SAXSdb — 5y7l

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Calcium-activated potassium channel subunit beta-4

Homo sapiens

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count	Chain A； UniProt 45–166	Fragment：UNP RESIDUES 45-166	No other associated polymer	SOLUTION NMR NMR measurement conditions：pH 6.8；293 K；Ionic strength (raw mmCIF value) 75；Pressure 1 NMR sample composition：0.5 mM [U-13C; U-15N] Hbeta4, 25 mM sodium phosphate, 50 mM sodium chloride, 90% H2O/10% D2O \| 90% H2O/10% D2O NMR sample composition：0.5 mM [U-13C; U-15N] Hbeta4, 25 mM sodium phosphate, 50 mM sodium chloride, 100% D2O \| 100% D2O	Resolution not provided

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 5Y7L	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
6V22 Cryo-EM structure of Ca2+-bound hsSlo1-beta4 channel complex Deposited 2019-11-22	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain E 1–210（210 aa） Chain F 1–210（210 aa） Chain G 1–210（210 aa） Chain H 1–210（210 aa）	Not recorded	MG MAGNESIUM ION × 4 CA CALCIUM ION × 8 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 60 CLR CHOLESTEROL × 16 K POTASSIUM ION × 4 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds before plunging.	Resolution 3.20 Å
6V35 Cryo-EM structure of Ca2+-free hsSlo1-beta4 channel complex Deposited 2019-11-25	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain E 1–210（210 aa） Chain F 1–210（210 aa） Chain G 1–210（210 aa） Chain H 1–210（210 aa）	Not recorded	PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 40 CLR CHOLESTEROL × 8 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds before plunging.	Resolution 3.50 Å
9CZH Ca2+ bound intermediate state of hSlo1 + beta2N-beta4 channel in detergent. Deposited 2024-08-05	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric	Chain E 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ; Chain F 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ; Chain G 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ; Chain H 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;	Not recorded	CA CALCIUM ION × 8 K POTASSIUM ION × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 10	ELECTRON MICROSCOPY cryo-EM buffer pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w). cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
9CZJ Ca2+ free hSlo1 + beta2N-beta4 channel in detergent. Deposited 2024-08-05	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric	Chain E 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ; Chain F 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ; Chain G 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ; Chain H 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;	Not recorded	CLR CHOLESTEROL × 4 K POTASSIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w). cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.54 Å
9CZK Ca2+ free hSlo1 + beta2N-beta4 channel in nanodisc. Deposited 2024-08-05	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric	Chain E 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ; Chain F 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ; Chain G 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ; Chain H 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;	Not recorded	K POTASSIUM ION × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 CLR CHOLESTEROL × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w). cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.50 Å
9CZM Ca2+ bound open-inactivated hSlo1 + beta2N-beta4 channel in nanodisc. Deposited 2024-08-05	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric	Chain E 15–210（196 aa） Chain F 15–210（196 aa） Chain G 15–210（196 aa） Chain H 15–210（196 aa）	Not recorded	POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 32 MG MAGNESIUM ION × 4 CA CALCIUM ION × 8 CLR CHOLESTEROL × 8 K POTASSIUM ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w). cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.57 Å
9CZO Ca2+ bound intermediate state of hSlo1 + beta2N-beta4 channel in nanodisc. Deposited 2024-08-05	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric	Chain E 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ; Chain F 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ; Chain G 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ; Chain H 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;	Not recorded	CA CALCIUM ION × 8 K POTASSIUM ION × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 11	ELECTRON MICROSCOPY cryo-EM buffer pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w). cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.87 Å
9CZQ Ca2+ bound open-inactivated hSlo1 + beta2N-beta4 channel in detergent. Deposited 2024-08-05	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric	Chain E 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ; Chain F 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ; Chain G 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ; Chain H 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;	Not recorded	MG MAGNESIUM ION × 4 CA CALCIUM ION × 8 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 20 CLR CHOLESTEROL × 11 K POTASSIUM ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w). cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.88 Å
9D18 Ca2+ bound open-inactivated hSlo1 + beta2N-beta4 channel in detergent-conformation 2 of inactivating domain Deposited 2024-08-07	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric	Chain E 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ; Chain F 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ; Chain G 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ; Chain H 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;	Not recorded	MG MAGNESIUM ION × 4 CA CALCIUM ION × 8 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 20 CLR CHOLESTEROL × 11 K POTASSIUM ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w). cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.88 Å
9D19 Ca2+ bound open-inactivated hSlo1 + beta2N-beta4 channel in detergent-conformation 3 of inactivating domain Deposited 2024-08-07	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric	Chain E 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ; Chain F 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ; Chain G 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ; Chain H 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;	Not recorded	MG MAGNESIUM ION × 4 CA CALCIUM ION × 8 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 20 CLR CHOLESTEROL × 11 K POTASSIUM ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w). cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.88 Å

