6mzf

Structural Basis of Tubulin Recruitment and Assembly by Microtubule Polymerases with Tumor Overexpressed Gene (TOG) Domain Arrays

Method: X-RAY DIFFRACTION

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Tubulin alpha-1A chain

OrganismNot specified

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 14	Tubulin beta chain × 4 （P02554） Protein Stu2p/Alp14p × 2 Designed ankyrin repeat protein (DARPIN) D1 × 4 GUANOSINE-5'-TRIPHOSPHATE × 4 MAGNESIUM ION × 8 GUANOSINE-5'-DIPHOSPHATE × 4	Consistent with protein count
2	Protein heterocomplex	Heteromer	Protein 14	Tubulin beta chain × 4 （P02554） Protein Stu2p/Alp14p × 2 Designed ankyrin repeat protein (DARPIN) D1 × 4 GUANOSINE-5'-TRIPHOSPHATE × 4 MAGNESIUM ION × 8 GUANOSINE-5'-DIPHOSPHATE × 4	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	TBA1A_PIG
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–451； UniProt 1–451 Author chain C； PDBConstruct 1–451； UniProt 1–451 Author chain H； PDBConstruct 1–451； UniProt 1–451 Author chain J； PDBConstruct 1–451； UniProt 1–451 Author chain O； PDBConstruct 1–451； UniProt 1–451 Author chain Q； PDBConstruct 1–451； UniProt 1–451 Author chain V； PDBConstruct 1–451； UniProt 1–451 Author chain X； PDBConstruct 1–451； UniProt 1–451

Tubulin beta chain

OrganismNot specified

UniProt P02554

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 14	Tubulin alpha-1A chain × 4 （P02550） Protein Stu2p/Alp14p × 2 Designed ankyrin repeat protein (DARPIN) D1 × 4 GUANOSINE-5'-TRIPHOSPHATE × 4 MAGNESIUM ION × 8 GUANOSINE-5'-DIPHOSPHATE × 4	Consistent with protein count
2	Protein heterocomplex	Heteromer	Protein 14	Tubulin alpha-1A chain × 4 （P02550） Protein Stu2p/Alp14p × 2 Designed ankyrin repeat protein (DARPIN) D1 × 4 GUANOSINE-5'-TRIPHOSPHATE × 4 MAGNESIUM ION × 8 GUANOSINE-5'-DIPHOSPHATE × 4	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	TBB_PIG
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain B； PDBConstruct 1–445； UniProt 1–445 Author chain D； PDBConstruct 1–445； UniProt 1–445 Author chain I； PDBConstruct 1–445； UniProt 1–445 Author chain K； PDBConstruct 1–445； UniProt 1–445 Author chain P； PDBConstruct 1–445； UniProt 1–445 Author chain R； PDBConstruct 1–445； UniProt 1–445 Author chain W； PDBConstruct 1–445； UniProt 1–445 Author chain Y； PDBConstruct 1–445； UniProt 1–445

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	6mzf
Deposition date deposition_date	2018-11-05
Structure title title	Structural Basis of Tubulin Recruitment and Assembly by Microtubule Polymerases with Tumor Overexpressed Gene (TOG) Domain Arrays
Keywords keywords	;Microtubule, Tubulin, Tubulin polymerization, Heat repeats, Microtubule polymerase, TOG arrays, and Wheel-like assembly., CELL CYCLE ;; CELL CYCLE
Experimental Method method	X-RAY DIFFRACTION

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

6mzf__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

6mzf__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

6mzf__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	74.42 Å
Rg (electron density)	73.90 Å
Total Rg	73.97 Å
Atom count	39005
Residues	4993
Excluded volume	691540 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`6mzf__assembly_1__model_1`	tetradecameric （14）	Success	4.1.3-1-20251215 (887e7ef)	View Download
2	1	`6mzf__assembly_2__model_1`	tetradecameric （14）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (7)

Entity 1 Entity 1 polymer

Description description	Tubulin alpha-1A chain
Molecular weight formula_weight	50121.3 kDa
Source method src_method	nat
Copy count entity_count	8

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,C,H,J,O,Q,V,X
Atom count atom_count	78030

MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFSVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRAHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYEPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGVDSVEGEGEEEGEEY

Entity 2 Entity 2 polymer

Description description	Tubulin beta chain
Molecular weight formula_weight	49907.8 kDa
Source method src_method	nat
Copy count entity_count	8

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	B,D,I,K,P,R,W,Y

MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEAAGNKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATADEQGEFEEEGEEDEA

Entity 3 Entity 3 polymer

Description description	Protein Stu2p/Alp14p
Molecular weight formula_weight	63099.2 kDa
Source method src_method	man
Copy count entity_count	4

