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5kaf

RT XFEL structure of Photosystem II in the dark state at 3.0 A resolution

Method: X-RAY DIFFRACTION
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Photosystem II protein D1 1

OrganismNot specified

UniProt P0A444

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 40 PDB declaration: 40-meric（40） Consistent with protein copy count Chain A； UniProt 1–344 Chain a； UniProt 1–344 Fragment：UNP residues 1-344 Photosystem II CP47 reaction center protein × 2 （Q8DIQ1） Photosystem II CP43 reaction center protein × 2 （Q8DIF8） Photosystem II D2 protein × 2 （Q8CM25） Cytochrome b559 subunit alpha × 2 （Q8DIP0） Cytochrome b559 subunit beta × 2 （Q8DIN9） Photosystem II reaction center protein H × 2 （Q8DJ43） Photosystem II reaction center protein I × 2 （Q8DJZ6） Photosystem II reaction center protein J × 2 （P59087） Photosystem II reaction center protein K × 2 （Q9F1K9） Photosystem II reaction center protein L × 2 （Q8DIN8） Photosystem II reaction center protein M × 2 （Q8DHA7） Photosystem II manganese-stabilizing polypeptide × 2 （P0A431） Photosystem II reaction center protein T × 2 （Q8DIQ0） Photosystem II 12 kDa extrinsic protein × 2 （Q9F1L5） Cytochrome c-550 × 2 （P0A386） Photosystem II reaction center protein Ycf12 × 2 （Q8DJI1） Photosystem II reaction center X protein × 2 （Q9F1R6） Photosystem II reaction center protein Z × 2 （Q8DHJ2） Photosystem II protein Y × 2 （Q8DKM3） OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 UNL UNKNOWN LIGAND × 17 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION X-ray crystallization conditions：BATCH MODE；pH 7.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol. Resolution 3.00 Å R-free 0.303

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5KAF Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
11YO Single-conformation model re-refinement of 2F/S3-rich PSII intermediate structure at 2.09 Angstrom resolution Deposited 2026-03-18 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain A 1–344（344 aa）
Chain a 1–344（344 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 38 OEX CA-MN4-O5 CLUSTER × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 PHO PHEOPHYTIN A × 4 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.243
1S5L Architecture of the photosynthetic oxygen evolving center Deposited 2004-01-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain A 1–344（344 aa）
Not recorded FE FE (III) ION × 1 BCT BICARBONATE ION × 2 OEC OXYGEN EVOLVING SYSTEM × 1 CLA CHLOROPHYLL A × 36 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMT DODECYL-BETA-D-MALTOSIDE × 1 BCR BETA-CAROTENE × 7 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pseudo-batch hanging drop；pH 7.5；291 K；100mM HEPES, 100mM (NH4)2SO4, PEG 4000, C12E8, trimethyl lead acetate, pH 7.5, pseudo-batch hanging drop, temperature 291K
Resolution 3.50 Å R-free 0.342
1S5L Architecture of the photosynthetic oxygen evolving center Deposited 2004-01-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain a 1–344（344 aa）
Not recorded FE FE (III) ION × 1 BCT BICARBONATE ION × 2 OEC OXYGEN EVOLVING SYSTEM × 1 CLA CHLOROPHYLL A × 36 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMT DODECYL-BETA-D-MALTOSIDE × 1 BCR BETA-CAROTENE × 7 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pseudo-batch hanging drop；pH 7.5；291 K；100mM HEPES, 100mM (NH4)2SO4, PEG 4000, C12E8, trimethyl lead acetate, pH 7.5, pseudo-batch hanging drop, temperature 291K
Resolution 3.50 Å R-free 0.342
1W5C Photosystem II from Thermosynechococcus elongatus Deposited 2004-08-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric
Chain A 1–360（360 aa）
Not recorded CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 MN MANGANESE (II) ION × 4 FE2 FE (II) ION × 1 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 BCR BETA-CAROTENE × 1 HEC HEME C × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 3.20 Å
1W5C Photosystem II from Thermosynechococcus elongatus Deposited 2004-08-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric
Chain G 1–360（360 aa）
Not recorded CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 MN MANGANESE (II) ION × 4 FE2 FE (II) ION × 1 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 BCR BETA-CAROTENE × 1 HEC HEME C × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 3.20 Å
3KZI Crystal Structure of Monomeric Form of Cyanobacterial Photosystem II Deposited 2009-12-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain A 1–344（344 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 11 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 LMT DODECYL-BETA-D-MALTOSIDE × 7 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 BCT BICARBONATE ION × 1 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；291 K；PEG 400, PIPES, MES, CaCl2, pH 7, batch, temperature 291.0K
Resolution 3.60 Å R-free 0.308
4FBY fs X-ray diffraction of Photosystem II Deposited 2012-05-23 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain A 1–344（344 aa）
Chain G 1–344（344 aa）
Not recorded CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 OEC OXYGEN EVOLVING SYSTEM × 2 FE2 FE (II) ION × 2 BCR BETA-CAROTENE × 24 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 CL CHLORIDE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；298 K；4% PEG 2000, 5 mM CaCl2, 100 mM PIPES, pH 7.0, batch, temperature 298K
Resolution 6.56 Å R-free 0.385
4IXQ RT fs X-ray diffraction of Photosystem II, dark state Deposited 2013-01-27 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain A 1–360（360 aa）
Chain a 1–360（360 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 LMT DODECYL-BETA-D-MALTOSIDE × 14 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.70 Å R-free 0.314
4IXR RT fs X-ray diffraction of Photosystem II, first illuminated state Deposited 2013-01-27 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain A 1–360（360 aa）
Chain a 1–360（360 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 LMT DODECYL-BETA-D-MALTOSIDE × 14 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.90 Å R-free 0.313
4PBU Serial Time-resolved crystallography of Photosystem II using a femtosecond X-ray laser The S1 state Deposited 2014-04-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain A 11–344（334 aa）
Chain a 11–344（334 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 CA CALCIUM ION × 7 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 5.00 Å R-free 0.262
4PJ0 Structure of T.elongatus Photosystem II, rows of dimers crystal packing Deposited 2014-05-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain A 1–344（344 aa）
Chain a 1–344（344 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE FE (III) ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SO4 SULFATE ION × 10 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 29 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；pH 7.5；293 K；Batch crystallisation: 2 mM Chl a equivalent of PSIIcc mixed 1:1 with: 0.1 M TRIS (pH 7.5), 0.1 M (NH4)2SO4, 15 - 18 % PEG 5000 MME, post-crystallisation dehydration
Resolution 2.44 Å R-free 0.256
4RVY Serial Time resolved crystallography of Photosystem II using a femtosecond X-ray laser. The S state after two flashes (S3) Deposited 2014-11-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain A 11–344（334 aa）
Chain a 11–344（334 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 CA CALCIUM ION × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions Free interface diffusion；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000 , Free interface diffusion, temperature 283K, temperature 283.0K
Resolution 5.50 Å R-free 0.291
4TNH RT XFEL structure of Photosystem II in the dark state at 4.9 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain A 1–344（344 aa） Fragment：UNP residues 1-344
Chain a 1–344（344 aa） Fragment：UNP residues 1-344
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.90 Å R-free 0.292
4TNI RT XFEL structure of Photosystem II 500 ms after the third illumination at 4.6 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain A 1–344（344 aa） Fragment：UNP residues 1-344
Chain a 1–344（344 aa） Fragment：UNP residues 1-344
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 2 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.60 Å R-free 0.284
4TNJ RT XFEL structure of Photosystem II 500 ms after the 2nd illumination (2F) at 4.5 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain A 1–344（344 aa） Fragment：UNP residues 1-344
Chain a 1–344（344 aa） Fragment：UNP residues 1-344
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.50 Å R-free 0.284
4TNK RT XFEL structure of Photosystem II 250 microsec after the third illumination at 5.2 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain A 1–344（344 aa） Fragment：UNP residues 1-344
Chain a 1–344（344 aa） Fragment：UNP residues 1-344
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.20 Å R-free 0.289
4V62 Crystal Structure of cyanobacterial Photosystem II Deposited 2008-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain AA 1–344（344 aa） Fragment：UNP residues 1-344
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 3 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 13 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 7 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 CL CHLORIDE ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 7 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG2000, PIPES, magnesium chloride, pH7.0, batch, temperature 298K
Resolution 2.90 Å R-free 0.292
4V62 Crystal Structure of cyanobacterial Photosystem II Deposited 2008-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain BA 1–344（344 aa） Fragment：UNP residues 1-344
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 3 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 11 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 7 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 CL CHLORIDE ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 7 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG2000, PIPES, magnesium chloride, pH7.0, batch, temperature 298K
Resolution 2.90 Å R-free 0.292
4V82 Crystal structure of cyanobacterial Photosystem II in complex with terbutryn Deposited 2010-11-30 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain AA 1–344（344 aa）
Chain BA 1–344（344 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 70 MST 2-T-BUTYLAMINO-4-ETHYLAMINO-6-METHYLTHIO-S-TRIAZINE × 2 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 LMT DODECYL-BETA-D-MALTOSIDE × 16 DMS DIMETHYL SULFOXIDE × 8 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；Pipes, CaCl2, DMSO, PEG 2000, pH 7.0, batch, temperature 298K
Resolution 3.20 Å R-free 0.299
5E79 Macromolecular diffractive imaging using imperfect crystals Deposited 2015-10-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain A 11–344（334 aa）
Chain a 11–344（334 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 CA CALCIUM ION × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；283.18 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 3.50 Å R-free 0.331
5E7C Macromolecular diffractive imaging using imperfect crystals - Bragg data Deposited 2015-10-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain A 11–344（334 aa）
Chain a 11–344（334 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 FE2 FE (II) ION × 2 CA CALCIUM ION × 6 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 4.50 Å R-free 0.275
5H2F Crystal structure of the PsbM-deletion mutant of photosystem II Deposited 2016-10-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain A 11–344（334 aa） Fragment：UNP RESIDUES 11-344
Not recorded OEX CA-MN4-O5 CLUSTER × 1 FE2 FE (II) ION × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 3 LMT DODECYL-BETA-D-MALTOSIDE × 6 UNL UNKNOWN LIGAND × 13 DMS DIMETHYL SULFOXIDE × 67 BCT BICARBONATE ION × 1 CA CALCIUM ION × 2 HTG heptyl 1-thio-beta-D-glucopyranoside × 9 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6.1；285 K；PEG1450, sodium chloride, calcium chloride, magnesium sulfate
Resolution 2.20 Å R-free 0.226
5H2F Crystal structure of the PsbM-deletion mutant of photosystem II Deposited 2016-10-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain a 11–344（334 aa） Fragment：UNP RESIDUES 11-344
Not recorded OEX CA-MN4-O5 CLUSTER × 1 FE2 FE (II) ION × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 5 LMT DODECYL-BETA-D-MALTOSIDE × 5 UNL UNKNOWN LIGAND × 14 DMS DIMETHYL SULFOXIDE × 66 BCT BICARBONATE ION × 1 CA CALCIUM ION × 3 HTG heptyl 1-thio-beta-D-glucopyranoside × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6.1；285 K；PEG1450, sodium chloride, calcium chloride, magnesium sulfate
Resolution 2.20 Å R-free 0.226
5KAI NH3-bound RT XFEL structure of Photosystem II 500 ms after the 2nd illumination (2F) at 2.8 A resolution Deposited 2016-06-01 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain A 1–344（344 aa） Fragment：UNP residues 1-344
Chain a 1–344（344 aa） Fragment：UNP residues 1-344
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 UNL UNKNOWN LIGAND × 23 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol
Resolution 2.80 Å R-free 0.300
5MX2 Photosystem II depleted of the Mn4CaO5 cluster at 2.55 A resolution Deposited 2017-01-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain A 1–344（344 aa）
Not recorded CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 11 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LFA EICOSANE × 19 FE FE (III) ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 4 BCT BICARBONATE ION × 1 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 7.5；291 K；equal volumes of buffer: 0.1 M Tris-HCl pH 7.5, 0.1 M (NH4)2SO4, 14-18 % PEG 5000 MME and protein: 2 mM chlorophyll equivalent of PSII in C12E8 detergent, post crystallisation treatment
Resolution 2.20 Å R-free 0.260
5MX2 Photosystem II depleted of the Mn4CaO5 cluster at 2.55 A resolution Deposited 2017-01-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain a 1–344（344 aa）
Not recorded CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 11 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LFA EICOSANE × 20 FE FE (III) ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 4 BCT BICARBONATE ION × 1 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 7.5；291 K；equal volumes of buffer: 0.1 M Tris-HCl pH 7.5, 0.1 M (NH4)2SO4, 14-18 % PEG 5000 MME and protein: 2 mM chlorophyll equivalent of PSII in C12E8 detergent, post crystallisation treatment
Resolution 2.20 Å R-free 0.260
5TIS Room temperature XFEL structure of the native, doubly-illuminated photosystem II complex Deposited 2016-10-03 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain A 1–344（344 aa） Fragment：UNP residues 1-344
Chain a 1–344（344 aa） Fragment：UNP residues 1-344
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 UNL UNKNOWN LIGAND × 33 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol
Resolution 2.25 Å R-free 0.231
5ZZN Crystal structure of photosystem II from an SQDG-deficient mutant of Thermosynechococcus elongatus Deposited 2018-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain A 11–344（334 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 5 UNL UNKNOWN LIGAND × 27 LMT DODECYL-BETA-D-MALTOSIDE × 9 DMS DIMETHYL SULFOXIDE × 48 CA CALCIUM ION × 3 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 HTG heptyl 1-thio-beta-D-glucopyranoside × 12 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 GOL GLYCEROL × 2 FE2 FE (II) ION × 1 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20MM NACL, 10MM CACL2, 40MM MGSO4, 20MM MES BUFFER, PH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPERATURE 285K
Resolution 2.10 Å R-free 0.197
5ZZN Crystal structure of photosystem II from an SQDG-deficient mutant of Thermosynechococcus elongatus Deposited 2018-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain a 11–344（334 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 1 CL CHLORIDE ION × 3 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 5 UNL UNKNOWN LIGAND × 25 LMT DODECYL-BETA-D-MALTOSIDE × 10 DMS DIMETHYL SULFOXIDE × 47 CA CALCIUM ION × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 HTG heptyl 1-thio-beta-D-glucopyranoside × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 GOL GLYCEROL × 3 FE2 FE (II) ION × 1 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20MM NACL, 10MM CACL2, 40MM MGSO4, 20MM MES BUFFER, PH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPERATURE 285K
Resolution 2.10 Å R-free 0.197
6DHE RT XFEL structure of the dark-stable state of Photosystem II (0F, S1-rich) at 2.05 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain A 11–344（334 aa）
Chain a 11–344（334 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 8CT (6'R,11cis,11'cis,13cis,15cis)-4',5'-didehydro-5',6'-dihydro-beta,beta-carotene × 12 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 BCR BETA-CAROTENE × 10 UNL UNKNOWN LIGAND × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.05 Å R-free 0.241
6DHF RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.08 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain A 11–344（334 aa）
Chain a 11–344（334 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 UNL UNKNOWN LIGAND × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.247
6DHG RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.5 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain A 11–344（334 aa）
Chain a 11–344（334 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.50 Å R-free 0.246
6DHH RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.2 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain A 11–344（334 aa）
Chain a 11–344（334 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.20 Å R-free 0.264
6DHO RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.07 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain A 11–344（334 aa）
Chain a 11–344（334 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.07 Å R-free 0.247
6DHP RT XFEL structure of the three-flash state of Photosystem II (3F, S0-rich) at 2.04 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain A 11–344（334 aa）
Chain a 11–344（334 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 UNL UNKNOWN LIGAND × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.04 Å R-free 0.248
6W1O RT XFEL structure of the dark-stable state of Photosystem II (0F, S1-rich) at 2.08 Angstrom resolution Deposited 2020-03-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain A 11–344（334 aa）
Chain a 11–344（334 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.240
6W1P RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.26 Angstrom resolution Deposited 2020-03-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain A 11–344（334 aa）
Chain a 11–344（334 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.26 Å R-free 0.241
6W1Q RT XFEL structure of Photosystem II 50 microseconds after the second illumination at 2.27 Angstrom resolution Deposited 2020-03-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain A 1–344（344 aa）
Chain a 1–344（344 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 BCR BETA-CAROTENE × 22 STE STEARIC ACID × 35 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 HEC HEME C × 4 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.27 Å R-free 0.249
6W1R RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.23 Angstrom resolution Deposited 2020-03-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain A 1–344（344 aa）
Chain a 1–344（344 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.23 Å R-free 0.235
6W1T RT XFEL structure of Photosystem II 250 microseconds after the second illumination at 2.01 Angstrom resolution Deposited 2020-03-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain A 1–344（344 aa）
Chain a 1–344（344 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.01 Å R-free 0.228
6W1U RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.09 Angstrom resolution Deposited 2020-03-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain A 1–344（344 aa）
Chain a 1–344（344 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
6W1V RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2020-03-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain A 1–344（344 aa）
Chain a 1–344（344 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 STE STEARIC ACID × 38 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 PHO PHEOPHYTIN A × 4 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.241
7NHO Structure of PSII-M Deposited 2021-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain A 1–360（360 aa）
Not recorded FE FE (III) ION × 1 MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 BCT BICARBONATE ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.66 Å
7NHP Structure of PSII-I (PSII with Psb27, Psb28, and Psb34) Deposited 2021-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain A 1–360（360 aa）
Not recorded MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 FE FE (III) ION × 1 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.72 Å
7NHQ Structure of PSII-I prime (PSII with Psb28, and Psb34) Deposited 2021-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 17 PDB declaration: heptadecameric
Chain A 1–360（360 aa）
Not recorded FE FE (III) ION × 1 MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.68 Å
7RF1 RT XFEL structure of Photosystem II averaged across all S-states at 1.89 Angstrom resolution Deposited 2021-07-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain A 1–344（344 aa）
Chain a 1–344（344 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 STE STEARIC ACID × 55 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 1.89 Å R-free 0.214
7RF2 RT XFEL structure of dark-stable state of Photosystem II (0F, S1 rich) at 2.08 Angstrom Deposited 2021-07-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain A 1–344（344 aa）
Chain a 1–344（344 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 33 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.238
7RF3 RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.26 Angstrom resolution Deposited 2021-07-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain A 1–344（344 aa）
Chain a 1–344（344 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.26 Å R-free 0.238
7RF4 RT XFEL structure of Photosystem II 50 microseconds after the second illumination at 2.27 Angstrom resolution Deposited 2021-07-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain A 1–344（344 aa）
Chain a 1–344（344 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 BCR BETA-CAROTENE × 22 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.27 Å R-free 0.244
7RF5 RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.23 Angstrom resolution Deposited 2021-07-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain A 1–344（344 aa）
Chain a 1–344（344 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.23 Å R-free 0.233
7RF6 RT XFEL structure of Photosystem II 250 microseconds after the second illumination at 2.01 Angstrom resolution Deposited 2021-07-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain A 1–344（344 aa）
Chain a 1–344（344 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.01 Å R-free 0.227
7RF7 RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.09 Angstrom resolution Deposited 2021-07-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain A 1–344（344 aa）
Chain a 1–344（344 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
7RF8 RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2021-07-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain A 1–344（344 aa）
Chain a 1–344（344 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
8EZ5 RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2022-10-31 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain A 1–344（344 aa）
Chain a 1–344（344 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.234
8F4C RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.00 Angstrom resolution Deposited 2022-11-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain A 1–344（344 aa）
Chain a 1–344（344 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.224
8F4D RT XFEL structure of Photosystem II 50 microseconds after the third illumination at 2.15 Angstrom resolution Deposited 2022-11-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain A 1–344（344 aa）
Chain a 1–344（344 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.15 Å R-free 0.243
8F4E RT XFEL structure of Photosystem II 250 microseconds after the third illumination at 2.09 Angstrom resolution Deposited 2022-11-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain A 1–344（344 aa）
Chain a 1–344（344 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.233
8F4F RT XFEL structure of Photosystem II 500 microseconds after the third illumination at 2.03 Angstrom resolution Deposited 2022-11-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain A 1–344（344 aa）
Chain a 1–344（344 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.03 Å R-free 0.248
8F4G RT XFEL structure of Photosystem II 730 microseconds after the third illumination at 2.03 Angstrom resolution Deposited 2022-11-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain A 1–344（344 aa）
Chain a 1–344（344 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.03 Å R-free 0.223
8F4H RT XFEL structure of Photosystem II 1200 microseconds after the third illumination at 2.10 Angstrom resolution Deposited 2022-11-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain A 1–344（344 aa）
Chain a 1–344（344 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.10 Å R-free 0.232
8F4I RT XFEL structure of Photosystem II 2000 microseconds after the third illumination at 2.00 Angstrom resolution Deposited 2022-11-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain A 1–344（344 aa）
Chain a 1–344（344 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.232
8F4J RT XFEL structure of Photosystem II 4000 microseconds after the third illumination at 2.00 Angstrom resolution Deposited 2022-11-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain A 1–344（344 aa）
Chain a 1–344（344 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.226
8F4K RT XFEL structure of the three-flash state of Photosystem II (3F, S0-rich) at 2.16 Angstrom resolution Deposited 2022-11-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain A 1–344（344 aa）
Chain a 1–344（344 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.16 Å R-free 0.241
9EVX cryoEM structure of Photosystem II averaged across S2-S3 states at 1.71 Angstrom resolution Deposited 2024-04-02 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain A 1–344（344 aa）
Chain a 1–344（344 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 STE STEARIC ACID × 42 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 PHO PHEOPHYTIN A × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；100mM MES pH6.5, 5mM CaCl2, 5% glycerol, 0.03% DDM
cryo-EM vitrification conditions Cryogen ETHANE；The sample-on-grid was illuminated with one and two flashes of light
Resolution 1.71 Å
9G6F Inactive PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain A 1–360（360 aa）
Chain a 1–360（360 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LFA EICOSANE × 33 PLM PALMITIC ACID × 24 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 8 LMT DODECYL-BETA-D-MALTOSIDE × 13 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å
9G6G Semi-active PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric
Chain A 1–360（360 aa）
Chain a 1–360（360 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CL CHLORIDE ION × 3 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 LFA EICOSANE × 30 OEX CA-MN4-O5 CLUSTER × 1 LMT DODECYL-BETA-D-MALTOSIDE × 17 BCT BICARBONATE ION × 2 PLM PALMITIC ACID × 29 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 GOL GLYCEROL × 1 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 3 RRX (3R)-beta,beta-caroten-3-ol × 2 CA CALCIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å
9G6H active PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain A 1–360（360 aa）
Chain a 1–360（360 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 PLM PALMITIC ACID × 35 LFA EICOSANE × 24 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 GOL GLYCEROL × 2 LMT DODECYL-BETA-D-MALTOSIDE × 17 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 RRX (3R)-beta,beta-caroten-3-ol × 2 CA CALCIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å

61 other PDB entries and 67 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PSBA1_THEEB
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–344； UniProt 1–344 Author chain a； PDBConstruct 1–344； UniProt 1–344

Photosystem II CP47 reaction center protein

OrganismNot specified

UniProt Q8DIQ1

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 40 PDB declaration: 40-meric（40） Consistent with protein copy count Chain B； UniProt 1–510 Chain b； UniProt 1–510 Not recorded Photosystem II protein D1 1 × 2 （P0A444） Photosystem II CP43 reaction center protein × 2 （Q8DIF8） Photosystem II D2 protein × 2 （Q8CM25） Cytochrome b559 subunit alpha × 2 （Q8DIP0） Cytochrome b559 subunit beta × 2 （Q8DIN9） Photosystem II reaction center protein H × 2 （Q8DJ43） Photosystem II reaction center protein I × 2 （Q8DJZ6） Photosystem II reaction center protein J × 2 （P59087） Photosystem II reaction center protein K × 2 （Q9F1K9） Photosystem II reaction center protein L × 2 （Q8DIN8） Photosystem II reaction center protein M × 2 （Q8DHA7） Photosystem II manganese-stabilizing polypeptide × 2 （P0A431） Photosystem II reaction center protein T × 2 （Q8DIQ0） Photosystem II 12 kDa extrinsic protein × 2 （Q9F1L5） Cytochrome c-550 × 2 （P0A386） Photosystem II reaction center protein Ycf12 × 2 （Q8DJI1） Photosystem II reaction center X protein × 2 （Q9F1R6） Photosystem II reaction center protein Z × 2 （Q8DHJ2） Photosystem II protein Y × 2 （Q8DKM3） OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 UNL UNKNOWN LIGAND × 17 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION X-ray crystallization conditions：BATCH MODE；pH 7.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol. Resolution 3.00 Å R-free 0.303

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5KAF Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
11YO Single-conformation model re-refinement of 2F/S3-rich PSII intermediate structure at 2.09 Angstrom resolution Deposited 2026-03-18 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain B 1–510（510 aa）
Chain b 1–510（510 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 38 OEX CA-MN4-O5 CLUSTER × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 PHO PHEOPHYTIN A × 4 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.243
1S5L Architecture of the photosynthetic oxygen evolving center Deposited 2004-01-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain B 1–510（510 aa）
Not recorded FE FE (III) ION × 1 BCT BICARBONATE ION × 2 OEC OXYGEN EVOLVING SYSTEM × 1 CLA CHLOROPHYLL A × 36 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMT DODECYL-BETA-D-MALTOSIDE × 1 BCR BETA-CAROTENE × 7 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pseudo-batch hanging drop；pH 7.5；291 K；100mM HEPES, 100mM (NH4)2SO4, PEG 4000, C12E8, trimethyl lead acetate, pH 7.5, pseudo-batch hanging drop, temperature 291K
Resolution 3.50 Å R-free 0.342
1S5L Architecture of the photosynthetic oxygen evolving center Deposited 2004-01-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain b 1–510（510 aa）
Not recorded FE FE (III) ION × 1 BCT BICARBONATE ION × 2 OEC OXYGEN EVOLVING SYSTEM × 1 CLA CHLOROPHYLL A × 36 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMT DODECYL-BETA-D-MALTOSIDE × 1 BCR BETA-CAROTENE × 7 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pseudo-batch hanging drop；pH 7.5；291 K；100mM HEPES, 100mM (NH4)2SO4, PEG 4000, C12E8, trimethyl lead acetate, pH 7.5, pseudo-batch hanging drop, temperature 291K
Resolution 3.50 Å R-free 0.342
1W5C Photosystem II from Thermosynechococcus elongatus Deposited 2004-08-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric
Chain B 1–510（510 aa）
Not recorded CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 MN MANGANESE (II) ION × 4 FE2 FE (II) ION × 1 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 BCR BETA-CAROTENE × 1 HEC HEME C × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 3.20 Å
1W5C Photosystem II from Thermosynechococcus elongatus Deposited 2004-08-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric
Chain H 1–510（510 aa）
Not recorded CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 MN MANGANESE (II) ION × 4 FE2 FE (II) ION × 1 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 BCR BETA-CAROTENE × 1 HEC HEME C × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 3.20 Å
3KZI Crystal Structure of Monomeric Form of Cyanobacterial Photosystem II Deposited 2009-12-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain B 1–510（510 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 11 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 LMT DODECYL-BETA-D-MALTOSIDE × 7 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 BCT BICARBONATE ION × 1 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；291 K；PEG 400, PIPES, MES, CaCl2, pH 7, batch, temperature 291.0K
Resolution 3.60 Å R-free 0.308
4FBY fs X-ray diffraction of Photosystem II Deposited 2012-05-23 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain B 1–510（510 aa）
Chain N 1–510（510 aa）
Not recorded CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 OEC OXYGEN EVOLVING SYSTEM × 2 FE2 FE (II) ION × 2 BCR BETA-CAROTENE × 24 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 CL CHLORIDE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；298 K；4% PEG 2000, 5 mM CaCl2, 100 mM PIPES, pH 7.0, batch, temperature 298K
Resolution 6.56 Å R-free 0.385
4IXQ RT fs X-ray diffraction of Photosystem II, dark state Deposited 2013-01-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain B 1–510（510 aa）
Chain b 1–510（510 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 LMT DODECYL-BETA-D-MALTOSIDE × 14 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.70 Å R-free 0.314
4IXR RT fs X-ray diffraction of Photosystem II, first illuminated state Deposited 2013-01-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain B 1–510（510 aa）
Chain b 1–510（510 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 LMT DODECYL-BETA-D-MALTOSIDE × 14 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.90 Å R-free 0.313
4PBU Serial Time-resolved crystallography of Photosystem II using a femtosecond X-ray laser The S1 state Deposited 2014-04-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain B 2–505（504 aa）
Chain b 2–505（504 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 CA CALCIUM ION × 7 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 5.00 Å R-free 0.262
4RVY Serial Time resolved crystallography of Photosystem II using a femtosecond X-ray laser. The S state after two flashes (S3) Deposited 2014-11-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain B 2–505（504 aa）
Chain b 2–505（504 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 CA CALCIUM ION × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions Free interface diffusion；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000 , Free interface diffusion, temperature 283K, temperature 283.0K
Resolution 5.50 Å R-free 0.291
4TNH RT XFEL structure of Photosystem II in the dark state at 4.9 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain B 1–510（510 aa）
Chain b 1–510（510 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.90 Å R-free 0.292
4TNI RT XFEL structure of Photosystem II 500 ms after the third illumination at 4.6 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain B 1–510（510 aa）
Chain b 1–510（510 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 2 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.60 Å R-free 0.284
4TNJ RT XFEL structure of Photosystem II 500 ms after the 2nd illumination (2F) at 4.5 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain B 1–510（510 aa）
Chain b 1–510（510 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.50 Å R-free 0.284
4TNK RT XFEL structure of Photosystem II 250 microsec after the third illumination at 5.2 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain B 1–510（510 aa）
Chain b 1–510（510 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.20 Å R-free 0.289
4V62 Crystal Structure of cyanobacterial Photosystem II Deposited 2008-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain AB 1–510（510 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 3 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 13 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 7 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 CL CHLORIDE ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 7 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG2000, PIPES, magnesium chloride, pH7.0, batch, temperature 298K
Resolution 2.90 Å R-free 0.292
4V62 Crystal Structure of cyanobacterial Photosystem II Deposited 2008-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain BB 1–510（510 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 3 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 11 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 7 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 CL CHLORIDE ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 7 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG2000, PIPES, magnesium chloride, pH7.0, batch, temperature 298K
Resolution 2.90 Å R-free 0.292
4V82 Crystal structure of cyanobacterial Photosystem II in complex with terbutryn Deposited 2010-11-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain AB 1–510（510 aa）
Chain BB 1–510（510 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 70 MST 2-T-BUTYLAMINO-4-ETHYLAMINO-6-METHYLTHIO-S-TRIAZINE × 2 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 LMT DODECYL-BETA-D-MALTOSIDE × 16 DMS DIMETHYL SULFOXIDE × 8 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；Pipes, CaCl2, DMSO, PEG 2000, pH 7.0, batch, temperature 298K
Resolution 3.20 Å R-free 0.299
5E79 Macromolecular diffractive imaging using imperfect crystals Deposited 2015-10-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain B 2–505（504 aa）
Chain b 2–505（504 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 CA CALCIUM ION × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；283.18 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 3.50 Å R-free 0.331
5E7C Macromolecular diffractive imaging using imperfect crystals - Bragg data Deposited 2015-10-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain B 2–505（504 aa）
Chain b 2–505（504 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 FE2 FE (II) ION × 2 CA CALCIUM ION × 6 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 4.50 Å R-free 0.275
5H2F Crystal structure of the PsbM-deletion mutant of photosystem II Deposited 2016-10-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain B 2–506（505 aa） Fragment：UNP RESIDUES 2-506
Not recorded OEX CA-MN4-O5 CLUSTER × 1 FE2 FE (II) ION × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 3 LMT DODECYL-BETA-D-MALTOSIDE × 6 UNL UNKNOWN LIGAND × 13 DMS DIMETHYL SULFOXIDE × 67 BCT BICARBONATE ION × 1 CA CALCIUM ION × 2 HTG heptyl 1-thio-beta-D-glucopyranoside × 9 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6.1；285 K；PEG1450, sodium chloride, calcium chloride, magnesium sulfate
Resolution 2.20 Å R-free 0.226
5H2F Crystal structure of the PsbM-deletion mutant of photosystem II Deposited 2016-10-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain b 2–506（505 aa） Fragment：UNP RESIDUES 2-506
Not recorded OEX CA-MN4-O5 CLUSTER × 1 FE2 FE (II) ION × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 5 LMT DODECYL-BETA-D-MALTOSIDE × 5 UNL UNKNOWN LIGAND × 14 DMS DIMETHYL SULFOXIDE × 66 BCT BICARBONATE ION × 1 CA CALCIUM ION × 3 HTG heptyl 1-thio-beta-D-glucopyranoside × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6.1；285 K；PEG1450, sodium chloride, calcium chloride, magnesium sulfate
Resolution 2.20 Å R-free 0.226
5KAI NH3-bound RT XFEL structure of Photosystem II 500 ms after the 2nd illumination (2F) at 2.8 A resolution Deposited 2016-06-01 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain B 1–510（510 aa）
Chain b 1–510（510 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 UNL UNKNOWN LIGAND × 23 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol
Resolution 2.80 Å R-free 0.300
5MX2 Photosystem II depleted of the Mn4CaO5 cluster at 2.55 A resolution Deposited 2017-01-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain B 1–510（510 aa）
Not recorded CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 11 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LFA EICOSANE × 19 FE FE (III) ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 4 BCT BICARBONATE ION × 1 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 7.5；291 K；equal volumes of buffer: 0.1 M Tris-HCl pH 7.5, 0.1 M (NH4)2SO4, 14-18 % PEG 5000 MME and protein: 2 mM chlorophyll equivalent of PSII in C12E8 detergent, post crystallisation treatment
Resolution 2.20 Å R-free 0.260
5MX2 Photosystem II depleted of the Mn4CaO5 cluster at 2.55 A resolution Deposited 2017-01-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain b 1–510（510 aa）
Not recorded CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 11 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LFA EICOSANE × 20 FE FE (III) ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 4 BCT BICARBONATE ION × 1 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 7.5；291 K；equal volumes of buffer: 0.1 M Tris-HCl pH 7.5, 0.1 M (NH4)2SO4, 14-18 % PEG 5000 MME and protein: 2 mM chlorophyll equivalent of PSII in C12E8 detergent, post crystallisation treatment
Resolution 2.20 Å R-free 0.260
5TIS Room temperature XFEL structure of the native, doubly-illuminated photosystem II complex Deposited 2016-10-03 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain B 1–510（510 aa）
Chain b 1–510（510 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 UNL UNKNOWN LIGAND × 33 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol
Resolution 2.25 Å R-free 0.231
5ZZN Crystal structure of photosystem II from an SQDG-deficient mutant of Thermosynechococcus elongatus Deposited 2018-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain B 2–506（505 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 5 UNL UNKNOWN LIGAND × 27 LMT DODECYL-BETA-D-MALTOSIDE × 9 DMS DIMETHYL SULFOXIDE × 48 CA CALCIUM ION × 3 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 HTG heptyl 1-thio-beta-D-glucopyranoside × 12 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 GOL GLYCEROL × 2 FE2 FE (II) ION × 1 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20MM NACL, 10MM CACL2, 40MM MGSO4, 20MM MES BUFFER, PH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPERATURE 285K
Resolution 2.10 Å R-free 0.197
5ZZN Crystal structure of photosystem II from an SQDG-deficient mutant of Thermosynechococcus elongatus Deposited 2018-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain b 2–506（505 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 1 CL CHLORIDE ION × 3 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 5 UNL UNKNOWN LIGAND × 25 LMT DODECYL-BETA-D-MALTOSIDE × 10 DMS DIMETHYL SULFOXIDE × 47 CA CALCIUM ION × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 HTG heptyl 1-thio-beta-D-glucopyranoside × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 GOL GLYCEROL × 3 FE2 FE (II) ION × 1 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20MM NACL, 10MM CACL2, 40MM MGSO4, 20MM MES BUFFER, PH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPERATURE 285K
Resolution 2.10 Å R-free 0.197
6DHE RT XFEL structure of the dark-stable state of Photosystem II (0F, S1-rich) at 2.05 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain B 2–506（505 aa）
Chain b 2–506（505 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 8CT (6'R,11cis,11'cis,13cis,15cis)-4',5'-didehydro-5',6'-dihydro-beta,beta-carotene × 12 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 BCR BETA-CAROTENE × 10 UNL UNKNOWN LIGAND × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.05 Å R-free 0.241
6DHF RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.08 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain B 2–506（505 aa）
Chain b 2–506（505 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 UNL UNKNOWN LIGAND × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.247
6DHG RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.5 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain B 2–506（505 aa）
Chain b 2–506（505 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.50 Å R-free 0.246
6DHH RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.2 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain B 2–506（505 aa）
Chain b 2–506（505 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.20 Å R-free 0.264
6DHO RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.07 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain B 2–506（505 aa）
Chain b 2–506（505 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.07 Å R-free 0.247
6DHP RT XFEL structure of the three-flash state of Photosystem II (3F, S0-rich) at 2.04 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain B 2–506（505 aa）
Chain b 2–506（505 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 UNL UNKNOWN LIGAND × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.04 Å R-free 0.248
6W1O RT XFEL structure of the dark-stable state of Photosystem II (0F, S1-rich) at 2.08 Angstrom resolution Deposited 2020-03-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain B 1–506（506 aa）
Chain b 1–506（506 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.240
6W1P RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.26 Angstrom resolution Deposited 2020-03-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain B 1–506（506 aa）
Chain b 1–506（506 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.26 Å R-free 0.241
6W1Q RT XFEL structure of Photosystem II 50 microseconds after the second illumination at 2.27 Angstrom resolution Deposited 2020-03-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain B 1–506（506 aa）
Chain b 1–506（506 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 BCR BETA-CAROTENE × 22 STE STEARIC ACID × 35 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 HEC HEME C × 4 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.27 Å R-free 0.249
6W1R RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.23 Angstrom resolution Deposited 2020-03-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain B 1–506（506 aa）
Chain b 1–506（506 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.23 Å R-free 0.235
6W1T RT XFEL structure of Photosystem II 250 microseconds after the second illumination at 2.01 Angstrom resolution Deposited 2020-03-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain B 1–506（506 aa）
Chain b 1–506（506 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.01 Å R-free 0.228
6W1U RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.09 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain B 1–510（510 aa）
Chain b 1–510（510 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
6W1V RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain B 1–510（510 aa）
Chain b 1–510（510 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 STE STEARIC ACID × 38 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 PHO PHEOPHYTIN A × 4 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.241
7NHO Structure of PSII-M Deposited 2021-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain B 1–510（510 aa）
Not recorded FE FE (III) ION × 1 MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 BCT BICARBONATE ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.66 Å
7NHP Structure of PSII-I (PSII with Psb27, Psb28, and Psb34) Deposited 2021-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain B 1–510（510 aa）
Not recorded MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 FE FE (III) ION × 1 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.72 Å
7NHQ Structure of PSII-I prime (PSII with Psb28, and Psb34) Deposited 2021-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 17 PDB declaration: heptadecameric
Chain B 1–510（510 aa）
Not recorded FE FE (III) ION × 1 MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.68 Å
7RF1 RT XFEL structure of Photosystem II averaged across all S-states at 1.89 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain B 1–510（510 aa）
Chain b 1–510（510 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 STE STEARIC ACID × 55 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 1.89 Å R-free 0.214
7RF2 RT XFEL structure of dark-stable state of Photosystem II (0F, S1 rich) at 2.08 Angstrom Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain B 1–510（510 aa）
Chain b 1–510（510 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 33 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.238
7RF3 RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.26 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain B 1–510（510 aa）
Chain b 1–510（510 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.26 Å R-free 0.238
7RF4 RT XFEL structure of Photosystem II 50 microseconds after the second illumination at 2.27 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain B 1–510（510 aa）
Chain b 1–510（510 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 BCR BETA-CAROTENE × 22 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.27 Å R-free 0.244
7RF5 RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.23 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain B 1–510（510 aa）
Chain b 1–510（510 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.23 Å R-free 0.233
7RF6 RT XFEL structure of Photosystem II 250 microseconds after the second illumination at 2.01 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain B 1–510（510 aa）
Chain b 1–510（510 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.01 Å R-free 0.227
7RF7 RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.09 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain B 1–510（510 aa）
Chain b 1–510（510 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
7RF8 RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain B 1–510（510 aa）
Chain b 1–510（510 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
7YQ2 Crystal structure of photosystem II expressing psbA2 gene only Deposited 2022-08-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 39 PDB declaration: 39-meric
Chain B 1–510（510 aa）
Chain b 1–510（510 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 UNL UNKNOWN LIGAND × 77 LMT DODECYL-BETA-D-MALTOSIDE × 19 GOL GLYCEROL × 10 BCT BICARBONATE ION × 3 DMS DIMETHYL SULFOXIDE × 116 CA CALCIUM ION × 7 HTG heptyl 1-thio-beta-D-glucopyranoside × 31 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 9 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 MG MAGNESIUM ION × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20mM NaCl, 10mM CaCl2, 40mM MgSO4, 20mM Mes BUFFER, pH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPARATURE 285K
Resolution 1.90 Å R-free 0.184
7YQ7 Crystal structure of photosystem II expressing psbA3 gene only Deposited 2022-08-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 39 PDB declaration: 39-meric
Chain B 1–510（510 aa）
Chain b 1–510（510 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 UNL UNKNOWN LIGAND × 82 GOL GLYCEROL × 11 LMT DODECYL-BETA-D-MALTOSIDE × 18 BCT BICARBONATE ION × 4 DMS DIMETHYL SULFOXIDE × 71 CA CALCIUM ION × 5 HTG heptyl 1-thio-beta-D-glucopyranoside × 18 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 MG MAGNESIUM ION × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20mM NaCl, 10mM CaCl2, 40mM MgSO4, 20mM MES BUFFER pH 6.0, MICRO-BATCH METHOD UNDER OIL, TMPERATURE 285K
Resolution 1.90 Å R-free 0.177
8EZ5 RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2022-10-31 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain B 1–510（510 aa）
Chain b 1–510（510 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.234
8F4C RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain B 1–510（510 aa）
Chain b 1–510（510 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.224
8F4D RT XFEL structure of Photosystem II 50 microseconds after the third illumination at 2.15 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain B 1–510（510 aa）
Chain b 1–510（510 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.15 Å R-free 0.243
8F4E RT XFEL structure of Photosystem II 250 microseconds after the third illumination at 2.09 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain B 1–510（510 aa）
Chain b 1–510（510 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.233
8F4F RT XFEL structure of Photosystem II 500 microseconds after the third illumination at 2.03 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain B 1–510（510 aa）
Chain b 1–510（510 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.03 Å R-free 0.248
8F4G RT XFEL structure of Photosystem II 730 microseconds after the third illumination at 2.03 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain B 1–510（510 aa）
Chain b 1–510（510 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.03 Å R-free 0.223
8F4H RT XFEL structure of Photosystem II 1200 microseconds after the third illumination at 2.10 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain B 1–510（510 aa）
Chain b 1–510（510 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.10 Å R-free 0.232
8F4I RT XFEL structure of Photosystem II 2000 microseconds after the third illumination at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain B 1–510（510 aa）
Chain b 1–510（510 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.232
8F4J RT XFEL structure of Photosystem II 4000 microseconds after the third illumination at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain B 1–510（510 aa）
Chain b 1–510（510 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.226
8F4K RT XFEL structure of the three-flash state of Photosystem II (3F, S0-rich) at 2.16 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain B 1–510（510 aa）
Chain b 1–510（510 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.16 Å R-free 0.241
9EVX cryoEM structure of Photosystem II averaged across S2-S3 states at 1.71 Angstrom resolution Deposited 2024-04-02 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain B 1–510（510 aa）
Chain b 1–510（510 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 STE STEARIC ACID × 42 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 PHO PHEOPHYTIN A × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；100mM MES pH6.5, 5mM CaCl2, 5% glycerol, 0.03% DDM
cryo-EM vitrification conditions Cryogen ETHANE；The sample-on-grid was illuminated with one and two flashes of light
Resolution 1.71 Å
9G6F Inactive PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain B 1–510（510 aa）
Chain b 1–510（510 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LFA EICOSANE × 33 PLM PALMITIC ACID × 24 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 8 LMT DODECYL-BETA-D-MALTOSIDE × 13 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å
9G6G Semi-active PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric
Chain B 1–510（510 aa）
Chain b 1–510（510 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 3 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 LFA EICOSANE × 30 OEX CA-MN4-O5 CLUSTER × 1 LMT DODECYL-BETA-D-MALTOSIDE × 17 BCT BICARBONATE ION × 2 PLM PALMITIC ACID × 29 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 GOL GLYCEROL × 1 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 3 RRX (3R)-beta,beta-caroten-3-ol × 2 CA CALCIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å
9G6H active PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain B 1–510（510 aa）
Chain b 1–510（510 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 PLM PALMITIC ACID × 35 LFA EICOSANE × 24 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 GOL GLYCEROL × 2 LMT DODECYL-BETA-D-MALTOSIDE × 17 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 RRX (3R)-beta,beta-caroten-3-ol × 2 CA CALCIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å

62 other PDB entries and 68 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PSBB_THEEB
Isoform —
PDB entities 2
Chains and sequence ranges Author chain B； PDBConstruct 1–510； UniProt 1–510 Author chain b； PDBConstruct 1–510； UniProt 1–510

Photosystem II CP43 reaction center protein

OrganismNot specified

UniProt Q8DIF8

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 40 PDB declaration: 40-meric（40） Consistent with protein copy count Chain C； UniProt 1–461 Chain c； UniProt 1–461 Not recorded Photosystem II protein D1 1 × 2 （P0A444） Photosystem II CP47 reaction center protein × 2 （Q8DIQ1） Photosystem II D2 protein × 2 （Q8CM25） Cytochrome b559 subunit alpha × 2 （Q8DIP0） Cytochrome b559 subunit beta × 2 （Q8DIN9） Photosystem II reaction center protein H × 2 （Q8DJ43） Photosystem II reaction center protein I × 2 （Q8DJZ6） Photosystem II reaction center protein J × 2 （P59087） Photosystem II reaction center protein K × 2 （Q9F1K9） Photosystem II reaction center protein L × 2 （Q8DIN8） Photosystem II reaction center protein M × 2 （Q8DHA7） Photosystem II manganese-stabilizing polypeptide × 2 （P0A431） Photosystem II reaction center protein T × 2 （Q8DIQ0） Photosystem II 12 kDa extrinsic protein × 2 （Q9F1L5） Cytochrome c-550 × 2 （P0A386） Photosystem II reaction center protein Ycf12 × 2 （Q8DJI1） Photosystem II reaction center X protein × 2 （Q9F1R6） Photosystem II reaction center protein Z × 2 （Q8DHJ2） Photosystem II protein Y × 2 （Q8DKM3） OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 UNL UNKNOWN LIGAND × 17 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION X-ray crystallization conditions：BATCH MODE；pH 7.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol. Resolution 3.00 Å R-free 0.303

