8i9p

Cryo-EM structure of a Chaetomium thermophilum pre-60S ribosomal subunit - State Mak16

Method: ELECTRON MICROSCOPY D_max: 223.6 Å Quality: EXCELLENT

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	8i9p
沉积日期 deposition_date	2023-02-07
结构标题 title	Cryo-EM structure of a Chaetomium thermophilum pre-60S ribosomal subunit - State Mak16
关键词 keywords	Ribosome, Ribosome biogenesis, pre-60S ribosome; RIBOSOME
实验方法 method	ELECTRON MICROSCOPY

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	78.43 Å
回转半径 Rg (电子) rg_electron	81.08 Å
零角强度 I(0) i0	33413600000.00
分子量 molecular_weight	1178900.0 kDa
排除体积 excluded_volume	1321400 Å³
包络体积 envelope_volume	2219700 Å³
水化壳体积 shell_volume	225990 Å³
包络直径 envelope_diameter	305.7 Å
壳层 Rg shell_rg	78.71 Å
包络 Rg envelope_rg	81.58 Å
形状 Rg shape_rg	81.19 Å
总 Rg total_rg	80.79 Å
总原子数 total_atoms	80799
残基数 n_residues	7432
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	223.6 Å
Rg (实空间) rg_real	74.15 Å
Rg 误差 (实空间) rg_real_error	0.77 Å
I(0) (实空间) i0_real	3.1990e+10
I(0) 误差 (实空间) i0_real_error	6.1050e+08
Rg (倒空间) rg_reciprocal	76.59 Å
I(0) (倒空间) i0_reciprocal	33250000000.0000
解质量估计 total_estimate	0.9078
解质量评级 solution_quality	EXCELLENT a EXCELLENT solution
P(r) 峰数 n_peaks	1
主峰位置 r_peak_primary	78.3 Å
偏度 Skewness skewness	0.442
峰度 Kurtosis kurtosis	-0.302
角度范围 angular_range	— – 0.1000 Å⁻¹
当前正则化参数 α current_alpha	0.6817
最高正则化参数 α highest_alpha	4255000000.0000
实空间数据点数 n_real_points	21
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.001; Oscil: 0.947; Stabil: 0.980; Sysdev: 1.000; Positv: 1.000; Valcen: 0.969; Smooth: 0.052

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (33)

Entity 1 polymer

描述 description	RNA (3341-MER)
分子量 formula_weight	1079977.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polyribonucleotide
链编号 strand_id	C1
原子数 atom_count	80799

