DNA-directed RNA polymerase subunit alpha
OrganismNot specified
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Protein–DNA Heteromer Protein × 6 DNA 1 PDB declaration: heptameric(7) Consistent with all polymer counts | Chain A; UniProt 1–314 Chain B; UniProt 1–314 | Not recorded | DNA-directed RNA polymerase subunit beta × 1 (Q9KWU7) ;DNA-directed RNA polymerase subunit beta' ; × 1 (Q9KWU6) DNA-directed RNA polymerase subunit omega × 1 (Q9EVV4) RNA polymerase sigma factor SigA × 1 (Q9EZJ8) ;DNA (5'-D(*TP*AP*TP*AP*AP*TP*GP*GP*GP*A)-3') ; × 1 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION X-ray crystallization conditions:VAPOR DIFFUSION, HANGING DROP;pH 6.5;295 K;0.1 M MES, pH 6.5, 50 mM MgCl2, 5-8% (w/v) polyethylene glycol, 10% (v/v) isopropanol | Resolution 2.60 Å R-free 0.262 |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 5TJG | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1HQM CRYSTAL STRUCTURE OF THERMUS AQUATICUS CORE RNA POLYMERASE-INCLUDES COMPLETE STRUCTURE WITH SIDE-CHAINS (EXCEPT FOR DISORDERED REGIONS)-FURTHER REFINED FROM ORIGINAL DEPOSITION-CONTAINS ADDITIONAL SEQUENCE INFORMATION Deposited 2000-12-18 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 5 PDB declaration: pentameric |
Chain A
1–314(314 aa)
Chain B
1–314(314 aa)
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 8;298 K;ammonium sulfate, magnesium chloride, Tris-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.30 Å R-free 0.360 |
| 1I6V THERMUS AQUATICUS CORE RNA POLYMERASE-RIFAMPICIN COMPLEX Deposited 2001-03-05 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 5 PDB declaration: pentameric |
Chain A
1–314(314 aa)
Fragment:ALPHA SUBUNIT
Chain B
1–314(314 aa)
Fragment:ALPHA SUBUNIT
|
Not recorded | RFP RIFAMPICIN × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 8;298 K;Tris-HCl, Ammonium Sulfate, Magensium Chloride, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.30 Å R-free 0.359 |
| 1L9U THERMUS AQUATICUS RNA POLYMERASE HOLOENZYME AT 4 A RESOLUTION Deposited 2002-03-26 | Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 6 PDB declaration: hexameric |
Chain A
1–314(314 aa)
Chain B
1–314(314 aa)
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 8;298 K;HEPES, sodium formate, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 4.00 Å |
| 1L9U THERMUS AQUATICUS RNA POLYMERASE HOLOENZYME AT 4 A RESOLUTION Deposited 2002-03-26 | Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer;Protein × 6 PDB declaration: hexameric |
Chain J
1–314(314 aa)
Chain K
1–314(314 aa)
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 8;298 K;HEPES, sodium formate, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 4.00 Å |
| 1L9Z Thermus aquaticus RNA Polymerase Holoenzyme/Fork-Junction Promoter DNA Complex at 6.5 A Resolution Deposited 2002-03-27 | Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Heteromer;Protein × 6 PDB declaration: octameric |
Chain A
1–314(314 aa)
Chain B
1–314(314 aa)
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 8;277 K;ammonium sulfate, Tris, Magnesium chloride, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 6.50 Å |
| 1YNJ Taq RNA polymerase-Sorangicin complex Deposited 2005-01-24 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 6 PDB declaration: hexameric |
Chain A
1–314(314 aa)
Chain B
1–314(314 aa)
|
Not recorded | SRN SORANGICIN A × 1 ZN ZINC ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 8;295 K;Tris, ammonium formate, magnesium formate, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 3.20 Å R-free 0.346 |
| 1YNN Taq RNA polymerase-rifampicin complex Deposited 2005-01-24 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 6 PDB declaration: hexameric |
Chain A
1–314(314 aa)
Chain B
1–314(314 aa)
|
Not recorded | RFP RIFAMPICIN × 1 ZN ZINC ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 8;295 K;Tris, magnesium sulfate, ammonium sulfate, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 3.30 Å R-free 0.331 |
| 2GHO Recombinant Thermus aquaticus RNA polymerase for Structural Studies Deposited 2006-03-27 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 4 PDB declaration: tetrameric |
Chain A
1–314(314 aa)
Chain B
1–314(314 aa)
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6;295 K;0.05 M Na cacodylate
10 mM MgCl2
1.0 M Li2SO4, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 5.00 Å R-free 0.337 |
| 4XLN Crystal structure of T. aquaticus transcription initiation complex containing bubble promoter and RNA Deposited 2015-01-13 | Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer;Protein × 6 PDB declaration: nonameric |
Chain A
1–314(314 aa)
Chain B
1–314(314 aa)
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 8;293 K;ammonium sulfate, magnesium chloride, Tris-HCl
|
Resolution 4.00 Å R-free 0.315 |
| 4XLN Crystal structure of T. aquaticus transcription initiation complex containing bubble promoter and RNA Deposited 2015-01-13 | Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 2 Other combination Heteromer;Protein × 6 PDB declaration: nonameric |