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5KAF Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
11YO Single-conformation model re-refinement of 2F/S3-rich PSII intermediate structure at 2.09 Angstrom resolution Deposited 2026-03-18 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain C 1–461（461 aa）
Chain c 1–461（461 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 38 OEX CA-MN4-O5 CLUSTER × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 PHO PHEOPHYTIN A × 4 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.243
1S5L Architecture of the photosynthetic oxygen evolving center Deposited 2004-01-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain C 2–461（460 aa）
Not recorded FE FE (III) ION × 1 BCT BICARBONATE ION × 2 OEC OXYGEN EVOLVING SYSTEM × 1 CLA CHLOROPHYLL A × 36 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMT DODECYL-BETA-D-MALTOSIDE × 1 BCR BETA-CAROTENE × 7 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pseudo-batch hanging drop；pH 7.5；291 K；100mM HEPES, 100mM (NH4)2SO4, PEG 4000, C12E8, trimethyl lead acetate, pH 7.5, pseudo-batch hanging drop, temperature 291K
Resolution 3.50 Å R-free 0.342
1S5L Architecture of the photosynthetic oxygen evolving center Deposited 2004-01-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain c 2–461（460 aa）
Not recorded FE FE (III) ION × 1 BCT BICARBONATE ION × 2 OEC OXYGEN EVOLVING SYSTEM × 1 CLA CHLOROPHYLL A × 36 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMT DODECYL-BETA-D-MALTOSIDE × 1 BCR BETA-CAROTENE × 7 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pseudo-batch hanging drop；pH 7.5；291 K；100mM HEPES, 100mM (NH4)2SO4, PEG 4000, C12E8, trimethyl lead acetate, pH 7.5, pseudo-batch hanging drop, temperature 291K
Resolution 3.50 Å R-free 0.342
1W5C Photosystem II from Thermosynechococcus elongatus Deposited 2004-08-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric
Chain C 1–473（473 aa）
Not recorded CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 MN MANGANESE (II) ION × 4 FE2 FE (II) ION × 1 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 BCR BETA-CAROTENE × 1 HEC HEME C × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 3.20 Å
1W5C Photosystem II from Thermosynechococcus elongatus Deposited 2004-08-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric
Chain I 1–473（473 aa）
Not recorded CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 MN MANGANESE (II) ION × 4 FE2 FE (II) ION × 1 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 BCR BETA-CAROTENE × 1 HEC HEME C × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 3.20 Å
3KZI Crystal Structure of Monomeric Form of Cyanobacterial Photosystem II Deposited 2009-12-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain C 1–461（461 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 11 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 LMT DODECYL-BETA-D-MALTOSIDE × 7 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 BCT BICARBONATE ION × 1 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；291 K；PEG 400, PIPES, MES, CaCl2, pH 7, batch, temperature 291.0K
Resolution 3.60 Å R-free 0.308
4FBY fs X-ray diffraction of Photosystem II Deposited 2012-05-23 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain C 1–461（461 aa）
Chain P 1–461（461 aa）
Not recorded CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 OEC OXYGEN EVOLVING SYSTEM × 2 FE2 FE (II) ION × 2 BCR BETA-CAROTENE × 24 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 CL CHLORIDE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；298 K；4% PEG 2000, 5 mM CaCl2, 100 mM PIPES, pH 7.0, batch, temperature 298K
Resolution 6.56 Å R-free 0.385
4IXQ RT fs X-ray diffraction of Photosystem II, dark state Deposited 2013-01-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain C 1–461（461 aa）
Chain c 1–461（461 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 LMT DODECYL-BETA-D-MALTOSIDE × 14 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.70 Å R-free 0.314
4IXR RT fs X-ray diffraction of Photosystem II, first illuminated state Deposited 2013-01-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain C 1–461（461 aa）
Chain c 1–461（461 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 LMT DODECYL-BETA-D-MALTOSIDE × 14 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.90 Å R-free 0.313
4PBU Serial Time-resolved crystallography of Photosystem II using a femtosecond X-ray laser The S1 state Deposited 2014-04-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain C 7–461（455 aa）
Chain c 7–461（455 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 CA CALCIUM ION × 7 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 5.00 Å R-free 0.262
4PJ0 Structure of T.elongatus Photosystem II, rows of dimers crystal packing Deposited 2014-05-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain C 1–461（461 aa）
Chain c 1–461（461 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE FE (III) ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SO4 SULFATE ION × 10 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 29 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；pH 7.5；293 K；Batch crystallisation: 2 mM Chl a equivalent of PSIIcc mixed 1:1 with: 0.1 M TRIS (pH 7.5), 0.1 M (NH4)2SO4, 15 - 18 % PEG 5000 MME, post-crystallisation dehydration
Resolution 2.44 Å R-free 0.256
4RVY Serial Time resolved crystallography of Photosystem II using a femtosecond X-ray laser. The S state after two flashes (S3) Deposited 2014-11-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain C 1–461（461 aa）
Chain c 1–461（461 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 CA CALCIUM ION × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions Free interface diffusion；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000 , Free interface diffusion, temperature 283K, temperature 283.0K
Resolution 5.50 Å R-free 0.291
4TNH RT XFEL structure of Photosystem II in the dark state at 4.9 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain C 1–461（461 aa）
Chain c 1–461（461 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.90 Å R-free 0.292
4TNI RT XFEL structure of Photosystem II 500 ms after the third illumination at 4.6 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain C 1–461（461 aa）
Chain c 1–461（461 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 2 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.60 Å R-free 0.284
4TNJ RT XFEL structure of Photosystem II 500 ms after the 2nd illumination (2F) at 4.5 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain C 1–461（461 aa）
Chain c 1–461（461 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.50 Å R-free 0.284
4TNK RT XFEL structure of Photosystem II 250 microsec after the third illumination at 5.2 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain C 1–461（461 aa）
Chain c 1–461（461 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.20 Å R-free 0.289
4V62 Crystal Structure of cyanobacterial Photosystem II Deposited 2008-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain AC 1–461（461 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 3 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 13 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 7 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 CL CHLORIDE ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 7 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG2000, PIPES, magnesium chloride, pH7.0, batch, temperature 298K
Resolution 2.90 Å R-free 0.292
4V62 Crystal Structure of cyanobacterial Photosystem II Deposited 2008-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain BC 1–461（461 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 3 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 11 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 7 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 CL CHLORIDE ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 7 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG2000, PIPES, magnesium chloride, pH7.0, batch, temperature 298K
Resolution 2.90 Å R-free 0.292
4V82 Crystal structure of cyanobacterial Photosystem II in complex with terbutryn Deposited 2010-11-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain AC 1–461（461 aa）
Chain BC 1–461（461 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 70 MST 2-T-BUTYLAMINO-4-ETHYLAMINO-6-METHYLTHIO-S-TRIAZINE × 2 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 LMT DODECYL-BETA-D-MALTOSIDE × 16 DMS DIMETHYL SULFOXIDE × 8 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；Pipes, CaCl2, DMSO, PEG 2000, pH 7.0, batch, temperature 298K
Resolution 3.20 Å R-free 0.299
5E79 Macromolecular diffractive imaging using imperfect crystals Deposited 2015-10-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain C 11–461（451 aa）
Chain c 11–461（451 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 CA CALCIUM ION × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；283.18 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 3.50 Å R-free 0.331
5E7C Macromolecular diffractive imaging using imperfect crystals - Bragg data Deposited 2015-10-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain C 11–461（451 aa）
Chain c 11–461（451 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 FE2 FE (II) ION × 2 CA CALCIUM ION × 6 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 4.50 Å R-free 0.275
5H2F Crystal structure of the PsbM-deletion mutant of photosystem II Deposited 2016-10-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain C 7–461（455 aa） Fragment：UNP RESIDUES 7-461
Not recorded OEX CA-MN4-O5 CLUSTER × 1 FE2 FE (II) ION × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 3 LMT DODECYL-BETA-D-MALTOSIDE × 6 UNL UNKNOWN LIGAND × 13 DMS DIMETHYL SULFOXIDE × 67 BCT BICARBONATE ION × 1 CA CALCIUM ION × 2 HTG heptyl 1-thio-beta-D-glucopyranoside × 9 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6.1；285 K；PEG1450, sodium chloride, calcium chloride, magnesium sulfate
Resolution 2.20 Å R-free 0.226
5H2F Crystal structure of the PsbM-deletion mutant of photosystem II Deposited 2016-10-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain c 7–461（455 aa） Fragment：UNP RESIDUES 7-461
Not recorded OEX CA-MN4-O5 CLUSTER × 1 FE2 FE (II) ION × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 5 LMT DODECYL-BETA-D-MALTOSIDE × 5 UNL UNKNOWN LIGAND × 14 DMS DIMETHYL SULFOXIDE × 66 BCT BICARBONATE ION × 1 CA CALCIUM ION × 3 HTG heptyl 1-thio-beta-D-glucopyranoside × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6.1；285 K；PEG1450, sodium chloride, calcium chloride, magnesium sulfate
Resolution 2.20 Å R-free 0.226
5KAI NH3-bound RT XFEL structure of Photosystem II 500 ms after the 2nd illumination (2F) at 2.8 A resolution Deposited 2016-06-01 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain C 1–461（461 aa）
Chain c 1–461（461 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 UNL UNKNOWN LIGAND × 23 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol
Resolution 2.80 Å R-free 0.300
5MX2 Photosystem II depleted of the Mn4CaO5 cluster at 2.55 A resolution Deposited 2017-01-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain C 1–461（461 aa）
Not recorded CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 11 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LFA EICOSANE × 19 FE FE (III) ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 4 BCT BICARBONATE ION × 1 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 7.5；291 K；equal volumes of buffer: 0.1 M Tris-HCl pH 7.5, 0.1 M (NH4)2SO4, 14-18 % PEG 5000 MME and protein: 2 mM chlorophyll equivalent of PSII in C12E8 detergent, post crystallisation treatment
Resolution 2.20 Å R-free 0.260
5MX2 Photosystem II depleted of the Mn4CaO5 cluster at 2.55 A resolution Deposited 2017-01-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain c 1–461（461 aa）
Not recorded CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 11 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LFA EICOSANE × 20 FE FE (III) ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 4 BCT BICARBONATE ION × 1 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 7.5；291 K；equal volumes of buffer: 0.1 M Tris-HCl pH 7.5, 0.1 M (NH4)2SO4, 14-18 % PEG 5000 MME and protein: 2 mM chlorophyll equivalent of PSII in C12E8 detergent, post crystallisation treatment
Resolution 2.20 Å R-free 0.260
5TIS Room temperature XFEL structure of the native, doubly-illuminated photosystem II complex Deposited 2016-10-03 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain C 1–461（461 aa）
Chain c 1–461（461 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 UNL UNKNOWN LIGAND × 33 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol
Resolution 2.25 Å R-free 0.231
5ZZN Crystal structure of photosystem II from an SQDG-deficient mutant of Thermosynechococcus elongatus Deposited 2018-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain C 7–461（455 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 5 UNL UNKNOWN LIGAND × 27 LMT DODECYL-BETA-D-MALTOSIDE × 9 DMS DIMETHYL SULFOXIDE × 48 CA CALCIUM ION × 3 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 HTG heptyl 1-thio-beta-D-glucopyranoside × 12 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 GOL GLYCEROL × 2 FE2 FE (II) ION × 1 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20MM NACL, 10MM CACL2, 40MM MGSO4, 20MM MES BUFFER, PH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPERATURE 285K
Resolution 2.10 Å R-free 0.197
5ZZN Crystal structure of photosystem II from an SQDG-deficient mutant of Thermosynechococcus elongatus Deposited 2018-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain c 7–461（455 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 1 CL CHLORIDE ION × 3 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 5 UNL UNKNOWN LIGAND × 25 LMT DODECYL-BETA-D-MALTOSIDE × 10 DMS DIMETHYL SULFOXIDE × 47 CA CALCIUM ION × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 HTG heptyl 1-thio-beta-D-glucopyranoside × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 GOL GLYCEROL × 3 FE2 FE (II) ION × 1 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20MM NACL, 10MM CACL2, 40MM MGSO4, 20MM MES BUFFER, PH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPERATURE 285K
Resolution 2.10 Å R-free 0.197
6DHE RT XFEL structure of the dark-stable state of Photosystem II (0F, S1-rich) at 2.05 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain C 11–461（451 aa）
Chain c 11–461（451 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 8CT (6'R,11cis,11'cis,13cis,15cis)-4',5'-didehydro-5',6'-dihydro-beta,beta-carotene × 12 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 BCR BETA-CAROTENE × 10 UNL UNKNOWN LIGAND × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.05 Å R-free 0.241
6DHF RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.08 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain C 11–461（451 aa）
Chain c 11–461（451 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 UNL UNKNOWN LIGAND × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.247
6DHG RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.5 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain C 11–461（451 aa）
Chain c 11–461（451 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.50 Å R-free 0.246
6DHH RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.2 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain C 11–461（451 aa）
Chain c 11–461（451 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.20 Å R-free 0.264
6DHO RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.07 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain C 11–461（451 aa）
Chain c 11–461（451 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.07 Å R-free 0.247
6DHP RT XFEL structure of the three-flash state of Photosystem II (3F, S0-rich) at 2.04 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain C 11–461（451 aa）
Chain c 11–461（451 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 UNL UNKNOWN LIGAND × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.04 Å R-free 0.248
6W1O RT XFEL structure of the dark-stable state of Photosystem II (0F, S1-rich) at 2.08 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain C 1–461（461 aa）
Chain c 1–461（461 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.240
6W1P RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.26 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain C 1–461（461 aa）
Chain c 1–461（461 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.26 Å R-free 0.241
6W1Q RT XFEL structure of Photosystem II 50 microseconds after the second illumination at 2.27 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain C 1–461（461 aa）
Chain c 1–461（461 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 BCR BETA-CAROTENE × 22 STE STEARIC ACID × 35 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 HEC HEME C × 4 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.27 Å R-free 0.249
6W1R RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.23 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain C 1–461（461 aa）
Chain c 1–461（461 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.23 Å R-free 0.235
6W1T RT XFEL structure of Photosystem II 250 microseconds after the second illumination at 2.01 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain C 1–461（461 aa）
Chain c 1–461（461 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.01 Å R-free 0.228
6W1U RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.09 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain C 1–461（461 aa）
Chain c 1–461（461 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
6W1V RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain C 1–461（461 aa）
Chain c 1–461（461 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 STE STEARIC ACID × 38 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 PHO PHEOPHYTIN A × 4 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.241
7NHO Structure of PSII-M Deposited 2021-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain C 1–461（461 aa）
Not recorded FE FE (III) ION × 1 MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 BCT BICARBONATE ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.66 Å
7NHP Structure of PSII-I (PSII with Psb27, Psb28, and Psb34) Deposited 2021-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain C 1–461（461 aa）
Not recorded MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 FE FE (III) ION × 1 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.72 Å
7NHQ Structure of PSII-I prime (PSII with Psb28, and Psb34) Deposited 2021-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 17 PDB declaration: heptadecameric
Chain C 1–461（461 aa）
Not recorded FE FE (III) ION × 1 MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.68 Å
7RF1 RT XFEL structure of Photosystem II averaged across all S-states at 1.89 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain C 1–461（461 aa）
Chain c 1–461（461 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 STE STEARIC ACID × 55 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 1.89 Å R-free 0.214
7RF2 RT XFEL structure of dark-stable state of Photosystem II (0F, S1 rich) at 2.08 Angstrom Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain C 1–461（461 aa）
Chain c 1–461（461 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 33 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.238
7RF3 RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.26 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain C 1–461（461 aa）
Chain c 1–461（461 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.26 Å R-free 0.238
7RF4 RT XFEL structure of Photosystem II 50 microseconds after the second illumination at 2.27 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain C 1–461（461 aa）
Chain c 1–461（461 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 BCR BETA-CAROTENE × 22 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.27 Å R-free 0.244
7RF5 RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.23 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain C 1–461（461 aa）
Chain c 1–461（461 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.23 Å R-free 0.233
7RF6 RT XFEL structure of Photosystem II 250 microseconds after the second illumination at 2.01 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain C 1–461（461 aa）
Chain c 1–461（461 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.01 Å R-free 0.227
7RF7 RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.09 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain C 1–461（461 aa）
Chain c 1–461（461 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
7RF8 RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain C 1–461（461 aa）
Chain c 1–461（461 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
7YQ2 Crystal structure of photosystem II expressing psbA2 gene only Deposited 2022-08-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 39 PDB declaration: 39-meric
Chain C 1–461（461 aa）
Chain c 1–461（461 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 UNL UNKNOWN LIGAND × 77 LMT DODECYL-BETA-D-MALTOSIDE × 19 GOL GLYCEROL × 10 BCT BICARBONATE ION × 3 DMS DIMETHYL SULFOXIDE × 116 CA CALCIUM ION × 7 HTG heptyl 1-thio-beta-D-glucopyranoside × 31 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 9 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 MG MAGNESIUM ION × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20mM NaCl, 10mM CaCl2, 40mM MgSO4, 20mM Mes BUFFER, pH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPARATURE 285K
Resolution 1.90 Å R-free 0.184
7YQ7 Crystal structure of photosystem II expressing psbA3 gene only Deposited 2022-08-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 39 PDB declaration: 39-meric
Chain C 1–461（461 aa）
Chain c 1–461（461 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 UNL UNKNOWN LIGAND × 82 GOL GLYCEROL × 11 LMT DODECYL-BETA-D-MALTOSIDE × 18 BCT BICARBONATE ION × 4 DMS DIMETHYL SULFOXIDE × 71 CA CALCIUM ION × 5 HTG heptyl 1-thio-beta-D-glucopyranoside × 18 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 MG MAGNESIUM ION × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20mM NaCl, 10mM CaCl2, 40mM MgSO4, 20mM MES BUFFER pH 6.0, MICRO-BATCH METHOD UNDER OIL, TMPERATURE 285K
Resolution 1.90 Å R-free 0.177
8EZ5 RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2022-10-31 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain C 1–461（461 aa）
Chain c 1–461（461 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.234
8F4C RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain C 1–461（461 aa）
Chain c 1–461（461 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.224
8F4D RT XFEL structure of Photosystem II 50 microseconds after the third illumination at 2.15 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain C 1–461（461 aa）
Chain c 1–461（461 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.15 Å R-free 0.243
8F4E RT XFEL structure of Photosystem II 250 microseconds after the third illumination at 2.09 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain C 1–461（461 aa）
Chain c 1–461（461 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.233
8F4F RT XFEL structure of Photosystem II 500 microseconds after the third illumination at 2.03 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain C 1–461（461 aa）
Chain c 1–461（461 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.03 Å R-free 0.248
8F4G RT XFEL structure of Photosystem II 730 microseconds after the third illumination at 2.03 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain C 1–461（461 aa）
Chain c 1–461（461 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.03 Å R-free 0.223
8F4H RT XFEL structure of Photosystem II 1200 microseconds after the third illumination at 2.10 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain C 1–461（461 aa）
Chain c 1–461（461 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.10 Å R-free 0.232
8F4I RT XFEL structure of Photosystem II 2000 microseconds after the third illumination at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain C 1–461（461 aa）
Chain c 1–461（461 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.232
8F4J RT XFEL structure of Photosystem II 4000 microseconds after the third illumination at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain C 1–461（461 aa）
Chain c 1–461（461 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.226
8F4K RT XFEL structure of the three-flash state of Photosystem II (3F, S0-rich) at 2.16 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain C 1–461（461 aa）
Chain c 1–461（461 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.16 Å R-free 0.241
9EVX cryoEM structure of Photosystem II averaged across S2-S3 states at 1.71 Angstrom resolution Deposited 2024-04-02 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain C 1–461（461 aa）
Chain c 1–461（461 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 STE STEARIC ACID × 42 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 PHO PHEOPHYTIN A × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；100mM MES pH6.5, 5mM CaCl2, 5% glycerol, 0.03% DDM
cryo-EM vitrification conditions Cryogen ETHANE；The sample-on-grid was illuminated with one and two flashes of light
Resolution 1.71 Å
9G6F Inactive PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain C 1–461（461 aa）
Chain c 1–461（461 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LFA EICOSANE × 33 PLM PALMITIC ACID × 24 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 8 LMT DODECYL-BETA-D-MALTOSIDE × 13 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å
9G6G Semi-active PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric
Chain C 1–461（461 aa）
Chain c 1–461（461 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 3 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 LFA EICOSANE × 30 OEX CA-MN4-O5 CLUSTER × 1 LMT DODECYL-BETA-D-MALTOSIDE × 17 BCT BICARBONATE ION × 2 PLM PALMITIC ACID × 29 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 GOL GLYCEROL × 1 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 3 RRX (3R)-beta,beta-caroten-3-ol × 2 CA CALCIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å
9G6H active PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain C 1–461（461 aa）
Chain c 1–461（461 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 PLM PALMITIC ACID × 35 LFA EICOSANE × 24 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 GOL GLYCEROL × 2 LMT DODECYL-BETA-D-MALTOSIDE × 17 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 RRX (3R)-beta,beta-caroten-3-ol × 2 CA CALCIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å

63 other PDB entries and 69 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PSBC_THEEB
Isoform —
PDB entities 3
Chains and sequence ranges Author chain C； PDBConstruct 1–461； UniProt 1–461 Author chain c； PDBConstruct 1–461； UniProt 1–461

Photosystem II D2 protein

OrganismNot specified

UniProt Q8CM25

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 40 PDB declaration: 40-meric（40） Consistent with protein copy count Chain D； UniProt 1–352 Chain d； UniProt 1–352 Not recorded Photosystem II protein D1 1 × 2 （P0A444） Photosystem II CP47 reaction center protein × 2 （Q8DIQ1） Photosystem II CP43 reaction center protein × 2 （Q8DIF8） Cytochrome b559 subunit alpha × 2 （Q8DIP0） Cytochrome b559 subunit beta × 2 （Q8DIN9） Photosystem II reaction center protein H × 2 （Q8DJ43） Photosystem II reaction center protein I × 2 （Q8DJZ6） Photosystem II reaction center protein J × 2 （P59087） Photosystem II reaction center protein K × 2 （Q9F1K9） Photosystem II reaction center protein L × 2 （Q8DIN8） Photosystem II reaction center protein M × 2 （Q8DHA7） Photosystem II manganese-stabilizing polypeptide × 2 （P0A431） Photosystem II reaction center protein T × 2 （Q8DIQ0） Photosystem II 12 kDa extrinsic protein × 2 （Q9F1L5） Cytochrome c-550 × 2 （P0A386） Photosystem II reaction center protein Ycf12 × 2 （Q8DJI1） Photosystem II reaction center X protein × 2 （Q9F1R6） Photosystem II reaction center protein Z × 2 （Q8DHJ2） Photosystem II protein Y × 2 （Q8DKM3） OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 UNL UNKNOWN LIGAND × 17 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION X-ray crystallization conditions：BATCH MODE；pH 7.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol. Resolution 3.00 Å R-free 0.303

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5KAF Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
11YO Single-conformation model re-refinement of 2F/S3-rich PSII intermediate structure at 2.09 Angstrom resolution Deposited 2026-03-18 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain D 1–352（352 aa）
Chain d 1–352（352 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 38 OEX CA-MN4-O5 CLUSTER × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 PHO PHEOPHYTIN A × 4 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.243
1S5L Architecture of the photosynthetic oxygen evolving center Deposited 2004-01-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain D 1–352（352 aa）
Not recorded FE FE (III) ION × 1 BCT BICARBONATE ION × 2 OEC OXYGEN EVOLVING SYSTEM × 1 CLA CHLOROPHYLL A × 36 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMT DODECYL-BETA-D-MALTOSIDE × 1 BCR BETA-CAROTENE × 7 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pseudo-batch hanging drop；pH 7.5；291 K；100mM HEPES, 100mM (NH4)2SO4, PEG 4000, C12E8, trimethyl lead acetate, pH 7.5, pseudo-batch hanging drop, temperature 291K
Resolution 3.50 Å R-free 0.342
1S5L Architecture of the photosynthetic oxygen evolving center Deposited 2004-01-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain d 1–352（352 aa）
Not recorded FE FE (III) ION × 1 BCT BICARBONATE ION × 2 OEC OXYGEN EVOLVING SYSTEM × 1 CLA CHLOROPHYLL A × 36 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMT DODECYL-BETA-D-MALTOSIDE × 1 BCR BETA-CAROTENE × 7 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pseudo-batch hanging drop；pH 7.5；291 K；100mM HEPES, 100mM (NH4)2SO4, PEG 4000, C12E8, trimethyl lead acetate, pH 7.5, pseudo-batch hanging drop, temperature 291K
Resolution 3.50 Å R-free 0.342
1W5C Photosystem II from Thermosynechococcus elongatus Deposited 2004-08-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric
Chain D 1–352（352 aa）
Not recorded CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 MN MANGANESE (II) ION × 4 FE2 FE (II) ION × 1 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 BCR BETA-CAROTENE × 1 HEC HEME C × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 3.20 Å
1W5C Photosystem II from Thermosynechococcus elongatus Deposited 2004-08-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric
Chain J 1–352（352 aa）
Not recorded CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 MN MANGANESE (II) ION × 4 FE2 FE (II) ION × 1 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 BCR BETA-CAROTENE × 1 HEC HEME C × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 3.20 Å
3KZI Crystal Structure of Monomeric Form of Cyanobacterial Photosystem II Deposited 2009-12-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain D 1–352（352 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 11 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 LMT DODECYL-BETA-D-MALTOSIDE × 7 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 BCT BICARBONATE ION × 1 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；291 K；PEG 400, PIPES, MES, CaCl2, pH 7, batch, temperature 291.0K
Resolution 3.60 Å R-free 0.308
4FBY fs X-ray diffraction of Photosystem II Deposited 2012-05-23 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain D 1–352（352 aa）
Chain Q 1–352（352 aa）
Not recorded CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 OEC OXYGEN EVOLVING SYSTEM × 2 FE2 FE (II) ION × 2 BCR BETA-CAROTENE × 24 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 CL CHLORIDE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；298 K；4% PEG 2000, 5 mM CaCl2, 100 mM PIPES, pH 7.0, batch, temperature 298K
Resolution 6.56 Å R-free 0.385
4IXQ RT fs X-ray diffraction of Photosystem II, dark state Deposited 2013-01-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain D 1–352（352 aa）
Chain d 1–352（352 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 LMT DODECYL-BETA-D-MALTOSIDE × 14 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.70 Å R-free 0.314
4IXR RT fs X-ray diffraction of Photosystem II, first illuminated state Deposited 2013-01-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain D 1–352（352 aa）
Chain d 1–352（352 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 LMT DODECYL-BETA-D-MALTOSIDE × 14 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.90 Å R-free 0.313
4PBU Serial Time-resolved crystallography of Photosystem II using a femtosecond X-ray laser The S1 state Deposited 2014-04-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain D 11–352（342 aa）
Chain d 11–352（342 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 CA CALCIUM ION × 7 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 5.00 Å R-free 0.262
4PJ0 Structure of T.elongatus Photosystem II, rows of dimers crystal packing Deposited 2014-05-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain D 1–352（352 aa）
Chain d 1–352（352 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE FE (III) ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SO4 SULFATE ION × 10 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 29 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；pH 7.5；293 K；Batch crystallisation: 2 mM Chl a equivalent of PSIIcc mixed 1:1 with: 0.1 M TRIS (pH 7.5), 0.1 M (NH4)2SO4, 15 - 18 % PEG 5000 MME, post-crystallisation dehydration
Resolution 2.44 Å R-free 0.256
4RVY Serial Time resolved crystallography of Photosystem II using a femtosecond X-ray laser. The S state after two flashes (S3) Deposited 2014-11-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain D 11–352（342 aa）
Chain d 11–352（342 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 CA CALCIUM ION × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions Free interface diffusion；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000 , Free interface diffusion, temperature 283K, temperature 283.0K
Resolution 5.50 Å R-free 0.291
4TNH RT XFEL structure of Photosystem II in the dark state at 4.9 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain D 1–352（352 aa）
Chain d 1–352（352 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.90 Å R-free 0.292
4TNI RT XFEL structure of Photosystem II 500 ms after the third illumination at 4.6 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain D 1–352（352 aa）
Chain d 1–352（352 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 2 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.60 Å R-free 0.284
4TNJ RT XFEL structure of Photosystem II 500 ms after the 2nd illumination (2F) at 4.5 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain D 1–352（352 aa）
Chain d 1–352（352 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.50 Å R-free 0.284
4TNK RT XFEL structure of Photosystem II 250 microsec after the third illumination at 5.2 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain D 1–352（352 aa）
Chain d 1–352（352 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.20 Å R-free 0.289
4V62 Crystal Structure of cyanobacterial Photosystem II Deposited 2008-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain AD 1–352（352 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 3 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 13 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 7 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 CL CHLORIDE ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 7 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG2000, PIPES, magnesium chloride, pH7.0, batch, temperature 298K
Resolution 2.90 Å R-free 0.292
4V62 Crystal Structure of cyanobacterial Photosystem II Deposited 2008-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain BD 1–352（352 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 3 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 11 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 7 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 CL CHLORIDE ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 7 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG2000, PIPES, magnesium chloride, pH7.0, batch, temperature 298K
Resolution 2.90 Å R-free 0.292
4V82 Crystal structure of cyanobacterial Photosystem II in complex with terbutryn Deposited 2010-11-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain AD 1–352（352 aa）
Chain BD 1–352（352 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 70 MST 2-T-BUTYLAMINO-4-ETHYLAMINO-6-METHYLTHIO-S-TRIAZINE × 2 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 LMT DODECYL-BETA-D-MALTOSIDE × 16 DMS DIMETHYL SULFOXIDE × 8 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；Pipes, CaCl2, DMSO, PEG 2000, pH 7.0, batch, temperature 298K
Resolution 3.20 Å R-free 0.299
5E79 Macromolecular diffractive imaging using imperfect crystals Deposited 2015-10-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain D 11–352（342 aa）
Chain d 11–352（342 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 CA CALCIUM ION × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；283.18 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 3.50 Å R-free 0.331
5E7C Macromolecular diffractive imaging using imperfect crystals - Bragg data Deposited 2015-10-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain D 11–352（342 aa）
Chain d 11–352（342 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 FE2 FE (II) ION × 2 CA CALCIUM ION × 6 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 4.50 Å R-free 0.275
5H2F Crystal structure of the PsbM-deletion mutant of photosystem II Deposited 2016-10-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain D 11–352（342 aa） Fragment：UNP RESIDUES 11-352
Not recorded OEX CA-MN4-O5 CLUSTER × 1 FE2 FE (II) ION × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 3 LMT DODECYL-BETA-D-MALTOSIDE × 6 UNL UNKNOWN LIGAND × 13 DMS DIMETHYL SULFOXIDE × 67 BCT BICARBONATE ION × 1 CA CALCIUM ION × 2 HTG heptyl 1-thio-beta-D-glucopyranoside × 9 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6.1；285 K；PEG1450, sodium chloride, calcium chloride, magnesium sulfate
Resolution 2.20 Å R-free 0.226
5H2F Crystal structure of the PsbM-deletion mutant of photosystem II Deposited 2016-10-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain d 11–352（342 aa） Fragment：UNP RESIDUES 11-352
Not recorded OEX CA-MN4-O5 CLUSTER × 1 FE2 FE (II) ION × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 5 LMT DODECYL-BETA-D-MALTOSIDE × 5 UNL UNKNOWN LIGAND × 14 DMS DIMETHYL SULFOXIDE × 66 BCT BICARBONATE ION × 1 CA CALCIUM ION × 3 HTG heptyl 1-thio-beta-D-glucopyranoside × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6.1；285 K；PEG1450, sodium chloride, calcium chloride, magnesium sulfate
Resolution 2.20 Å R-free 0.226
5KAI NH3-bound RT XFEL structure of Photosystem II 500 ms after the 2nd illumination (2F) at 2.8 A resolution Deposited 2016-06-01 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain D 1–352（352 aa）
Chain d 1–352（352 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 UNL UNKNOWN LIGAND × 23 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol
Resolution 2.80 Å R-free 0.300
5MX2 Photosystem II depleted of the Mn4CaO5 cluster at 2.55 A resolution Deposited 2017-01-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain D 1–352（352 aa）
Not recorded CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 11 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LFA EICOSANE × 19 FE FE (III) ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 4 BCT BICARBONATE ION × 1 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 7.5；291 K；equal volumes of buffer: 0.1 M Tris-HCl pH 7.5, 0.1 M (NH4)2SO4, 14-18 % PEG 5000 MME and protein: 2 mM chlorophyll equivalent of PSII in C12E8 detergent, post crystallisation treatment
Resolution 2.20 Å R-free 0.260
5MX2 Photosystem II depleted of the Mn4CaO5 cluster at 2.55 A resolution Deposited 2017-01-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain d 1–352（352 aa）
Not recorded CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 11 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LFA EICOSANE × 20 FE FE (III) ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 4 BCT BICARBONATE ION × 1 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 7.5；291 K；equal volumes of buffer: 0.1 M Tris-HCl pH 7.5, 0.1 M (NH4)2SO4, 14-18 % PEG 5000 MME and protein: 2 mM chlorophyll equivalent of PSII in C12E8 detergent, post crystallisation treatment
Resolution 2.20 Å R-free 0.260
5TIS Room temperature XFEL structure of the native, doubly-illuminated photosystem II complex Deposited 2016-10-03 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain D 1–352（352 aa）
Chain d 1–352（352 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 UNL UNKNOWN LIGAND × 33 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol
Resolution 2.25 Å R-free 0.231
5ZZN Crystal structure of photosystem II from an SQDG-deficient mutant of Thermosynechococcus elongatus Deposited 2018-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain D 11–352（342 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) OEX CA-MN4-O5 CLUSTER × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 5 UNL UNKNOWN LIGAND × 27 LMT DODECYL-BETA-D-MALTOSIDE × 9 DMS DIMETHYL SULFOXIDE × 48 CA CALCIUM ION × 3 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 HTG heptyl 1-thio-beta-D-glucopyranoside × 12 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 GOL GLYCEROL × 2 FE2 FE (II) ION × 1 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20MM NACL, 10MM CACL2, 40MM MGSO4, 20MM MES BUFFER, PH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPERATURE 285K
Resolution 2.10 Å R-free 0.197
5ZZN Crystal structure of photosystem II from an SQDG-deficient mutant of Thermosynechococcus elongatus Deposited 2018-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain d 11–352（342 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) OEX CA-MN4-O5 CLUSTER × 1 CL CHLORIDE ION × 3 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 5 UNL UNKNOWN LIGAND × 25 LMT DODECYL-BETA-D-MALTOSIDE × 10 DMS DIMETHYL SULFOXIDE × 47 CA CALCIUM ION × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 HTG heptyl 1-thio-beta-D-glucopyranoside × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 GOL GLYCEROL × 3 FE2 FE (II) ION × 1 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20MM NACL, 10MM CACL2, 40MM MGSO4, 20MM MES BUFFER, PH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPERATURE 285K
Resolution 2.10 Å R-free 0.197
6DHE RT XFEL structure of the dark-stable state of Photosystem II (0F, S1-rich) at 2.05 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain D 12–352（341 aa）
Chain d 12–352（341 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 8CT (6'R,11cis,11'cis,13cis,15cis)-4',5'-didehydro-5',6'-dihydro-beta,beta-carotene × 12 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 BCR BETA-CAROTENE × 10 UNL UNKNOWN LIGAND × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.05 Å R-free 0.241
6DHF RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.08 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain D 12–352（341 aa）
Chain d 12–352（341 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 UNL UNKNOWN LIGAND × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.247
6DHG RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.5 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain D 12–352（341 aa）
Chain d 12–352（341 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.50 Å R-free 0.246
6DHH RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.2 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain D 12–352（341 aa）
Chain d 12–352（341 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.20 Å R-free 0.264
6DHO RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.07 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain D 12–352（341 aa）
Chain d 12–352（341 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.07 Å R-free 0.247
6DHP RT XFEL structure of the three-flash state of Photosystem II (3F, S0-rich) at 2.04 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain D 12–352（341 aa）
Chain d 12–352（341 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 UNL UNKNOWN LIGAND × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.04 Å R-free 0.248
6W1O RT XFEL structure of the dark-stable state of Photosystem II (0F, S1-rich) at 2.08 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain D 1–352（352 aa）
Chain d 1–352（352 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.240
6W1P RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.26 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain D 1–352（352 aa）
Chain d 1–352（352 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.26 Å R-free 0.241
6W1Q RT XFEL structure of Photosystem II 50 microseconds after the second illumination at 2.27 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain D 1–352（352 aa）
Chain d 1–352（352 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 BCR BETA-CAROTENE × 22 STE STEARIC ACID × 35 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 HEC HEME C × 4 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.27 Å R-free 0.249
6W1R RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.23 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain D 1–352（352 aa）
Chain d 1–352（352 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.23 Å R-free 0.235
6W1T RT XFEL structure of Photosystem II 250 microseconds after the second illumination at 2.01 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain D 1–352（352 aa）
Chain d 1–352（352 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.01 Å R-free 0.228
6W1U RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.09 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain D 1–352（352 aa）
Chain d 1–352（352 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
6W1V RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain D 1–352（352 aa）
Chain d 1–352（352 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 STE STEARIC ACID × 38 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 PHO PHEOPHYTIN A × 4 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.241
7NHO Structure of PSII-M Deposited 2021-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain D 1–352（352 aa）
Not recorded FE FE (III) ION × 1 MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 BCT BICARBONATE ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.66 Å
7NHP Structure of PSII-I (PSII with Psb27, Psb28, and Psb34) Deposited 2021-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain D 1–352（352 aa）
Not recorded MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 FE FE (III) ION × 1 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.72 Å
7NHQ Structure of PSII-I prime (PSII with Psb28, and Psb34) Deposited 2021-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 17 PDB declaration: heptadecameric
Chain D 1–352（352 aa）
Not recorded FE FE (III) ION × 1 MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.68 Å
7RF1 RT XFEL structure of Photosystem II averaged across all S-states at 1.89 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain D 1–352（352 aa）
Chain d 1–352（352 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 STE STEARIC ACID × 55 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 1.89 Å R-free 0.214
7RF2 RT XFEL structure of dark-stable state of Photosystem II (0F, S1 rich) at 2.08 Angstrom Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain D 1–352（352 aa）
Chain d 1–352（352 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 33 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.238
7RF3 RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.26 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain D 1–352（352 aa）
Chain d 1–352（352 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.26 Å R-free 0.238
7RF4 RT XFEL structure of Photosystem II 50 microseconds after the second illumination at 2.27 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain D 1–352（352 aa）
Chain d 1–352（352 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 BCR BETA-CAROTENE × 22 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.27 Å R-free 0.244
7RF5 RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.23 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain D 1–352（352 aa）
Chain d 1–352（352 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.23 Å R-free 0.233
7RF6 RT XFEL structure of Photosystem II 250 microseconds after the second illumination at 2.01 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain D 1–352（352 aa）
Chain d 1–352（352 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.01 Å R-free 0.227
7RF7 RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.09 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain D 1–352（352 aa）
Chain d 1–352（352 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
7RF8 RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain D 1–352（352 aa）
Chain d 1–352（352 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
7YQ2 Crystal structure of photosystem II expressing psbA2 gene only Deposited 2022-08-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 39 PDB declaration: 39-meric
Chain D 1–352（352 aa）
Chain d 1–352（352 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 UNL UNKNOWN LIGAND × 77 LMT DODECYL-BETA-D-MALTOSIDE × 19 GOL GLYCEROL × 10 BCT BICARBONATE ION × 3 DMS DIMETHYL SULFOXIDE × 116 CA CALCIUM ION × 7 HTG heptyl 1-thio-beta-D-glucopyranoside × 31 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 9 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 MG MAGNESIUM ION × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20mM NaCl, 10mM CaCl2, 40mM MgSO4, 20mM Mes BUFFER, pH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPARATURE 285K
Resolution 1.90 Å R-free 0.184
7YQ7 Crystal structure of photosystem II expressing psbA3 gene only Deposited 2022-08-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 39 PDB declaration: 39-meric
Chain D 1–352（352 aa）
Chain d 1–352（352 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 UNL UNKNOWN LIGAND × 82 GOL GLYCEROL × 11 LMT DODECYL-BETA-D-MALTOSIDE × 18 BCT BICARBONATE ION × 4 DMS DIMETHYL SULFOXIDE × 71 CA CALCIUM ION × 5 HTG heptyl 1-thio-beta-D-glucopyranoside × 18 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 MG MAGNESIUM ION × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20mM NaCl, 10mM CaCl2, 40mM MgSO4, 20mM MES BUFFER pH 6.0, MICRO-BATCH METHOD UNDER OIL, TMPERATURE 285K
Resolution 1.90 Å R-free 0.177
8EZ5 RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2022-10-31 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain D 1–352（352 aa）
Chain d 1–352（352 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.234
8F4C RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain D 1–352（352 aa）
Chain d 1–352（352 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.224
8F4D RT XFEL structure of Photosystem II 50 microseconds after the third illumination at 2.15 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain D 1–352（352 aa）
Chain d 1–352（352 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.15 Å R-free 0.243
8F4E RT XFEL structure of Photosystem II 250 microseconds after the third illumination at 2.09 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain D 1–352（352 aa）
Chain d 1–352（352 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.233
8F4F RT XFEL structure of Photosystem II 500 microseconds after the third illumination at 2.03 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain D 1–352（352 aa）
Chain d 1–352（352 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.03 Å R-free 0.248
8F4G RT XFEL structure of Photosystem II 730 microseconds after the third illumination at 2.03 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain D 1–352（352 aa）
Chain d 1–352（352 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.03 Å R-free 0.223
8F4H RT XFEL structure of Photosystem II 1200 microseconds after the third illumination at 2.10 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain D 1–352（352 aa）
Chain d 1–352（352 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.10 Å R-free 0.232
8F4I RT XFEL structure of Photosystem II 2000 microseconds after the third illumination at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain D 1–352（352 aa）
Chain d 1–352（352 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.232
8F4J RT XFEL structure of Photosystem II 4000 microseconds after the third illumination at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain D 1–352（352 aa）
Chain d 1–352（352 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.226
8F4K RT XFEL structure of the three-flash state of Photosystem II (3F, S0-rich) at 2.16 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain D 1–352（352 aa）
Chain d 1–352（352 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.16 Å R-free 0.241
9EVX cryoEM structure of Photosystem II averaged across S2-S3 states at 1.71 Angstrom resolution Deposited 2024-04-02 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain D 1–352（352 aa）
Chain d 1–352（352 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 STE STEARIC ACID × 42 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 PHO PHEOPHYTIN A × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；100mM MES pH6.5, 5mM CaCl2, 5% glycerol, 0.03% DDM
cryo-EM vitrification conditions Cryogen ETHANE；The sample-on-grid was illuminated with one and two flashes of light
Resolution 1.71 Å
9G6F Inactive PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain D 1–352（352 aa）
Chain d 1–352（352 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LFA EICOSANE × 33 PLM PALMITIC ACID × 24 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 8 LMT DODECYL-BETA-D-MALTOSIDE × 13 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å
9G6G Semi-active PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric
Chain D 1–352（352 aa）
Chain d 1–352（352 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 3 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 LFA EICOSANE × 30 OEX CA-MN4-O5 CLUSTER × 1 LMT DODECYL-BETA-D-MALTOSIDE × 17 BCT BICARBONATE ION × 2 PLM PALMITIC ACID × 29 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 GOL GLYCEROL × 1 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 3 RRX (3R)-beta,beta-caroten-3-ol × 2 CA CALCIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å
9G6H active PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain D 1–352（352 aa）
Chain d 1–352（352 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 PLM PALMITIC ACID × 35 LFA EICOSANE × 24 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 GOL GLYCEROL × 2 LMT DODECYL-BETA-D-MALTOSIDE × 17 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 RRX (3R)-beta,beta-caroten-3-ol × 2 CA CALCIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å

63 other PDB entries and 69 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PSBD_THEEB
Isoform —
PDB entities 4
Chains and sequence ranges Author chain D； PDBConstruct 1–352； UniProt 1–352 Author chain d； PDBConstruct 1–352； UniProt 1–352

Cytochrome b559 subunit alpha

OrganismNot specified

UniProt Q8DIP0

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 40 PDB declaration: 40-meric（40） Consistent with protein copy count Chain E； UniProt 1–84 Chain e； UniProt 1–84 Not recorded Photosystem II protein D1 1 × 2 （P0A444） Photosystem II CP47 reaction center protein × 2 （Q8DIQ1） Photosystem II CP43 reaction center protein × 2 （Q8DIF8） Photosystem II D2 protein × 2 （Q8CM25） Cytochrome b559 subunit beta × 2 （Q8DIN9） Photosystem II reaction center protein H × 2 （Q8DJ43） Photosystem II reaction center protein I × 2 （Q8DJZ6） Photosystem II reaction center protein J × 2 （P59087） Photosystem II reaction center protein K × 2 （Q9F1K9） Photosystem II reaction center protein L × 2 （Q8DIN8） Photosystem II reaction center protein M × 2 （Q8DHA7） Photosystem II manganese-stabilizing polypeptide × 2 （P0A431） Photosystem II reaction center protein T × 2 （Q8DIQ0） Photosystem II 12 kDa extrinsic protein × 2 （Q9F1L5） Cytochrome c-550 × 2 （P0A386） Photosystem II reaction center protein Ycf12 × 2 （Q8DJI1） Photosystem II reaction center X protein × 2 （Q9F1R6） Photosystem II reaction center protein Z × 2 （Q8DHJ2） Photosystem II protein Y × 2 （Q8DKM3） OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 UNL UNKNOWN LIGAND × 17 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION X-ray crystallization conditions：BATCH MODE；pH 7.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol. Resolution 3.00 Å R-free 0.303

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5KAF Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
11YO Single-conformation model re-refinement of 2F/S3-rich PSII intermediate structure at 2.09 Angstrom resolution Deposited 2026-03-18 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain E 1–84（84 aa）
Chain e 1–84（84 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 38 OEX CA-MN4-O5 CLUSTER × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 PHO PHEOPHYTIN A × 4 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.243
1S5L Architecture of the photosynthetic oxygen evolving center Deposited 2004-01-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain E 1–84（84 aa）
Not recorded FE FE (III) ION × 1 BCT BICARBONATE ION × 2 OEC OXYGEN EVOLVING SYSTEM × 1 CLA CHLOROPHYLL A × 36 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMT DODECYL-BETA-D-MALTOSIDE × 1 BCR BETA-CAROTENE × 7 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pseudo-batch hanging drop；pH 7.5；291 K；100mM HEPES, 100mM (NH4)2SO4, PEG 4000, C12E8, trimethyl lead acetate, pH 7.5, pseudo-batch hanging drop, temperature 291K
Resolution 3.50 Å R-free 0.342
1S5L Architecture of the photosynthetic oxygen evolving center Deposited 2004-01-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain e 1–84（84 aa）
Not recorded FE FE (III) ION × 1 BCT BICARBONATE ION × 2 OEC OXYGEN EVOLVING SYSTEM × 1 CLA CHLOROPHYLL A × 36 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMT DODECYL-BETA-D-MALTOSIDE × 1 BCR BETA-CAROTENE × 7 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pseudo-batch hanging drop；pH 7.5；291 K；100mM HEPES, 100mM (NH4)2SO4, PEG 4000, C12E8, trimethyl lead acetate, pH 7.5, pseudo-batch hanging drop, temperature 291K
Resolution 3.50 Å R-free 0.342
2AXT Crystal Structure of Photosystem II from Thermosynechococcus elongatus Deposited 2005-09-06 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 36 PDB declaration: 36-meric
Chain E 0–83（84 aa）
Chain e 0–83（84 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PQ9 5-[(2E,6E,10E,14E,18E,22E)-3,7,11,15,19,23,27-HEPTAMETHYLOCTACOSA-2,6,10,14,18,22,26-HEPTAENYL]-2,3-DIMETHYLBENZO-1,4-QUINONE × 4 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 22 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LMT DODECYL-BETA-D-MALTOSIDE × 6 MGE (1S)-2-(ALPHA-L-ALLOPYRANOSYLOXY)-1-[(TRIDECANOYLOXY)METHYL]ETHYL PALMITATE × 12 UNL UNKNOWN LIGAND × 34 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG 2000, PIPES, magnesium chloride, pH 7.0, batch, temperature 298K
Resolution 3.00 Å R-free 0.286
3KZI Crystal Structure of Monomeric Form of Cyanobacterial Photosystem II Deposited 2009-12-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain E 2–84（83 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 11 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 LMT DODECYL-BETA-D-MALTOSIDE × 7 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 BCT BICARBONATE ION × 1 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；291 K；PEG 400, PIPES, MES, CaCl2, pH 7, batch, temperature 291.0K
Resolution 3.60 Å R-free 0.308
4FBY fs X-ray diffraction of Photosystem II Deposited 2012-05-23 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain E 2–84（83 aa）
Chain R 2–84（83 aa）
Not recorded CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 OEC OXYGEN EVOLVING SYSTEM × 2 FE2 FE (II) ION × 2 BCR BETA-CAROTENE × 24 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 CL CHLORIDE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；298 K；4% PEG 2000, 5 mM CaCl2, 100 mM PIPES, pH 7.0, batch, temperature 298K
Resolution 6.56 Å R-free 0.385
4IXQ RT fs X-ray diffraction of Photosystem II, dark state Deposited 2013-01-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain E 1–84（84 aa）
Chain e 1–84（84 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 LMT DODECYL-BETA-D-MALTOSIDE × 14 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.70 Å R-free 0.314
4IXR RT fs X-ray diffraction of Photosystem II, first illuminated state Deposited 2013-01-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain E 1–84（84 aa）
Chain e 1–84（84 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 LMT DODECYL-BETA-D-MALTOSIDE × 14 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.90 Å R-free 0.313
4PBU Serial Time-resolved crystallography of Photosystem II using a femtosecond X-ray laser The S1 state Deposited 2014-04-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain E 4–84（81 aa）
Chain e 4–84（81 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 CA CALCIUM ION × 7 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 5.00 Å R-free 0.262
4PJ0 Structure of T.elongatus Photosystem II, rows of dimers crystal packing Deposited 2014-05-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain E 1–84（84 aa）
Chain e 1–84（84 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE FE (III) ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SO4 SULFATE ION × 10 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 29 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；pH 7.5；293 K；Batch crystallisation: 2 mM Chl a equivalent of PSIIcc mixed 1:1 with: 0.1 M TRIS (pH 7.5), 0.1 M (NH4)2SO4, 15 - 18 % PEG 5000 MME, post-crystallisation dehydration
Resolution 2.44 Å R-free 0.256
4RVY Serial Time resolved crystallography of Photosystem II using a femtosecond X-ray laser. The S state after two flashes (S3) Deposited 2014-11-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain E 4–84（81 aa）
Chain e 4–84（81 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 CA CALCIUM ION × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions Free interface diffusion；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000 , Free interface diffusion, temperature 283K, temperature 283.0K
Resolution 5.50 Å R-free 0.291
4TNH RT XFEL structure of Photosystem II in the dark state at 4.9 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain E 1–84（84 aa）
Chain e 1–84（84 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.90 Å R-free 0.292
4TNI RT XFEL structure of Photosystem II 500 ms after the third illumination at 4.6 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain E 1–84（84 aa）
Chain e 1–84（84 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 2 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.60 Å R-free 0.284
4TNJ RT XFEL structure of Photosystem II 500 ms after the 2nd illumination (2F) at 4.5 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain E 1–84（84 aa）
Chain e 1–84（84 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.50 Å R-free 0.284
4TNK RT XFEL structure of Photosystem II 250 microsec after the third illumination at 5.2 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain E 1–84（84 aa）
Chain e 1–84（84 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.20 Å R-free 0.289
4V62 Crystal Structure of cyanobacterial Photosystem II Deposited 2008-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain AE 1–84（84 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 3 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 13 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 7 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 CL CHLORIDE ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 7 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG2000, PIPES, magnesium chloride, pH7.0, batch, temperature 298K
Resolution 2.90 Å R-free 0.292
4V62 Crystal Structure of cyanobacterial Photosystem II Deposited 2008-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain BE 1–84（84 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 3 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 11 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 7 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 CL CHLORIDE ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 7 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG2000, PIPES, magnesium chloride, pH7.0, batch, temperature 298K
Resolution 2.90 Å R-free 0.292
4V82 Crystal structure of cyanobacterial Photosystem II in complex with terbutryn Deposited 2010-11-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain AE 1–84（84 aa）
Chain BE 1–84（84 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 70 MST 2-T-BUTYLAMINO-4-ETHYLAMINO-6-METHYLTHIO-S-TRIAZINE × 2 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 LMT DODECYL-BETA-D-MALTOSIDE × 16 DMS DIMETHYL SULFOXIDE × 8 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；Pipes, CaCl2, DMSO, PEG 2000, pH 7.0, batch, temperature 298K
Resolution 3.20 Å R-free 0.299
5E79 Macromolecular diffractive imaging using imperfect crystals Deposited 2015-10-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain E 4–84（81 aa）
Chain e 4–84（81 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 CA CALCIUM ION × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；283.18 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 3.50 Å R-free 0.331
5E7C Macromolecular diffractive imaging using imperfect crystals - Bragg data Deposited 2015-10-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain E 4–84（81 aa）
Chain e 4–84（81 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 FE2 FE (II) ION × 2 CA CALCIUM ION × 6 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 4.50 Å R-free 0.275
5H2F Crystal structure of the PsbM-deletion mutant of photosystem II Deposited 2016-10-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain E 5–84（80 aa） Fragment：UNP RESIDUES 5-84
Not recorded OEX CA-MN4-O5 CLUSTER × 1 FE2 FE (II) ION × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 3 LMT DODECYL-BETA-D-MALTOSIDE × 6 UNL UNKNOWN LIGAND × 13 DMS DIMETHYL SULFOXIDE × 67 BCT BICARBONATE ION × 1 CA CALCIUM ION × 2 HTG heptyl 1-thio-beta-D-glucopyranoside × 9 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6.1；285 K；PEG1450, sodium chloride, calcium chloride, magnesium sulfate
Resolution 2.20 Å R-free 0.226
5H2F Crystal structure of the PsbM-deletion mutant of photosystem II Deposited 2016-10-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain e 5–84（80 aa） Fragment：UNP RESIDUES 5-84
Not recorded OEX CA-MN4-O5 CLUSTER × 1 FE2 FE (II) ION × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 5 LMT DODECYL-BETA-D-MALTOSIDE × 5 UNL UNKNOWN LIGAND × 14 DMS DIMETHYL SULFOXIDE × 66 BCT BICARBONATE ION × 1 CA CALCIUM ION × 3 HTG heptyl 1-thio-beta-D-glucopyranoside × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6.1；285 K；PEG1450, sodium chloride, calcium chloride, magnesium sulfate
Resolution 2.20 Å R-free 0.226
5KAI NH3-bound RT XFEL structure of Photosystem II 500 ms after the 2nd illumination (2F) at 2.8 A resolution Deposited 2016-06-01 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain E 1–84（84 aa）
Chain e 1–84（84 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 UNL UNKNOWN LIGAND × 23 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol
Resolution 2.80 Å R-free 0.300
5MX2 Photosystem II depleted of the Mn4CaO5 cluster at 2.55 A resolution Deposited 2017-01-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain E 1–84（84 aa）
Not recorded CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 11 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LFA EICOSANE × 19 FE FE (III) ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 4 BCT BICARBONATE ION × 1 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 7.5；291 K；equal volumes of buffer: 0.1 M Tris-HCl pH 7.5, 0.1 M (NH4)2SO4, 14-18 % PEG 5000 MME and protein: 2 mM chlorophyll equivalent of PSII in C12E8 detergent, post crystallisation treatment
Resolution 2.20 Å R-free 0.260
5MX2 Photosystem II depleted of the Mn4CaO5 cluster at 2.55 A resolution Deposited 2017-01-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain e 1–84（84 aa）
Not recorded CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 11 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LFA EICOSANE × 20 FE FE (III) ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 4 BCT BICARBONATE ION × 1 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 7.5；291 K；equal volumes of buffer: 0.1 M Tris-HCl pH 7.5, 0.1 M (NH4)2SO4, 14-18 % PEG 5000 MME and protein: 2 mM chlorophyll equivalent of PSII in C12E8 detergent, post crystallisation treatment
Resolution 2.20 Å R-free 0.260
5TIS Room temperature XFEL structure of the native, doubly-illuminated photosystem II complex Deposited 2016-10-03 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain E 1–84（84 aa）
Chain e 1–84（84 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 UNL UNKNOWN LIGAND × 33 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol
Resolution 2.25 Å R-free 0.231
5ZZN Crystal structure of photosystem II from an SQDG-deficient mutant of Thermosynechococcus elongatus Deposited 2018-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain E 4–84（81 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 5 UNL UNKNOWN LIGAND × 27 LMT DODECYL-BETA-D-MALTOSIDE × 9 DMS DIMETHYL SULFOXIDE × 48 CA CALCIUM ION × 3 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 HTG heptyl 1-thio-beta-D-glucopyranoside × 12 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 GOL GLYCEROL × 2 FE2 FE (II) ION × 1 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20MM NACL, 10MM CACL2, 40MM MGSO4, 20MM MES BUFFER, PH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPERATURE 285K
Resolution 2.10 Å R-free 0.197
5ZZN Crystal structure of photosystem II from an SQDG-deficient mutant of Thermosynechococcus elongatus Deposited 2018-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain e 4–84（81 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 1 CL CHLORIDE ION × 3 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 5 UNL UNKNOWN LIGAND × 25 LMT DODECYL-BETA-D-MALTOSIDE × 10 DMS DIMETHYL SULFOXIDE × 47 CA CALCIUM ION × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 HTG heptyl 1-thio-beta-D-glucopyranoside × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 GOL GLYCEROL × 3 FE2 FE (II) ION × 1 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20MM NACL, 10MM CACL2, 40MM MGSO4, 20MM MES BUFFER, PH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPERATURE 285K
Resolution 2.10 Å R-free 0.197
6DHE RT XFEL structure of the dark-stable state of Photosystem II (0F, S1-rich) at 2.05 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain E 3–84（82 aa）
Chain e 3–84（82 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 8CT (6'R,11cis,11'cis,13cis,15cis)-4',5'-didehydro-5',6'-dihydro-beta,beta-carotene × 12 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 BCR BETA-CAROTENE × 10 UNL UNKNOWN LIGAND × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.05 Å R-free 0.241
6DHF RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.08 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain E 3–84（82 aa）
Chain e 3–84（82 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 UNL UNKNOWN LIGAND × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.247
6DHG RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.5 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain E 3–84（82 aa）
Chain e 3–84（82 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.50 Å R-free 0.246
6DHH RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.2 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain E 3–84（82 aa）
Chain e 3–84（82 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.20 Å R-free 0.264
6DHO RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.07 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain E 3–84（82 aa）
Chain e 3–84（82 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.07 Å R-free 0.247
6DHP RT XFEL structure of the three-flash state of Photosystem II (3F, S0-rich) at 2.04 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain E 3–84（82 aa）
Chain e 3–84（82 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 UNL UNKNOWN LIGAND × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.04 Å R-free 0.248
6W1O RT XFEL structure of the dark-stable state of Photosystem II (0F, S1-rich) at 2.08 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain E 1–84（84 aa）
Chain e 1–84（84 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.240
6W1P RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.26 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain E 1–84（84 aa）
Chain e 1–84（84 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.26 Å R-free 0.241
6W1Q RT XFEL structure of Photosystem II 50 microseconds after the second illumination at 2.27 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain E 1–84（84 aa）
Chain e 1–84（84 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 BCR BETA-CAROTENE × 22 STE STEARIC ACID × 35 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 HEC HEME C × 4 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.27 Å R-free 0.249
6W1R RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.23 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain E 1–84（84 aa）
Chain e 1–84（84 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.23 Å R-free 0.235
6W1T RT XFEL structure of Photosystem II 250 microseconds after the second illumination at 2.01 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain E 1–84（84 aa）
Chain e 1–84（84 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.01 Å R-free 0.228
6W1U RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.09 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain E 1–84（84 aa）
Chain e 1–84（84 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
6W1V RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain E 1–84（84 aa）
Chain e 1–84（84 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 STE STEARIC ACID × 38 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 PHO PHEOPHYTIN A × 4 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.241
7NHO Structure of PSII-M Deposited 2021-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain E 1–84（84 aa）
Not recorded FE FE (III) ION × 1 MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 BCT BICARBONATE ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.66 Å
7NHP Structure of PSII-I (PSII with Psb27, Psb28, and Psb34) Deposited 2021-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain E 1–84（84 aa）
Not recorded MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 FE FE (III) ION × 1 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.72 Å
7NHQ Structure of PSII-I prime (PSII with Psb28, and Psb34) Deposited 2021-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 17 PDB declaration: heptadecameric
Chain E 1–84（84 aa）
Not recorded FE FE (III) ION × 1 MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.68 Å
7RF1 RT XFEL structure of Photosystem II averaged across all S-states at 1.89 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain E 1–84（84 aa）
Chain e 1–84（84 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 STE STEARIC ACID × 55 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 1.89 Å R-free 0.214
7RF2 RT XFEL structure of dark-stable state of Photosystem II (0F, S1 rich) at 2.08 Angstrom Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain E 1–84（84 aa）
Chain e 1–84（84 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 33 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.238
7RF3 RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.26 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain E 1–84（84 aa）
Chain e 1–84（84 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.26 Å R-free 0.238
7RF4 RT XFEL structure of Photosystem II 50 microseconds after the second illumination at 2.27 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain E 1–84（84 aa）
Chain e 1–84（84 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 BCR BETA-CAROTENE × 22 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.27 Å R-free 0.244
7RF5 RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.23 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain E 1–84（84 aa）
Chain e 1–84（84 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.23 Å R-free 0.233
7RF6 RT XFEL structure of Photosystem II 250 microseconds after the second illumination at 2.01 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain E 1–84（84 aa）
Chain e 1–84（84 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.01 Å R-free 0.227
7RF7 RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.09 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain E 1–84（84 aa）
Chain e 1–84（84 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
7RF8 RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain E 1–84（84 aa）
Chain e 1–84（84 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
7YQ2 Crystal structure of photosystem II expressing psbA2 gene only Deposited 2022-08-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 39 PDB declaration: 39-meric
Chain E 1–84（84 aa）
Chain e 1–84（84 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 UNL UNKNOWN LIGAND × 77 LMT DODECYL-BETA-D-MALTOSIDE × 19 GOL GLYCEROL × 10 BCT BICARBONATE ION × 3 DMS DIMETHYL SULFOXIDE × 116 CA CALCIUM ION × 7 HTG heptyl 1-thio-beta-D-glucopyranoside × 31 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 9 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 MG MAGNESIUM ION × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20mM NaCl, 10mM CaCl2, 40mM MgSO4, 20mM Mes BUFFER, pH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPARATURE 285K
Resolution 1.90 Å R-free 0.184
7YQ7 Crystal structure of photosystem II expressing psbA3 gene only Deposited 2022-08-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 39 PDB declaration: 39-meric
Chain E 1–84（84 aa）
Chain e 1–84（84 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 UNL UNKNOWN LIGAND × 82 GOL GLYCEROL × 11 LMT DODECYL-BETA-D-MALTOSIDE × 18 BCT BICARBONATE ION × 4 DMS DIMETHYL SULFOXIDE × 71 CA CALCIUM ION × 5 HTG heptyl 1-thio-beta-D-glucopyranoside × 18 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 MG MAGNESIUM ION × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20mM NaCl, 10mM CaCl2, 40mM MgSO4, 20mM MES BUFFER pH 6.0, MICRO-BATCH METHOD UNDER OIL, TMPERATURE 285K
Resolution 1.90 Å R-free 0.177
8EZ5 RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2022-10-31 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain E 1–84（84 aa）
Chain e 1–84（84 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.234
8F4C RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain E 1–84（84 aa）
Chain e 1–84（84 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.224
8F4D RT XFEL structure of Photosystem II 50 microseconds after the third illumination at 2.15 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain E 1–84（84 aa）
Chain e 1–84（84 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.15 Å R-free 0.243
8F4E RT XFEL structure of Photosystem II 250 microseconds after the third illumination at 2.09 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain E 1–84（84 aa）
Chain e 1–84（84 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.233
8F4F RT XFEL structure of Photosystem II 500 microseconds after the third illumination at 2.03 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain E 1–84（84 aa）
Chain e 1–84（84 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.03 Å R-free 0.248
8F4G RT XFEL structure of Photosystem II 730 microseconds after the third illumination at 2.03 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain E 1–84（84 aa）
Chain e 1–84（84 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.03 Å R-free 0.223
8F4H RT XFEL structure of Photosystem II 1200 microseconds after the third illumination at 2.10 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain E 1–84（84 aa）
Chain e 1–84（84 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.10 Å R-free 0.232
8F4I RT XFEL structure of Photosystem II 2000 microseconds after the third illumination at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain E 1–84（84 aa）
Chain e 1–84（84 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.232
8F4J RT XFEL structure of Photosystem II 4000 microseconds after the third illumination at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain E 1–84（84 aa）
Chain e 1–84（84 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.226
8F4K RT XFEL structure of the three-flash state of Photosystem II (3F, S0-rich) at 2.16 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain E 1–84（84 aa）
Chain e 1–84（84 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.16 Å R-free 0.241
9EVX cryoEM structure of Photosystem II averaged across S2-S3 states at 1.71 Angstrom resolution Deposited 2024-04-02 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain E 1–84（84 aa）
Chain e 1–84（84 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 STE STEARIC ACID × 42 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 PHO PHEOPHYTIN A × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；100mM MES pH6.5, 5mM CaCl2, 5% glycerol, 0.03% DDM
cryo-EM vitrification conditions Cryogen ETHANE；The sample-on-grid was illuminated with one and two flashes of light
Resolution 1.71 Å
9G6F Inactive PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain E 1–84（84 aa）
Chain e 1–84（84 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LFA EICOSANE × 33 PLM PALMITIC ACID × 24 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 8 LMT DODECYL-BETA-D-MALTOSIDE × 13 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å
9G6G Semi-active PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric
Chain E 1–84（84 aa）
Chain e 1–84（84 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 3 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 LFA EICOSANE × 30 OEX CA-MN4-O5 CLUSTER × 1 LMT DODECYL-BETA-D-MALTOSIDE × 17 BCT BICARBONATE ION × 2 PLM PALMITIC ACID × 29 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 GOL GLYCEROL × 1 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 3 RRX (3R)-beta,beta-caroten-3-ol × 2 CA CALCIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å
9G6H active PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain E 1–84（84 aa）
Chain e 1–84（84 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 PLM PALMITIC ACID × 35 LFA EICOSANE × 24 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 GOL GLYCEROL × 2 LMT DODECYL-BETA-D-MALTOSIDE × 17 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 RRX (3R)-beta,beta-caroten-3-ol × 2 CA CALCIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å