GGUUGACCUCGGAUCAGGUAGGAGGACCCGCUGAACUUAAGCAUAUCAAUAAGCGGAGGAAAAGAAACCAACAGGGAUUGCCCUAGUAACGGCGAGUGAAGCGGCAACAGCUCAAAUUUGAAAGCUGGCUUCGGCCCGCGUUGUAAUUUGGAGAGGAUGCUUUGGGCGAGGCUCCUUCUGAGUUCCCUGGAACGGGACGCCACAGAGGGUGAGAGCCCCGUAUAGUUGGAAGCCAAGCCUGUGUAAAGCUCCUUCGACGAGUCGAGUAGUUUGGGAAUGCUGCUCAAAAUGGGAGGUAAAUUUCUUCUAAAGCUAAAUACCGGCCAGAGACCGAUAGCGCACAAGUAGAGUGAUCGAAAGAUGAAAAGCACUUUGAAAAGAGGGUUAAAUAGCACGUGAAAUUGUUGAAAGGGAAGCGCUUGUGACCAGACUUGCGCCCGGCGGAUCAUCCGGUGUUCUCACCGGUGCACUCCGCCGGGCUCAGGCCAGCAUCGGUUCUGGCGGGGGGAUAAAGGCCCAGGGAAUGUGGCUCCUCCGGGAGUGUUAUAGCCCUGGGUGUAAUACCCUCGCCGGGACCGAGGACCGCGCUCUGCAAGGAUGCUGGCGUAAUGGUCACCAGCGACCCGUCUUGAAACACGGACCAAGGAGUCAAGGUUUUGCGCGAGUGUUUGGGUGUAAAACCCGCACGCGUAAUGAAAGUGAACGUAGGUGAGAGCUUCGGCGCAUCAUCGACCGAUCCUGAUGUAUUCGGAUGGAUUUGAGUAGGAGCGUUAAGCCUUGGACCCGAAAGAUGGUGAACUAUGCUUGGAUAGGGUGAAGCCAGAGGAAACUCUGGUGGAGGCUCGCAGCGGUUCUGACGUGCAAAUCGAUCGUCAAAUCUGAGCAUGGGGGCGAAAGACUAAUCGAACCAUCUAGUAGCUGGUUACCGCCGAAGUUUCCCUCAGGAUAGCAGUGUCGACCUUCAGUUUUAUGAGGUAAAGCGAAUGAUUAGGGACUCGGGGGCGAUUUUUAGCCUUCAUCCAUUCUCAAACUUUAAAUAUGUAAGAAGCCCUUGUUACUUAACUGAACGUGGGCAUUCGAAUGUAUCGACACUAGUGGGCCAUUUUUGGUAAGCAGAACUGGCGAUGCGGGAUGAACCGAACGCGGGGUUAAGGUGCCGGAGUGGACGCUCAUCAGACACCACAAAAGGCGUUAGUACAUCUUGACAGCAGGACGGUGGCCAUGGAAGUCGGAAUCCGCUAAGGACUGUGUAACAACUCACCUGCCGAAUGUACUAGCCCUGAAAAUGGAUGGCGCUCAAGCGUCCCACCCAUACCCCGCCCUCAGGGUAGAAACGAUGCCCUGAGGAGUAGGCGGCCGUGGAGGUCAGUGACGAAGCCUAGGGCGUGAGCCCGGGUCGAACGGCCUCUAGUGCAGAUCUUGGUGGUAGUAGCAAAUACUUCAAUGAGAACUUGAAGGACCGAAGUGGGGAAAGGUUCCAUGUGAACAGCGGUUGGACAUGGGUUAGUCGAUCCUAAGCCAUAGGGAAGUUCCGUUUCAAAGGGGCACUCGUGCCCCGUGUGGCGAAAGGGAAGCCGGUUAAUAUUCCGGCACCUGGAUGUGGGUUUUGCGCGGCAACGCAACUGAACGCGGAGACGACGGCGGGGGCCCCGGGCAGAGUUCUCUUUUCUUCUUAACGGUCUAUCACCCUGGAAACAGUUUGUCUGGAGAUAGGGUUUAAUGGCCGGAAGAGCCCGACACUUCUGUCGGGUCCGGUGCGCUCUCGACGUCCCUUGAAAAUCCGCGGGAGGGAAUAAUUCUCACGCCAGGUCGUACUCAUAACCGCAGCAGGUCCCCAAGGUGAACAGCCUCUGGUUGAUAGAACAAUGUAGAUAAGGGAAGUCGGCAAAAUAGAUCCGUAACUUCGGGAAAAGGAUUGGCUCUAAGGGUUGGGCACGUUGGGCUUUGGGCGGACGCCCUGGGAGCAGAGGGCCUCUAGCCGGGCAACCGGCCGGCGGCCCUCAGCACCCGGGGUUGAAGCCCUUAGCAGGCUUCGGCCGUCCGGCGUGCGGUUAACAACCAACUUAGAACUGGUACGGACAGGGGGAAUCUGACUGUCUAAUUAAAACAUAGCAUUGCGAUGGCCAGAAAGUGGUGUUGACGCAAUGUGAUUUCUGCCCAGUGCUCUGAAUGUCAAAGUGAAGAAAUUCAACCAAGCGCGGGUAAACGGCGGGAGUAACUAUGACUCUCUUAAGGUAGCCAAAUGCCUCGUCAUCUAAUUAGUGACGCGCAUGAAUGGAUUAACGAGAUUCCCACUGUCCCUAUCUACUAUCUAGCGAAACCACAGCCAAGGGAACGGGCUUGGCAAAAUCAGCGGGGAAAGAAGACCCUGUUGAGCUUGACUCUAGUUUGACAUUGUGAAAAGACAUAGGAGGUGUAGAAUAGGUGGGAGCUUCGGCGCCAGUGAAAUACCACUACUCCUAUUGUUUUUUUACUUAUUCAAUGAAGCGGGGCUGGACUUGCGUCCAACUUCUGGAGUUAAGGUCCUUCGCGGGCCGACCCGGGUUGAAGACAUUGUCAGGUGGGGAGUUUGGCUGGGGCGGCACAUCUGUUAAACCAUAACGCAGGUGUCCUAAGGGGGGCUCAUGGAGAACAGAAAUCUCCAGUAGAACAAAAGGGUAAAAGUCCCCUUGAUUUUGAUUUUCAGUGUGAAUACAAACCAUGAAAGUGUGGCCUAUCGAUCCUUUAGUCCCUCGAAAUUUGAGGCUAGAGGUGCCAGAAAAGUUACCACAGGGAUAACUGGCUUGUGGCGGCCAAGCGUUCAUAGCGACGUCGCUUUUUGAUCCUUCGAUGUCGGCUCUUCCUAUCAUACCGAAGCAGAAUUCGGUAAGCGUUGGAUUGUUCACCCACUAAUAGGGAACGUGAGCUGGGUUUAGACCGUCGUGAGACAGGUUAGUUUUACCCUACUGAUGAACUCGUCGCAAUGGUAAUUCAGCUUAGUACGAGAGGAACCGCUGAUUCAGAUAAUUGGUUUUUGCGGUUGUCCGACCGGGCAGUGCCGCGAAGCUACCAUCUGCUGGAUAAUGGCUGAACGCCUCUAAGUCAGAAUCCAUGCCAGAACGCGACGAUACUACCCGCACGUUGUAGACGUAUAAGAAUAGGCUCCGGCCUCGUAUCCUAGCAGGCGAUUCCUCCGCCGGCCUCGAAGUGGCCGUCGGUAAUUCGCGUAUUGCAAUUUAGACACGCGCGGGAUCAAAUCCUUUGCAGACGACUUAGAUGUGCGAAAGGGUCCUGUAAGCAGUAGAGUAGCCUUGUUGUUACGAUCUGCUGAGGGUAAGCCCUCCUUCGCCUAGAUUUCCCAG

