8cgu

Gentamicin bound to the 30S body

Method: ELECTRON MICROSCOPY

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Small ribosomal subunit protein uS4

OrganismNot specified

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 12 RNA 2	16S rRNA × 1 Small ribosomal subunit protein uS5 × 1 （P0A7W1） Small ribosomal subunit protein bS6, non-modified isoform × 1 （P02358） Small ribosomal subunit protein uS8 × 1 （P0A7W7） Small ribosomal subunit protein uS11 × 1 （P0A7R9） Small ribosomal subunit protein uS12 × 1 （P0A7S3） Small ribosomal subunit protein uS15 × 1 （P0ADZ4） 30S ribosomal protein S16 × 1 （P0A7T3） Small ribosomal subunit protein uS17 × 1 （P0AG63） Small ribosomal subunit protein bS18 × 1 （P0A7T7） 30S ribosomal protein S20 × 1 （P0A7U7） Small ribosomal subunit protein bS21 × 1 （P68679） 23S rRNA × 1 POTASSIUM ION × 24 ;(2R,3R,4R,5R)-2-((1S,2S,3R,4S,6R)-4,6-DIAMINO-3-((2R,3R,6S)-3-AMINO-6-(AMINOMETHYL)-TETRAHYDRO-2H-PYRAN-2-YLOXY)-2-HYDR OXYCYCLOHEXYLOXY)-5-METHYL-4-(METHYLAMINO)-TETRAHYDRO-2H-PYRAN-3,5-DIOL ; × 4 MAGNESIUM ION × 58 water × 14	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS4_ECOLI
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain D； PDBConstruct 1–206； UniProt 1–206

Small ribosomal subunit protein uS5

OrganismNot specified

UniProt P0A7W1

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 12 RNA 2	16S rRNA × 1 Small ribosomal subunit protein uS4 × 1 （P0A7V8） Small ribosomal subunit protein bS6, non-modified isoform × 1 （P02358） Small ribosomal subunit protein uS8 × 1 （P0A7W7） Small ribosomal subunit protein uS11 × 1 （P0A7R9） Small ribosomal subunit protein uS12 × 1 （P0A7S3） Small ribosomal subunit protein uS15 × 1 （P0ADZ4） 30S ribosomal protein S16 × 1 （P0A7T3） Small ribosomal subunit protein uS17 × 1 （P0AG63） Small ribosomal subunit protein bS18 × 1 （P0A7T7） 30S ribosomal protein S20 × 1 （P0A7U7） Small ribosomal subunit protein bS21 × 1 （P68679） 23S rRNA × 1 POTASSIUM ION × 24 ;(2R,3R,4R,5R)-2-((1S,2S,3R,4S,6R)-4,6-DIAMINO-3-((2R,3R,6S)-3-AMINO-6-(AMINOMETHYL)-TETRAHYDRO-2H-PYRAN-2-YLOXY)-2-HYDR OXYCYCLOHEXYLOXY)-5-METHYL-4-(METHYLAMINO)-TETRAHYDRO-2H-PYRAN-3,5-DIOL ; × 4 MAGNESIUM ION × 58 water × 14	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS5_ECOLI
Isoform	—
PDB entities	3
Chains and sequence ranges	Author chain E； PDBConstruct 1–167； UniProt 1–167

Small ribosomal subunit protein bS6, non-modified isoform

OrganismNot specified

UniProt P02358

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 12 RNA 2	16S rRNA × 1 Small ribosomal subunit protein uS4 × 1 （P0A7V8） Small ribosomal subunit protein uS5 × 1 （P0A7W1） Small ribosomal subunit protein uS8 × 1 （P0A7W7） Small ribosomal subunit protein uS11 × 1 （P0A7R9） Small ribosomal subunit protein uS12 × 1 （P0A7S3） Small ribosomal subunit protein uS15 × 1 （P0ADZ4） 30S ribosomal protein S16 × 1 （P0A7T3） Small ribosomal subunit protein uS17 × 1 （P0AG63） Small ribosomal subunit protein bS18 × 1 （P0A7T7） 30S ribosomal protein S20 × 1 （P0A7U7） Small ribosomal subunit protein bS21 × 1 （P68679） 23S rRNA × 1 POTASSIUM ION × 24 ;(2R,3R,4R,5R)-2-((1S,2S,3R,4S,6R)-4,6-DIAMINO-3-((2R,3R,6S)-3-AMINO-6-(AMINOMETHYL)-TETRAHYDRO-2H-PYRAN-2-YLOXY)-2-HYDR OXYCYCLOHEXYLOXY)-5-METHYL-4-(METHYLAMINO)-TETRAHYDRO-2H-PYRAN-3,5-DIOL ; × 4 MAGNESIUM ION × 58 water × 14	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS6_ECOLI
Isoform	—
PDB entities	4
Chains and sequence ranges	Author chain F； PDBConstruct 1–131； UniProt 1–131

