, VAPOR DIFFUSION, HANGING DROP
|
Resolution 3.10 Å R-free 0.282 |
| 3G6J C3b in complex with a C3b specific Fab Deposited 2009-02-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
23–666（644 aa）
Chain D
749–1663（915 aa）
|
Not recorded | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.8；Crystals of the C3b:S77 complex were obtained within a week at 19\xB0 C
from 4 ml hanging drops, consisting of a 1:1 ratio of protein
solution (10 mg/ml protein in 50 mM NaCl, 25 mM TRIS pH 7.8) to
mother liquor (10% PEG 4000, 0.2 M MgCl2, 100 mM Na-HEPES, pH 7.0),
suspended over mother liquor, VAPOR DIFFUSION, HANGING DROP
|
Resolution 3.10 Å R-free 0.282 |
| 3G6J C3b in complex with a C3b specific Fab Deposited 2009-02-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 5 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
23–666（644 aa）
Chain B
749–1663（915 aa）
|
Not recorded | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.8；Crystals of the C3b:S77 complex were obtained within a week at 19\xB0 C
from 4 ml hanging drops, consisting of a 1:1 ratio of protein
solution (10 mg/ml protein in 50 mM NaCl, 25 mM TRIS pH 7.8) to
mother liquor (10% PEG 4000, 0.2 M MgCl2, 100 mM Na-HEPES, pH 7.0),
suspended over mother liquor, VAPOR DIFFUSION, HANGING DROP
|
Resolution 3.10 Å R-free 0.282 |
| 3G6J C3b in complex with a C3b specific Fab Deposited 2009-02-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 6 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain C
23–666（644 aa）
Chain D
749–1663（915 aa）
|
Not recorded | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.8；Crystals of the C3b:S77 complex were obtained within a week at 19\xB0 C
from 4 ml hanging drops, consisting of a 1:1 ratio of protein
solution (10 mg/ml protein in 50 mM NaCl, 25 mM TRIS pH 7.8) to
mother liquor (10% PEG 4000, 0.2 M MgCl2, 100 mM Na-HEPES, pH 7.0),
suspended over mother liquor, VAPOR DIFFUSION, HANGING DROP
|
Resolution 3.10 Å R-free 0.282 |
| 3L3O Staphylococcal Complement Inhibitor (SCIN) in complex with Human Complement Component C3c Deposited 2009-12-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain A
23–667（645 aa）
Fragment：residues 23-667
Chain B
749–954（206 aa）
Fragment：residues 749-954
Chain C
1321–1663（343 aa）
Fragment：residues 1321-1663
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；Protein solution: 5mg/ml, 0.1M HEPES, 10% PEG 6000, 5% 2-Methyl-2,4-pentanediol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 3.40 Å R-free 0.271 |
| 3L3O Staphylococcal Complement Inhibitor (SCIN) in complex with Human Complement Component C3c Deposited 2009-12-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain D
23–667（645 aa）
Fragment：residues 23-667
Chain E
749–954（206 aa）
Fragment：residues 749-954
Chain F
1321–1663（343 aa）
Fragment：residues 1321-1663
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；Protein solution: 5mg/ml, 0.1M HEPES, 10% PEG 6000, 5% 2-Methyl-2,4-pentanediol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 3.40 Å R-free 0.271 |
| 3L5N Staphylococcal Complement Inhibitor (SCIN) in complex with Human Complement Component C3b Deposited 2009-12-22 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
23–667（645 aa）
Fragment：residues 23-667
Chain B
749–1663（915 aa）
Fragment：residues 749-1663
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 MAN alpha-D-mannopyranose × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；Protein solution 5mg/ml, 0.1M HEPES-NaOH, 30%(v/v) Jeffamine ED-2001-HCl, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 7.54 Å R-free 0.268 |
| 3NMS Staphylococcal Complement Inhibitor (SCIN) in complex with Human Complement C3c Deposited 2010-06-22 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain A
