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6h03

OPEN CONFORMATION OF THE MEMBRANE ATTACK COMPLEX

Method: ELECTRON MICROSCOPY Dmax: 247.5 Å Quality: EXCELLENT
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Complement C5,Complement C5

OrganismNot specified

UniProt P01031

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Other combination Heteromer Protein × 24 其他Polymer 21 PDB declaration: 24-meric（24） Consistent with protein copy count Chain A； UniProt 19–673 Chain A； UniProt 752–1676 Not recorded Complement component C8 beta chain × 1 （P07358） Complement component C7 × 1 （P10643） Complement component C8 gamma chain × 1 （P07360） Complement component C8 alpha chain × 1 （P07357） Complement component C6 × 1 （P13671） Complement component C9 × 18 （P02748） beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 1 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 20 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 37 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 5.60 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 6H03 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1CFA SOLUTION STRUCTURE OF A SEMI-SYNTHETIC C5A RECEPTOR ANTAGONIST AT PH 5.2, 303K, NMR, 20 STRUCTURES Deposited 1996-09-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 679–747（69 aa） Fragment：RESIDUES 1 - 71
Mutation：Q71C No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 5.2；303 K
Resolution not provided
1KJS NMR SOLUTION STRUCTURE OF C5A AT PH 5.2, 303K, 20 STRUCTURES Deposited 1997-01-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 679–751（73 aa）
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 5.2；303 K
Resolution not provided
1XWE NMR Structure of C345C (NTR) domain of C5 of complement Deposited 2004-10-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1530–1676（147 aa） Fragment：C345C domain
Mutation：F1613A No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6；303 K；Ionic strength (raw mmCIF value) 20mM Na phospahte; 100mM NaCl；Pressure ambient
NMR sample composition 0.5-1.0mM C5-C345C; U-15N,13C; 20mM Na phosphate buffer; 100mM NaCl; 5uM EDTA; pH 6 | 95% H2O/5% D2O
Resolution not provided
3CU7 Human Complement Component 5 Deposited 2008-04-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–1676（1676 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 CD CADMIUM ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；277 K；100mM NaCl, 1.5-3mM MES pH 5.5, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 3.10 Å R-free 0.281
3CU7 Human Complement Component 5 Deposited 2008-04-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Other combination Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1–1676（1676 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 CD CADMIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；277 K；100mM NaCl, 1.5-3mM MES pH 5.5, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 3.10 Å R-free 0.281
3CU7 Human Complement Component 5 Deposited 2008-04-16 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–1676（1676 aa）
Chain B 1–1676（1676 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 CD CADMIUM ION × 9 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；277 K；100mM NaCl, 1.5-3mM MES pH 5.5, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 3.10 Å R-free 0.281
3HQA Crystal structure of human desarg-C5A Deposited 2009-06-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 679–750（72 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.8；298 K；2.4 M sodium chloride, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.59 Å R-free 0.253
3HQA Crystal structure of human desarg-C5A Deposited 2009-06-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 679–750（72 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.8；298 K；2.4 M sodium chloride, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.59 Å R-free 0.253
3HQA Crystal structure of human desarg-C5A Deposited 2009-06-05 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 679–750（72 aa）
Chain B 679–750（72 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.8；298 K；2.4 M sodium chloride, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.59 Å R-free 0.253
3HQB Crystal structure of human desarg-C5A Deposited 2009-06-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 679–750（72 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.8；298 K；2.4 M sodium chloride, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 3.30 Å R-free 0.307
3HQB Crystal structure of human desarg-C5A Deposited 2009-06-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 679–750（72 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.8；298 K；2.4 M sodium chloride, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 3.30 Å R-free 0.307
3HQB Crystal structure of human desarg-C5A Deposited 2009-06-05 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 679–750（72 aa）
Chain B 679–750（72 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.8；298 K；2.4 M sodium chloride, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 3.30 Å R-free 0.307
3KLS Structure of complement C5 in complex with SSL7 Deposited 2009-11-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–1676（1676 aa）
Not recorded CD CADMIUM ION × 5 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.2；277 K；Reservoir contains 50mM MgAc2, 50 mM MES pH 6.2, mixed 1:1 with protein, VAPOR DIFFUSION, temperature 277K
Resolution 3.60 Å R-free 0.263
3KLS Structure of complement C5 in complex with SSL7 Deposited 2009-11-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Other combination Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 1–1676（1676 aa）
Not recorded CD CADMIUM ION × 4 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.2；277 K；Reservoir contains 50mM MgAc2, 50 mM MES pH 6.2, mixed 1:1 with protein, VAPOR DIFFUSION, temperature 277K
Resolution 3.60 Å R-free 0.263
3KM9 Structure of complement C5 in complex with the C-terminal beta-grasp domain of SSL7 Deposited 2009-11-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–1676（1676 aa）
Not recorded CD CADMIUM ION × 3 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.2；277 K；Reservoir contains 50mM MgAc2, 50mM MES pH 6.2. Mixed 1:1 with concentrated protein, VAPOR DIFFUSION, temperature 277K
Resolution 4.20 Å R-free 0.297
3KM9 Structure of complement C5 in complex with the C-terminal beta-grasp domain of SSL7 Deposited 2009-11-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Other combination Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 1–1676（1676 aa）
Not recorded CD CADMIUM ION × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.2；277 K；Reservoir contains 50mM MgAc2, 50mM MES pH 6.2. Mixed 1:1 with concentrated protein, VAPOR DIFFUSION, temperature 277K
Resolution 4.20 Å R-free 0.297
3PRX Structure of Complement C5 in Complex with CVF and SSL7 Deposited 2010-11-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–1676（1676 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.4；292 K；3 M sodium malonate, pH 7.4, VAPOR DIFFUSION, temperature 292K
Resolution 4.30 Å R-free 0.261
3PRX Structure of Complement C5 in Complex with CVF and SSL7 Deposited 2010-11-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Other combination Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 1–1676（1676 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.4；292 K；3 M sodium malonate, pH 7.4, VAPOR DIFFUSION, temperature 292K
Resolution 4.30 Å R-free 0.261
3PVM Structure of Complement C5 in Complex with CVF Deposited 2010-12-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–1676（1676 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 4.30 Å R-free 0.262
