NADH-ubiquinone oxidoreductase chain 2
OrganismNot specified
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count | Chain 2； UniProt 1–347 | Not recorded | NADH-ubiquinone oxidoreductase chain 3 × 1 （P03898） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03910） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03902） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03924） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P25708） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P04394） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P15690） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （P17694） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （P23709） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （P42026） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （P42028） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P25712） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q02375） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q02379） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P23934） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （Q02377） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q02370） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q02371） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （P23935） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q02366） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q05752） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P42029） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （P34943） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （P34942） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q8HXG6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （O97725） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q95KV7） Acyl carrier protein, mitochondrial × 2 （P52505） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （Q02378） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q02374） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q02365） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （P48305） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q02380） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q02367） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q02368） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q02369） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q8HXG5） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q02376） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q02827） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03887） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03920） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q02373） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q02372） 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 | ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE | Resolution 3.80 Å |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 7DGZ | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 5LC5 Structure of mammalian respiratory Complex I, class2 Deposited 2016-06-19 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric |
Chain N
2–345（344 aa）
|
Not recorded | SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
|
Resolution 4.35 Å |
| 5LDW Structure of mammalian respiratory Complex I, class1 Deposited 2016-06-28 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric |
Chain N
1–347（347 aa）
|
Not recorded | SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8；20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7
cryo-EM vitrification conditions
Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus present in a cold room, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
|
Resolution 4.27 Å |
| 5LDX Structure of mammalian respiratory Complex I, class3. Deposited 2016-06-28 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric |
Chain N
1–347（347 aa）
|
Not recorded | SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7；pH 8
cryo-EM vitrification conditions
Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
|
Resolution 5.60 Å |
| 5O31 Mitochondrial complex I in the deactive state Deposited 2017-05-23 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric |
Chain N
1–347（347 aa）
|
Not recorded | SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.55
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.13 Å |
| 7DGQ Activity optimized supercomplex state1 Deposited 2020-11-12 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric |
Chain 2
1–347（347 aa）
|
Not recorded | PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 CU COPPER (II) ION × 3 HEA HEME-A × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 UQ2 UBIQUINONE-2 × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.00 Å |
| 7DGR Activity optimized supercomplex state2 Deposited 2020-11-12 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric |
Chain 2
1–347（347 aa）
|
Not recorded | FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 3 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.60 Å |
| 7DGS Activity optimized supercomplex state3 Deposited 2020-11-12 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric |
Chain 2
1–347（347 aa）
|
Not recorded | FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.80 Å |
| 7DH0 Activity optimized complex I (open form) Deposited 2020-11-12 | Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric |
Chain 2
1–347（347 aa）
|
Not recorded | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.20 Å |
| 7DKF Activity optimized supercomplex state4 Deposited 2020-11-24 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric |
Chain 22
1–347（347 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 8.30 Å |
| 7QSD Bovine complex I in the active state at 3.1 A Deposited 2022-01-13 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric |
Chain N
1–347（347 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 4 LMT DODECYL-BETA-D-MALTOSIDE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.14
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 7QSK Bovine complex I in lipid nanodisc, Active-Q10 Deposited 2022-01-13 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric |
Chain N
1–347（347 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；blot for 10 seconds before plunging
|
Resolution 2.84 Å |
| 7QSL Bovine complex I in lipid nanodisc, Active-apo Deposited 2022-01-13 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric |
Chain N
1–347（347 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；blot for 10 seconds before plunging
|
Resolution 2.76 Å |
| 7QSM Bovine complex I in lipid nanodisc, Deactive-ligand (composite) Deposited 2022-01-13 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric |
Chain N
1–347（347 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 GOL GLYCEROL × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；blot for 10 seconds before plunging
|
Resolution 2.30 Å |
| 7QSN Bovine complex I in lipid nanodisc, Deactive-apo Deposited 2022-01-13 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric |
Chain N
1–347（347 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；blot for 10 seconds before plunging
|
Resolution 2.81 Å |
| 7QSO Bovine complex I in lipid nanodisc, State 3 (Slack) Deposited 2022-01-13 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric |
Chain N
1–347（347 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 2 CDL CARDIOLIPIN × 6 U10 UBIQUINONE-10 × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；blot for 10 seconds before plunging
|
Resolution 3.02 Å |
| 7R41 Bovine complex I in the presence of IM1761092, active class i (Composite map) Deposited 2022-02-08 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric |
Chain N
1–347（347 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.14
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.30 Å |
| 7R42 Bovine complex I in the presence of IM1761092, active class ii (Composite map) Deposited 2022-02-08 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric |
Chain N
1–347（347 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | LMT DODECYL-BETA-D-MALTOSIDE × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.14
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.30 Å |
| 7R43 Bovine complex I in the presence of IM1761092, active class iii (Composite map) Deposited 2022-02-08 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric |
Chain N
1–347（347 aa）
|
Mutation：N278K Non-standard monomer：Yes (specific site not provided by mmCIF) | LMT DODECYL-BETA-D-MALTOSIDE × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 CDL CARDIOLIPIN × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.14
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.40 Å |
| 7R44 Bovine complex I in the presence of IM1761092, active class iv (Composite map) Deposited 2022-02-08 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric |
Chain N
1–347（347 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | LMT DODECYL-BETA-D-MALTOSIDE × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.14
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.40 Å |
| 7R45 Bovine complex I in the presence of IM1761092, deactive class i (Composite map) Deposited 2022-02-08 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric |
Chain N
1–347（347 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.14
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.40 Å |
| 7R46 Bovine complex I in the presence of IM1761092, deactive class ii (Composite map) Deposited 2022-02-08 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric |
Chain N
1–347（347 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | LMT DODECYL-BETA-D-MALTOSIDE × 11 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.14
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.40 Å |
| 7R47 Bovine complex I in the presence of IM1761092, deactive class iii (Composite map) Deposited 2022-02-08 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric |
Chain N
1–347（347 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.14
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.30 Å |
| 7R48 Bovine complex I in the presence of IM1761092, deactive class iv (Composite map) Deposited 2022-02-08 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric |
Chain N
1–347（347 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | LMT DODECYL-BETA-D-MALTOSIDE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.14
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.30 Å |
| 7R4C Bovine complex I in the presence of IM1761092, deactive class v (Composite map) Deposited 2022-02-08 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric |
Chain N
1–347（347 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | LMT DODECYL-BETA-D-MALTOSIDE × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.14
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.30 Å |
| 7R4D Bovine complex I in the presence of IM1761092, deactive class vi (Composite map) Deposited 2022-02-08 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric |
Chain N
1–347（347 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.14
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.30 Å |
| 7R4F Bovine complex I in the presence of IM1761092, slack class i (Composite map) Deposited 2022-02-08 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric |
Chain N
1–347（347 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | LMT DODECYL-BETA-D-MALTOSIDE × 7 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 CDL CARDIOLIPIN × 6 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.14
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.40 Å |
| 7R4G Bovine complex I in the presence of IM1761092, slack class ii (Composite map) Deposited 2022-02-08 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric |
Chain N
1–347（347 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 10 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.14
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.50 Å |
| 8Q0A Inward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric |
Chain N
1–347（347 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 8 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
|
Resolution 3.10 Å |
| 8Q0F Inward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric |
Chain N
1–347（347 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 21 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
|
Resolution 3.10 Å |
| 8Q0J Inward-facing, slack proteoliposome complex I at 3.8 A. Initially purified in DDM. Deposited 2023-07-28 | Different mutation/modification Different ligand/ion Different experimental conditions | Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric |
Chain N
1–347（347 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
|
Resolution 3.80 Å |
| 8Q0M Outward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric |
Chain N
1–347（347 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
|
Resolution 3.10 Å |
| 8Q0O Outward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric |
Chain N
1–347（347 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
|
Resolution 3.10 Å |
| 8Q0Q Outward-facing, slack proteoliposome complex I at 3.6 A. Initially purified in DDM Deposited 2023-07-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric |
Chain N
1–347（347 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
|
Resolution 3.60 Å |
| 8Q1P Inward-facing, open2 proteoliposome complex I at 2.9 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric |
Chain N
1–347（347 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
|
Resolution 2.90 Å |
| 8Q1U Inward-facing, open1 proteoliposome complex I at 3.3 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric |
Chain N
1–347（347 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
|
Resolution 3.30 Å |
| 8Q1Y Outward-facing, open2 proteoliposome complex I at 2.6 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric |
Chain N
1–347（347 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
|
Resolution 2.60 Å |
| 8Q25 Outward-facing, open1 proteoliposome complex I at 2.8 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric |
Chain N
1–347（347 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
|
Resolution 2.80 Å |
| 8Q45 Inward-facing, closed proteoliposome complex I at 2.7 A. Initially purified in LMNG. Deposited 2023-08-05 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric |
Chain N
1–347（347 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
|
Resolution 2.70 Å |
| 8Q46 Inward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric |
Chain N
1–347（347 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
|
Resolution 2.60 Å |
| 8Q47 Inward-facing, open1 proteoliposome complex I at 2.9 A. Initially purified in LMNG. Deposited 2023-08-05 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric |
Chain N
1–347（347 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 26 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
|
Resolution 2.90 Å |
| 8Q48 Outward-facing, closed proteoliposome complex I at 2.5 A. Initially purified in LMNG. Deposited 2023-08-05 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric |
Chain N
1–347（347 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 35 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
|
Resolution 2.50 Å |
| 8Q49 Outward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric |
Chain N
1–347（347 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 FVH [[(2~{R},3~{S},4~{R},5~{R})-5-[(3~{R})-3-aminocarbonylpiperidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methyl hydrogen phosphate × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
|
Resolution 2.60 Å |
| 8Q4A Outward-facing, open1 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric |
Chain N
1–347（347 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
|
Resolution 2.60 Å |
| 9SMF Reduced bovine complex I in lipid nanodisc, NADH-active-Q10 Deposited 2025-09-08 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric |
Chain N
1–347（347 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
|
Resolution 2.51 Å |
| 9SMG Reduced bovine complex I in lipid nanodisc, NADH-active-altQ10 Deposited 2025-09-08 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric |
Chain N
1–347（347 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
|
Resolution 2.42 Å |
| 9SMH Reduced bovine complex I in lipid nanodisc, NADH-active-DDM Deposited 2025-09-08 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric |
Chain N
1–347（347 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
|
Resolution 2.61 Å |
| 9SMI Reduced bovine complex I in lipid nanodisc, NADH-deactive Deposited 2025-09-08 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric |
Chain N
1–347（347 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 27 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 LMT DODECYL-BETA-D-MALTOSIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 CHD CHOLIC ACID × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
|
Resolution 2.01 Å |
| 9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric |
Chain N
2–347（346 aa）
|
Not recorded | FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.60 Å |
| 9W2V Cryo-EM structure of complex I on the bovine heart submitochondrial particles, closed Deposited 2025-07-28 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric |
Chain N
2–347（346 aa）
|
Not recorded | FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
49 other PDB entries and 49 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | NU2M_BOVIN |
| Isoform | — |
| PDB entities | 1 |
| Chains and sequence ranges | Author chain 2； PDBConstruct 1–347； UniProt 1–347 |