23–667（645 aa）
Chain B
749–954（206 aa）
Chain C
1321–1663（343 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；Protein solution: 5mg/ml, 0.1M HEPES, 10% PEG 6000, 5% 2-Methyl-2,4-pentanediol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 4.10 Å R-free 0.289 |
| 3OED The structure of the complex between complement receptor CR2 and its ligand complement fragment C3d Deposited 2010-08-12 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
996–1303（308 aa）
Fragment：C3 (unp residues 996-1303)
|
Mutation：C1010A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；100 mM HEPES, 20% Poly Ethylene Glycol 6000,
200 mM CaCl2, pH 7.0, VAPOR DIFFUSION, HANGING DROP
|
Resolution 3.16 Å R-free 0.259 |
| 3OED The structure of the complex between complement receptor CR2 and its ligand complement fragment C3d Deposited 2010-08-12 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
996–1303（308 aa）
Fragment：C3 (unp residues 996-1303)
|
Mutation：C1010A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；100 mM HEPES, 20% Poly Ethylene Glycol 6000,
200 mM CaCl2, pH 7.0, VAPOR DIFFUSION, HANGING DROP
|
Resolution 3.16 Å R-free 0.259 |
| 3OHX Molecular Basis for Complement Recognition and Inhibition Determined by Crystallographic Studies of the Staphylococcal Complement Inhibitor (SCIN) Bound to C3c and C3b Deposited 2010-08-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain A
23–667（645 aa）
Fragment：Complement C3 beta chain
Chain B
749–954（206 aa）
Fragment：;Complement C3 alpha' chain fragment 1
;
Chain C
1321–1663（343 aa）
Fragment：;Complement C3 alpha' chain fragment 2
;
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；2.5mg/ml protein, 0.1M HEPES, 10% PEG6000, 5% 2-Methyl-2,4-pentanediol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 3.50 Å R-free 0.294 |
| 3OHX Molecular Basis for Complement Recognition and Inhibition Determined by Crystallographic Studies of the Staphylococcal Complement Inhibitor (SCIN) Bound to C3c and C3b Deposited 2010-08-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain D
23–667（645 aa）
Fragment：Complement C3 beta chain
Chain E
749–954（206 aa）
Fragment：;Complement C3 alpha' chain fragment 1
;
Chain F
1321–1663（343 aa）
Fragment：;Complement C3 alpha' chain fragment 2
;
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；2.5mg/ml protein, 0.1M HEPES, 10% PEG6000, 5% 2-Methyl-2,4-pentanediol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 3.50 Å R-free 0.294 |
| 3OXU Complement components factor H CCP19-20 and C3d in complex Deposited 2010-09-22 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
996–1303（308 aa）
Fragment：UNP residues 996-1303
|
Mutation：C1010A | GOL GLYCEROL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.8；290 K；4% w/v PEG 8000, 0.1 M Tris HCl, pH 8.8, VAPOR DIFFUSION, HANGING DROP, temperature 290K
|
Resolution 2.10 Å R-free 0.206 |
| 3OXU Complement components factor H CCP19-20 and C3d in complex Deposited 2010-09-22 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
996–1303（308 aa）
Fragment：UNP residues 996-1303
|
Mutation：C1010A | GOL GLYCEROL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.8；290 K；4% w/v PEG 8000, 0.1 M Tris HCl, pH 8.8, VAPOR DIFFUSION, HANGING DROP, temperature 290K
|
Resolution 2.10 Å R-free 0.206 |
| 3OXU Complement components factor H CCP19-20 and C3d in complex Deposited 2010-09-22 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
996–1303（308 aa）
Fragment：UNP residues 996-1303
|
Mutation：C1010A | GOL GLYCEROL × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.8；290 K；4% w/v PEG 8000, 0.1 M Tris HCl, pH 8.8, VAPOR DIFFUSION, HANGING DROP, temperature 290K
|