63 other PDB entries and 68 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PSBE_THEEB
Isoform —
PDB entities 5
Chains and sequence ranges Author chain E； PDBConstruct 1–84； UniProt 1–84 Author chain e； PDBConstruct 1–84； UniProt 1–84

Cytochrome b559 subunit beta

OrganismNot specified

UniProt Q8DIN9

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 40 PDB declaration: 40-meric（40） Consistent with protein copy count Chain F； UniProt 1–45 Chain f； UniProt 1–45 Not recorded Photosystem II protein D1 1 × 2 （P0A444） Photosystem II CP47 reaction center protein × 2 （Q8DIQ1） Photosystem II CP43 reaction center protein × 2 （Q8DIF8） Photosystem II D2 protein × 2 （Q8CM25） Cytochrome b559 subunit alpha × 2 （Q8DIP0） Photosystem II reaction center protein H × 2 （Q8DJ43） Photosystem II reaction center protein I × 2 （Q8DJZ6） Photosystem II reaction center protein J × 2 （P59087） Photosystem II reaction center protein K × 2 （Q9F1K9） Photosystem II reaction center protein L × 2 （Q8DIN8） Photosystem II reaction center protein M × 2 （Q8DHA7） Photosystem II manganese-stabilizing polypeptide × 2 （P0A431） Photosystem II reaction center protein T × 2 （Q8DIQ0） Photosystem II 12 kDa extrinsic protein × 2 （Q9F1L5） Cytochrome c-550 × 2 （P0A386） Photosystem II reaction center protein Ycf12 × 2 （Q8DJI1） Photosystem II reaction center X protein × 2 （Q9F1R6） Photosystem II reaction center protein Z × 2 （Q8DHJ2） Photosystem II protein Y × 2 （Q8DKM3） OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 UNL UNKNOWN LIGAND × 17 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION X-ray crystallization conditions：BATCH MODE；pH 7.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol. Resolution 3.00 Å R-free 0.303

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5KAF Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
11YO Single-conformation model re-refinement of 2F/S3-rich PSII intermediate structure at 2.09 Angstrom resolution Deposited 2026-03-18 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain F 1–45（45 aa）
Chain f 1–45（45 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 38 OEX CA-MN4-O5 CLUSTER × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 PHO PHEOPHYTIN A × 4 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.243
1S5L Architecture of the photosynthetic oxygen evolving center Deposited 2004-01-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain F 1–45（45 aa）
Not recorded FE FE (III) ION × 1 BCT BICARBONATE ION × 2 OEC OXYGEN EVOLVING SYSTEM × 1 CLA CHLOROPHYLL A × 36 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMT DODECYL-BETA-D-MALTOSIDE × 1 BCR BETA-CAROTENE × 7 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pseudo-batch hanging drop；pH 7.5；291 K；100mM HEPES, 100mM (NH4)2SO4, PEG 4000, C12E8, trimethyl lead acetate, pH 7.5, pseudo-batch hanging drop, temperature 291K
Resolution 3.50 Å R-free 0.342
1S5L Architecture of the photosynthetic oxygen evolving center Deposited 2004-01-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain f 1–45（45 aa）
Not recorded FE FE (III) ION × 1 BCT BICARBONATE ION × 2 OEC OXYGEN EVOLVING SYSTEM × 1 CLA CHLOROPHYLL A × 36 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMT DODECYL-BETA-D-MALTOSIDE × 1 BCR BETA-CAROTENE × 7 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pseudo-batch hanging drop；pH 7.5；291 K；100mM HEPES, 100mM (NH4)2SO4, PEG 4000, C12E8, trimethyl lead acetate, pH 7.5, pseudo-batch hanging drop, temperature 291K
Resolution 3.50 Å R-free 0.342
2AXT Crystal Structure of Photosystem II from Thermosynechococcus elongatus Deposited 2005-09-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 36 PDB declaration: 36-meric
Chain F 1–45（45 aa）
Chain f 1–45（45 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PQ9 5-[(2E,6E,10E,14E,18E,22E)-3,7,11,15,19,23,27-HEPTAMETHYLOCTACOSA-2,6,10,14,18,22,26-HEPTAENYL]-2,3-DIMETHYLBENZO-1,4-QUINONE × 4 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 22 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LMT DODECYL-BETA-D-MALTOSIDE × 6 MGE (1S)-2-(ALPHA-L-ALLOPYRANOSYLOXY)-1-[(TRIDECANOYLOXY)METHYL]ETHYL PALMITATE × 12 UNL UNKNOWN LIGAND × 34 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG 2000, PIPES, magnesium chloride, pH 7.0, batch, temperature 298K
Resolution 3.00 Å R-free 0.286
3KZI Crystal Structure of Monomeric Form of Cyanobacterial Photosystem II Deposited 2009-12-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain F 2–45（44 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 11 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 LMT DODECYL-BETA-D-MALTOSIDE × 7 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 BCT BICARBONATE ION × 1 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；291 K；PEG 400, PIPES, MES, CaCl2, pH 7, batch, temperature 291.0K
Resolution 3.60 Å R-free 0.308
4FBY fs X-ray diffraction of Photosystem II Deposited 2012-05-23 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain F 2–45（44 aa）
Chain S 2–45（44 aa）
Not recorded CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 OEC OXYGEN EVOLVING SYSTEM × 2 FE2 FE (II) ION × 2 BCR BETA-CAROTENE × 24 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 CL CHLORIDE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；298 K；4% PEG 2000, 5 mM CaCl2, 100 mM PIPES, pH 7.0, batch, temperature 298K
Resolution 6.56 Å R-free 0.385
4IXQ RT fs X-ray diffraction of Photosystem II, dark state Deposited 2013-01-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain F 1–45（45 aa）
Chain f 1–45（45 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 LMT DODECYL-BETA-D-MALTOSIDE × 14 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.70 Å R-free 0.314
4IXR RT fs X-ray diffraction of Photosystem II, first illuminated state Deposited 2013-01-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain F 1–45（45 aa）
Chain f 1–45（45 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 LMT DODECYL-BETA-D-MALTOSIDE × 14 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.90 Å R-free 0.313
4PBU Serial Time-resolved crystallography of Photosystem II using a femtosecond X-ray laser The S1 state Deposited 2014-04-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain F 12–45（34 aa）
Chain f 12–45（34 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 CA CALCIUM ION × 7 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 5.00 Å R-free 0.262
4PJ0 Structure of T.elongatus Photosystem II, rows of dimers crystal packing Deposited 2014-05-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain F 1–45（45 aa）
Chain f 1–45（45 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE FE (III) ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SO4 SULFATE ION × 10 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 29 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；pH 7.5；293 K；Batch crystallisation: 2 mM Chl a equivalent of PSIIcc mixed 1:1 with: 0.1 M TRIS (pH 7.5), 0.1 M (NH4)2SO4, 15 - 18 % PEG 5000 MME, post-crystallisation dehydration
Resolution 2.44 Å R-free 0.256
4RVY Serial Time resolved crystallography of Photosystem II using a femtosecond X-ray laser. The S state after two flashes (S3) Deposited 2014-11-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain F 12–45（34 aa）
Chain f 12–45（34 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 CA CALCIUM ION × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions Free interface diffusion；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000 , Free interface diffusion, temperature 283K, temperature 283.0K
Resolution 5.50 Å R-free 0.291
4TNH RT XFEL structure of Photosystem II in the dark state at 4.9 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain F 1–45（45 aa）
Chain f 1–45（45 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.90 Å R-free 0.292
4TNI RT XFEL structure of Photosystem II 500 ms after the third illumination at 4.6 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain F 1–45（45 aa）
Chain f 1–45（45 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 2 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.60 Å R-free 0.284
4TNJ RT XFEL structure of Photosystem II 500 ms after the 2nd illumination (2F) at 4.5 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain F 1–45（45 aa）
Chain f 1–45（45 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.50 Å R-free 0.284
4TNK RT XFEL structure of Photosystem II 250 microsec after the third illumination at 5.2 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain F 1–45（45 aa）
Chain f 1–45（45 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.20 Å R-free 0.289
4V62 Crystal Structure of cyanobacterial Photosystem II Deposited 2008-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain AF 1–45（45 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 3 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 13 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 7 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 CL CHLORIDE ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 7 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG2000, PIPES, magnesium chloride, pH7.0, batch, temperature 298K
Resolution 2.90 Å R-free 0.292
4V62 Crystal Structure of cyanobacterial Photosystem II Deposited 2008-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain BF 1–45（45 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 3 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 11 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 7 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 CL CHLORIDE ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 7 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG2000, PIPES, magnesium chloride, pH7.0, batch, temperature 298K
Resolution 2.90 Å R-free 0.292
4V82 Crystal structure of cyanobacterial Photosystem II in complex with terbutryn Deposited 2010-11-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain AF 1–45（45 aa）
Chain BF 1–45（45 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 70 MST 2-T-BUTYLAMINO-4-ETHYLAMINO-6-METHYLTHIO-S-TRIAZINE × 2 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 LMT DODECYL-BETA-D-MALTOSIDE × 16 DMS DIMETHYL SULFOXIDE × 8 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；Pipes, CaCl2, DMSO, PEG 2000, pH 7.0, batch, temperature 298K
Resolution 3.20 Å R-free 0.299
5E79 Macromolecular diffractive imaging using imperfect crystals Deposited 2015-10-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain F 12–45（34 aa）
Chain f 12–45（34 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 CA CALCIUM ION × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；283.18 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 3.50 Å R-free 0.331
5E7C Macromolecular diffractive imaging using imperfect crystals - Bragg data Deposited 2015-10-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain F 12–45（34 aa）
Chain f 12–45（34 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 FE2 FE (II) ION × 2 CA CALCIUM ION × 6 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 4.50 Å R-free 0.275
5H2F Crystal structure of the PsbM-deletion mutant of photosystem II Deposited 2016-10-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain F 13–45（33 aa） Fragment：UNP RESIDUES 13-45
Not recorded OEX CA-MN4-O5 CLUSTER × 1 FE2 FE (II) ION × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 3 LMT DODECYL-BETA-D-MALTOSIDE × 6 UNL UNKNOWN LIGAND × 13 DMS DIMETHYL SULFOXIDE × 67 BCT BICARBONATE ION × 1 CA CALCIUM ION × 2 HTG heptyl 1-thio-beta-D-glucopyranoside × 9 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6.1；285 K；PEG1450, sodium chloride, calcium chloride, magnesium sulfate
Resolution 2.20 Å R-free 0.226
5H2F Crystal structure of the PsbM-deletion mutant of photosystem II Deposited 2016-10-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain f 13–45（33 aa） Fragment：UNP RESIDUES 13-45
Not recorded OEX CA-MN4-O5 CLUSTER × 1 FE2 FE (II) ION × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 5 LMT DODECYL-BETA-D-MALTOSIDE × 5 UNL UNKNOWN LIGAND × 14 DMS DIMETHYL SULFOXIDE × 66 BCT BICARBONATE ION × 1 CA CALCIUM ION × 3 HTG heptyl 1-thio-beta-D-glucopyranoside × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6.1；285 K；PEG1450, sodium chloride, calcium chloride, magnesium sulfate
Resolution 2.20 Å R-free 0.226
5KAI NH3-bound RT XFEL structure of Photosystem II 500 ms after the 2nd illumination (2F) at 2.8 A resolution Deposited 2016-06-01 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain F 1–45（45 aa）
Chain f 1–45（45 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 UNL UNKNOWN LIGAND × 23 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol
Resolution 2.80 Å R-free 0.300
5MX2 Photosystem II depleted of the Mn4CaO5 cluster at 2.55 A resolution Deposited 2017-01-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain F 1–45（45 aa）
Not recorded CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 11 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LFA EICOSANE × 19 FE FE (III) ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 4 BCT BICARBONATE ION × 1 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 7.5；291 K；equal volumes of buffer: 0.1 M Tris-HCl pH 7.5, 0.1 M (NH4)2SO4, 14-18 % PEG 5000 MME and protein: 2 mM chlorophyll equivalent of PSII in C12E8 detergent, post crystallisation treatment
Resolution 2.20 Å R-free 0.260
5MX2 Photosystem II depleted of the Mn4CaO5 cluster at 2.55 A resolution Deposited 2017-01-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain f 1–45（45 aa）
Not recorded CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 11 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LFA EICOSANE × 20 FE FE (III) ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 4 BCT BICARBONATE ION × 1 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 7.5；291 K；equal volumes of buffer: 0.1 M Tris-HCl pH 7.5, 0.1 M (NH4)2SO4, 14-18 % PEG 5000 MME and protein: 2 mM chlorophyll equivalent of PSII in C12E8 detergent, post crystallisation treatment
Resolution 2.20 Å R-free 0.260
5TIS Room temperature XFEL structure of the native, doubly-illuminated photosystem II complex Deposited 2016-10-03 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain F 1–45（45 aa）
Chain f 1–45（45 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 UNL UNKNOWN LIGAND × 33 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol
Resolution 2.25 Å R-free 0.231
5ZZN Crystal structure of photosystem II from an SQDG-deficient mutant of Thermosynechococcus elongatus Deposited 2018-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain F 12–45（34 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 5 UNL UNKNOWN LIGAND × 27 LMT DODECYL-BETA-D-MALTOSIDE × 9 DMS DIMETHYL SULFOXIDE × 48 CA CALCIUM ION × 3 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 HTG heptyl 1-thio-beta-D-glucopyranoside × 12 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 GOL GLYCEROL × 2 FE2 FE (II) ION × 1 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20MM NACL, 10MM CACL2, 40MM MGSO4, 20MM MES BUFFER, PH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPERATURE 285K
Resolution 2.10 Å R-free 0.197
5ZZN Crystal structure of photosystem II from an SQDG-deficient mutant of Thermosynechococcus elongatus Deposited 2018-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain f 12–45（34 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 1 CL CHLORIDE ION × 3 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 5 UNL UNKNOWN LIGAND × 25 LMT DODECYL-BETA-D-MALTOSIDE × 10 DMS DIMETHYL SULFOXIDE × 47 CA CALCIUM ION × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 HTG heptyl 1-thio-beta-D-glucopyranoside × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 GOL GLYCEROL × 3 FE2 FE (II) ION × 1 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20MM NACL, 10MM CACL2, 40MM MGSO4, 20MM MES BUFFER, PH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPERATURE 285K
Resolution 2.10 Å R-free 0.197
6DHE RT XFEL structure of the dark-stable state of Photosystem II (0F, S1-rich) at 2.05 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain F 12–45（34 aa）
Chain f 12–45（34 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 8CT (6'R,11cis,11'cis,13cis,15cis)-4',5'-didehydro-5',6'-dihydro-beta,beta-carotene × 12 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 BCR BETA-CAROTENE × 10 UNL UNKNOWN LIGAND × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.05 Å R-free 0.241
6DHF RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.08 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain F 12–45（34 aa）
Chain f 12–45（34 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 UNL UNKNOWN LIGAND × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.247
6DHG RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.5 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain F 12–45（34 aa）
Chain f 12–45（34 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.50 Å R-free 0.246
6DHH RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.2 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain F 12–45（34 aa）
Chain f 12–45（34 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.20 Å R-free 0.264
6DHO RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.07 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain F 12–45（34 aa）
Chain f 12–45（34 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.07 Å R-free 0.247
6DHP RT XFEL structure of the three-flash state of Photosystem II (3F, S0-rich) at 2.04 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain F 12–45（34 aa）
Chain f 12–45（34 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 UNL UNKNOWN LIGAND × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.04 Å R-free 0.248
6W1O RT XFEL structure of the dark-stable state of Photosystem II (0F, S1-rich) at 2.08 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain F 1–45（45 aa）
Chain f 1–45（45 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.240
6W1P RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.26 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain F 1–45（45 aa）
Chain f 1–45（45 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.26 Å R-free 0.241
6W1Q RT XFEL structure of Photosystem II 50 microseconds after the second illumination at 2.27 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain F 1–45（45 aa）
Chain f 1–45（45 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 BCR BETA-CAROTENE × 22 STE STEARIC ACID × 35 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 HEC HEME C × 4 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.27 Å R-free 0.249
6W1R RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.23 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain F 1–45（45 aa）
Chain f 1–45（45 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.23 Å R-free 0.235
6W1T RT XFEL structure of Photosystem II 250 microseconds after the second illumination at 2.01 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain F 1–45（45 aa）
Chain f 1–45（45 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.01 Å R-free 0.228
6W1U RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.09 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain F 1–45（45 aa）
Chain f 1–45（45 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
6W1V RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain F 1–45（45 aa）
Chain f 1–45（45 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 STE STEARIC ACID × 38 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 PHO PHEOPHYTIN A × 4 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.241
7NHO Structure of PSII-M Deposited 2021-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain F 1–45（45 aa）
Not recorded FE FE (III) ION × 1 MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 BCT BICARBONATE ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.66 Å
7NHP Structure of PSII-I (PSII with Psb27, Psb28, and Psb34) Deposited 2021-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain F 1–45（45 aa）
Not recorded MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 FE FE (III) ION × 1 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.72 Å
7NHQ Structure of PSII-I prime (PSII with Psb28, and Psb34) Deposited 2021-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 17 PDB declaration: heptadecameric
Chain F 1–45（45 aa）
Not recorded FE FE (III) ION × 1 MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.68 Å
7RF1 RT XFEL structure of Photosystem II averaged across all S-states at 1.89 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain F 1–45（45 aa）
Chain f 1–45（45 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 STE STEARIC ACID × 55 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 1.89 Å R-free 0.214
7RF2 RT XFEL structure of dark-stable state of Photosystem II (0F, S1 rich) at 2.08 Angstrom Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain F 1–45（45 aa）
Chain f 1–45（45 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 33 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.238
7RF3 RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.26 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain F 1–45（45 aa）
Chain f 1–45（45 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.26 Å R-free 0.238
7RF4 RT XFEL structure of Photosystem II 50 microseconds after the second illumination at 2.27 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain F 1–45（45 aa）
Chain f 1–45（45 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 BCR BETA-CAROTENE × 22 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.27 Å R-free 0.244
7RF5 RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.23 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain F 1–45（45 aa）
Chain f 1–45（45 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.23 Å R-free 0.233
7RF6 RT XFEL structure of Photosystem II 250 microseconds after the second illumination at 2.01 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain F 1–45（45 aa）
Chain f 1–45（45 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.01 Å R-free 0.227
7RF7 RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.09 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain F 1–45（45 aa）
Chain f 1–45（45 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
7RF8 RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain F 1–45（45 aa）
Chain f 1–45（45 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
7YQ2 Crystal structure of photosystem II expressing psbA2 gene only Deposited 2022-08-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 39 PDB declaration: 39-meric
Chain F 1–45（45 aa）
Chain f 1–45（45 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 UNL UNKNOWN LIGAND × 77 LMT DODECYL-BETA-D-MALTOSIDE × 19 GOL GLYCEROL × 10 BCT BICARBONATE ION × 3 DMS DIMETHYL SULFOXIDE × 116 CA CALCIUM ION × 7 HTG heptyl 1-thio-beta-D-glucopyranoside × 31 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 9 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 MG MAGNESIUM ION × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20mM NaCl, 10mM CaCl2, 40mM MgSO4, 20mM Mes BUFFER, pH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPARATURE 285K
Resolution 1.90 Å R-free 0.184
7YQ7 Crystal structure of photosystem II expressing psbA3 gene only Deposited 2022-08-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 39 PDB declaration: 39-meric
Chain F 1–45（45 aa）
Chain f 1–45（45 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 UNL UNKNOWN LIGAND × 82 GOL GLYCEROL × 11 LMT DODECYL-BETA-D-MALTOSIDE × 18 BCT BICARBONATE ION × 4 DMS DIMETHYL SULFOXIDE × 71 CA CALCIUM ION × 5 HTG heptyl 1-thio-beta-D-glucopyranoside × 18 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 MG MAGNESIUM ION × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20mM NaCl, 10mM CaCl2, 40mM MgSO4, 20mM MES BUFFER pH 6.0, MICRO-BATCH METHOD UNDER OIL, TMPERATURE 285K
Resolution 1.90 Å R-free 0.177
8EZ5 RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2022-10-31 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain F 1–45（45 aa）
Chain f 1–45（45 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.234
8F4C RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain F 1–45（45 aa）
Chain f 1–45（45 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.224
8F4D RT XFEL structure of Photosystem II 50 microseconds after the third illumination at 2.15 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain F 1–45（45 aa）
Chain f 1–45（45 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.15 Å R-free 0.243
8F4E RT XFEL structure of Photosystem II 250 microseconds after the third illumination at 2.09 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain F 1–45（45 aa）
Chain f 1–45（45 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.233
8F4F RT XFEL structure of Photosystem II 500 microseconds after the third illumination at 2.03 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain F 1–45（45 aa）
Chain f 1–45（45 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.03 Å R-free 0.248
8F4G RT XFEL structure of Photosystem II 730 microseconds after the third illumination at 2.03 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain F 1–45（45 aa）
Chain f 1–45（45 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.03 Å R-free 0.223
8F4H RT XFEL structure of Photosystem II 1200 microseconds after the third illumination at 2.10 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain F 1–45（45 aa）
Chain f 1–45（45 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.10 Å R-free 0.232
8F4I RT XFEL structure of Photosystem II 2000 microseconds after the third illumination at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain F 1–45（45 aa）
Chain f 1–45（45 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.232
8F4J RT XFEL structure of Photosystem II 4000 microseconds after the third illumination at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain F 1–45（45 aa）
Chain f 1–45（45 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.226
8F4K RT XFEL structure of the three-flash state of Photosystem II (3F, S0-rich) at 2.16 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain F 1–45（45 aa）
Chain f 1–45（45 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.16 Å R-free 0.241
9EVX cryoEM structure of Photosystem II averaged across S2-S3 states at 1.71 Angstrom resolution Deposited 2024-04-02 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain F 1–45（45 aa）
Chain f 1–45（45 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 STE STEARIC ACID × 42 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 PHO PHEOPHYTIN A × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；100mM MES pH6.5, 5mM CaCl2, 5% glycerol, 0.03% DDM
cryo-EM vitrification conditions Cryogen ETHANE；The sample-on-grid was illuminated with one and two flashes of light
Resolution 1.71 Å
9G6F Inactive PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain F 1–45（45 aa）
Chain f 1–45（45 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LFA EICOSANE × 33 PLM PALMITIC ACID × 24 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 8 LMT DODECYL-BETA-D-MALTOSIDE × 13 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å
9G6G Semi-active PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric
Chain F 1–45（45 aa）
Chain f 1–45（45 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 3 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 LFA EICOSANE × 30 OEX CA-MN4-O5 CLUSTER × 1 LMT DODECYL-BETA-D-MALTOSIDE × 17 BCT BICARBONATE ION × 2 PLM PALMITIC ACID × 29 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 GOL GLYCEROL × 1 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 3 RRX (3R)-beta,beta-caroten-3-ol × 2 CA CALCIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å
9G6H active PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain F 1–45（45 aa）
Chain f 1–45（45 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 PLM PALMITIC ACID × 35 LFA EICOSANE × 24 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 GOL GLYCEROL × 2 LMT DODECYL-BETA-D-MALTOSIDE × 17 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 RRX (3R)-beta,beta-caroten-3-ol × 2 CA CALCIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å

63 other PDB entries and 68 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PSBF_THEEB
Isoform —
PDB entities 6
Chains and sequence ranges Author chain F； PDBConstruct 1–45； UniProt 1–45 Author chain f； PDBConstruct 1–45； UniProt 1–45

Photosystem II reaction center protein H

OrganismNot specified

UniProt Q8DJ43

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 40 PDB declaration: 40-meric（40） Consistent with protein copy count Chain H； UniProt 1–66 Chain h； UniProt 1–66 Not recorded Photosystem II protein D1 1 × 2 （P0A444） Photosystem II CP47 reaction center protein × 2 （Q8DIQ1） Photosystem II CP43 reaction center protein × 2 （Q8DIF8） Photosystem II D2 protein × 2 （Q8CM25） Cytochrome b559 subunit alpha × 2 （Q8DIP0） Cytochrome b559 subunit beta × 2 （Q8DIN9） Photosystem II reaction center protein I × 2 （Q8DJZ6） Photosystem II reaction center protein J × 2 （P59087） Photosystem II reaction center protein K × 2 （Q9F1K9） Photosystem II reaction center protein L × 2 （Q8DIN8） Photosystem II reaction center protein M × 2 （Q8DHA7） Photosystem II manganese-stabilizing polypeptide × 2 （P0A431） Photosystem II reaction center protein T × 2 （Q8DIQ0） Photosystem II 12 kDa extrinsic protein × 2 （Q9F1L5） Cytochrome c-550 × 2 （P0A386） Photosystem II reaction center protein Ycf12 × 2 （Q8DJI1） Photosystem II reaction center X protein × 2 （Q9F1R6） Photosystem II reaction center protein Z × 2 （Q8DHJ2） Photosystem II protein Y × 2 （Q8DKM3） OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 UNL UNKNOWN LIGAND × 17 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION X-ray crystallization conditions：BATCH MODE；pH 7.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol. Resolution 3.00 Å R-free 0.303

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5KAF Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
11YO Single-conformation model re-refinement of 2F/S3-rich PSII intermediate structure at 2.09 Angstrom resolution Deposited 2026-03-18 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain H 1–66（66 aa）
Chain h 1–66（66 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 38 OEX CA-MN4-O5 CLUSTER × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 PHO PHEOPHYTIN A × 4 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.243
1S5L Architecture of the photosynthetic oxygen evolving center Deposited 2004-01-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain H 1–66（66 aa）
Not recorded FE FE (III) ION × 1 BCT BICARBONATE ION × 2 OEC OXYGEN EVOLVING SYSTEM × 1 CLA CHLOROPHYLL A × 36 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMT DODECYL-BETA-D-MALTOSIDE × 1 BCR BETA-CAROTENE × 7 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pseudo-batch hanging drop；pH 7.5；291 K；100mM HEPES, 100mM (NH4)2SO4, PEG 4000, C12E8, trimethyl lead acetate, pH 7.5, pseudo-batch hanging drop, temperature 291K
Resolution 3.50 Å R-free 0.342
1S5L Architecture of the photosynthetic oxygen evolving center Deposited 2004-01-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain h 1–66（66 aa）
Not recorded FE FE (III) ION × 1 BCT BICARBONATE ION × 2 OEC OXYGEN EVOLVING SYSTEM × 1 CLA CHLOROPHYLL A × 36 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMT DODECYL-BETA-D-MALTOSIDE × 1 BCR BETA-CAROTENE × 7 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pseudo-batch hanging drop；pH 7.5；291 K；100mM HEPES, 100mM (NH4)2SO4, PEG 4000, C12E8, trimethyl lead acetate, pH 7.5, pseudo-batch hanging drop, temperature 291K
Resolution 3.50 Å R-free 0.342
2AXT Crystal Structure of Photosystem II from Thermosynechococcus elongatus Deposited 2005-09-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 36 PDB declaration: 36-meric
Chain H 1–66（66 aa）
Chain h 1–66（66 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PQ9 5-[(2E,6E,10E,14E,18E,22E)-3,7,11,15,19,23,27-HEPTAMETHYLOCTACOSA-2,6,10,14,18,22,26-HEPTAENYL]-2,3-DIMETHYLBENZO-1,4-QUINONE × 4 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 22 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LMT DODECYL-BETA-D-MALTOSIDE × 6 MGE (1S)-2-(ALPHA-L-ALLOPYRANOSYLOXY)-1-[(TRIDECANOYLOXY)METHYL]ETHYL PALMITATE × 12 UNL UNKNOWN LIGAND × 34 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG 2000, PIPES, magnesium chloride, pH 7.0, batch, temperature 298K
Resolution 3.00 Å R-free 0.286
3KZI Crystal Structure of Monomeric Form of Cyanobacterial Photosystem II Deposited 2009-12-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain H 2–66（65 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 11 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 LMT DODECYL-BETA-D-MALTOSIDE × 7 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 BCT BICARBONATE ION × 1 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；291 K；PEG 400, PIPES, MES, CaCl2, pH 7, batch, temperature 291.0K
Resolution 3.60 Å R-free 0.308
4FBY fs X-ray diffraction of Photosystem II Deposited 2012-05-23 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain H 2–66（65 aa）
Chain W 2–66（65 aa）
Not recorded CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 OEC OXYGEN EVOLVING SYSTEM × 2 FE2 FE (II) ION × 2 BCR BETA-CAROTENE × 24 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 CL CHLORIDE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；298 K；4% PEG 2000, 5 mM CaCl2, 100 mM PIPES, pH 7.0, batch, temperature 298K
Resolution 6.56 Å R-free 0.385
4IXQ RT fs X-ray diffraction of Photosystem II, dark state Deposited 2013-01-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain H 1–66（66 aa）
Chain h 1–66（66 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 LMT DODECYL-BETA-D-MALTOSIDE × 14 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.70 Å R-free 0.314
4IXR RT fs X-ray diffraction of Photosystem II, first illuminated state Deposited 2013-01-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain H 1–66（66 aa）
Chain h 1–66（66 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 LMT DODECYL-BETA-D-MALTOSIDE × 14 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.90 Å R-free 0.313
4PBU Serial Time-resolved crystallography of Photosystem II using a femtosecond X-ray laser The S1 state Deposited 2014-04-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain H 2–66（65 aa）
Chain h 2–66（65 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 CA CALCIUM ION × 7 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 5.00 Å R-free 0.262
4PJ0 Structure of T.elongatus Photosystem II, rows of dimers crystal packing Deposited 2014-05-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain H 1–66（66 aa）
Chain h 1–66（66 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE FE (III) ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SO4 SULFATE ION × 10 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 29 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；pH 7.5；293 K；Batch crystallisation: 2 mM Chl a equivalent of PSIIcc mixed 1:1 with: 0.1 M TRIS (pH 7.5), 0.1 M (NH4)2SO4, 15 - 18 % PEG 5000 MME, post-crystallisation dehydration
Resolution 2.44 Å R-free 0.256
4RVY Serial Time resolved crystallography of Photosystem II using a femtosecond X-ray laser. The S state after two flashes (S3) Deposited 2014-11-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain H 2–66（65 aa）
Chain h 2–66（65 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 CA CALCIUM ION × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions Free interface diffusion；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000 , Free interface diffusion, temperature 283K, temperature 283.0K
Resolution 5.50 Å R-free 0.291
4TNH RT XFEL structure of Photosystem II in the dark state at 4.9 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain H 1–66（66 aa）
Chain h 1–66（66 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.90 Å R-free 0.292
4TNI RT XFEL structure of Photosystem II 500 ms after the third illumination at 4.6 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain H 1–66（66 aa）
Chain h 1–66（66 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 2 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.60 Å R-free 0.284
4TNJ RT XFEL structure of Photosystem II 500 ms after the 2nd illumination (2F) at 4.5 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain H 1–66（66 aa）
Chain h 1–66（66 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.50 Å R-free 0.284
4TNK RT XFEL structure of Photosystem II 250 microsec after the third illumination at 5.2 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain H 1–66（66 aa）
Chain h 1–66（66 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.20 Å R-free 0.289
4V62 Crystal Structure of cyanobacterial Photosystem II Deposited 2008-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain AH 1–66（66 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 3 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 13 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 7 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 CL CHLORIDE ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 7 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG2000, PIPES, magnesium chloride, pH7.0, batch, temperature 298K
Resolution 2.90 Å R-free 0.292
4V62 Crystal Structure of cyanobacterial Photosystem II Deposited 2008-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain BH 1–66（66 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 3 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 11 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 7 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 CL CHLORIDE ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 7 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG2000, PIPES, magnesium chloride, pH7.0, batch, temperature 298K
Resolution 2.90 Å R-free 0.292
4V82 Crystal structure of cyanobacterial Photosystem II in complex with terbutryn Deposited 2010-11-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain AH 1–66（66 aa）
Chain BH 1–66（66 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 70 MST 2-T-BUTYLAMINO-4-ETHYLAMINO-6-METHYLTHIO-S-TRIAZINE × 2 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 LMT DODECYL-BETA-D-MALTOSIDE × 16 DMS DIMETHYL SULFOXIDE × 8 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；Pipes, CaCl2, DMSO, PEG 2000, pH 7.0, batch, temperature 298K
Resolution 3.20 Å R-free 0.299
5E79 Macromolecular diffractive imaging using imperfect crystals Deposited 2015-10-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain H 2–66（65 aa）
Chain h 2–66（65 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 CA CALCIUM ION × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；283.18 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 3.50 Å R-free 0.331
5E7C Macromolecular diffractive imaging using imperfect crystals - Bragg data Deposited 2015-10-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain H 2–66（65 aa）
Chain h 2–66（65 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 FE2 FE (II) ION × 2 CA CALCIUM ION × 6 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 4.50 Å R-free 0.275
5H2F Crystal structure of the PsbM-deletion mutant of photosystem II Deposited 2016-10-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain H 2–64（63 aa） Fragment：UNP RESIDUES 2-64
Not recorded OEX CA-MN4-O5 CLUSTER × 1 FE2 FE (II) ION × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 3 LMT DODECYL-BETA-D-MALTOSIDE × 6 UNL UNKNOWN LIGAND × 13 DMS DIMETHYL SULFOXIDE × 67 BCT BICARBONATE ION × 1 CA CALCIUM ION × 2 HTG heptyl 1-thio-beta-D-glucopyranoside × 9 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6.1；285 K；PEG1450, sodium chloride, calcium chloride, magnesium sulfate
Resolution 2.20 Å R-free 0.226
5H2F Crystal structure of the PsbM-deletion mutant of photosystem II Deposited 2016-10-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain h 2–64（63 aa） Fragment：UNP RESIDUES 2-64
Not recorded OEX CA-MN4-O5 CLUSTER × 1 FE2 FE (II) ION × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 5 LMT DODECYL-BETA-D-MALTOSIDE × 5 UNL UNKNOWN LIGAND × 14 DMS DIMETHYL SULFOXIDE × 66 BCT BICARBONATE ION × 1 CA CALCIUM ION × 3 HTG heptyl 1-thio-beta-D-glucopyranoside × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6.1；285 K；PEG1450, sodium chloride, calcium chloride, magnesium sulfate
Resolution 2.20 Å R-free 0.226
5KAI NH3-bound RT XFEL structure of Photosystem II 500 ms after the 2nd illumination (2F) at 2.8 A resolution Deposited 2016-06-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain H 2–64（63 aa）
Chain h 2–64（63 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 UNL UNKNOWN LIGAND × 23 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol
Resolution 2.80 Å R-free 0.300
5MX2 Photosystem II depleted of the Mn4CaO5 cluster at 2.55 A resolution Deposited 2017-01-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain H 1–66（66 aa）
Not recorded CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 11 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LFA EICOSANE × 19 FE FE (III) ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 4 BCT BICARBONATE ION × 1 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 7.5；291 K；equal volumes of buffer: 0.1 M Tris-HCl pH 7.5, 0.1 M (NH4)2SO4, 14-18 % PEG 5000 MME and protein: 2 mM chlorophyll equivalent of PSII in C12E8 detergent, post crystallisation treatment
Resolution 2.20 Å R-free 0.260
5MX2 Photosystem II depleted of the Mn4CaO5 cluster at 2.55 A resolution Deposited 2017-01-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain h 1–66（66 aa）
Not recorded CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 11 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LFA EICOSANE × 20 FE FE (III) ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 4 BCT BICARBONATE ION × 1 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 7.5；291 K；equal volumes of buffer: 0.1 M Tris-HCl pH 7.5, 0.1 M (NH4)2SO4, 14-18 % PEG 5000 MME and protein: 2 mM chlorophyll equivalent of PSII in C12E8 detergent, post crystallisation treatment
Resolution 2.20 Å R-free 0.260
5TIS Room temperature XFEL structure of the native, doubly-illuminated photosystem II complex Deposited 2016-10-03 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain H 1–66（66 aa）
Chain h 1–66（66 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 UNL UNKNOWN LIGAND × 33 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol
Resolution 2.25 Å R-free 0.231
5ZZN Crystal structure of photosystem II from an SQDG-deficient mutant of Thermosynechococcus elongatus Deposited 2018-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain H 2–64（63 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 5 UNL UNKNOWN LIGAND × 27 LMT DODECYL-BETA-D-MALTOSIDE × 9 DMS DIMETHYL SULFOXIDE × 48 CA CALCIUM ION × 3 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 HTG heptyl 1-thio-beta-D-glucopyranoside × 12 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 GOL GLYCEROL × 2 FE2 FE (II) ION × 1 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20MM NACL, 10MM CACL2, 40MM MGSO4, 20MM MES BUFFER, PH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPERATURE 285K
Resolution 2.10 Å R-free 0.197
5ZZN Crystal structure of photosystem II from an SQDG-deficient mutant of Thermosynechococcus elongatus Deposited 2018-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain h 2–64（63 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 1 CL CHLORIDE ION × 3 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 5 UNL UNKNOWN LIGAND × 25 LMT DODECYL-BETA-D-MALTOSIDE × 10 DMS DIMETHYL SULFOXIDE × 47 CA CALCIUM ION × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 HTG heptyl 1-thio-beta-D-glucopyranoside × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 GOL GLYCEROL × 3 FE2 FE (II) ION × 1 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20MM NACL, 10MM CACL2, 40MM MGSO4, 20MM MES BUFFER, PH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPERATURE 285K
Resolution 2.10 Å R-free 0.197
6DHE RT XFEL structure of the dark-stable state of Photosystem II (0F, S1-rich) at 2.05 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain H 2–66（65 aa）
Chain h 2–66（65 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 8CT (6'R,11cis,11'cis,13cis,15cis)-4',5'-didehydro-5',6'-dihydro-beta,beta-carotene × 12 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 BCR BETA-CAROTENE × 10 UNL UNKNOWN LIGAND × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.05 Å R-free 0.241
6DHF RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.08 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain H 2–66（65 aa）
Chain h 2–66（65 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 UNL UNKNOWN LIGAND × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.247
6DHG RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.5 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain H 2–66（65 aa）
Chain h 2–66（65 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.50 Å R-free 0.246
6DHH RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.2 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain H 2–66（65 aa）
Chain h 2–66（65 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.20 Å R-free 0.264
6DHO RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.07 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain H 2–66（65 aa）
Chain h 2–66（65 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.07 Å R-free 0.247
6DHP RT XFEL structure of the three-flash state of Photosystem II (3F, S0-rich) at 2.04 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain H 2–66（65 aa）
Chain h 2–66（65 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 UNL UNKNOWN LIGAND × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.04 Å R-free 0.248
6W1O RT XFEL structure of the dark-stable state of Photosystem II (0F, S1-rich) at 2.08 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain H 1–66（66 aa）
Chain h 1–66（66 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.240
6W1P RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.26 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain H 1–66（66 aa）
Chain h 1–66（66 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.26 Å R-free 0.241
6W1Q RT XFEL structure of Photosystem II 50 microseconds after the second illumination at 2.27 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain H 1–66（66 aa）
Chain h 1–66（66 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 BCR BETA-CAROTENE × 22 STE STEARIC ACID × 35 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 HEC HEME C × 4 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.27 Å R-free 0.249
6W1R RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.23 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain H 1–66（66 aa）
Chain h 1–66（66 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.23 Å R-free 0.235
6W1T RT XFEL structure of Photosystem II 250 microseconds after the second illumination at 2.01 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain H 1–66（66 aa）
Chain h 1–66（66 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.01 Å R-free 0.228
6W1U RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.09 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain H 1–66（66 aa）
Chain h 1–66（66 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
6W1V RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain H 1–66（66 aa）
Chain h 1–66（66 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 STE STEARIC ACID × 38 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 PHO PHEOPHYTIN A × 4 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.241
7NHO Structure of PSII-M Deposited 2021-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain H 1–66（66 aa）
Not recorded FE FE (III) ION × 1 MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 BCT BICARBONATE ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.66 Å
7NHP Structure of PSII-I (PSII with Psb27, Psb28, and Psb34) Deposited 2021-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain H 1–66（66 aa）
Not recorded MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 FE FE (III) ION × 1 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.72 Å
7NHQ Structure of PSII-I prime (PSII with Psb28, and Psb34) Deposited 2021-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 17 PDB declaration: heptadecameric
Chain H 1–66（66 aa）
Not recorded FE FE (III) ION × 1 MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.68 Å
7RF1 RT XFEL structure of Photosystem II averaged across all S-states at 1.89 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain H 1–66（66 aa）
Chain h 1–66（66 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 STE STEARIC ACID × 55 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 1.89 Å R-free 0.214
7RF2 RT XFEL structure of dark-stable state of Photosystem II (0F, S1 rich) at 2.08 Angstrom Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain H 1–66（66 aa）
Chain h 1–66（66 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 33 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.238
7RF3 RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.26 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain H 1–66（66 aa）
Chain h 1–66（66 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.26 Å R-free 0.238
7RF4 RT XFEL structure of Photosystem II 50 microseconds after the second illumination at 2.27 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain H 1–66（66 aa）
Chain h 1–66（66 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 BCR BETA-CAROTENE × 22 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.27 Å R-free 0.244
7RF5 RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.23 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain H 1–66（66 aa）
Chain h 1–66（66 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.23 Å R-free 0.233
7RF6 RT XFEL structure of Photosystem II 250 microseconds after the second illumination at 2.01 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain H 1–66（66 aa）
Chain h 1–66（66 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.01 Å R-free 0.227
7RF7 RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.09 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain H 1–66（66 aa）
Chain h 1–66（66 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
7RF8 RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain H 1–66（66 aa）
Chain h 1–66（66 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
7YQ2 Crystal structure of photosystem II expressing psbA2 gene only Deposited 2022-08-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 39 PDB declaration: 39-meric
Chain H 1–66（66 aa）
Chain h 1–66（66 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 UNL UNKNOWN LIGAND × 77 LMT DODECYL-BETA-D-MALTOSIDE × 19 GOL GLYCEROL × 10 BCT BICARBONATE ION × 3 DMS DIMETHYL SULFOXIDE × 116 CA CALCIUM ION × 7 HTG heptyl 1-thio-beta-D-glucopyranoside × 31 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 9 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 MG MAGNESIUM ION × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20mM NaCl, 10mM CaCl2, 40mM MgSO4, 20mM Mes BUFFER, pH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPARATURE 285K
Resolution 1.90 Å R-free 0.184
7YQ7 Crystal structure of photosystem II expressing psbA3 gene only Deposited 2022-08-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 39 PDB declaration: 39-meric
Chain H 1–66（66 aa）
Chain h 1–66（66 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 UNL UNKNOWN LIGAND × 82 GOL GLYCEROL × 11 LMT DODECYL-BETA-D-MALTOSIDE × 18 BCT BICARBONATE ION × 4 DMS DIMETHYL SULFOXIDE × 71 CA CALCIUM ION × 5 HTG heptyl 1-thio-beta-D-glucopyranoside × 18 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 MG MAGNESIUM ION × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20mM NaCl, 10mM CaCl2, 40mM MgSO4, 20mM MES BUFFER pH 6.0, MICRO-BATCH METHOD UNDER OIL, TMPERATURE 285K
Resolution 1.90 Å R-free 0.177
8EZ5 RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2022-10-31 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain H 1–66（66 aa）
Chain h 1–66（66 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.234
8F4C RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain H 1–66（66 aa）
Chain h 1–66（66 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.224
8F4D RT XFEL structure of Photosystem II 50 microseconds after the third illumination at 2.15 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain H 1–66（66 aa）
Chain h 1–66（66 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.15 Å R-free 0.243
8F4E RT XFEL structure of Photosystem II 250 microseconds after the third illumination at 2.09 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain H 1–66（66 aa）
Chain h 1–66（66 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.233
8F4F RT XFEL structure of Photosystem II 500 microseconds after the third illumination at 2.03 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain H 1–66（66 aa）
Chain h 1–66（66 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.03 Å R-free 0.248
8F4G RT XFEL structure of Photosystem II 730 microseconds after the third illumination at 2.03 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain H 1–66（66 aa）
Chain h 1–66（66 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.03 Å R-free 0.223
8F4H RT XFEL structure of Photosystem II 1200 microseconds after the third illumination at 2.10 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain H 1–66（66 aa）
Chain h 1–66（66 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.10 Å R-free 0.232
8F4I RT XFEL structure of Photosystem II 2000 microseconds after the third illumination at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain H 1–66（66 aa）
Chain h 1–66（66 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.232
8F4J RT XFEL structure of Photosystem II 4000 microseconds after the third illumination at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain H 1–66（66 aa）
Chain h 1–66（66 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.226
8F4K RT XFEL structure of the three-flash state of Photosystem II (3F, S0-rich) at 2.16 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain H 1–66（66 aa）
Chain h 1–66（66 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.16 Å R-free 0.241
9EVX cryoEM structure of Photosystem II averaged across S2-S3 states at 1.71 Angstrom resolution Deposited 2024-04-02 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain H 1–66（66 aa）
Chain h 1–66（66 aa）
Mutation：F41C Mutation：F41C FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 STE STEARIC ACID × 42 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 PHO PHEOPHYTIN A × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；100mM MES pH6.5, 5mM CaCl2, 5% glycerol, 0.03% DDM
cryo-EM vitrification conditions Cryogen ETHANE；The sample-on-grid was illuminated with one and two flashes of light
Resolution 1.71 Å
9G6F Inactive PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain H 1–66（66 aa）
Chain h 1–66（66 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LFA EICOSANE × 33 PLM PALMITIC ACID × 24 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 8 LMT DODECYL-BETA-D-MALTOSIDE × 13 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å
9G6G Semi-active PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric
Chain H 1–66（66 aa）
Chain h 1–66（66 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 3 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 LFA EICOSANE × 30 OEX CA-MN4-O5 CLUSTER × 1 LMT DODECYL-BETA-D-MALTOSIDE × 17 BCT BICARBONATE ION × 2 PLM PALMITIC ACID × 29 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 GOL GLYCEROL × 1 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 3 RRX (3R)-beta,beta-caroten-3-ol × 2 CA CALCIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å
9G6H active PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain H 1–66（66 aa）
Chain h 1–66（66 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 PLM PALMITIC ACID × 35 LFA EICOSANE × 24 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 GOL GLYCEROL × 2 LMT DODECYL-BETA-D-MALTOSIDE × 17 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 RRX (3R)-beta,beta-caroten-3-ol × 2 CA CALCIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å

63 other PDB entries and 68 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PSBH_THEEB
Isoform —
PDB entities 7
Chains and sequence ranges Author chain H； PDBConstruct 1–66； UniProt 1–66 Author chain h； PDBConstruct 1–66； UniProt 1–66

Photosystem II reaction center protein I

OrganismNot specified

UniProt Q8DJZ6

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 40 PDB declaration: 40-meric（40） Consistent with protein copy count Chain I； UniProt 2–38 Chain i； UniProt 2–38 Non-standard monomer：Yes (specific site not provided by mmCIF) Photosystem II protein D1 1 × 2 （P0A444） Photosystem II CP47 reaction center protein × 2 （Q8DIQ1） Photosystem II CP43 reaction center protein × 2 （Q8DIF8） Photosystem II D2 protein × 2 （Q8CM25） Cytochrome b559 subunit alpha × 2 （Q8DIP0） Cytochrome b559 subunit beta × 2 （Q8DIN9） Photosystem II reaction center protein H × 2 （Q8DJ43） Photosystem II reaction center protein J × 2 （P59087） Photosystem II reaction center protein K × 2 （Q9F1K9） Photosystem II reaction center protein L × 2 （Q8DIN8） Photosystem II reaction center protein M × 2 （Q8DHA7） Photosystem II manganese-stabilizing polypeptide × 2 （P0A431） Photosystem II reaction center protein T × 2 （Q8DIQ0） Photosystem II 12 kDa extrinsic protein × 2 （Q9F1L5） Cytochrome c-550 × 2 （P0A386） Photosystem II reaction center protein Ycf12 × 2 （Q8DJI1） Photosystem II reaction center X protein × 2 （Q9F1R6） Photosystem II reaction center protein Z × 2 （Q8DHJ2） Photosystem II protein Y × 2 （Q8DKM3） OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 UNL UNKNOWN LIGAND × 17 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION X-ray crystallization conditions：BATCH MODE；pH 7.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol. Resolution 3.00 Å R-free 0.303