3PVM Structure of Complement C5 in Complex with CVF Deposited 2010-12-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 1–1676（1676 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 4.30 Å R-free 0.262
4A5W Crystal structure of C5b6 Deposited 2011-10-28 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 19–673（655 aa）
Chain A 752–1676（925 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 CA CALCIUM ION × 1 FUC alpha-L-fucopyranose × 1 MAN alpha-D-mannopyranose × 3 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.8；167 MM HEPES-NAOH PH7.8
Resolution 3.50 Å R-free 0.270
4E0S Crystal Structure of C5b-6 Deposited 2012-03-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–1676（1676 aa）
Not recorded NA SODIUM ION × 1 CA CALCIUM ION × 1 MAN alpha-D-mannopyranose × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.6；298 K；0.08M lithium chloride, 0.01M imidazole-HCl, pH 7.6, SMALL TUBES, temperature 298K
Resolution 4.21 Å R-free 0.278
4P39 Crystal structure of the human C5aR antagonist C5a-A8 Deposited 2014-03-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 678–747（70 aa） Fragment：UNP residues 678-743
Mutation：C704R No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.6；277 K；20% 2-propanol, 20% PEG 4000, 0.1 M Na citrate pH 5.6
Resolution 2.40 Å R-free 0.238
4P39 Crystal structure of the human C5aR antagonist C5a-A8 Deposited 2014-03-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 678–747（70 aa） Fragment：UNP residues 678-743
Mutation：C704R No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.6；277 K；20% 2-propanol, 20% PEG 4000, 0.1 M Na citrate pH 5.6
Resolution 2.40 Å R-free 0.238
4P39 Crystal structure of the human C5aR antagonist C5a-A8 Deposited 2014-03-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 678–747（70 aa） Fragment：UNP residues 678-743
Mutation：C704R No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.6；277 K；20% 2-propanol, 20% PEG 4000, 0.1 M Na citrate pH 5.6
Resolution 2.40 Å R-free 0.238
4P39 Crystal structure of the human C5aR antagonist C5a-A8 Deposited 2014-03-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain D 678–747（70 aa） Fragment：UNP residues 678-743
Mutation：C704R No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.6；277 K；20% 2-propanol, 20% PEG 4000, 0.1 M Na citrate pH 5.6
Resolution 2.40 Å R-free 0.238
4P39 Crystal structure of the human C5aR antagonist C5a-A8 Deposited 2014-03-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 5 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 678–747（70 aa） Fragment：UNP residues 678-743
Chain B 678–747（70 aa） Fragment：UNP residues 678-743
Chain C 678–747（70 aa） Fragment：UNP residues 678-743
Chain D 678–747（70 aa） Fragment：UNP residues 678-743
Mutation：C704R Mutation：C704R Mutation：C704R Mutation：C704R No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.6；277 K；20% 2-propanol, 20% PEG 4000, 0.1 M Na citrate pH 5.6
Resolution 2.40 Å R-free 0.238
4P39 Crystal structure of the human C5aR antagonist C5a-A8 Deposited 2014-03-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 6 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 678–747（70 aa） Fragment：UNP residues 678-743
Chain D 678–747（70 aa） Fragment：UNP residues 678-743
Mutation：C704R Mutation：C704R No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.6；277 K；20% 2-propanol, 20% PEG 4000, 0.1 M Na citrate pH 5.6
Resolution 2.40 Å R-free 0.238
4P39 Crystal structure of the human C5aR antagonist C5a-A8 Deposited 2014-03-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 7 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain B 678–747（70 aa） Fragment：UNP residues 678-743
Chain C 678–747（70 aa） Fragment：UNP residues 678-743
Mutation：C704R Mutation：C704R No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.6；277 K；20% 2-propanol, 20% PEG 4000, 0.1 M Na citrate pH 5.6
Resolution 2.40 Å R-free 0.238
4UU9 Crystal structure of the human c5a in complex with MEDI7814 a neutralising antibody Deposited 2014-07-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 678–751（74 aa） Fragment：C5A
Not recorded SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 20% PEG10K, 8% ETHYLENE GLYCOL, 100MM HEPES PH 7.5
Resolution 2.12 Å R-free 0.202
4UU9 Crystal structure of the human c5a in complex with MEDI7814 a neutralising antibody Deposited 2014-07-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain D 678–751（74 aa） Fragment：C5A
Not recorded SO4 SULFATE ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions 20% PEG10K, 8% ETHYLENE GLYCOL, 100MM HEPES PH 7.5
Resolution 2.12 Å R-free 0.202
5B4P Complex structure of human C5a and its binding repebody Deposited 2016-04-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 678–751（74 aa）
Not recorded 1PE PENTAETHYLENE GLYCOL × 2 SO4 SULFATE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；Sodium acetate, PEG 400, ammonium sulfate
Resolution 2.40 Å R-free 0.310
5B4P Complex structure of human C5a and its binding repebody Deposited 2016-04-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain D 678–751（74 aa）
Not recorded SO4 SULFATE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；Sodium acetate, PEG 400, ammonium sulfate
Resolution 2.40 Å R-free 0.310
5B71 Crystal structure of complement C5 in complex with SKY59 Deposited 2016-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain E 20–124（105 aa） Fragment：UNP RESIDUES 20-124
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；277 K；0.2M magnesium formate dehydrate, 15.0% w/v polyethylene glycol 3350, 20% v/v glycerol as cryoprotectant
Resolution 2.11 Å R-free 0.285
5B71 Crystal structure of complement C5 in complex with SKY59 Deposited 2016-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain F 20–124（105 aa） Fragment：UNP RESIDUES 20-124
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；277 K；0.2M magnesium formate dehydrate, 15.0% w/v polyethylene glycol 3350, 20% v/v glycerol as cryoprotectant
Resolution 2.11 Å R-free 0.285
5HCC Ternary complex of human Complement C5 with Ornithodoros moubata OmCI and Dermacentor andersoni RaCI3. Deposited 2016-01-04 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 679–1676（998 aa） Fragment：UNP Residues 679-1676
Chain B 19–674（656 aa） Fragment：UNP Residues 19-674
Not recorded EDO 1,2-ETHANEDIOL × 7 CYS CYSTEINE × 1 DIO 1,4-DIETHYLENE DIOXIDE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 9；294 K；10% PEG 20K, 2% v/v 1,4 Dioxane, 0.1 M Bicine pH 9.0
Resolution 2.59 Å R-free 0.267
5HCD Ternary complex of human Complement C5 with Ornithodoros moubata OmCI and Rhipicephalus microplus RaCI2 Deposited 2016-01-04 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 679–1676（998 aa） Fragment：UNP Residues 679-1676
Chain B 19–674（656 aa） Fragment：UNP Residues 19-674
Not recorded CYS CYSTEINE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 9；294 K；10% (w/v) PEG 20K, 2%(v/v)1,4 Dioxane, 0.1M Bicine pH 9.0
Resolution 2.98 Å R-free 0.282
5HCE Ternary complex of human Complement C5 with Ornithodoros moubata OmCI and Rhipicephalus appendiculatus RaCI1 Deposited 2016-01-04 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 679–1676（998 aa） Fragment：UNP Residues 679-1676
Chain B 19–674（656 aa） Fragment：UNP Residues 19-674
Not recorded CYS CYSTEINE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 9；294 K；10% PEG 6K, 0.1 M Bicine pH 9.0
Resolution 3.12 Å R-free 0.281
5I5K Structure of complement C5 in complex with eculizumab Deposited 2016-02-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–1676（1676 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；277.15 K；0.1 M Imidazole pH 6.2 4% v/v Tacsimate pH 7 8% PEG 3350
Resolution 4.20 Å R-free 0.244
5I5K Structure of complement C5 in complex with eculizumab Deposited 2016-02-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Other combination Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–1676（1676 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；277.15 K；0.1 M Imidazole pH 6.2 4% v/v Tacsimate pH 7 8% PEG 3350