Small ribosomal subunit protein uS8

OrganismNot specified

UniProt P0A7W7

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 12 RNA 2	16S rRNA × 1 Small ribosomal subunit protein uS4 × 1 （P0A7V8） Small ribosomal subunit protein uS5 × 1 （P0A7W1） Small ribosomal subunit protein bS6, non-modified isoform × 1 （P02358） Small ribosomal subunit protein uS11 × 1 （P0A7R9） Small ribosomal subunit protein uS12 × 1 （P0A7S3） Small ribosomal subunit protein uS15 × 1 （P0ADZ4） 30S ribosomal protein S16 × 1 （P0A7T3） Small ribosomal subunit protein uS17 × 1 （P0AG63） Small ribosomal subunit protein bS18 × 1 （P0A7T7） 30S ribosomal protein S20 × 1 （P0A7U7） Small ribosomal subunit protein bS21 × 1 （P68679） 23S rRNA × 1 POTASSIUM ION × 24 ;(2R,3R,4R,5R)-2-((1S,2S,3R,4S,6R)-4,6-DIAMINO-3-((2R,3R,6S)-3-AMINO-6-(AMINOMETHYL)-TETRAHYDRO-2H-PYRAN-2-YLOXY)-2-HYDR OXYCYCLOHEXYLOXY)-5-METHYL-4-(METHYLAMINO)-TETRAHYDRO-2H-PYRAN-3,5-DIOL ; × 4 MAGNESIUM ION × 58 water × 14	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS8_ECOLI
Isoform	—
PDB entities	5
Chains and sequence ranges	Author chain H； PDBConstruct 1–130； UniProt 1–130

Small ribosomal subunit protein uS11

OrganismNot specified

UniProt P0A7R9

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 12 RNA 2	16S rRNA × 1 Small ribosomal subunit protein uS4 × 1 （P0A7V8） Small ribosomal subunit protein uS5 × 1 （P0A7W1） Small ribosomal subunit protein bS6, non-modified isoform × 1 （P02358） Small ribosomal subunit protein uS8 × 1 （P0A7W7） Small ribosomal subunit protein uS12 × 1 （P0A7S3） Small ribosomal subunit protein uS15 × 1 （P0ADZ4） 30S ribosomal protein S16 × 1 （P0A7T3） Small ribosomal subunit protein uS17 × 1 （P0AG63） Small ribosomal subunit protein bS18 × 1 （P0A7T7） 30S ribosomal protein S20 × 1 （P0A7U7） Small ribosomal subunit protein bS21 × 1 （P68679） 23S rRNA × 1 POTASSIUM ION × 24 ;(2R,3R,4R,5R)-2-((1S,2S,3R,4S,6R)-4,6-DIAMINO-3-((2R,3R,6S)-3-AMINO-6-(AMINOMETHYL)-TETRAHYDRO-2H-PYRAN-2-YLOXY)-2-HYDR OXYCYCLOHEXYLOXY)-5-METHYL-4-(METHYLAMINO)-TETRAHYDRO-2H-PYRAN-3,5-DIOL ; × 4 MAGNESIUM ION × 58 water × 14	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS11_ECOLI
Isoform	—
PDB entities	6
Chains and sequence ranges	Author chain K； PDBConstruct 1–129； UniProt 1–129

Small ribosomal subunit protein uS12

OrganismNot specified

UniProt P0A7S3

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 12 RNA 2	16S rRNA × 1 Small ribosomal subunit protein uS4 × 1 （P0A7V8） Small ribosomal subunit protein uS5 × 1 （P0A7W1） Small ribosomal subunit protein bS6, non-modified isoform × 1 （P02358） Small ribosomal subunit protein uS8 × 1 （P0A7W7） Small ribosomal subunit protein uS11 × 1 （P0A7R9） Small ribosomal subunit protein uS15 × 1 （P0ADZ4） 30S ribosomal protein S16 × 1 （P0A7T3） Small ribosomal subunit protein uS17 × 1 （P0AG63） Small ribosomal subunit protein bS18 × 1 （P0A7T7） 30S ribosomal protein S20 × 1 （P0A7U7） Small ribosomal subunit protein bS21 × 1 （P68679） 23S rRNA × 1 POTASSIUM ION × 24 ;(2R,3R,4R,5R)-2-((1S,2S,3R,4S,6R)-4,6-DIAMINO-3-((2R,3R,6S)-3-AMINO-6-(AMINOMETHYL)-TETRAHYDRO-2H-PYRAN-2-YLOXY)-2-HYDR OXYCYCLOHEXYLOXY)-5-METHYL-4-(METHYLAMINO)-TETRAHYDRO-2H-PYRAN-3,5-DIOL ; × 4 MAGNESIUM ION × 58 water × 14	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS12_ECOLI
Isoform	—
PDB entities	7
Chains and sequence ranges	Author chain L； PDBConstruct 1–124； UniProt 1–124