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5KAF Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
11YO Single-conformation model re-refinement of 2F/S3-rich PSII intermediate structure at 2.09 Angstrom resolution Deposited 2026-03-18 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain I 1–38（38 aa）
Chain i 1–38（38 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 38 OEX CA-MN4-O5 CLUSTER × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 PHO PHEOPHYTIN A × 4 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.243
1S5L Architecture of the photosynthetic oxygen evolving center Deposited 2004-01-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain I 1–38（38 aa）
Not recorded FE FE (III) ION × 1 BCT BICARBONATE ION × 2 OEC OXYGEN EVOLVING SYSTEM × 1 CLA CHLOROPHYLL A × 36 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMT DODECYL-BETA-D-MALTOSIDE × 1 BCR BETA-CAROTENE × 7 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pseudo-batch hanging drop；pH 7.5；291 K；100mM HEPES, 100mM (NH4)2SO4, PEG 4000, C12E8, trimethyl lead acetate, pH 7.5, pseudo-batch hanging drop, temperature 291K
Resolution 3.50 Å R-free 0.342
1S5L Architecture of the photosynthetic oxygen evolving center Deposited 2004-01-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain i 1–38（38 aa）
Not recorded FE FE (III) ION × 1 BCT BICARBONATE ION × 2 OEC OXYGEN EVOLVING SYSTEM × 1 CLA CHLOROPHYLL A × 36 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMT DODECYL-BETA-D-MALTOSIDE × 1 BCR BETA-CAROTENE × 7 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pseudo-batch hanging drop；pH 7.5；291 K；100mM HEPES, 100mM (NH4)2SO4, PEG 4000, C12E8, trimethyl lead acetate, pH 7.5, pseudo-batch hanging drop, temperature 291K
Resolution 3.50 Å R-free 0.342
2AXT Crystal Structure of Photosystem II from Thermosynechococcus elongatus Deposited 2005-09-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 36 PDB declaration: 36-meric
Chain I 1–38（38 aa）
Chain i 1–38（38 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PQ9 5-[(2E,6E,10E,14E,18E,22E)-3,7,11,15,19,23,27-HEPTAMETHYLOCTACOSA-2,6,10,14,18,22,26-HEPTAENYL]-2,3-DIMETHYLBENZO-1,4-QUINONE × 4 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 22 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LMT DODECYL-BETA-D-MALTOSIDE × 6 MGE (1S)-2-(ALPHA-L-ALLOPYRANOSYLOXY)-1-[(TRIDECANOYLOXY)METHYL]ETHYL PALMITATE × 12 UNL UNKNOWN LIGAND × 34 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG 2000, PIPES, magnesium chloride, pH 7.0, batch, temperature 298K
Resolution 3.00 Å R-free 0.286
3KZI Crystal Structure of Monomeric Form of Cyanobacterial Photosystem II Deposited 2009-12-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain I 1–38（38 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 11 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 LMT DODECYL-BETA-D-MALTOSIDE × 7 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 BCT BICARBONATE ION × 1 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；291 K；PEG 400, PIPES, MES, CaCl2, pH 7, batch, temperature 291.0K
Resolution 3.60 Å R-free 0.308
4FBY fs X-ray diffraction of Photosystem II Deposited 2012-05-23 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain I 1–38（38 aa）
Chain a 1–38（38 aa）
Not recorded CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 OEC OXYGEN EVOLVING SYSTEM × 2 FE2 FE (II) ION × 2 BCR BETA-CAROTENE × 24 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 CL CHLORIDE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；298 K；4% PEG 2000, 5 mM CaCl2, 100 mM PIPES, pH 7.0, batch, temperature 298K
Resolution 6.56 Å R-free 0.385
4IXQ RT fs X-ray diffraction of Photosystem II, dark state Deposited 2013-01-27 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain I 1–38（38 aa）
Chain i 1–38（38 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 LMT DODECYL-BETA-D-MALTOSIDE × 14 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.70 Å R-free 0.314
4IXR RT fs X-ray diffraction of Photosystem II, first illuminated state Deposited 2013-01-27 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain I 1–38（38 aa）
Chain i 1–38（38 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 LMT DODECYL-BETA-D-MALTOSIDE × 14 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.90 Å R-free 0.313
4PBU Serial Time-resolved crystallography of Photosystem II using a femtosecond X-ray laser The S1 state Deposited 2014-04-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain I 1–38（38 aa）
Chain i 1–38（38 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 CA CALCIUM ION × 7 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 5.00 Å R-free 0.262
4PJ0 Structure of T.elongatus Photosystem II, rows of dimers crystal packing Deposited 2014-05-10 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain I 1–38（38 aa）
Chain i 1–38（38 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE FE (III) ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SO4 SULFATE ION × 10 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 29 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；pH 7.5；293 K；Batch crystallisation: 2 mM Chl a equivalent of PSIIcc mixed 1:1 with: 0.1 M TRIS (pH 7.5), 0.1 M (NH4)2SO4, 15 - 18 % PEG 5000 MME, post-crystallisation dehydration
Resolution 2.44 Å R-free 0.256
4RVY Serial Time resolved crystallography of Photosystem II using a femtosecond X-ray laser. The S state after two flashes (S3) Deposited 2014-11-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain I 1–38（38 aa）
Chain i 1–38（38 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 CA CALCIUM ION × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions Free interface diffusion；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000 , Free interface diffusion, temperature 283K, temperature 283.0K
Resolution 5.50 Å R-free 0.291
4TNH RT XFEL structure of Photosystem II in the dark state at 4.9 A resolution Deposited 2014-06-04 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain I 1–38（38 aa）
Chain i 1–38（38 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.90 Å R-free 0.292
4TNI RT XFEL structure of Photosystem II 500 ms after the third illumination at 4.6 A resolution Deposited 2014-06-04 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain I 1–38（38 aa）
Chain i 1–38（38 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 2 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.60 Å R-free 0.284
4TNJ RT XFEL structure of Photosystem II 500 ms after the 2nd illumination (2F) at 4.5 A resolution Deposited 2014-06-04 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain I 1–38（38 aa）
Chain i 1–38（38 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.50 Å R-free 0.284
4TNK RT XFEL structure of Photosystem II 250 microsec after the third illumination at 5.2 A resolution Deposited 2014-06-04 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain I 1–38（38 aa）
Chain i 1–38（38 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.20 Å R-free 0.289
4V62 Crystal Structure of cyanobacterial Photosystem II Deposited 2008-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain AI 1–38（38 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 3 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 13 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 7 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 CL CHLORIDE ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 7 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG2000, PIPES, magnesium chloride, pH7.0, batch, temperature 298K
Resolution 2.90 Å R-free 0.292
4V62 Crystal Structure of cyanobacterial Photosystem II Deposited 2008-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain BI 1–38（38 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 3 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 11 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 7 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 CL CHLORIDE ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 7 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG2000, PIPES, magnesium chloride, pH7.0, batch, temperature 298K
Resolution 2.90 Å R-free 0.292
4V82 Crystal structure of cyanobacterial Photosystem II in complex with terbutryn Deposited 2010-11-30 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain AI 1–38（38 aa）
Chain BI 1–38（38 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 70 MST 2-T-BUTYLAMINO-4-ETHYLAMINO-6-METHYLTHIO-S-TRIAZINE × 2 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 LMT DODECYL-BETA-D-MALTOSIDE × 16 DMS DIMETHYL SULFOXIDE × 8 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；Pipes, CaCl2, DMSO, PEG 2000, pH 7.0, batch, temperature 298K
Resolution 3.20 Å R-free 0.299
5E79 Macromolecular diffractive imaging using imperfect crystals Deposited 2015-10-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain I 1–38（38 aa）
Chain i 1–38（38 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 CA CALCIUM ION × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；283.18 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 3.50 Å R-free 0.331
5E7C Macromolecular diffractive imaging using imperfect crystals - Bragg data Deposited 2015-10-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain I 1–38（38 aa）
Chain i 1–38（38 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 FE2 FE (II) ION × 2 CA CALCIUM ION × 6 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 4.50 Å R-free 0.275
5H2F Crystal structure of the PsbM-deletion mutant of photosystem II Deposited 2016-10-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain I 1–36（36 aa） Fragment：UNP RESIDUES 1-36
Non-standard monomer：Yes (specific site not provided by mmCIF) OEX CA-MN4-O5 CLUSTER × 1 FE2 FE (II) ION × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 3 LMT DODECYL-BETA-D-MALTOSIDE × 6 UNL UNKNOWN LIGAND × 13 DMS DIMETHYL SULFOXIDE × 67 BCT BICARBONATE ION × 1 CA CALCIUM ION × 2 HTG heptyl 1-thio-beta-D-glucopyranoside × 9 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6.1；285 K；PEG1450, sodium chloride, calcium chloride, magnesium sulfate
Resolution 2.20 Å R-free 0.226
5H2F Crystal structure of the PsbM-deletion mutant of photosystem II Deposited 2016-10-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain i 1–36（36 aa） Fragment：UNP RESIDUES 1-36
Non-standard monomer：Yes (specific site not provided by mmCIF) OEX CA-MN4-O5 CLUSTER × 1 FE2 FE (II) ION × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 5 LMT DODECYL-BETA-D-MALTOSIDE × 5 UNL UNKNOWN LIGAND × 14 DMS DIMETHYL SULFOXIDE × 66 BCT BICARBONATE ION × 1 CA CALCIUM ION × 3 HTG heptyl 1-thio-beta-D-glucopyranoside × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6.1；285 K；PEG1450, sodium chloride, calcium chloride, magnesium sulfate
Resolution 2.20 Å R-free 0.226
5KAI NH3-bound RT XFEL structure of Photosystem II 500 ms after the 2nd illumination (2F) at 2.8 A resolution Deposited 2016-06-01 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain I 2–38（37 aa）
Chain i 2–38（37 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 UNL UNKNOWN LIGAND × 23 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol
Resolution 2.80 Å R-free 0.300
5MX2 Photosystem II depleted of the Mn4CaO5 cluster at 2.55 A resolution Deposited 2017-01-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain I 1–38（38 aa）
Not recorded CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 11 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LFA EICOSANE × 19 FE FE (III) ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 4 BCT BICARBONATE ION × 1 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 7.5；291 K；equal volumes of buffer: 0.1 M Tris-HCl pH 7.5, 0.1 M (NH4)2SO4, 14-18 % PEG 5000 MME and protein: 2 mM chlorophyll equivalent of PSII in C12E8 detergent, post crystallisation treatment
Resolution 2.20 Å R-free 0.260
5MX2 Photosystem II depleted of the Mn4CaO5 cluster at 2.55 A resolution Deposited 2017-01-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain i 1–38（38 aa）
Not recorded CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 11 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LFA EICOSANE × 20 FE FE (III) ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 4 BCT BICARBONATE ION × 1 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 7.5；291 K；equal volumes of buffer: 0.1 M Tris-HCl pH 7.5, 0.1 M (NH4)2SO4, 14-18 % PEG 5000 MME and protein: 2 mM chlorophyll equivalent of PSII in C12E8 detergent, post crystallisation treatment
Resolution 2.20 Å R-free 0.260
5TIS Room temperature XFEL structure of the native, doubly-illuminated photosystem II complex Deposited 2016-10-03 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain I 1–38（38 aa）
Chain i 1–38（38 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 UNL UNKNOWN LIGAND × 33 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol
Resolution 2.25 Å R-free 0.231
5ZZN Crystal structure of photosystem II from an SQDG-deficient mutant of Thermosynechococcus elongatus Deposited 2018-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain I 1–37（37 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) OEX CA-MN4-O5 CLUSTER × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 5 UNL UNKNOWN LIGAND × 27 LMT DODECYL-BETA-D-MALTOSIDE × 9 DMS DIMETHYL SULFOXIDE × 48 CA CALCIUM ION × 3 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 HTG heptyl 1-thio-beta-D-glucopyranoside × 12 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 GOL GLYCEROL × 2 FE2 FE (II) ION × 1 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20MM NACL, 10MM CACL2, 40MM MGSO4, 20MM MES BUFFER, PH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPERATURE 285K
Resolution 2.10 Å R-free 0.197
5ZZN Crystal structure of photosystem II from an SQDG-deficient mutant of Thermosynechococcus elongatus Deposited 2018-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain i 1–37（37 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) OEX CA-MN4-O5 CLUSTER × 1 CL CHLORIDE ION × 3 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 5 UNL UNKNOWN LIGAND × 25 LMT DODECYL-BETA-D-MALTOSIDE × 10 DMS DIMETHYL SULFOXIDE × 47 CA CALCIUM ION × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 HTG heptyl 1-thio-beta-D-glucopyranoside × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 GOL GLYCEROL × 3 FE2 FE (II) ION × 1 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20MM NACL, 10MM CACL2, 40MM MGSO4, 20MM MES BUFFER, PH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPERATURE 285K
Resolution 2.10 Å R-free 0.197
6DHE RT XFEL structure of the dark-stable state of Photosystem II (0F, S1-rich) at 2.05 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain I 1–36（36 aa）
Chain i 1–36（36 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 8CT (6'R,11cis,11'cis,13cis,15cis)-4',5'-didehydro-5',6'-dihydro-beta,beta-carotene × 12 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 BCR BETA-CAROTENE × 10 UNL UNKNOWN LIGAND × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.05 Å R-free 0.241
6DHF RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.08 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain I 1–36（36 aa）
Chain i 1–36（36 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 UNL UNKNOWN LIGAND × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.247
6DHG RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.5 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain I 1–36（36 aa）
Chain i 1–36（36 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.50 Å R-free 0.246
6DHH RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.2 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain I 1–36（36 aa）
Chain i 1–36（36 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.20 Å R-free 0.264
6DHO RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.07 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain I 1–36（36 aa）
Chain i 1–36（36 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.07 Å R-free 0.247
6DHP RT XFEL structure of the three-flash state of Photosystem II (3F, S0-rich) at 2.04 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain I 1–36（36 aa）
Chain i 1–36（36 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 UNL UNKNOWN LIGAND × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.04 Å R-free 0.248
6W1O RT XFEL structure of the dark-stable state of Photosystem II (0F, S1-rich) at 2.08 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain I 2–38（37 aa）
Chain i 2–38（37 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.240
6W1P RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.26 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain I 2–38（37 aa）
Chain i 2–38（37 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.26 Å R-free 0.241
6W1Q RT XFEL structure of Photosystem II 50 microseconds after the second illumination at 2.27 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain I 2–38（37 aa）
Chain i 2–38（37 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 BCR BETA-CAROTENE × 22 STE STEARIC ACID × 35 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 HEC HEME C × 4 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.27 Å R-free 0.249
6W1R RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.23 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain I 2–38（37 aa）
Chain i 2–38（37 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.23 Å R-free 0.235
6W1T RT XFEL structure of Photosystem II 250 microseconds after the second illumination at 2.01 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain I 2–38（37 aa）
Chain i 2–38（37 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.01 Å R-free 0.228
6W1U RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.09 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain I 2–38（37 aa）
Chain i 2–38（37 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
6W1V RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain I 2–38（37 aa）
Chain i 2–38（37 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 STE STEARIC ACID × 38 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 PHO PHEOPHYTIN A × 4 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.241
7NHO Structure of PSII-M Deposited 2021-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain I 1–38（38 aa）
Not recorded FE FE (III) ION × 1 MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 BCT BICARBONATE ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.66 Å
7NHP Structure of PSII-I (PSII with Psb27, Psb28, and Psb34) Deposited 2021-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain I 1–38（38 aa）
Not recorded MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 FE FE (III) ION × 1 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.72 Å
7NHQ Structure of PSII-I prime (PSII with Psb28, and Psb34) Deposited 2021-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 17 PDB declaration: heptadecameric
Chain I 1–38（38 aa）
Not recorded FE FE (III) ION × 1 MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.68 Å
7RF1 RT XFEL structure of Photosystem II averaged across all S-states at 1.89 Angstrom resolution Deposited 2021-07-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain I 1–38（38 aa）
Chain i 1–38（38 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 STE STEARIC ACID × 55 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 1.89 Å R-free 0.214
7RF2 RT XFEL structure of dark-stable state of Photosystem II (0F, S1 rich) at 2.08 Angstrom Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain I 2–38（37 aa）
Chain i 2–38（37 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 33 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.238
7RF3 RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.26 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain I 2–38（37 aa）
Chain i 2–38（37 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.26 Å R-free 0.238
7RF4 RT XFEL structure of Photosystem II 50 microseconds after the second illumination at 2.27 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain I 2–38（37 aa）
Chain i 2–38（37 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 BCR BETA-CAROTENE × 22 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.27 Å R-free 0.244
7RF5 RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.23 Angstrom resolution Deposited 2021-07-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain I 1–38（38 aa）
Chain i 1–38（38 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.23 Å R-free 0.233
7RF6 RT XFEL structure of Photosystem II 250 microseconds after the second illumination at 2.01 Angstrom resolution Deposited 2021-07-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain I 1–38（38 aa）
Chain i 1–38（38 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.01 Å R-free 0.227
7RF7 RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.09 Angstrom resolution Deposited 2021-07-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain I 1–38（38 aa）
Chain i 1–38（38 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
7RF8 RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2021-07-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain I 1–38（38 aa）
Chain i 1–38（38 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
7YQ2 Crystal structure of photosystem II expressing psbA2 gene only Deposited 2022-08-05 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 39 PDB declaration: 39-meric
Chain I 1–38（38 aa）
Chain i 1–38（38 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 UNL UNKNOWN LIGAND × 77 LMT DODECYL-BETA-D-MALTOSIDE × 19 GOL GLYCEROL × 10 BCT BICARBONATE ION × 3 DMS DIMETHYL SULFOXIDE × 116 CA CALCIUM ION × 7 HTG heptyl 1-thio-beta-D-glucopyranoside × 31 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 9 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 MG MAGNESIUM ION × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20mM NaCl, 10mM CaCl2, 40mM MgSO4, 20mM Mes BUFFER, pH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPARATURE 285K
Resolution 1.90 Å R-free 0.184
7YQ7 Crystal structure of photosystem II expressing psbA3 gene only Deposited 2022-08-05 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 39 PDB declaration: 39-meric
Chain I 1–38（38 aa）
Chain i 1–38（38 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 UNL UNKNOWN LIGAND × 82 GOL GLYCEROL × 11 LMT DODECYL-BETA-D-MALTOSIDE × 18 BCT BICARBONATE ION × 4 DMS DIMETHYL SULFOXIDE × 71 CA CALCIUM ION × 5 HTG heptyl 1-thio-beta-D-glucopyranoside × 18 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 MG MAGNESIUM ION × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20mM NaCl, 10mM CaCl2, 40mM MgSO4, 20mM MES BUFFER pH 6.0, MICRO-BATCH METHOD UNDER OIL, TMPERATURE 285K
Resolution 1.90 Å R-free 0.177
8EZ5 RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2022-10-31 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain I 1–38（38 aa）
Chain i 1–38（38 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.234
8F4C RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain I 2–38（37 aa）
Chain i 2–38（37 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.224
8F4D RT XFEL structure of Photosystem II 50 microseconds after the third illumination at 2.15 Angstrom resolution Deposited 2022-11-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain I 1–38（38 aa）
Chain i 1–38（38 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.15 Å R-free 0.243
8F4E RT XFEL structure of Photosystem II 250 microseconds after the third illumination at 2.09 Angstrom resolution Deposited 2022-11-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain I 1–38（38 aa）
Chain i 1–38（38 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.233
8F4F RT XFEL structure of Photosystem II 500 microseconds after the third illumination at 2.03 Angstrom resolution Deposited 2022-11-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain I 1–38（38 aa）
Chain i 1–38（38 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.03 Å R-free 0.248
8F4G RT XFEL structure of Photosystem II 730 microseconds after the third illumination at 2.03 Angstrom resolution Deposited 2022-11-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain I 1–38（38 aa）
Chain i 1–38（38 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.03 Å R-free 0.223
8F4H RT XFEL structure of Photosystem II 1200 microseconds after the third illumination at 2.10 Angstrom resolution Deposited 2022-11-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain I 1–38（38 aa）
Chain i 1–38（38 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.10 Å R-free 0.232
8F4I RT XFEL structure of Photosystem II 2000 microseconds after the third illumination at 2.00 Angstrom resolution Deposited 2022-11-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain I 1–38（38 aa）
Chain i 1–38（38 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.232
8F4J RT XFEL structure of Photosystem II 4000 microseconds after the third illumination at 2.00 Angstrom resolution Deposited 2022-11-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain I 1–38（38 aa）
Chain i 1–38（38 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.226
8F4K RT XFEL structure of the three-flash state of Photosystem II (3F, S0-rich) at 2.16 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain I 2–38（37 aa）
Chain i 2–38（37 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.16 Å R-free 0.241
9EVX cryoEM structure of Photosystem II averaged across S2-S3 states at 1.71 Angstrom resolution Deposited 2024-04-02 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain I 1–38（38 aa）
Chain i 1–38（38 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 STE STEARIC ACID × 42 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 PHO PHEOPHYTIN A × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；100mM MES pH6.5, 5mM CaCl2, 5% glycerol, 0.03% DDM
cryo-EM vitrification conditions Cryogen ETHANE；The sample-on-grid was illuminated with one and two flashes of light
Resolution 1.71 Å
9G6F Inactive PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain I 1–38（38 aa）
Chain i 1–38（38 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LFA EICOSANE × 33 PLM PALMITIC ACID × 24 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 8 LMT DODECYL-BETA-D-MALTOSIDE × 13 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å
9G6G Semi-active PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric
Chain I 1–38（38 aa）
Chain i 1–38（38 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CL CHLORIDE ION × 3 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 LFA EICOSANE × 30 OEX CA-MN4-O5 CLUSTER × 1 LMT DODECYL-BETA-D-MALTOSIDE × 17 BCT BICARBONATE ION × 2 PLM PALMITIC ACID × 29 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 GOL GLYCEROL × 1 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 3 RRX (3R)-beta,beta-caroten-3-ol × 2 CA CALCIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å
9G6H active PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain I 1–38（38 aa）
Chain i 1–38（38 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 PLM PALMITIC ACID × 35 LFA EICOSANE × 24 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 GOL GLYCEROL × 2 LMT DODECYL-BETA-D-MALTOSIDE × 17 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 RRX (3R)-beta,beta-caroten-3-ol × 2 CA CALCIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å

63 other PDB entries and 68 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PSBI_THEEB
Isoform —
PDB entities 8
Chains and sequence ranges Author chain I； PDBConstruct 2–38； UniProt 2–38 Author chain i； PDBConstruct 2–38； UniProt 2–38

Photosystem II reaction center protein J

OrganismNot specified

UniProt P59087

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 40 PDB declaration: 40-meric（40） Consistent with protein copy count Chain J； UniProt 1–40 Chain j； UniProt 1–40 Not recorded Photosystem II protein D1 1 × 2 （P0A444） Photosystem II CP47 reaction center protein × 2 （Q8DIQ1） Photosystem II CP43 reaction center protein × 2 （Q8DIF8） Photosystem II D2 protein × 2 （Q8CM25） Cytochrome b559 subunit alpha × 2 （Q8DIP0） Cytochrome b559 subunit beta × 2 （Q8DIN9） Photosystem II reaction center protein H × 2 （Q8DJ43） Photosystem II reaction center protein I × 2 （Q8DJZ6） Photosystem II reaction center protein K × 2 （Q9F1K9） Photosystem II reaction center protein L × 2 （Q8DIN8） Photosystem II reaction center protein M × 2 （Q8DHA7） Photosystem II manganese-stabilizing polypeptide × 2 （P0A431） Photosystem II reaction center protein T × 2 （Q8DIQ0） Photosystem II 12 kDa extrinsic protein × 2 （Q9F1L5） Cytochrome c-550 × 2 （P0A386） Photosystem II reaction center protein Ycf12 × 2 （Q8DJI1） Photosystem II reaction center X protein × 2 （Q9F1R6） Photosystem II reaction center protein Z × 2 （Q8DHJ2） Photosystem II protein Y × 2 （Q8DKM3） OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 UNL UNKNOWN LIGAND × 17 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION X-ray crystallization conditions：BATCH MODE；pH 7.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol. Resolution 3.00 Å R-free 0.303

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5KAF Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
11YO Single-conformation model re-refinement of 2F/S3-rich PSII intermediate structure at 2.09 Angstrom resolution Deposited 2026-03-18 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain J 1–40（40 aa）
Chain j 1–40（40 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 38 OEX CA-MN4-O5 CLUSTER × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 PHO PHEOPHYTIN A × 4 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.243
1S5L Architecture of the photosynthetic oxygen evolving center Deposited 2004-01-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain J 1–40（40 aa）
Not recorded FE FE (III) ION × 1 BCT BICARBONATE ION × 2 OEC OXYGEN EVOLVING SYSTEM × 1 CLA CHLOROPHYLL A × 36 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMT DODECYL-BETA-D-MALTOSIDE × 1 BCR BETA-CAROTENE × 7 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pseudo-batch hanging drop；pH 7.5；291 K；100mM HEPES, 100mM (NH4)2SO4, PEG 4000, C12E8, trimethyl lead acetate, pH 7.5, pseudo-batch hanging drop, temperature 291K
Resolution 3.50 Å R-free 0.342
1S5L Architecture of the photosynthetic oxygen evolving center Deposited 2004-01-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain j 1–40（40 aa）
Not recorded FE FE (III) ION × 1 BCT BICARBONATE ION × 2 OEC OXYGEN EVOLVING SYSTEM × 1 CLA CHLOROPHYLL A × 36 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMT DODECYL-BETA-D-MALTOSIDE × 1 BCR BETA-CAROTENE × 7 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pseudo-batch hanging drop；pH 7.5；291 K；100mM HEPES, 100mM (NH4)2SO4, PEG 4000, C12E8, trimethyl lead acetate, pH 7.5, pseudo-batch hanging drop, temperature 291K
Resolution 3.50 Å R-free 0.342
2AXT Crystal Structure of Photosystem II from Thermosynechococcus elongatus Deposited 2005-09-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 36 PDB declaration: 36-meric
Chain J 1–40（40 aa）
Chain j 1–40（40 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PQ9 5-[(2E,6E,10E,14E,18E,22E)-3,7,11,15,19,23,27-HEPTAMETHYLOCTACOSA-2,6,10,14,18,22,26-HEPTAENYL]-2,3-DIMETHYLBENZO-1,4-QUINONE × 4 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 22 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LMT DODECYL-BETA-D-MALTOSIDE × 6 MGE (1S)-2-(ALPHA-L-ALLOPYRANOSYLOXY)-1-[(TRIDECANOYLOXY)METHYL]ETHYL PALMITATE × 12 UNL UNKNOWN LIGAND × 34 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG 2000, PIPES, magnesium chloride, pH 7.0, batch, temperature 298K
Resolution 3.00 Å R-free 0.286
3KZI Crystal Structure of Monomeric Form of Cyanobacterial Photosystem II Deposited 2009-12-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain J 1–40（40 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 11 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 LMT DODECYL-BETA-D-MALTOSIDE × 7 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 BCT BICARBONATE ION × 1 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；291 K；PEG 400, PIPES, MES, CaCl2, pH 7, batch, temperature 291.0K
Resolution 3.60 Å R-free 0.308
4FBY fs X-ray diffraction of Photosystem II Deposited 2012-05-23 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain J 2–40（39 aa）
Chain b 2–40（39 aa）
Not recorded CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 OEC OXYGEN EVOLVING SYSTEM × 2 FE2 FE (II) ION × 2 BCR BETA-CAROTENE × 24 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 CL CHLORIDE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；298 K；4% PEG 2000, 5 mM CaCl2, 100 mM PIPES, pH 7.0, batch, temperature 298K
Resolution 6.56 Å R-free 0.385
4IXQ RT fs X-ray diffraction of Photosystem II, dark state Deposited 2013-01-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain J 1–40（40 aa）
Chain j 1–40（40 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 LMT DODECYL-BETA-D-MALTOSIDE × 14 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.70 Å R-free 0.314
4IXR RT fs X-ray diffraction of Photosystem II, first illuminated state Deposited 2013-01-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain J 1–40（40 aa）
Chain j 1–40（40 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 LMT DODECYL-BETA-D-MALTOSIDE × 14 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.90 Å R-free 0.313
4PBU Serial Time-resolved crystallography of Photosystem II using a femtosecond X-ray laser The S1 state Deposited 2014-04-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain J 1–40（40 aa）
Chain j 1–40（40 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 CA CALCIUM ION × 7 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 5.00 Å R-free 0.262
4PJ0 Structure of T.elongatus Photosystem II, rows of dimers crystal packing Deposited 2014-05-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain J 1–40（40 aa）
Chain j 1–40（40 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE FE (III) ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SO4 SULFATE ION × 10 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 29 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；pH 7.5；293 K；Batch crystallisation: 2 mM Chl a equivalent of PSIIcc mixed 1:1 with: 0.1 M TRIS (pH 7.5), 0.1 M (NH4)2SO4, 15 - 18 % PEG 5000 MME, post-crystallisation dehydration
Resolution 2.44 Å R-free 0.256
4RVY Serial Time resolved crystallography of Photosystem II using a femtosecond X-ray laser. The S state after two flashes (S3) Deposited 2014-11-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain J 1–40（40 aa）
Chain j 1–40（40 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 CA CALCIUM ION × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions Free interface diffusion；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000 , Free interface diffusion, temperature 283K, temperature 283.0K
Resolution 5.50 Å R-free 0.291
4TNH RT XFEL structure of Photosystem II in the dark state at 4.9 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain J 1–40（40 aa）
Chain j 1–40（40 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.90 Å R-free 0.292
4TNI RT XFEL structure of Photosystem II 500 ms after the third illumination at 4.6 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain J 1–40（40 aa）
Chain j 1–40（40 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 2 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.60 Å R-free 0.284
4TNJ RT XFEL structure of Photosystem II 500 ms after the 2nd illumination (2F) at 4.5 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain J 1–40（40 aa）
Chain j 1–40（40 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.50 Å R-free 0.284
4TNK RT XFEL structure of Photosystem II 250 microsec after the third illumination at 5.2 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain J 1–40（40 aa）
Chain j 1–40（40 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.20 Å R-free 0.289
4V62 Crystal Structure of cyanobacterial Photosystem II Deposited 2008-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain AJ 1–40（40 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 3 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 13 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 7 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 CL CHLORIDE ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 7 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG2000, PIPES, magnesium chloride, pH7.0, batch, temperature 298K
Resolution 2.90 Å R-free 0.292
4V62 Crystal Structure of cyanobacterial Photosystem II Deposited 2008-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain BJ 1–40（40 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 3 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 11 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 7 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 CL CHLORIDE ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 7 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG2000, PIPES, magnesium chloride, pH7.0, batch, temperature 298K
Resolution 2.90 Å R-free 0.292
4V82 Crystal structure of cyanobacterial Photosystem II in complex with terbutryn Deposited 2010-11-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain AJ 1–40（40 aa）
Chain BJ 1–40（40 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 70 MST 2-T-BUTYLAMINO-4-ETHYLAMINO-6-METHYLTHIO-S-TRIAZINE × 2 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 LMT DODECYL-BETA-D-MALTOSIDE × 16 DMS DIMETHYL SULFOXIDE × 8 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；Pipes, CaCl2, DMSO, PEG 2000, pH 7.0, batch, temperature 298K
Resolution 3.20 Å R-free 0.299
5E79 Macromolecular diffractive imaging using imperfect crystals Deposited 2015-10-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain J 3–40（38 aa）
Chain j 3–40（38 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 CA CALCIUM ION × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；283.18 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 3.50 Å R-free 0.331
5E7C Macromolecular diffractive imaging using imperfect crystals - Bragg data Deposited 2015-10-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain J 3–40（38 aa）
Chain j 3–40（38 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 FE2 FE (II) ION × 2 CA CALCIUM ION × 6 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 4.50 Å R-free 0.275
5H2F Crystal structure of the PsbM-deletion mutant of photosystem II Deposited 2016-10-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain J 1–40（40 aa） Fragment：UNP RESIDUES 1-40
Not recorded OEX CA-MN4-O5 CLUSTER × 1 FE2 FE (II) ION × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 3 LMT DODECYL-BETA-D-MALTOSIDE × 6 UNL UNKNOWN LIGAND × 13 DMS DIMETHYL SULFOXIDE × 67 BCT BICARBONATE ION × 1 CA CALCIUM ION × 2 HTG heptyl 1-thio-beta-D-glucopyranoside × 9 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6.1；285 K；PEG1450, sodium chloride, calcium chloride, magnesium sulfate
Resolution 2.20 Å R-free 0.226
5H2F Crystal structure of the PsbM-deletion mutant of photosystem II Deposited 2016-10-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain j 1–40（40 aa） Fragment：UNP RESIDUES 1-40
Not recorded OEX CA-MN4-O5 CLUSTER × 1 FE2 FE (II) ION × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 5 LMT DODECYL-BETA-D-MALTOSIDE × 5 UNL UNKNOWN LIGAND × 14 DMS DIMETHYL SULFOXIDE × 66 BCT BICARBONATE ION × 1 CA CALCIUM ION × 3 HTG heptyl 1-thio-beta-D-glucopyranoside × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6.1；285 K；PEG1450, sodium chloride, calcium chloride, magnesium sulfate
Resolution 2.20 Å R-free 0.226
5KAI NH3-bound RT XFEL structure of Photosystem II 500 ms after the 2nd illumination (2F) at 2.8 A resolution Deposited 2016-06-01 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain J 1–40（40 aa）
Chain j 1–40（40 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 UNL UNKNOWN LIGAND × 23 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol
Resolution 2.80 Å R-free 0.300
5MX2 Photosystem II depleted of the Mn4CaO5 cluster at 2.55 A resolution Deposited 2017-01-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain J 1–40（40 aa）
Not recorded CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 11 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LFA EICOSANE × 19 FE FE (III) ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 4 BCT BICARBONATE ION × 1 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 7.5；291 K；equal volumes of buffer: 0.1 M Tris-HCl pH 7.5, 0.1 M (NH4)2SO4, 14-18 % PEG 5000 MME and protein: 2 mM chlorophyll equivalent of PSII in C12E8 detergent, post crystallisation treatment
Resolution 2.20 Å R-free 0.260
5MX2 Photosystem II depleted of the Mn4CaO5 cluster at 2.55 A resolution Deposited 2017-01-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain j 1–40（40 aa）
Not recorded CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 11 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LFA EICOSANE × 20 FE FE (III) ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 4 BCT BICARBONATE ION × 1 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 7.5；291 K；equal volumes of buffer: 0.1 M Tris-HCl pH 7.5, 0.1 M (NH4)2SO4, 14-18 % PEG 5000 MME and protein: 2 mM chlorophyll equivalent of PSII in C12E8 detergent, post crystallisation treatment
Resolution 2.20 Å R-free 0.260
5TIS Room temperature XFEL structure of the native, doubly-illuminated photosystem II complex Deposited 2016-10-03 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain J 1–40（40 aa）
Chain j 1–40（40 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 UNL UNKNOWN LIGAND × 33 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol
Resolution 2.25 Å R-free 0.231
5ZZN Crystal structure of photosystem II from an SQDG-deficient mutant of Thermosynechococcus elongatus Deposited 2018-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain J 1–40（40 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 5 UNL UNKNOWN LIGAND × 27 LMT DODECYL-BETA-D-MALTOSIDE × 9 DMS DIMETHYL SULFOXIDE × 48 CA CALCIUM ION × 3 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 HTG heptyl 1-thio-beta-D-glucopyranoside × 12 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 GOL GLYCEROL × 2 FE2 FE (II) ION × 1 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20MM NACL, 10MM CACL2, 40MM MGSO4, 20MM MES BUFFER, PH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPERATURE 285K
Resolution 2.10 Å R-free 0.197
5ZZN Crystal structure of photosystem II from an SQDG-deficient mutant of Thermosynechococcus elongatus Deposited 2018-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain j 1–40（40 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 1 CL CHLORIDE ION × 3 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 5 UNL UNKNOWN LIGAND × 25 LMT DODECYL-BETA-D-MALTOSIDE × 10 DMS DIMETHYL SULFOXIDE × 47 CA CALCIUM ION × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 HTG heptyl 1-thio-beta-D-glucopyranoside × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 GOL GLYCEROL × 3 FE2 FE (II) ION × 1 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20MM NACL, 10MM CACL2, 40MM MGSO4, 20MM MES BUFFER, PH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPERATURE 285K
Resolution 2.10 Å R-free 0.197
6DHE RT XFEL structure of the dark-stable state of Photosystem II (0F, S1-rich) at 2.05 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain J 5–40（36 aa）
Chain j 5–40（36 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 8CT (6'R,11cis,11'cis,13cis,15cis)-4',5'-didehydro-5',6'-dihydro-beta,beta-carotene × 12 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 BCR BETA-CAROTENE × 10 UNL UNKNOWN LIGAND × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.05 Å R-free 0.241
6DHF RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.08 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain J 5–40（36 aa）
Chain j 5–40（36 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 UNL UNKNOWN LIGAND × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.247
6DHG RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.5 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain J 5–40（36 aa）
Chain j 5–40（36 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.50 Å R-free 0.246
6DHH RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.2 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain J 5–40（36 aa）
Chain j 5–40（36 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.20 Å R-free 0.264
6DHO RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.07 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain J 5–40（36 aa）
Chain j 5–40（36 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.07 Å R-free 0.247
6DHP RT XFEL structure of the three-flash state of Photosystem II (3F, S0-rich) at 2.04 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain J 5–40（36 aa）
Chain j 5–40（36 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 UNL UNKNOWN LIGAND × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.04 Å R-free 0.248
6W1O RT XFEL structure of the dark-stable state of Photosystem II (0F, S1-rich) at 2.08 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain J 1–40（40 aa）
Chain j 1–40（40 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.240
6W1P RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.26 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain J 1–40（40 aa）
Chain j 1–40（40 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.26 Å R-free 0.241
6W1Q RT XFEL structure of Photosystem II 50 microseconds after the second illumination at 2.27 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain J 1–40（40 aa）
Chain j 1–40（40 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 BCR BETA-CAROTENE × 22 STE STEARIC ACID × 35 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 HEC HEME C × 4 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.27 Å R-free 0.249
6W1R RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.23 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain J 1–40（40 aa）
Chain j 1–40（40 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.23 Å R-free 0.235
6W1T RT XFEL structure of Photosystem II 250 microseconds after the second illumination at 2.01 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain J 1–40（40 aa）
Chain j 1–40（40 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.01 Å R-free 0.228
6W1U RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.09 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain J 1–40（40 aa）
Chain j 1–40（40 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
6W1V RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain J 1–40（40 aa）
Chain j 1–40（40 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 STE STEARIC ACID × 38 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 PHO PHEOPHYTIN A × 4 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.241
7RF1 RT XFEL structure of Photosystem II averaged across all S-states at 1.89 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain J 1–40（40 aa）
Chain j 1–40（40 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 STE STEARIC ACID × 55 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 1.89 Å R-free 0.214
7RF2 RT XFEL structure of dark-stable state of Photosystem II (0F, S1 rich) at 2.08 Angstrom Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain J 1–40（40 aa）
Chain j 1–40（40 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 33 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.238
7RF3 RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.26 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain J 1–40（40 aa）
Chain j 1–40（40 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.26 Å R-free 0.238
7RF4 RT XFEL structure of Photosystem II 50 microseconds after the second illumination at 2.27 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain J 1–40（40 aa）
Chain j 1–40（40 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 BCR BETA-CAROTENE × 22 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.27 Å R-free 0.244
7RF5 RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.23 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain J 1–40（40 aa）
Chain j 1–40（40 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.23 Å R-free 0.233
7RF6 RT XFEL structure of Photosystem II 250 microseconds after the second illumination at 2.01 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain J 1–40（40 aa）
Chain j 1–40（40 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.01 Å R-free 0.227
7RF7 RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.09 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain J 1–40（40 aa）
Chain j 1–40（40 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
7RF8 RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain J 1–40（40 aa）
Chain j 1–40（40 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
7YQ2 Crystal structure of photosystem II expressing psbA2 gene only Deposited 2022-08-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 39 PDB declaration: 39-meric
Chain J 1–40（40 aa）
Chain j 1–40（40 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 UNL UNKNOWN LIGAND × 77 LMT DODECYL-BETA-D-MALTOSIDE × 19 GOL GLYCEROL × 10 BCT BICARBONATE ION × 3 DMS DIMETHYL SULFOXIDE × 116 CA CALCIUM ION × 7 HTG heptyl 1-thio-beta-D-glucopyranoside × 31 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 9 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 MG MAGNESIUM ION × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20mM NaCl, 10mM CaCl2, 40mM MgSO4, 20mM Mes BUFFER, pH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPARATURE 285K
Resolution 1.90 Å R-free 0.184
7YQ7 Crystal structure of photosystem II expressing psbA3 gene only Deposited 2022-08-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 39 PDB declaration: 39-meric
Chain J 1–40（40 aa）
Chain j 1–40（40 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 UNL UNKNOWN LIGAND × 82 GOL GLYCEROL × 11 LMT DODECYL-BETA-D-MALTOSIDE × 18 BCT BICARBONATE ION × 4 DMS DIMETHYL SULFOXIDE × 71 CA CALCIUM ION × 5 HTG heptyl 1-thio-beta-D-glucopyranoside × 18 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 MG MAGNESIUM ION × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20mM NaCl, 10mM CaCl2, 40mM MgSO4, 20mM MES BUFFER pH 6.0, MICRO-BATCH METHOD UNDER OIL, TMPERATURE 285K
Resolution 1.90 Å R-free 0.177
8EZ5 RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2022-10-31 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain J 1–40（40 aa）
Chain j 1–40（40 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.234
8F4C RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain J 1–40（40 aa）
Chain j 1–40（40 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.224
8F4D RT XFEL structure of Photosystem II 50 microseconds after the third illumination at 2.15 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain J 1–40（40 aa）
Chain j 1–40（40 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.15 Å R-free 0.243
8F4E RT XFEL structure of Photosystem II 250 microseconds after the third illumination at 2.09 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain J 1–40（40 aa）
Chain j 1–40（40 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.233
8F4F RT XFEL structure of Photosystem II 500 microseconds after the third illumination at 2.03 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain J 1–40（40 aa）
Chain j 1–40（40 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.03 Å R-free 0.248
8F4G RT XFEL structure of Photosystem II 730 microseconds after the third illumination at 2.03 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain J 1–40（40 aa）
Chain j 1–40（40 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.03 Å R-free 0.223
8F4H RT XFEL structure of Photosystem II 1200 microseconds after the third illumination at 2.10 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain J 1–40（40 aa）
Chain j 1–40（40 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.10 Å R-free 0.232
8F4I RT XFEL structure of Photosystem II 2000 microseconds after the third illumination at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain J 1–40（40 aa）
Chain j 1–40（40 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.232
8F4J RT XFEL structure of Photosystem II 4000 microseconds after the third illumination at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain J 1–40（40 aa）
Chain j 1–40（40 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.226
8F4K RT XFEL structure of the three-flash state of Photosystem II (3F, S0-rich) at 2.16 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain J 1–40（40 aa）
Chain j 1–40（40 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.16 Å R-free 0.241
9EVX cryoEM structure of Photosystem II averaged across S2-S3 states at 1.71 Angstrom resolution Deposited 2024-04-02 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain J 1–40（40 aa）
Chain j 1–40（40 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 STE STEARIC ACID × 42 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 PHO PHEOPHYTIN A × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；100mM MES pH6.5, 5mM CaCl2, 5% glycerol, 0.03% DDM
cryo-EM vitrification conditions Cryogen ETHANE；The sample-on-grid was illuminated with one and two flashes of light
Resolution 1.71 Å
9G6F Inactive PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain J 1–40（40 aa）
Chain j 1–40（40 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LFA EICOSANE × 33 PLM PALMITIC ACID × 24 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 8 LMT DODECYL-BETA-D-MALTOSIDE × 13 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å
9G6G Semi-active PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric
Chain J 1–40（40 aa）
Chain j 1–40（40 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 3 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 LFA EICOSANE × 30 OEX CA-MN4-O5 CLUSTER × 1 LMT DODECYL-BETA-D-MALTOSIDE × 17 BCT BICARBONATE ION × 2 PLM PALMITIC ACID × 29 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 GOL GLYCEROL × 1 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 3 RRX (3R)-beta,beta-caroten-3-ol × 2 CA CALCIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å
9G6H active PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain J 1–40（40 aa）
Chain j 1–40（40 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 PLM PALMITIC ACID × 35 LFA EICOSANE × 24 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 GOL GLYCEROL × 2 LMT DODECYL-BETA-D-MALTOSIDE × 17 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 RRX (3R)-beta,beta-caroten-3-ol × 2 CA CALCIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å

60 other PDB entries and 65 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PSBJ_THEEB
Isoform —
PDB entities 9
Chains and sequence ranges Author chain J； PDBConstruct 1–40； UniProt 1–40 Author chain j； PDBConstruct 1–40； UniProt 1–40

Photosystem II reaction center protein K

OrganismNot specified

UniProt Q9F1K9

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 40 PDB declaration: 40-meric（40） Consistent with protein copy count Chain K； UniProt 1–46 Chain k； UniProt 1–46 Not recorded Photosystem II protein D1 1 × 2 （P0A444） Photosystem II CP47 reaction center protein × 2 （Q8DIQ1） Photosystem II CP43 reaction center protein × 2 （Q8DIF8） Photosystem II D2 protein × 2 （Q8CM25） Cytochrome b559 subunit alpha × 2 （Q8DIP0） Cytochrome b559 subunit beta × 2 （Q8DIN9） Photosystem II reaction center protein H × 2 （Q8DJ43） Photosystem II reaction center protein I × 2 （Q8DJZ6） Photosystem II reaction center protein J × 2 （P59087） Photosystem II reaction center protein L × 2 （Q8DIN8） Photosystem II reaction center protein M × 2 （Q8DHA7） Photosystem II manganese-stabilizing polypeptide × 2 （P0A431） Photosystem II reaction center protein T × 2 （Q8DIQ0） Photosystem II 12 kDa extrinsic protein × 2 （Q9F1L5） Cytochrome c-550 × 2 （P0A386） Photosystem II reaction center protein Ycf12 × 2 （Q8DJI1） Photosystem II reaction center X protein × 2 （Q9F1R6） Photosystem II reaction center protein Z × 2 （Q8DHJ2） Photosystem II protein Y × 2 （Q8DKM3） OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 UNL UNKNOWN LIGAND × 17 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION X-ray crystallization conditions：BATCH MODE；pH 7.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol. Resolution 3.00 Å R-free 0.303

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5KAF Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
11YO Single-conformation model re-refinement of 2F/S3-rich PSII intermediate structure at 2.09 Angstrom resolution Deposited 2026-03-18 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain K 1–46（46 aa）
Chain k 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 38 OEX CA-MN4-O5 CLUSTER × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 PHO PHEOPHYTIN A × 4 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.243
1IZL Crystal Structure of Photosystem II Deposited 2002-10-04 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain K 10–46（37 aa）
Chain W 10–46（37 aa）
Not recorded MN MANGANESE (II) ION × 8 CLA CHLOROPHYLL A × 72 PHO PHEOPHYTIN A × 4 FE FE (III) ION × 2 PLA 2-[(3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-YLMETHYL)-AMINO]-2-METHYL-SUCCINIC ACID × 2 BCR BETA-CAROTENE × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 X-RAY DIFFRACTION
X-ray crystallization conditions MICRODIALYSIS；pH 6.5；293 K；PEG 1450, MES, calcium chloride, magnesium sulfate, dodecyl maltoside, pH 6.5, MICRODIALYSIS, temperature 293.0K
Resolution 3.70 Å
1S5L Architecture of the photosynthetic oxygen evolving center Deposited 2004-01-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain K 10–46（37 aa）
Not recorded FE FE (III) ION × 1 BCT BICARBONATE ION × 2 OEC OXYGEN EVOLVING SYSTEM × 1 CLA CHLOROPHYLL A × 36 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMT DODECYL-BETA-D-MALTOSIDE × 1 BCR BETA-CAROTENE × 7 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pseudo-batch hanging drop；pH 7.5；291 K；100mM HEPES, 100mM (NH4)2SO4, PEG 4000, C12E8, trimethyl lead acetate, pH 7.5, pseudo-batch hanging drop, temperature 291K
Resolution 3.50 Å R-free 0.342
1S5L Architecture of the photosynthetic oxygen evolving center Deposited 2004-01-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain k 10–46（37 aa）
Not recorded FE FE (III) ION × 1 BCT BICARBONATE ION × 2 OEC OXYGEN EVOLVING SYSTEM × 1 CLA CHLOROPHYLL A × 36 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMT DODECYL-BETA-D-MALTOSIDE × 1 BCR BETA-CAROTENE × 7 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pseudo-batch hanging drop；pH 7.5；291 K；100mM HEPES, 100mM (NH4)2SO4, PEG 4000, C12E8, trimethyl lead acetate, pH 7.5, pseudo-batch hanging drop, temperature 291K
Resolution 3.50 Å R-free 0.342
2AXT Crystal Structure of Photosystem II from Thermosynechococcus elongatus Deposited 2005-09-06 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 36 PDB declaration: 36-meric
Chain K 10–46（37 aa）
Chain k 10–46（37 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PQ9 5-[(2E,6E,10E,14E,18E,22E)-3,7,11,15,19,23,27-HEPTAMETHYLOCTACOSA-2,6,10,14,18,22,26-HEPTAENYL]-2,3-DIMETHYLBENZO-1,4-QUINONE × 4 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 22 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LMT DODECYL-BETA-D-MALTOSIDE × 6 MGE (1S)-2-(ALPHA-L-ALLOPYRANOSYLOXY)-1-[(TRIDECANOYLOXY)METHYL]ETHYL PALMITATE × 12 UNL UNKNOWN LIGAND × 34 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG 2000, PIPES, magnesium chloride, pH 7.0, batch, temperature 298K
Resolution 3.00 Å R-free 0.286
3KZI Crystal Structure of Monomeric Form of Cyanobacterial Photosystem II Deposited 2009-12-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain K 10–46（37 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 11 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 LMT DODECYL-BETA-D-MALTOSIDE × 7 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 BCT BICARBONATE ION × 1 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；291 K；PEG 400, PIPES, MES, CaCl2, pH 7, batch, temperature 291.0K
Resolution 3.60 Å R-free 0.308
4FBY fs X-ray diffraction of Photosystem II Deposited 2012-05-23 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain K 10–46（37 aa）
Chain c 10–46（37 aa）
Not recorded CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 OEC OXYGEN EVOLVING SYSTEM × 2 FE2 FE (II) ION × 2 BCR BETA-CAROTENE × 24 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 CL CHLORIDE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；298 K；4% PEG 2000, 5 mM CaCl2, 100 mM PIPES, pH 7.0, batch, temperature 298K
Resolution 6.56 Å R-free 0.385
4IXQ RT fs X-ray diffraction of Photosystem II, dark state Deposited 2013-01-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain K 1–46（46 aa）
Chain k 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 LMT DODECYL-BETA-D-MALTOSIDE × 14 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.70 Å R-free 0.314
4IXR RT fs X-ray diffraction of Photosystem II, first illuminated state Deposited 2013-01-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain K 1–46（46 aa）
Chain k 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 LMT DODECYL-BETA-D-MALTOSIDE × 14 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.90 Å R-free 0.313
4PBU Serial Time-resolved crystallography of Photosystem II using a femtosecond X-ray laser The S1 state Deposited 2014-04-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain K 10–46（37 aa）
Chain k 10–46（37 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 CA CALCIUM ION × 7 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 5.00 Å R-free 0.262
4PJ0 Structure of T.elongatus Photosystem II, rows of dimers crystal packing Deposited 2014-05-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain K 1–46（46 aa）
Chain k 1–46（46 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE FE (III) ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SO4 SULFATE ION × 10 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 29 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；pH 7.5；293 K；Batch crystallisation: 2 mM Chl a equivalent of PSIIcc mixed 1:1 with: 0.1 M TRIS (pH 7.5), 0.1 M (NH4)2SO4, 15 - 18 % PEG 5000 MME, post-crystallisation dehydration
Resolution 2.44 Å R-free 0.256
4RVY Serial Time resolved crystallography of Photosystem II using a femtosecond X-ray laser. The S state after two flashes (S3) Deposited 2014-11-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain K 10–46（37 aa）
Chain k 10–46（37 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 CA CALCIUM ION × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions Free interface diffusion；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000 , Free interface diffusion, temperature 283K, temperature 283.0K
Resolution 5.50 Å R-free 0.291
4TNH RT XFEL structure of Photosystem II in the dark state at 4.9 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain K 1–46（46 aa）
Chain k 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.90 Å R-free 0.292
4TNI RT XFEL structure of Photosystem II 500 ms after the third illumination at 4.6 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain K 1–46（46 aa）
Chain k 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 2 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.60 Å R-free 0.284
4TNJ RT XFEL structure of Photosystem II 500 ms after the 2nd illumination (2F) at 4.5 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain K 1–46（46 aa）
Chain k 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.50 Å R-free 0.284
4TNK RT XFEL structure of Photosystem II 250 microsec after the third illumination at 5.2 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain K 1–46（46 aa）
Chain k 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.20 Å R-free 0.289
4V62 Crystal Structure of cyanobacterial Photosystem II Deposited 2008-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain AK 10–46（37 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 3 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 13 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 7 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 CL CHLORIDE ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 7 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG2000, PIPES, magnesium chloride, pH7.0, batch, temperature 298K
Resolution 2.90 Å R-free 0.292
4V62 Crystal Structure of cyanobacterial Photosystem II Deposited 2008-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain BK 10–46（37 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 3 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 11 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 7 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 CL CHLORIDE ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 7 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG2000, PIPES, magnesium chloride, pH7.0, batch, temperature 298K
Resolution 2.90 Å R-free 0.292
4V82 Crystal structure of cyanobacterial Photosystem II in complex with terbutryn Deposited 2010-11-30 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain AK 10–46（37 aa）
Chain BK 10–46（37 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 70 MST 2-T-BUTYLAMINO-4-ETHYLAMINO-6-METHYLTHIO-S-TRIAZINE × 2 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 LMT DODECYL-BETA-D-MALTOSIDE × 16 DMS DIMETHYL SULFOXIDE × 8 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；Pipes, CaCl2, DMSO, PEG 2000, pH 7.0, batch, temperature 298K
Resolution 3.20 Å R-free 0.299
5E79 Macromolecular diffractive imaging using imperfect crystals Deposited 2015-10-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain K 10–46（37 aa）
Chain k 10–46（37 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 CA CALCIUM ION × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；283.18 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 3.50 Å R-free 0.331
5E7C Macromolecular diffractive imaging using imperfect crystals - Bragg data Deposited 2015-10-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain K 10–46（37 aa）
Chain k 10–46（37 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 FE2 FE (II) ION × 2 CA CALCIUM ION × 6 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 4.50 Å R-free 0.275
5H2F Crystal structure of the PsbM-deletion mutant of photosystem II Deposited 2016-10-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain K 10–46（37 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 1 FE2 FE (II) ION × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 3 LMT DODECYL-BETA-D-MALTOSIDE × 6 UNL UNKNOWN LIGAND × 13 DMS DIMETHYL SULFOXIDE × 67 BCT BICARBONATE ION × 1 CA CALCIUM ION × 2 HTG heptyl 1-thio-beta-D-glucopyranoside × 9 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6.1；285 K；PEG1450, sodium chloride, calcium chloride, magnesium sulfate
Resolution 2.20 Å R-free 0.226
5H2F Crystal structure of the PsbM-deletion mutant of photosystem II Deposited 2016-10-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain k 10–46（37 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 1 FE2 FE (II) ION × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 5 LMT DODECYL-BETA-D-MALTOSIDE × 5 UNL UNKNOWN LIGAND × 14 DMS DIMETHYL SULFOXIDE × 66 BCT BICARBONATE ION × 1 CA CALCIUM ION × 3 HTG heptyl 1-thio-beta-D-glucopyranoside × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6.1；285 K；PEG1450, sodium chloride, calcium chloride, magnesium sulfate
Resolution 2.20 Å R-free 0.226
5KAI NH3-bound RT XFEL structure of Photosystem II 500 ms after the 2nd illumination (2F) at 2.8 A resolution Deposited 2016-06-01 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain K 1–46（46 aa）
Chain k 1–46（46 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 UNL UNKNOWN LIGAND × 23 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol
Resolution 2.80 Å R-free 0.300
5MX2 Photosystem II depleted of the Mn4CaO5 cluster at 2.55 A resolution Deposited 2017-01-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain K 1–46（46 aa）
Not recorded CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 11 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LFA EICOSANE × 19 FE FE (III) ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 4 BCT BICARBONATE ION × 1 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 7.5；291 K；equal volumes of buffer: 0.1 M Tris-HCl pH 7.5, 0.1 M (NH4)2SO4, 14-18 % PEG 5000 MME and protein: 2 mM chlorophyll equivalent of PSII in C12E8 detergent, post crystallisation treatment
Resolution 2.20 Å R-free 0.260
5MX2 Photosystem II depleted of the Mn4CaO5 cluster at 2.55 A resolution Deposited 2017-01-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain k 1–46（46 aa）
Not recorded CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 11 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LFA EICOSANE × 20 FE FE (III) ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 4 BCT BICARBONATE ION × 1 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 7.5；291 K；equal volumes of buffer: 0.1 M Tris-HCl pH 7.5, 0.1 M (NH4)2SO4, 14-18 % PEG 5000 MME and protein: 2 mM chlorophyll equivalent of PSII in C12E8 detergent, post crystallisation treatment
Resolution 2.20 Å R-free 0.260
5TIS Room temperature XFEL structure of the native, doubly-illuminated photosystem II complex Deposited 2016-10-03 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain K 1–46（46 aa）
Chain k 1–46（46 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 UNL UNKNOWN LIGAND × 33 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol
Resolution 2.25 Å R-free 0.231
5ZZN Crystal structure of photosystem II from an SQDG-deficient mutant of Thermosynechococcus elongatus Deposited 2018-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain K 10–46（37 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 5 UNL UNKNOWN LIGAND × 27 LMT DODECYL-BETA-D-MALTOSIDE × 9 DMS DIMETHYL SULFOXIDE × 48 CA CALCIUM ION × 3 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 HTG heptyl 1-thio-beta-D-glucopyranoside × 12 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 GOL GLYCEROL × 2 FE2 FE (II) ION × 1 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20MM NACL, 10MM CACL2, 40MM MGSO4, 20MM MES BUFFER, PH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPERATURE 285K
Resolution 2.10 Å R-free 0.197
5ZZN Crystal structure of photosystem II from an SQDG-deficient mutant of Thermosynechococcus elongatus Deposited 2018-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain k 10–46（37 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 1 CL CHLORIDE ION × 3 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 5 UNL UNKNOWN LIGAND × 25 LMT DODECYL-BETA-D-MALTOSIDE × 10 DMS DIMETHYL SULFOXIDE × 47 CA CALCIUM ION × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 HTG heptyl 1-thio-beta-D-glucopyranoside × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 GOL GLYCEROL × 3 FE2 FE (II) ION × 1 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20MM NACL, 10MM CACL2, 40MM MGSO4, 20MM MES BUFFER, PH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPERATURE 285K
Resolution 2.10 Å R-free 0.197
6DHE RT XFEL structure of the dark-stable state of Photosystem II (0F, S1-rich) at 2.05 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain K 10–46（37 aa）
Chain k 10–46（37 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 8CT (6'R,11cis,11'cis,13cis,15cis)-4',5'-didehydro-5',6'-dihydro-beta,beta-carotene × 12 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 BCR BETA-CAROTENE × 10 UNL UNKNOWN LIGAND × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.05 Å R-free 0.241
6DHF RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.08 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain K 10–46（37 aa）
Chain k 10–46（37 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 UNL UNKNOWN LIGAND × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.247
6DHG RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.5 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain K 10–46（37 aa）
Chain k 10–46（37 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.50 Å R-free 0.246
6DHH RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.2 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain K 10–46（37 aa）
Chain k 10–46（37 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.20 Å R-free 0.264
6DHO RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.07 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain K 10–46（37 aa）
Chain k 10–46（37 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.07 Å R-free 0.247
6DHP RT XFEL structure of the three-flash state of Photosystem II (3F, S0-rich) at 2.04 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain K 10–46（37 aa）
Chain k 10–46（37 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 UNL UNKNOWN LIGAND × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.04 Å R-free 0.248
6W1O RT XFEL structure of the dark-stable state of Photosystem II (0F, S1-rich) at 2.08 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain K 1–46（46 aa）
Chain k 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.240
6W1P RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.26 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain K 1–46（46 aa）
Chain k 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.26 Å R-free 0.241
6W1Q RT XFEL structure of Photosystem II 50 microseconds after the second illumination at 2.27 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain K 1–46（46 aa）
Chain k 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 BCR BETA-CAROTENE × 22 STE STEARIC ACID × 35 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 HEC HEME C × 4 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.27 Å R-free 0.249
6W1R RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.23 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain K 1–46（46 aa）
Chain k 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.23 Å R-free 0.235
6W1T RT XFEL structure of Photosystem II 250 microseconds after the second illumination at 2.01 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain K 1–46（46 aa）
Chain k 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.01 Å R-free 0.228
6W1U RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.09 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain K 1–46（46 aa）
Chain k 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
6W1V RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain K 1–46（46 aa）
Chain k 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 STE STEARIC ACID × 38 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 PHO PHEOPHYTIN A × 4 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.241
7DXH Cryo-EM structure of PSII intermediate Psb28-PSII complex Deposited 2021-01-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain k 1–46（46 aa）
Not recorded MGE (1S)-2-(ALPHA-L-ALLOPYRANOSYLOXY)-1-[(TRIDECANOYLOXY)METHYL]ETHYL PALMITATE × 7 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 2 CLA CHLOROPHYLL A × 34 BCR BETA-CAROTENE × 9 CL CHLORIDE ION × 2 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 4 PHO PHEOPHYTIN A × 2 LMT DODECYL-BETA-D-MALTOSIDE × 3 FE2 FE (II) ION × 1 PQ9 5-[(2E,6E,10E,14E,18E,22E)-3,7,11,15,19,23,27-HEPTAMETHYLOCTACOSA-2,6,10,14,18,22,26-HEPTAENYL]-2,3-DIMETHYLBENZO-1,4-QUINONE × 1 UNL UNKNOWN LIGAND × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 HTG heptyl 1-thio-beta-D-glucopyranoside × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.14 Å
7NHO Structure of PSII-M Deposited 2021-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain K 1–46（46 aa）
Not recorded FE FE (III) ION × 1 MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 BCT BICARBONATE ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.66 Å
7NHP Structure of PSII-I (PSII with Psb27, Psb28, and Psb34) Deposited 2021-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain K 1–46（46 aa）
Not recorded MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 FE FE (III) ION × 1 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.72 Å
7NHQ Structure of PSII-I prime (PSII with Psb28, and Psb34) Deposited 2021-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 17 PDB declaration: heptadecameric
Chain K 1–46（46 aa）
Not recorded FE FE (III) ION × 1 MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.68 Å
7RF1 RT XFEL structure of Photosystem II averaged across all S-states at 1.89 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain K 1–46（46 aa）
Chain k 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 STE STEARIC ACID × 55 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 1.89 Å R-free 0.214
7RF2 RT XFEL structure of dark-stable state of Photosystem II (0F, S1 rich) at 2.08 Angstrom Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain K 1–46（46 aa）
Chain k 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 33 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.238
7RF3 RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.26 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain K 1–46（46 aa）
Chain k 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.26 Å R-free 0.238
7RF4 RT XFEL structure of Photosystem II 50 microseconds after the second illumination at 2.27 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain K 1–46（46 aa）
Chain k 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 BCR BETA-CAROTENE × 22 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.27 Å R-free 0.244
7RF5 RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.23 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain K 1–46（46 aa）
Chain k 1–46（46 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.23 Å R-free 0.233
7RF6 RT XFEL structure of Photosystem II 250 microseconds after the second illumination at 2.01 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain K 1–46（46 aa）
Chain k 1–46（46 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.01 Å R-free 0.227
7RF7 RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.09 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain K 1–46（46 aa）
Chain k 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
7RF8 RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain K 1–46（46 aa）
Chain k 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
7YQ2 Crystal structure of photosystem II expressing psbA2 gene only Deposited 2022-08-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 39 PDB declaration: 39-meric
Chain K 1–46（46 aa）
Chain k 1–46（46 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 UNL UNKNOWN LIGAND × 77 LMT DODECYL-BETA-D-MALTOSIDE × 19 GOL GLYCEROL × 10 BCT BICARBONATE ION × 3 DMS DIMETHYL SULFOXIDE × 116 CA CALCIUM ION × 7 HTG heptyl 1-thio-beta-D-glucopyranoside × 31 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 9 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 MG MAGNESIUM ION × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20mM NaCl, 10mM CaCl2, 40mM MgSO4, 20mM Mes BUFFER, pH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPARATURE 285K
Resolution 1.90 Å R-free 0.184
7YQ7 Crystal structure of photosystem II expressing psbA3 gene only Deposited 2022-08-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 39 PDB declaration: 39-meric
Chain K 1–46（46 aa）
Chain k 1–46（46 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 UNL UNKNOWN LIGAND × 82 GOL GLYCEROL × 11 LMT DODECYL-BETA-D-MALTOSIDE × 18 BCT BICARBONATE ION × 4 DMS DIMETHYL SULFOXIDE × 71 CA CALCIUM ION × 5 HTG heptyl 1-thio-beta-D-glucopyranoside × 18 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 MG MAGNESIUM ION × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20mM NaCl, 10mM CaCl2, 40mM MgSO4, 20mM MES BUFFER pH 6.0, MICRO-BATCH METHOD UNDER OIL, TMPERATURE 285K
Resolution 1.90 Å R-free 0.177
8EZ5 RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2022-10-31 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain K 1–46（46 aa）
Chain k 1–46（46 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.234
8F4C RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain K 1–46（46 aa）
Chain k 1–46（46 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.224
8F4D RT XFEL structure of Photosystem II 50 microseconds after the third illumination at 2.15 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain K 1–46（46 aa）
Chain k 1–46（46 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.15 Å R-free 0.243
8F4E RT XFEL structure of Photosystem II 250 microseconds after the third illumination at 2.09 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain K 1–46（46 aa）
Chain k 1–46（46 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.233
8F4F RT XFEL structure of Photosystem II 500 microseconds after the third illumination at 2.03 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain K 1–46（46 aa）
Chain k 1–46（46 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.03 Å R-free 0.248
8F4G RT XFEL structure of Photosystem II 730 microseconds after the third illumination at 2.03 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain K 1–46（46 aa）
Chain k 1–46（46 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.03 Å R-free 0.223
8F4H RT XFEL structure of Photosystem II 1200 microseconds after the third illumination at 2.10 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain K 1–46（46 aa）
Chain k 1–46（46 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.10 Å R-free 0.232
8F4I RT XFEL structure of Photosystem II 2000 microseconds after the third illumination at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain K 1–46（46 aa）
Chain k 1–46（46 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.232
8F4J RT XFEL structure of Photosystem II 4000 microseconds after the third illumination at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain K 1–46（46 aa）
Chain k 1–46（46 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.226
8F4K RT XFEL structure of the three-flash state of Photosystem II (3F, S0-rich) at 2.16 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain K 1–46（46 aa）
Chain k 1–46（46 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.16 Å R-free 0.241
9EVX cryoEM structure of Photosystem II averaged across S2-S3 states at 1.71 Angstrom resolution Deposited 2024-04-02 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain K 1–46（46 aa）
Chain k 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 STE STEARIC ACID × 42 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 PHO PHEOPHYTIN A × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；100mM MES pH6.5, 5mM CaCl2, 5% glycerol, 0.03% DDM
cryo-EM vitrification conditions Cryogen ETHANE；The sample-on-grid was illuminated with one and two flashes of light
Resolution 1.71 Å
9G6F Inactive PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain K 1–46（46 aa）
Chain k 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LFA EICOSANE × 33 PLM PALMITIC ACID × 24 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 8 LMT DODECYL-BETA-D-MALTOSIDE × 13 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å
9G6G Semi-active PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric
Chain K 1–46（46 aa）
Chain k 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 3 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 LFA EICOSANE × 30 OEX CA-MN4-O5 CLUSTER × 1 LMT DODECYL-BETA-D-MALTOSIDE × 17 BCT BICARBONATE ION × 2 PLM PALMITIC ACID × 29 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 GOL GLYCEROL × 1 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 3 RRX (3R)-beta,beta-caroten-3-ol × 2 CA CALCIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å
9G6H active PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain K 1–46（46 aa）
Chain k 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 PLM PALMITIC ACID × 35 LFA EICOSANE × 24 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 GOL GLYCEROL × 2 LMT DODECYL-BETA-D-MALTOSIDE × 17 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 RRX (3R)-beta,beta-caroten-3-ol × 2 CA CALCIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å