Resolution 4.20 Å R-free 0.244
6H04 Closed conformation of the Membrane Attack Complex Deposited 2018-07-06 Different oligomeric state Different ligand/ion Assembly 1 Other combination Heteromer；Protein × 24 PDB declaration: 24-meric
Chain A 19–673（655 aa）
Chain A 752–1676（925 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 38 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.60 Å
6RPT Structure of tick complement inhibitor CirpT1 complexed with macroglobubulin domain 4 of human complement C5 Deposited 2019-05-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain E 348–460（113 aa）
Not recorded GOL GLYCEROL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；284.15 K；0.02 M Na2PO4/K2PO4, 20 % w/v PEG3350
Resolution 2.70 Å R-free 0.272
6RPT Structure of tick complement inhibitor CirpT1 complexed with macroglobubulin domain 4 of human complement C5 Deposited 2019-05-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 348–460（113 aa）
Not recorded GOL GLYCEROL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；284.15 K；0.02 M Na2PO4/K2PO4, 20 % w/v PEG3350
Resolution 2.70 Å R-free 0.272
6RPT Structure of tick complement inhibitor CirpT1 complexed with macroglobubulin domain 4 of human complement C5 Deposited 2019-05-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 348–460（113 aa）
Not recorded GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；284.15 K；0.02 M Na2PO4/K2PO4, 20 % w/v PEG3350
Resolution 2.70 Å R-free 0.272
6RQJ Structure of human complement C5 complexed with tick inhibitors OmCI, RaCI1 and CirpT1 Deposited 2019-05-15 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric
Chain A 678–1676（999 aa）
Chain B 19–673（655 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
7AD6 Crystal structure of human complement C5 in complex with the K92 bovine knob domain peptide. Deposited 2020-09-14 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–1676（1676 aa）
Chain B 1–1676（1676 aa）
Not recorded EDO 1,2-ETHANEDIOL × 3 CYS CYSTEINE × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；296 K；0.1 M bicine/Trizma (pH 8.5), 10 % (w/v) PEG 8000, 20 % (v/v) ethylene glycol, 30 mM sodium fluoride, 30 mM sodium bromide and 30 mM sodium iodide.
Resolution 2.75 Å R-free 0.254
7AD7 Crystal structure of human complement C5 in complex with the K8 bovine knob domain peptide. Deposited 2020-09-14 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–1676（1676 aa）
Chain B 1–1676（1676 aa）
Not recorded EOH ETHANOL × 4 MHA (CARBAMOYLMETHYL-CARBOXYMETHYL-AMINO)-ACETIC ACID × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 CYS CYSTEINE × 1 TAM TRIS(HYDROXYETHYL)AMINOMETHANE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；296 K；0.1M ADA, 14% ethanol.
Resolution 2.30 Å R-free 0.234
7NYC cryoEM structure of 3C9-sMAC Deposited 2021-03-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain A 19–1676（1658 aa）
Not recorded MAN alpha-D-mannopyranose × 7 CA CALCIUM ION × 3 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 FUC alpha-L-fucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.54 Å
7NYD cryoEM structure of 2C9-sMAC Deposited 2021-03-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 8 PDB declaration: octameric
Chain A 19–1676（1658 aa）
Not recorded MAN alpha-D-mannopyranose × 6 CA CALCIUM ION × 4 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 FUC alpha-L-fucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.27 Å
7OP0 Crystal structure of complement C5 in complex with chemically synthesized K92 knob domain. Deposited 2021-05-28 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 678–1676（999 aa）
Chain B 19–675（657 aa） Fragment：beta chain, UNP residues 1-675
Not recorded EDO 1,2-ETHANEDIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；0.1 M bicine/Trizma (pH 8.5), 10 % (w/v) PEG 8000, 20 % (v/v) ethylene glycol, 30 mM sodium fluoride, 30 mM sodium bromide, 30 mM sodium iodide.
Resolution 2.57 Å R-free 0.264
7Y64 Cryo-EM structure of C5a-bound C5aR1 in complex with Gi protein Deposited 2022-06-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain E 678–751（74 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8AYH Structure of Complement C5 in Complex with small molecule inhibitor and CVF Deposited 2022-09-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 678–1676（999 aa）
Chain C 19–675（657 aa） Fragment：beta chain, UNP residues 1-675
Not recorded H1H 5-methoxy-2-[[(1~{S})-1-(2-methoxyphenyl)ethyl]carbamoylamino]-4-(4-methylpentoxy)benzoic acid × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；50mM HEPES pH 7.5 100mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.35 Å
8B0F CryoEM structure of C5b8-CD59 Deposited 2022-09-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain A 1–1676（1676 aa）
Not recorded CA CALCIUM ION × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8B0G 2C9, C5b9-CD59 structure Deposited 2022-09-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 10 PDB declaration: decameric
Chain A 1–1676（1676 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20 mM HEPES pH 7.4, 120 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8B0H 2C9, C5b9-CD59 cryoEM structure Deposited 2022-09-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric
Chain A 1–1676（1676 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20 mM HEPES pH 7.4, 120 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8CML Cryo-EM structure of complement C5 in complex with nanobodies UNbC5-1 and UNbC5-2 Deposited 2023-02-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 4 PDB declaration: tetrameric
Chain B 678–1676（999 aa）
Chain E 19–673（655 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；1x PBS
cryo-EM vitrification conditions Cryogen ETHANE；Blot 4 seconds at blot force 1
Resolution 3.60 Å
8COE complement C5 in complex with the LCP0195 nanobody Deposited 2023-02-28 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 678–1676（999 aa）
Chain C 19–673（655 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；292 K；0.025 M succinic acid pH 7.0 and 3.75% w/v polyethylene glycol 3350.
Resolution 4.20 Å R-free 0.269
8HK5 C5aR1-Gi-C5a protein complex Deposited 2022-11-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain B 678–751（74 aa）
Not recorded PLM PALMITIC ACID × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.2
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8HQC Structure of a GPCR-G protein in complex with a natural peptide agonist Deposited 2022-12-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain D 678–751（74 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.89 Å
8IA2 Structure of C5a bound human C5aR1 in complex with Go (Composite map) Deposited 2023-02-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain D 678–751（74 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.21 Å
8JZZ Structure of human C5a-desArg bound human C5aR1 in complex with Go Deposited 2023-07-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain D 678–751（74 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.31 Å
9KX6 Structure of human C5a-desArg bound mouse C5aR1 in complex with Go Deposited 2024-12-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain D 678–750（73 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.11 Å
9Y6C X-ray structure analysis of human Complement Component 5 TE domain in complex with the peptide Ra30303 Deposited 2025-09-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 982–1305（324 aa）
Not recorded GOL GLYCEROL × 1 8VH 1,3-dimethylbenzene × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；277 K；Proteros
Resolution 2.00 Å R-free 0.253