Small ribosomal subunit protein uS15

OrganismNot specified

UniProt P0ADZ4

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 12 RNA 2	16S rRNA × 1 Small ribosomal subunit protein uS4 × 1 （P0A7V8） Small ribosomal subunit protein uS5 × 1 （P0A7W1） Small ribosomal subunit protein bS6, non-modified isoform × 1 （P02358） Small ribosomal subunit protein uS8 × 1 （P0A7W7） Small ribosomal subunit protein uS11 × 1 （P0A7R9） Small ribosomal subunit protein uS12 × 1 （P0A7S3） 30S ribosomal protein S16 × 1 （P0A7T3） Small ribosomal subunit protein uS17 × 1 （P0AG63） Small ribosomal subunit protein bS18 × 1 （P0A7T7） 30S ribosomal protein S20 × 1 （P0A7U7） Small ribosomal subunit protein bS21 × 1 （P68679） 23S rRNA × 1 POTASSIUM ION × 24 ;(2R,3R,4R,5R)-2-((1S,2S,3R,4S,6R)-4,6-DIAMINO-3-((2R,3R,6S)-3-AMINO-6-(AMINOMETHYL)-TETRAHYDRO-2H-PYRAN-2-YLOXY)-2-HYDR OXYCYCLOHEXYLOXY)-5-METHYL-4-(METHYLAMINO)-TETRAHYDRO-2H-PYRAN-3,5-DIOL ; × 4 MAGNESIUM ION × 58 water × 14	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS15_ECOLI
Isoform	—
PDB entities	8
Chains and sequence ranges	Author chain O； PDBConstruct 1–89； UniProt 1–89

30S ribosomal protein S16

OrganismNot specified

UniProt P0A7T3

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 12 RNA 2	16S rRNA × 1 Small ribosomal subunit protein uS4 × 1 （P0A7V8） Small ribosomal subunit protein uS5 × 1 （P0A7W1） Small ribosomal subunit protein bS6, non-modified isoform × 1 （P02358） Small ribosomal subunit protein uS8 × 1 （P0A7W7） Small ribosomal subunit protein uS11 × 1 （P0A7R9） Small ribosomal subunit protein uS12 × 1 （P0A7S3） Small ribosomal subunit protein uS15 × 1 （P0ADZ4） Small ribosomal subunit protein uS17 × 1 （P0AG63） Small ribosomal subunit protein bS18 × 1 （P0A7T7） 30S ribosomal protein S20 × 1 （P0A7U7） Small ribosomal subunit protein bS21 × 1 （P68679） 23S rRNA × 1 POTASSIUM ION × 24 ;(2R,3R,4R,5R)-2-((1S,2S,3R,4S,6R)-4,6-DIAMINO-3-((2R,3R,6S)-3-AMINO-6-(AMINOMETHYL)-TETRAHYDRO-2H-PYRAN-2-YLOXY)-2-HYDR OXYCYCLOHEXYLOXY)-5-METHYL-4-(METHYLAMINO)-TETRAHYDRO-2H-PYRAN-3,5-DIOL ; × 4 MAGNESIUM ION × 58 water × 14	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS16_ECOLI
Isoform	—
PDB entities	9
Chains and sequence ranges	Author chain P； PDBConstruct 1–82； UniProt 1–82