Resolution 2.10 Å R-free 0.206 |
| 3RJ3 Complement components factor H CCP19-20 (S1191L mutant) and C3D in complex Deposited 2011-04-15 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
996–1303（308 aa）
Fragment：UNP residues 996-1303
|
Not recorded | GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；290 K；4% w/v PEG 8000, 0.1 M Tris HCl, pH 8.8, VAPOR DIFFUSION, HANGING DROP, temperature 290K
|
Resolution 2.35 Å R-free 0.232 |
| 3RJ3 Complement components factor H CCP19-20 (S1191L mutant) and C3D in complex Deposited 2011-04-15 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
996–1303（308 aa）
Fragment：UNP residues 996-1303
|
Not recorded | GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；290 K；4% w/v PEG 8000, 0.1 M Tris HCl, pH 8.8, VAPOR DIFFUSION, HANGING DROP, temperature 290K
|
Resolution 2.35 Å R-free 0.232 |
| 3RJ3 Complement components factor H CCP19-20 (S1191L mutant) and C3D in complex Deposited 2011-04-15 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
996–1303（308 aa）
Fragment：UNP residues 996-1303
|
Not recorded | GOL GLYCEROL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；290 K；4% w/v PEG 8000, 0.1 M Tris HCl, pH 8.8, VAPOR DIFFUSION, HANGING DROP, temperature 290K
|
Resolution 2.35 Å R-free 0.232 |
| 3T4A Structure of a truncated form of Staphylococcal Complement Inhibitor B bound to human C3c at 3.4 Angstrom resolution Deposited 2011-07-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain A
23–667（645 aa）
Fragment：C3c beta chain, UNP residues 23-667
Chain B
749–954（206 aa）
Fragment：;C3c alpha' chain fragment 1, UNP residues 749-954
;
Chain C
1321–1663（343 aa）
Fragment：;C3c alpha' chain fragment 2, UNP residues 1321-1663
;
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.5；293 K；5mg/ml protein, 0.1M bis-Tris, 15% w/v PEG 3350, pH 6.5, vapor diffusion, hanging drop, temperature 293K
|
Resolution 3.40 Å R-free 0.270 |
| 3T4A Structure of a truncated form of Staphylococcal Complement Inhibitor B bound to human C3c at 3.4 Angstrom resolution Deposited 2011-07-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain D
23–667（645 aa）
Fragment：C3c beta chain, UNP residues 23-667
Chain E
749–954（206 aa）
Fragment：;C3c alpha' chain fragment 1, UNP residues 749-954
;
Chain F
1321–1663（343 aa）
Fragment：;C3c alpha' chain fragment 2, UNP residues 1321-1663
;
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.5；293 K；5mg/ml protein, 0.1M bis-Tris, 15% w/v PEG 3350, pH 6.5, vapor diffusion, hanging drop, temperature 293K
|
Resolution 3.40 Å R-free 0.270 |
| 4HW5 Crystal Structure of the Human C3a anaphylatoxin Deposited 2012-11-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
672–748（77 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；292 K；2-2.2 M ammonium sulfate with the pH ranging from 6 to 9 , VAPOR DIFFUSION, SITTING DROP, temperature 292K
|
Resolution 2.25 Å R-free 0.243 |
| 4HW5 Crystal Structure of the Human C3a anaphylatoxin Deposited 2012-11-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
672–748（77 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；292 K；2-2.2 M ammonium sulfate with the pH ranging from 6 to 9 , VAPOR DIFFUSION, SITTING DROP, temperature 292K
|
Resolution 2.25 Å R-free 0.243 |
| 4HW5 Crystal Structure of the Human C3a anaphylatoxin Deposited 2012-11-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
672–748（77 aa）
Chain B
672–748（77 aa）
|
Not recorded | SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；292 K；2-2.2 M ammonium sulfate with the pH ranging from 6 to 9 , VAPOR DIFFUSION, SITTING DROP, temperature 292K
|
Resolution 2.25 Å R-free 0.243 |
| 4HWJ Crystal Structure of the Human C3a desArg anaphylatoxin Deposited 2012-11-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