41 other PDB entries and 63 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name CO5_HUMAN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–655； UniProt 19–673 Author chain A； PDBConstruct 656–1580； UniProt 752–1676

Complement component C8 beta chain

OrganismNot specified

UniProt P07358

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Other combination Heteromer Protein × 24 其他Polymer 21 PDB declaration: 24-meric（24） Consistent with protein copy count Chain C； UniProt 55–591 Not recorded Complement C5,Complement C5 × 1 （P01031） Complement component C7 × 1 （P10643） Complement component C8 gamma chain × 1 （P07360） Complement component C8 alpha chain × 1 （P07357） Complement component C6 × 1 （P13671） Complement component C9 × 18 （P02748） beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 1 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 20 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 37 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 5.60 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 6H03 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3OJY Crystal Structure of Human Complement Component C8 Deposited 2010-08-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 55–591（537 aa） Fragment：unp residues 55-591
Non-standard monomer：Yes (specific site not provided by mmCIF) CA CALCIUM ION × 2 BMA beta-D-mannopyranose × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.4；281 K；14-16% PEG 4K, 100mM TAPS pH 9.0, 400mM NaCl, 10mM SrCl2, VAPOR DIFFUSION, HANGING DROP, temperature 281K
Resolution 2.51 Å R-free 0.337
6H04 Closed conformation of the Membrane Attack Complex Deposited 2018-07-06 Different oligomeric state Different ligand/ion Assembly 1 Other combination Heteromer；Protein × 24 PDB declaration: 24-meric
Chain C 55–591（537 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 38 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.60 Å
7NYC cryoEM structure of 3C9-sMAC Deposited 2021-03-22 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain D 55–591（537 aa）
Not recorded MAN alpha-D-mannopyranose × 7 CA CALCIUM ION × 3 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 FUC alpha-L-fucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.54 Å
7NYD cryoEM structure of 2C9-sMAC Deposited 2021-03-22 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 8 PDB declaration: octameric
Chain D 55–591（537 aa）
Not recorded MAN alpha-D-mannopyranose × 6 CA CALCIUM ION × 4 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 FUC alpha-L-fucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.27 Å
8B0F CryoEM structure of C5b8-CD59 Deposited 2022-09-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain D 1–591（591 aa）
Not recorded CA CALCIUM ION × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8B0G 2C9, C5b9-CD59 structure Deposited 2022-09-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 10 PDB declaration: decameric
Chain D 1–591（591 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20 mM HEPES pH 7.4, 120 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8B0H 2C9, C5b9-CD59 cryoEM structure Deposited 2022-09-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric
Chain D 1–591（591 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20 mM HEPES pH 7.4, 120 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å