Small ribosomal subunit protein uS17

OrganismNot specified

UniProt P0AG63

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 12 RNA 2	16S rRNA × 1 Small ribosomal subunit protein uS4 × 1 （P0A7V8） Small ribosomal subunit protein uS5 × 1 （P0A7W1） Small ribosomal subunit protein bS6, non-modified isoform × 1 （P02358） Small ribosomal subunit protein uS8 × 1 （P0A7W7） Small ribosomal subunit protein uS11 × 1 （P0A7R9） Small ribosomal subunit protein uS12 × 1 （P0A7S3） Small ribosomal subunit protein uS15 × 1 （P0ADZ4） 30S ribosomal protein S16 × 1 （P0A7T3） Small ribosomal subunit protein bS18 × 1 （P0A7T7） 30S ribosomal protein S20 × 1 （P0A7U7） Small ribosomal subunit protein bS21 × 1 （P68679） 23S rRNA × 1 POTASSIUM ION × 24 ;(2R,3R,4R,5R)-2-((1S,2S,3R,4S,6R)-4,6-DIAMINO-3-((2R,3R,6S)-3-AMINO-6-(AMINOMETHYL)-TETRAHYDRO-2H-PYRAN-2-YLOXY)-2-HYDR OXYCYCLOHEXYLOXY)-5-METHYL-4-(METHYLAMINO)-TETRAHYDRO-2H-PYRAN-3,5-DIOL ; × 4 MAGNESIUM ION × 58 water × 14	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS17_ECOLI
Isoform	—
PDB entities	10
Chains and sequence ranges	Author chain Q； PDBConstruct 1–84； UniProt 1–84

Small ribosomal subunit protein bS18

OrganismNot specified

UniProt P0A7T7

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 12 RNA 2	16S rRNA × 1 Small ribosomal subunit protein uS4 × 1 （P0A7V8） Small ribosomal subunit protein uS5 × 1 （P0A7W1） Small ribosomal subunit protein bS6, non-modified isoform × 1 （P02358） Small ribosomal subunit protein uS8 × 1 （P0A7W7） Small ribosomal subunit protein uS11 × 1 （P0A7R9） Small ribosomal subunit protein uS12 × 1 （P0A7S3） Small ribosomal subunit protein uS15 × 1 （P0ADZ4） 30S ribosomal protein S16 × 1 （P0A7T3） Small ribosomal subunit protein uS17 × 1 （P0AG63） 30S ribosomal protein S20 × 1 （P0A7U7） Small ribosomal subunit protein bS21 × 1 （P68679） 23S rRNA × 1 POTASSIUM ION × 24 ;(2R,3R,4R,5R)-2-((1S,2S,3R,4S,6R)-4,6-DIAMINO-3-((2R,3R,6S)-3-AMINO-6-(AMINOMETHYL)-TETRAHYDRO-2H-PYRAN-2-YLOXY)-2-HYDR OXYCYCLOHEXYLOXY)-5-METHYL-4-(METHYLAMINO)-TETRAHYDRO-2H-PYRAN-3,5-DIOL ; × 4 MAGNESIUM ION × 58 water × 14	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS18_ECOLI
Isoform	—
PDB entities	11
Chains and sequence ranges	Author chain R； PDBConstruct 1–75； UniProt 1–75

30S ribosomal protein S20

OrganismNot specified

UniProt P0A7U7

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 12 RNA 2	16S rRNA × 1 Small ribosomal subunit protein uS4 × 1 （P0A7V8） Small ribosomal subunit protein uS5 × 1 （P0A7W1） Small ribosomal subunit protein bS6, non-modified isoform × 1 （P02358） Small ribosomal subunit protein uS8 × 1 （P0A7W7） Small ribosomal subunit protein uS11 × 1 （P0A7R9） Small ribosomal subunit protein uS12 × 1 （P0A7S3） Small ribosomal subunit protein uS15 × 1 （P0ADZ4） 30S ribosomal protein S16 × 1 （P0A7T3） Small ribosomal subunit protein uS17 × 1 （P0AG63） Small ribosomal subunit protein bS18 × 1 （P0A7T7） Small ribosomal subunit protein bS21 × 1 （P68679） 23S rRNA × 1 POTASSIUM ION × 24 ;(2R,3R,4R,5R)-2-((1S,2S,3R,4S,6R)-4,6-DIAMINO-3-((2R,3R,6S)-3-AMINO-6-(AMINOMETHYL)-TETRAHYDRO-2H-PYRAN-2-YLOXY)-2-HYDR OXYCYCLOHEXYLOXY)-5-METHYL-4-(METHYLAMINO)-TETRAHYDRO-2H-PYRAN-3,5-DIOL ; × 4 MAGNESIUM ION × 58 water × 14	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS20_ECOLI
Isoform	—
PDB entities	12
Chains and sequence ranges	Author chain T； PDBConstruct 1–87； UniProt 1–87