672–747（76 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；292 K；0.1 M Tris pH 7.5-8.5, 25 % w/v PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 292K
|
Resolution 2.60 Å R-free 0.270 |
| 4I6O Crystal structure of chemically synthesized human anaphylatoxin C3a Deposited 2012-11-29 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
672–748（77 aa）
Fragment：C3a anaphylatoxin (UNP residues 672-748)
|
Not recorded | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；277 K；VAPOR DIFFUSION, HANGING DROP, 1 uL 5 mg/mL lyophilized C3a in 2 mM hydrochloric acid + 1 uL reservoir (0.1 M Tris-HCl, 0.2 M ammonium phosphate monobasic, 49-51% v/v MPD, pH 8.5) against 500 uL reservoir, prepared at 277K, incubated at 281K for 96 hours, stored at 277K, rhomboid-shaped crystals appeared after 6 days and grew to a final size of 0.5 x 0.2 x 0.2 mm
|
Resolution 2.14 Å R-free 0.268 |
| 4ONT Ternary host recognition complex of complement factor H, C3d, and sialic acid Deposited 2014-01-29 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
996–1303（308 aa）
Fragment：UNP residues 996-1303
|
Mutation：C17A | GOL GLYCEROL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；293 K；0.1 M Tris-HCl, pH 9.0, 8% w/v PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.15 Å R-free 0.223 |
| 4ONT Ternary host recognition complex of complement factor H, C3d, and sialic acid Deposited 2014-01-29 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Other combination Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
996–1303（308 aa）
Fragment：UNP residues 996-1303
|
Mutation：C17A | GOL GLYCEROL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；293 K；0.1 M Tris-HCl, pH 9.0, 8% w/v PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.15 Å R-free 0.223 |
| 4ONT Ternary host recognition complex of complement factor H, C3d, and sialic acid Deposited 2014-01-29 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Other combination Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
996–1303（308 aa）
Fragment：UNP residues 996-1303
|
Mutation：C17A | GOL GLYCEROL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；293 K；0.1 M Tris-HCl, pH 9.0, 8% w/v PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.15 Å R-free 0.223 |
| 4ZH1 Complement factor H in complex with the GM1 glycan Deposited 2015-04-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
996–1303（308 aa）
|
Not recorded | GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；293 K；0.1 M TRIS-HCL, PH 9.0, 8% W/V PRG 8000
|
Resolution 2.24 Å R-free 0.228 |
| 4ZH1 Complement factor H in complex with the GM1 glycan Deposited 2015-04-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Other combination Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
996–1303（308 aa）
|
Not recorded | GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；293 K；0.1 M TRIS-HCL, PH 9.0, 8% W/V PRG 8000
|
Resolution 2.24 Å R-free 0.228 |
| 4ZH1 Complement factor H in complex with the GM1 glycan Deposited 2015-04-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Other combination Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
996–1303（308 aa）
|
Not recorded | GOL GLYCEROL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；293 K；0.1 M TRIS-HCL, PH 9.0, 8% W/V PRG 8000
|
Resolution 2.24 Å R-free 0.228 |
| 5FO7 Crystal Structure of Human Complement C3b at 2.8 Angstrom resolution Deposited 2015-11-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
23–667（645 aa）
Fragment：RESIDUES 23-667
Chain B
749–1663（915 aa）
Fragment：RESIDUES 749-1663
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
8% PEG 3350 35MM BIS-TRIS PH 5.5
|
