SAXSdb — 8jzd

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Nitrate reductase molybdenum cofactor assembly chaperone NarJ

Escherichia coli K-12

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 2	Respiratory nitrate reductase 1 alpha chain × 1 （P09152）	Consistent with protein count
2	Protein heterocomplex	Heteromer	Protein 2	Respiratory nitrate reductase 1 alpha chain × 1 （P09152）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	NARJ_ECOLI
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–183； UniProt 1–183 Author chain C； PDBConstruct 1–183； UniProt 1–183

Respiratory nitrate reductase 1 alpha chain

OrganismNot specified

UniProt P09152

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 2	Nitrate reductase molybdenum cofactor assembly chaperone NarJ × 1 （P0AF26）	Consistent with protein count
2	Protein heterocomplex	Heteromer	Protein 2	Nitrate reductase molybdenum cofactor assembly chaperone NarJ × 1 （P0AF26）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	NARG_ECOLI
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain B； PDBConstruct 1–15； UniProt 1–15 Author chain D； PDBConstruct 1–15； UniProt 1–15

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	8jzd
Deposition date deposition_date	2023-07-05
Last revision last_revision	2024-05-15
Structure title title	Crystal structure of Escherichia coli NarJ in complex with the signal peptide of E. coli NarG
Keywords keywords	redox enzyme maturation protein, chaperone, NarJ subfamily, signal peptide; CHAPERONE
Experimental Method method	X-RAY DIFFRACTION

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

8jzd__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

8jzd__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

8jzd__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	17.06 Å
Rg (electron density)	15.44 Å
Total Rg	16.56 Å
Atom count	1373
Residues	179
Excluded volume	24535 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`8jzd__assembly_1__model_1`	dimeric （2）	Success	4.1.3-1-20251215 (887e7ef)	View Download
2	1	`8jzd__assembly_2__model_1`	dimeric （2）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (2)

Entity 1 Entity 1 polymer

Description description	Nitrate reductase molybdenum cofactor assembly chaperone NarJ
Molecular weight formula_weight	21781.7 kDa
Source method src_method	man
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,C
Atom count atom_count	2719

MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALGIFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHGLQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDLLLKLANTAIDSDKVAEKIADEARPGAHHHHHH

Source Source

Source organism organism	Escherichia coli K-12
NCBI taxonomy ID ncbi_taxonomy_id	83333
Host organism host_organism	Escherichia coli BL21(DE3)

Entity 2 Entity 2 polymer

Description description	Respiratory nitrate reductase 1 alpha chain
Molecular weight formula_weight	1956.3 kDa
EC 编号 ec_number	1.7.5.1
Source method src_method	syn
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	B,D

MSKFLDRFRYFKQKG

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Unit-cell length a length_a	185.443 Å
Unit-cell length b length_b	185.443 Å
Unit-cell length c length_c	37.943 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	120.00 °
Molecules per unit cell Z z_pdb	18

Space group space_group	H 3
Space-group number int_tables_number	146
Crystal system crystal_system	trigonal

Resolution resolution	2.450 Å
Low-resolution limit resolution_low	28.880 Å
R factor r_factor	0.2103
R_work r_work	0.2081
R_free r_free	0.2531

Crystal structure of Escherichia coli NarJ in complex with the signal peptide of E. coli NarG

1. Protein Identity and Related Structures Protein Identity & Related Structures

Nitrate reductase molybdenum cofactor assembly chaperone NarJ

State in the Current Structure

Other States of the Same Protein in the Database

Respiratory nitrate reductase 1 alpha chain

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

8jzd__assembly_1__model_1

8jzd__assembly_1__model_1 | I(q)

8jzd__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (2)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 polymer

Polymer Polymer

7. Citations (1)

Crystal structure of Escherichia coli NarJ in complex with the signal peptide of E. coli NarG

1. Protein Identity and Related Structures Protein Identity & Related Structures

Nitrate reductase molybdenum cofactor assembly chaperone NarJ

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Respiratory nitrate reductase 1 alpha chain

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

8jzd__assembly_1__model_1

8jzd__assembly_1__model_1 | I(q)

8jzd__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (2)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 polymer

Polymer Polymer

7. Citations (1)