Small ribosomal subunit protein bS21

OrganismNot specified

UniProt P68679

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 12 RNA 2	16S rRNA × 1 Small ribosomal subunit protein uS4 × 1 （P0A7V8） Small ribosomal subunit protein uS5 × 1 （P0A7W1） Small ribosomal subunit protein bS6, non-modified isoform × 1 （P02358） Small ribosomal subunit protein uS8 × 1 （P0A7W7） Small ribosomal subunit protein uS11 × 1 （P0A7R9） Small ribosomal subunit protein uS12 × 1 （P0A7S3） Small ribosomal subunit protein uS15 × 1 （P0ADZ4） 30S ribosomal protein S16 × 1 （P0A7T3） Small ribosomal subunit protein uS17 × 1 （P0AG63） Small ribosomal subunit protein bS18 × 1 （P0A7T7） 30S ribosomal protein S20 × 1 （P0A7U7） 23S rRNA × 1 POTASSIUM ION × 24 ;(2R,3R,4R,5R)-2-((1S,2S,3R,4S,6R)-4,6-DIAMINO-3-((2R,3R,6S)-3-AMINO-6-(AMINOMETHYL)-TETRAHYDRO-2H-PYRAN-2-YLOXY)-2-HYDR OXYCYCLOHEXYLOXY)-5-METHYL-4-(METHYLAMINO)-TETRAHYDRO-2H-PYRAN-3,5-DIOL ; × 4 MAGNESIUM ION × 58 water × 14	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS21_ECOLI
Isoform	—
PDB entities	13
Chains and sequence ranges	Author chain U； PDBConstruct 1–71； UniProt 1–71

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	8cgu
Deposition date deposition_date	2023-02-06
Structure title title	Gentamicin bound to the 30S body
Keywords keywords	Antibiotic, RIBOSOME; RIBOSOME
Experimental Method method	ELECTRON MICROSCOPY

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

8cgu__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

8cgu__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

8cgu__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	54.46 Å
Rg (electron density)	54.92 Å
Total Rg	54.94 Å
Atom count	33325
Residues	2332
Excluded volume	509640 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`8cgu__assembly_1__model_1`	14-meric （14）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (18)

Entity 1 Entity 1 polymer

Description description	16S rRNA
Molecular weight formula_weight	499197.9 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polyribonucleotide
Chain IDs strand_id	A
Atom count atom_count	35196

AAAUUGAAGAGUUUGAUCAUGGCUCAGAUUGAACGCUGGCGGCAGGCCUAACACAUGCAAGUCGAACGGUAACAGGAAGAAGCUUGCUUCUUUGCUGACGAGUGGCGGACGGGUGAGUAAUGUCUGGGAAACUGCCUGAUGGAGGGGGAUAACUACUGGAAACGGUAGCUAAUACCGCAUAACGUCGCAAGACCAAAGAGGGGGACCUUCGGGCCUCUUGCCAUCGGAUGUGCCCAGAUGGGAUUAGCUAGUAGGUGGGGUAACGGCUCACCUAGGCGACGAUCCCUAGCUGGUCUGAGAGGAUGACCAGCCACACUGGAACUGAGACACGGUCCAGACUCCUACGGGAGGCAGCAGUGGGGAAUAUUGCACAAUGGGCGCAAGCCUGAUGCAGCCAUGCCGCGUGUAUGAAGAAGCCCUUCGGGUUGUAAAGUACUUUCAGCGGGGAGGAAGGGAGUAAAGUUAAUACCUUUGCUCAUUGACGUUACCCGCAGAAGAAGCACCGGCUAACUCCGUGCCAGCAGCCGCGGUAAUACGGAGGGUGCAAGCGUUAAUCGGAAUUACUGGGCGUAAAGCGCACGCAGGCGGUUUGUUAAGUCAGAUGUGAAAUCCCCGGGCUCAACCUGGGAACUGCAUCUGAUACUGGCAAGCUUGAGUCUCGUAGAGGGGGGUAGAAUUCCAGGUGUAGCGGUGAAAUGCGUAGAGAUCUGGAGGAAUACCGGUGGCGAAGGCGGCCCCCUGGACGAAGACUGACGCUCAGGUGCGAAAGCGUGGGGAGCAAACAGGAUUAGAUACCCUGGUAGUCCACGCCGUAAACGAUGUCGACUUGGAGGUUGUGCCCUUGAGGCGUGGCUUCCGGAGCUAACGCGUUAAGUCGACCGCCUGGGGAGUACGGCCGCAAGGUUAAAACUCAAAUGAAUUGACGGGGGCCCGCACAAGCGGUGGAGCAUGUGGUUUAAUUCGAUGCAACGCGAAGAACCUUACCUGGUCUUGACAUCCACGGAAGUUUUCAGAGAUGAGAAUGUGCCUUCGGGAACCGUGAGACAGGUGCUGCAUGGCUGUCGUCAGCUCGUGUUGUGAAAUGUUGGGUUAAGUCCCGCAACGAGCGCAACCCUUAUCCUUUGUUGCCAGCGGUCCGGCCGGGAACUCAAAGGAGACUGCCAGUGAUAAACUGGAGGAAGGUGGGGAUGACGUCAAGUCAUCAUGGCCCUUACGACCAGGGCUACACACGUGCUACAAUGGCGCAUACAAAGAGAAGCGACCUCGCGAGAGCAAGCGGACCUCAUAAAGUGCGUCGUAGUCCGGAUUGGAGUCUGCAACUCGACUCCAUGAAGUCGGAAUCGCUAGUAAUCGUGGAUCAGAAUGCCACGGUGAAUACGUUCCCGGGCCUUGUACACACCGCCCGUCACACCAUGGGAGUGGGUUGCAAAAGAAGUAGGUAGCUUAACCUUCGGGAGGGCGCUUACCACUUUGUGAUUCAUGACUGGGGUGAAGUCGUAACAAGGUAACCGUAGGGGAACCUGCGGUUGGAUCACCUCCU