Resolution 2.80 Å R-free 0.268 |
| 5FO8 Crystal Structure of Human Complement C3b in Complex with MCP (CCP1-4) Deposited 2015-11-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
23–667（645 aa）
Fragment：UNP RESIDUES 23-667
Chain B
749–1663（915 aa）
Fragment：UNP RESIDUES 749-1663
|
Not recorded | EDO 1,2-ETHANEDIOL × 32 |
X-RAY DIFFRACTION
X-ray crystallization conditions
100MM AMMONIUM CITRATE 7% PEG 3350 5MM GLUTATHIONE 50MM BIS-TRIS PROPANE, PH 6.5
|
Resolution 2.40 Å R-free 0.219 |
| 5FO9 Crystal Structure of Human Complement C3b in Complex with CR1 (CCP15- 17) Deposited 2015-11-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain D
23–667（645 aa）
Fragment：RESIDUES 23-667
Chain E
749–1663（915 aa）
Fragment：CCP DOMAINS, RESIDUES 749-1663
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
8% PEG 3350 35MM BIS-TRIS PH 5.5
|
Resolution 3.30 Å R-free 0.291 |
| 5FO9 Crystal Structure of Human Complement C3b in Complex with CR1 (CCP15- 17) Deposited 2015-11-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
23–667（645 aa）
Fragment：RESIDUES 23-667
Chain B
749–1663（915 aa）
Fragment：CCP DOMAINS, RESIDUES 749-1663
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
8% PEG 3350 35MM BIS-TRIS PH 5.5
|
Resolution 3.30 Å R-free 0.291 |
| 5FOA Crystal Structure of Human Complement C3b in complex with DAF (CCP2-4) Deposited 2015-11-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
23–667（645 aa）
Fragment：UNP RESIDUES 23-667
Chain B
749–1663（915 aa）
Fragment：UNP RESIDUES 749-1663
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
60MM MGCL2 30MM BIS-TRIS PH 5.5 6.5% PEG 3350 3% MESO-ERHYTROL
|
Resolution 4.19 Å R-free 0.307 |
| 5FOA Crystal Structure of Human Complement C3b in complex with DAF (CCP2-4) Deposited 2015-11-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain C
23–667（645 aa）
Fragment：UNP RESIDUES 23-667
Chain D
749–1663（915 aa）
Fragment：UNP RESIDUES 749-1663
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
60MM MGCL2 30MM BIS-TRIS PH 5.5 6.5% PEG 3350 3% MESO-ERHYTROL
|
Resolution 4.19 Å R-free 0.307 |
| 5FOB Crystal Structure of Human Complement C3b in complex with Smallpox Inhibitor of Complement (SPICE) Deposited 2015-11-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
23–667（645 aa）
Fragment：RESIDUES 23-667
Chain B
749–1663（915 aa）
Fragment：CCP DOMAINS, RESIDUES 749-1663
|
Not recorded | CL CHLORIDE ION × 5 GOL GLYCEROL × 24 NA SODIUM ION × 2 IOD IODIDE ION × 7 |
X-RAY DIFFRACTION
X-ray crystallization conditions
75MM AMMONIUM IODIDE, 3.5% PEG 3350
|
Resolution 2.60 Å R-free 0.230 |
| 5NBQ The structure of the tripartite complex between OspE, the C-terminal domains of factor H and C3dg Deposited 2017-03-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
994–1287（294 aa）
|
Mutation：A1153E, C1010A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；20% PEG3350, 0.1 M HEPES pH 7.5, 0.2 M MgCl2 or
24% PEG3350, 0.1 M HEPES pH 7.5, 0.2 M MgCl2 or
16% PEG3350, Tris pH 8.5, 0.2 M ammonium acetate
|
Resolution 3.18 Å R-free 0.261 |
| 5NBQ The structure of the tripartite complex between OspE, the C-terminal domains of factor H and C3dg Deposited 2017-03-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain B
994–1287（294 aa）
|
Mutation：A1153E, C1010A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；20% PEG3350, 0.1 M HEPES pH 7.5, 0.2 M MgCl2 or
24% PEG3350, 0.1 M HEPES pH 7.5, 0.2 M MgCl2 or
16% PEG3350, Tris pH 8.5, 0.2 M ammonium acetate
|
Resolution 3.18 Å R-free 0.261 |
| 5NBQ The structure of the tripartite complex between OspE, the C-terminal domains of factor H and C3dg Deposited 2017-03-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain C
994–1287（294 aa）
|