65 other PDB entries and 70 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PSBK_THEEB
Isoform —
PDB entities 10
Chains and sequence ranges Author chain K； PDBConstruct 1–46； UniProt 1–46 Author chain k； PDBConstruct 1–46； UniProt 1–46

Photosystem II reaction center protein L

OrganismNot specified

UniProt Q8DIN8

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 40 PDB declaration: 40-meric（40） Consistent with protein copy count Chain L； UniProt 1–37 Chain l； UniProt 1–37 Not recorded Photosystem II protein D1 1 × 2 （P0A444） Photosystem II CP47 reaction center protein × 2 （Q8DIQ1） Photosystem II CP43 reaction center protein × 2 （Q8DIF8） Photosystem II D2 protein × 2 （Q8CM25） Cytochrome b559 subunit alpha × 2 （Q8DIP0） Cytochrome b559 subunit beta × 2 （Q8DIN9） Photosystem II reaction center protein H × 2 （Q8DJ43） Photosystem II reaction center protein I × 2 （Q8DJZ6） Photosystem II reaction center protein J × 2 （P59087） Photosystem II reaction center protein K × 2 （Q9F1K9） Photosystem II reaction center protein M × 2 （Q8DHA7） Photosystem II manganese-stabilizing polypeptide × 2 （P0A431） Photosystem II reaction center protein T × 2 （Q8DIQ0） Photosystem II 12 kDa extrinsic protein × 2 （Q9F1L5） Cytochrome c-550 × 2 （P0A386） Photosystem II reaction center protein Ycf12 × 2 （Q8DJI1） Photosystem II reaction center X protein × 2 （Q9F1R6） Photosystem II reaction center protein Z × 2 （Q8DHJ2） Photosystem II protein Y × 2 （Q8DKM3） OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 UNL UNKNOWN LIGAND × 17 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION X-ray crystallization conditions：BATCH MODE；pH 7.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol. Resolution 3.00 Å R-free 0.303

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5KAF Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
11YO Single-conformation model re-refinement of 2F/S3-rich PSII intermediate structure at 2.09 Angstrom resolution Deposited 2026-03-18 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 38 OEX CA-MN4-O5 CLUSTER × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 PHO PHEOPHYTIN A × 4 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.243
1S5L Architecture of the photosynthetic oxygen evolving center Deposited 2004-01-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain L 1–37（37 aa）
Not recorded FE FE (III) ION × 1 BCT BICARBONATE ION × 2 OEC OXYGEN EVOLVING SYSTEM × 1 CLA CHLOROPHYLL A × 36 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMT DODECYL-BETA-D-MALTOSIDE × 1 BCR BETA-CAROTENE × 7 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pseudo-batch hanging drop；pH 7.5；291 K；100mM HEPES, 100mM (NH4)2SO4, PEG 4000, C12E8, trimethyl lead acetate, pH 7.5, pseudo-batch hanging drop, temperature 291K
Resolution 3.50 Å R-free 0.342
1S5L Architecture of the photosynthetic oxygen evolving center Deposited 2004-01-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain l 1–37（37 aa）
Not recorded FE FE (III) ION × 1 BCT BICARBONATE ION × 2 OEC OXYGEN EVOLVING SYSTEM × 1 CLA CHLOROPHYLL A × 36 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMT DODECYL-BETA-D-MALTOSIDE × 1 BCR BETA-CAROTENE × 7 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pseudo-batch hanging drop；pH 7.5；291 K；100mM HEPES, 100mM (NH4)2SO4, PEG 4000, C12E8, trimethyl lead acetate, pH 7.5, pseudo-batch hanging drop, temperature 291K
Resolution 3.50 Å R-free 0.342
2AXT Crystal Structure of Photosystem II from Thermosynechococcus elongatus Deposited 2005-09-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 36 PDB declaration: 36-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PQ9 5-[(2E,6E,10E,14E,18E,22E)-3,7,11,15,19,23,27-HEPTAMETHYLOCTACOSA-2,6,10,14,18,22,26-HEPTAENYL]-2,3-DIMETHYLBENZO-1,4-QUINONE × 4 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 22 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LMT DODECYL-BETA-D-MALTOSIDE × 6 MGE (1S)-2-(ALPHA-L-ALLOPYRANOSYLOXY)-1-[(TRIDECANOYLOXY)METHYL]ETHYL PALMITATE × 12 UNL UNKNOWN LIGAND × 34 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG 2000, PIPES, magnesium chloride, pH 7.0, batch, temperature 298K
Resolution 3.00 Å R-free 0.286
3KZI Crystal Structure of Monomeric Form of Cyanobacterial Photosystem II Deposited 2009-12-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain L 1–37（37 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 11 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 LMT DODECYL-BETA-D-MALTOSIDE × 7 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 BCT BICARBONATE ION × 1 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；291 K；PEG 400, PIPES, MES, CaCl2, pH 7, batch, temperature 291.0K
Resolution 3.60 Å R-free 0.308
4FBY fs X-ray diffraction of Photosystem II Deposited 2012-05-23 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain L 1–37（37 aa）
Chain d 1–37（37 aa）
Not recorded CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 OEC OXYGEN EVOLVING SYSTEM × 2 FE2 FE (II) ION × 2 BCR BETA-CAROTENE × 24 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 CL CHLORIDE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；298 K；4% PEG 2000, 5 mM CaCl2, 100 mM PIPES, pH 7.0, batch, temperature 298K
Resolution 6.56 Å R-free 0.385
4IXQ RT fs X-ray diffraction of Photosystem II, dark state Deposited 2013-01-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 LMT DODECYL-BETA-D-MALTOSIDE × 14 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.70 Å R-free 0.314
4IXR RT fs X-ray diffraction of Photosystem II, first illuminated state Deposited 2013-01-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 LMT DODECYL-BETA-D-MALTOSIDE × 14 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.90 Å R-free 0.313
4PBU Serial Time-resolved crystallography of Photosystem II using a femtosecond X-ray laser The S1 state Deposited 2014-04-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 CA CALCIUM ION × 7 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 5.00 Å R-free 0.262
4PJ0 Structure of T.elongatus Photosystem II, rows of dimers crystal packing Deposited 2014-05-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE FE (III) ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SO4 SULFATE ION × 10 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 29 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；pH 7.5；293 K；Batch crystallisation: 2 mM Chl a equivalent of PSIIcc mixed 1:1 with: 0.1 M TRIS (pH 7.5), 0.1 M (NH4)2SO4, 15 - 18 % PEG 5000 MME, post-crystallisation dehydration
Resolution 2.44 Å R-free 0.256
4RVY Serial Time resolved crystallography of Photosystem II using a femtosecond X-ray laser. The S state after two flashes (S3) Deposited 2014-11-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 CA CALCIUM ION × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions Free interface diffusion；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000 , Free interface diffusion, temperature 283K, temperature 283.0K
Resolution 5.50 Å R-free 0.291
4TNH RT XFEL structure of Photosystem II in the dark state at 4.9 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.90 Å R-free 0.292
4TNI RT XFEL structure of Photosystem II 500 ms after the third illumination at 4.6 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 2 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.60 Å R-free 0.284
4TNJ RT XFEL structure of Photosystem II 500 ms after the 2nd illumination (2F) at 4.5 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.50 Å R-free 0.284
4TNK RT XFEL structure of Photosystem II 250 microsec after the third illumination at 5.2 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.20 Å R-free 0.289
4V62 Crystal Structure of cyanobacterial Photosystem II Deposited 2008-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain AL 1–37（37 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 3 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 13 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 7 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 CL CHLORIDE ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 7 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG2000, PIPES, magnesium chloride, pH7.0, batch, temperature 298K
Resolution 2.90 Å R-free 0.292
4V62 Crystal Structure of cyanobacterial Photosystem II Deposited 2008-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain BL 1–37（37 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 3 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 11 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 7 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 CL CHLORIDE ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 7 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG2000, PIPES, magnesium chloride, pH7.0, batch, temperature 298K
Resolution 2.90 Å R-free 0.292
4V82 Crystal structure of cyanobacterial Photosystem II in complex with terbutryn Deposited 2010-11-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain AL 1–37（37 aa）
Chain BL 1–37（37 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 70 MST 2-T-BUTYLAMINO-4-ETHYLAMINO-6-METHYLTHIO-S-TRIAZINE × 2 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 LMT DODECYL-BETA-D-MALTOSIDE × 16 DMS DIMETHYL SULFOXIDE × 8 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；Pipes, CaCl2, DMSO, PEG 2000, pH 7.0, batch, temperature 298K
Resolution 3.20 Å R-free 0.299
5E79 Macromolecular diffractive imaging using imperfect crystals Deposited 2015-10-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 CA CALCIUM ION × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；283.18 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 3.50 Å R-free 0.331
5E7C Macromolecular diffractive imaging using imperfect crystals - Bragg data Deposited 2015-10-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 FE2 FE (II) ION × 2 CA CALCIUM ION × 6 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 4.50 Å R-free 0.275
5H2F Crystal structure of the PsbM-deletion mutant of photosystem II Deposited 2016-10-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain L 3–37（35 aa） Fragment：UNP RESIDUES 3-37
Not recorded OEX CA-MN4-O5 CLUSTER × 1 FE2 FE (II) ION × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 3 LMT DODECYL-BETA-D-MALTOSIDE × 6 UNL UNKNOWN LIGAND × 13 DMS DIMETHYL SULFOXIDE × 67 BCT BICARBONATE ION × 1 CA CALCIUM ION × 2 HTG heptyl 1-thio-beta-D-glucopyranoside × 9 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6.1；285 K；PEG1450, sodium chloride, calcium chloride, magnesium sulfate
Resolution 2.20 Å R-free 0.226
5H2F Crystal structure of the PsbM-deletion mutant of photosystem II Deposited 2016-10-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain l 3–37（35 aa） Fragment：UNP RESIDUES 3-37
Not recorded OEX CA-MN4-O5 CLUSTER × 1 FE2 FE (II) ION × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 5 LMT DODECYL-BETA-D-MALTOSIDE × 5 UNL UNKNOWN LIGAND × 14 DMS DIMETHYL SULFOXIDE × 66 BCT BICARBONATE ION × 1 CA CALCIUM ION × 3 HTG heptyl 1-thio-beta-D-glucopyranoside × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6.1；285 K；PEG1450, sodium chloride, calcium chloride, magnesium sulfate
Resolution 2.20 Å R-free 0.226
5KAI NH3-bound RT XFEL structure of Photosystem II 500 ms after the 2nd illumination (2F) at 2.8 A resolution Deposited 2016-06-01 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 UNL UNKNOWN LIGAND × 23 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol
Resolution 2.80 Å R-free 0.300
5MX2 Photosystem II depleted of the Mn4CaO5 cluster at 2.55 A resolution Deposited 2017-01-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain L 1–37（37 aa）
Not recorded CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 11 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LFA EICOSANE × 19 FE FE (III) ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 4 BCT BICARBONATE ION × 1 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 7.5；291 K；equal volumes of buffer: 0.1 M Tris-HCl pH 7.5, 0.1 M (NH4)2SO4, 14-18 % PEG 5000 MME and protein: 2 mM chlorophyll equivalent of PSII in C12E8 detergent, post crystallisation treatment
Resolution 2.20 Å R-free 0.260
5MX2 Photosystem II depleted of the Mn4CaO5 cluster at 2.55 A resolution Deposited 2017-01-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain l 1–37（37 aa）
Not recorded CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 11 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LFA EICOSANE × 20 FE FE (III) ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 4 BCT BICARBONATE ION × 1 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 7.5；291 K；equal volumes of buffer: 0.1 M Tris-HCl pH 7.5, 0.1 M (NH4)2SO4, 14-18 % PEG 5000 MME and protein: 2 mM chlorophyll equivalent of PSII in C12E8 detergent, post crystallisation treatment
Resolution 2.20 Å R-free 0.260
5TIS Room temperature XFEL structure of the native, doubly-illuminated photosystem II complex Deposited 2016-10-03 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 UNL UNKNOWN LIGAND × 33 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol
Resolution 2.25 Å R-free 0.231
5ZZN Crystal structure of photosystem II from an SQDG-deficient mutant of Thermosynechococcus elongatus Deposited 2018-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain L 2–37（36 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 5 UNL UNKNOWN LIGAND × 27 LMT DODECYL-BETA-D-MALTOSIDE × 9 DMS DIMETHYL SULFOXIDE × 48 CA CALCIUM ION × 3 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 HTG heptyl 1-thio-beta-D-glucopyranoside × 12 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 GOL GLYCEROL × 2 FE2 FE (II) ION × 1 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20MM NACL, 10MM CACL2, 40MM MGSO4, 20MM MES BUFFER, PH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPERATURE 285K
Resolution 2.10 Å R-free 0.197
5ZZN Crystal structure of photosystem II from an SQDG-deficient mutant of Thermosynechococcus elongatus Deposited 2018-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain l 2–37（36 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 1 CL CHLORIDE ION × 3 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 5 UNL UNKNOWN LIGAND × 25 LMT DODECYL-BETA-D-MALTOSIDE × 10 DMS DIMETHYL SULFOXIDE × 47 CA CALCIUM ION × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 HTG heptyl 1-thio-beta-D-glucopyranoside × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 GOL GLYCEROL × 3 FE2 FE (II) ION × 1 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20MM NACL, 10MM CACL2, 40MM MGSO4, 20MM MES BUFFER, PH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPERATURE 285K
Resolution 2.10 Å R-free 0.197
6DHE RT XFEL structure of the dark-stable state of Photosystem II (0F, S1-rich) at 2.05 Angstrom resolution Deposited 2018-05-20 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 8CT (6'R,11cis,11'cis,13cis,15cis)-4',5'-didehydro-5',6'-dihydro-beta,beta-carotene × 12 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 BCR BETA-CAROTENE × 10 UNL UNKNOWN LIGAND × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.05 Å R-free 0.241
6DHF RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.08 Angstrom resolution Deposited 2018-05-20 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 UNL UNKNOWN LIGAND × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.247
6DHG RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.5 Angstrom resolution Deposited 2018-05-20 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.50 Å R-free 0.246
6DHH RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.2 Angstrom resolution Deposited 2018-05-20 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.20 Å R-free 0.264
6DHO RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.07 Angstrom resolution Deposited 2018-05-20 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.07 Å R-free 0.247
6DHP RT XFEL structure of the three-flash state of Photosystem II (3F, S0-rich) at 2.04 Angstrom resolution Deposited 2018-05-20 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 UNL UNKNOWN LIGAND × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.04 Å R-free 0.248
6W1O RT XFEL structure of the dark-stable state of Photosystem II (0F, S1-rich) at 2.08 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.240
6W1P RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.26 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.26 Å R-free 0.241
6W1Q RT XFEL structure of Photosystem II 50 microseconds after the second illumination at 2.27 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 BCR BETA-CAROTENE × 22 STE STEARIC ACID × 35 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 HEC HEME C × 4 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.27 Å R-free 0.249
6W1R RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.23 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.23 Å R-free 0.235
6W1T RT XFEL structure of Photosystem II 250 microseconds after the second illumination at 2.01 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.01 Å R-free 0.228
6W1U RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.09 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
6W1V RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 STE STEARIC ACID × 38 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 PHO PHEOPHYTIN A × 4 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.241
7NHO Structure of PSII-M Deposited 2021-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain L 1–37（37 aa）
Not recorded FE FE (III) ION × 1 MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 BCT BICARBONATE ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.66 Å
7NHP Structure of PSII-I (PSII with Psb27, Psb28, and Psb34) Deposited 2021-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain L 1–37（37 aa）
Not recorded MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 FE FE (III) ION × 1 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.72 Å
7NHQ Structure of PSII-I prime (PSII with Psb28, and Psb34) Deposited 2021-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 17 PDB declaration: heptadecameric
Chain L 1–37（37 aa）
Not recorded FE FE (III) ION × 1 MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.68 Å
7RF1 RT XFEL structure of Photosystem II averaged across all S-states at 1.89 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 STE STEARIC ACID × 55 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 1.89 Å R-free 0.214
7RF2 RT XFEL structure of dark-stable state of Photosystem II (0F, S1 rich) at 2.08 Angstrom Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 33 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.238
7RF3 RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.26 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.26 Å R-free 0.238
7RF4 RT XFEL structure of Photosystem II 50 microseconds after the second illumination at 2.27 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 BCR BETA-CAROTENE × 22 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.27 Å R-free 0.244
7RF5 RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.23 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.23 Å R-free 0.233
7RF6 RT XFEL structure of Photosystem II 250 microseconds after the second illumination at 2.01 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.01 Å R-free 0.227
7RF7 RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.09 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
7RF8 RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
7YQ2 Crystal structure of photosystem II expressing psbA2 gene only Deposited 2022-08-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 39 PDB declaration: 39-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 UNL UNKNOWN LIGAND × 77 LMT DODECYL-BETA-D-MALTOSIDE × 19 GOL GLYCEROL × 10 BCT BICARBONATE ION × 3 DMS DIMETHYL SULFOXIDE × 116 CA CALCIUM ION × 7 HTG heptyl 1-thio-beta-D-glucopyranoside × 31 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 9 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 MG MAGNESIUM ION × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20mM NaCl, 10mM CaCl2, 40mM MgSO4, 20mM Mes BUFFER, pH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPARATURE 285K
Resolution 1.90 Å R-free 0.184
7YQ7 Crystal structure of photosystem II expressing psbA3 gene only Deposited 2022-08-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 39 PDB declaration: 39-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 UNL UNKNOWN LIGAND × 82 GOL GLYCEROL × 11 LMT DODECYL-BETA-D-MALTOSIDE × 18 BCT BICARBONATE ION × 4 DMS DIMETHYL SULFOXIDE × 71 CA CALCIUM ION × 5 HTG heptyl 1-thio-beta-D-glucopyranoside × 18 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 MG MAGNESIUM ION × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20mM NaCl, 10mM CaCl2, 40mM MgSO4, 20mM MES BUFFER pH 6.0, MICRO-BATCH METHOD UNDER OIL, TMPERATURE 285K
Resolution 1.90 Å R-free 0.177
8EZ5 RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2022-10-31 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.234
8F4C RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.224
8F4D RT XFEL structure of Photosystem II 50 microseconds after the third illumination at 2.15 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.15 Å R-free 0.243
8F4E RT XFEL structure of Photosystem II 250 microseconds after the third illumination at 2.09 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.233
8F4F RT XFEL structure of Photosystem II 500 microseconds after the third illumination at 2.03 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.03 Å R-free 0.248
8F4G RT XFEL structure of Photosystem II 730 microseconds after the third illumination at 2.03 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.03 Å R-free 0.223
8F4H RT XFEL structure of Photosystem II 1200 microseconds after the third illumination at 2.10 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.10 Å R-free 0.232
8F4I RT XFEL structure of Photosystem II 2000 microseconds after the third illumination at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.232
8F4J RT XFEL structure of Photosystem II 4000 microseconds after the third illumination at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.226
8F4K RT XFEL structure of the three-flash state of Photosystem II (3F, S0-rich) at 2.16 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.16 Å R-free 0.241
9EVX cryoEM structure of Photosystem II averaged across S2-S3 states at 1.71 Angstrom resolution Deposited 2024-04-02 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 STE STEARIC ACID × 42 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 PHO PHEOPHYTIN A × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；100mM MES pH6.5, 5mM CaCl2, 5% glycerol, 0.03% DDM
cryo-EM vitrification conditions Cryogen ETHANE；The sample-on-grid was illuminated with one and two flashes of light
Resolution 1.71 Å
9G6F Inactive PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LFA EICOSANE × 33 PLM PALMITIC ACID × 24 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 8 LMT DODECYL-BETA-D-MALTOSIDE × 13 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å
9G6G Semi-active PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 3 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 LFA EICOSANE × 30 OEX CA-MN4-O5 CLUSTER × 1 LMT DODECYL-BETA-D-MALTOSIDE × 17 BCT BICARBONATE ION × 2 PLM PALMITIC ACID × 29 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 GOL GLYCEROL × 1 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 3 RRX (3R)-beta,beta-caroten-3-ol × 2 CA CALCIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å
9G6H active PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain L 1–37（37 aa）
Chain l 1–37（37 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 PLM PALMITIC ACID × 35 LFA EICOSANE × 24 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 GOL GLYCEROL × 2 LMT DODECYL-BETA-D-MALTOSIDE × 17 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 RRX (3R)-beta,beta-caroten-3-ol × 2 CA CALCIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å

63 other PDB entries and 68 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PSBL_THEEB
Isoform —
PDB entities 11
Chains and sequence ranges Author chain L； PDBConstruct 1–37； UniProt 1–37 Author chain l； PDBConstruct 1–37； UniProt 1–37

Photosystem II reaction center protein M

OrganismNot specified

UniProt Q8DHA7

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 40 PDB declaration: 40-meric（40） Consistent with protein copy count Chain M； UniProt 2–36 Chain m； UniProt 2–36 Non-standard monomer：Yes (specific site not provided by mmCIF) Photosystem II protein D1 1 × 2 （P0A444） Photosystem II CP47 reaction center protein × 2 （Q8DIQ1） Photosystem II CP43 reaction center protein × 2 （Q8DIF8） Photosystem II D2 protein × 2 （Q8CM25） Cytochrome b559 subunit alpha × 2 （Q8DIP0） Cytochrome b559 subunit beta × 2 （Q8DIN9） Photosystem II reaction center protein H × 2 （Q8DJ43） Photosystem II reaction center protein I × 2 （Q8DJZ6） Photosystem II reaction center protein J × 2 （P59087） Photosystem II reaction center protein K × 2 （Q9F1K9） Photosystem II reaction center protein L × 2 （Q8DIN8） Photosystem II manganese-stabilizing polypeptide × 2 （P0A431） Photosystem II reaction center protein T × 2 （Q8DIQ0） Photosystem II 12 kDa extrinsic protein × 2 （Q9F1L5） Cytochrome c-550 × 2 （P0A386） Photosystem II reaction center protein Ycf12 × 2 （Q8DJI1） Photosystem II reaction center X protein × 2 （Q9F1R6） Photosystem II reaction center protein Z × 2 （Q8DHJ2） Photosystem II protein Y × 2 （Q8DKM3） OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 UNL UNKNOWN LIGAND × 17 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION X-ray crystallization conditions：BATCH MODE；pH 7.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol. Resolution 3.00 Å R-free 0.303

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5KAF Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
11YO Single-conformation model re-refinement of 2F/S3-rich PSII intermediate structure at 2.09 Angstrom resolution Deposited 2026-03-18 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain M 1–36（36 aa）
Chain m 1–36（36 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 38 OEX CA-MN4-O5 CLUSTER × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 PHO PHEOPHYTIN A × 4 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.243
1S5L Architecture of the photosynthetic oxygen evolving center Deposited 2004-01-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain M 1–36（36 aa）
Not recorded FE FE (III) ION × 1 BCT BICARBONATE ION × 2 OEC OXYGEN EVOLVING SYSTEM × 1 CLA CHLOROPHYLL A × 36 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMT DODECYL-BETA-D-MALTOSIDE × 1 BCR BETA-CAROTENE × 7 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pseudo-batch hanging drop；pH 7.5；291 K；100mM HEPES, 100mM (NH4)2SO4, PEG 4000, C12E8, trimethyl lead acetate, pH 7.5, pseudo-batch hanging drop, temperature 291K
Resolution 3.50 Å R-free 0.342
1S5L Architecture of the photosynthetic oxygen evolving center Deposited 2004-01-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain m 1–36（36 aa）
Not recorded FE FE (III) ION × 1 BCT BICARBONATE ION × 2 OEC OXYGEN EVOLVING SYSTEM × 1 CLA CHLOROPHYLL A × 36 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMT DODECYL-BETA-D-MALTOSIDE × 1 BCR BETA-CAROTENE × 7 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pseudo-batch hanging drop；pH 7.5；291 K；100mM HEPES, 100mM (NH4)2SO4, PEG 4000, C12E8, trimethyl lead acetate, pH 7.5, pseudo-batch hanging drop, temperature 291K
Resolution 3.50 Å R-free 0.342
2AXT Crystal Structure of Photosystem II from Thermosynechococcus elongatus Deposited 2005-09-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 36 PDB declaration: 36-meric
Chain M 1–36（36 aa）
Chain m 1–36（36 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PQ9 5-[(2E,6E,10E,14E,18E,22E)-3,7,11,15,19,23,27-HEPTAMETHYLOCTACOSA-2,6,10,14,18,22,26-HEPTAENYL]-2,3-DIMETHYLBENZO-1,4-QUINONE × 4 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 22 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LMT DODECYL-BETA-D-MALTOSIDE × 6 MGE (1S)-2-(ALPHA-L-ALLOPYRANOSYLOXY)-1-[(TRIDECANOYLOXY)METHYL]ETHYL PALMITATE × 12 UNL UNKNOWN LIGAND × 34 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG 2000, PIPES, magnesium chloride, pH 7.0, batch, temperature 298K
Resolution 3.00 Å R-free 0.286
3KZI Crystal Structure of Monomeric Form of Cyanobacterial Photosystem II Deposited 2009-12-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain M 1–36（36 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 11 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 LMT DODECYL-BETA-D-MALTOSIDE × 7 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 BCT BICARBONATE ION × 1 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；291 K；PEG 400, PIPES, MES, CaCl2, pH 7, batch, temperature 291.0K
Resolution 3.60 Å R-free 0.308
4FBY fs X-ray diffraction of Photosystem II Deposited 2012-05-23 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain M 1–36（36 aa）
Chain e 1–36（36 aa）
Not recorded CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 OEC OXYGEN EVOLVING SYSTEM × 2 FE2 FE (II) ION × 2 BCR BETA-CAROTENE × 24 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 CL CHLORIDE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；298 K；4% PEG 2000, 5 mM CaCl2, 100 mM PIPES, pH 7.0, batch, temperature 298K
Resolution 6.56 Å R-free 0.385
4IXQ RT fs X-ray diffraction of Photosystem II, dark state Deposited 2013-01-27 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain M 1–36（36 aa）
Chain m 1–36（36 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 LMT DODECYL-BETA-D-MALTOSIDE × 14 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.70 Å R-free 0.314
4IXR RT fs X-ray diffraction of Photosystem II, first illuminated state Deposited 2013-01-27 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain M 1–36（36 aa）
Chain m 1–36（36 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 LMT DODECYL-BETA-D-MALTOSIDE × 14 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.90 Å R-free 0.313
4PBU Serial Time-resolved crystallography of Photosystem II using a femtosecond X-ray laser The S1 state Deposited 2014-04-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain M 1–34（34 aa）
Chain m 1–34（34 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 CA CALCIUM ION × 7 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 5.00 Å R-free 0.262
4PJ0 Structure of T.elongatus Photosystem II, rows of dimers crystal packing Deposited 2014-05-10 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain M 1–36（36 aa）
Chain m 1–36（36 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE FE (III) ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SO4 SULFATE ION × 10 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 29 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；pH 7.5；293 K；Batch crystallisation: 2 mM Chl a equivalent of PSIIcc mixed 1:1 with: 0.1 M TRIS (pH 7.5), 0.1 M (NH4)2SO4, 15 - 18 % PEG 5000 MME, post-crystallisation dehydration
Resolution 2.44 Å R-free 0.256
4RVY Serial Time resolved crystallography of Photosystem II using a femtosecond X-ray laser. The S state after two flashes (S3) Deposited 2014-11-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain M 1–34（34 aa）
Chain m 1–34（34 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 CA CALCIUM ION × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions Free interface diffusion；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000 , Free interface diffusion, temperature 283K, temperature 283.0K
Resolution 5.50 Å R-free 0.291
4TNH RT XFEL structure of Photosystem II in the dark state at 4.9 A resolution Deposited 2014-06-04 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain M 1–36（36 aa）
Chain m 1–36（36 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.90 Å R-free 0.292
4TNI RT XFEL structure of Photosystem II 500 ms after the third illumination at 4.6 A resolution Deposited 2014-06-04 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain M 1–36（36 aa）
Chain m 1–36（36 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 2 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.60 Å R-free 0.284
4TNJ RT XFEL structure of Photosystem II 500 ms after the 2nd illumination (2F) at 4.5 A resolution Deposited 2014-06-04 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain M 1–36（36 aa）
Chain m 1–36（36 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.50 Å R-free 0.284
4TNK RT XFEL structure of Photosystem II 250 microsec after the third illumination at 5.2 A resolution Deposited 2014-06-04 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain M 1–36（36 aa）
Chain m 1–36（36 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.20 Å R-free 0.289
4V62 Crystal Structure of cyanobacterial Photosystem II Deposited 2008-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain AM 1–36（36 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 3 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 13 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 7 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 CL CHLORIDE ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 7 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG2000, PIPES, magnesium chloride, pH7.0, batch, temperature 298K
Resolution 2.90 Å R-free 0.292
4V62 Crystal Structure of cyanobacterial Photosystem II Deposited 2008-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain BM 1–36（36 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 3 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 11 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 7 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 CL CHLORIDE ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 7 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG2000, PIPES, magnesium chloride, pH7.0, batch, temperature 298K
Resolution 2.90 Å R-free 0.292
4V82 Crystal structure of cyanobacterial Photosystem II in complex with terbutryn Deposited 2010-11-30 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain AM 1–36（36 aa）
Chain BM 1–36（36 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 70 MST 2-T-BUTYLAMINO-4-ETHYLAMINO-6-METHYLTHIO-S-TRIAZINE × 2 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 LMT DODECYL-BETA-D-MALTOSIDE × 16 DMS DIMETHYL SULFOXIDE × 8 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；Pipes, CaCl2, DMSO, PEG 2000, pH 7.0, batch, temperature 298K
Resolution 3.20 Å R-free 0.299
5E79 Macromolecular diffractive imaging using imperfect crystals Deposited 2015-10-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain M 1–34（34 aa）
Chain m 1–34（34 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 CA CALCIUM ION × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；283.18 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 3.50 Å R-free 0.331
5E7C Macromolecular diffractive imaging using imperfect crystals - Bragg data Deposited 2015-10-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain M 1–34（34 aa）
Chain m 1–34（34 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 FE2 FE (II) ION × 2 CA CALCIUM ION × 6 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 4.50 Å R-free 0.275
5KAI NH3-bound RT XFEL structure of Photosystem II 500 ms after the 2nd illumination (2F) at 2.8 A resolution Deposited 2016-06-01 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain M 2–36（35 aa）
Chain m 2–36（35 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 UNL UNKNOWN LIGAND × 23 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol
Resolution 2.80 Å R-free 0.300
5MX2 Photosystem II depleted of the Mn4CaO5 cluster at 2.55 A resolution Deposited 2017-01-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain M 1–36（36 aa）
Not recorded CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 11 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LFA EICOSANE × 19 FE FE (III) ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 4 BCT BICARBONATE ION × 1 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 7.5；291 K；equal volumes of buffer: 0.1 M Tris-HCl pH 7.5, 0.1 M (NH4)2SO4, 14-18 % PEG 5000 MME and protein: 2 mM chlorophyll equivalent of PSII in C12E8 detergent, post crystallisation treatment
Resolution 2.20 Å R-free 0.260
5MX2 Photosystem II depleted of the Mn4CaO5 cluster at 2.55 A resolution Deposited 2017-01-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain m 1–36（36 aa）
Not recorded CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 11 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LFA EICOSANE × 20 FE FE (III) ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 4 BCT BICARBONATE ION × 1 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 7.5；291 K；equal volumes of buffer: 0.1 M Tris-HCl pH 7.5, 0.1 M (NH4)2SO4, 14-18 % PEG 5000 MME and protein: 2 mM chlorophyll equivalent of PSII in C12E8 detergent, post crystallisation treatment
Resolution 2.20 Å R-free 0.260
5TIS Room temperature XFEL structure of the native, doubly-illuminated photosystem II complex Deposited 2016-10-03 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain M 1–36（36 aa）
Chain m 1–36（36 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 UNL UNKNOWN LIGAND × 33 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol
Resolution 2.25 Å R-free 0.231
5ZZN Crystal structure of photosystem II from an SQDG-deficient mutant of Thermosynechococcus elongatus Deposited 2018-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain M 1–34（34 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) OEX CA-MN4-O5 CLUSTER × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 5 UNL UNKNOWN LIGAND × 27 LMT DODECYL-BETA-D-MALTOSIDE × 9 DMS DIMETHYL SULFOXIDE × 48 CA CALCIUM ION × 3 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 HTG heptyl 1-thio-beta-D-glucopyranoside × 12 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 GOL GLYCEROL × 2 FE2 FE (II) ION × 1 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20MM NACL, 10MM CACL2, 40MM MGSO4, 20MM MES BUFFER, PH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPERATURE 285K
Resolution 2.10 Å R-free 0.197
5ZZN Crystal structure of photosystem II from an SQDG-deficient mutant of Thermosynechococcus elongatus Deposited 2018-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain m 1–34（34 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) OEX CA-MN4-O5 CLUSTER × 1 CL CHLORIDE ION × 3 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 5 UNL UNKNOWN LIGAND × 25 LMT DODECYL-BETA-D-MALTOSIDE × 10 DMS DIMETHYL SULFOXIDE × 47 CA CALCIUM ION × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 HTG heptyl 1-thio-beta-D-glucopyranoside × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 GOL GLYCEROL × 3 FE2 FE (II) ION × 1 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20MM NACL, 10MM CACL2, 40MM MGSO4, 20MM MES BUFFER, PH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPERATURE 285K
Resolution 2.10 Å R-free 0.197
6DHE RT XFEL structure of the dark-stable state of Photosystem II (0F, S1-rich) at 2.05 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain M 1–33（33 aa）
Chain m 1–33（33 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 8CT (6'R,11cis,11'cis,13cis,15cis)-4',5'-didehydro-5',6'-dihydro-beta,beta-carotene × 12 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 BCR BETA-CAROTENE × 10 UNL UNKNOWN LIGAND × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.05 Å R-free 0.241
6DHF RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.08 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain M 1–33（33 aa）
Chain m 1–33（33 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 UNL UNKNOWN LIGAND × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.247
6DHG RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.5 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain M 1–33（33 aa）
Chain m 1–33（33 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.50 Å R-free 0.246
6DHH RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.2 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain M 1–33（33 aa）
Chain m 1–33（33 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.20 Å R-free 0.264
6DHO RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.07 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain M 1–33（33 aa）
Chain m 1–33（33 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.07 Å R-free 0.247
6DHP RT XFEL structure of the three-flash state of Photosystem II (3F, S0-rich) at 2.04 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain M 1–33（33 aa）
Chain m 1–33（33 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 UNL UNKNOWN LIGAND × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.04 Å R-free 0.248
6W1O RT XFEL structure of the dark-stable state of Photosystem II (0F, S1-rich) at 2.08 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain M 2–36（35 aa）
Chain m 2–36（35 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.240
6W1P RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.26 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain M 2–36（35 aa）
Chain m 2–36（35 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.26 Å R-free 0.241
6W1Q RT XFEL structure of Photosystem II 50 microseconds after the second illumination at 2.27 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain M 2–36（35 aa）
Chain m 2–36（35 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 BCR BETA-CAROTENE × 22 STE STEARIC ACID × 35 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 HEC HEME C × 4 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.27 Å R-free 0.249
6W1R RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.23 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain M 2–36（35 aa）
Chain m 2–36（35 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.23 Å R-free 0.235
6W1T RT XFEL structure of Photosystem II 250 microseconds after the second illumination at 2.01 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain M 2–36（35 aa）
Chain m 2–36（35 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.01 Å R-free 0.228
6W1U RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.09 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain M 2–36（35 aa）
Chain m 2–36（35 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
6W1V RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain M 2–36（35 aa）
Chain m 2–36（35 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 STE STEARIC ACID × 38 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 PHO PHEOPHYTIN A × 4 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.241
7NHO Structure of PSII-M Deposited 2021-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain M 1–36（36 aa）
Not recorded FE FE (III) ION × 1 MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 BCT BICARBONATE ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.66 Å
7NHP Structure of PSII-I (PSII with Psb27, Psb28, and Psb34) Deposited 2021-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain M 1–36（36 aa）
Not recorded MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 FE FE (III) ION × 1 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.72 Å
7NHQ Structure of PSII-I prime (PSII with Psb28, and Psb34) Deposited 2021-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 17 PDB declaration: heptadecameric
Chain M 1–36（36 aa）
Not recorded FE FE (III) ION × 1 MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.68 Å
7RF1 RT XFEL structure of Photosystem II averaged across all S-states at 1.89 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain M 2–36（35 aa）
Chain m 2–36（35 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 STE STEARIC ACID × 55 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 1.89 Å R-free 0.214
7RF2 RT XFEL structure of dark-stable state of Photosystem II (0F, S1 rich) at 2.08 Angstrom Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain M 2–36（35 aa）
Chain m 2–36（35 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 33 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.238
7RF3 RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.26 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain M 2–36（35 aa）
Chain m 2–36（35 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.26 Å R-free 0.238
7RF4 RT XFEL structure of Photosystem II 50 microseconds after the second illumination at 2.27 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain M 2–36（35 aa）
Chain m 2–36（35 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 BCR BETA-CAROTENE × 22 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.27 Å R-free 0.244
7RF5 RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.23 Angstrom resolution Deposited 2021-07-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain M 1–36（36 aa）
Chain m 1–36（36 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.23 Å R-free 0.233
7RF6 RT XFEL structure of Photosystem II 250 microseconds after the second illumination at 2.01 Angstrom resolution Deposited 2021-07-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain M 1–36（36 aa）
Chain m 1–36（36 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.01 Å R-free 0.227
7RF7 RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.09 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain M 2–36（35 aa）
Chain m 2–36（35 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
7RF8 RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain M 2–36（35 aa）
Chain m 2–36（35 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
7YQ2 Crystal structure of photosystem II expressing psbA2 gene only Deposited 2022-08-05 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 39 PDB declaration: 39-meric
Chain M 1–36（36 aa）
Chain m 1–36（36 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 UNL UNKNOWN LIGAND × 77 LMT DODECYL-BETA-D-MALTOSIDE × 19 GOL GLYCEROL × 10 BCT BICARBONATE ION × 3 DMS DIMETHYL SULFOXIDE × 116 CA CALCIUM ION × 7 HTG heptyl 1-thio-beta-D-glucopyranoside × 31 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 9 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 MG MAGNESIUM ION × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20mM NaCl, 10mM CaCl2, 40mM MgSO4, 20mM Mes BUFFER, pH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPARATURE 285K
Resolution 1.90 Å R-free 0.184
7YQ7 Crystal structure of photosystem II expressing psbA3 gene only Deposited 2022-08-05 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 39 PDB declaration: 39-meric
Chain M 1–36（36 aa）
Chain m 1–36（36 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 UNL UNKNOWN LIGAND × 82 GOL GLYCEROL × 11 LMT DODECYL-BETA-D-MALTOSIDE × 18 BCT BICARBONATE ION × 4 DMS DIMETHYL SULFOXIDE × 71 CA CALCIUM ION × 5 HTG heptyl 1-thio-beta-D-glucopyranoside × 18 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 MG MAGNESIUM ION × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20mM NaCl, 10mM CaCl2, 40mM MgSO4, 20mM MES BUFFER pH 6.0, MICRO-BATCH METHOD UNDER OIL, TMPERATURE 285K
Resolution 1.90 Å R-free 0.177
8EZ5 RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2022-10-31 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain M 1–36（36 aa）
Chain m 1–36（36 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.234
8F4C RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.00 Angstrom resolution Deposited 2022-11-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain M 1–36（36 aa）
Chain m 1–36（36 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.224
8F4D RT XFEL structure of Photosystem II 50 microseconds after the third illumination at 2.15 Angstrom resolution Deposited 2022-11-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain M 1–36（36 aa）
Chain m 1–36（36 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.15 Å R-free 0.243
8F4E RT XFEL structure of Photosystem II 250 microseconds after the third illumination at 2.09 Angstrom resolution Deposited 2022-11-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain M 1–36（36 aa）
Chain m 1–36（36 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.233
8F4F RT XFEL structure of Photosystem II 500 microseconds after the third illumination at 2.03 Angstrom resolution Deposited 2022-11-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain M 1–36（36 aa）
Chain m 1–36（36 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.03 Å R-free 0.248
8F4G RT XFEL structure of Photosystem II 730 microseconds after the third illumination at 2.03 Angstrom resolution Deposited 2022-11-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain M 1–36（36 aa）
Chain m 1–36（36 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.03 Å R-free 0.223
8F4H RT XFEL structure of Photosystem II 1200 microseconds after the third illumination at 2.10 Angstrom resolution Deposited 2022-11-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain M 1–36（36 aa）
Chain m 1–36（36 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.10 Å R-free 0.232
8F4I RT XFEL structure of Photosystem II 2000 microseconds after the third illumination at 2.00 Angstrom resolution Deposited 2022-11-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain M 1–36（36 aa）
Chain m 1–36（36 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.232
8F4J RT XFEL structure of Photosystem II 4000 microseconds after the third illumination at 2.00 Angstrom resolution Deposited 2022-11-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain M 1–36（36 aa）
Chain m 1–36（36 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.226
8F4K RT XFEL structure of the three-flash state of Photosystem II (3F, S0-rich) at 2.16 Angstrom resolution Deposited 2022-11-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain M 1–36（36 aa）
Chain m 1–36（36 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.16 Å R-free 0.241
9EVX cryoEM structure of Photosystem II averaged across S2-S3 states at 1.71 Angstrom resolution Deposited 2024-04-02 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain M 1–36（36 aa）
Chain m 1–36（36 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 STE STEARIC ACID × 42 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 PHO PHEOPHYTIN A × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；100mM MES pH6.5, 5mM CaCl2, 5% glycerol, 0.03% DDM
cryo-EM vitrification conditions Cryogen ETHANE；The sample-on-grid was illuminated with one and two flashes of light
Resolution 1.71 Å
9G6F Inactive PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain M 1–36（36 aa）
Chain m 1–36（36 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LFA EICOSANE × 33 PLM PALMITIC ACID × 24 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 8 LMT DODECYL-BETA-D-MALTOSIDE × 13 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å
9G6G Semi-active PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric
Chain M 1–36（36 aa）
Chain m 1–36（36 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 3 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 LFA EICOSANE × 30 OEX CA-MN4-O5 CLUSTER × 1 LMT DODECYL-BETA-D-MALTOSIDE × 17 BCT BICARBONATE ION × 2 PLM PALMITIC ACID × 29 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 GOL GLYCEROL × 1 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 3 RRX (3R)-beta,beta-caroten-3-ol × 2 CA CALCIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å
9G6H active PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain M 1–36（36 aa）
Chain m 1–36（36 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 PLM PALMITIC ACID × 35 LFA EICOSANE × 24 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 GOL GLYCEROL × 2 LMT DODECYL-BETA-D-MALTOSIDE × 17 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 RRX (3R)-beta,beta-caroten-3-ol × 2 CA CALCIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å

62 other PDB entries and 66 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PSBM_THEEB
Isoform —
PDB entities 12
Chains and sequence ranges Author chain M； PDBConstruct 2–36； UniProt 2–36 Author chain m； PDBConstruct 2–36； UniProt 2–36

Photosystem II manganese-stabilizing polypeptide

OrganismNot specified

UniProt P0A431

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 40 PDB declaration: 40-meric（40） Consistent with protein copy count Chain O； UniProt 1–272 Chain o； UniProt 1–272 Not recorded Photosystem II protein D1 1 × 2 （P0A444） Photosystem II CP47 reaction center protein × 2 （Q8DIQ1） Photosystem II CP43 reaction center protein × 2 （Q8DIF8） Photosystem II D2 protein × 2 （Q8CM25） Cytochrome b559 subunit alpha × 2 （Q8DIP0） Cytochrome b559 subunit beta × 2 （Q8DIN9） Photosystem II reaction center protein H × 2 （Q8DJ43） Photosystem II reaction center protein I × 2 （Q8DJZ6） Photosystem II reaction center protein J × 2 （P59087） Photosystem II reaction center protein K × 2 （Q9F1K9） Photosystem II reaction center protein L × 2 （Q8DIN8） Photosystem II reaction center protein M × 2 （Q8DHA7） Photosystem II reaction center protein T × 2 （Q8DIQ0） Photosystem II 12 kDa extrinsic protein × 2 （Q9F1L5） Cytochrome c-550 × 2 （P0A386） Photosystem II reaction center protein Ycf12 × 2 （Q8DJI1） Photosystem II reaction center X protein × 2 （Q9F1R6） Photosystem II reaction center protein Z × 2 （Q8DHJ2） Photosystem II protein Y × 2 （Q8DKM3） OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 UNL UNKNOWN LIGAND × 17 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION X-ray crystallization conditions：BATCH MODE；pH 7.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol. Resolution 3.00 Å R-free 0.303