Entity 2 Entity 2 polymer

Description description	Small ribosomal subunit protein uS4
Molecular weight formula_weight	23514.2 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	D

MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGARKPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVVYRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALELAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK

Entity 3 Entity 3 polymer

Description description	Small ribosomal subunit protein uS5
Molecular weight formula_weight	17629.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	E

MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNGRVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEGTGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKSVEEILGK

Entity 4 Entity 4 polymer

Description description	Small ribosomal subunit protein bS6, non-modified isoform
Molecular weight formula_weight	15211.1 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	F

MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGRRQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPMVKAKDERRERRDDFANETADDAEAGDSEE

Entity 5 Entity 5 polymer

Description description	Small ribosomal subunit protein uS8
Molecular weight formula_weight	14146.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	H

MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEGFIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIAVVSTSKGVMTDRAARQAGLGGEIICYVA

Entity 6 Entity 6 polymer

Description description	Small ribosomal subunit protein uS11
Molecular weight formula_weight	13872.0 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	K

MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGWATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNAAGFRITNITDVTPIPHDGCRPPKKRRV

Entity 7 Entity 7 polymer

Description description	Small ribosomal subunit protein uS12
Molecular weight formula_weight	13814.2 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	L

MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKKPNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKXLPGVRYHTVRGALDCSGVKDRKQARSKYGVKRPKA

Entity 8 Entity 8 polymer

Description description	Small ribosomal subunit protein uS15
Molecular weight formula_weight	10290.8 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	O

MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFAEHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR

Entity 9 Entity 9 polymer

Description description	30S ribosomal protein S16
Molecular weight formula_weight	9207.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	P

MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIASEKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA

Entity 10 Entity 10 polymer

Description description	Small ribosomal subunit protein uS17
Molecular weight formula_weight	9724.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Q

MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKLHVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL

Entity 11 Entity 11 polymer

Description description	Small ribosomal subunit protein bS18
Molecular weight formula_weight	9005.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	R

MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRITGTRAKYQRQLARAIKRARYLSLLPYTDRHQ

Entity 12 Entity 12 polymer

Description description	30S ribosomal protein S20
Molecular weight formula_weight	9708.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	T

MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDKAAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA

Entity 13 Entity 13 polymer

Description description	Small ribosomal subunit protein bS21
Molecular weight formula_weight	8524.0 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	U

MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTERKRAKASAVKRHAKKLARENARRTRLY

Entity 14 Entity 14 polymer

Description description	23S rRNA
Molecular weight formula_weight	941811.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polyribonucleotide
Chain IDs strand_id	a

