← Back to search
5c3i

Crystal structure of the quaternary complex of histone H3-H4 heterodimer with chaperone ASF1 and the replicative helicase subunit MCM2

Method: X-RAY DIFFRACTION
▼

1. Protein Identity and Related Structures Protein Identity & Related Structures

Histone chaperone ASF1A

Homo sapiens

UniProt Q9Y294

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 4 Histone H3.1 × 1 （P68431） Histone H4 × 1 （P62805） DNA replication licensing factor MCM2,MCM2 × 1 （P49736） Consistent with protein count
2 Protein heterocomplex Heteromer Protein 4 Histone H3.1 × 1 （P68431） Histone H4 × 1 （P62805） DNA replication licensing factor MCM2,MCM2 × 1 （P49736） Consistent with protein count
3 Protein heterocomplex Heteromer Protein 4 Histone H3.1 × 1 （P68431） Histone H4 × 1 （P62805） DNA replication licensing factor MCM2,MCM2 × 1 （P49736） Consistent with protein count
4 Protein heterocomplex Heteromer Protein 4 Histone H3.1 × 1 （P68431） Histone H4 × 1 （P62805） DNA replication licensing factor MCM2,MCM2 × 1 （P49736） Consistent with protein count
5 Protein heterocomplex Heteromer Protein 4 Histone H3.1 × 1 （P68431） Histone H4 × 1 （P62805） DNA replication licensing factor MCM2,MCM2 × 1 （P49736） Consistent with protein count
6 Protein heterocomplex Heteromer Protein 4 Histone H3.1 × 1 （P68431） Histone H4 × 1 （P62805） DNA replication licensing factor MCM2,MCM2 × 1 （P49736） Consistent with protein count

Other States of the Same Protein in the Database

Monomer
1tey 7lny 7lny 7lny 7lny 7lny 7lny 7lny

8 assemblies

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
2i32 2i32 3aad 5c3i 5c3i 5c3i 5c3i 5c3i 5c3i 6f0f 6f0g 6f0g

37 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name ASF1A_HUMAN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 14–188； UniProt 1–175 Author chain E； PDBConstruct 14–188； UniProt 1–175 Author chain I； PDBConstruct 14–188； UniProt 1–175 Author chain M； PDBConstruct 14–188； UniProt 1–175 Author chain Q； PDBConstruct 14–188； UniProt 1–175 Author chain U； PDBConstruct 14–188； UniProt 1–175

Histone H3.1

Homo sapiens

UniProt P68431

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 4 Histone chaperone ASF1A × 1 （Q9Y294） Histone H4 × 1 （P62805） DNA replication licensing factor MCM2,MCM2 × 1 （P49736） Consistent with protein count
2 Protein heterocomplex Heteromer Protein 4 Histone chaperone ASF1A × 1 （Q9Y294） Histone H4 × 1 （P62805） DNA replication licensing factor MCM2,MCM2 × 1 （P49736） Consistent with protein count
3 Protein heterocomplex Heteromer Protein 4 Histone chaperone ASF1A × 1 （Q9Y294） Histone H4 × 1 （P62805） DNA replication licensing factor MCM2,MCM2 × 1 （P49736） Consistent with protein count
4 Protein heterocomplex Heteromer Protein 4 Histone chaperone ASF1A × 1 （Q9Y294） Histone H4 × 1 （P62805） DNA replication licensing factor MCM2,MCM2 × 1 （P49736） Consistent with protein count
5 Protein heterocomplex Heteromer Protein 4 Histone chaperone ASF1A × 1 （Q9Y294） Histone H4 × 1 （P62805） DNA replication licensing factor MCM2,MCM2 × 1 （P49736） Consistent with protein count
6 Protein heterocomplex Heteromer Protein 4 Histone chaperone ASF1A × 1 （Q9Y294） Histone H4 × 1 （P62805） DNA replication licensing factor MCM2,MCM2 × 1 （P49736） Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
2b2t 2b2u 2b2v 2b2w 2cv5 2l75 2ri7 2uxn 3a1b 3afa 3ayw 3aze

476 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name H31_HUMAN
Isoform —
PDB entities 2
Chains and sequence ranges Author chain B； PDBConstruct 1–136； UniProt 1–136 Author chain F； PDBConstruct 1–136； UniProt 1–136 Author chain J； PDBConstruct 1–136； UniProt 1–136 Author chain N； PDBConstruct 1–136； UniProt 1–136 Author chain R； PDBConstruct 1–136； UniProt 1–136 Author chain V； PDBConstruct 1–136； UniProt 1–136