7 other PDB entries and 7 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name CO8B_HUMAN
Isoform —
PDB entities 2
Chains and sequence ranges Author chain C； PDBConstruct 1–537； UniProt 55–591

Complement component C7

OrganismNot specified

UniProt P10643

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Other combination Heteromer Protein × 24 其他Polymer 21 PDB declaration: 24-meric（24） Consistent with protein copy count Chain D； UniProt 23–843 Not recorded Complement C5,Complement C5 × 1 （P01031） Complement component C8 beta chain × 1 （P07358） Complement component C8 gamma chain × 1 （P07360） Complement component C8 alpha chain × 1 （P07357） Complement component C6 × 1 （P13671） Complement component C9 × 18 （P02748） beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 1 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 20 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 37 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 5.60 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 6H03 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
2WCY NMR solution structure of factor I-like modules of complement C7. Deposited 2009-03-17 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 693–843（151 aa） Fragment：FACTOR I-LIKE MODULES (FIMS), RESIDUES 693-843
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.5；298 K；Ionic strength (raw mmCIF value) 20MM K PHOSPHATE
NMR sample composition 90% H2O, 10% D2O
Resolution not provided
6H04 Closed conformation of the Membrane Attack Complex Deposited 2018-07-06 Different oligomeric state Different ligand/ion Assembly 1 Other combination Heteromer；Protein × 24 PDB declaration: 24-meric
Chain D 23–843（821 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 38 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.60 Å
7NYC cryoEM structure of 3C9-sMAC Deposited 2021-03-22 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain C 23–843（821 aa）
Not recorded MAN alpha-D-mannopyranose × 7 CA CALCIUM ION × 3 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 FUC alpha-L-fucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.54 Å
7NYD cryoEM structure of 2C9-sMAC Deposited 2021-03-22 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 8 PDB declaration: octameric
Chain C 23–843（821 aa）
Not recorded MAN alpha-D-mannopyranose × 6 CA CALCIUM ION × 4 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 FUC alpha-L-fucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.27 Å
8B0F CryoEM structure of C5b8-CD59 Deposited 2022-09-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain C 1–843（843 aa）
Not recorded CA CALCIUM ION × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8B0G 2C9, C5b9-CD59 structure Deposited 2022-09-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 10 PDB declaration: decameric
Chain C 1–843（843 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20 mM HEPES pH 7.4, 120 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8B0H 2C9, C5b9-CD59 cryoEM structure Deposited 2022-09-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric
Chain C 1–843（843 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20 mM HEPES pH 7.4, 120 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å

7 other PDB entries and 7 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name CO7_HUMAN
Isoform —
PDB entities 3
Chains and sequence ranges Author chain D； PDBConstruct 1–821； UniProt 23–843

Complement component C8 gamma chain

OrganismNot specified

UniProt P07360

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Other combination Heteromer Protein × 24 其他Polymer 21 PDB declaration: 24-meric（24） Consistent with protein copy count Chain E； UniProt 21–202 Not recorded Complement C5,Complement C5 × 1 （P01031） Complement component C8 beta chain × 1 （P07358） Complement component C7 × 1 （P10643） Complement component C8 alpha chain × 1 （P07357） Complement component C6 × 1 （P13671） Complement component C9 × 18 （P02748） beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 1 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 20 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 37 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 5.60 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 6H03 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1IW2 X-ray structure of Human Complement Protein C8gamma at pH=7.O Deposited 2002-04-11 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 21–202（182 aa）
Mutation：C40G No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；277 K；PEG 4000, sodium citrate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.90 Å R-free 0.250
1LF7 Crystal Structure of Human Complement Protein C8gamma at 1.2 A Resolution Deposited 2002-04-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 21–202（182 aa）
Mutation：C40G CIT CITRIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；277 K；PEG 4000, sodium citrate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.20 Å R-free 0.229
2RD7 Human Complement Membrane Attack Proteins Share a Common Fold with Bacterial Cytolysins Deposited 2007-09-21 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 21–202（182 aa）
Not recorded CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5；289 K；6% PEG 20,000, 0.1 M citric acid, 0.025 M Mg(NO3)2, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 2.15 Å R-free 0.261
3OJY Crystal Structure of Human Complement Component C8 Deposited 2010-08-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 21–202（182 aa） Fragment：unp residues 21-202
Not recorded CA CALCIUM ION × 2 BMA beta-D-mannopyranose × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.4；281 K；14-16% PEG 4K, 100mM TAPS pH 9.0, 400mM NaCl, 10mM SrCl2, VAPOR DIFFUSION, HANGING DROP, temperature 281K
Resolution 2.51 Å R-free 0.337
6H04 Closed conformation of the Membrane Attack Complex Deposited 2018-07-06 Different oligomeric state Different ligand/ion Assembly 1 Other combination Heteromer；Protein × 24 PDB declaration: 24-meric
Chain E 21–202（182 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 38 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.60 Å
7NYC cryoEM structure of 3C9-sMAC Deposited 2021-03-22 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain F 21–202（182 aa）
Not recorded MAN alpha-D-mannopyranose × 7 CA CALCIUM ION × 3 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 FUC alpha-L-fucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.54 Å
7NYD cryoEM structure of 2C9-sMAC Deposited 2021-03-22 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 8 PDB declaration: octameric
Chain F 21–202（182 aa）
Not recorded MAN alpha-D-mannopyranose × 6 CA CALCIUM ION × 4 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 FUC alpha-L-fucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.27 Å
8B0F CryoEM structure of C5b8-CD59 Deposited 2022-09-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain F 1–202（202 aa）
Not recorded CA CALCIUM ION × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8B0G 2C9, C5b9-CD59 structure Deposited 2022-09-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 10 PDB declaration: decameric
Chain F 1–202（202 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20 mM HEPES pH 7.4, 120 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8B0H 2C9, C5b9-CD59 cryoEM structure Deposited 2022-09-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric
Chain F 1–202（202 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20 mM HEPES pH 7.4, 120 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å