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5KAF Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
11YO Single-conformation model re-refinement of 2F/S3-rich PSII intermediate structure at 2.09 Angstrom resolution Deposited 2026-03-18 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain O 1–272（272 aa）
Chain o 1–272（272 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 38 OEX CA-MN4-O5 CLUSTER × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 PHO PHEOPHYTIN A × 4 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.243
1S5L Architecture of the photosynthetic oxygen evolving center Deposited 2004-01-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain O 27–272（246 aa）
Not recorded FE FE (III) ION × 1 BCT BICARBONATE ION × 2 OEC OXYGEN EVOLVING SYSTEM × 1 CLA CHLOROPHYLL A × 36 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMT DODECYL-BETA-D-MALTOSIDE × 1 BCR BETA-CAROTENE × 7 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pseudo-batch hanging drop；pH 7.5；291 K；100mM HEPES, 100mM (NH4)2SO4, PEG 4000, C12E8, trimethyl lead acetate, pH 7.5, pseudo-batch hanging drop, temperature 291K
Resolution 3.50 Å R-free 0.342
1S5L Architecture of the photosynthetic oxygen evolving center Deposited 2004-01-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain o 27–272（246 aa）
Not recorded FE FE (III) ION × 1 BCT BICARBONATE ION × 2 OEC OXYGEN EVOLVING SYSTEM × 1 CLA CHLOROPHYLL A × 36 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMT DODECYL-BETA-D-MALTOSIDE × 1 BCR BETA-CAROTENE × 7 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pseudo-batch hanging drop；pH 7.5；291 K；100mM HEPES, 100mM (NH4)2SO4, PEG 4000, C12E8, trimethyl lead acetate, pH 7.5, pseudo-batch hanging drop, temperature 291K
Resolution 3.50 Å R-free 0.342
2AXT Crystal Structure of Photosystem II from Thermosynechococcus elongatus Deposited 2005-09-06 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 36 PDB declaration: 36-meric
Chain O 26–272（247 aa）
Chain o 26–272（247 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PQ9 5-[(2E,6E,10E,14E,18E,22E)-3,7,11,15,19,23,27-HEPTAMETHYLOCTACOSA-2,6,10,14,18,22,26-HEPTAENYL]-2,3-DIMETHYLBENZO-1,4-QUINONE × 4 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 22 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LMT DODECYL-BETA-D-MALTOSIDE × 6 MGE (1S)-2-(ALPHA-L-ALLOPYRANOSYLOXY)-1-[(TRIDECANOYLOXY)METHYL]ETHYL PALMITATE × 12 UNL UNKNOWN LIGAND × 34 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG 2000, PIPES, magnesium chloride, pH 7.0, batch, temperature 298K
Resolution 3.00 Å R-free 0.286
3KZI Crystal Structure of Monomeric Form of Cyanobacterial Photosystem II Deposited 2009-12-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain O 27–272（246 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 11 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 LMT DODECYL-BETA-D-MALTOSIDE × 7 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 BCT BICARBONATE ION × 1 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；291 K；PEG 400, PIPES, MES, CaCl2, pH 7, batch, temperature 291.0K
Resolution 3.60 Å R-free 0.308
4FBY fs X-ray diffraction of Photosystem II Deposited 2012-05-23 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain O 27–272（246 aa）
Chain f 27–272（246 aa）
Not recorded CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 OEC OXYGEN EVOLVING SYSTEM × 2 FE2 FE (II) ION × 2 BCR BETA-CAROTENE × 24 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 CL CHLORIDE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；298 K；4% PEG 2000, 5 mM CaCl2, 100 mM PIPES, pH 7.0, batch, temperature 298K
Resolution 6.56 Å R-free 0.385
4IXQ RT fs X-ray diffraction of Photosystem II, dark state Deposited 2013-01-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain O 1–272（272 aa）
Chain o 1–272（272 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 LMT DODECYL-BETA-D-MALTOSIDE × 14 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.70 Å R-free 0.314
4IXR RT fs X-ray diffraction of Photosystem II, first illuminated state Deposited 2013-01-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain O 1–272（272 aa）
Chain o 1–272（272 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 LMT DODECYL-BETA-D-MALTOSIDE × 14 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.90 Å R-free 0.313
4PBU Serial Time-resolved crystallography of Photosystem II using a femtosecond X-ray laser The S1 state Deposited 2014-04-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain O 30–272（243 aa）
Chain o 30–272（243 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 CA CALCIUM ION × 7 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 5.00 Å R-free 0.262
4PJ0 Structure of T.elongatus Photosystem II, rows of dimers crystal packing Deposited 2014-05-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain O 1–272（272 aa）
Chain o 1–272（272 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE FE (III) ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SO4 SULFATE ION × 10 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 29 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；pH 7.5；293 K；Batch crystallisation: 2 mM Chl a equivalent of PSIIcc mixed 1:1 with: 0.1 M TRIS (pH 7.5), 0.1 M (NH4)2SO4, 15 - 18 % PEG 5000 MME, post-crystallisation dehydration
Resolution 2.44 Å R-free 0.256
4RVY Serial Time resolved crystallography of Photosystem II using a femtosecond X-ray laser. The S state after two flashes (S3) Deposited 2014-11-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain O 30–272（243 aa）
Chain o 30–272（243 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 CA CALCIUM ION × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions Free interface diffusion；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000 , Free interface diffusion, temperature 283K, temperature 283.0K
Resolution 5.50 Å R-free 0.291
4TNH RT XFEL structure of Photosystem II in the dark state at 4.9 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain O 1–272（272 aa）
Chain o 1–272（272 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.90 Å R-free 0.292
4TNI RT XFEL structure of Photosystem II 500 ms after the third illumination at 4.6 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain O 1–272（272 aa）
Chain o 1–272（272 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 2 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.60 Å R-free 0.284
4TNJ RT XFEL structure of Photosystem II 500 ms after the 2nd illumination (2F) at 4.5 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain O 1–272（272 aa）
Chain o 1–272（272 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.50 Å R-free 0.284
4TNK RT XFEL structure of Photosystem II 250 microsec after the third illumination at 5.2 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain O 1–272（272 aa）
Chain o 1–272（272 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.20 Å R-free 0.289
4V62 Crystal Structure of cyanobacterial Photosystem II Deposited 2008-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain AO 26–272（247 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 3 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 13 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 7 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 CL CHLORIDE ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 7 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG2000, PIPES, magnesium chloride, pH7.0, batch, temperature 298K
Resolution 2.90 Å R-free 0.292
4V62 Crystal Structure of cyanobacterial Photosystem II Deposited 2008-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain BO 26–272（247 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 3 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 11 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 7 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 CL CHLORIDE ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 7 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG2000, PIPES, magnesium chloride, pH7.0, batch, temperature 298K
Resolution 2.90 Å R-free 0.292
4V82 Crystal structure of cyanobacterial Photosystem II in complex with terbutryn Deposited 2010-11-30 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain AO 26–272（247 aa）
Chain BO 26–272（247 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 70 MST 2-T-BUTYLAMINO-4-ETHYLAMINO-6-METHYLTHIO-S-TRIAZINE × 2 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 LMT DODECYL-BETA-D-MALTOSIDE × 16 DMS DIMETHYL SULFOXIDE × 8 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；Pipes, CaCl2, DMSO, PEG 2000, pH 7.0, batch, temperature 298K
Resolution 3.20 Å R-free 0.299
5E79 Macromolecular diffractive imaging using imperfect crystals Deposited 2015-10-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain O 30–272（243 aa）
Chain o 30–272（243 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 CA CALCIUM ION × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；283.18 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 3.50 Å R-free 0.331
5E7C Macromolecular diffractive imaging using imperfect crystals - Bragg data Deposited 2015-10-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain O 30–272（243 aa）
Chain o 30–272（243 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 FE2 FE (II) ION × 2 CA CALCIUM ION × 6 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 4.50 Å R-free 0.275
5H2F Crystal structure of the PsbM-deletion mutant of photosystem II Deposited 2016-10-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain O 30–272（243 aa） Fragment：UNP RESIDUES 30-272
Not recorded OEX CA-MN4-O5 CLUSTER × 1 FE2 FE (II) ION × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 3 LMT DODECYL-BETA-D-MALTOSIDE × 6 UNL UNKNOWN LIGAND × 13 DMS DIMETHYL SULFOXIDE × 67 BCT BICARBONATE ION × 1 CA CALCIUM ION × 2 HTG heptyl 1-thio-beta-D-glucopyranoside × 9 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6.1；285 K；PEG1450, sodium chloride, calcium chloride, magnesium sulfate
Resolution 2.20 Å R-free 0.226
5H2F Crystal structure of the PsbM-deletion mutant of photosystem II Deposited 2016-10-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain o 30–272（243 aa） Fragment：UNP RESIDUES 30-272
Not recorded OEX CA-MN4-O5 CLUSTER × 1 FE2 FE (II) ION × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 5 LMT DODECYL-BETA-D-MALTOSIDE × 5 UNL UNKNOWN LIGAND × 14 DMS DIMETHYL SULFOXIDE × 66 BCT BICARBONATE ION × 1 CA CALCIUM ION × 3 HTG heptyl 1-thio-beta-D-glucopyranoside × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6.1；285 K；PEG1450, sodium chloride, calcium chloride, magnesium sulfate
Resolution 2.20 Å R-free 0.226
5KAI NH3-bound RT XFEL structure of Photosystem II 500 ms after the 2nd illumination (2F) at 2.8 A resolution Deposited 2016-06-01 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain O 1–272（272 aa）
Chain o 1–272（272 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 UNL UNKNOWN LIGAND × 23 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol
Resolution 2.80 Å R-free 0.300
5MX2 Photosystem II depleted of the Mn4CaO5 cluster at 2.55 A resolution Deposited 2017-01-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain O 1–272（272 aa）
Not recorded CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 11 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LFA EICOSANE × 19 FE FE (III) ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 4 BCT BICARBONATE ION × 1 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 7.5；291 K；equal volumes of buffer: 0.1 M Tris-HCl pH 7.5, 0.1 M (NH4)2SO4, 14-18 % PEG 5000 MME and protein: 2 mM chlorophyll equivalent of PSII in C12E8 detergent, post crystallisation treatment
Resolution 2.20 Å R-free 0.260
5MX2 Photosystem II depleted of the Mn4CaO5 cluster at 2.55 A resolution Deposited 2017-01-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain o 1–272（272 aa）
Not recorded CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 11 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LFA EICOSANE × 20 FE FE (III) ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 4 BCT BICARBONATE ION × 1 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 7.5；291 K；equal volumes of buffer: 0.1 M Tris-HCl pH 7.5, 0.1 M (NH4)2SO4, 14-18 % PEG 5000 MME and protein: 2 mM chlorophyll equivalent of PSII in C12E8 detergent, post crystallisation treatment
Resolution 2.20 Å R-free 0.260
5TIS Room temperature XFEL structure of the native, doubly-illuminated photosystem II complex Deposited 2016-10-03 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain O 1–272（272 aa）
Chain o 1–272（272 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 UNL UNKNOWN LIGAND × 33 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol
Resolution 2.25 Å R-free 0.231
5ZZN Crystal structure of photosystem II from an SQDG-deficient mutant of Thermosynechococcus elongatus Deposited 2018-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain O 30–272（243 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 5 UNL UNKNOWN LIGAND × 27 LMT DODECYL-BETA-D-MALTOSIDE × 9 DMS DIMETHYL SULFOXIDE × 48 CA CALCIUM ION × 3 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 HTG heptyl 1-thio-beta-D-glucopyranoside × 12 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 GOL GLYCEROL × 2 FE2 FE (II) ION × 1 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20MM NACL, 10MM CACL2, 40MM MGSO4, 20MM MES BUFFER, PH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPERATURE 285K
Resolution 2.10 Å R-free 0.197
5ZZN Crystal structure of photosystem II from an SQDG-deficient mutant of Thermosynechococcus elongatus Deposited 2018-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain o 30–272（243 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 1 CL CHLORIDE ION × 3 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 5 UNL UNKNOWN LIGAND × 25 LMT DODECYL-BETA-D-MALTOSIDE × 10 DMS DIMETHYL SULFOXIDE × 47 CA CALCIUM ION × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 HTG heptyl 1-thio-beta-D-glucopyranoside × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 GOL GLYCEROL × 3 FE2 FE (II) ION × 1 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20MM NACL, 10MM CACL2, 40MM MGSO4, 20MM MES BUFFER, PH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPERATURE 285K
Resolution 2.10 Å R-free 0.197
6DHE RT XFEL structure of the dark-stable state of Photosystem II (0F, S1-rich) at 2.05 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain O 29–272（244 aa）
Chain o 29–272（244 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 8CT (6'R,11cis,11'cis,13cis,15cis)-4',5'-didehydro-5',6'-dihydro-beta,beta-carotene × 12 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 BCR BETA-CAROTENE × 10 UNL UNKNOWN LIGAND × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.05 Å R-free 0.241
6DHF RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.08 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain O 29–272（244 aa）
Chain o 29–272（244 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 UNL UNKNOWN LIGAND × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.247
6DHG RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.5 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain O 29–272（244 aa）
Chain o 29–272（244 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.50 Å R-free 0.246
6DHH RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.2 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain O 29–272（244 aa）
Chain o 29–272（244 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.20 Å R-free 0.264
6DHO RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.07 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain O 29–272（244 aa）
Chain o 29–272（244 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.07 Å R-free 0.247
6DHP RT XFEL structure of the three-flash state of Photosystem II (3F, S0-rich) at 2.04 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain O 29–272（244 aa）
Chain o 29–272（244 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 UNL UNKNOWN LIGAND × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.04 Å R-free 0.248
6W1O RT XFEL structure of the dark-stable state of Photosystem II (0F, S1-rich) at 2.08 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain O 1–272（272 aa）
Chain o 1–272（272 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.240
6W1P RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.26 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain O 1–272（272 aa）
Chain o 1–272（272 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.26 Å R-free 0.241
6W1Q RT XFEL structure of Photosystem II 50 microseconds after the second illumination at 2.27 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain O 1–272（272 aa）
Chain o 1–272（272 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 BCR BETA-CAROTENE × 22 STE STEARIC ACID × 35 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 HEC HEME C × 4 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.27 Å R-free 0.249
6W1R RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.23 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain O 1–272（272 aa）
Chain o 1–272（272 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.23 Å R-free 0.235
6W1T RT XFEL structure of Photosystem II 250 microseconds after the second illumination at 2.01 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain O 1–272（272 aa）
Chain o 1–272（272 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.01 Å R-free 0.228
6W1U RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.09 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain O 1–272（272 aa）
Chain o 1–272（272 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
6W1V RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain O 1–272（272 aa）
Chain o 1–272（272 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 STE STEARIC ACID × 38 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 PHO PHEOPHYTIN A × 4 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.241
7RF1 RT XFEL structure of Photosystem II averaged across all S-states at 1.89 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain O 1–272（272 aa）
Chain o 1–272（272 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 STE STEARIC ACID × 55 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 1.89 Å R-free 0.214
7RF2 RT XFEL structure of dark-stable state of Photosystem II (0F, S1 rich) at 2.08 Angstrom Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain O 1–272（272 aa）
Chain o 1–272（272 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 33 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.238
7RF3 RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.26 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain O 1–272（272 aa）
Chain o 1–272（272 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.26 Å R-free 0.238
7RF4 RT XFEL structure of Photosystem II 50 microseconds after the second illumination at 2.27 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain O 1–272（272 aa）
Chain o 1–272（272 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 BCR BETA-CAROTENE × 22 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.27 Å R-free 0.244
7RF5 RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.23 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain O 1–272（272 aa）
Chain o 1–272（272 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.23 Å R-free 0.233
7RF6 RT XFEL structure of Photosystem II 250 microseconds after the second illumination at 2.01 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain O 1–272（272 aa）
Chain o 1–272（272 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.01 Å R-free 0.227
7RF7 RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.09 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain O 1–272（272 aa）
Chain o 1–272（272 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
7RF8 RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain O 1–272（272 aa）
Chain o 1–272（272 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
7YQ2 Crystal structure of photosystem II expressing psbA2 gene only Deposited 2022-08-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 39 PDB declaration: 39-meric
Chain O 1–272（272 aa）
Chain o 1–272（272 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 UNL UNKNOWN LIGAND × 77 LMT DODECYL-BETA-D-MALTOSIDE × 19 GOL GLYCEROL × 10 BCT BICARBONATE ION × 3 DMS DIMETHYL SULFOXIDE × 116 CA CALCIUM ION × 7 HTG heptyl 1-thio-beta-D-glucopyranoside × 31 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 9 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 MG MAGNESIUM ION × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20mM NaCl, 10mM CaCl2, 40mM MgSO4, 20mM Mes BUFFER, pH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPARATURE 285K
Resolution 1.90 Å R-free 0.184
7YQ7 Crystal structure of photosystem II expressing psbA3 gene only Deposited 2022-08-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 39 PDB declaration: 39-meric
Chain O 1–272（272 aa）
Chain o 1–272（272 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 UNL UNKNOWN LIGAND × 82 GOL GLYCEROL × 11 LMT DODECYL-BETA-D-MALTOSIDE × 18 BCT BICARBONATE ION × 4 DMS DIMETHYL SULFOXIDE × 71 CA CALCIUM ION × 5 HTG heptyl 1-thio-beta-D-glucopyranoside × 18 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 MG MAGNESIUM ION × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20mM NaCl, 10mM CaCl2, 40mM MgSO4, 20mM MES BUFFER pH 6.0, MICRO-BATCH METHOD UNDER OIL, TMPERATURE 285K
Resolution 1.90 Å R-free 0.177
8EZ5 RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2022-10-31 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain O 1–272（272 aa）
Chain o 1–272（272 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.234
8F4C RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain O 1–272（272 aa）
Chain o 1–272（272 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.224
8F4D RT XFEL structure of Photosystem II 50 microseconds after the third illumination at 2.15 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain O 1–272（272 aa）
Chain o 1–272（272 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.15 Å R-free 0.243
8F4E RT XFEL structure of Photosystem II 250 microseconds after the third illumination at 2.09 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain O 1–272（272 aa）
Chain o 1–272（272 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.233
8F4F RT XFEL structure of Photosystem II 500 microseconds after the third illumination at 2.03 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain O 1–272（272 aa）
Chain o 1–272（272 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.03 Å R-free 0.248
8F4G RT XFEL structure of Photosystem II 730 microseconds after the third illumination at 2.03 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain O 1–272（272 aa）
Chain o 1–272（272 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.03 Å R-free 0.223
8F4H RT XFEL structure of Photosystem II 1200 microseconds after the third illumination at 2.10 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain O 1–272（272 aa）
Chain o 1–272（272 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.10 Å R-free 0.232
8F4I RT XFEL structure of Photosystem II 2000 microseconds after the third illumination at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain O 1–272（272 aa）
Chain o 1–272（272 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.232
8F4J RT XFEL structure of Photosystem II 4000 microseconds after the third illumination at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain O 1–272（272 aa）
Chain o 1–272（272 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.226
8F4K RT XFEL structure of the three-flash state of Photosystem II (3F, S0-rich) at 2.16 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain O 1–272（272 aa）
Chain o 1–272（272 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.16 Å R-free 0.241
9EVX cryoEM structure of Photosystem II averaged across S2-S3 states at 1.71 Angstrom resolution Deposited 2024-04-02 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain O 1–272（272 aa）
Chain o 1–272（272 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 STE STEARIC ACID × 42 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 PHO PHEOPHYTIN A × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；100mM MES pH6.5, 5mM CaCl2, 5% glycerol, 0.03% DDM
cryo-EM vitrification conditions Cryogen ETHANE；The sample-on-grid was illuminated with one and two flashes of light
Resolution 1.71 Å
9G6G Semi-active PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric
Chain O 1–272（272 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 3 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 LFA EICOSANE × 30 OEX CA-MN4-O5 CLUSTER × 1 LMT DODECYL-BETA-D-MALTOSIDE × 17 BCT BICARBONATE ION × 2 PLM PALMITIC ACID × 29 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 GOL GLYCEROL × 1 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 3 RRX (3R)-beta,beta-caroten-3-ol × 2 CA CALCIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å
9G6H active PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain O 1–272（272 aa）
Chain o 1–272（272 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 PLM PALMITIC ACID × 35 LFA EICOSANE × 24 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 GOL GLYCEROL × 2 LMT DODECYL-BETA-D-MALTOSIDE × 17 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 RRX (3R)-beta,beta-caroten-3-ol × 2 CA CALCIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å

59 other PDB entries and 64 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PSBO_THEEB
Isoform —
PDB entities 13
Chains and sequence ranges Author chain O； PDBConstruct 1–272； UniProt 1–272 Author chain o； PDBConstruct 1–272； UniProt 1–272

Photosystem II reaction center protein T

OrganismNot specified

UniProt Q8DIQ0

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 40 PDB declaration: 40-meric（40） Consistent with protein copy count Chain T； UniProt 2–32 Chain t； UniProt 2–32 Non-standard monomer：Yes (specific site not provided by mmCIF) Photosystem II protein D1 1 × 2 （P0A444） Photosystem II CP47 reaction center protein × 2 （Q8DIQ1） Photosystem II CP43 reaction center protein × 2 （Q8DIF8） Photosystem II D2 protein × 2 （Q8CM25） Cytochrome b559 subunit alpha × 2 （Q8DIP0） Cytochrome b559 subunit beta × 2 （Q8DIN9） Photosystem II reaction center protein H × 2 （Q8DJ43） Photosystem II reaction center protein I × 2 （Q8DJZ6） Photosystem II reaction center protein J × 2 （P59087） Photosystem II reaction center protein K × 2 （Q9F1K9） Photosystem II reaction center protein L × 2 （Q8DIN8） Photosystem II reaction center protein M × 2 （Q8DHA7） Photosystem II manganese-stabilizing polypeptide × 2 （P0A431） Photosystem II 12 kDa extrinsic protein × 2 （Q9F1L5） Cytochrome c-550 × 2 （P0A386） Photosystem II reaction center protein Ycf12 × 2 （Q8DJI1） Photosystem II reaction center X protein × 2 （Q9F1R6） Photosystem II reaction center protein Z × 2 （Q8DHJ2） Photosystem II protein Y × 2 （Q8DKM3） OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 UNL UNKNOWN LIGAND × 17 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION X-ray crystallization conditions：BATCH MODE；pH 7.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol. Resolution 3.00 Å R-free 0.303

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5KAF Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
11YO Single-conformation model re-refinement of 2F/S3-rich PSII intermediate structure at 2.09 Angstrom resolution Deposited 2026-03-18 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain T 1–32（32 aa）
Chain t 1–32（32 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 38 OEX CA-MN4-O5 CLUSTER × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 PHO PHEOPHYTIN A × 4 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.243
1S5L Architecture of the photosynthetic oxygen evolving center Deposited 2004-01-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain T 1–32（32 aa）
Not recorded FE FE (III) ION × 1 BCT BICARBONATE ION × 2 OEC OXYGEN EVOLVING SYSTEM × 1 CLA CHLOROPHYLL A × 36 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMT DODECYL-BETA-D-MALTOSIDE × 1 BCR BETA-CAROTENE × 7 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pseudo-batch hanging drop；pH 7.5；291 K；100mM HEPES, 100mM (NH4)2SO4, PEG 4000, C12E8, trimethyl lead acetate, pH 7.5, pseudo-batch hanging drop, temperature 291K
Resolution 3.50 Å R-free 0.342
1S5L Architecture of the photosynthetic oxygen evolving center Deposited 2004-01-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain t 1–32（32 aa）
Not recorded FE FE (III) ION × 1 BCT BICARBONATE ION × 2 OEC OXYGEN EVOLVING SYSTEM × 1 CLA CHLOROPHYLL A × 36 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMT DODECYL-BETA-D-MALTOSIDE × 1 BCR BETA-CAROTENE × 7 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pseudo-batch hanging drop；pH 7.5；291 K；100mM HEPES, 100mM (NH4)2SO4, PEG 4000, C12E8, trimethyl lead acetate, pH 7.5, pseudo-batch hanging drop, temperature 291K
Resolution 3.50 Å R-free 0.342
2AXT Crystal Structure of Photosystem II from Thermosynechococcus elongatus Deposited 2005-09-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 36 PDB declaration: 36-meric
Chain T 1–32（32 aa）
Chain t 1–32（32 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PQ9 5-[(2E,6E,10E,14E,18E,22E)-3,7,11,15,19,23,27-HEPTAMETHYLOCTACOSA-2,6,10,14,18,22,26-HEPTAENYL]-2,3-DIMETHYLBENZO-1,4-QUINONE × 4 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 22 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LMT DODECYL-BETA-D-MALTOSIDE × 6 MGE (1S)-2-(ALPHA-L-ALLOPYRANOSYLOXY)-1-[(TRIDECANOYLOXY)METHYL]ETHYL PALMITATE × 12 UNL UNKNOWN LIGAND × 34 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG 2000, PIPES, magnesium chloride, pH 7.0, batch, temperature 298K
Resolution 3.00 Å R-free 0.286
3KZI Crystal Structure of Monomeric Form of Cyanobacterial Photosystem II Deposited 2009-12-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain T 1–32（32 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 11 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 LMT DODECYL-BETA-D-MALTOSIDE × 7 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 BCT BICARBONATE ION × 1 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；291 K；PEG 400, PIPES, MES, CaCl2, pH 7, batch, temperature 291.0K
Resolution 3.60 Å R-free 0.308
4FBY fs X-ray diffraction of Photosystem II Deposited 2012-05-23 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain T 1–32（32 aa）
Chain g 1–32（32 aa）
Not recorded CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 OEC OXYGEN EVOLVING SYSTEM × 2 FE2 FE (II) ION × 2 BCR BETA-CAROTENE × 24 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 CL CHLORIDE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；298 K；4% PEG 2000, 5 mM CaCl2, 100 mM PIPES, pH 7.0, batch, temperature 298K
Resolution 6.56 Å R-free 0.385
4IXQ RT fs X-ray diffraction of Photosystem II, dark state Deposited 2013-01-27 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain T 1–32（32 aa）
Chain t 1–32（32 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 LMT DODECYL-BETA-D-MALTOSIDE × 14 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.70 Å R-free 0.314
4IXR RT fs X-ray diffraction of Photosystem II, first illuminated state Deposited 2013-01-27 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain T 1–32（32 aa）
Chain t 1–32（32 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 LMT DODECYL-BETA-D-MALTOSIDE × 14 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.90 Å R-free 0.313
4PBU Serial Time-resolved crystallography of Photosystem II using a femtosecond X-ray laser The S1 state Deposited 2014-04-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain T 1–30（30 aa）
Chain t 1–30（30 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 CA CALCIUM ION × 7 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 5.00 Å R-free 0.262
4PJ0 Structure of T.elongatus Photosystem II, rows of dimers crystal packing Deposited 2014-05-10 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain T 1–32（32 aa）
Chain t 1–32（32 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE FE (III) ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SO4 SULFATE ION × 10 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 29 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；pH 7.5；293 K；Batch crystallisation: 2 mM Chl a equivalent of PSIIcc mixed 1:1 with: 0.1 M TRIS (pH 7.5), 0.1 M (NH4)2SO4, 15 - 18 % PEG 5000 MME, post-crystallisation dehydration
Resolution 2.44 Å R-free 0.256
4RVY Serial Time resolved crystallography of Photosystem II using a femtosecond X-ray laser. The S state after two flashes (S3) Deposited 2014-11-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain T 1–30（30 aa）
Chain t 1–30（30 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 CA CALCIUM ION × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions Free interface diffusion；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000 , Free interface diffusion, temperature 283K, temperature 283.0K
Resolution 5.50 Å R-free 0.291
4TNH RT XFEL structure of Photosystem II in the dark state at 4.9 A resolution Deposited 2014-06-04 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain T 1–32（32 aa）
Chain t 1–32（32 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.90 Å R-free 0.292
4TNI RT XFEL structure of Photosystem II 500 ms after the third illumination at 4.6 A resolution Deposited 2014-06-04 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain T 1–32（32 aa）
Chain t 1–32（32 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 2 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.60 Å R-free 0.284
4TNJ RT XFEL structure of Photosystem II 500 ms after the 2nd illumination (2F) at 4.5 A resolution Deposited 2014-06-04 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain T 1–32（32 aa）
Chain t 1–32（32 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.50 Å R-free 0.284
4TNK RT XFEL structure of Photosystem II 250 microsec after the third illumination at 5.2 A resolution Deposited 2014-06-04 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain T 1–32（32 aa）
Chain t 1–32（32 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.20 Å R-free 0.289
4V62 Crystal Structure of cyanobacterial Photosystem II Deposited 2008-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain AT 1–32（32 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 3 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 13 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 7 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 CL CHLORIDE ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 7 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG2000, PIPES, magnesium chloride, pH7.0, batch, temperature 298K
Resolution 2.90 Å R-free 0.292
4V62 Crystal Structure of cyanobacterial Photosystem II Deposited 2008-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain BT 1–32（32 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 3 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 11 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 7 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 CL CHLORIDE ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 7 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG2000, PIPES, magnesium chloride, pH7.0, batch, temperature 298K
Resolution 2.90 Å R-free 0.292
4V82 Crystal structure of cyanobacterial Photosystem II in complex with terbutryn Deposited 2010-11-30 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain AT 1–32（32 aa）
Chain BT 1–32（32 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 70 MST 2-T-BUTYLAMINO-4-ETHYLAMINO-6-METHYLTHIO-S-TRIAZINE × 2 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 LMT DODECYL-BETA-D-MALTOSIDE × 16 DMS DIMETHYL SULFOXIDE × 8 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；Pipes, CaCl2, DMSO, PEG 2000, pH 7.0, batch, temperature 298K
Resolution 3.20 Å R-free 0.299
5E79 Macromolecular diffractive imaging using imperfect crystals Deposited 2015-10-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain T 1–30（30 aa）
Chain t 1–30（30 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 CA CALCIUM ION × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；283.18 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 3.50 Å R-free 0.331
5E7C Macromolecular diffractive imaging using imperfect crystals - Bragg data Deposited 2015-10-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain T 1–30（30 aa）
Chain t 1–30（30 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 FE2 FE (II) ION × 2 CA CALCIUM ION × 6 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 4.50 Å R-free 0.275
5H2F Crystal structure of the PsbM-deletion mutant of photosystem II Deposited 2016-10-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain T 1–30（30 aa） Fragment：UNP RESIDUES 1-30
Non-standard monomer：Yes (specific site not provided by mmCIF) OEX CA-MN4-O5 CLUSTER × 1 FE2 FE (II) ION × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 3 LMT DODECYL-BETA-D-MALTOSIDE × 6 UNL UNKNOWN LIGAND × 13 DMS DIMETHYL SULFOXIDE × 67 BCT BICARBONATE ION × 1 CA CALCIUM ION × 2 HTG heptyl 1-thio-beta-D-glucopyranoside × 9 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6.1；285 K；PEG1450, sodium chloride, calcium chloride, magnesium sulfate
Resolution 2.20 Å R-free 0.226
5H2F Crystal structure of the PsbM-deletion mutant of photosystem II Deposited 2016-10-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain t 1–30（30 aa） Fragment：UNP RESIDUES 1-30
Non-standard monomer：Yes (specific site not provided by mmCIF) OEX CA-MN4-O5 CLUSTER × 1 FE2 FE (II) ION × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 5 LMT DODECYL-BETA-D-MALTOSIDE × 5 UNL UNKNOWN LIGAND × 14 DMS DIMETHYL SULFOXIDE × 66 BCT BICARBONATE ION × 1 CA CALCIUM ION × 3 HTG heptyl 1-thio-beta-D-glucopyranoside × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6.1；285 K；PEG1450, sodium chloride, calcium chloride, magnesium sulfate
Resolution 2.20 Å R-free 0.226
5KAI NH3-bound RT XFEL structure of Photosystem II 500 ms after the 2nd illumination (2F) at 2.8 A resolution Deposited 2016-06-01 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain T 2–32（31 aa）
Chain t 2–32（31 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 UNL UNKNOWN LIGAND × 23 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol
Resolution 2.80 Å R-free 0.300
5MX2 Photosystem II depleted of the Mn4CaO5 cluster at 2.55 A resolution Deposited 2017-01-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain T 1–32（32 aa）
Not recorded CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 11 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LFA EICOSANE × 19 FE FE (III) ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 4 BCT BICARBONATE ION × 1 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 7.5；291 K；equal volumes of buffer: 0.1 M Tris-HCl pH 7.5, 0.1 M (NH4)2SO4, 14-18 % PEG 5000 MME and protein: 2 mM chlorophyll equivalent of PSII in C12E8 detergent, post crystallisation treatment
Resolution 2.20 Å R-free 0.260
5MX2 Photosystem II depleted of the Mn4CaO5 cluster at 2.55 A resolution Deposited 2017-01-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain t 1–32（32 aa）
Not recorded CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 11 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LFA EICOSANE × 20 FE FE (III) ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 4 BCT BICARBONATE ION × 1 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 7.5；291 K；equal volumes of buffer: 0.1 M Tris-HCl pH 7.5, 0.1 M (NH4)2SO4, 14-18 % PEG 5000 MME and protein: 2 mM chlorophyll equivalent of PSII in C12E8 detergent, post crystallisation treatment
Resolution 2.20 Å R-free 0.260
5TIS Room temperature XFEL structure of the native, doubly-illuminated photosystem II complex Deposited 2016-10-03 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain T 1–32（32 aa）
Chain t 1–32（32 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 UNL UNKNOWN LIGAND × 33 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol
Resolution 2.25 Å R-free 0.231
5ZZN Crystal structure of photosystem II from an SQDG-deficient mutant of Thermosynechococcus elongatus Deposited 2018-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain T 1–30（30 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) OEX CA-MN4-O5 CLUSTER × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 5 UNL UNKNOWN LIGAND × 27 LMT DODECYL-BETA-D-MALTOSIDE × 9 DMS DIMETHYL SULFOXIDE × 48 CA CALCIUM ION × 3 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 HTG heptyl 1-thio-beta-D-glucopyranoside × 12 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 GOL GLYCEROL × 2 FE2 FE (II) ION × 1 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20MM NACL, 10MM CACL2, 40MM MGSO4, 20MM MES BUFFER, PH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPERATURE 285K
Resolution 2.10 Å R-free 0.197
5ZZN Crystal structure of photosystem II from an SQDG-deficient mutant of Thermosynechococcus elongatus Deposited 2018-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain t 1–30（30 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) OEX CA-MN4-O5 CLUSTER × 1 CL CHLORIDE ION × 3 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 5 UNL UNKNOWN LIGAND × 25 LMT DODECYL-BETA-D-MALTOSIDE × 10 DMS DIMETHYL SULFOXIDE × 47 CA CALCIUM ION × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 HTG heptyl 1-thio-beta-D-glucopyranoside × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 GOL GLYCEROL × 3 FE2 FE (II) ION × 1 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20MM NACL, 10MM CACL2, 40MM MGSO4, 20MM MES BUFFER, PH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPERATURE 285K
Resolution 2.10 Å R-free 0.197
6DHE RT XFEL structure of the dark-stable state of Photosystem II (0F, S1-rich) at 2.05 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain T 1–30（30 aa）
Chain t 1–30（30 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 8CT (6'R,11cis,11'cis,13cis,15cis)-4',5'-didehydro-5',6'-dihydro-beta,beta-carotene × 12 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 BCR BETA-CAROTENE × 10 UNL UNKNOWN LIGAND × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.05 Å R-free 0.241
6DHF RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.08 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain T 1–30（30 aa）
Chain t 1–30（30 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 UNL UNKNOWN LIGAND × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.247
6DHG RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.5 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain T 1–30（30 aa）
Chain t 1–30（30 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.50 Å R-free 0.246
6DHH RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.2 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain T 1–30（30 aa）
Chain t 1–30（30 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.20 Å R-free 0.264
6DHO RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.07 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain T 1–30（30 aa）
Chain t 1–30（30 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.07 Å R-free 0.247
6DHP RT XFEL structure of the three-flash state of Photosystem II (3F, S0-rich) at 2.04 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain T 1–30（30 aa）
Chain t 1–30（30 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 UNL UNKNOWN LIGAND × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.04 Å R-free 0.248
6W1O RT XFEL structure of the dark-stable state of Photosystem II (0F, S1-rich) at 2.08 Angstrom resolution Deposited 2020-03-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain T 2–30（29 aa）
Chain t 2–30（29 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.240
6W1P RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.26 Angstrom resolution Deposited 2020-03-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain T 2–30（29 aa）
Chain t 2–30（29 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.26 Å R-free 0.241
6W1Q RT XFEL structure of Photosystem II 50 microseconds after the second illumination at 2.27 Angstrom resolution Deposited 2020-03-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain T 2–30（29 aa）
Chain t 2–30（29 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 BCR BETA-CAROTENE × 22 STE STEARIC ACID × 35 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 HEC HEME C × 4 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.27 Å R-free 0.249
6W1R RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.23 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain T 2–32（31 aa）
Chain t 2–32（31 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.23 Å R-free 0.235
6W1T RT XFEL structure of Photosystem II 250 microseconds after the second illumination at 2.01 Angstrom resolution Deposited 2020-03-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain T 2–30（29 aa）
Chain t 2–30（29 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.01 Å R-free 0.228
6W1U RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.09 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain T 2–32（31 aa）
Chain t 2–32（31 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
6W1V RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain T 2–32（31 aa）
Chain t 2–32（31 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 STE STEARIC ACID × 38 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 PHO PHEOPHYTIN A × 4 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.241
7NHO Structure of PSII-M Deposited 2021-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain T 1–32（32 aa）
Not recorded FE FE (III) ION × 1 MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 BCT BICARBONATE ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.66 Å
7NHP Structure of PSII-I (PSII with Psb27, Psb28, and Psb34) Deposited 2021-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain T 1–32（32 aa）
Not recorded MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 FE FE (III) ION × 1 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.72 Å
7NHQ Structure of PSII-I prime (PSII with Psb28, and Psb34) Deposited 2021-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 17 PDB declaration: heptadecameric
Chain T 1–32（32 aa）
Not recorded FE FE (III) ION × 1 MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.68 Å
7RF1 RT XFEL structure of Photosystem II averaged across all S-states at 1.89 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain T 2–32（31 aa）
Chain t 2–32（31 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 STE STEARIC ACID × 55 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 1.89 Å R-free 0.214
7RF2 RT XFEL structure of dark-stable state of Photosystem II (0F, S1 rich) at 2.08 Angstrom Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain T 2–32（31 aa）
Chain t 2–32（31 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 33 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.238
7RF3 RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.26 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain T 2–32（31 aa）
Chain t 2–32（31 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.26 Å R-free 0.238
7RF4 RT XFEL structure of Photosystem II 50 microseconds after the second illumination at 2.27 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain T 2–32（31 aa）
Chain t 2–32（31 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 BCR BETA-CAROTENE × 22 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.27 Å R-free 0.244
7RF5 RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.23 Angstrom resolution Deposited 2021-07-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain T 1–32（32 aa）
Chain t 1–32（32 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.23 Å R-free 0.233
7RF6 RT XFEL structure of Photosystem II 250 microseconds after the second illumination at 2.01 Angstrom resolution Deposited 2021-07-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain T 1–32（32 aa）
Chain t 1–32（32 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.01 Å R-free 0.227
7RF7 RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.09 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain T 2–32（31 aa）
Chain t 2–32（31 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
7RF8 RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain T 2–32（31 aa）
Chain t 2–32（31 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
7YQ2 Crystal structure of photosystem II expressing psbA2 gene only Deposited 2022-08-05 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 39 PDB declaration: 39-meric
Chain T 1–32（32 aa）
Chain t 1–32（32 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 UNL UNKNOWN LIGAND × 77 LMT DODECYL-BETA-D-MALTOSIDE × 19 GOL GLYCEROL × 10 BCT BICARBONATE ION × 3 DMS DIMETHYL SULFOXIDE × 116 CA CALCIUM ION × 7 HTG heptyl 1-thio-beta-D-glucopyranoside × 31 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 9 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 MG MAGNESIUM ION × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20mM NaCl, 10mM CaCl2, 40mM MgSO4, 20mM Mes BUFFER, pH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPARATURE 285K
Resolution 1.90 Å R-free 0.184
7YQ7 Crystal structure of photosystem II expressing psbA3 gene only Deposited 2022-08-05 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 39 PDB declaration: 39-meric
Chain T 1–32（32 aa）
Chain t 1–32（32 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 UNL UNKNOWN LIGAND × 82 GOL GLYCEROL × 11 LMT DODECYL-BETA-D-MALTOSIDE × 18 BCT BICARBONATE ION × 4 DMS DIMETHYL SULFOXIDE × 71 CA CALCIUM ION × 5 HTG heptyl 1-thio-beta-D-glucopyranoside × 18 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 MG MAGNESIUM ION × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20mM NaCl, 10mM CaCl2, 40mM MgSO4, 20mM MES BUFFER pH 6.0, MICRO-BATCH METHOD UNDER OIL, TMPERATURE 285K
Resolution 1.90 Å R-free 0.177
8EZ5 RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2022-10-31 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain T 1–32（32 aa）
Chain t 1–32（32 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.234
8F4C RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain T 2–32（31 aa）
Chain t 2–32（31 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.224
8F4D RT XFEL structure of Photosystem II 50 microseconds after the third illumination at 2.15 Angstrom resolution Deposited 2022-11-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain T 1–32（32 aa）
Chain t 1–32（32 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.15 Å R-free 0.243
8F4E RT XFEL structure of Photosystem II 250 microseconds after the third illumination at 2.09 Angstrom resolution Deposited 2022-11-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain T 1–32（32 aa）
Chain t 1–32（32 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.233
8F4F RT XFEL structure of Photosystem II 500 microseconds after the third illumination at 2.03 Angstrom resolution Deposited 2022-11-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain T 1–32（32 aa）
Chain t 1–32（32 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.03 Å R-free 0.248
8F4G RT XFEL structure of Photosystem II 730 microseconds after the third illumination at 2.03 Angstrom resolution Deposited 2022-11-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain T 1–32（32 aa）
Chain t 1–32（32 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.03 Å R-free 0.223
8F4H RT XFEL structure of Photosystem II 1200 microseconds after the third illumination at 2.10 Angstrom resolution Deposited 2022-11-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain T 1–32（32 aa）
Chain t 1–32（32 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.10 Å R-free 0.232
8F4I RT XFEL structure of Photosystem II 2000 microseconds after the third illumination at 2.00 Angstrom resolution Deposited 2022-11-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain T 1–32（32 aa）
Chain t 1–32（32 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.232
8F4J RT XFEL structure of Photosystem II 4000 microseconds after the third illumination at 2.00 Angstrom resolution Deposited 2022-11-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain T 1–32（32 aa）
Chain t 1–32（32 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.226
8F4K RT XFEL structure of the three-flash state of Photosystem II (3F, S0-rich) at 2.16 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain T 2–32（31 aa）
Chain t 2–32（31 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.16 Å R-free 0.241
9EVX cryoEM structure of Photosystem II averaged across S2-S3 states at 1.71 Angstrom resolution Deposited 2024-04-02 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain T 1–32（32 aa）
Chain t 1–32（32 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 STE STEARIC ACID × 42 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 PHO PHEOPHYTIN A × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；100mM MES pH6.5, 5mM CaCl2, 5% glycerol, 0.03% DDM
cryo-EM vitrification conditions Cryogen ETHANE；The sample-on-grid was illuminated with one and two flashes of light
Resolution 1.71 Å
9G6F Inactive PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain T 1–32（32 aa）
Chain t 1–32（32 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LFA EICOSANE × 33 PLM PALMITIC ACID × 24 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 8 LMT DODECYL-BETA-D-MALTOSIDE × 13 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å
9G6G Semi-active PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric
Chain T 1–32（32 aa）
Chain t 1–32（32 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CL CHLORIDE ION × 3 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 LFA EICOSANE × 30 OEX CA-MN4-O5 CLUSTER × 1 LMT DODECYL-BETA-D-MALTOSIDE × 17 BCT BICARBONATE ION × 2 PLM PALMITIC ACID × 29 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 GOL GLYCEROL × 1 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 3 RRX (3R)-beta,beta-caroten-3-ol × 2 CA CALCIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å
9G6H active PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain T 1–32（32 aa）
Chain t 1–32（32 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 PLM PALMITIC ACID × 35 LFA EICOSANE × 24 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 GOL GLYCEROL × 2 LMT DODECYL-BETA-D-MALTOSIDE × 17 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 RRX (3R)-beta,beta-caroten-3-ol × 2 CA CALCIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å

63 other PDB entries and 68 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PSBT_THEEB
Isoform —
PDB entities 14
Chains and sequence ranges Author chain T； PDBConstruct 2–32； UniProt 2–32 Author chain t； PDBConstruct 2–32； UniProt 2–32

Photosystem II 12 kDa extrinsic protein

OrganismNot specified

UniProt Q9F1L5

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 40 PDB declaration: 40-meric（40） Consistent with protein copy count Chain U； UniProt 1–134 Chain u； UniProt 1–134 Not recorded Photosystem II protein D1 1 × 2 （P0A444） Photosystem II CP47 reaction center protein × 2 （Q8DIQ1） Photosystem II CP43 reaction center protein × 2 （Q8DIF8） Photosystem II D2 protein × 2 （Q8CM25） Cytochrome b559 subunit alpha × 2 （Q8DIP0） Cytochrome b559 subunit beta × 2 （Q8DIN9） Photosystem II reaction center protein H × 2 （Q8DJ43） Photosystem II reaction center protein I × 2 （Q8DJZ6） Photosystem II reaction center protein J × 2 （P59087） Photosystem II reaction center protein K × 2 （Q9F1K9） Photosystem II reaction center protein L × 2 （Q8DIN8） Photosystem II reaction center protein M × 2 （Q8DHA7） Photosystem II manganese-stabilizing polypeptide × 2 （P0A431） Photosystem II reaction center protein T × 2 （Q8DIQ0） Cytochrome c-550 × 2 （P0A386） Photosystem II reaction center protein Ycf12 × 2 （Q8DJI1） Photosystem II reaction center X protein × 2 （Q9F1R6） Photosystem II reaction center protein Z × 2 （Q8DHJ2） Photosystem II protein Y × 2 （Q8DKM3） OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 UNL UNKNOWN LIGAND × 17 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION X-ray crystallization conditions：BATCH MODE；pH 7.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol. Resolution 3.00 Å R-free 0.303

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5KAF Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
11YO Single-conformation model re-refinement of 2F/S3-rich PSII intermediate structure at 2.09 Angstrom resolution Deposited 2026-03-18 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain U 1–134（134 aa）
Chain u 1–134（134 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 38 OEX CA-MN4-O5 CLUSTER × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 PHO PHEOPHYTIN A × 4 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.243
1S5L Architecture of the photosynthetic oxygen evolving center Deposited 2004-01-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain U 1–134（134 aa）
Not recorded FE FE (III) ION × 1 BCT BICARBONATE ION × 2 OEC OXYGEN EVOLVING SYSTEM × 1 CLA CHLOROPHYLL A × 36 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMT DODECYL-BETA-D-MALTOSIDE × 1 BCR BETA-CAROTENE × 7 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pseudo-batch hanging drop；pH 7.5；291 K；100mM HEPES, 100mM (NH4)2SO4, PEG 4000, C12E8, trimethyl lead acetate, pH 7.5, pseudo-batch hanging drop, temperature 291K
Resolution 3.50 Å R-free 0.342
1S5L Architecture of the photosynthetic oxygen evolving center Deposited 2004-01-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain u 1–134（134 aa）
Not recorded FE FE (III) ION × 1 BCT BICARBONATE ION × 2 OEC OXYGEN EVOLVING SYSTEM × 1 CLA CHLOROPHYLL A × 36 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMT DODECYL-BETA-D-MALTOSIDE × 1 BCR BETA-CAROTENE × 7 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pseudo-batch hanging drop；pH 7.5；291 K；100mM HEPES, 100mM (NH4)2SO4, PEG 4000, C12E8, trimethyl lead acetate, pH 7.5, pseudo-batch hanging drop, temperature 291K
Resolution 3.50 Å R-free 0.342
2AXT Crystal Structure of Photosystem II from Thermosynechococcus elongatus Deposited 2005-09-06 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 36 PDB declaration: 36-meric
Chain U 31–134（104 aa）
Chain u 31–134（104 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PQ9 5-[(2E,6E,10E,14E,18E,22E)-3,7,11,15,19,23,27-HEPTAMETHYLOCTACOSA-2,6,10,14,18,22,26-HEPTAENYL]-2,3-DIMETHYLBENZO-1,4-QUINONE × 4 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 22 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LMT DODECYL-BETA-D-MALTOSIDE × 6 MGE (1S)-2-(ALPHA-L-ALLOPYRANOSYLOXY)-1-[(TRIDECANOYLOXY)METHYL]ETHYL PALMITATE × 12 UNL UNKNOWN LIGAND × 34 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG 2000, PIPES, magnesium chloride, pH 7.0, batch, temperature 298K
Resolution 3.00 Å R-free 0.286
3KZI Crystal Structure of Monomeric Form of Cyanobacterial Photosystem II Deposited 2009-12-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain U 31–134（104 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 11 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 LMT DODECYL-BETA-D-MALTOSIDE × 7 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 BCT BICARBONATE ION × 1 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；291 K；PEG 400, PIPES, MES, CaCl2, pH 7, batch, temperature 291.0K
Resolution 3.60 Å R-free 0.308
4FBY fs X-ray diffraction of Photosystem II Deposited 2012-05-23 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain U 31–134（104 aa）
Chain h 31–134（104 aa）
Not recorded CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 OEC OXYGEN EVOLVING SYSTEM × 2 FE2 FE (II) ION × 2 BCR BETA-CAROTENE × 24 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 CL CHLORIDE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；298 K；4% PEG 2000, 5 mM CaCl2, 100 mM PIPES, pH 7.0, batch, temperature 298K
Resolution 6.56 Å R-free 0.385
4IXQ RT fs X-ray diffraction of Photosystem II, dark state Deposited 2013-01-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain U 1–134（134 aa）
Chain u 1–134（134 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 LMT DODECYL-BETA-D-MALTOSIDE × 14 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.70 Å R-free 0.314
4IXR RT fs X-ray diffraction of Photosystem II, first illuminated state Deposited 2013-01-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain U 1–134（134 aa）
Chain u 1–134（134 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 LMT DODECYL-BETA-D-MALTOSIDE × 14 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.90 Å R-free 0.313
4PBU Serial Time-resolved crystallography of Photosystem II using a femtosecond X-ray laser The S1 state Deposited 2014-04-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain U 38–134（97 aa）
Chain u 38–134（97 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 CA CALCIUM ION × 7 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 5.00 Å R-free 0.262
4PJ0 Structure of T.elongatus Photosystem II, rows of dimers crystal packing Deposited 2014-05-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain U 1–134（134 aa）
Chain u 1–134（134 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE FE (III) ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SO4 SULFATE ION × 10 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 29 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；pH 7.5；293 K；Batch crystallisation: 2 mM Chl a equivalent of PSIIcc mixed 1:1 with: 0.1 M TRIS (pH 7.5), 0.1 M (NH4)2SO4, 15 - 18 % PEG 5000 MME, post-crystallisation dehydration
Resolution 2.44 Å R-free 0.256
4RVY Serial Time resolved crystallography of Photosystem II using a femtosecond X-ray laser. The S state after two flashes (S3) Deposited 2014-11-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain U 38–134（97 aa）
Chain u 38–134（97 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 CA CALCIUM ION × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions Free interface diffusion；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000 , Free interface diffusion, temperature 283K, temperature 283.0K
Resolution 5.50 Å R-free 0.291
4TNH RT XFEL structure of Photosystem II in the dark state at 4.9 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain U 1–134（134 aa）
Chain u 1–134（134 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.90 Å R-free 0.292
4TNI RT XFEL structure of Photosystem II 500 ms after the third illumination at 4.6 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain U 1–134（134 aa）
Chain u 1–134（134 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 2 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.60 Å R-free 0.284
4TNJ RT XFEL structure of Photosystem II 500 ms after the 2nd illumination (2F) at 4.5 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain U 1–134（134 aa）
Chain u 1–134（134 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.50 Å R-free 0.284
4TNK RT XFEL structure of Photosystem II 250 microsec after the third illumination at 5.2 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain U 1–134（134 aa）
Chain u 1–134（134 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.20 Å R-free 0.289
4V62 Crystal Structure of cyanobacterial Photosystem II Deposited 2008-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain AU 31–134（104 aa） Fragment：UNP residues 31-134
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 3 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 13 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 7 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 CL CHLORIDE ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 7 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG2000, PIPES, magnesium chloride, pH7.0, batch, temperature 298K
Resolution 2.90 Å R-free 0.292
4V62 Crystal Structure of cyanobacterial Photosystem II Deposited 2008-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain BU 31–134（104 aa） Fragment：UNP residues 31-134
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 3 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 11 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 7 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 CL CHLORIDE ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 7 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG2000, PIPES, magnesium chloride, pH7.0, batch, temperature 298K
Resolution 2.90 Å R-free 0.292
4V82 Crystal structure of cyanobacterial Photosystem II in complex with terbutryn Deposited 2010-11-30 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain AU 31–134（104 aa）
Chain BU 31–134（104 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 70 MST 2-T-BUTYLAMINO-4-ETHYLAMINO-6-METHYLTHIO-S-TRIAZINE × 2 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 LMT DODECYL-BETA-D-MALTOSIDE × 16 DMS DIMETHYL SULFOXIDE × 8 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；Pipes, CaCl2, DMSO, PEG 2000, pH 7.0, batch, temperature 298K
Resolution 3.20 Å R-free 0.299
5E79 Macromolecular diffractive imaging using imperfect crystals Deposited 2015-10-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain U 38–134（97 aa）
Chain u 38–134（97 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 CA CALCIUM ION × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；283.18 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 3.50 Å R-free 0.331
5E7C Macromolecular diffractive imaging using imperfect crystals - Bragg data Deposited 2015-10-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain U 38–134（97 aa）
Chain u 38–134（97 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 FE2 FE (II) ION × 2 CA CALCIUM ION × 6 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 4.50 Å R-free 0.275
5H2F Crystal structure of the PsbM-deletion mutant of photosystem II Deposited 2016-10-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain U 38–134（97 aa） Fragment：UNP RESIDUES 38-134
Not recorded OEX CA-MN4-O5 CLUSTER × 1 FE2 FE (II) ION × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 3 LMT DODECYL-BETA-D-MALTOSIDE × 6 UNL UNKNOWN LIGAND × 13 DMS DIMETHYL SULFOXIDE × 67 BCT BICARBONATE ION × 1 CA CALCIUM ION × 2 HTG heptyl 1-thio-beta-D-glucopyranoside × 9 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6.1；285 K；PEG1450, sodium chloride, calcium chloride, magnesium sulfate
Resolution 2.20 Å R-free 0.226
5H2F Crystal structure of the PsbM-deletion mutant of photosystem II Deposited 2016-10-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain u 38–134（97 aa） Fragment：UNP RESIDUES 38-134
Not recorded OEX CA-MN4-O5 CLUSTER × 1 FE2 FE (II) ION × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 5 LMT DODECYL-BETA-D-MALTOSIDE × 5 UNL UNKNOWN LIGAND × 14 DMS DIMETHYL SULFOXIDE × 66 BCT BICARBONATE ION × 1 CA CALCIUM ION × 3 HTG heptyl 1-thio-beta-D-glucopyranoside × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6.1；285 K；PEG1450, sodium chloride, calcium chloride, magnesium sulfate
Resolution 2.20 Å R-free 0.226
5KAI NH3-bound RT XFEL structure of Photosystem II 500 ms after the 2nd illumination (2F) at 2.8 A resolution Deposited 2016-06-01 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain U 1–134（134 aa）
Chain u 1–134（134 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 UNL UNKNOWN LIGAND × 23 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol
Resolution 2.80 Å R-free 0.300
5MX2 Photosystem II depleted of the Mn4CaO5 cluster at 2.55 A resolution Deposited 2017-01-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain U 1–134（134 aa）
Not recorded CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 11 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LFA EICOSANE × 19 FE FE (III) ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 4 BCT BICARBONATE ION × 1 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 7.5；291 K；equal volumes of buffer: 0.1 M Tris-HCl pH 7.5, 0.1 M (NH4)2SO4, 14-18 % PEG 5000 MME and protein: 2 mM chlorophyll equivalent of PSII in C12E8 detergent, post crystallisation treatment
Resolution 2.20 Å R-free 0.260
5MX2 Photosystem II depleted of the Mn4CaO5 cluster at 2.55 A resolution Deposited 2017-01-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain u 1–134（134 aa）
Not recorded CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 11 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LFA EICOSANE × 20 FE FE (III) ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 4 BCT BICARBONATE ION × 1 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 7.5；291 K；equal volumes of buffer: 0.1 M Tris-HCl pH 7.5, 0.1 M (NH4)2SO4, 14-18 % PEG 5000 MME and protein: 2 mM chlorophyll equivalent of PSII in C12E8 detergent, post crystallisation treatment
Resolution 2.20 Å R-free 0.260
5TIS Room temperature XFEL structure of the native, doubly-illuminated photosystem II complex Deposited 2016-10-03 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain U 1–134（134 aa）
Chain u 1–134（134 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 UNL UNKNOWN LIGAND × 33 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol
Resolution 2.25 Å R-free 0.231
5ZZN Crystal structure of photosystem II from an SQDG-deficient mutant of Thermosynechococcus elongatus Deposited 2018-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain U 38–134（97 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 5 UNL UNKNOWN LIGAND × 27 LMT DODECYL-BETA-D-MALTOSIDE × 9 DMS DIMETHYL SULFOXIDE × 48 CA CALCIUM ION × 3 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 HTG heptyl 1-thio-beta-D-glucopyranoside × 12 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 GOL GLYCEROL × 2 FE2 FE (II) ION × 1 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20MM NACL, 10MM CACL2, 40MM MGSO4, 20MM MES BUFFER, PH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPERATURE 285K
Resolution 2.10 Å R-free 0.197
5ZZN Crystal structure of photosystem II from an SQDG-deficient mutant of Thermosynechococcus elongatus Deposited 2018-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain u 38–134（97 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 1 CL CHLORIDE ION × 3 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 5 UNL UNKNOWN LIGAND × 25 LMT DODECYL-BETA-D-MALTOSIDE × 10 DMS DIMETHYL SULFOXIDE × 47 CA CALCIUM ION × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 HTG heptyl 1-thio-beta-D-glucopyranoside × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 GOL GLYCEROL × 3 FE2 FE (II) ION × 1 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20MM NACL, 10MM CACL2, 40MM MGSO4, 20MM MES BUFFER, PH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPERATURE 285K
Resolution 2.10 Å R-free 0.197
6DHE RT XFEL structure of the dark-stable state of Photosystem II (0F, S1-rich) at 2.05 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain U 38–134（97 aa）
Chain u 38–134（97 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 8CT (6'R,11cis,11'cis,13cis,15cis)-4',5'-didehydro-5',6'-dihydro-beta,beta-carotene × 12 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 BCR BETA-CAROTENE × 10 UNL UNKNOWN LIGAND × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.05 Å R-free 0.241
6DHF RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.08 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain U 38–134（97 aa）
Chain u 38–134（97 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 UNL UNKNOWN LIGAND × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.247
6DHG RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.5 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain U 38–134（97 aa）
Chain u 38–134（97 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.50 Å R-free 0.246
6DHH RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.2 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain U 38–134（97 aa）
Chain u 38–134（97 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.20 Å R-free 0.264
6DHO RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.07 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain U 38–134（97 aa）
Chain u 38–134（97 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.07 Å R-free 0.247
6DHP RT XFEL structure of the three-flash state of Photosystem II (3F, S0-rich) at 2.04 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain U 38–134（97 aa）
Chain u 38–134（97 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 UNL UNKNOWN LIGAND × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.04 Å R-free 0.248
6W1O RT XFEL structure of the dark-stable state of Photosystem II (0F, S1-rich) at 2.08 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain U 1–134（134 aa）
Chain u 1–134（134 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.240
6W1P RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.26 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain U 1–134（134 aa）
Chain u 1–134（134 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.26 Å R-free 0.241
6W1Q RT XFEL structure of Photosystem II 50 microseconds after the second illumination at 2.27 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain U 1–134（134 aa）
Chain u 1–134（134 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 BCR BETA-CAROTENE × 22 STE STEARIC ACID × 35 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 HEC HEME C × 4 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.27 Å R-free 0.249
6W1R RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.23 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain U 1–134（134 aa）
Chain u 1–134（134 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.23 Å R-free 0.235
6W1T RT XFEL structure of Photosystem II 250 microseconds after the second illumination at 2.01 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain U 1–134（134 aa）
Chain u 1–134（134 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.01 Å R-free 0.228
6W1U RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.09 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain U 1–134（134 aa）
Chain u 1–134（134 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
6W1V RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain U 1–134（134 aa）
Chain u 1–134（134 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 STE STEARIC ACID × 38 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 PHO PHEOPHYTIN A × 4 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.241
7RF1 RT XFEL structure of Photosystem II averaged across all S-states at 1.89 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain U 1–134（134 aa）
Chain u 1–134（134 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 STE STEARIC ACID × 55 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 1.89 Å R-free 0.214
7RF2 RT XFEL structure of dark-stable state of Photosystem II (0F, S1 rich) at 2.08 Angstrom Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain U 1–134（134 aa）
Chain u 1–134（134 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 33 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.238
7RF3 RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.26 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain U 1–134（134 aa）
Chain u 1–134（134 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.26 Å R-free 0.238
7RF4 RT XFEL structure of Photosystem II 50 microseconds after the second illumination at 2.27 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain U 1–134（134 aa）
Chain u 1–134（134 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 BCR BETA-CAROTENE × 22 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.27 Å R-free 0.244
7RF5 RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.23 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain U 1–134（134 aa）
Chain u 1–134（134 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.23 Å R-free 0.233
7RF6 RT XFEL structure of Photosystem II 250 microseconds after the second illumination at 2.01 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain U 1–134（134 aa）
Chain u 1–134（134 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.01 Å R-free 0.227
7RF7 RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.09 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain U 1–134（134 aa）
Chain u 1–134（134 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
7RF8 RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain U 1–134（134 aa）
Chain u 1–134（134 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
7YQ2 Crystal structure of photosystem II expressing psbA2 gene only Deposited 2022-08-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 39 PDB declaration: 39-meric
Chain U 1–134（134 aa）
Chain u 1–134（134 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 UNL UNKNOWN LIGAND × 77 LMT DODECYL-BETA-D-MALTOSIDE × 19 GOL GLYCEROL × 10 BCT BICARBONATE ION × 3 DMS DIMETHYL SULFOXIDE × 116 CA CALCIUM ION × 7 HTG heptyl 1-thio-beta-D-glucopyranoside × 31 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 9 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 MG MAGNESIUM ION × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20mM NaCl, 10mM CaCl2, 40mM MgSO4, 20mM Mes BUFFER, pH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPARATURE 285K
Resolution 1.90 Å R-free 0.184
7YQ7 Crystal structure of photosystem II expressing psbA3 gene only Deposited 2022-08-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 39 PDB declaration: 39-meric
Chain U 1–134（134 aa）
Chain u 1–134（134 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 UNL UNKNOWN LIGAND × 82 GOL GLYCEROL × 11 LMT DODECYL-BETA-D-MALTOSIDE × 18 BCT BICARBONATE ION × 4 DMS DIMETHYL SULFOXIDE × 71 CA CALCIUM ION × 5 HTG heptyl 1-thio-beta-D-glucopyranoside × 18 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 MG MAGNESIUM ION × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20mM NaCl, 10mM CaCl2, 40mM MgSO4, 20mM MES BUFFER pH 6.0, MICRO-BATCH METHOD UNDER OIL, TMPERATURE 285K
Resolution 1.90 Å R-free 0.177
8EZ5 RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2022-10-31 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain U 1–134（134 aa）
Chain u 1–134（134 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.234
8F4C RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain U 1–134（134 aa）
Chain u 1–134（134 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.224
8F4D RT XFEL structure of Photosystem II 50 microseconds after the third illumination at 2.15 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain U 1–134（134 aa）
Chain u 1–134（134 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.15 Å R-free 0.243
8F4E RT XFEL structure of Photosystem II 250 microseconds after the third illumination at 2.09 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain U 1–134（134 aa）
Chain u 1–134（134 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.233
8F4F RT XFEL structure of Photosystem II 500 microseconds after the third illumination at 2.03 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain U 1–134（134 aa）
Chain u 1–134（134 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.03 Å R-free 0.248
8F4G RT XFEL structure of Photosystem II 730 microseconds after the third illumination at 2.03 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain U 1–134（134 aa）
Chain u 1–134（134 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.03 Å R-free 0.223
8F4H RT XFEL structure of Photosystem II 1200 microseconds after the third illumination at 2.10 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain U 1–134（134 aa）
Chain u 1–134（134 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.10 Å R-free 0.232
8F4I RT XFEL structure of Photosystem II 2000 microseconds after the third illumination at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain U 1–134（134 aa）
Chain u 1–134（134 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.232
8F4J RT XFEL structure of Photosystem II 4000 microseconds after the third illumination at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain U 1–134（134 aa）
Chain u 1–134（134 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.226
8F4K RT XFEL structure of the three-flash state of Photosystem II (3F, S0-rich) at 2.16 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain U 1–134（134 aa）
Chain u 1–134（134 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.16 Å R-free 0.241
9EVX cryoEM structure of Photosystem II averaged across S2-S3 states at 1.71 Angstrom resolution Deposited 2024-04-02 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain U 1–134（134 aa）
Chain u 1–134（134 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 STE STEARIC ACID × 42 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 PHO PHEOPHYTIN A × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；100mM MES pH6.5, 5mM CaCl2, 5% glycerol, 0.03% DDM
cryo-EM vitrification conditions Cryogen ETHANE；The sample-on-grid was illuminated with one and two flashes of light
Resolution 1.71 Å
9G6G Semi-active PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric
Chain U 1–134（134 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 3 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 LFA EICOSANE × 30 OEX CA-MN4-O5 CLUSTER × 1 LMT DODECYL-BETA-D-MALTOSIDE × 17 BCT BICARBONATE ION × 2 PLM PALMITIC ACID × 29 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 GOL GLYCEROL × 1 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 3 RRX (3R)-beta,beta-caroten-3-ol × 2 CA CALCIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å
9G6H active PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain U 1–134（134 aa）
Chain u 1–134（134 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 PLM PALMITIC ACID × 35 LFA EICOSANE × 24 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 GOL GLYCEROL × 2 LMT DODECYL-BETA-D-MALTOSIDE × 17 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 RRX (3R)-beta,beta-caroten-3-ol × 2 CA CALCIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å