Histone H4

Homo sapiens

UniProt P62805

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 4 Histone chaperone ASF1A × 1 （Q9Y294） Histone H3.1 × 1 （P68431） DNA replication licensing factor MCM2,MCM2 × 1 （P49736） Consistent with protein count
2 Protein heterocomplex Heteromer Protein 4 Histone chaperone ASF1A × 1 （Q9Y294） Histone H3.1 × 1 （P68431） DNA replication licensing factor MCM2,MCM2 × 1 （P49736） Consistent with protein count
3 Protein heterocomplex Heteromer Protein 4 Histone chaperone ASF1A × 1 （Q9Y294） Histone H3.1 × 1 （P68431） DNA replication licensing factor MCM2,MCM2 × 1 （P49736） Consistent with protein count
4 Protein heterocomplex Heteromer Protein 4 Histone chaperone ASF1A × 1 （Q9Y294） Histone H3.1 × 1 （P68431） DNA replication licensing factor MCM2,MCM2 × 1 （P49736） Consistent with protein count
5 Protein heterocomplex Heteromer Protein 4 Histone chaperone ASF1A × 1 （Q9Y294） Histone H3.1 × 1 （P68431） DNA replication licensing factor MCM2,MCM2 × 1 （P49736） Consistent with protein count
6 Protein heterocomplex Heteromer Protein 4 Histone chaperone ASF1A × 1 （Q9Y294） Histone H3.1 × 1 （P68431） DNA replication licensing factor MCM2,MCM2 × 1 （P49736） Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
10xz 10ya 10yb 10yc 10yd 10ye 10yf 10yg 10yh 10yi 2bqz 2bqz

634 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name H4_HUMAN
Isoform —
PDB entities 3
Chains and sequence ranges Author chain C； PDBConstruct 1–103； UniProt 1–103 Author chain G； PDBConstruct 1–103； UniProt 1–103 Author chain K； PDBConstruct 1–103； UniProt 1–103 Author chain O； PDBConstruct 1–103； UniProt 1–103 Author chain S； PDBConstruct 1–103； UniProt 1–103 Author chain W； PDBConstruct 1–103； UniProt 1–103

DNA replication licensing factor MCM2,MCM2

Homo sapiens

UniProt P49736

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 4 Histone chaperone ASF1A × 1 （Q9Y294） Histone H3.1 × 1 （P68431） Histone H4 × 1 （P62805） Consistent with protein count
2 Protein heterocomplex Heteromer Protein 4 Histone chaperone ASF1A × 1 （Q9Y294） Histone H3.1 × 1 （P68431） Histone H4 × 1 （P62805） Consistent with protein count
3 Protein heterocomplex Heteromer Protein 4 Histone chaperone ASF1A × 1 （Q9Y294） Histone H3.1 × 1 （P68431） Histone H4 × 1 （P62805） Consistent with protein count
4 Protein heterocomplex Heteromer Protein 4 Histone chaperone ASF1A × 1 （Q9Y294） Histone H3.1 × 1 （P68431） Histone H4 × 1 （P62805） Consistent with protein count
5 Protein heterocomplex Heteromer Protein 4 Histone chaperone ASF1A × 1 （Q9Y294） Histone H3.1 × 1 （P68431） Histone H4 × 1 （P62805） Consistent with protein count
6 Protein heterocomplex Heteromer Protein 4 Histone chaperone ASF1A × 1 （Q9Y294） Histone H3.1 × 1 （P68431） Histone H4 × 1 （P62805） Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
22vt 4uuz 5bnv 5bnx 5bo0 5c3i 5c3i 5c3i 5c3i 5c3i 5c3i 5ja4

47 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name MCM2_HUMAN
Isoform —
PDB entities 4
Chains and sequence ranges Author chain D； PDBConstruct 2–63； UniProt 63–124 Author chain H； PDBConstruct 2–63； UniProt 63–124 Author chain L； PDBConstruct 2–63； UniProt 63–124 Author chain P； PDBConstruct 2–63； UniProt 63–124 Author chain T； PDBConstruct 2–63； UniProt 63–124 Author chain X； PDBConstruct 2–63； UniProt 63–124

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

▼

2. Structure Basics 2. Structure Basics

Entry ID entry_id5c3i
Deposition date deposition_date2015-06-17
Structure title titleCrystal structure of the quaternary complex of histone H3-H4 heterodimer with chaperone ASF1 and the replicative helicase subunit MCM2
Keywords keywordsreplication, histone, chaperone, ASF1, MCM protein; REPLICATION
Experimental Method methodX-RAY DIFFRACTION
▼