Mutation：A1153E, C1010A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；20% PEG3350, 0.1 M HEPES pH 7.5, 0.2 M MgCl2 or
24% PEG3350, 0.1 M HEPES pH 7.5, 0.2 M MgCl2 or
16% PEG3350, Tris pH 8.5, 0.2 M ammonium acetate
|
Resolution 3.18 Å R-free 0.261 |
| 5O32 The structure of complement complex Deposited 2017-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
23–667（645 aa）
Fragment：beta chain, UNP residues 23-667
Chain B
749–1663（915 aa）
Fragment：alpha chain, UNP residues 749-1663
Chain E
23–667（645 aa）
Fragment：beta chain, UNP residues 23-667
Chain F
749–1663（915 aa）
Fragment：alpha chain, UNP residues 749-1663
|
Not recorded | CA CALCIUM ION × 6 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 12 MLI MALONATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291.15 K；60mM sodium malonate PH5.0
6% w/v PEG3350
|
Resolution 4.21 Å R-free 0.254 |
| 5O35 Structure of complement proteins complex Deposited 2017-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
23–667（645 aa）
Chain B
749–1663（915 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291.15 K；60mM Ammonium sulfate, 6% w/v PEG3350
|
Resolution 4.20 Å R-free 0.246 |
| 6EHG complement component C3b in complex with a nanobody Deposited 2017-09-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
23–665（643 aa）
Chain B
749–1663（915 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293.15 K；12 mM Sodium Citrate pH 8, 28 mM Sodium Citrate pH 6.0, 12.25 % (w/v) PEG 2000 MME, 70 mM Imidazole pH 7, 60 mM Ammonium acetate, 70 mM Tris pH 8.5, 13.5 % (v/v) and (+/-)-2-Methyl-2,4-pentanediol
|
Resolution 2.65 Å |
| 6RMT Crystal structure of disulphide-linked human C3d dimer Deposited 2019-05-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
996–1303（308 aa）
Chain B
996–1303（308 aa）
|
Not recorded | CL CHLORIDE ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；291 K；100 mM Tris pH 8, 200 mM NaCl, 24% PEG 4000
|
Resolution 2.00 Å R-free 0.200 |
| 6RMU Crystal structure of disulphide-linked human C3d dimer in complex with Staphylococcus aureus complement subversion protein Sbi-IV Deposited 2019-05-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain A
996–1303（308 aa）
Chain B
996–1303（308 aa）
|
Not recorded | PEG DI(HYDROXYETHYL)ETHER × 1 EDO 1,2-ETHANEDIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.07；291 K；0.2 M sodium citrate tribasic dihydrate, 20% PEG 3350.
|
Resolution 2.40 Å R-free 0.211 |
| 6RU5 human complement C3 in complex with the hC3Nb1 nanobody Deposited 2019-05-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
23–667（645 aa）
Chain B
672–1663（992 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；100 mM HEPES pH 7.2, 1.75 % (w/v) PEG8000
|
Resolution 3.90 Å |
| 6RUR Structure of the SCIN stabilized C3bBb convertase bound to properdin Deposited 2019-05-29 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain A
23–667（645 aa）
Chain B
749–1663（915 aa）
Chain G
23–667（645 aa）
Chain H
749–1663（915 aa）
|
Not recorded | MAN alpha-D-mannopyranose × 18 MG MAGNESIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；277 K；50 mM MgAcetate 50 mM Mes 6.5 5 % w/v PEG 10K
|
Resolution 6.00 Å R-free 0.272 |
| 6RUV Structure of the SCIN stabilized C3bBb convertase bound to Properdin and a the non-inhibitory nanobody hFPNb1 Deposited 2019-05-29 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 14 PDB declaration: tetradecameric |
Chain A
23–667（645 aa）
Chain B
749–1663（915 aa）
Chain G
23–667（645 aa）
Chain H
749–1663（915 aa）
|
Not recorded | MAN alpha-D-mannopyranose × 21 MG MAGNESIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.8；277 K；100 mM NaCl, 5 % (w/v) PEG4000, 10 mM MgCl2, 100 mM Sodium Cacodylate trihydrate pH 5.8.