59 other PDB entries and 64 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PSBU_THEEB
Isoform —
PDB entities 15
Chains and sequence ranges Author chain U； PDBConstruct 1–134； UniProt 1–134 Author chain u； PDBConstruct 1–134； UniProt 1–134

Cytochrome c-550

OrganismNot specified

UniProt P0A386

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 40 PDB declaration: 40-meric（40） Consistent with protein copy count Chain V； UniProt 1–163 Chain v； UniProt 1–163 Not recorded Photosystem II protein D1 1 × 2 （P0A444） Photosystem II CP47 reaction center protein × 2 （Q8DIQ1） Photosystem II CP43 reaction center protein × 2 （Q8DIF8） Photosystem II D2 protein × 2 （Q8CM25） Cytochrome b559 subunit alpha × 2 （Q8DIP0） Cytochrome b559 subunit beta × 2 （Q8DIN9） Photosystem II reaction center protein H × 2 （Q8DJ43） Photosystem II reaction center protein I × 2 （Q8DJZ6） Photosystem II reaction center protein J × 2 （P59087） Photosystem II reaction center protein K × 2 （Q9F1K9） Photosystem II reaction center protein L × 2 （Q8DIN8） Photosystem II reaction center protein M × 2 （Q8DHA7） Photosystem II manganese-stabilizing polypeptide × 2 （P0A431） Photosystem II reaction center protein T × 2 （Q8DIQ0） Photosystem II 12 kDa extrinsic protein × 2 （Q9F1L5） Photosystem II reaction center protein Ycf12 × 2 （Q8DJI1） Photosystem II reaction center X protein × 2 （Q9F1R6） Photosystem II reaction center protein Z × 2 （Q8DHJ2） Photosystem II protein Y × 2 （Q8DKM3） OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 UNL UNKNOWN LIGAND × 17 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION X-ray crystallization conditions：BATCH MODE；pH 7.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol. Resolution 3.00 Å R-free 0.303

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5KAF Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
11YO Single-conformation model re-refinement of 2F/S3-rich PSII intermediate structure at 2.09 Angstrom resolution Deposited 2026-03-18 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain V 1–163（163 aa）
Chain v 1–163（163 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 38 OEX CA-MN4-O5 CLUSTER × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 PHO PHEOPHYTIN A × 4 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.243
1MZ4 Crystal Structure of Cytochrome c550 from Thermosynechococcus elongatus Deposited 2002-10-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 27–163（137 aa）
Not recorded PO4 PHOSPHATE ION × 5 BCT BICARBONATE ION × 1 HEC HEME C × 1 GOL GLYCEROL × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；298 K；2 M dihydrogen phosphate, MES, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 1.80 Å R-free 0.202
1S5L Architecture of the photosynthetic oxygen evolving center Deposited 2004-01-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain V 27–163（137 aa）
Not recorded FE FE (III) ION × 1 BCT BICARBONATE ION × 2 OEC OXYGEN EVOLVING SYSTEM × 1 CLA CHLOROPHYLL A × 36 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMT DODECYL-BETA-D-MALTOSIDE × 1 BCR BETA-CAROTENE × 7 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pseudo-batch hanging drop；pH 7.5；291 K；100mM HEPES, 100mM (NH4)2SO4, PEG 4000, C12E8, trimethyl lead acetate, pH 7.5, pseudo-batch hanging drop, temperature 291K
Resolution 3.50 Å R-free 0.342
1S5L Architecture of the photosynthetic oxygen evolving center Deposited 2004-01-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain v 27–163（137 aa）
Not recorded FE FE (III) ION × 1 BCT BICARBONATE ION × 2 OEC OXYGEN EVOLVING SYSTEM × 1 CLA CHLOROPHYLL A × 36 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMT DODECYL-BETA-D-MALTOSIDE × 1 BCR BETA-CAROTENE × 7 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pseudo-batch hanging drop；pH 7.5；291 K；100mM HEPES, 100mM (NH4)2SO4, PEG 4000, C12E8, trimethyl lead acetate, pH 7.5, pseudo-batch hanging drop, temperature 291K
Resolution 3.50 Å R-free 0.342
2AXT Crystal Structure of Photosystem II from Thermosynechococcus elongatus Deposited 2005-09-06 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 36 PDB declaration: 36-meric
Chain V 27–163（137 aa）
Chain v 27–163（137 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PQ9 5-[(2E,6E,10E,14E,18E,22E)-3,7,11,15,19,23,27-HEPTAMETHYLOCTACOSA-2,6,10,14,18,22,26-HEPTAENYL]-2,3-DIMETHYLBENZO-1,4-QUINONE × 4 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 22 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LMT DODECYL-BETA-D-MALTOSIDE × 6 MGE (1S)-2-(ALPHA-L-ALLOPYRANOSYLOXY)-1-[(TRIDECANOYLOXY)METHYL]ETHYL PALMITATE × 12 UNL UNKNOWN LIGAND × 34 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG 2000, PIPES, magnesium chloride, pH 7.0, batch, temperature 298K
Resolution 3.00 Å R-free 0.286
3KZI Crystal Structure of Monomeric Form of Cyanobacterial Photosystem II Deposited 2009-12-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain V 27–163（137 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 11 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 LMT DODECYL-BETA-D-MALTOSIDE × 7 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 BCT BICARBONATE ION × 1 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；291 K；PEG 400, PIPES, MES, CaCl2, pH 7, batch, temperature 291.0K
Resolution 3.60 Å R-free 0.308
4FBY fs X-ray diffraction of Photosystem II Deposited 2012-05-23 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain V 27–163（137 aa）
Chain i 27–163（137 aa）
Not recorded CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 OEC OXYGEN EVOLVING SYSTEM × 2 FE2 FE (II) ION × 2 BCR BETA-CAROTENE × 24 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 CL CHLORIDE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；298 K；4% PEG 2000, 5 mM CaCl2, 100 mM PIPES, pH 7.0, batch, temperature 298K
Resolution 6.56 Å R-free 0.385
4IXQ RT fs X-ray diffraction of Photosystem II, dark state Deposited 2013-01-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain V 1–163（163 aa）
Chain v 1–163（163 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 LMT DODECYL-BETA-D-MALTOSIDE × 14 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.70 Å R-free 0.314
4IXR RT fs X-ray diffraction of Photosystem II, first illuminated state Deposited 2013-01-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain V 1–163（163 aa）
Chain v 1–163（163 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 LMT DODECYL-BETA-D-MALTOSIDE × 14 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.90 Å R-free 0.313
4PBU Serial Time-resolved crystallography of Photosystem II using a femtosecond X-ray laser The S1 state Deposited 2014-04-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain V 27–163（137 aa）
Chain v 27–163（137 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 CA CALCIUM ION × 7 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 5.00 Å R-free 0.262
4PJ0 Structure of T.elongatus Photosystem II, rows of dimers crystal packing Deposited 2014-05-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain V 1–163（163 aa）
Chain v 1–163（163 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE FE (III) ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SO4 SULFATE ION × 10 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 29 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；pH 7.5；293 K；Batch crystallisation: 2 mM Chl a equivalent of PSIIcc mixed 1:1 with: 0.1 M TRIS (pH 7.5), 0.1 M (NH4)2SO4, 15 - 18 % PEG 5000 MME, post-crystallisation dehydration
Resolution 2.44 Å R-free 0.256
4RVY Serial Time resolved crystallography of Photosystem II using a femtosecond X-ray laser. The S state after two flashes (S3) Deposited 2014-11-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain V 27–163（137 aa）
Chain v 27–163（137 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 CA CALCIUM ION × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions Free interface diffusion；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000 , Free interface diffusion, temperature 283K, temperature 283.0K
Resolution 5.50 Å R-free 0.291
4TNH RT XFEL structure of Photosystem II in the dark state at 4.9 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain V 1–163（163 aa）
Chain v 1–163（163 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.90 Å R-free 0.292
4TNI RT XFEL structure of Photosystem II 500 ms after the third illumination at 4.6 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain V 1–163（163 aa）
Chain v 1–163（163 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 2 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.60 Å R-free 0.284
4TNJ RT XFEL structure of Photosystem II 500 ms after the 2nd illumination (2F) at 4.5 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain V 1–163（163 aa）
Chain v 1–163（163 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.50 Å R-free 0.284
4TNK RT XFEL structure of Photosystem II 250 microsec after the third illumination at 5.2 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain V 1–163（163 aa）
Chain v 1–163（163 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.20 Å R-free 0.289
4V62 Crystal Structure of cyanobacterial Photosystem II Deposited 2008-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain AV 27–163（137 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 3 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 13 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 7 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 CL CHLORIDE ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 7 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG2000, PIPES, magnesium chloride, pH7.0, batch, temperature 298K
Resolution 2.90 Å R-free 0.292
4V62 Crystal Structure of cyanobacterial Photosystem II Deposited 2008-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain BV 27–163（137 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 3 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 11 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 7 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 CL CHLORIDE ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 7 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG2000, PIPES, magnesium chloride, pH7.0, batch, temperature 298K
Resolution 2.90 Å R-free 0.292
4V82 Crystal structure of cyanobacterial Photosystem II in complex with terbutryn Deposited 2010-11-30 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain AV 27–163（137 aa）
Chain BV 27–163（137 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 70 MST 2-T-BUTYLAMINO-4-ETHYLAMINO-6-METHYLTHIO-S-TRIAZINE × 2 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 LMT DODECYL-BETA-D-MALTOSIDE × 16 DMS DIMETHYL SULFOXIDE × 8 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；Pipes, CaCl2, DMSO, PEG 2000, pH 7.0, batch, temperature 298K
Resolution 3.20 Å R-free 0.299
5E79 Macromolecular diffractive imaging using imperfect crystals Deposited 2015-10-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain V 27–163（137 aa）
Chain v 27–163（137 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 CA CALCIUM ION × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；283.18 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 3.50 Å R-free 0.331
5E7C Macromolecular diffractive imaging using imperfect crystals - Bragg data Deposited 2015-10-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain V 27–163（137 aa）
Chain v 27–163（137 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 FE2 FE (II) ION × 2 CA CALCIUM ION × 6 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 4.50 Å R-free 0.275
5H2F Crystal structure of the PsbM-deletion mutant of photosystem II Deposited 2016-10-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain V 27–163（137 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 1 FE2 FE (II) ION × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 3 LMT DODECYL-BETA-D-MALTOSIDE × 6 UNL UNKNOWN LIGAND × 13 DMS DIMETHYL SULFOXIDE × 67 BCT BICARBONATE ION × 1 CA CALCIUM ION × 2 HTG heptyl 1-thio-beta-D-glucopyranoside × 9 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6.1；285 K；PEG1450, sodium chloride, calcium chloride, magnesium sulfate
Resolution 2.20 Å R-free 0.226
5H2F Crystal structure of the PsbM-deletion mutant of photosystem II Deposited 2016-10-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain v 27–163（137 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 1 FE2 FE (II) ION × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 5 LMT DODECYL-BETA-D-MALTOSIDE × 5 UNL UNKNOWN LIGAND × 14 DMS DIMETHYL SULFOXIDE × 66 BCT BICARBONATE ION × 1 CA CALCIUM ION × 3 HTG heptyl 1-thio-beta-D-glucopyranoside × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6.1；285 K；PEG1450, sodium chloride, calcium chloride, magnesium sulfate
Resolution 2.20 Å R-free 0.226
5KAI NH3-bound RT XFEL structure of Photosystem II 500 ms after the 2nd illumination (2F) at 2.8 A resolution Deposited 2016-06-01 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain V 1–163（163 aa）
Chain v 1–163（163 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 UNL UNKNOWN LIGAND × 23 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol
Resolution 2.80 Å R-free 0.300
5MX2 Photosystem II depleted of the Mn4CaO5 cluster at 2.55 A resolution Deposited 2017-01-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain V 1–163（163 aa）
Not recorded CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 11 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LFA EICOSANE × 19 FE FE (III) ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 4 BCT BICARBONATE ION × 1 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 7.5；291 K；equal volumes of buffer: 0.1 M Tris-HCl pH 7.5, 0.1 M (NH4)2SO4, 14-18 % PEG 5000 MME and protein: 2 mM chlorophyll equivalent of PSII in C12E8 detergent, post crystallisation treatment
Resolution 2.20 Å R-free 0.260
5MX2 Photosystem II depleted of the Mn4CaO5 cluster at 2.55 A resolution Deposited 2017-01-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain v 1–163（163 aa）
Not recorded CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 11 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LFA EICOSANE × 20 FE FE (III) ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 4 BCT BICARBONATE ION × 1 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 7.5；291 K；equal volumes of buffer: 0.1 M Tris-HCl pH 7.5, 0.1 M (NH4)2SO4, 14-18 % PEG 5000 MME and protein: 2 mM chlorophyll equivalent of PSII in C12E8 detergent, post crystallisation treatment
Resolution 2.20 Å R-free 0.260
5TIS Room temperature XFEL structure of the native, doubly-illuminated photosystem II complex Deposited 2016-10-03 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain V 1–163（163 aa）
Chain v 1–163（163 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 UNL UNKNOWN LIGAND × 33 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol
Resolution 2.25 Å R-free 0.231
5ZZN Crystal structure of photosystem II from an SQDG-deficient mutant of Thermosynechococcus elongatus Deposited 2018-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain V 27–163（137 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 5 UNL UNKNOWN LIGAND × 27 LMT DODECYL-BETA-D-MALTOSIDE × 9 DMS DIMETHYL SULFOXIDE × 48 CA CALCIUM ION × 3 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 HTG heptyl 1-thio-beta-D-glucopyranoside × 12 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 GOL GLYCEROL × 2 FE2 FE (II) ION × 1 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20MM NACL, 10MM CACL2, 40MM MGSO4, 20MM MES BUFFER, PH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPERATURE 285K
Resolution 2.10 Å R-free 0.197
5ZZN Crystal structure of photosystem II from an SQDG-deficient mutant of Thermosynechococcus elongatus Deposited 2018-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain v 27–163（137 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 1 CL CHLORIDE ION × 3 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 5 UNL UNKNOWN LIGAND × 25 LMT DODECYL-BETA-D-MALTOSIDE × 10 DMS DIMETHYL SULFOXIDE × 47 CA CALCIUM ION × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 HTG heptyl 1-thio-beta-D-glucopyranoside × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 GOL GLYCEROL × 3 FE2 FE (II) ION × 1 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20MM NACL, 10MM CACL2, 40MM MGSO4, 20MM MES BUFFER, PH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPERATURE 285K
Resolution 2.10 Å R-free 0.197
6DHE RT XFEL structure of the dark-stable state of Photosystem II (0F, S1-rich) at 2.05 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain V 27–163（137 aa）
Chain v 27–163（137 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 8CT (6'R,11cis,11'cis,13cis,15cis)-4',5'-didehydro-5',6'-dihydro-beta,beta-carotene × 12 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 BCR BETA-CAROTENE × 10 UNL UNKNOWN LIGAND × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.05 Å R-free 0.241
6DHF RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.08 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain V 27–163（137 aa）
Chain v 27–163（137 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 UNL UNKNOWN LIGAND × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.247
6DHG RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.5 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain V 27–163（137 aa）
Chain v 27–163（137 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.50 Å R-free 0.246
6DHH RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.2 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain V 27–163（137 aa）
Chain v 27–163（137 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.20 Å R-free 0.264
6DHO RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.07 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain V 27–163（137 aa）
Chain v 27–163（137 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.07 Å R-free 0.247
6DHP RT XFEL structure of the three-flash state of Photosystem II (3F, S0-rich) at 2.04 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain V 27–163（137 aa）
Chain v 27–163（137 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 UNL UNKNOWN LIGAND × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.04 Å R-free 0.248
6W1O RT XFEL structure of the dark-stable state of Photosystem II (0F, S1-rich) at 2.08 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain V 1–163（163 aa）
Chain v 1–163（163 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.240
6W1P RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.26 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain V 1–163（163 aa）
Chain v 1–163（163 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.26 Å R-free 0.241
6W1Q RT XFEL structure of Photosystem II 50 microseconds after the second illumination at 2.27 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain V 1–163（163 aa）
Chain v 1–163（163 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 BCR BETA-CAROTENE × 22 STE STEARIC ACID × 35 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 HEC HEME C × 4 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.27 Å R-free 0.249
6W1R RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.23 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain V 1–163（163 aa）
Chain v 1–163（163 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.23 Å R-free 0.235
6W1T RT XFEL structure of Photosystem II 250 microseconds after the second illumination at 2.01 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain V 1–163（163 aa）
Chain v 1–163（163 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.01 Å R-free 0.228
6W1U RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.09 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain V 1–163（163 aa）
Chain v 1–163（163 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
6W1V RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain V 1–163（163 aa）
Chain v 1–163（163 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 STE STEARIC ACID × 38 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 PHO PHEOPHYTIN A × 4 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.241
7RF1 RT XFEL structure of Photosystem II averaged across all S-states at 1.89 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain V 1–163（163 aa）
Chain v 1–163（163 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 STE STEARIC ACID × 55 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 1.89 Å R-free 0.214
7RF2 RT XFEL structure of dark-stable state of Photosystem II (0F, S1 rich) at 2.08 Angstrom Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain V 1–163（163 aa）
Chain v 1–163（163 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 33 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.238
7RF3 RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.26 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain V 1–163（163 aa）
Chain v 1–163（163 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.26 Å R-free 0.238
7RF4 RT XFEL structure of Photosystem II 50 microseconds after the second illumination at 2.27 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain V 1–163（163 aa）
Chain v 1–163（163 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 BCR BETA-CAROTENE × 22 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.27 Å R-free 0.244
7RF5 RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.23 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain V 1–163（163 aa）
Chain v 1–163（163 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.23 Å R-free 0.233
7RF6 RT XFEL structure of Photosystem II 250 microseconds after the second illumination at 2.01 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain V 1–163（163 aa）
Chain v 1–163（163 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.01 Å R-free 0.227
7RF7 RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.09 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain V 1–163（163 aa）
Chain v 1–163（163 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
7RF8 RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain V 1–163（163 aa）
Chain v 1–163（163 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
7YQ2 Crystal structure of photosystem II expressing psbA2 gene only Deposited 2022-08-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 39 PDB declaration: 39-meric
Chain V 1–163（163 aa）
Chain v 1–163（163 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 UNL UNKNOWN LIGAND × 77 LMT DODECYL-BETA-D-MALTOSIDE × 19 GOL GLYCEROL × 10 BCT BICARBONATE ION × 3 DMS DIMETHYL SULFOXIDE × 116 CA CALCIUM ION × 7 HTG heptyl 1-thio-beta-D-glucopyranoside × 31 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 9 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 MG MAGNESIUM ION × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20mM NaCl, 10mM CaCl2, 40mM MgSO4, 20mM Mes BUFFER, pH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPARATURE 285K
Resolution 1.90 Å R-free 0.184
7YQ7 Crystal structure of photosystem II expressing psbA3 gene only Deposited 2022-08-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 39 PDB declaration: 39-meric
Chain V 1–163（163 aa）
Chain v 1–163（163 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 UNL UNKNOWN LIGAND × 82 GOL GLYCEROL × 11 LMT DODECYL-BETA-D-MALTOSIDE × 18 BCT BICARBONATE ION × 4 DMS DIMETHYL SULFOXIDE × 71 CA CALCIUM ION × 5 HTG heptyl 1-thio-beta-D-glucopyranoside × 18 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 MG MAGNESIUM ION × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20mM NaCl, 10mM CaCl2, 40mM MgSO4, 20mM MES BUFFER pH 6.0, MICRO-BATCH METHOD UNDER OIL, TMPERATURE 285K
Resolution 1.90 Å R-free 0.177
8EZ5 RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2022-10-31 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain V 1–163（163 aa）
Chain v 1–163（163 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.234
8F4C RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain V 1–163（163 aa）
Chain v 1–163（163 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.224
8F4D RT XFEL structure of Photosystem II 50 microseconds after the third illumination at 2.15 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain V 1–163（163 aa）
Chain v 1–163（163 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.15 Å R-free 0.243
8F4E RT XFEL structure of Photosystem II 250 microseconds after the third illumination at 2.09 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain V 1–163（163 aa）
Chain v 1–163（163 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.233
8F4F RT XFEL structure of Photosystem II 500 microseconds after the third illumination at 2.03 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain V 1–163（163 aa）
Chain v 1–163（163 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.03 Å R-free 0.248
8F4G RT XFEL structure of Photosystem II 730 microseconds after the third illumination at 2.03 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain V 1–163（163 aa）
Chain v 1–163（163 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.03 Å R-free 0.223
8F4H RT XFEL structure of Photosystem II 1200 microseconds after the third illumination at 2.10 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain V 1–163（163 aa）
Chain v 1–163（163 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.10 Å R-free 0.232
8F4I RT XFEL structure of Photosystem II 2000 microseconds after the third illumination at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain V 1–163（163 aa）
Chain v 1–163（163 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.232
8F4J RT XFEL structure of Photosystem II 4000 microseconds after the third illumination at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain V 1–163（163 aa）
Chain v 1–163（163 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.226
8F4K RT XFEL structure of the three-flash state of Photosystem II (3F, S0-rich) at 2.16 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain V 1–163（163 aa）
Chain v 1–163（163 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.16 Å R-free 0.241
9EVX cryoEM structure of Photosystem II averaged across S2-S3 states at 1.71 Angstrom resolution Deposited 2024-04-02 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain V 1–163（163 aa）
Chain v 1–163（163 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 STE STEARIC ACID × 42 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 PHO PHEOPHYTIN A × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；100mM MES pH6.5, 5mM CaCl2, 5% glycerol, 0.03% DDM
cryo-EM vitrification conditions Cryogen ETHANE；The sample-on-grid was illuminated with one and two flashes of light
Resolution 1.71 Å
9G6G Semi-active PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric
Chain V 1–163（163 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 3 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 LFA EICOSANE × 30 OEX CA-MN4-O5 CLUSTER × 1 LMT DODECYL-BETA-D-MALTOSIDE × 17 BCT BICARBONATE ION × 2 PLM PALMITIC ACID × 29 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 GOL GLYCEROL × 1 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 3 RRX (3R)-beta,beta-caroten-3-ol × 2 CA CALCIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å
9G6H active PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain V 1–163（163 aa）
Chain v 1–163（163 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 PLM PALMITIC ACID × 35 LFA EICOSANE × 24 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 GOL GLYCEROL × 2 LMT DODECYL-BETA-D-MALTOSIDE × 17 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 RRX (3R)-beta,beta-caroten-3-ol × 2 CA CALCIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å

60 other PDB entries and 65 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name CY550_THEEB
Isoform —
PDB entities 16
Chains and sequence ranges Author chain V； PDBConstruct 1–163； UniProt 1–163 Author chain v； PDBConstruct 1–163； UniProt 1–163

Photosystem II reaction center protein Ycf12

OrganismNot specified

UniProt Q8DJI1

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 40 PDB declaration: 40-meric（40） Consistent with protein copy count Chain Y； UniProt 1–46 Chain y； UniProt 1–46 Not recorded Photosystem II protein D1 1 × 2 （P0A444） Photosystem II CP47 reaction center protein × 2 （Q8DIQ1） Photosystem II CP43 reaction center protein × 2 （Q8DIF8） Photosystem II D2 protein × 2 （Q8CM25） Cytochrome b559 subunit alpha × 2 （Q8DIP0） Cytochrome b559 subunit beta × 2 （Q8DIN9） Photosystem II reaction center protein H × 2 （Q8DJ43） Photosystem II reaction center protein I × 2 （Q8DJZ6） Photosystem II reaction center protein J × 2 （P59087） Photosystem II reaction center protein K × 2 （Q9F1K9） Photosystem II reaction center protein L × 2 （Q8DIN8） Photosystem II reaction center protein M × 2 （Q8DHA7） Photosystem II manganese-stabilizing polypeptide × 2 （P0A431） Photosystem II reaction center protein T × 2 （Q8DIQ0） Photosystem II 12 kDa extrinsic protein × 2 （Q9F1L5） Cytochrome c-550 × 2 （P0A386） Photosystem II reaction center X protein × 2 （Q9F1R6） Photosystem II reaction center protein Z × 2 （Q8DHJ2） Photosystem II protein Y × 2 （Q8DKM3） OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 UNL UNKNOWN LIGAND × 17 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION X-ray crystallization conditions：BATCH MODE；pH 7.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol. Resolution 3.00 Å R-free 0.303

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5KAF Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
11YO Single-conformation model re-refinement of 2F/S3-rich PSII intermediate structure at 2.09 Angstrom resolution Deposited 2026-03-18 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Y 1–46（46 aa）
Chain y 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 38 OEX CA-MN4-O5 CLUSTER × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 PHO PHEOPHYTIN A × 4 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.243
3KZI Crystal Structure of Monomeric Form of Cyanobacterial Photosystem II Deposited 2009-12-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain y 1–46（46 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 11 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 LMT DODECYL-BETA-D-MALTOSIDE × 7 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 BCT BICARBONATE ION × 1 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；291 K；PEG 400, PIPES, MES, CaCl2, pH 7, batch, temperature 291.0K
Resolution 3.60 Å R-free 0.308
4FBY fs X-ray diffraction of Photosystem II Deposited 2012-05-23 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain m 1–46（46 aa）
Chain y 1–46（46 aa）
Not recorded CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 OEC OXYGEN EVOLVING SYSTEM × 2 FE2 FE (II) ION × 2 BCR BETA-CAROTENE × 24 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 CL CHLORIDE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；298 K；4% PEG 2000, 5 mM CaCl2, 100 mM PIPES, pH 7.0, batch, temperature 298K
Resolution 6.56 Å R-free 0.385
4IXQ RT fs X-ray diffraction of Photosystem II, dark state Deposited 2013-01-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain g 1–46（46 aa）
Chain y 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 LMT DODECYL-BETA-D-MALTOSIDE × 14 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.70 Å R-free 0.314
4IXR RT fs X-ray diffraction of Photosystem II, first illuminated state Deposited 2013-01-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain g 1–46（46 aa）
Chain y 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 LMT DODECYL-BETA-D-MALTOSIDE × 14 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.90 Å R-free 0.313
4PBU Serial Time-resolved crystallography of Photosystem II using a femtosecond X-ray laser The S1 state Deposited 2014-04-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain Y 18–46（29 aa）
Chain y 18–46（29 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 CA CALCIUM ION × 7 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 5.00 Å R-free 0.262
4PJ0 Structure of T.elongatus Photosystem II, rows of dimers crystal packing Deposited 2014-05-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Y 1–46（46 aa）
Chain y 1–46（46 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE FE (III) ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SO4 SULFATE ION × 10 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 29 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；pH 7.5；293 K；Batch crystallisation: 2 mM Chl a equivalent of PSIIcc mixed 1:1 with: 0.1 M TRIS (pH 7.5), 0.1 M (NH4)2SO4, 15 - 18 % PEG 5000 MME, post-crystallisation dehydration
Resolution 2.44 Å R-free 0.256
4RVY Serial Time resolved crystallography of Photosystem II using a femtosecond X-ray laser. The S state after two flashes (S3) Deposited 2014-11-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain Y 18–46（29 aa）
Chain y 18–46（29 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 CA CALCIUM ION × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions Free interface diffusion；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000 , Free interface diffusion, temperature 283K, temperature 283.0K
Resolution 5.50 Å R-free 0.291
4TNH RT XFEL structure of Photosystem II in the dark state at 4.9 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain g 1–46（46 aa）
Chain y 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.90 Å R-free 0.292
4TNI RT XFEL structure of Photosystem II 500 ms after the third illumination at 4.6 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain g 1–46（46 aa）
Chain y 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 2 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.60 Å R-free 0.284
4TNJ RT XFEL structure of Photosystem II 500 ms after the 2nd illumination (2F) at 4.5 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain g 1–46（46 aa）
Chain y 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.50 Å R-free 0.284
4TNK RT XFEL structure of Photosystem II 250 microsec after the third illumination at 5.2 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain g 1–46（46 aa）
Chain y 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.20 Å R-free 0.289
4V62 Crystal Structure of cyanobacterial Photosystem II Deposited 2008-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain Ay 1–46（46 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 3 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 13 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 7 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 CL CHLORIDE ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 7 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG2000, PIPES, magnesium chloride, pH7.0, batch, temperature 298K
Resolution 2.90 Å R-free 0.292
4V62 Crystal Structure of cyanobacterial Photosystem II Deposited 2008-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain By 1–46（46 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 3 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 11 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 7 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 CL CHLORIDE ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 7 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG2000, PIPES, magnesium chloride, pH7.0, batch, temperature 298K
Resolution 2.90 Å R-free 0.292
4V82 Crystal structure of cyanobacterial Photosystem II in complex with terbutryn Deposited 2010-11-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Ay 1–46（46 aa）
Chain By 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 70 MST 2-T-BUTYLAMINO-4-ETHYLAMINO-6-METHYLTHIO-S-TRIAZINE × 2 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 LMT DODECYL-BETA-D-MALTOSIDE × 16 DMS DIMETHYL SULFOXIDE × 8 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；Pipes, CaCl2, DMSO, PEG 2000, pH 7.0, batch, temperature 298K
Resolution 3.20 Å R-free 0.299
5E79 Macromolecular diffractive imaging using imperfect crystals Deposited 2015-10-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain Y 18–46（29 aa）
Chain y 18–46（29 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 CA CALCIUM ION × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；283.18 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 3.50 Å R-free 0.331
5E7C Macromolecular diffractive imaging using imperfect crystals - Bragg data Deposited 2015-10-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain Y 18–46（29 aa）
Chain y 18–46（29 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 FE2 FE (II) ION × 2 CA CALCIUM ION × 6 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 4.50 Å R-free 0.275
5H2F Crystal structure of the PsbM-deletion mutant of photosystem II Deposited 2016-10-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain Y 18–46（29 aa） Fragment：UNP RESIDUES 18-46
Not recorded OEX CA-MN4-O5 CLUSTER × 1 FE2 FE (II) ION × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 3 LMT DODECYL-BETA-D-MALTOSIDE × 6 UNL UNKNOWN LIGAND × 13 DMS DIMETHYL SULFOXIDE × 67 BCT BICARBONATE ION × 1 CA CALCIUM ION × 2 HTG heptyl 1-thio-beta-D-glucopyranoside × 9 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6.1；285 K；PEG1450, sodium chloride, calcium chloride, magnesium sulfate
Resolution 2.20 Å R-free 0.226
5H2F Crystal structure of the PsbM-deletion mutant of photosystem II Deposited 2016-10-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain y 18–46（29 aa） Fragment：UNP RESIDUES 18-46
Not recorded OEX CA-MN4-O5 CLUSTER × 1 FE2 FE (II) ION × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 5 LMT DODECYL-BETA-D-MALTOSIDE × 5 UNL UNKNOWN LIGAND × 14 DMS DIMETHYL SULFOXIDE × 66 BCT BICARBONATE ION × 1 CA CALCIUM ION × 3 HTG heptyl 1-thio-beta-D-glucopyranoside × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6.1；285 K；PEG1450, sodium chloride, calcium chloride, magnesium sulfate
Resolution 2.20 Å R-free 0.226
5KAI NH3-bound RT XFEL structure of Photosystem II 500 ms after the 2nd illumination (2F) at 2.8 A resolution Deposited 2016-06-01 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Y 1–46（46 aa）
Chain y 1–46（46 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 UNL UNKNOWN LIGAND × 23 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol
Resolution 2.80 Å R-free 0.300
5MX2 Photosystem II depleted of the Mn4CaO5 cluster at 2.55 A resolution Deposited 2017-01-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain Y 1–46（46 aa）
Not recorded CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 11 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LFA EICOSANE × 19 FE FE (III) ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 4 BCT BICARBONATE ION × 1 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 7.5；291 K；equal volumes of buffer: 0.1 M Tris-HCl pH 7.5, 0.1 M (NH4)2SO4, 14-18 % PEG 5000 MME and protein: 2 mM chlorophyll equivalent of PSII in C12E8 detergent, post crystallisation treatment
Resolution 2.20 Å R-free 0.260
5MX2 Photosystem II depleted of the Mn4CaO5 cluster at 2.55 A resolution Deposited 2017-01-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain y 1–46（46 aa）
Not recorded CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 11 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LFA EICOSANE × 20 FE FE (III) ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 4 BCT BICARBONATE ION × 1 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 7.5；291 K；equal volumes of buffer: 0.1 M Tris-HCl pH 7.5, 0.1 M (NH4)2SO4, 14-18 % PEG 5000 MME and protein: 2 mM chlorophyll equivalent of PSII in C12E8 detergent, post crystallisation treatment
Resolution 2.20 Å R-free 0.260
5TIS Room temperature XFEL structure of the native, doubly-illuminated photosystem II complex Deposited 2016-10-03 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Y 1–46（46 aa）
Chain y 1–46（46 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 UNL UNKNOWN LIGAND × 33 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol
Resolution 2.25 Å R-free 0.231
5ZZN Crystal structure of photosystem II from an SQDG-deficient mutant of Thermosynechococcus elongatus Deposited 2018-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain Y 18–46（29 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 5 UNL UNKNOWN LIGAND × 27 LMT DODECYL-BETA-D-MALTOSIDE × 9 DMS DIMETHYL SULFOXIDE × 48 CA CALCIUM ION × 3 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 HTG heptyl 1-thio-beta-D-glucopyranoside × 12 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 GOL GLYCEROL × 2 FE2 FE (II) ION × 1 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20MM NACL, 10MM CACL2, 40MM MGSO4, 20MM MES BUFFER, PH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPERATURE 285K
Resolution 2.10 Å R-free 0.197
5ZZN Crystal structure of photosystem II from an SQDG-deficient mutant of Thermosynechococcus elongatus Deposited 2018-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain y 18–46（29 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 1 CL CHLORIDE ION × 3 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 5 UNL UNKNOWN LIGAND × 25 LMT DODECYL-BETA-D-MALTOSIDE × 10 DMS DIMETHYL SULFOXIDE × 47 CA CALCIUM ION × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 HTG heptyl 1-thio-beta-D-glucopyranoside × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 GOL GLYCEROL × 3 FE2 FE (II) ION × 1 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20MM NACL, 10MM CACL2, 40MM MGSO4, 20MM MES BUFFER, PH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPERATURE 285K
Resolution 2.10 Å R-free 0.197
6DHE RT XFEL structure of the dark-stable state of Photosystem II (0F, S1-rich) at 2.05 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Y 17–46（30 aa）
Chain y 17–46（30 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 8CT (6'R,11cis,11'cis,13cis,15cis)-4',5'-didehydro-5',6'-dihydro-beta,beta-carotene × 12 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 BCR BETA-CAROTENE × 10 UNL UNKNOWN LIGAND × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.05 Å R-free 0.241
6DHF RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.08 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Y 17–46（30 aa）
Chain y 17–46（30 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 UNL UNKNOWN LIGAND × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.247
6DHG RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.5 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Y 17–46（30 aa）
Chain y 17–46（30 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.50 Å R-free 0.246
6DHH RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.2 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Y 17–46（30 aa）
Chain y 17–46（30 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.20 Å R-free 0.264
6DHO RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.07 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Y 17–46（30 aa）
Chain y 17–46（30 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.07 Å R-free 0.247
6DHP RT XFEL structure of the three-flash state of Photosystem II (3F, S0-rich) at 2.04 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Y 17–46（30 aa）
Chain y 17–46（30 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 UNL UNKNOWN LIGAND × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.04 Å R-free 0.248
6W1O RT XFEL structure of the dark-stable state of Photosystem II (0F, S1-rich) at 2.08 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Y 1–46（46 aa）
Chain y 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.240
6W1P RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.26 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Y 1–46（46 aa）
Chain y 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.26 Å R-free 0.241
6W1Q RT XFEL structure of Photosystem II 50 microseconds after the second illumination at 2.27 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Y 1–46（46 aa）
Chain y 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 BCR BETA-CAROTENE × 22 STE STEARIC ACID × 35 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 HEC HEME C × 4 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.27 Å R-free 0.249
6W1R RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.23 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Y 1–46（46 aa）
Chain y 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.23 Å R-free 0.235
6W1T RT XFEL structure of Photosystem II 250 microseconds after the second illumination at 2.01 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Y 1–46（46 aa）
Chain y 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.01 Å R-free 0.228
6W1U RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.09 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Y 1–46（46 aa）
Chain y 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
6W1V RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Y 1–46（46 aa）
Chain y 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 STE STEARIC ACID × 38 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 PHO PHEOPHYTIN A × 4 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.241
7NHO Structure of PSII-M Deposited 2021-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain y 1–46（46 aa）
Not recorded FE FE (III) ION × 1 MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 BCT BICARBONATE ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.66 Å
7NHP Structure of PSII-I (PSII with Psb27, Psb28, and Psb34) Deposited 2021-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain y 1–46（46 aa）
Not recorded MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 FE FE (III) ION × 1 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.72 Å
7NHQ Structure of PSII-I prime (PSII with Psb28, and Psb34) Deposited 2021-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 17 PDB declaration: heptadecameric
Chain y 1–46（46 aa）
Not recorded FE FE (III) ION × 1 MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.68 Å
7RF1 RT XFEL structure of Photosystem II averaged across all S-states at 1.89 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Y 1–46（46 aa）
Chain y 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 STE STEARIC ACID × 55 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 1.89 Å R-free 0.214
7RF2 RT XFEL structure of dark-stable state of Photosystem II (0F, S1 rich) at 2.08 Angstrom Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Y 1–46（46 aa）
Chain y 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 33 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.238
7RF3 RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.26 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Y 1–46（46 aa）
Chain y 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.26 Å R-free 0.238
7RF4 RT XFEL structure of Photosystem II 50 microseconds after the second illumination at 2.27 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Y 1–46（46 aa）
Chain y 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 BCR BETA-CAROTENE × 22 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.27 Å R-free 0.244
7RF5 RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.23 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Y 1–46（46 aa）
Chain y 1–46（46 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.23 Å R-free 0.233
7RF6 RT XFEL structure of Photosystem II 250 microseconds after the second illumination at 2.01 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Y 1–46（46 aa）
Chain y 1–46（46 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.01 Å R-free 0.227
7RF7 RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.09 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Y 1–46（46 aa）
Chain y 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
7RF8 RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Y 1–46（46 aa）
Chain y 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
7YQ2 Crystal structure of photosystem II expressing psbA2 gene only Deposited 2022-08-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 39 PDB declaration: 39-meric
Chain Y 1–46（46 aa）
Chain y 1–46（46 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 UNL UNKNOWN LIGAND × 77 LMT DODECYL-BETA-D-MALTOSIDE × 19 GOL GLYCEROL × 10 BCT BICARBONATE ION × 3 DMS DIMETHYL SULFOXIDE × 116 CA CALCIUM ION × 7 HTG heptyl 1-thio-beta-D-glucopyranoside × 31 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 9 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 MG MAGNESIUM ION × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20mM NaCl, 10mM CaCl2, 40mM MgSO4, 20mM Mes BUFFER, pH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPARATURE 285K
Resolution 1.90 Å R-free 0.184
7YQ7 Crystal structure of photosystem II expressing psbA3 gene only Deposited 2022-08-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 39 PDB declaration: 39-meric
Chain Y 1–46（46 aa）
Chain y 1–46（46 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 UNL UNKNOWN LIGAND × 82 GOL GLYCEROL × 11 LMT DODECYL-BETA-D-MALTOSIDE × 18 BCT BICARBONATE ION × 4 DMS DIMETHYL SULFOXIDE × 71 CA CALCIUM ION × 5 HTG heptyl 1-thio-beta-D-glucopyranoside × 18 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 MG MAGNESIUM ION × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20mM NaCl, 10mM CaCl2, 40mM MgSO4, 20mM MES BUFFER pH 6.0, MICRO-BATCH METHOD UNDER OIL, TMPERATURE 285K
Resolution 1.90 Å R-free 0.177
8EZ5 RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2022-10-31 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Y 1–46（46 aa）
Chain y 1–46（46 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.234
8F4C RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Y 1–46（46 aa）
Chain y 1–46（46 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.224
8F4D RT XFEL structure of Photosystem II 50 microseconds after the third illumination at 2.15 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Y 1–46（46 aa）
Chain y 1–46（46 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.15 Å R-free 0.243
8F4E RT XFEL structure of Photosystem II 250 microseconds after the third illumination at 2.09 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Y 1–46（46 aa）
Chain y 1–46（46 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.233
8F4F RT XFEL structure of Photosystem II 500 microseconds after the third illumination at 2.03 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Y 1–46（46 aa）
Chain y 1–46（46 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.03 Å R-free 0.248
8F4G RT XFEL structure of Photosystem II 730 microseconds after the third illumination at 2.03 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Y 1–46（46 aa）
Chain y 1–46（46 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.03 Å R-free 0.223
8F4H RT XFEL structure of Photosystem II 1200 microseconds after the third illumination at 2.10 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Y 1–46（46 aa）
Chain y 1–46（46 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.10 Å R-free 0.232
8F4I RT XFEL structure of Photosystem II 2000 microseconds after the third illumination at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Y 1–46（46 aa）
Chain y 1–46（46 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.232
8F4J RT XFEL structure of Photosystem II 4000 microseconds after the third illumination at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Y 1–46（46 aa）
Chain y 1–46（46 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.226
8F4K RT XFEL structure of the three-flash state of Photosystem II (3F, S0-rich) at 2.16 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Y 1–46（46 aa）
Chain y 1–46（46 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.16 Å R-free 0.241
9EVX cryoEM structure of Photosystem II averaged across S2-S3 states at 1.71 Angstrom resolution Deposited 2024-04-02 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain Y 1–46（46 aa）
Chain y 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 STE STEARIC ACID × 42 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 PHO PHEOPHYTIN A × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；100mM MES pH6.5, 5mM CaCl2, 5% glycerol, 0.03% DDM
cryo-EM vitrification conditions Cryogen ETHANE；The sample-on-grid was illuminated with one and two flashes of light
Resolution 1.71 Å
9G6F Inactive PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain Y 1–46（46 aa）
Chain y 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LFA EICOSANE × 33 PLM PALMITIC ACID × 24 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 8 LMT DODECYL-BETA-D-MALTOSIDE × 13 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å
9G6G Semi-active PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric
Chain Y 1–46（46 aa）
Chain y 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 3 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 LFA EICOSANE × 30 OEX CA-MN4-O5 CLUSTER × 1 LMT DODECYL-BETA-D-MALTOSIDE × 17 BCT BICARBONATE ION × 2 PLM PALMITIC ACID × 29 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 GOL GLYCEROL × 1 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 3 RRX (3R)-beta,beta-caroten-3-ol × 2 CA CALCIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å
9G6H active PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain Y 1–46（46 aa）
Chain y 1–46（46 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 PLM PALMITIC ACID × 35 LFA EICOSANE × 24 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 GOL GLYCEROL × 2 LMT DODECYL-BETA-D-MALTOSIDE × 17 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 RRX (3R)-beta,beta-caroten-3-ol × 2 CA CALCIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å

61 other PDB entries and 65 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name YCF12_THEEB
Isoform —
PDB entities 17
Chains and sequence ranges Author chain Y； PDBConstruct 1–46； UniProt 1–46 Author chain y； PDBConstruct 1–46； UniProt 1–46

Photosystem II reaction center X protein

OrganismNot specified

UniProt Q9F1R6

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 40 PDB declaration: 40-meric（40） Consistent with protein copy count Chain X； UniProt 1–41 Chain x； UniProt 1–41 Not recorded Photosystem II protein D1 1 × 2 （P0A444） Photosystem II CP47 reaction center protein × 2 （Q8DIQ1） Photosystem II CP43 reaction center protein × 2 （Q8DIF8） Photosystem II D2 protein × 2 （Q8CM25） Cytochrome b559 subunit alpha × 2 （Q8DIP0） Cytochrome b559 subunit beta × 2 （Q8DIN9） Photosystem II reaction center protein H × 2 （Q8DJ43） Photosystem II reaction center protein I × 2 （Q8DJZ6） Photosystem II reaction center protein J × 2 （P59087） Photosystem II reaction center protein K × 2 （Q9F1K9） Photosystem II reaction center protein L × 2 （Q8DIN8） Photosystem II reaction center protein M × 2 （Q8DHA7） Photosystem II manganese-stabilizing polypeptide × 2 （P0A431） Photosystem II reaction center protein T × 2 （Q8DIQ0） Photosystem II 12 kDa extrinsic protein × 2 （Q9F1L5） Cytochrome c-550 × 2 （P0A386） Photosystem II reaction center protein Ycf12 × 2 （Q8DJI1） Photosystem II reaction center protein Z × 2 （Q8DHJ2） Photosystem II protein Y × 2 （Q8DKM3） OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 UNL UNKNOWN LIGAND × 17 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION X-ray crystallization conditions：BATCH MODE；pH 7.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol. Resolution 3.00 Å R-free 0.303