3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

5c3i__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

5c3i__assembly_1__model_1 | I(q)

10-2 10-1 105 106 107 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

5c3i__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）24.07 Å
Rg (electron density)22.90 Å
Total Rg23.81 Å
Atom count2972
Residues369
Excluded volume52762 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 5c3i__assembly_1__model_1 tetrameric （4） Success 4.1.3-1-20251215 (887e7ef) View Download
2 1 5c3i__assembly_2__model_1 tetrameric （4） Success 4.1.3-1-20251215 (887e7ef) View Download
3 1 5c3i__assembly_3__model_1 tetrameric （4） Success 4.1.3-1-20251215 (887e7ef) View Download
4 1 5c3i__assembly_4__model_1 tetrameric （4） Success 4.1.3-1-20251215 (887e7ef) View Download
5 1 5c3i__assembly_5__model_1 tetrameric （4） Success 4.1.3-1-20251215 (887e7ef) View Download
6 1 5c3i__assembly_6__model_1 tetrameric （4） Success 4.1.3-1-20251215 (887e7ef) View Download
▶

4. Crystallography and Experiment 4. Crystallography & Experiment

▶

5. Entities and Polymers Entities & Polymers (4)

▼

6. Fold Classification (SCOP + CATH) 18 domains

CATH v4.4 (18 domains)

Domain ID domain_id5c3iA00
Class class2 — Mainly Beta
Architecture architecture60 — Sandwich
Topology topology40 — Immunoglobulin-like
Homologous superfamily homologous superfamily1490 — Histone chaperone ASF1-like
Domain ID domain_id5c3iB00
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology20 — Histone, subunit A
Homologous superfamily homologous superfamily10 — Histone, subunit A
Domain ID domain_id5c3iC00
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology20 — Histone, subunit A
Homologous superfamily homologous superfamily10 — Histone, subunit A
Domain ID domain_id5c3iE00
Class class2 — Mainly Beta
Architecture architecture60 — Sandwich
Topology topology40 — Immunoglobulin-like
Homologous superfamily homologous superfamily1490 — Histone chaperone ASF1-like
Domain ID domain_id5c3iF00
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology20 — Histone, subunit A
Homologous superfamily homologous superfamily10 — Histone, subunit A
Domain ID domain_id5c3iG00
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology20 — Histone, subunit A
Homologous superfamily homologous superfamily10 — Histone, subunit A
Domain ID domain_id5c3iI00
Class class2 — Mainly Beta
Architecture architecture60 — Sandwich
Topology topology40 — Immunoglobulin-like
Homologous superfamily homologous superfamily1490 — Histone chaperone ASF1-like
Domain ID domain_id5c3iJ00
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology20 — Histone, subunit A
Homologous superfamily homologous superfamily10 — Histone, subunit A
Domain ID domain_id5c3iK00
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology20 — Histone, subunit A
Homologous superfamily homologous superfamily10 — Histone, subunit A
Domain ID domain_id5c3iM00
Class class2 — Mainly Beta
Architecture architecture60 — Sandwich
Topology topology40 — Immunoglobulin-like
Homologous superfamily homologous superfamily1490 — Histone chaperone ASF1-like
Domain ID domain_id5c3iN00
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology20 — Histone, subunit A
Homologous superfamily homologous superfamily10 — Histone, subunit A
Domain ID domain_id5c3iO00
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology20 — Histone, subunit A
Homologous superfamily homologous superfamily10 — Histone, subunit A
Domain ID domain_id5c3iQ00
Class class2 — Mainly Beta
Architecture architecture60 — Sandwich
Topology topology40 — Immunoglobulin-like
Homologous superfamily homologous superfamily1490 — Histone chaperone ASF1-like
Domain ID domain_id5c3iR00
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology20 — Histone, subunit A
Homologous superfamily homologous superfamily10 — Histone, subunit A
Domain ID domain_id5c3iS00
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology20 — Histone, subunit A
Homologous superfamily homologous superfamily10 — Histone, subunit A
Domain ID domain_id5c3iU00
Class class2 — Mainly Beta
Architecture architecture60 — Sandwich
Topology topology40 — Immunoglobulin-like
Homologous superfamily homologous superfamily1490 — Histone chaperone ASF1-like
Domain ID domain_id5c3iV00
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology20 — Histone, subunit A
Homologous superfamily homologous superfamily10 — Histone, subunit A
Domain ID domain_id5c3iW00
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology20 — Histone, subunit A
Homologous superfamily homologous superfamily10 — Histone, subunit A
▶

7. Citations (1)