Chain G
1–314(314 aa)
Chain H
1–314(314 aa)
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 8;293 K;ammonium sulfate, magnesium chloride, Tris-HCl
|
Resolution 4.00 Å R-free 0.315 |
| 4XLP Crystal structure of T.aquaticus transcription initiation complex containing upstream fork promoter Deposited 2015-01-13 | Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Heteromer;Protein × 6 PDB declaration: octameric |
Chain A
1–314(314 aa)
Chain B
1–314(314 aa)
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 8;293 K;ammonium sulfate, magnesium chloride, Tris-HCl
|
Resolution 4.00 Å R-free 0.297 |
| 4XLP Crystal structure of T.aquaticus transcription initiation complex containing upstream fork promoter Deposited 2015-01-13 | Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 2 Protein–DNA Heteromer;Protein × 6 PDB declaration: octameric |
Chain G
1–314(314 aa)
Chain H
1–314(314 aa)
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 8;293 K;ammonium sulfate, magnesium chloride, Tris-HCl
|
Resolution 4.00 Å R-free 0.297 |
| 4XLQ Crystal structure of T.aquaticus transcription initiation complex containing upstream fork (-11 base-paired) promoter Deposited 2015-01-13 | Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Heteromer;Protein × 6 PDB declaration: octameric |
Chain A
1–314(314 aa)
Chain B
1–314(314 aa)
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 8;293 K;ammonium sulfate, magnesium chloride, Tris-HCl
|
Resolution 4.60 Å R-free 0.281 |
| 4XLQ Crystal structure of T.aquaticus transcription initiation complex containing upstream fork (-11 base-paired) promoter Deposited 2015-01-13 | Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 2 Protein–DNA Heteromer;Protein × 6 PDB declaration: octameric |
Chain G
1–314(314 aa)
Chain H
1–314(314 aa)
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 8;293 K;ammonium sulfate, magnesium chloride, Tris-HCl
|
Resolution 4.60 Å R-free 0.281 |
| 4XLR Crystal structure of T.aquaticus transcription initiation complex with CarD containing bubble promoter and RNA Deposited 2015-01-13 | Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer;Protein × 7 PDB declaration: decameric |
Chain A
1–314(314 aa)
Chain B
1–314(314 aa)
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 8;293 K;ammonium sulfate, magnesium chloride, Tris-HCl
|
Resolution 4.30 Å R-free 0.310 |
| 4XLR Crystal structure of T.aquaticus transcription initiation complex with CarD containing bubble promoter and RNA Deposited 2015-01-13 | Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 2 Other combination Heteromer;Protein × 7 PDB declaration: decameric |
Chain G
1–314(314 aa)
Chain H
1–314(314 aa)
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 8;293 K;ammonium sulfate, magnesium chloride, Tris-HCl
|
Resolution 4.30 Å R-free 0.310 |
| 4XLS Crystal structure of T. aquaticus transcription initiation complex with CarD containing upstream fork promoter. Deposited 2015-01-13 | Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Heteromer;Protein × 7 PDB declaration: nonameric |
Chain A
1–314(314 aa)
Chain B
1–314(314 aa)
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 8;293 K;ammonium sulfate, magnesium chloride, Tris-HCl
|
Resolution 4.01 Å R-free 0.272 |
| 4XLS Crystal structure of T. aquaticus transcription initiation complex with CarD containing upstream fork promoter. Deposited 2015-01-13 | Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 2 Protein–DNA Heteromer;Protein × 7 PDB declaration: nonameric |
Chain G
1–314(314 aa)
Chain H
1–314(314 aa)
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 8;293 K;ammonium sulfate, magnesium chloride, Tris-HCl
|
Resolution 4.01 Å R-free 0.272 |
| 4XLS Crystal structure of T. aquaticus transcription initiation complex with CarD containing upstream fork promoter. Deposited 2015-01-13 | Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 3 Protein–DNA Heteromer;Protein × 6 PDB declaration: octameric |
Chain A
1–314(314 aa)
Chain B
1–314(314 aa)
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 8;293 K;ammonium sulfate, magnesium chloride, Tris-HCl
|
Resolution 4.01 Å R-free 0.272 |
| 4XLS Crystal structure of T. aquaticus transcription initiation complex with CarD containing upstream fork promoter. Deposited 2015-01-13 | Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 4 Protein–DNA Heteromer;Protein × 6 PDB declaration: octameric |
Chain G
1–314(314 aa)
Chain H
1–314(314 aa)
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 8;293 K;ammonium sulfate, magnesium chloride, Tris-HCl
|
Resolution 4.01 Å R-free 0.272 |
12 other PDB entries and 20 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | RPOA_THEAQ |
| Isoform | — |
| PDB entities | 1 |
| Chains and sequence ranges | Author chain A; PDBConstruct 1–314; UniProt 1–314 Author chain B; PDBConstruct 1–314; UniProt 1–314 |