Entity 2 polymer

描述 description	RNA (306-MER)
分子量 formula_weight	98523.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polyribonucleotide
链编号 strand_id	C2

AAACUUUCAACAACGGAUCUCUUGGUUCUGGCAUCGAUGAAGAACGCAGCGAAAUGCGAUAAGUAAUGUGAAUUGCAGAAUUCCGUGAAUCAUCGAAUCUUUGAACGCACAUUGCGCCCGCCGGUAUUCCGGCGGGCAUGCCUGUUCGAGCGUCAUUUCAACCAUCAAGCCCUGGGCUUGUGUUGGGGACCCGCGGCUGCUCGCGGGCCCUGAAAAGCAGUGGCGGGCUCGCUAGUCACACCGAGCGUAGUAGAACUUCUAUUCGAUCUCGCUCAGGGCGUGCGGCGGGUGCCAGCCGUAAAACCC

Entity 3 polymer

描述 description	Brix domain-containing protein
分子量 formula_weight	36482.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	CA

MAAVYKTIAKVTGSKEDKDKEDRSNIIAERKNKQRVLVLSSRGVTYRHRHLLNDLASMLPHGRKDAKFDTKSRLYELCELAELYNCNNVLFFEARKGKDLYMWFSKVPNGPTVKFYAQNLHTMEELHFQGNCLKGSRPILSFDAAFEQEPYLKVIKELFLHTFGVPQGHKKSKPFIDHVLSFSVADGKIWVRNYEIREVEKVKGEADKREEGETEKPKAAKPGSKDTDINLIEIGPRFVLTPIIIQEGSFGGPILYENKRFISPNKIRAELRKAKAARHHARMEQQRDLLARKRQLGLDESTPKKKDDLDTRELFA

Entity 4 polymer

描述 description	Ribosome biogenesis protein C8F11.04
分子量 formula_weight	43700.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	CB

MAPSTAVAKKETDAVIPVDPDQTLKACKALLAHIKKAAAAPRPDGKQNLLADEESTVAETPIWLTLTTKKHIHDSHRLQPGKIILPHPLNTSEEISVCLITADPQRFYKNAVADEFPEDLRAKIGRVIDISHLKAKFKAYEAQRKLFSEHDVFLADTRIINRLPKALGKTFYKTTTKRPIPVVLMAQREKVNGKRVPAPKGKKEKRDPLENANARPIPEIVAEIRKAIGAALVHLSPSTNTAIKVGYANWEPEKLAANIETVIRELVERFVPQKWQNVRNFYVKGPETAALPIYQTDELWLDESKVVPDGQEPARALPGKREKANIGKKRKPLEDASQPALEETGKDERPKKKAKKTLPESNDDKLDKAIAERKEQLKKQKAAAKKVAADI

Entity 5 polymer

描述 description	Ribosome biogenesis protein ERB1
分子量 formula_weight	90838.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	CC

MGSKIVEKKRKSRDSDSESDNELGDGLFDGVLSQSEDEEDYIPSSEVDEDDDDDADESASEDSDDSNDSEDDEVEEDDEALLSDEIPSEGESEKDQDLAESKESKQDQDKEPSEPEILEPFVDPPRKEDEELEDRNYRIEKDANGGIRYVYDEIDPVYDSDDTDYNVPVNTIGNIPLSFYDSYPHIGYDINGKKIMRPATGDALQNLLDSIEVPEGWTGLTDPNTGKPLNLSRDELELIRKVQQGLIPDDVEDPYPDTVEWFTSVEEKMPLSAAPEPKRRFIPSKNEAKQIMKLVRAIREGRILPYKPPEEREREELEKEEEFYDLWQNEEPQPPNPMHIPAPKLPPPGYDLSYNPPPEYLPTKEEREEWEKMDPEDREKDYLPTKYDSLRKVPAWGNFVKERFERCMDLYLAPRVRKNRLNIDPNSLLPKLPSPDELKPFPTVQQTIFRGHEGRVRSVAIDPTGVALATGGDDGTVRVWELLTGRQVWSVKLNGDEAVNTVRWRPTKDTFILAAAAGEDIFLMIPTHPSVTPALDQASRDILNAGFGHATNGKQQANLPPGKEPPGKWARPGTRLEDEGVLLRITVRSTIKAISWHRRGDHFATVSPSGQRSSVAIHTLSKHLTQIPFRKLNGLAQTASFHPLRPLFFVATQRSIRCYDLQKLELVKIVQPGAKWISSFDVHPGGDNLVVGSYDKRLLWHDLDLSNRPYKTMRFHTEAIRAVRFHKGGLPLFADASDDGSLQIFHGKVPNDQLENPTIVPVKMLKGHKVVNKLGVLDIDWHPREPWCVSAGADGTARLWM