10 other PDB entries and 10 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name CO8G_HUMAN
Isoform —
PDB entities 4
Chains and sequence ranges Author chain E； PDBConstruct 1–182； UniProt 21–202

Complement component C8 alpha chain

OrganismNot specified

UniProt P07357

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Other combination Heteromer Protein × 24 其他Polymer 21 PDB declaration: 24-meric（24） Consistent with protein copy count Chain F； UniProt 31–584 Not recorded Complement C5,Complement C5 × 1 （P01031） Complement component C8 beta chain × 1 （P07358） Complement component C7 × 1 （P10643） Complement component C8 gamma chain × 1 （P07360） Complement component C6 × 1 （P13671） Complement component C9 × 18 （P02748） beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 1 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 20 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 37 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 5.60 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 6H03 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
2QOS Crystal structure of complement protein C8 in complex with a peptide containing the C8 binding site on C8 Deposited 2007-07-20 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 188–198（11 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；26-28% PEG6K, 0.1M Tris pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 1.81 Å R-free 0.244
2QQH Structure of C8a-MACPF reveals mechanism of membrane attack in complement immune defense Deposited 2007-07-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 133–366（234 aa）
Chain A 410–492（83 aa）
Mutation：C164S Mutation：C164S SO4 SULFATE ION × 3 NI NICKEL (II) ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；303 K；1M LiSO4, 5mM NiCl2, 100mM Tris, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 303K
Resolution 2.50 Å R-free 0.287
2RD7 Human Complement Membrane Attack Proteins Share a Common Fold with Bacterial Cytolysins Deposited 2007-09-21 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 133–492（360 aa）
Not recorded CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5；289 K；6% PEG 20,000, 0.1 M citric acid, 0.025 M Mg(NO3)2, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 2.15 Å R-free 0.261
3OJY Crystal Structure of Human Complement Component C8 Deposited 2010-08-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 31–584（554 aa） Fragment：unp residues 31-584
Not recorded CA CALCIUM ION × 2 BMA beta-D-mannopyranose × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.4；281 K；14-16% PEG 4K, 100mM TAPS pH 9.0, 400mM NaCl, 10mM SrCl2, VAPOR DIFFUSION, HANGING DROP, temperature 281K
Resolution 2.51 Å R-free 0.337
6H04 Closed conformation of the Membrane Attack Complex Deposited 2018-07-06 Different oligomeric state Different ligand/ion Assembly 1 Other combination Heteromer；Protein × 24 PDB declaration: 24-meric
Chain F 31–584（554 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 38 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.60 Å
7NYC cryoEM structure of 3C9-sMAC Deposited 2021-03-22 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain E 31–584（554 aa）
Not recorded MAN alpha-D-mannopyranose × 7 CA CALCIUM ION × 3 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 FUC alpha-L-fucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.54 Å
7NYD cryoEM structure of 2C9-sMAC Deposited 2021-03-22 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 8 PDB declaration: octameric
Chain E 31–584（554 aa）
Not recorded MAN alpha-D-mannopyranose × 6 CA CALCIUM ION × 4 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 FUC alpha-L-fucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.27 Å
8B0F CryoEM structure of C5b8-CD59 Deposited 2022-09-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain E 1–584（584 aa）
Not recorded CA CALCIUM ION × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8B0G 2C9, C5b9-CD59 structure Deposited 2022-09-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 10 PDB declaration: decameric
Chain E 1–584（584 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20 mM HEPES pH 7.4, 120 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8B0H 2C9, C5b9-CD59 cryoEM structure Deposited 2022-09-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric
Chain E 1–584（584 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20 mM HEPES pH 7.4, 120 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å

10 other PDB entries and 10 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name CO8A_HUMAN
Isoform —
PDB entities 5
Chains and sequence ranges Author chain F； PDBConstruct 1–554； UniProt 31–584