|
Resolution 6.15 Å R-free 0.271 |
| 6S0B Crystal Structure of Properdin in complex with the CTC domain of C3/C3b Deposited 2019-06-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric |
Chain C
1517–1663（147 aa）
|
Not recorded | MAN alpha-D-mannopyranose × 8 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5；291 K；Tacsimate, PEG 3350
|
Resolution 2.31 Å R-free 0.277 |
| 7AKK Structure of a complement factor-receptor complex Deposited 2020-10-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain A
749–1663（915 aa）
Chain B
23–667（645 aa）
Chain C
23–667（645 aa）
Chain E
749–1663（915 aa）
|
Not recorded | GOL GLYCEROL × 3 K POTASSIUM ION × 2 MG MAGNESIUM ION × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；295 K；100 mM Tris-HCl (pH 8.0), 8% (w/v) polyethylene glycol (PEG) 8000
|
Resolution 3.40 Å R-free 0.229 |
| 7BAG C3b in complex with CP40 Deposited 2020-12-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
23–667（645 aa）
Chain B
749–1663（915 aa）
|
Not recorded | CA CALCIUM ION × 1 PEG DI(HYDROXYETHYL)ETHER × 5 ACM ACETAMIDE × 1 NH2 AMINO GROUP × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；291 K；6.66% w/v polyethylene glycol 8000, 66.6 mM sodium chloride and 33.3 mM disodium phosphate/citric acid pH 4.0
|
Resolution 2.00 Å R-free 0.225 |
| 7NOZ Structure of the nanobody stablized properdin bound alternative pathway proconvertase C3b:FB:FP Deposited 2021-02-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain A
23–667（645 aa）
Chain B
752–1663（912 aa）
|
Not recorded | MAN alpha-D-mannopyranose × 6 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；0.05 M Na-acetate pH 5.3, 0.1 M Mg-formate,7% PEG5000 MME
|
Resolution 3.90 Å R-free 0.265 |
| 7PI6 Trypanosoma brucei ISG65 bound to human complement C3d Deposited 2021-08-19 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
996–1287（292 aa）
|
Not recorded | GOL GLYCEROL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；291 K；0.1 M MES pH 6, 0.2 M MgCl2, 20% PEG 6000 with sliver bullet HR2-996-55
|
Resolution 2.60 Å R-free 0.310 |
| 7PI6 Trypanosoma brucei ISG65 bound to human complement C3d Deposited 2021-08-19 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain D
996–1287（292 aa）
|
Not recorded | GOL GLYCEROL × 2 PG0 2-(2-METHOXYETHOXY)ETHANOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；291 K；0.1 M MES pH 6, 0.2 M MgCl2, 20% PEG 6000 with sliver bullet HR2-996-55
|
Resolution 2.60 Å R-free 0.310 |
| 7QIV Structure of human C3b in complex with the EWE nanobody Deposited 2021-12-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
23–667（645 aa）
Chain B
749–1663（915 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；292 K；112 mM Sodium Citrate pH 8, 28 mM Sodium Citrate pH 6.0, 12.25 % (w/v) PEG 2000 MME, 70 mM Imidazole pH 7, 60 mM Ammonium acetate, 70 mM TRIS pH 8.5, and 13.5 % (v/v) 2-Methyl-2,4-pentanediol
|
Resolution 2.80 Å R-free 0.275 |
| 7TV9 HUMAN COMPLEMENT COMPONENT C3B IN COMPLEX WITH APL-1030 Deposited 2022-02-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
23–667（645 aa）
Chain B
749–1663（915 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.8；293 K；100 mM HEPES, pH 7.8, 25% Poly(acrylic acid sodium salt) 5100
|
Resolution 3.40 Å R-free 0.328 |
| 7UE9 Structure of anti-C3d Fab(3d8b) in complex with C3d Deposited 2022-03-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain C
994–1303（310 aa）
|
Not recorded | GOL GLYCEROL × 10 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；15% PEG 10,000, 0.1 M bicine pH 7.6
|
Resolution 1.75 Å R-free 0.201 |
| 7ZGJ Trypanosoma brucei gambiense ISG65 in complex with human complement component C3 Deposited 2022-04-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
23–667（645 aa）
Chain B
672–1663（992 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.58 Å |
| 7ZGK Trypanosoma brucei gambiense ISG65 in complex with human complement component C3b Deposited 2022-04-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
23–667（645 aa）
Chain B
749–1663（915 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.59 Å |
| 8ENU Structure of the C3bB proconvertase in complex with lufaxin Deposited 2022-09-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain G
23–667（645 aa）
Chain H
749–1663（915 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.22 Å |
| 8EOK Structure of the C3bB proconvertase in complex with lufaxin and factor Xa Deposited 2022-10-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain G
23–667（645 aa）
Chain H
749–1663（915 aa）
|