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	E,L,S,Z

MADQDDVDFTTLPLEQRASHKVWKARLNAYQELNNLFTKSSVISPPNDVANYWLDPELFASYIVDSNVVAQENAIIALHTLLEYISQVPNVSTSKLRLQWIPPLVEKGLSSSRAATKAKATDCIMLLTQSDTSIQQTVNLMLPSLSNKLPRLVSSCVKCLATIIEEFGFINVSDINILLSEILEPLPKLSSHADRNVRSETMNLILQIYKWFGKELLQELLLEKLKPIQQRDLSRMFEKYEGTIPPKQQPRLFQWQKEQEQEQEQILQTDKDGDTLMGNLLAYQDTNASAIHPATKPAVDPFELLPPSVILDKFPADFQTRISSTKWKDRVEALEEIHNNVLKPVKKLAHKNQDYSDYLRVLANVIQKDANVQAVTIAANSVQLLCNSLRSNFTRSYGAIVLVPLLERTKEKKPSVNEAICSALDAVATYCGFDDCLEETLNYMKHKTPQVRIECTKFLTRMLQGWKSDGPLQNQLLFKLLPEVTTAVLKIVNDTQPTTRNTGFECFATLMKLVGERELADPLEKLDNLKKKKIYEYYEKVEVATGLEHHHHHH

Source Source

Source organism organism	Lachancea kluyveri NRRL Y-12651
NCBI taxonomy ID ncbi_taxonomy_id	226302
Host organism host_organism	Escherichia coli

Entity 4 Entity 4 polymer

Description description	Designed ankyrin repeat protein (DARPIN) D1
Molecular weight formula_weight	18068.4 kDa
Source method src_method	man
Copy count entity_count	8

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	F,G,M,N,T,U,a,b

MRGSHHHHHHGSDLGKKLLEAARAGQDDEVRILMANGADVNATDASGLTPLHLAATYGHLEIVEVLLKHGADVNAIDIMGSTPLHLAALIGHLEIVEVLLKHGADVNAVDTWGDTPLHLAAIMGHLEIVEVLLKHGADVNAQDKFGKTAFDISIDNGNEDLAEILQKLN

Source Source

Source organism organism	Escherichia coli
NCBI taxonomy ID ncbi_taxonomy_id	562
Host organism host_organism	Escherichia coli

Entity 5 Entity 5 non-polymer

Description description	GUANOSINE-5'-TRIPHOSPHATE
Molecular weight formula_weight	523.2 kDa
Source method src_method	syn
Copy count entity_count	8

Entity 6 Entity 6 non-polymer

Description description	MAGNESIUM ION
Molecular weight formula_weight	24.3 kDa
Source method src_method	syn
Copy count entity_count	16

Entity 7 Entity 7 non-polymer

Description description	GUANOSINE-5'-DIPHOSPHATE
Molecular weight formula_weight	443.2 kDa
Source method src_method	syn
Copy count entity_count	8

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Unit-cell length a length_a	218.800 Å
Unit-cell length b length_b	107.650 Å
Unit-cell length c length_c	282.740 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.38 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	16

Space group space_group	P 1 21 1
Space-group number int_tables_number	4
Crystal system crystal_system	—

Resolution resolution	4.400 Å
Low-resolution limit resolution_low	96.592 Å
R factor r_factor	0.2331
R_work r_work	0.2247
R_free r_free	0.2515

Structural Basis of Tubulin Recruitment and Assembly by Microtubule Polymerases with Tumor Overexpressed Gene (TOG) Domain Arrays

1. Protein Identity and Related Structures Protein Identity & Related Structures

Tubulin alpha-1A chain

State in the Current Structure

Other States of the Same Protein in the Database

Tubulin beta chain

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

6mzf__assembly_1__model_1

6mzf__assembly_1__model_1 | I(q)

6mzf__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (7)

Entity 1 Entity 1 polymer

Polymer Polymer

Entity 2 Entity 2 polymer

Polymer Polymer

Entity 3 Entity 3 polymer

Polymer Polymer

Source Source

Entity 4 Entity 4 polymer

Polymer Polymer

Source Source

Entity 5 Entity 5 non-polymer

Entity 6 Entity 6 non-polymer

Entity 7 Entity 7 non-polymer

7. Citations (1)

Structural Basis of Tubulin Recruitment and Assembly by Microtubule Polymerases with Tumor Overexpressed Gene (TOG) Domain Arrays

1. Protein Identity and Related Structures Protein Identity & Related Structures

Tubulin alpha-1A chain

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Tubulin beta chain

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

6mzf__assembly_1__model_1

6mzf__assembly_1__model_1 | I(q)

6mzf__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (7)

Entity 1 Entity 1 polymer

Polymer Polymer

Entity 2 Entity 2 polymer

Polymer Polymer

Entity 3 Entity 3 polymer

Polymer Polymer

Source Source

Entity 4 Entity 4 polymer

Polymer Polymer

Source Source

Entity 5 Entity 5 non-polymer

Entity 6 Entity 6 non-polymer

Entity 7 Entity 7 non-polymer

7. Citations (1)