GGUUAAGCGACUAAGCGUACACGGUGGAUGCCCUGGCAGUCAGAGGCGAUGAAGGACGUGCUAAUCUGCGAUAAGCGUCGGUAAGGUGAUAUGAACCGUUAUAACCGGCGAUUUCCGAAUGGGGAAACCCAGUGUGUUUCGACACACUAUCAUUAACUGAAUCCAUAGGUUAAUGAGGCGAACCGGGGGAACUGAAACAUCUAAGUACCCCGAGGAAAAGAAAUCAACCGAGAUUCCCCCAGUAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCUGAAUCAGUGUGUGUGUUAGUGGAAGCGUCUGGAAAGGCGCGCGAUACAGGGUGACAGCCCCGUACACAAAAAUGCACAUGCUGUGAGCUCGAUGAGUAGGGCGGGACACGUGGUAUCCUGUCUGAAUAUGGGGGGACCAUCCUCCAAGGCUAAAUACUCCUGACUGACCGAUAGUGAACCAGUACCGUGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGUGAAAAAGAACCUGAAACCGUGUACGUACAAGCAGUGGGAGCACGCUUAGGCGUGUGACUGCGUACCUUUUGUAUAAUGGGUCAGCGACUUAUAUUCUGUAGCAAGGUUAACCGAAUAGGGGAGCCGAAGGGAAACCGAGUCUUAACUGGGCGUUAAGUUGCAGGGUAUAGACCCGAAACCCGGUGAUCUAGCCAUGGGCAGGUUGAAGGUUGGGUAACACUAACUGGAGGACCGAACCGACUAAUGUUGAAAAAUUAGCGGAUGACUUGUGGCUGGGGGUGAAAGGCCAAUCAAACCGGGAGAUAGCUGGUUCUCCCCGAAAGCUAUUUAGGUAGCGCCUCGUGAAUUCAUCUCCGGGGGUAGAGCACUGUUUCGGCAAGGGGGUCAUCCCGACUUACCAACCCGAUGCAAACUGCGAAUACCGGAGAAUGUUAUCACGGGAGACACACGGCGGGUGCUAACGUCCGUCGUGAAGAGGGAAACAACCCAGACCGCCAGCUAAGGUCCCAAAGUCAUGGUUAAGUGGGAAACGAUGUGGGAAGGCCCAGACAGCCAGGAUGUUGGCUUAGAAGCAGCCAUCAUUUAAAGAAAGCGUAAUAGCUCACUGGUCGAGUCGGCCUGCGCGGAAGAUGUAACGGGGCUAAACCAUGCACCGAAGCUGCGGCAGCGACGCUUAUGCGUUGUUGGGUAGGGGAGCGUUCUGUAAGCCUGCGAAGGUGUGCUGUGAGGCAUGCUGGAGGUAUCAGAAGUGCGAAUGCUGACAUAAGUAACGAUAAAGCGGGUGAAAAGCCCGCUCGCCGGAAGACCAAGGGUUCCUGUCCAACGUUAAUCGGGGCAGGGUGAGUCGACCCCUAAGGCGAGGCCGAAAGGCGUAGUCGAUGGGAAACAGGUUAAUAUUCCUGUACUUGGUGUUACUGCGAAGGGGGGACGGAGAAGGCUAUGUUGGCCGGGCGACGGUUGUCCCGGUUUAAGCGUGUAGGCUGGUUUUCCAGGCAAAUCCGGAAAAUCAAGGCUGAGGCGUGAUGACGAGGCACUACGGUGCUGAAGCAACAAAUGCCCUGCUUCCAGGAAAAGCCUCUAAGCAUCAGGUAACAUCAAAUCGUACCCCAAACCGACACAGGUGGUCAGGUAGAGAAUACCAAGGCGCUUGAGAGAACUCGGGUGAAGGAACUAGGCAAAAUGGUGCCGUAACUUCGGGAGAAGGCACGCUGAUAUGUAGGUGAGGUCCCUCGCGGAUGGAGCUGAAAUCAGUCGAAGAUACCAGCUGGCUGCAACUGUUUAUUAAAAACACAGCACUGUGCAAACACGAAAGUGGACGUAUACGGUGUGACGCCUGCCCGGUGCCGGAAGGUUAAUUGAUGGGGUUAGCGCAAGCGAAGCUCUUGAUCGAAGCCCCGGUAAACGGCGGCCGUAACXAUAACGGUCCUAAGGUAGCGAAAUUCCUUGUCGGGUAAGUUCCGACCUGCACGAAUGGCGUAAUGAUGGCCAGGCUGUCUCCACCCGAGACUCAGUGAAAUUGAACUCGCUGUGAAGAUGCAGUGUACCCGCGGCAAGACGGAAAGACCCCGUGAACCUUUACUAUAGCUUGACACUGAACAUUGAGCCUUGAUGUGUAGGAUAGGUGGGAGGCUUUGAAGUGUGGACGCCAGUCUGCAUGGAGCCGACCUUGAAAUACCACCCUUUAAUGUUUGAUGUUCUAACGUUGACCCGUAAUCCGGGUUGCGGACAGUGUCUGGUGGGUAGUUUGACUGGGGCGGUCUCCUCCUAAAGAGUAACGGAGGAGCACGAAGGUUGGCUAAUCCUGGUCGGACAUCAGGAGGUUAGUGCAAUGGCAUAAGCCAGCUUGACUGCGAGCGUGACGGCGCGAGCAGGUGCGAAAGCAGGUCAUAGUGAUCCGGUGGUUCUGAAUGGAAGGGCCAUCGCUCAACGGAUAAAAGGUACUCCGGGGAUAACAGGCUGAUACCGCCCAAGAGUUCAUAUCGACGGCGGUGUUUGGCACCUCGAUGUCGGCUCAUCACAUCCUGGGGCUGAAGUAGGUCCCAAGGGUAUGGCUGUUCGCCAUUUAAAGUGGUACGCGAGCUGGGUUUAGAACGUCGUGAGACAGUUCGGUCCCUAUCUGCCGUGGGCGCUGGAGAACUGAGGGGGGCUGCUCCUAGUACGAGAGGACCGGAGUGGACGCAUCACUGGUGUUCGGGUUGUCAUGCCAAUGGCACUGCCCGGUAGCUAAAUGCGGAAGAGAUAAGUGCUGAAAGCAUCUAAGCACGAAACUUGCCCCGAGAUGAGUUCUCCCUGACCCUUUAAGGGUCCUGAAGGAACGUUGAAGACGACGACGUUGAUAGGCCGGGUGUGUAAGCGCAGCGAUGCGUUGAGCUAACCGGUACUAAUGAACCGUGAGGCUUAACCUU

