6cri

Structure of the cargo bound AP-1:Arf1:tetherin-Nef stable closed trimer

Method: ELECTRON MICROSCOPY

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Bone marrow stromal antigen 2, Protein Nef chimera

Human immunodeficiency virus 1

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Insufficient information	Heteromer	Protein 24	AP-1 complex subunit beta-1 × 3 （Q10567） ADP-ribosylation factor 1 × 6 （P84077） AP-1 complex subunit gamma-1 × 3 （P22892） AP-1 complex subunit mu-1 × 3 （P35585） AP-1 complex subunit sigma-3 × 3 （Q96PC3） GUANOSINE-5'-TRIPHOSPHATE × 6 MAGNESIUM ION × 6	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	BST2_HUMAN
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain N； PDBConstruct 29–48； UniProt 2–21 Author chain T； PDBConstruct 29–48； UniProt 2–21 Author chain Y； PDBConstruct 29–48； UniProt 2–21 Author chain Z； PDBConstruct 29–48； UniProt 2–21 Author chain c； PDBConstruct 29–48； UniProt 2–21 Author chain d； PDBConstruct 29–48； UniProt 2–21

Bone marrow stromal antigen 2, Protein Nef chimera

Human immunodeficiency virus 1

UniProt Q90VU7

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Insufficient information	Heteromer	Protein 24	AP-1 complex subunit beta-1 × 3 （Q10567） ADP-ribosylation factor 1 × 6 （P84077） AP-1 complex subunit gamma-1 × 3 （P22892） AP-1 complex subunit mu-1 × 3 （P35585） AP-1 complex subunit sigma-3 × 3 （Q96PC3） GUANOSINE-5'-TRIPHOSPHATE × 6 MAGNESIUM ION × 6	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	Q90VU7_9HIV1
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain N； PDBConstruct 59–264； UniProt 1–206 Author chain T； PDBConstruct 59–264； UniProt 1–206 Author chain Y； PDBConstruct 59–264； UniProt 1–206 Author chain Z； PDBConstruct 59–264； UniProt 1–206 Author chain c； PDBConstruct 59–264； UniProt 1–206 Author chain d； PDBConstruct 59–264； UniProt 1–206

AP-1 complex subunit beta-1

Homo sapiens

UniProt Q10567

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Insufficient information	Heteromer	Protein 24	Bone marrow stromal antigen 2, Protein Nef chimera × 6 （Q10589,Q90VU7） ADP-ribosylation factor 1 × 6 （P84077） AP-1 complex subunit gamma-1 × 3 （P22892） AP-1 complex subunit mu-1 × 3 （P35585） AP-1 complex subunit sigma-3 × 3 （Q96PC3） GUANOSINE-5'-TRIPHOSPHATE × 6 MAGNESIUM ION × 6	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	AP1B1_HUMAN
Isoform	Q10567-2
PDB entities	2
Chains and sequence ranges	Author chain B； PDBConstruct 1–570； UniProt 14–583 Author chain I； PDBConstruct 1–570； UniProt 14–583 Author chain J； PDBConstruct 1–570； UniProt 14–583

ADP-ribosylation factor 1

Homo sapiens

UniProt P84077

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Insufficient information	Heteromer	Protein 24	Bone marrow stromal antigen 2, Protein Nef chimera × 6 （Q10589,Q90VU7） AP-1 complex subunit beta-1 × 3 （Q10567） AP-1 complex subunit gamma-1 × 3 （P22892） AP-1 complex subunit mu-1 × 3 （P35585） AP-1 complex subunit sigma-3 × 3 （Q96PC3） GUANOSINE-5'-TRIPHOSPHATE × 6 MAGNESIUM ION × 6	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	ARF1_HUMAN
Isoform	—
PDB entities	3
Chains and sequence ranges	Author chain C； PDBConstruct 1–165； UniProt 17–181 Author chain H； PDBConstruct 1–165； UniProt 17–181 Author chain K； PDBConstruct 1–165； UniProt 17–181 Author chain L； PDBConstruct 1–165； UniProt 17–181 Author chain U； PDBConstruct 1–165； UniProt 17–181 Author chain V； PDBConstruct 1–165； UniProt 17–181