Entity 6 polymer

描述 description	RNA helicase
分子量 formula_weight	66830.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	CE

MASESSKKRKFKDSKGPKPDEAVAKPSKFKKLKRDPTPEERETSDSESGNSDGQHEPDQVDSDGEDAVSDDGSFDEKVAHQDEDGSRPENGTDLEDVPKNDNLLPPPTNAQEFSELNLSEKTTKAIAEMGFTKMTEIQRRAIPPALAGKDVLGAAKTGSGKTLAFLIPAVEMLSSLRFKPRNGTGAIVVTPTRELALQIFGVARELMKYHSQTYGVVIGGANRRAEAEKLGKGVNLLIATPGRLLDHLQNTPFVFKNLKSLIIDEADRILEIGFEDEMRQIVKILPKEDRQTMLFSATQTTKVEDLARISLRPGPLYINVDEEKKYSTVEGLEQGYVVVEADKRFLLLFSFLKKMAKKKIIVFFSSCNSVKYYSELLQYIDLPVLDLHGKQKQQKRTNTFFEFCNAKSGTLICTDVAARGLDIPQVDWIVQFDPPDDPRDYIHRVGRTARGNNGKGRSLLFLQPCELGFLAHLKAAKVPVVEYDFPKNKILNVQSQLEKLISTNYYLNQSAKEGYRSYIHAYASHSLRSVFDVHKLDLVKVAKSFGFSTPPRVDITLSASLSRDKKPQGRRAYGSQPRQGGRYKKVGDIPDPQPNTFG

Entity 7 polymer

描述 description	Putative RNA-binding protein
分子量 formula_weight	45884.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	CI

MAAELRKKKSKSAVADADAAISKKEKATKVTKATPKAEKRKAPEEASPVAIKKQKADKDAVVKKAAVAAEDKIAEKPVKSVLKKETSAKAKEAAKPKKKQEKVEEEEEEALENADAAFGSDEESELDEQTKALMDTLDDGEESDEDESKQVSTFKKGQDVGKIPTPKKAPQKQTNGDSKPAGDSQPGVMYIARLPHGFYEHELRGYFSQFGEITRLRVVRNKKTGASRHRAFIEFADAEVADIAARTMDKYLLFGHILTCKIVPPAQVHPDLFKGANRRFKVVPWNKMAGRQLERPLSESQWQVKVAKEEQRRAARAEKLKEMGYEFEAPALKVPKAKPVLENGEEEKKAIETAPAPAVEEKESKEVAKRQAEEEVEEKAPAPKTKKVKEDKPAKEAATISAPKKGKKSKKTKS

Entity 8 polymer

描述 description	Pescadillo homolog
分子量 formula_weight	77167.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	CJ

MGKAKKKGKSGAARNYMTRTQAVKKLQLSLPDFRKLCIWKGIYPREPRDRRKVNKSATASTTFYYTKDIQYLLHEPLLQKFREQKALEKKISRALGRGDVSNAARLERNANLPEKTGKPRYTLNHIIRERYPTFQDALRDLDDCLSMLFLFANLPSTTAVPAKMIARCERLCHEFQHYLIVTHSLRKSFLSIKGIYYQANIQGEDILWLVPYKFNQRIVGDVDFRIMGTFVEFYMTLLGFVNYRLYTSIGLKYPPKFDQVKDDQGAELAAFSLEGLNLASQNGEQKAITNGEEHGPDPKVQAEVDKLVAKLREEEQQANGDKTDEKGEENEGDDKPSDAIDKFEPVAPGGDVLPQPSYSSSDPSQLFANFTFFLSRETPRQPLEFILRAFGCKRIGWDAVLGEGAFTTDESDPRITHQIIDRPVIRAAVSEDGDGEDNQTSQKLAPNGRYPGRIYVQPQWVWDSINDEELKPPELYAPGAQLPPHLSPFVKPTQGQYDPTKPLEEQQTEAEALEAELEDAQAQQEGSDEESGSEVENDMSVASDEEDEQEDDFGGFSDEDEEQSDEGSEEGDEEEDDDEEEEATLERQRELEAELAGKAVSKGKPLDPKVKAKLEAKKALERKKKQEAEELERAKGMLSKKKRKLFEQMQYSNAKKNAEDAKLRAKRRRIEKEMAAKKA