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5KAF Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
11YO Single-conformation model re-refinement of 2F/S3-rich PSII intermediate structure at 2.09 Angstrom resolution Deposited 2026-03-18 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain X 1–41（41 aa）
Chain x 1–41（41 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 38 OEX CA-MN4-O5 CLUSTER × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 PHO PHEOPHYTIN A × 4 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.243
1S5L Architecture of the photosynthetic oxygen evolving center Deposited 2004-01-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain X 2–41（40 aa）
Not recorded FE FE (III) ION × 1 BCT BICARBONATE ION × 2 OEC OXYGEN EVOLVING SYSTEM × 1 CLA CHLOROPHYLL A × 36 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMT DODECYL-BETA-D-MALTOSIDE × 1 BCR BETA-CAROTENE × 7 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pseudo-batch hanging drop；pH 7.5；291 K；100mM HEPES, 100mM (NH4)2SO4, PEG 4000, C12E8, trimethyl lead acetate, pH 7.5, pseudo-batch hanging drop, temperature 291K
Resolution 3.50 Å R-free 0.342
1S5L Architecture of the photosynthetic oxygen evolving center Deposited 2004-01-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain x 2–41（40 aa）
Not recorded FE FE (III) ION × 1 BCT BICARBONATE ION × 2 OEC OXYGEN EVOLVING SYSTEM × 1 CLA CHLOROPHYLL A × 36 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMT DODECYL-BETA-D-MALTOSIDE × 1 BCR BETA-CAROTENE × 7 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pseudo-batch hanging drop；pH 7.5；291 K；100mM HEPES, 100mM (NH4)2SO4, PEG 4000, C12E8, trimethyl lead acetate, pH 7.5, pseudo-batch hanging drop, temperature 291K
Resolution 3.50 Å R-free 0.342
3KZI Crystal Structure of Monomeric Form of Cyanobacterial Photosystem II Deposited 2009-12-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain X 2–41（40 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 11 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 LMT DODECYL-BETA-D-MALTOSIDE × 7 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 BCT BICARBONATE ION × 1 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；291 K；PEG 400, PIPES, MES, CaCl2, pH 7, batch, temperature 291.0K
Resolution 3.60 Å R-free 0.308
4FBY fs X-ray diffraction of Photosystem II Deposited 2012-05-23 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain X 2–41（40 aa）
Chain j 2–41（40 aa）
Not recorded CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 OEC OXYGEN EVOLVING SYSTEM × 2 FE2 FE (II) ION × 2 BCR BETA-CAROTENE × 24 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 CL CHLORIDE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；298 K；4% PEG 2000, 5 mM CaCl2, 100 mM PIPES, pH 7.0, batch, temperature 298K
Resolution 6.56 Å R-free 0.385
4IXQ RT fs X-ray diffraction of Photosystem II, dark state Deposited 2013-01-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain X 1–41（41 aa）
Chain x 1–41（41 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 LMT DODECYL-BETA-D-MALTOSIDE × 14 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.70 Å R-free 0.314
4IXR RT fs X-ray diffraction of Photosystem II, first illuminated state Deposited 2013-01-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain X 1–41（41 aa）
Chain x 1–41（41 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 LMT DODECYL-BETA-D-MALTOSIDE × 14 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.90 Å R-free 0.313
4PBU Serial Time-resolved crystallography of Photosystem II using a femtosecond X-ray laser The S1 state Deposited 2014-04-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain X 2–40（39 aa）
Chain x 2–40（39 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 CA CALCIUM ION × 7 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 5.00 Å R-free 0.262
4PJ0 Structure of T.elongatus Photosystem II, rows of dimers crystal packing Deposited 2014-05-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain X 1–41（41 aa）
Chain x 1–41（41 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE FE (III) ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SO4 SULFATE ION × 10 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 29 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；pH 7.5；293 K；Batch crystallisation: 2 mM Chl a equivalent of PSIIcc mixed 1:1 with: 0.1 M TRIS (pH 7.5), 0.1 M (NH4)2SO4, 15 - 18 % PEG 5000 MME, post-crystallisation dehydration
Resolution 2.44 Å R-free 0.256
4RVY Serial Time resolved crystallography of Photosystem II using a femtosecond X-ray laser. The S state after two flashes (S3) Deposited 2014-11-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain X 2–40（39 aa）
Chain x 2–40（39 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 CA CALCIUM ION × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions Free interface diffusion；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000 , Free interface diffusion, temperature 283K, temperature 283.0K
Resolution 5.50 Å R-free 0.291
4TNH RT XFEL structure of Photosystem II in the dark state at 4.9 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain X 1–41（41 aa）
Chain x 1–41（41 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.90 Å R-free 0.292
4TNI RT XFEL structure of Photosystem II 500 ms after the third illumination at 4.6 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain X 1–41（41 aa）
Chain x 1–41（41 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 2 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.60 Å R-free 0.284
4TNJ RT XFEL structure of Photosystem II 500 ms after the 2nd illumination (2F) at 4.5 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain X 1–41（41 aa）
Chain x 1–41（41 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.50 Å R-free 0.284
4TNK RT XFEL structure of Photosystem II 250 microsec after the third illumination at 5.2 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain X 1–41（41 aa）
Chain x 1–41（41 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.20 Å R-free 0.289
4V82 Crystal structure of cyanobacterial Photosystem II in complex with terbutryn Deposited 2010-11-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain AX 1–41（41 aa）
Chain BX 1–41（41 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 70 MST 2-T-BUTYLAMINO-4-ETHYLAMINO-6-METHYLTHIO-S-TRIAZINE × 2 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 LMT DODECYL-BETA-D-MALTOSIDE × 16 DMS DIMETHYL SULFOXIDE × 8 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；Pipes, CaCl2, DMSO, PEG 2000, pH 7.0, batch, temperature 298K
Resolution 3.20 Å R-free 0.299
5E79 Macromolecular diffractive imaging using imperfect crystals Deposited 2015-10-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain X 2–40（39 aa）
Chain x 2–40（39 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 CA CALCIUM ION × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；283.18 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 3.50 Å R-free 0.331
5E7C Macromolecular diffractive imaging using imperfect crystals - Bragg data Deposited 2015-10-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain X 2–40（39 aa）
Chain x 2–40（39 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 FE2 FE (II) ION × 2 CA CALCIUM ION × 6 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 4.50 Å R-free 0.275
5H2F Crystal structure of the PsbM-deletion mutant of photosystem II Deposited 2016-10-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain X 2–38（37 aa） Fragment：UNP RESIDUES 2-38
Not recorded OEX CA-MN4-O5 CLUSTER × 1 FE2 FE (II) ION × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 3 LMT DODECYL-BETA-D-MALTOSIDE × 6 UNL UNKNOWN LIGAND × 13 DMS DIMETHYL SULFOXIDE × 67 BCT BICARBONATE ION × 1 CA CALCIUM ION × 2 HTG heptyl 1-thio-beta-D-glucopyranoside × 9 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6.1；285 K；PEG1450, sodium chloride, calcium chloride, magnesium sulfate
Resolution 2.20 Å R-free 0.226
5H2F Crystal structure of the PsbM-deletion mutant of photosystem II Deposited 2016-10-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain x 2–38（37 aa） Fragment：UNP RESIDUES 2-38
Not recorded OEX CA-MN4-O5 CLUSTER × 1 FE2 FE (II) ION × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 5 LMT DODECYL-BETA-D-MALTOSIDE × 5 UNL UNKNOWN LIGAND × 14 DMS DIMETHYL SULFOXIDE × 66 BCT BICARBONATE ION × 1 CA CALCIUM ION × 3 HTG heptyl 1-thio-beta-D-glucopyranoside × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6.1；285 K；PEG1450, sodium chloride, calcium chloride, magnesium sulfate
Resolution 2.20 Å R-free 0.226
5KAI NH3-bound RT XFEL structure of Photosystem II 500 ms after the 2nd illumination (2F) at 2.8 A resolution Deposited 2016-06-01 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain X 1–41（41 aa）
Chain x 1–41（41 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 UNL UNKNOWN LIGAND × 23 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol
Resolution 2.80 Å R-free 0.300
5MX2 Photosystem II depleted of the Mn4CaO5 cluster at 2.55 A resolution Deposited 2017-01-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain X 1–41（41 aa）
Not recorded CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 11 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LFA EICOSANE × 19 FE FE (III) ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 4 BCT BICARBONATE ION × 1 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 7.5；291 K；equal volumes of buffer: 0.1 M Tris-HCl pH 7.5, 0.1 M (NH4)2SO4, 14-18 % PEG 5000 MME and protein: 2 mM chlorophyll equivalent of PSII in C12E8 detergent, post crystallisation treatment
Resolution 2.20 Å R-free 0.260
5MX2 Photosystem II depleted of the Mn4CaO5 cluster at 2.55 A resolution Deposited 2017-01-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain x 1–41（41 aa）
Not recorded CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 11 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LFA EICOSANE × 20 FE FE (III) ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 4 BCT BICARBONATE ION × 1 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 7.5；291 K；equal volumes of buffer: 0.1 M Tris-HCl pH 7.5, 0.1 M (NH4)2SO4, 14-18 % PEG 5000 MME and protein: 2 mM chlorophyll equivalent of PSII in C12E8 detergent, post crystallisation treatment
Resolution 2.20 Å R-free 0.260
5TIS Room temperature XFEL structure of the native, doubly-illuminated photosystem II complex Deposited 2016-10-03 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain X 1–41（41 aa）
Chain x 1–41（41 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 UNL UNKNOWN LIGAND × 33 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol
Resolution 2.25 Å R-free 0.231
5ZZN Crystal structure of photosystem II from an SQDG-deficient mutant of Thermosynechococcus elongatus Deposited 2018-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain X 2–40（39 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 5 UNL UNKNOWN LIGAND × 27 LMT DODECYL-BETA-D-MALTOSIDE × 9 DMS DIMETHYL SULFOXIDE × 48 CA CALCIUM ION × 3 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 HTG heptyl 1-thio-beta-D-glucopyranoside × 12 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 GOL GLYCEROL × 2 FE2 FE (II) ION × 1 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20MM NACL, 10MM CACL2, 40MM MGSO4, 20MM MES BUFFER, PH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPERATURE 285K
Resolution 2.10 Å R-free 0.197
5ZZN Crystal structure of photosystem II from an SQDG-deficient mutant of Thermosynechococcus elongatus Deposited 2018-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain x 2–40（39 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 1 CL CHLORIDE ION × 3 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 5 UNL UNKNOWN LIGAND × 25 LMT DODECYL-BETA-D-MALTOSIDE × 10 DMS DIMETHYL SULFOXIDE × 47 CA CALCIUM ION × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 HTG heptyl 1-thio-beta-D-glucopyranoside × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 GOL GLYCEROL × 3 FE2 FE (II) ION × 1 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20MM NACL, 10MM CACL2, 40MM MGSO4, 20MM MES BUFFER, PH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPERATURE 285K
Resolution 2.10 Å R-free 0.197
6DHE RT XFEL structure of the dark-stable state of Photosystem II (0F, S1-rich) at 2.05 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain X 2–39（38 aa）
Chain x 2–39（38 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 8CT (6'R,11cis,11'cis,13cis,15cis)-4',5'-didehydro-5',6'-dihydro-beta,beta-carotene × 12 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 BCR BETA-CAROTENE × 10 UNL UNKNOWN LIGAND × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.05 Å R-free 0.241
6DHF RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.08 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain X 2–39（38 aa）
Chain x 2–39（38 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 UNL UNKNOWN LIGAND × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.247
6DHG RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.5 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain X 2–39（38 aa）
Chain x 2–39（38 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.50 Å R-free 0.246
6DHH RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.2 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain X 2–39（38 aa）
Chain x 2–39（38 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.20 Å R-free 0.264
6DHO RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.07 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain X 2–39（38 aa）
Chain x 2–39（38 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.07 Å R-free 0.247
6DHP RT XFEL structure of the three-flash state of Photosystem II (3F, S0-rich) at 2.04 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain X 2–39（38 aa）
Chain x 2–39（38 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 UNL UNKNOWN LIGAND × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.04 Å R-free 0.248
6W1O RT XFEL structure of the dark-stable state of Photosystem II (0F, S1-rich) at 2.08 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain X 1–41（41 aa）
Chain x 1–41（41 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.240
6W1P RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.26 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain X 1–41（41 aa）
Chain x 1–41（41 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.26 Å R-free 0.241
6W1Q RT XFEL structure of Photosystem II 50 microseconds after the second illumination at 2.27 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain X 1–41（41 aa）
Chain x 1–41（41 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 BCR BETA-CAROTENE × 22 STE STEARIC ACID × 35 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 HEC HEME C × 4 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.27 Å R-free 0.249
6W1R RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.23 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain X 1–41（41 aa）
Chain x 1–41（41 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.23 Å R-free 0.235
6W1T RT XFEL structure of Photosystem II 250 microseconds after the second illumination at 2.01 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain X 1–41（41 aa）
Chain x 1–41（41 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.01 Å R-free 0.228
6W1U RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.09 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain X 1–41（41 aa）
Chain x 1–41（41 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
6W1V RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain X 1–41（41 aa）
Chain x 1–41（41 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 STE STEARIC ACID × 38 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 PHO PHEOPHYTIN A × 4 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.241
7NHO Structure of PSII-M Deposited 2021-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain X 1–41（41 aa）
Not recorded FE FE (III) ION × 1 MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 BCT BICARBONATE ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.66 Å
7NHP Structure of PSII-I (PSII with Psb27, Psb28, and Psb34) Deposited 2021-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain X 1–41（41 aa）
Not recorded MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 FE FE (III) ION × 1 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.72 Å
7NHQ Structure of PSII-I prime (PSII with Psb28, and Psb34) Deposited 2021-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 17 PDB declaration: heptadecameric
Chain X 1–41（41 aa）
Not recorded FE FE (III) ION × 1 MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.68 Å
7RF1 RT XFEL structure of Photosystem II averaged across all S-states at 1.89 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain X 1–41（41 aa）
Chain x 1–41（41 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 STE STEARIC ACID × 55 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 1.89 Å R-free 0.214
7RF2 RT XFEL structure of dark-stable state of Photosystem II (0F, S1 rich) at 2.08 Angstrom Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain X 1–41（41 aa）
Chain x 1–41（41 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 33 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.238
7RF3 RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.26 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain X 1–41（41 aa）
Chain x 1–41（41 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.26 Å R-free 0.238
7RF4 RT XFEL structure of Photosystem II 50 microseconds after the second illumination at 2.27 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain X 1–41（41 aa）
Chain x 1–41（41 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 BCR BETA-CAROTENE × 22 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.27 Å R-free 0.244
7RF5 RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.23 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain X 1–41（41 aa）
Chain x 1–41（41 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.23 Å R-free 0.233
7RF6 RT XFEL structure of Photosystem II 250 microseconds after the second illumination at 2.01 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain X 1–41（41 aa）
Chain x 1–41（41 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.01 Å R-free 0.227
7RF7 RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.09 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain X 1–41（41 aa）
Chain x 1–41（41 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
7RF8 RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain X 1–41（41 aa）
Chain x 1–41（41 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
7YQ2 Crystal structure of photosystem II expressing psbA2 gene only Deposited 2022-08-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 39 PDB declaration: 39-meric
Chain X 1–41（41 aa）
Chain x 1–41（41 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 UNL UNKNOWN LIGAND × 77 LMT DODECYL-BETA-D-MALTOSIDE × 19 GOL GLYCEROL × 10 BCT BICARBONATE ION × 3 DMS DIMETHYL SULFOXIDE × 116 CA CALCIUM ION × 7 HTG heptyl 1-thio-beta-D-glucopyranoside × 31 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 9 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 MG MAGNESIUM ION × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20mM NaCl, 10mM CaCl2, 40mM MgSO4, 20mM Mes BUFFER, pH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPARATURE 285K
Resolution 1.90 Å R-free 0.184
7YQ7 Crystal structure of photosystem II expressing psbA3 gene only Deposited 2022-08-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 39 PDB declaration: 39-meric
Chain X 1–41（41 aa）
Chain x 1–41（41 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 UNL UNKNOWN LIGAND × 82 GOL GLYCEROL × 11 LMT DODECYL-BETA-D-MALTOSIDE × 18 BCT BICARBONATE ION × 4 DMS DIMETHYL SULFOXIDE × 71 CA CALCIUM ION × 5 HTG heptyl 1-thio-beta-D-glucopyranoside × 18 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 MG MAGNESIUM ION × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20mM NaCl, 10mM CaCl2, 40mM MgSO4, 20mM MES BUFFER pH 6.0, MICRO-BATCH METHOD UNDER OIL, TMPERATURE 285K
Resolution 1.90 Å R-free 0.177
8EZ5 RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2022-10-31 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain X 1–41（41 aa）
Chain x 1–41（41 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.234
8F4C RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain X 1–41（41 aa）
Chain x 1–41（41 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.224
8F4D RT XFEL structure of Photosystem II 50 microseconds after the third illumination at 2.15 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain X 1–41（41 aa）
Chain x 1–41（41 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.15 Å R-free 0.243
8F4E RT XFEL structure of Photosystem II 250 microseconds after the third illumination at 2.09 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain X 1–41（41 aa）
Chain x 1–41（41 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.233
8F4F RT XFEL structure of Photosystem II 500 microseconds after the third illumination at 2.03 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain X 1–41（41 aa）
Chain x 1–41（41 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.03 Å R-free 0.248
8F4G RT XFEL structure of Photosystem II 730 microseconds after the third illumination at 2.03 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain X 1–41（41 aa）
Chain x 1–41（41 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.03 Å R-free 0.223
8F4H RT XFEL structure of Photosystem II 1200 microseconds after the third illumination at 2.10 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain X 1–41（41 aa）
Chain x 1–41（41 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.10 Å R-free 0.232
8F4I RT XFEL structure of Photosystem II 2000 microseconds after the third illumination at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain X 1–41（41 aa）
Chain x 1–41（41 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.232
8F4J RT XFEL structure of Photosystem II 4000 microseconds after the third illumination at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain X 1–41（41 aa）
Chain x 1–41（41 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.226
8F4K RT XFEL structure of the three-flash state of Photosystem II (3F, S0-rich) at 2.16 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain X 1–41（41 aa）
Chain x 1–41（41 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.16 Å R-free 0.241
9EVX cryoEM structure of Photosystem II averaged across S2-S3 states at 1.71 Angstrom resolution Deposited 2024-04-02 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain X 1–41（41 aa）
Chain x 1–41（41 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 STE STEARIC ACID × 42 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 PHO PHEOPHYTIN A × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；100mM MES pH6.5, 5mM CaCl2, 5% glycerol, 0.03% DDM
cryo-EM vitrification conditions Cryogen ETHANE；The sample-on-grid was illuminated with one and two flashes of light
Resolution 1.71 Å
9G6F Inactive PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain X 1–41（41 aa）
Chain x 1–41（41 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LFA EICOSANE × 33 PLM PALMITIC ACID × 24 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 8 LMT DODECYL-BETA-D-MALTOSIDE × 13 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å
9G6G Semi-active PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric
Chain X 1–41（41 aa）
Chain x 1–41（41 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 3 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 LFA EICOSANE × 30 OEX CA-MN4-O5 CLUSTER × 1 LMT DODECYL-BETA-D-MALTOSIDE × 17 BCT BICARBONATE ION × 2 PLM PALMITIC ACID × 29 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 GOL GLYCEROL × 1 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 3 RRX (3R)-beta,beta-caroten-3-ol × 2 CA CALCIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å
9G6H active PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain X 1–41（41 aa）
Chain x 1–41（41 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 PLM PALMITIC ACID × 35 LFA EICOSANE × 24 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 GOL GLYCEROL × 2 LMT DODECYL-BETA-D-MALTOSIDE × 17 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 RRX (3R)-beta,beta-caroten-3-ol × 2 CA CALCIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å

61 other PDB entries and 65 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PSBX_THEEB
Isoform —
PDB entities 18
Chains and sequence ranges Author chain X； PDBConstruct 1–41； UniProt 1–41 Author chain x； PDBConstruct 1–41； UniProt 1–41

Photosystem II reaction center protein Z

OrganismNot specified

UniProt Q8DHJ2

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 40 PDB declaration: 40-meric（40） Consistent with protein copy count Chain Z； UniProt 1–62 Chain z； UniProt 1–62 Not recorded Photosystem II protein D1 1 × 2 （P0A444） Photosystem II CP47 reaction center protein × 2 （Q8DIQ1） Photosystem II CP43 reaction center protein × 2 （Q8DIF8） Photosystem II D2 protein × 2 （Q8CM25） Cytochrome b559 subunit alpha × 2 （Q8DIP0） Cytochrome b559 subunit beta × 2 （Q8DIN9） Photosystem II reaction center protein H × 2 （Q8DJ43） Photosystem II reaction center protein I × 2 （Q8DJZ6） Photosystem II reaction center protein J × 2 （P59087） Photosystem II reaction center protein K × 2 （Q9F1K9） Photosystem II reaction center protein L × 2 （Q8DIN8） Photosystem II reaction center protein M × 2 （Q8DHA7） Photosystem II manganese-stabilizing polypeptide × 2 （P0A431） Photosystem II reaction center protein T × 2 （Q8DIQ0） Photosystem II 12 kDa extrinsic protein × 2 （Q9F1L5） Cytochrome c-550 × 2 （P0A386） Photosystem II reaction center protein Ycf12 × 2 （Q8DJI1） Photosystem II reaction center X protein × 2 （Q9F1R6） Photosystem II protein Y × 2 （Q8DKM3） OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 UNL UNKNOWN LIGAND × 17 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION X-ray crystallization conditions：BATCH MODE；pH 7.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol. Resolution 3.00 Å R-free 0.303

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5KAF Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
11YO Single-conformation model re-refinement of 2F/S3-rich PSII intermediate structure at 2.09 Angstrom resolution Deposited 2026-03-18 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 38 OEX CA-MN4-O5 CLUSTER × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 PHO PHEOPHYTIN A × 4 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.243
1S5L Architecture of the photosynthetic oxygen evolving center Deposited 2004-01-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain Z 1–62（62 aa）
Not recorded FE FE (III) ION × 1 BCT BICARBONATE ION × 2 OEC OXYGEN EVOLVING SYSTEM × 1 CLA CHLOROPHYLL A × 36 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMT DODECYL-BETA-D-MALTOSIDE × 1 BCR BETA-CAROTENE × 7 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pseudo-batch hanging drop；pH 7.5；291 K；100mM HEPES, 100mM (NH4)2SO4, PEG 4000, C12E8, trimethyl lead acetate, pH 7.5, pseudo-batch hanging drop, temperature 291K
Resolution 3.50 Å R-free 0.342
1S5L Architecture of the photosynthetic oxygen evolving center Deposited 2004-01-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain z 1–62（62 aa）
Not recorded FE FE (III) ION × 1 BCT BICARBONATE ION × 2 OEC OXYGEN EVOLVING SYSTEM × 1 CLA CHLOROPHYLL A × 36 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMT DODECYL-BETA-D-MALTOSIDE × 1 BCR BETA-CAROTENE × 7 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pseudo-batch hanging drop；pH 7.5；291 K；100mM HEPES, 100mM (NH4)2SO4, PEG 4000, C12E8, trimethyl lead acetate, pH 7.5, pseudo-batch hanging drop, temperature 291K
Resolution 3.50 Å R-free 0.342
2AXT Crystal Structure of Photosystem II from Thermosynechococcus elongatus Deposited 2005-09-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 36 PDB declaration: 36-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PQ9 5-[(2E,6E,10E,14E,18E,22E)-3,7,11,15,19,23,27-HEPTAMETHYLOCTACOSA-2,6,10,14,18,22,26-HEPTAENYL]-2,3-DIMETHYLBENZO-1,4-QUINONE × 4 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 22 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LMT DODECYL-BETA-D-MALTOSIDE × 6 MGE (1S)-2-(ALPHA-L-ALLOPYRANOSYLOXY)-1-[(TRIDECANOYLOXY)METHYL]ETHYL PALMITATE × 12 UNL UNKNOWN LIGAND × 34 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG 2000, PIPES, magnesium chloride, pH 7.0, batch, temperature 298K
Resolution 3.00 Å R-free 0.286
3KZI Crystal Structure of Monomeric Form of Cyanobacterial Photosystem II Deposited 2009-12-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain Z 1–62（62 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 11 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 LMT DODECYL-BETA-D-MALTOSIDE × 7 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 BCT BICARBONATE ION × 1 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；291 K；PEG 400, PIPES, MES, CaCl2, pH 7, batch, temperature 291.0K
Resolution 3.60 Å R-free 0.308
4FBY fs X-ray diffraction of Photosystem II Deposited 2012-05-23 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Z 1–62（62 aa）
Chain l 1–62（62 aa）
Not recorded CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 OEC OXYGEN EVOLVING SYSTEM × 2 FE2 FE (II) ION × 2 BCR BETA-CAROTENE × 24 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 CL CHLORIDE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；298 K；4% PEG 2000, 5 mM CaCl2, 100 mM PIPES, pH 7.0, batch, temperature 298K
Resolution 6.56 Å R-free 0.385
4IXQ RT fs X-ray diffraction of Photosystem II, dark state Deposited 2013-01-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 LMT DODECYL-BETA-D-MALTOSIDE × 14 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.70 Å R-free 0.314
4IXR RT fs X-ray diffraction of Photosystem II, first illuminated state Deposited 2013-01-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 LMT DODECYL-BETA-D-MALTOSIDE × 14 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.90 Å R-free 0.313
4PBU Serial Time-resolved crystallography of Photosystem II using a femtosecond X-ray laser The S1 state Deposited 2014-04-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 CA CALCIUM ION × 7 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 5.00 Å R-free 0.262
4PJ0 Structure of T.elongatus Photosystem II, rows of dimers crystal packing Deposited 2014-05-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE FE (III) ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SO4 SULFATE ION × 10 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 29 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；pH 7.5；293 K；Batch crystallisation: 2 mM Chl a equivalent of PSIIcc mixed 1:1 with: 0.1 M TRIS (pH 7.5), 0.1 M (NH4)2SO4, 15 - 18 % PEG 5000 MME, post-crystallisation dehydration
Resolution 2.44 Å R-free 0.256
4RVY Serial Time resolved crystallography of Photosystem II using a femtosecond X-ray laser. The S state after two flashes (S3) Deposited 2014-11-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 CA CALCIUM ION × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions Free interface diffusion；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000 , Free interface diffusion, temperature 283K, temperature 283.0K
Resolution 5.50 Å R-free 0.291
4TNH RT XFEL structure of Photosystem II in the dark state at 4.9 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.90 Å R-free 0.292
4TNI RT XFEL structure of Photosystem II 500 ms after the third illumination at 4.6 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 2 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.60 Å R-free 0.284
4TNJ RT XFEL structure of Photosystem II 500 ms after the 2nd illumination (2F) at 4.5 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 4.50 Å R-free 0.284
4TNK RT XFEL structure of Photosystem II 250 microsec after the third illumination at 5.2 A resolution Deposited 2014-06-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
Resolution 5.20 Å R-free 0.289
4V62 Crystal Structure of cyanobacterial Photosystem II Deposited 2008-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain AZ 1–62（62 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 3 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 13 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 7 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 CL CHLORIDE ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 7 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG2000, PIPES, magnesium chloride, pH7.0, batch, temperature 298K
Resolution 2.90 Å R-free 0.292
4V62 Crystal Structure of cyanobacterial Photosystem II Deposited 2008-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain BZ 1–62（62 aa）
Not recorded FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 3 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 11 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 7 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 CL CHLORIDE ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 7 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；PEG2000, PIPES, magnesium chloride, pH7.0, batch, temperature 298K
Resolution 2.90 Å R-free 0.292
4V82 Crystal structure of cyanobacterial Photosystem II in complex with terbutryn Deposited 2010-11-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain AZ 1–62（62 aa）
Chain BZ 1–62（62 aa）
Not recorded FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 70 MST 2-T-BUTYLAMINO-4-ETHYLAMINO-6-METHYLTHIO-S-TRIAZINE × 2 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 LMT DODECYL-BETA-D-MALTOSIDE × 16 DMS DIMETHYL SULFOXIDE × 8 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions batch；pH 7；298 K；Pipes, CaCl2, DMSO, PEG 2000, pH 7.0, batch, temperature 298K
Resolution 3.20 Å R-free 0.299
5E79 Macromolecular diffractive imaging using imperfect crystals Deposited 2015-10-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 CA CALCIUM ION × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；283.18 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 3.50 Å R-free 0.331
5E7C Macromolecular diffractive imaging using imperfect crystals - Bragg data Deposited 2015-10-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 FE2 FE (II) ION × 2 CA CALCIUM ION × 6 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
Resolution 4.50 Å R-free 0.275
5H2F Crystal structure of the PsbM-deletion mutant of photosystem II Deposited 2016-10-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain Z 1–62（62 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 1 FE2 FE (II) ION × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 3 LMT DODECYL-BETA-D-MALTOSIDE × 6 UNL UNKNOWN LIGAND × 13 DMS DIMETHYL SULFOXIDE × 67 BCT BICARBONATE ION × 1 CA CALCIUM ION × 2 HTG heptyl 1-thio-beta-D-glucopyranoside × 9 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6.1；285 K；PEG1450, sodium chloride, calcium chloride, magnesium sulfate
Resolution 2.20 Å R-free 0.226
5H2F Crystal structure of the PsbM-deletion mutant of photosystem II Deposited 2016-10-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain z 1–62（62 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 1 FE2 FE (II) ION × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 5 LMT DODECYL-BETA-D-MALTOSIDE × 5 UNL UNKNOWN LIGAND × 14 DMS DIMETHYL SULFOXIDE × 66 BCT BICARBONATE ION × 1 CA CALCIUM ION × 3 HTG heptyl 1-thio-beta-D-glucopyranoside × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6.1；285 K；PEG1450, sodium chloride, calcium chloride, magnesium sulfate
Resolution 2.20 Å R-free 0.226
5KAI NH3-bound RT XFEL structure of Photosystem II 500 ms after the 2nd illumination (2F) at 2.8 A resolution Deposited 2016-06-01 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 UNL UNKNOWN LIGAND × 23 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol
Resolution 2.80 Å R-free 0.300
5MX2 Photosystem II depleted of the Mn4CaO5 cluster at 2.55 A resolution Deposited 2017-01-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain Z 1–62（62 aa）
Not recorded CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 11 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LFA EICOSANE × 19 FE FE (III) ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 4 BCT BICARBONATE ION × 1 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 7.5；291 K；equal volumes of buffer: 0.1 M Tris-HCl pH 7.5, 0.1 M (NH4)2SO4, 14-18 % PEG 5000 MME and protein: 2 mM chlorophyll equivalent of PSII in C12E8 detergent, post crystallisation treatment
Resolution 2.20 Å R-free 0.260
5MX2 Photosystem II depleted of the Mn4CaO5 cluster at 2.55 A resolution Deposited 2017-01-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain z 1–62（62 aa）
Not recorded CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 11 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LFA EICOSANE × 20 FE FE (III) ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 4 BCT BICARBONATE ION × 1 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 7.5；291 K；equal volumes of buffer: 0.1 M Tris-HCl pH 7.5, 0.1 M (NH4)2SO4, 14-18 % PEG 5000 MME and protein: 2 mM chlorophyll equivalent of PSII in C12E8 detergent, post crystallisation treatment
Resolution 2.20 Å R-free 0.260
5TIS Room temperature XFEL structure of the native, doubly-illuminated photosystem II complex Deposited 2016-10-03 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 UNL UNKNOWN LIGAND × 33 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol
Resolution 2.25 Å R-free 0.231
5ZZN Crystal structure of photosystem II from an SQDG-deficient mutant of Thermosynechococcus elongatus Deposited 2018-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain Z 1–62（62 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 5 UNL UNKNOWN LIGAND × 27 LMT DODECYL-BETA-D-MALTOSIDE × 9 DMS DIMETHYL SULFOXIDE × 48 CA CALCIUM ION × 3 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 HTG heptyl 1-thio-beta-D-glucopyranoside × 12 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 GOL GLYCEROL × 2 FE2 FE (II) ION × 1 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20MM NACL, 10MM CACL2, 40MM MGSO4, 20MM MES BUFFER, PH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPERATURE 285K
Resolution 2.10 Å R-free 0.197
5ZZN Crystal structure of photosystem II from an SQDG-deficient mutant of Thermosynechococcus elongatus Deposited 2018-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric
Chain z 1–62（62 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 1 CL CHLORIDE ION × 3 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 5 UNL UNKNOWN LIGAND × 25 LMT DODECYL-BETA-D-MALTOSIDE × 10 DMS DIMETHYL SULFOXIDE × 47 CA CALCIUM ION × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 HTG heptyl 1-thio-beta-D-glucopyranoside × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 GOL GLYCEROL × 3 FE2 FE (II) ION × 1 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20MM NACL, 10MM CACL2, 40MM MGSO4, 20MM MES BUFFER, PH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPERATURE 285K
Resolution 2.10 Å R-free 0.197
6DHE RT XFEL structure of the dark-stable state of Photosystem II (0F, S1-rich) at 2.05 Angstrom resolution Deposited 2018-05-20 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 8CT (6'R,11cis,11'cis,13cis,15cis)-4',5'-didehydro-5',6'-dihydro-beta,beta-carotene × 12 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 BCR BETA-CAROTENE × 10 UNL UNKNOWN LIGAND × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.05 Å R-free 0.241
6DHF RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.08 Angstrom resolution Deposited 2018-05-20 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 UNL UNKNOWN LIGAND × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.247
6DHG RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.5 Angstrom resolution Deposited 2018-05-20 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.50 Å R-free 0.246
6DHH RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.2 Angstrom resolution Deposited 2018-05-20 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.20 Å R-free 0.264
6DHO RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.07 Angstrom resolution Deposited 2018-05-20 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.07 Å R-free 0.247
6DHP RT XFEL structure of the three-flash state of Photosystem II (3F, S0-rich) at 2.04 Angstrom resolution Deposited 2018-05-20 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 UNL UNKNOWN LIGAND × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.04 Å R-free 0.248
6W1O RT XFEL structure of the dark-stable state of Photosystem II (0F, S1-rich) at 2.08 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.240
6W1P RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.26 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.26 Å R-free 0.241
6W1Q RT XFEL structure of Photosystem II 50 microseconds after the second illumination at 2.27 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 BCR BETA-CAROTENE × 22 STE STEARIC ACID × 35 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 HEC HEME C × 4 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.27 Å R-free 0.249
6W1R RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.23 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.23 Å R-free 0.235
6W1T RT XFEL structure of Photosystem II 250 microseconds after the second illumination at 2.01 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.01 Å R-free 0.228
6W1U RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.09 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
6W1V RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 STE STEARIC ACID × 38 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 PHO PHEOPHYTIN A × 4 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.241
7NHO Structure of PSII-M Deposited 2021-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain Z 1–62（62 aa）
Not recorded FE FE (III) ION × 1 MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 BCT BICARBONATE ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.66 Å
7NHP Structure of PSII-I (PSII with Psb27, Psb28, and Psb34) Deposited 2021-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain Z 1–62（62 aa）
Not recorded MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 FE FE (III) ION × 1 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.72 Å
7NHQ Structure of PSII-I prime (PSII with Psb28, and Psb34) Deposited 2021-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 17 PDB declaration: heptadecameric
Chain Z 1–62（62 aa）
Not recorded FE FE (III) ION × 1 MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.68 Å
7RF1 RT XFEL structure of Photosystem II averaged across all S-states at 1.89 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 STE STEARIC ACID × 55 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 1.89 Å R-free 0.214
7RF2 RT XFEL structure of dark-stable state of Photosystem II (0F, S1 rich) at 2.08 Angstrom Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 33 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.238
7RF3 RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.26 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.26 Å R-free 0.238
7RF4 RT XFEL structure of Photosystem II 50 microseconds after the second illumination at 2.27 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 BCR BETA-CAROTENE × 22 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.27 Å R-free 0.244
7RF5 RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.23 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.23 Å R-free 0.233
7RF6 RT XFEL structure of Photosystem II 250 microseconds after the second illumination at 2.01 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.01 Å R-free 0.227
7RF7 RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.09 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
7RF8 RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
7YQ2 Crystal structure of photosystem II expressing psbA2 gene only Deposited 2022-08-05 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 39 PDB declaration: 39-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 UNL UNKNOWN LIGAND × 77 LMT DODECYL-BETA-D-MALTOSIDE × 19 GOL GLYCEROL × 10 BCT BICARBONATE ION × 3 DMS DIMETHYL SULFOXIDE × 116 CA CALCIUM ION × 7 HTG heptyl 1-thio-beta-D-glucopyranoside × 31 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 9 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 MG MAGNESIUM ION × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20mM NaCl, 10mM CaCl2, 40mM MgSO4, 20mM Mes BUFFER, pH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPARATURE 285K
Resolution 1.90 Å R-free 0.184
7YQ7 Crystal structure of photosystem II expressing psbA3 gene only Deposited 2022-08-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 39 PDB declaration: 39-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 UNL UNKNOWN LIGAND × 82 GOL GLYCEROL × 11 LMT DODECYL-BETA-D-MALTOSIDE × 18 BCT BICARBONATE ION × 4 DMS DIMETHYL SULFOXIDE × 71 CA CALCIUM ION × 5 HTG heptyl 1-thio-beta-D-glucopyranoside × 18 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 MG MAGNESIUM ION × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20mM NaCl, 10mM CaCl2, 40mM MgSO4, 20mM MES BUFFER pH 6.0, MICRO-BATCH METHOD UNDER OIL, TMPERATURE 285K
Resolution 1.90 Å R-free 0.177
8EZ5 RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2022-10-31 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.234
8F4C RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.224
8F4D RT XFEL structure of Photosystem II 50 microseconds after the third illumination at 2.15 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.15 Å R-free 0.243
8F4E RT XFEL structure of Photosystem II 250 microseconds after the third illumination at 2.09 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.233
8F4F RT XFEL structure of Photosystem II 500 microseconds after the third illumination at 2.03 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.03 Å R-free 0.248
8F4G RT XFEL structure of Photosystem II 730 microseconds after the third illumination at 2.03 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.03 Å R-free 0.223
8F4H RT XFEL structure of Photosystem II 1200 microseconds after the third illumination at 2.10 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.10 Å R-free 0.232
8F4I RT XFEL structure of Photosystem II 2000 microseconds after the third illumination at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.232
8F4J RT XFEL structure of Photosystem II 4000 microseconds after the third illumination at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.226
8F4K RT XFEL structure of the three-flash state of Photosystem II (3F, S0-rich) at 2.16 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.16 Å R-free 0.241
9EVX cryoEM structure of Photosystem II averaged across S2-S3 states at 1.71 Angstrom resolution Deposited 2024-04-02 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 STE STEARIC ACID × 42 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 PHO PHEOPHYTIN A × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；100mM MES pH6.5, 5mM CaCl2, 5% glycerol, 0.03% DDM
cryo-EM vitrification conditions Cryogen ETHANE；The sample-on-grid was illuminated with one and two flashes of light
Resolution 1.71 Å
9G6F Inactive PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LFA EICOSANE × 33 PLM PALMITIC ACID × 24 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 8 LMT DODECYL-BETA-D-MALTOSIDE × 13 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å
9G6G Semi-active PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 3 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 LFA EICOSANE × 30 OEX CA-MN4-O5 CLUSTER × 1 LMT DODECYL-BETA-D-MALTOSIDE × 17 BCT BICARBONATE ION × 2 PLM PALMITIC ACID × 29 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 GOL GLYCEROL × 1 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 3 RRX (3R)-beta,beta-caroten-3-ol × 2 CA CALCIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å
9G6H active PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric
Chain Z 1–62（62 aa）
Chain z 1–62（62 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 PLM PALMITIC ACID × 35 LFA EICOSANE × 24 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 GOL GLYCEROL × 2 LMT DODECYL-BETA-D-MALTOSIDE × 17 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 RRX (3R)-beta,beta-caroten-3-ol × 2 CA CALCIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å

63 other PDB entries and 68 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PSBZ_THEEB
Isoform —
PDB entities 19
Chains and sequence ranges Author chain Z； PDBConstruct 1–62； UniProt 1–62 Author chain z； PDBConstruct 1–62； UniProt 1–62

Photosystem II protein Y

OrganismNot specified

UniProt Q8DKM3

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 40 PDB declaration: 40-meric（40） Consistent with protein copy count Chain R； UniProt 1–41 Chain r； UniProt 1–41 Not recorded Photosystem II protein D1 1 × 2 （P0A444） Photosystem II CP47 reaction center protein × 2 （Q8DIQ1） Photosystem II CP43 reaction center protein × 2 （Q8DIF8） Photosystem II D2 protein × 2 （Q8CM25） Cytochrome b559 subunit alpha × 2 （Q8DIP0） Cytochrome b559 subunit beta × 2 （Q8DIN9） Photosystem II reaction center protein H × 2 （Q8DJ43） Photosystem II reaction center protein I × 2 （Q8DJZ6） Photosystem II reaction center protein J × 2 （P59087） Photosystem II reaction center protein K × 2 （Q9F1K9） Photosystem II reaction center protein L × 2 （Q8DIN8） Photosystem II reaction center protein M × 2 （Q8DHA7） Photosystem II manganese-stabilizing polypeptide × 2 （P0A431） Photosystem II reaction center protein T × 2 （Q8DIQ0） Photosystem II 12 kDa extrinsic protein × 2 （Q9F1L5） Cytochrome c-550 × 2 （P0A386） Photosystem II reaction center protein Ycf12 × 2 （Q8DJI1） Photosystem II reaction center X protein × 2 （Q9F1R6） Photosystem II reaction center protein Z × 2 （Q8DHJ2） OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 UNL UNKNOWN LIGAND × 17 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION X-ray crystallization conditions：BATCH MODE；pH 7.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol. Resolution 3.00 Å R-free 0.303

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5KAF Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
11YO Single-conformation model re-refinement of 2F/S3-rich PSII intermediate structure at 2.09 Angstrom resolution Deposited 2026-03-18 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain R 1–41（41 aa）
Chain r 1–41（41 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 38 OEX CA-MN4-O5 CLUSTER × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 PHO PHEOPHYTIN A × 4 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.243
4IL6 Structure of Sr-substituted photosystem II Deposited 2012-12-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 39 PDB declaration: 39-meric
Chain R 2–35（34 aa） Fragment：UNP residues 2-35
Not recorded OER SR-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 DMS DIMETHYL SULFOXIDE × 35 UNL UNKNOWN LIGAND × 24 LMT DODECYL-BETA-D-MALTOSIDE × 15 GOL GLYCEROL × 12 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HTG heptyl 1-thio-beta-D-glucopyranoside × 17 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions Oil-Microbatch Method；pH 6.1；285 K；Sr-PSII protein was crystallised in 4.5-5.5%PEG1450, 20mM Mes-NaOH, pH 6.1, 20mM NaCl, 3-4mM SrCl2, Oil-Microbatch Method, temperature 285K
Resolution 2.10 Å R-free 0.205
4PJ0 Structure of T.elongatus Photosystem II, rows of dimers crystal packing Deposited 2014-05-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain R 1–41（41 aa）
Chain r 1–41（41 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE FE (III) ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SO4 SULFATE ION × 10 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 29 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；pH 7.5；293 K；Batch crystallisation: 2 mM Chl a equivalent of PSIIcc mixed 1:1 with: 0.1 M TRIS (pH 7.5), 0.1 M (NH4)2SO4, 15 - 18 % PEG 5000 MME, post-crystallisation dehydration
Resolution 2.44 Å R-free 0.256
5KAI NH3-bound RT XFEL structure of Photosystem II 500 ms after the 2nd illumination (2F) at 2.8 A resolution Deposited 2016-06-01 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain R 1–41（41 aa）
Chain r 1–41（41 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 UNL UNKNOWN LIGAND × 23 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 7.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol
Resolution 2.80 Å R-free 0.300
5MX2 Photosystem II depleted of the Mn4CaO5 cluster at 2.55 A resolution Deposited 2017-01-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain R 1–41（41 aa）
Not recorded CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 11 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LFA EICOSANE × 19 FE FE (III) ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 4 BCT BICARBONATE ION × 1 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 7.5；291 K；equal volumes of buffer: 0.1 M Tris-HCl pH 7.5, 0.1 M (NH4)2SO4, 14-18 % PEG 5000 MME and protein: 2 mM chlorophyll equivalent of PSII in C12E8 detergent, post crystallisation treatment
Resolution 2.20 Å R-free 0.260
5MX2 Photosystem II depleted of the Mn4CaO5 cluster at 2.55 A resolution Deposited 2017-01-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain r 1–41（41 aa）
Not recorded CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 11 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LFA EICOSANE × 20 FE FE (III) ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 4 BCT BICARBONATE ION × 1 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 HEC HEME C × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 7.5；291 K；equal volumes of buffer: 0.1 M Tris-HCl pH 7.5, 0.1 M (NH4)2SO4, 14-18 % PEG 5000 MME and protein: 2 mM chlorophyll equivalent of PSII in C12E8 detergent, post crystallisation treatment
Resolution 2.20 Å R-free 0.260
5TIS Room temperature XFEL structure of the native, doubly-illuminated photosystem II complex Deposited 2016-10-03 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain R 1–41（41 aa）
Chain r 1–41（41 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 UNL UNKNOWN LIGAND × 33 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol
Resolution 2.25 Å R-free 0.231
6DHE RT XFEL structure of the dark-stable state of Photosystem II (0F, S1-rich) at 2.05 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain R 2–35（34 aa）
Chain r 2–35（34 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 8CT (6'R,11cis,11'cis,13cis,15cis)-4',5'-didehydro-5',6'-dihydro-beta,beta-carotene × 12 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 BCR BETA-CAROTENE × 10 UNL UNKNOWN LIGAND × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.05 Å R-free 0.241
6DHF RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.08 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain R 2–35（34 aa）
Chain r 2–35（34 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 UNL UNKNOWN LIGAND × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.247
6DHG RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.5 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain R 2–35（34 aa）
Chain r 2–35（34 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.50 Å R-free 0.246
6DHH RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.2 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain R 2–35（34 aa）
Chain r 2–35（34 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.20 Å R-free 0.264
6DHO RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.07 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain R 2–35（34 aa）
Chain r 2–35（34 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.07 Å R-free 0.247
6DHP RT XFEL structure of the three-flash state of Photosystem II (3F, S0-rich) at 2.04 Angstrom resolution Deposited 2018-05-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain R 2–35（34 aa）
Chain r 2–35（34 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 UNL UNKNOWN LIGAND × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.04 Å R-free 0.248
6W1O RT XFEL structure of the dark-stable state of Photosystem II (0F, S1-rich) at 2.08 Angstrom resolution Deposited 2020-03-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain R 2–41（40 aa）
Chain r 2–41（40 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.240
6W1P RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.26 Angstrom resolution Deposited 2020-03-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain R 2–41（40 aa）
Chain r 2–41（40 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.26 Å R-free 0.241
6W1Q RT XFEL structure of Photosystem II 50 microseconds after the second illumination at 2.27 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain R 1–41（41 aa）
Chain r 1–41（41 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 BCR BETA-CAROTENE × 22 STE STEARIC ACID × 35 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 HEC HEME C × 4 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.27 Å R-free 0.249
6W1R RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.23 Angstrom resolution Deposited 2020-03-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain R 2–41（40 aa）
Chain r 2–41（40 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.23 Å R-free 0.235
6W1T RT XFEL structure of Photosystem II 250 microseconds after the second illumination at 2.01 Angstrom resolution Deposited 2020-03-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain R 2–41（40 aa）
Chain r 2–41（40 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.01 Å R-free 0.228
6W1U RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.09 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain R 1–41（41 aa）
Chain r 1–41（41 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
6W1V RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2020-03-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain R 1–41（41 aa）
Chain r 1–41（41 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 STE STEARIC ACID × 38 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 PHO PHEOPHYTIN A × 4 HEC HEME C × 4 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.241
7RF1 RT XFEL structure of Photosystem II averaged across all S-states at 1.89 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain R 1–41（41 aa）
Chain r 1–41（41 aa）
Not recorded FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 STE STEARIC ACID × 55 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 1.89 Å R-free 0.214
7RF2 RT XFEL structure of dark-stable state of Photosystem II (0F, S1 rich) at 2.08 Angstrom Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain R 1–41（41 aa）
Chain r 1–41（41 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 33 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.08 Å R-free 0.238
7RF3 RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.26 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain R 1–41（41 aa）
Chain r 1–41（41 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.26 Å R-free 0.238
7RF4 RT XFEL structure of Photosystem II 50 microseconds after the second illumination at 2.27 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain R 1–41（41 aa）
Chain r 1–41（41 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 BCR BETA-CAROTENE × 22 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
Resolution 2.27 Å R-free 0.244
7RF5 RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.23 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain R 1–41（41 aa）
Chain r 1–41（41 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.23 Å R-free 0.233
7RF6 RT XFEL structure of Photosystem II 250 microseconds after the second illumination at 2.01 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain R 1–41（41 aa）
Chain r 1–41（41 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.01 Å R-free 0.227
7RF7 RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.09 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain R 1–41（41 aa）
Chain r 1–41（41 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
7RF8 RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2021-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain R 1–41（41 aa）
Chain r 1–41（41 aa）
Not recorded FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.239
7YQ2 Crystal structure of photosystem II expressing psbA2 gene only Deposited 2022-08-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 39 PDB declaration: 39-meric
Chain R 1–41（41 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 UNL UNKNOWN LIGAND × 77 LMT DODECYL-BETA-D-MALTOSIDE × 19 GOL GLYCEROL × 10 BCT BICARBONATE ION × 3 DMS DIMETHYL SULFOXIDE × 116 CA CALCIUM ION × 7 HTG heptyl 1-thio-beta-D-glucopyranoside × 31 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 9 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 MG MAGNESIUM ION × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20mM NaCl, 10mM CaCl2, 40mM MgSO4, 20mM Mes BUFFER, pH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPARATURE 285K
Resolution 1.90 Å R-free 0.184
7YQ7 Crystal structure of photosystem II expressing psbA3 gene only Deposited 2022-08-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 39 PDB declaration: 39-meric
Chain R 1–41（41 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 UNL UNKNOWN LIGAND × 82 GOL GLYCEROL × 11 LMT DODECYL-BETA-D-MALTOSIDE × 18 BCT BICARBONATE ION × 4 DMS DIMETHYL SULFOXIDE × 71 CA CALCIUM ION × 5 HTG heptyl 1-thio-beta-D-glucopyranoside × 18 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 MG MAGNESIUM ION × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6；285 K；5% PEG 1450, 20mM NaCl, 10mM CaCl2, 40mM MgSO4, 20mM MES BUFFER pH 6.0, MICRO-BATCH METHOD UNDER OIL, TMPERATURE 285K
Resolution 1.90 Å R-free 0.177
8EZ5 RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2022-10-31 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain R 1–41（41 aa）
Chain r 1–41（41 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.234
8F4C RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain R 1–41（41 aa）
Chain r 1–41（41 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.224
8F4D RT XFEL structure of Photosystem II 50 microseconds after the third illumination at 2.15 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain R 1–41（41 aa）
Chain r 1–41（41 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.15 Å R-free 0.243
8F4E RT XFEL structure of Photosystem II 250 microseconds after the third illumination at 2.09 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain R 1–41（41 aa）
Chain r 1–41（41 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.09 Å R-free 0.233
8F4F RT XFEL structure of Photosystem II 500 microseconds after the third illumination at 2.03 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain R 1–41（41 aa）
Chain r 1–41（41 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.03 Å R-free 0.248
8F4G RT XFEL structure of Photosystem II 730 microseconds after the third illumination at 2.03 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain R 1–41（41 aa）
Chain r 1–41（41 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.03 Å R-free 0.223
8F4H RT XFEL structure of Photosystem II 1200 microseconds after the third illumination at 2.10 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain R 1–41（41 aa）
Chain r 1–41（41 aa）
Not recorded OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.10 Å R-free 0.232
8F4I RT XFEL structure of Photosystem II 2000 microseconds after the third illumination at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain R 1–41（41 aa）
Chain r 1–41（41 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.232
8F4J RT XFEL structure of Photosystem II 4000 microseconds after the third illumination at 2.00 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain R 1–41（41 aa）
Chain r 1–41（41 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.00 Å R-free 0.226
8F4K RT XFEL structure of the three-flash state of Photosystem II (3F, S0-rich) at 2.16 Angstrom resolution Deposited 2022-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric
Chain R 1–41（41 aa）
Chain r 1–41（41 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
Resolution 2.16 Å R-free 0.241
8GN1 Crystal structure of DBBQ-bound photosystem II complex Deposited 2022-08-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 39 PDB declaration: 39-meric
Chain R 1–41（41 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 LMT DODECYL-BETA-D-MALTOSIDE × 20 UNL UNKNOWN LIGAND × 44 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 K2I 2,5-bis(bromanyl)cyclohexa-2,5-diene-1,4-dione × 4 BCT BICARBONATE ION × 2 DMS DIMETHYL SULFOXIDE × 128 CA CALCIUM ION × 5 HTG heptyl 1-thio-beta-D-glucopyranoside × 21 GOL GLYCEROL × 21 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 MG MAGNESIUM ION × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6.1；285 K；5% PEG1450, 20mM NaCl, 10mM CaCl2, 40mM MgSO4, 20mM MES buffer, pH 6.1, MICRO-BATCH METHOD UNDER OIL, temperature 285K
Resolution 2.10 Å R-free 0.191
8GN2 Crystal structure of PPBQ-bound photosystem II complex Deposited 2022-08-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 39 PDB declaration: 39-meric
Chain R 1–41（41 aa）
Not recorded OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 LMT DODECYL-BETA-D-MALTOSIDE × 17 GOL GLYCEROL × 48 UNL UNKNOWN LIGAND × 57 K3C 2-phenylcyclohexa-2,5-diene-1,4-dione × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 BCT BICARBONATE ION × 2 CA CALCIUM ION × 7 HTG heptyl 1-thio-beta-D-glucopyranoside × 21 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DMS DIMETHYL SULFOXIDE × 31 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 MG MAGNESIUM ION × 2 HEC HEME C × 2 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6.1；285 K；5% PEG1450, 20mM NaCl, 10mM CaCl2, 40mM MgSO4, 20mM MES buffer, pH 6.1, MICRO-BATCH METHOD UNDER OIL, temperature 285K
Resolution 1.95 Å R-free 0.181

41 other PDB entries and 42 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PSBY_THEEB
Isoform —
PDB entities 20
Chains and sequence ranges Author chain R； PDBConstruct 1–41； UniProt 1–41 Author chain r； PDBConstruct 1–41； UniProt 1–41

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

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P(r) Distance Distribution P(r) Distribution

暂无 P(r) 图
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2. Structure Basics 2. Structure Basics

Entry ID entry_id5kaf
Deposition date deposition_date2016-06-01
Structure title titleRT XFEL structure of Photosystem II in the dark state at 3.0 A resolution
Keywords keywordsPHOTOSYSTEMS, TRANSMEMBRANE, ROOM TEMPERATURE, ELECTRON TRANSPORT; ELECTRON TRANSPORT
Experimental Method methodX-RAY DIFFRACTION
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

该条目暂无 SAXS 数据。

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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

该条目暂无 P(r) 分析数据。

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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (36)

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7. Fold Classification (SCOP + CATH) 39 domains

SCOP 2.08 (21 domains)

Domain ID domain_idd5kafa_
Class classf — Membrane and cell surface proteins and peptides
Fold Fold foldf.26 — Bacterial photosystem II reaction centre, L and M subunits
Superfamily Superfamily superfamilyf.26.1 — Bacterial photosystem II reaction centre, L and M subunits
Family Family familyf.26.1.1 — Bacterial photosystem II reaction centre, L and M subunits
Domain ID domain_idd5kafb_
Class classf — Membrane and cell surface proteins and peptides
Fold Fold foldf.55 — Photosystem II antenna protein-like
Superfamily Superfamily superfamilyf.55.1 — Photosystem II antenna protein-like
Family Family familyf.55.1.1 — Photosystem II antenna protein-like
Domain ID domain_idd5kafc_
Class classf — Membrane and cell surface proteins and peptides
Fold Fold foldf.55 — Photosystem II antenna protein-like
Superfamily Superfamily superfamilyf.55.1 — Photosystem II antenna protein-like
Family Family familyf.55.1.1 — Photosystem II antenna protein-like
Domain ID domain_idd5kafd_
Class classf — Membrane and cell surface proteins and peptides
Fold Fold foldf.26 — Bacterial photosystem II reaction centre, L and M subunits
Superfamily Superfamily superfamilyf.26.1 — Bacterial photosystem II reaction centre, L and M subunits
Family Family familyf.26.1.1 — Bacterial photosystem II reaction centre, L and M subunits
Domain ID domain_idd5kafe_
Class classf — Membrane and cell surface proteins and peptides
Fold Fold foldf.23 — Single transmembrane helix
Superfamily Superfamily superfamilyf.23.38 — Cytochrome b559 subunits
Family Family familyf.23.38.1 — Cytochrome b559 subunits
Domain ID domain_idd5kaff_
Class classf — Membrane and cell surface proteins and peptides
Fold Fold foldf.23 — Single transmembrane helix
Superfamily Superfamily superfamilyf.23.38 — Cytochrome b559 subunits
Family Family familyf.23.38.1 — Cytochrome b559 subunits
Domain ID domain_idd5kafh_
Class classf — Membrane and cell surface proteins and peptides
Fold Fold foldf.23 — Single transmembrane helix
Superfamily Superfamily superfamilyf.23.33 — Photosystem II 10 kDa phosphoprotein PsbH
Family Family familyf.23.33.1 — PsbH-like
Domain ID domain_idd5kafi1
Class classf — Membrane and cell surface proteins and peptides
Fold Fold foldf.23 — Single transmembrane helix
Superfamily Superfamily superfamilyf.23.37 — Photosystem II reaction center protein I, PsbI
Family Family familyf.23.37.1 — PsbI-like
Domain ID domain_idd5kafi2
Class classl — Artifacts
Fold Fold foldl.1 — Tags
Superfamily Superfamily superfamilyl.1.1 — Tags
Family Family familyl.1.1.1 — Tags
Domain ID domain_idd5kafj_
Class classf — Membrane and cell surface proteins and peptides
Fold Fold foldf.23 — Single transmembrane helix
Superfamily Superfamily superfamilyf.23.32 — Photosystem II reaction center protein J, PsbJ
Family Family familyf.23.32.1 — PsbJ-like
Domain ID domain_idd5kafk_
Class classf — Membrane and cell surface proteins and peptides
Fold Fold foldf.23 — Single transmembrane helix
Superfamily Superfamily superfamilyf.23.36 — Photosystem II reaction center protein K, PsbK
Family Family familyf.23.36.0 — automated matches
Domain ID domain_idd5kafl_
Class classf — Membrane and cell surface proteins and peptides
Fold Fold foldf.23 — Single transmembrane helix
Superfamily Superfamily superfamilyf.23.31 — Photosystem II reaction center protein L, PsbL
Family Family familyf.23.31.1 — PsbL-like
Domain ID domain_idd5kafm1
Class classf — Membrane and cell surface proteins and peptides
Fold Fold foldf.23 — Single transmembrane helix
Superfamily Superfamily superfamilyf.23.35 — Photosystem II reaction center protein M, PsbM
Family Family familyf.23.35.1 — PsbM-like
Domain ID domain_idd5kafm2
Class classl — Artifacts
Fold Fold foldl.1 — Tags
Superfamily Superfamily superfamilyl.1.1 — Tags
Family Family familyl.1.1.1 — Tags
Domain ID domain_idd5kafo_
Class classf — Membrane and cell surface proteins and peptides
Fold Fold foldf.4 — Transmembrane beta-barrels
Superfamily Superfamily superfamilyf.4.1 — OMPA-like
Family Family familyf.4.1.4 — PsbO-like
Domain ID domain_idd5kaft1
Class classf — Membrane and cell surface proteins and peptides
Fold Fold foldf.23 — Single transmembrane helix
Superfamily Superfamily superfamilyf.23.34 — Photosystem II reaction center protein T, PsbT
Family Family familyf.23.34.1 — PsbT-like
Domain ID domain_idd5kaft2
Class classl — Artifacts
Fold Fold foldl.1 — Tags
Superfamily Superfamily superfamilyl.1.1 — Tags
Family Family familyl.1.1.1 — Tags
Domain ID domain_idd5kafu_
Class classa — All alpha proteins
Fold Fold folda.60 — SAM domain-like
Superfamily Superfamily superfamilya.60.12 — PsbU/PolX domain-like
Family Family familya.60.12.2 — PsbU-like
Domain ID domain_idd5kafv_
Class classa — All alpha proteins
Fold Fold folda.3 — Cytochrome c
Superfamily Superfamily superfamilya.3.1 — Cytochrome c
Family Family familya.3.1.0 — automated matches
Domain ID domain_idd5kafx_
Class classf — Membrane and cell surface proteins and peptides
Fold Fold foldf.23 — Single transmembrane helix
Superfamily Superfamily superfamilyf.23.40 — Photosystem II reaction center protein X, PsbX
Family Family familyf.23.40.1 — PsbX-like
Domain ID domain_idd5kafz_
Class classf — Membrane and cell surface proteins and peptides
Fold Fold foldf.17 — Transmembrane helix hairpin
Superfamily Superfamily superfamilyf.17.5 — PsbZ-like
Family Family familyf.17.5.1 — PsbZ-like

CATH v4.4 (18 domains)

Domain ID domain_id5kafB02
Class class3 — Alpha Beta
Architecture architecture10 — Roll
Topology topology680 — Photosystem II CP47 reaction center protein
Homologous superfamily homologous superfamily10 — Photosystem II CP47 reaction center protein
Domain ID domain_id5kafC02
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology10 — Arc Repressor Mutant, subunit A
Homologous superfamily homologous superfamily670 — photosystem ii from thermosynechococcus elongatus
Domain ID domain_id5kafE00
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology5 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Homologous superfamily homologous superfamily860 — Photosystem II cytochrome b559, alpha subunit
Domain ID domain_id5kafH01
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology5 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Homologous superfamily homologous superfamily880 — Photosystem II reaction center protein H
Domain ID domain_id5kafO01
Class class2 — Mainly Beta
Architecture architecture40 — Beta Barrel
Topology topology160 — Porin
Homologous superfamily homologous superfamily30 — Photosystem II, cytochrome c-550 precursor
Domain ID domain_id5kafO02
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology2050 — photosynthetic oxygen evolving center fold
Homologous superfamily homologous superfamily10 — photosynthetic oxygen evolving center domain
Domain ID domain_id5kafU01
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology150 — DNA polymerase; domain 1
Homologous superfamily homologous superfamily320 — Photosystem II 12 kDa extrinsic protein
Domain ID domain_id5kafV00
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology760 — Cytochrome Bc1 Complex; Chain D, domain 2
Homologous superfamily homologous superfamily10 — Cytochrome c-like domain
Domain ID domain_id5kafZ00
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology287 — Helix Hairpins
Homologous superfamily homologous superfamily740 — Photosystem II PsbZ, reaction centre
Domain ID domain_id5kafb02
Class class3 — Alpha Beta
Architecture architecture10 — Roll
Topology topology680 — Photosystem II CP47 reaction center protein
Homologous superfamily homologous superfamily10 — Photosystem II CP47 reaction center protein
Domain ID domain_id5kafc02
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology10 — Arc Repressor Mutant, subunit A
Homologous superfamily homologous superfamily670 — photosystem ii from thermosynechococcus elongatus
Domain ID domain_id5kafe00
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology5 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Homologous superfamily homologous superfamily860 — Photosystem II cytochrome b559, alpha subunit
Domain ID domain_id5kafh01
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology5 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Homologous superfamily homologous superfamily880 — Photosystem II reaction center protein H
Domain ID domain_id5kafo01
Class class2 — Mainly Beta
Architecture architecture40 — Beta Barrel
Topology topology160 — Porin
Homologous superfamily homologous superfamily30 — Photosystem II, cytochrome c-550 precursor
Domain ID domain_id5kafo02
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology2050 — photosynthetic oxygen evolving center fold
Homologous superfamily homologous superfamily10 — photosynthetic oxygen evolving center domain
Domain ID domain_id5kafu01
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology150 — DNA polymerase; domain 1
Homologous superfamily homologous superfamily320 — Photosystem II 12 kDa extrinsic protein
Domain ID domain_id5kafv00
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology760 — Cytochrome Bc1 Complex; Chain D, domain 2
Homologous superfamily homologous superfamily10 — Cytochrome c-like domain
Domain ID domain_id5kafz00
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology287 — Helix Hairpins
Homologous superfamily homologous superfamily740 — Photosystem II PsbZ, reaction centre
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8. Citations (3)

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9. Files and Curves (0)