AP-1 complex subunit gamma-1

Mus musculus

UniProt P22892

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Insufficient information	Heteromer	Protein 24	Bone marrow stromal antigen 2, Protein Nef chimera × 6 （Q10589,Q90VU7） AP-1 complex subunit beta-1 × 3 （Q10567） ADP-ribosylation factor 1 × 6 （P84077） AP-1 complex subunit mu-1 × 3 （P35585） AP-1 complex subunit sigma-3 × 3 （Q96PC3） GUANOSINE-5'-TRIPHOSPHATE × 6 MAGNESIUM ION × 6	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	AP1G1_MOUSE
Isoform	—
PDB entities	4
Chains and sequence ranges	Author chain G； PDBConstruct 1–585； UniProt 4–588 Author chain Q； PDBConstruct 1–585； UniProt 4–588 Author chain R； PDBConstruct 1–585； UniProt 4–588

AP-1 complex subunit mu-1

Mus musculus

UniProt P35585

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Insufficient information	Heteromer	Protein 24	Bone marrow stromal antigen 2, Protein Nef chimera × 6 （Q10589,Q90VU7） AP-1 complex subunit beta-1 × 3 （Q10567） ADP-ribosylation factor 1 × 6 （P84077） AP-1 complex subunit gamma-1 × 3 （P22892） AP-1 complex subunit sigma-3 × 3 （Q96PC3） GUANOSINE-5'-TRIPHOSPHATE × 6 MAGNESIUM ION × 6	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	AP1M1_MOUSE
Isoform	—
PDB entities	5
Chains and sequence ranges	Author chain M； PDBConstruct 1–422； UniProt 2–423 Author chain W； PDBConstruct 1–422； UniProt 2–423 Author chain X； PDBConstruct 1–422； UniProt 2–423

AP-1 complex subunit sigma-3

Homo sapiens

UniProt Q96PC3

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Insufficient information	Heteromer	Protein 24	Bone marrow stromal antigen 2, Protein Nef chimera × 6 （Q10589,Q90VU7） AP-1 complex subunit beta-1 × 3 （Q10567） ADP-ribosylation factor 1 × 6 （P84077） AP-1 complex subunit gamma-1 × 3 （P22892） AP-1 complex subunit mu-1 × 3 （P35585） GUANOSINE-5'-TRIPHOSPHATE × 6 MAGNESIUM ION × 6	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	AP1S3_HUMAN
Isoform	Q96PC3-2
PDB entities	6
Chains and sequence ranges	Author chain S； PDBConstruct 1–142； UniProt 1–142 Author chain a； PDBConstruct 1–142； UniProt 1–142 Author chain b； PDBConstruct 1–142； UniProt 1–142

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	6cri
Deposition date deposition_date	2018-03-18
Structure title title	Structure of the cargo bound AP-1:Arf1:tetherin-Nef stable closed trimer
Keywords keywords	AP, HIV, Nef, trafficking, VIRAL PROTEIN, PROTEIN TRANSPORT; VIRAL PROTEIN, PROTEIN TRANSPORT
Experimental Method method	ELECTRON MICROSCOPY

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

6cri__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

6cri__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

6cri__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	71.79 Å
Rg (electron density)	71.11 Å
Total Rg	71.32 Å
Atom count	53049
Residues	6582
Excluded volume	951950 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`6cri__assembly_1__model_1`	24-meric （24）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (8)

Entity 1 Entity 1 polymer

Description description	Bone marrow stromal antigen 2, Protein Nef chimera
Molecular weight formula_weight	29964.3 kDa
Source method src_method	man
Copy count entity_count	6

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	T,N,c,Y,d,Z
Atom count atom_count	53049

MSYYHHHHHHDYDIPTTENLYFQGAMGSASTSYDYCRVPMEDGDKRCKGSDEASEGSGMGGKWSKSSVIGWPAVRERMRRAEPAADGVGAVSRDLEKHGAITSSNTAANNAACAWLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIHSQRRQDILDLWIYHTQGYFPDWQNYTPGPGVRYPLTFGWCYKLVPVEPDKVEEANKGENTSLLHPVSLHGMDDPEREVLEWRFDSRLAFHHVARELHPEYFKNC

Source Source

Source organism organism	Human immunodeficiency virus 1
Common name common_name	HIV-1
NCBI taxonomy ID ncbi_taxonomy_id	11676
Host organism host_organism	Escherichia coli

Entity 2 Entity 2 polymer

Description description	AP-1 complex subunit beta-1
Molecular weight formula_weight	64458.7 kDa
Mutation mutation	K359R, E476K
Source method src_method	man
Copy count entity_count	3

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	B,I,J

EIFELKAELNSDKKEKKKEAVKKVIASMTVGKDVSALFPDVVNCMQTDNLELKKLVYLYLMNYAKSQPDMAIMAVNTFVKDCEDPNPLIRALAVRTMGCIRVDKITEYLCEPLRKCLKDEDPYVRKTAAVCVAKLHDINAQLVEDQGFLDTLKDLISDSNPMVVANAVAALSEIAESHPSSNLLDLNPQSINKLLTALNECTEWGQIFILDCLANYMPKDDREAQSICERVTPRLSHANSAVVLSAVKVLMKFMEMLSKDLDYYGTLLKKLAPPLVTLLSAEPELQYVALRNINLIVQKRPEILKHEMKVFFVKYNDPIYVKLEKLDIMIRLASQANIAQVLAELREYATEVDVDFVRKAVRAIGRCAIKVEQSAERCVSTLLDLIQTKVNYVVQEAIVVIKDIFRKYPNKYESVIATLCENLDSLDEPEARAAMIWIVGEYAERIDNADELLESFLEGFHDKSTQVQLQLLTAIVKLFLKKPTETQELVQQVLSLATQDSDNPDLRDRGYIYWRLLSTDPVAAKEVVLAEKPLISEETDLIEPTLLDELICYIGTLASVYHKPPSAFVE

Source Source

Source organism organism	Homo sapiens
Common name common_name	Human
NCBI taxonomy ID ncbi_taxonomy_id	9606
Host organism host_organism	Escherichia coli

Entity 3 Entity 3 polymer

Description description	ADP-ribosylation factor 1
Molecular weight formula_weight	18936.6 kDa
Mutation mutation	Q71L
Source method src_method	man
Copy count entity_count	6

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	C,H,K,U,L,V

EMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGLDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRHRNWYIQATCATSGDGLYEGLDWLSNQLRNQK

Source Source

Source organism organism	Homo sapiens
Common name common_name	Human
NCBI taxonomy ID ncbi_taxonomy_id	9606
Host organism host_organism	Escherichia coli

Entity 4 Entity 4 polymer

Description description	AP-1 complex subunit gamma-1
Molecular weight formula_weight	66401.1 kDa
Source method src_method	man
Copy count entity_count	3

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	G,Q,R

PIRLRELIRTIRTARTQAEEREMIQKECAAIRSSFREEDNTYRCRNVAKLLYMHMLGYPAHFGQLECLKLIASQKFTDKRIGYLGAMLLLDERQDVHLLMTNCIKNDLNHSTQFVQGLALCTLGCMGSSEMCRDLAGEVEKLLKTSNSYLRKKAALCAVHVIRKVPELMEMFLPATKNLLNEKNHGVLHTSVVLLTEMCERSPDMLAHFRKLVPQLVRILKNLIMSGYSPEHDVSGISDPFLQVRILRLLRILGRNDDDSSEAMNDILAQVATNTETSKNVGNAILYETVLTIMDIKSESGLRVLAINILGRFLLNNDKNIRYVALTSLLKTVQTDHNAVQRHRSTIVDCLKDLDVSIKRRAMELSFALVNGNNIRGMMKELLYFLDSCEPEFKADCASGIFLAAEKYAPSKRWHIDTIMRVLTTAGSYVRDDAVPNLIQLITNSVEMHAYTVQRLYKAILGDYSQQPLVQVAAWCIGEYGDLLVSGQCEEEEPIQVTEDEVLDILESVLISNMSTSVTRGYALTAIMKLSTRFTCTVNRIKKVVSIYGSSIDVELQQRAVEYNALFKKYDHMRSALLERMPVME

Source Source

Source organism organism	Mus musculus
Common name common_name	Mouse
NCBI taxonomy ID ncbi_taxonomy_id	10090
Host organism host_organism	Escherichia coli

Entity 5 Entity 5 polymer

Description description	AP-1 complex subunit mu-1
Molecular weight formula_weight	48475.5 kDa
Source method src_method	man
Copy count entity_count	3

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	M,W,X

SASAVYVLDLKGKVLICRNYRGDVDMSEVEHFMPILMEKEEEGMLSPILAHGGVRFMWIKHNNLYLVATSKKNACVSLVFSFLYKVVQVFSEYFKELEEESIRDNFVIIYELLDELMDFGYPQTTDSKILQEYITQEGHKLETGAPRPPATVTNAVSWRSEGIKYRKNEVFLDVIEAVNLLVSANGNVLRSEIVGSIKMRVFLSGMPELRLGLNDKVLFDNTGRGKSKSVELEDVKFHQCVRLSRFENDRTISFIPPDGEFELMSYRLNTHVKPLIWIESVIEKHSHSRIEYMVKAKSQFKRRSTANNVEIHIPVPNDADSPKFKTTVGSVKWVPENSEIVWSVKSFPGGKEYLMRAHFGLPSVEAEDKEGKPPISVKFEIPYFTTSGIQVRYLKIIEKSGYQALPWVRYITQNGDYQLRTQ

Source Source

Source organism organism	Mus musculus
Common name common_name	Mouse
NCBI taxonomy ID ncbi_taxonomy_id	10090
Host organism host_organism	Escherichia coli

Entity 6 Entity 6 polymer

Description description	AP-1 complex subunit sigma-3
Molecular weight formula_weight	16998.8 kDa
Mutation mutation	C148S
Source method src_method	man
Copy count entity_count	3

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	S,a,b

MIHFILLFSRQGKLRLQKWYITLPDKERKKITREIVQIILSRGHRTSSFVDWKELKLVYKRYASLYFCCAIENQDNELLTLEIVHRYVELLDKYFGNVCELDIIFNFEKAYFILDEFIIGGEIQETSKKIAVKAIEDSDMLQ

Source Source

Source organism organism	Homo sapiens
Common name common_name	Human
NCBI taxonomy ID ncbi_taxonomy_id	9606
Host organism host_organism	Escherichia coli

Entity 7 Entity 7 non-polymer

Description description	GUANOSINE-5'-TRIPHOSPHATE
Molecular weight formula_weight	523.2 kDa
Source method src_method	syn
Copy count entity_count	6

Entity 8 Entity 8 non-polymer

Description description	MAGNESIUM ION
Molecular weight formula_weight	24.3 kDa
Source method src_method	syn
Copy count entity_count	6

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Unit-cell length a length_a	1.000 Å
Unit-cell length b length_b	1.000 Å
Unit-cell length c length_c	1.000 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	1

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	—

Resolution resolution	6.80 Å
Resolution测定Method resolution_method	—
颗粒数 num_particles	11108

Structure of the cargo bound AP-1:Arf1:tetherin-Nef stable closed trimer

1. Protein Identity and Related Structures Protein Identity & Related Structures

Bone marrow stromal antigen 2, Protein Nef chimera

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Bone marrow stromal antigen 2, Protein Nef chimera

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

AP-1 complex subunit beta-1

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

ADP-ribosylation factor 1

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

AP-1 complex subunit gamma-1

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

AP-1 complex subunit mu-1

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

AP-1 complex subunit sigma-3

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

6cri__assembly_1__model_1

6cri__assembly_1__model_1 | I(q)

6cri__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

冷冻电镜 EM 3D

5. Entities and Polymers Entities & Polymers (8)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 polymer

Polymer Polymer

Source Source

Entity 3 Entity 3 polymer

Polymer Polymer

Source Source

Entity 4 Entity 4 polymer

Polymer Polymer

Source Source