Entity 9 polymer

描述 description	60S ribosomal protein l7-like protein
分子量 formula_weight	28821.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	2

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	CM,LF

MSSTTVPTQNDILVPETLLKKRKSQEKARAERAAALEKRKQANKEKRQVIFKRAEKYVKEYREQEREKIRLARIAKQQGSFHIPAEAKLVFVIRIKGINKIPPKPRKILQLLRLRQINNGVFVKVTKATAEMIKIVEPWVAYGYPNLKSVRELIYKRGYGKVNGQRIPLTDNAIIEENLGKYGIICIEDLIHEIFTVGPNFKQAANFLWPFKLSNPNGGFRPRKFKHFIEGGDLGNREEHINALIRAMN

Entity 10 polymer

描述 description	Nucleolar protein 16
分子量 formula_weight	26960.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	CR

MGRELQKRKRRSSRPTIRMPNRRKKALNPAGNNIIAKSWNKKETLSQNYTRFGLVAKLGKATGGTAPGNKALLKDLNDPLAIKSGDQGLIKVSEVKVERDEQGRIKRVVRDNNPLNDPLNDLDSDSESDAVPQQQQQEQPVLNNHIRQHDLELENVAADVDDESKPEVLRALEREATRPVEKTVRHQSEREREWLQRLVDKHGDDVAAMARDRKLNPYQQTASDIKRRLKKAGLLSA

Entity 11 polymer

描述 description	rRNA-processing protein EBP2
分子量 formula_weight	50255.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	CU

MAKKGATQSPKAGSKPNSQTGDAQSKNQKADRRKSNEPVVEKVAEKEEQREEETEESEEDESEDEQLQIRANGNNESDSEEDSDENSEDEDESDDDSGDEHPTHHIDFEAIDDSDSESELDSDEENDKKQDSNKAQNGMSEDKMDVDKDGEDKSGDEEDEEEEIDVEELEPEDEEDEEQIAAGTRTRQTINNKEGLLTALRRIQLDINPKTTPFSFHQSVVASKRTEDSIPQIEDDLQRELAFMNQALEAARLGRSLLRKEGVPFTRPTDYFAEMVRTDATMEKVKQKLIEEATAKKAAAEARKQRDLKKFGKAVQVQKQLERAKQKREALDKINQLKRKRAEGGSAALGATEADDPFDVAVDNELSRDSKPKKRRADGDHPVPNPKRQKKNAKYGFGGKKRGSKSGDAISSGDLSGFSVKRMKYGGGAGGGKKKANRPGKARRKAAAAKR

Entity 12 polymer

描述 description	60S ribosomal protein L4-like protein
分子量 formula_weight	39439.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	LC

MASRPTVTVFGADGKPTGATEVLPKVFSAPIRPDIVKHVHTGMAKNKRQPYAVSEKAGHQTSAESWGTGRAVARIPRVSGGGTHRAGQGAFGNMCRSGRMFAPTKIWRKWHVKINQGQKRFATASALAASAVAPLLMARGHQVSTVPEVPLVVDSAAVAGDAVAKTAAAYKLLKAIGAGPDVEKVKKSKKLRAGKGKMRGRRHRQRRGPLIVYSPEHDGKELVKGFRNIPGVETCPVDALNLLQLAPGGHLGRFIVWTSAAIKQLDAVYESKKGFFLPANIVSQADLSRLINSTEIQSVLRAPKGEARTKRACVQKKNPLRNKQIMLRLNPYASTFAKEKLGEVKAEEGKPPKVPASFKELLHEA

Entity 13 polymer

描述 description	60S ribosomal protein L6
分子量 formula_weight	22133.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	LE

MSAAPTTKTFGKGTRTVPAPSEKAQKWYPAEDEAQPKKVRKAVRPWTPRKSLQPGTVLILLAGRFRGKRVVLLKCLDQGVLLVTGPFKINGVPLRRVNARYVIATSVKVDLTGVDQAKIDEVAQPKYFTAEKAKEKASEEAFFKQGEKPQKKPVSSTRAADQKAIDKALIANIKKVDMLASYLASSFSLRKGDKPHLMKF

Entity 14 polymer

描述 description	60S ribosomal protein L8
分子量 formula_weight	29744.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	LG

MPPKSGKKVAPAPFPQGKAGAKKAPKNPLLEKRPRNFGIGQDIQPKRNLSRMVKWPEYIRLQRQKKILRMRLKVPPAIAQFQYTLDKNLAAQAFKLLNKYRPETKQEKKERLLREATAIKEGKKKEDVSKKPYTVKYGLNHVVGLIENKKASLVLIANDVDPIELVVFLPALCRKMGIPYAIIKGKARLGTLVHKKTAAVVAITEVRSEDKNELAKLISAVKEGYLEKVEDTRKRWGGGIMGFKAQKREEKRKKSLETAIKV