Not recorded | MG MAGNESIUM ION × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.53 Å |
| 8HK2 C3aR-Gi-C3a protein complex Deposited 2022-11-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric |
Chain D
672–748（77 aa）
|
Not recorded | CLR CHOLESTEROL × 5 PLM PALMITIC ACID × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.2
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 8I9L Structure of C3a-C3aR-Go complex (Composite map) Deposited 2023-02-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric |
Chain D
672–748（77 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.18 Å |
| 8OQ3 Structure of methylamine treated human complement C3 Deposited 2023-04-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain A
23–1663（1641 aa）
Chain D
23–1663（1641 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 8OVB Human Complement C3b in complex with Trypanosoma brucei ISG65. Deposited 2023-04-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
23–664（642 aa）
Chain B
749–1663（915 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 8UH2 Complex of C3b with the inhibitor albicin Deposited 2023-10-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric |
Chain A
23–664（642 aa）
Chain B
749–1663（915 aa）
Chain G
23–664（642 aa）
Chain H
749–1663（915 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.59 Å |
| 8UIN Structure of the C3bBb-albicin complex Deposited 2023-10-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
23–664（642 aa）
Chain B
749–1663（915 aa）
Chain G
23–664（642 aa）
Chain H
749–1663（915 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；10 mM Hepes pH 7.4, 150 mM NaCl, 5 mM MgCl2
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.86 Å |
| 9N1Z Structure of C3d Bound to a Fragment of FHR-2 Deposited 2025-01-27 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
996–1287（292 aa）
|
Mutation：C1010A | SO4 SULFATE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；0.075 M HEPES [PH 7.5]
1.2 M Ammonium Sulfate
|
Resolution 2.31 Å R-free 0.227 |
| 9N1Z Structure of C3d Bound to a Fragment of FHR-2 Deposited 2025-01-27 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
996–1287（292 aa）
|
Mutation：C1010A | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；0.075 M HEPES [PH 7.5]
1.2 M Ammonium Sulfate
|
Resolution 2.31 Å R-free 0.227 |
| 9N20 Structure of C3d Bound to a Fragment of FHR-2 and S. aureus Efb-C Deposited 2025-01-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
996–1287（292 aa）
Fragment：residues 996-1287
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；0.1 M imidazole [pH 6.7]
12% (w/v) peg-20k
|
Resolution 3.30 Å R-free 0.214 |
| 9QK2 Structure of the Complement classical and lectin pathway C3 convertase in complex with substrate C3 Deposited 2025-03-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric |
Chain F
1–1663（1663 aa）
Chain G
1–1663（1663 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 MG MAGNESIUM ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 9RBO A cryo-EM structure of native C3 protein in a compact conformation. Deposited 2025-05-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
32–227（196 aa）
Chain B
350–665（316 aa）
Chain C
686–734（49 aa）
Chain D
762–1663（902 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY mmCIF provides none of the parsed conditions | Resolution 3.00 Å |
| 9T3Y Cryo-EM structure of alphaM/beta2:C3d-anti-CR3-Nb headpiece complex (HPO2 3D class reconstruction) Deposited 2025-10-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric |
Chain C
995–1289（295 aa）
|
Not recorded | CA CALCIUM ION × 5 MN MANGANESE (II) ION × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；0.02 % w/v CHAPS added to sample just before vitrification
|
Resolution 3.44 Å |
| 9U61 CP/MBL pathways C3 convertase C4b2a and C3 complex Deposited 2025-03-22 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
1–1663（1663 aa）
Chain D
1–1663（1663 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 NI NICKEL (II) ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9U62 AP pathways C3 convertase C3bBbP and C3 complex Deposited 2025-03-22 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric |
Chain C
1–1663（1663 aa）
Chain D
1–1663（1663 aa）
Chain E
1–1663（1663 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 5 NI NICKEL (II) ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
74 other PDB entries and 117 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | CO3_HUMAN |
| Isoform | — |
| PDB entities | 1 |
| Chains and sequence ranges | Author chain A； PDBConstruct 4–298； UniProt 994–1288 |