Complement component C6

OrganismNot specified

UniProt P13671

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Other combination Heteromer Protein × 24 其他Polymer 21 PDB declaration: 24-meric（24） Consistent with protein copy count Chain B； UniProt 22–934 Not recorded Complement C5,Complement C5 × 1 （P01031） Complement component C8 beta chain × 1 （P07358） Complement component C7 × 1 （P10643） Complement component C8 gamma chain × 1 （P07360） Complement component C8 alpha chain × 1 （P07357） Complement component C9 × 18 （P02748） beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 1 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 20 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 37 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 5.60 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 6H03 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3T5O Crystal Structure of human Complement Component C6 Deposited 2011-07-27 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: monomeric
Chain A 22–934（913 aa）
Not recorded CD CADMIUM ION × 1 FUL beta-L-fucopyranose × 1 MAN alpha-D-mannopyranose × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 5.8；284 K；5 mM MES, 89 mM Sodium Chloride, 0.01 mM Cadmium Chloride, pH 5.8, EVAPORATION, temperature 284K
Resolution 2.87 Å R-free 0.276
4A5W Crystal structure of C5b6 Deposited 2011-10-28 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 22–934（913 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 CA CALCIUM ION × 1 FUC alpha-L-fucopyranose × 1 MAN alpha-D-mannopyranose × 3 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.8；167 MM HEPES-NAOH PH7.8
Resolution 3.50 Å R-free 0.270
4E0S Crystal Structure of C5b-6 Deposited 2012-03-05 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 22–934（913 aa）
Not recorded NA SODIUM ION × 1 CA CALCIUM ION × 1 MAN alpha-D-mannopyranose × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.6；298 K；0.08M lithium chloride, 0.01M imidazole-HCl, pH 7.6, SMALL TUBES, temperature 298K
Resolution 4.21 Å R-free 0.278
6H04 Closed conformation of the Membrane Attack Complex Deposited 2018-07-06 Different oligomeric state Different ligand/ion Assembly 1 Other combination Heteromer；Protein × 24 PDB declaration: 24-meric
Chain B 22–934（913 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 38 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.60 Å
7NYC cryoEM structure of 3C9-sMAC Deposited 2021-03-22 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain B 22–934（913 aa）
Not recorded MAN alpha-D-mannopyranose × 7 CA CALCIUM ION × 3 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 FUC alpha-L-fucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.54 Å
7NYD cryoEM structure of 2C9-sMAC Deposited 2021-03-22 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 8 PDB declaration: octameric
Chain B 22–934（913 aa）
Not recorded MAN alpha-D-mannopyranose × 6 CA CALCIUM ION × 4 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 FUC alpha-L-fucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.27 Å
7Q6C complement C6 FIM1-2 bound to CP010 antibody Deposited 2021-11-06 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 769–934（166 aa）
Not recorded ACT ACETATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.4；291 K；112 mM calcium acetate, 56 mM sodium cacodylate pH 6.4, 8.5% PEG8000 and 16% glycerol
Resolution 2.29 Å R-free 0.233
8B0F CryoEM structure of C5b8-CD59 Deposited 2022-09-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain B 1–934（934 aa）
Not recorded CA CALCIUM ION × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8B0G 2C9, C5b9-CD59 structure Deposited 2022-09-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 10 PDB declaration: decameric
Chain B 1–934（934 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20 mM HEPES pH 7.4, 120 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8B0H 2C9, C5b9-CD59 cryoEM structure Deposited 2022-09-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric
Chain B 1–934（934 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20 mM HEPES pH 7.4, 120 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å

10 other PDB entries and 10 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name CO6_HUMAN
Isoform —
PDB entities 6
Chains and sequence ranges Author chain B； PDBConstruct 1–913； UniProt 22–934

Complement component C9

OrganismNot specified

UniProt P02748

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Other combination Heteromer Protein × 24 其他Polymer 21 PDB declaration: 24-meric（24） Consistent with protein copy count Chain G； UniProt 22–559 Chain H； UniProt 22–559 Chain I； UniProt 22–559 Chain J； UniProt 22–559 Chain K； UniProt 22–559 Chain L； UniProt 22–559 Chain M； UniProt 22–559 Chain N； UniProt 22–559 Chain O； UniProt 22–559 Chain P； UniProt 22–559 Chain Q； UniProt 22–559 Chain R； UniProt 22–559 Chain S； UniProt 22–559 Chain T； UniProt 22–559 Chain U； UniProt 22–559 Chain V； UniProt 22–559 Chain W； UniProt 22–559 Chain X； UniProt 22–559 Not recorded Complement C5,Complement C5 × 1 （P01031） Complement component C8 beta chain × 1 （P07358） Complement component C7 × 1 （P10643） Complement component C8 gamma chain × 1 （P07360） Complement component C8 alpha chain × 1 （P07357） Complement component C6 × 1 （P13671） beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 1 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 20 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 37 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 5.60 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 6H03 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5FMW The poly-C9 component of the Complement Membrane Attack Complex Deposited 2015-11-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 22 PDB declaration: 22-meric
Chain A 39–544（506 aa）
Chain B 39–544（506 aa）
Chain C 39–544（506 aa）
Chain D 39–544（506 aa）
Chain E 39–544（506 aa）
Chain F 39–544（506 aa）
Chain G 39–544（506 aa）
Chain H 39–544（506 aa）
Chain I 39–544（506 aa）
Chain J 39–544（506 aa）
Chain K 39–544（506 aa）
Chain L 39–544（506 aa）
Chain M 39–544（506 aa）
Chain N 39–544（506 aa）
Chain O 39–544（506 aa）
Chain P 39–544（506 aa）
Chain Q 39–544（506 aa）
Chain R 39–544（506 aa）
Chain S 39–544（506 aa）
Chain T 39–544（506 aa）
Chain U 39–544（506 aa）
Chain V 39–544（506 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 10 MM TRIS, 50 MM NACL；pH 8；10 MM TRIS, 50 MM NACL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 6.70 Å
6DLW Complement component polyC9 Deposited 2018-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 22 PDB declaration: 22-meric
Chain A 22–559（538 aa）
Chain B 22–559（538 aa）
Chain C 22–559（538 aa）
Chain D 22–559（538 aa）
Chain E 22–559（538 aa）
Chain F 22–559（538 aa）
Chain G 22–559（538 aa）
Chain H 22–559（538 aa）
Chain I 22–559（538 aa）
Chain J 22–559（538 aa）
Chain K 22–559（538 aa）
Chain L 22–559（538 aa）
Chain M 22–559（538 aa）
Chain N 22–559（538 aa）
Chain O 22–559（538 aa）
Chain P 22–559（538 aa）
Chain Q 22–559（538 aa）
Chain R 22–559（538 aa）
Chain S 22–559（538 aa）
Chain T 22–559（538 aa）
Chain U 22–559（538 aa）
Chain V 22–559（538 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 22 BMA beta-D-mannopyranose × 22 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 2 seconds before plunging
Resolution 3.90 Å
6H04 Closed conformation of the Membrane Attack Complex Deposited 2018-07-06 Different oligomeric state Different ligand/ion Assembly 1 Other combination Heteromer；Protein × 24 PDB declaration: 24-meric
Chain G 22–559（538 aa）
Chain H 22–559（538 aa）
Chain I 22–559（538 aa）
Chain J 22–559（538 aa）
Chain K 22–559（538 aa）
Chain L 22–559（538 aa）
Chain M 22–559（538 aa）
Chain N 22–559（538 aa）
Chain O 22–559（538 aa）
Chain P 22–559（538 aa）
Chain Q 22–559（538 aa）
Chain R 22–559（538 aa）
Chain S 22–559（538 aa）
Chain T 22–559（538 aa）
Chain U 22–559（538 aa）
Chain V 22–559（538 aa）
Chain W 22–559（538 aa）
Chain X 22–559（538 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 38 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.60 Å
7NYC cryoEM structure of 3C9-sMAC Deposited 2021-03-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain G 22–559（538 aa）
Chain H 22–559（538 aa）
Chain I 22–559（538 aa）
Not recorded MAN alpha-D-mannopyranose × 7 CA CALCIUM ION × 3 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 FUC alpha-L-fucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.54 Å
7NYD cryoEM structure of 2C9-sMAC Deposited 2021-03-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 8 PDB declaration: octameric
Chain G 22–559（538 aa）
Chain H 22–559（538 aa）
Not recorded MAN alpha-D-mannopyranose × 6 CA CALCIUM ION × 4 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 FUC alpha-L-fucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.27 Å
8B0G 2C9, C5b9-CD59 structure Deposited 2022-09-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 10 PDB declaration: decameric
Chain H 1–559（559 aa）
Chain I 1–559（559 aa）
Chain J 1–559（559 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20 mM HEPES pH 7.4, 120 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8B0H 2C9, C5b9-CD59 cryoEM structure Deposited 2022-09-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric
Chain H 1–559（559 aa）
Chain I 1–559（559 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20 mM HEPES pH 7.4, 120 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8DE6 Oligomeric C9 in complex with aE11 Fab Deposited 2022-06-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain A 21–559（539 aa）
Chain C 21–559（539 aa）
Chain G 21–559（539 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 BMA beta-D-mannopyranose × 6 CA CALCIUM ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å

8 other PDB entries and 8 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name CO9_HUMAN
Isoform —
PDB entities 7
Chains and sequence ranges Author chain G； PDBConstruct 1–538； UniProt 22–559 Author chain H； PDBConstruct 1–538； UniProt 22–559 Author chain I； PDBConstruct 1–538； UniProt 22–559 Author chain J； PDBConstruct 1–538； UniProt 22–559 Author chain K； PDBConstruct 1–538； UniProt 22–559 Author chain L； PDBConstruct 1–538； UniProt 22–559 Author chain M； PDBConstruct 1–538； UniProt 22–559 Author chain N； PDBConstruct 1–538； UniProt 22–559 Author chain O； PDBConstruct 1–538； UniProt 22–559 Author chain P； PDBConstruct 1–538； UniProt 22–559 Author chain Q； PDBConstruct 1–538； UniProt 22–559 Author chain R； PDBConstruct 1–538； UniProt 22–559 Author chain S； PDBConstruct 1–538； UniProt 22–559 Author chain T； PDBConstruct 1–538； UniProt 22–559 Author chain U； PDBConstruct 1–538； UniProt 22–559 Author chain V； PDBConstruct 1–538； UniProt 22–559 Author chain W； PDBConstruct 1–538； UniProt 22–559 Author chain X； PDBConstruct 1–538； UniProt 22–559

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 6h03

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 6h03
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2. Structure Basics 2. Structure Basics

Entry ID entry_id6h03
Deposition date deposition_date2018-07-06
Structure title titleOPEN CONFORMATION OF THE MEMBRANE ATTACK COMPLEX
Keywords keywordsC5B9, IMMUNE SYSTEM; IMMUNE SYSTEM
Experimental Method methodELECTRON MICROSCOPY
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier— Å
Radius of gyration Rg (electron density) rg_electron99.85 Å
Forward intensity I(0) i030847100000.00
Molecular weight molecular_weight1458900.0 kDa
Excluded volume excluded_volume1807700 ų
Envelope volume envelope_volume4021000 ų
Hydration-shell volume shell_volume331260 ų
Envelope diameter envelope_diameter332.8 Å
Shell Rg shell_rg108.40 Å
Envelope Rg envelope_rg91.44 Å
Shape Rg shape_rg99.84 Å
Total Rg total_rg99.95 Å
Total atoms total_atoms102309
Residues n_residues12776
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax247.5 Å
Rg (real space) rg_real97.99 Å
Rg uncertainty (real space) rg_real_error0.42 Å
I(0) (real space) i0_real2.9520e+10
I(0) uncertainty (real space) i0_real_error4.2600e+08
Rg (reciprocal space) rg_reciprocal103.70 Å
I(0) (reciprocal space) i0_reciprocal31170000000.0000
Solution quality estimate total_estimate0.9057
Solution quality rating solution_quality EXCELLENT a EXCELLENT solution
P(r) peaks n_peaks1
Primary peak position r_peak_primary154.7 Å
Skewness Skewness skewness-0.209
Kurtosis Kurtosis kurtosis-0.747
Angular range angular_range— – 0.0800 Å−1
Current regularization parameter α current_alpha1.6540
Highest regularization parameter α highest_alpha996500000.0000
Real-space data points n_real_points17
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.999; Stabil: 0.949; Sysdev: 1.000; Positv: 1.000; Valcen: 0.951; Smooth: 0.000
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (10)

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8. Citations (1)

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9. Files and Curves (10)