Entity 15 polymer

描述 description	60S ribosomal protein L13
分子量 formula_weight	24191.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	LL

MAIKHNQQIPNNHFRKHWQRRVRCHFDQPGKKVTRRLARRAKAAALAPRPVDKLRPIVRCPTVKYNRRTRLGRGFTLEELKAAGIPRLLAPTIGIAVDHRRKNLSEESLAANVQRLKDYKARLILFPRKSNKPKKADTPKDQQTAETTTSLRTSFGVEQPLAPGFTEISKSEIPAGIEGGAYRALRKARSDARLVGVREKRAKEKAEAEANKK

Entity 16 polymer

描述 description	60S ribosomal protein L14-like protein
分子量 formula_weight	16111.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	LM

MAEINVEATSWRRVEVGRVLKLENGSLAAIVEIIDHKRVLADGPSSDPKLATPRGIVPLSRALLTPIVIPKLPRGARTGAVKKAWEAYGVDAKWKETNWAKKQLQQERRQSLTDFDRFKVMRLKKQRRFEERKALAKIKAAA

Entity 17 polymer

描述 description	Ribosomal protein L15
分子量 formula_weight	24307.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	LN

MGALKYLEELSKKKQSDVVRFLLRVRCWEYRQLNVIHRASRPSRPDKARRLGYKAKQGYVIYRVRVRRGGRKRPVPKGATYGKPTNQGVNQLKYQRSLRATAEERVGRRCSNLRVLNSYWVNQDSTYKYYEVILVDPNHKAIRRDPRINWICNPVHKHRECRGLTSTGKKSRGLNKGHRFNKTRAGRRKTWKRHNTLSLWRYR

Entity 18 polymer

描述 description	60S ribosomal protein L16-like protein
分子量 formula_weight	22993.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	LO

MSSFESVVVIDGKGHLLGRLASIVAKQLLSGQKIVVVRCEALNISGEFFRAKLKYHAYLRKMTRYNPTRGGPFHFRAPSRIFYKAVRGMIPHKTARGAAALERLKVFEGVPPPYDKKKKMVVPQALRVLRLQPGRKYCTVGRLSHEVGWKYQDVVARLEERRKAKGAAYYERKKLAARQLSEAKKAAAAKVDPKVSEALAAYGY

Entity 19 polymer

描述 description	60S ribosomal protein l17-like protein
分子量 formula_weight	21072.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	LP

MVRYAATEIAPTKSARARGSYLRVSFKNTRETAQAINGWKLQRALTFLQNVIDKKEAVPMRRYAGSTGRTAQGKQWGVSRARWPVKSAQFLIGLLKNAEANADAKGLDTGNLIIKHIQVNQAPKQRRRTYRAHGRINPYMSNPCHIELILTEAEETVQKSEAVVRDVESHLSSRQRGVRIRRALTAA

Entity 20 polymer

描述 description	Ribosomal protein L18-like protein
分子量 formula_weight	24195.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	LQ

MDLVCAFIGPLFAPGARRLNLEEIAAESSSGIDLRWKHVRSSHRKAPKSDNVYLKLLVKLYRFLARRTEAPFNKVVLRRLFMSRINRPPVSLSRIARNLKNGNEKKTVVVVATVTDDNRLLQVPKMEVAALRFTAKARARIEAAGGRTLTLDQLALEKPTGANTLLLRGPKNAREAVKHFGFGPHKHKKPYVRSKGRKFERARGRRRSRGFKV

Entity 21 polymer

描述 description	60S ribosomal protein L20
分子量 formula_weight	20341.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	LS

MGRLQEYQVIGRHLPTEANPNPALYRMRIFAPNEVVAKSRFWYFLRGLKKVKKATGEIVSVNVISEKHPLKVKNFGIWLRYDSRSGTHNMYKEYRETSRVAAVEAMYADMAARHRARFRSIHILKVVELEKTEDVKRPYIKQLVAKNLSFPLPHRVPKISTKKIFSAKRPSTFS

Entity 22 polymer

描述 description	60S ribosomal protein l21-like protein
分子量 formula_weight	18159.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	LT

MGHAAGLRSGTRYAFSRGFRKHGQIPLSTYLRTYRVGDIVDIKVNGAVQKGMPHKFYHGKTGVVYNVTKSAVGVIVYKRVKHRYIEKRINVRIEHVKPSRSREDFLRRVKENAELKKKAKAEGVPVQLKRQPAMPREAHTVSIADNKPVTLAPVAYETTI

Entity 23 polymer

描述 description	60S ribosomal protein L25-like protein
分子量 formula_weight	17175.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	LX

MAPKDVKKGGASKAAKGAQAKKAAQAALKGVHSHKKTKVRKSTTFHRPKTLVLSRAPKYPRKSIPHEPRLDEHKIIIHPLNTEGALKKIEEQNTLVFIVDVKANKAQIKQALKKLYDIDTVKINTLIRPDGTKKAFARLTPDVDALDIAATKLGLV

Entity 24 polymer

描述 description	60S ribosomal protein L26-like protein
分子量 formula_weight	15565.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	LY

MKVRPTVSSSRRKARKAHFSAPSSVRRVIMSAPLSKELREKYNVRSIPIRKGDEVQIVRGAHKDKEGKVTSVYRLKYVIHVERVTREKATGQTVPIGIHPSNVVITKLHLDKDRENILARIKAGREQVAKAKGKKTAA

Entity 25 polymer

描述 description	60S ribosomal protein L32-like protein
分子量 formula_weight	15096.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Le

MVAAKKHVPIVKKRTKRFMRHQSDRFKCLDSAWRKPKGIDNRVRRRFKGNLAMPSIGYGSNKKTKHMMPSGHKAFLVHNVKDVELLLMHNRTYAAEIAHNVSSRKRIDIITRAKQLGVKVTNAKAKVTTEV

Entity 26 polymer

描述 description	60S ribosomal protein l33-like protein
分子量 formula_weight	12330.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Lf

MPSEAGHRLYVKGRHLSYQRGRRNTHPKTSLIKIEGVDDTAAANFYLGKRVAYVYRAQKEVRGTKIRVIWGKITRPHGNSGVVRAKFTHPLPARSFGASVRIMLYPSSI

Entity 27 polymer

描述 description	dolichyl-diphosphooligosaccharide--protein glycotransferase
分子量 formula_weight	105169.2 kDa
EC 编号 ec_number	2.4.99.18
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Lh

MSSNGKVKAGQLWSKNKEELTKILGELKTELSQLRIQKISSSGAKLNKIHDLRKSIARVLTVINAKQRAQLRLFYKNKKYLPLDLRPKLTRALRRRLSKEDASRVLEKTKKRLTHFPQRKYAVKAATYSEGPALSIYHSHQREARCSLVQCCTFEPELRESLLLLPVAASLKGPNFTRRERQHRNPSEATMSAAEPLKLLASAGKGKSTRSVLRVAILVLIAGAAVASRLFSVIRFESIIHEFDPWFNFRATKYLVANGFYKFWDWFDDRTWHPLGRVTGGTLYPGLMVTSGVIYHLLRFLTVPVDIRNICVLLAPGFSGLTAIAAYLLTNEMTTSPSAGLLAAAFMGIAPGYISRSVAGSYDNEAIAIFLLVFTFFLWIKALKQGSMLWGALCALFYGYMVASWGGYAFITCLLPLHSFVLICMGRYSTRLYVAYTTWYALGTLASMQIPFVGFLPVKTSEHMPALGIFGFLQLLAFLDYVRSTISSRQFQTFLWLFAGGIFGLGLLGLVIATSAGLIAPWSGRFYSLWDTGYAKIHIPIIASVSEHQPTAWPAFFFDLNMLVWLFPVGVYLCFQQLGDEHVFIIVYALFGSYFAGVMVRLMLTLTPVVCVAAAIAFSSLLDTYLNLKTPNPGQAQATEDAGKKKSGLKAASKPAVGVYALWGKTMMISGLTIYLLLFVLHCTWVTSNAYSSPSVVLASRLPDGSQHIIDDYREAYQWLRQNTREDAKIMSWWDYGYQIGGMADRPTLVDNNTWNNTHIATVGKAMASREEVSYPIMRQHEVDYVLVVFGGLLGYSGDDINKFLWMVRIAEGIWPDEVSERAFFTPRGEYRVDAEATDTMKNSLMYKMCYYNYNNLFPPGQAVDRMRGVRLPEVGPTLNTLEEAFTSENWIIRIYKVKDLDNLGRDHASAAAFERGHKKKKATKKRGPRVLRVE

Entity 28 polymer

描述 description	60S ribosomal protein L36
分子量 formula_weight	12784.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Li

MSEDATPKAPVERTGLIRGLNKGHKTTRRVLKERPSRRKGAQSKRTQFVRSLIREVAGLAPYERRVIELLRNGKDKRARKFSKKKLGTFGRAKRKCEELQRIIAESRRAH

Entity 29 polymer

描述 description	Ribosomal protein L37
分子量 formula_weight	10660.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Lj

MTKGTSSFGKRHNKTHGLCRRCGRRSLHNQKKVCASCGYPAAKTRKYNWSEKAKRRKVTGTGRMRYLSTVPRRFKNGFRTGVPKGARGPAVSTSS

Entity 30 polymer

描述 description	Ribosomal RNA-processing protein 1
分子量 formula_weight	32128.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Cc

MTSQEKSMPFIKHLASSDRKVRTAALNSLHAFLSARQVASALTTLDVLKLWKGLFYALWMCDRAIPQQNLCNELADLIWQLPRESVATWLRGFWATMAREWTGIDVLRMEKFLLLVRRVLGASFKWMKKDGSLGKRTHDGQEKAVKTGAWDQSKVDEVLGLLAEWPFSLAEEVRITQSSEKGGEIVQKIPVGMRLHVLDIWVDEVERVGLLNEDEEEARMIVQRISDMVDALEQTTKSPAVRTRSKDSLGDDRLPANRRPNSSQDHDTKDMGDSDSWEGFDD

Entity 31 polymer

描述 description	Brix domain-containing protein
分子量 formula_weight	49471.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Cd

MAASKSKDLSRVSLARTIGNKLKRQQLYIQAKKANSRAKHEERHRRRKEEAKNPELKQKRLEKNQPASIDKKRIWDDLDDDSLGAVVDVAELKRRRLEVAEAAAAAEADAAAREDQEVDDKNDENEKRDDDIDSMLGSDDEDEEDEGAKKEKEEEMQRQRAQRQPSIAPSTTSTNLDLTPDSLTSQFPNLFADEPPPVPKILVTTGINGTIHKEAEEIASIFPNATYIRRTAHRYAYKYSVREIAKFAKNRGYTALLVVQEDQKRPSRLVVVHLNGEGVPPGPTLTYTIRNYAPGKAIPGHGRATNHYPELLLNNFKTPLGLLAAKSMHILFPSKPELAGRQVVTLHNQRDYIFFRRHRYVFREARPTEKNVVGADGKEMEGVKGIRAGLQEIGPRMTLKLRRVDKGVGRAGSEGPDAVKWEWKAKMEKKRTRFNL

Entity 32 polymer

描述 description	Protein MAK16
分子量 formula_weight	39464.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Ce

MSDEIVWQIINQQFCAFKLKTTKGQNFCRNEYSVSGLCNRQSCPLANSRYATVRQSPKGTIYLYIKTIERSHMPSKWWEKIKLSQNYQKALEQIDQRLQFFPKFLIHKCKQRLTRLVQVAIRMRRIAAEEARLGEKLVPKMAPKIKKREEARERKALAAAKLERTIERELLDRLRQGAYGDQPLNCNPQIWKKVLNALEAEGEGVRDKDHDKGIEADEEGLESAEESEVEYEREEENEEDNVVEYVSDFEESEDELGDLEDWLGSDDEEFDDEDDDDEDDDDEDDDESEEDDEEELKKKKAGDKRKRGKAVKPKARKRKELEIEREEADRAKLLAF

Entity 33 non-polymer

描述 description	ZINC ION
分子量 formula_weight	65.4 kDa
来源方法 src_method	syn
拷贝数 entity_count	2

▶

7. 引用文献 (1)

▶

分辨率 resolution	3.00 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	83243

fit	/app/data/saxs_computation/i9/8i9p/8i9p.fit
pddf	/app/data/pr_computation/i9/8i9p/8i9p.pr.out
profile	/app/data/saxs_computation/i9/8i9p/8i9p.dat

Cryo-EM structure of a Chaetomium thermophilum pre-60S ribosomal subunit - State Mak16

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

冷冻电镜 EM 3D

5. 实体与聚合体信息 (33)

Entity 1 polymer

聚合体 Polymer

Entity 2 polymer

聚合体 Polymer

Entity 3 polymer

聚合体 Polymer

Entity 4 polymer

聚合体 Polymer

Entity 5 polymer

聚合体 Polymer

Entity 6 polymer

聚合体 Polymer

Entity 7 polymer

聚合体 Polymer

Entity 8 polymer

聚合体 Polymer

Entity 9 polymer

聚合体 Polymer

Entity 10 polymer

聚合体 Polymer

Entity 11 polymer

聚合体 Polymer

Entity 12 polymer

聚合体 Polymer

Entity 13 polymer

聚合体 Polymer

Entity 14 polymer

聚合体 Polymer

Entity 15 polymer

聚合体 Polymer

Entity 16 polymer

聚合体 Polymer

Entity 17 polymer

聚合体 Polymer

Entity 18 polymer

聚合体 Polymer

Entity 19 polymer

聚合体 Polymer

Entity 20 polymer

聚合体 Polymer

Entity 21 polymer

聚合体 Polymer

Entity 22 polymer

聚合体 Polymer

Entity 23 polymer

聚合体 Polymer

Entity 24 polymer

聚合体 Polymer

Entity 25 polymer

聚合体 Polymer

Entity 26 polymer

聚合体 Polymer

Entity 27 polymer

聚合体 Polymer

Entity 28 polymer

聚合体 Polymer

Entity 29 polymer

聚合体 Polymer

Entity 30 polymer

聚合体 Polymer

Entity 31 polymer

聚合体 Polymer

Entity 32 polymer

聚合体 Polymer

Entity 33 non-polymer

7. 引用文献 (1)

8. 文件与曲线 (10)

Files (7)

Curves (3)