10 other PDB entries and 10 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	KCMB4_HUMAN
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–122； UniProt 45–166

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	5y7l
Deposition date deposition_date	2017-08-17
Structure title title	Solution structure of Hbeta4 extracellular loop of BK potassium channel
Keywords keywords	Beta4, BK, METAL BINDING PROTEIN; METAL BINDING PROTEIN
Experimental Method method	SOLUTION NMR

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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier	15.93 Å
Radius of gyration Rg (electron density) rg_electron	15.87 Å
Forward intensity I(0) i0	1300170000.00
Molecular weight molecular_weight	281890.0 kDa
Excluded volume excluded_volume	342180 Å³
Envelope volume envelope_volume	31887 Å³
Hydration-shell volume shell_volume	15750 Å³
Envelope diameter envelope_diameter	61.9 Å
Shell Rg shell_rg	23.04 Å
Envelope Rg envelope_rg	17.72 Å
Shape Rg shape_rg	15.85 Å
Total Rg total_rg	16.01 Å
Total atoms total_atoms	38020
Residues n_residues	2440
Spherical-harmonic order n_harmonics	20
q range q_range	— – 0.5000 Å⁻¹
Data points n_points	101
Shell type shell_type	directional
Solvent electron density solvent_density	0.3340 e/Å³
Shell contrast contrast_shell	0.0300 e/Å³
CRYSOL version crysol_version	4.1.3

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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax	53.4 Å
Rg (real space) rg_real	15.93 Å
Rg uncertainty (real space) rg_real_error	0.33 Å
I(0) (real space) i0_real	1.3000e+09
I(0) uncertainty (real space) i0_real_error	1.3920e+07
Rg (reciprocal space) rg_reciprocal	15.93 Å
I(0) (reciprocal space) i0_reciprocal	1300000000.0000
Solution quality estimate total_estimate	0.8852
Solution quality rating solution_quality	GOOD a GOOD solution
P(r) peaks n_peaks	2
Primary peak position r_peak_primary	18.9 Å
Skewness Skewness skewness	0.318
Kurtosis Kurtosis kurtosis	-0.279
Angular range angular_range	— – 0.5000 Å⁻¹
Current regularization parameter α current_alpha	0.0000
Highest regularization parameter α highest_alpha	286200.0000
Real-space data points n_real_points	80
GNOM version gnom_version	4.1.3
Quality Criteria quality_criteria	AN1: 0.000; Oscil: 0.856; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.968; Smooth: 0.968

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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (1)

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8. Citations (1)

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Description description	Calcium-activated potassium channel subunit beta-4
Molecular weight formula_weight	14114.6 kDa
Source method src_method	man
Copy count entity_count	1

Polymer type type	polypeptide(L)
Chain IDs strand_id	A
Atom count atom_count	38020

来源生物 organism	Homo sapiens
通用名 common_name	Human
NCBI Class编号 ncbi_taxonomy_id	9606
宿主生物 host_organism	Escherichia coli

fit	/app/data/saxs_computation/y7/5y7l/5y7l.fit
pddf	/app/data/pr_computation/y7/5y7l/5y7l.pr.out
profile	/app/data/saxs_computation/y7/5y7l/5y7l.dat

Solution structure of Hbeta4 extracellular loop of BK potassium channel

1. Protein Identity and Related Structures Protein Identity & Related Structures

Calcium-activated potassium channel subunit beta-4

State in the Current Structure

Other States of the Same Protein in the Database

SAXS scattering curve SAXS Profile

P(r) Distance Distribution P(r) Distribution

2. Structure Basics 2. Structure Basics

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

5. Crystallography and Experiment 5. Crystallography & Experiment

6. Entities and Polymers Entities & Polymers (1)

Entity 1 Entity 1 polymer

Polymer Polymer

来源 Source

8. Citations (1)

9. Files and Curves (10)

Files (7)

Curves (3)