Entity 15 Entity 15 non-polymer

Description description	POTASSIUM ION
Molecular weight formula_weight	39.1 kDa
Source method src_method	syn
Copy count entity_count	24

Entity 16 Entity 16 non-polymer

Description description	;(2R,3R,4R,5R)-2-((1S,2S,3R,4S,6R)-4,6-DIAMINO-3-((2R,3R,6S)-3-AMINO-6-(AMINOMETHYL)-TETRAHYDRO-2H-PYRAN-2-YLOXY)-2-HYDR OXYCYCLOHEXYLOXY)-5-METHYL-4-(METHYLAMINO)-TETRAHYDRO-2H-PYRAN-3,5-DIOL ;
Molecular weight formula_weight	449.5 kDa
Source method src_method	syn
Copy count entity_count	4

Entity 17 Entity 17 non-polymer

Description description	MAGNESIUM ION
Molecular weight formula_weight	24.3 kDa
Source method src_method	syn
Copy count entity_count	58

Entity 18 Entity 18 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	1871

▼

6. Fold Classification (SCOP + CATH) 6 domains

CATH v4.4 (6 domains)

Domain ID domain_id	8cguD01
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	1050 — Ribosomal Protein S4 Delta 41; Chain A, domain 1
Homologous superfamily homologous superfamily	10 — Ribosomal protein S4/S9, N-terminal domain

Domain ID domain_id	8cguD02
Class class	3 — Alpha Beta
Architecture architecture	10 — Roll
Topology topology	290 — Structural Genomics Hypothetical 15.5 Kd Protein In mrcA-pckA Intergenic Region; Chain A
Homologous superfamily homologous superfamily	10 — RNA-binding S4 domain

Domain ID domain_id	8cguE01
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	160 — Double Stranded RNA Binding Domain
Homologous superfamily homologous superfamily	20 —

Domain ID domain_id	8cguE02
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	230 — Ribosomal Protein S5; domain 2
Homologous superfamily homologous superfamily	10 —

Domain ID domain_id	8cguH01
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	1370 — Ribosomal Protein S8; Chain: A, domain 1
Homologous superfamily homologous superfamily	30 —

Domain ID domain_id	8cguH02
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	1490 — Dna Ligase; domain 1
Homologous superfamily homologous superfamily	10 —

▶

Unit-cell length a length_a	1.000 Å
Unit-cell length b length_b	1.000 Å
Unit-cell length c length_c	1.000 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	—

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	—

Resolution resolution	1.890 Å
Low-resolution limit resolution_low	207.420 Å
R factor r_factor	0.2605
R_work r_work	0.2605
R_free r_free	—

Resolution resolution	1.89 Å
Resolution测定Method resolution_method	—
颗粒数 num_particles	1301160

Gentamicin bound to the 30S body

1. Protein Identity and Related Structures Protein Identity & Related Structures

Small ribosomal subunit protein uS4

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Small ribosomal subunit protein uS5

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Small ribosomal subunit protein bS6, non-modified isoform

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Small ribosomal subunit protein uS8

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Small ribosomal subunit protein uS11

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Small ribosomal subunit protein uS12

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Small ribosomal subunit protein uS15

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

30S ribosomal protein S16

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Small ribosomal subunit protein uS17

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Small ribosomal subunit protein bS18

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer