Titin
Homo sapiens
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Protein homooligomer Homooligomer Protein × 6 PDB declaration: hexameric（6） Consistent with protein copy count | Chain A； UniProt 14217–14319 Chain B； UniProt 14217–14319 Chain C； UniProt 14217–14319 Chain D； UniProt 14217–14319 Chain E； UniProt 14217–14319 Chain F； UniProt 14217–14319 | Mutation：R15908H | No other associated polymer | X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；293 K；10mM Tris-HCl PH 7.5 NaCl 0.5mM DTT 0.1M sodium acetate pH 4.5 0.2M Lithium sulphate 50% v/v PEG 400 | Resolution 2.10 Å R-free 0.257 |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 8OIY | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1TNM TERTIARY STRUCTURE OF AN IMMUNOGLOBULIN-LIKE DOMAIN FROM THE GIANT MUSCLE PROTEIN TITIN: A NEW MEMBER OF THE I SET Deposited 1995-01-17 | Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
33489–33579（91 aa）
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR mmCIF provides none of the parsed conditions | Resolution not provided |
| 1TNN Tertiary structure of an immunoglobulin-like domain from the giant muscle protein titin: a new member of the I set Deposited 1995-01-17 | Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
33489–33579（91 aa）
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR mmCIF provides none of the parsed conditions | Resolution not provided |
| 1WAA IG27 protein domain Deposited 2004-10-25 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
12801–12889（89 aa）
Fragment：IG DOMAIN, RESIDUES 12801-12889
Chain B
12801–12889（89 aa）
Fragment：IG DOMAIN, RESIDUES 12801-12889
Chain C
12801–12889（89 aa）
Fragment：IG DOMAIN, RESIDUES 12801-12889
Chain D
12801–12889（89 aa）
Fragment：IG DOMAIN, RESIDUES 12801-12889
Chain E
12801–12889（89 aa）
Fragment：IG DOMAIN, RESIDUES 12801-12889
Chain F
12801–12889（89 aa）
Fragment：IG DOMAIN, RESIDUES 12801-12889
|
Not recorded | ZN ZINC ION × 24 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.80 Å R-free 0.268 |
| 2A38 Crystal structure of the N-Terminus of titin Deposited 2005-06-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–194（194 aa）
Fragment：Ig-like 1/2, residues 1-194
|
Not recorded | CD CADMIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；MES, Cadmium sulphate, Sodium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.255 |
| 2A38 Crystal structure of the N-Terminus of titin Deposited 2005-06-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–194（194 aa）
Fragment：Ig-like 1/2, residues 1-194
|
Not recorded | CD CADMIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；MES, Cadmium sulphate, Sodium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.255 |
| 2A38 Crystal structure of the N-Terminus of titin Deposited 2005-06-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
1–194（194 aa）
Fragment：Ig-like 1/2, residues 1-194
|
Not recorded | CD CADMIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；MES, Cadmium sulphate, Sodium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.255 |
| 2BK8 M1 domain from titin Deposited 2005-02-11 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
32497–32590（94 aa）
Fragment：RESIDUES 25073-25166 (IG-LIKE DOMAIN)
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7；2.2 M AMMONIUM SULFATE 0.1 M BIS TRIS PH 7.0
|
Resolution 1.69 Å R-free 0.234 |
| 2F8V Structure of full length telethonin in complex with the N-terminus of titin Deposited 2005-12-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
1–196（196 aa）
Fragment：Domains Z1Z2, residues 1-196
Chain B
1–196（196 aa）
Fragment：Domains Z1Z2, residues 1-196
|
Not recorded | SO4 SULFATE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.45；298 K；PEG35000, LITHIUM SULPHATE, ACETATE BUFFER, pH 4.45, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.75 Å R-free 0.287 |
| 2F8V Structure of full length telethonin in complex with the N-terminus of titin Deposited 2005-12-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain C
1–196（196 aa）
Fragment：Domains Z1Z2, residues 1-196
Chain D
1–196（196 aa）
Fragment：Domains Z1Z2, residues 1-196
|
Not recorded | SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.45；298 K；PEG35000, LITHIUM SULPHATE, ACETATE BUFFER, pH 4.45, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.75 Å R-free 0.287 |
| 2ILL Anomalous substructure of Titin-A168169 Deposited 2006-10-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
31854–32047（194 aa）
Fragment：Titin-A168169
|
Not recorded | CL CHLORIDE ION × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.20 Å R-free 0.243 |
| 2J8H Structure of the immunoglobulin tandem repeat A168-A169 of titin Deposited 2006-10-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
24430–24623（194 aa）
Fragment：IG LIKE DOMAIN, RESIDUES 24430-24623
|
Not recorded | GOL GLYCEROL × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 9；AMMONIUM SULFATE, BICINE, pH 9
|
Resolution 1.99 Å R-free 0.210 |
| 2J8O Structure of the immunoglobulin tandem repeat of titin A168-A169 Deposited 2006-10-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24430–24623（194 aa）
Fragment：IG LIKE DOMAIN, RESIDUES 24430-24623
|
Not recorded | GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 9；AMMONIUM SULFATE, BICINE, pH 9
|
Resolution 2.49 Å R-free 0.300 |
| 2J8O Structure of the immunoglobulin tandem repeat of titin A168-A169 Deposited 2006-10-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
24430–24623（194 aa）
Fragment：IG LIKE DOMAIN, RESIDUES 24430-24623
|
Not recorded | GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 9；AMMONIUM SULFATE, BICINE, pH 9
|
Resolution 2.49 Å R-free 0.300 |
| 2NZI Crystal structure of domains A168-A170 from titin Deposited 2006-11-23 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
31854–32155（302 aa）
Fragment：Residues 31854-32155
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；298 K；2.1M NaCl, 0.1M MES, 0.6M Li2SO4, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.90 Å R-free 0.274 |
| 2NZI Crystal structure of domains A168-A170 from titin Deposited 2006-11-23 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
31854–32155（302 aa）
Fragment：Residues 31854-32155
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；298 K；2.1M NaCl, 0.1M MES, 0.6M Li2SO4, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.90 Å R-free 0.274 |
| 2RQ8 Solution NMR structure of titin I27 domain mutant Deposited 2009-03-05 | Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
12677–12765（89 aa）
Fragment：UNP residues 12677-12765
|
Mutation：K3E, Y9P, T42A, A78T | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 7.4；298 K；Ionic strength (raw mmCIF value) 50mM POTASSIUM PHOSPHATE；Pressure 1
NMR sample composition
1.0mM TITIN I27 mutant, 50mM potassium phosphate buffer, 99.9% D2O | 99.9% D2O
NMR sample composition
1.0mM [U-15N] TITIN I27 mutant, 50mM potassium phosphate buffer, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
1.0mM [U-13C; U-15N] TITIN I27 mutant, 50mM potassium phosphate buffer, 99.9% D2O | 99.9% D2O
|
Resolution not provided |
| 2WP3 Crystal structure of the Titin M10-Obscurin like 1 Ig complex Deposited 2009-08-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain T
34252–34350（99 aa）
Fragment：M10, RESIDUES 34252-34350
|
Not recorded | GOL GLYCEROL × 4 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7；PROTEIN COMPLEX AT 13 MG/ML 2M AMM SULPHATE, 0.1 M BIS-TRIS PH 5.5
|
Resolution 1.48 Å R-free 0.217 |
| 2WWK Crystal structure of the Titin M10-Obscurin like 1 Ig F17R mutant complex Deposited 2009-10-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain T
34252–34350（99 aa）
Fragment：M10, RESIDUES 34252-34350
|
Not recorded | GOL GLYCEROL × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5.5；0.1 M BIS-TRIS PH 5.5, 0.2 M AMMONIUM SULPHATE, 25% PEG 3350
|
Resolution 1.70 Å R-free 0.259 |
| 2WWM Crystal structure of the Titin M10-Obscurin like 1 Ig complex in space group P1 Deposited 2009-10-26 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain T
34252–34350（99 aa）
Fragment：M10, RESIDUES 34252-34350
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5；0.1 M MIB BUFFER PH 5.0, 25% PEG 1500
|
Resolution 2.30 Å R-free 0.252 |
| 2WWM Crystal structure of the Titin M10-Obscurin like 1 Ig complex in space group P1 Deposited 2009-10-26 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain D
34252–34350（99 aa）
Fragment：M10, RESIDUES 34252-34350
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5；0.1 M MIB BUFFER PH 5.0, 25% PEG 1500
|
Resolution 2.30 Å R-free 0.252 |
| 2Y9R Crystal structure of the M10 domain of Titin Deposited 2011-02-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain T
26828–26926（99 aa）
Fragment：M10 DOMAIN, RESIDUES 26828-26926
|
Not recorded | 12P DODECAETHYLENE GLYCOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.5；35% PEG 400, 0.1M BIS-TRIS PH 6.5
|
Resolution 1.90 Å R-free 0.241 |
| 3KNB Crystal structure of the titin C-terminus in complex with obscurin-like 1 Deposited 2009-11-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
34253–34350（98 aa）
Fragment：titin, C-terminal domain M10, resideus 34253-34350
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；ammonium sulfate, imidazole, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.40 Å R-free 0.195 |
| 3LCY Titin Ig tandem domains A164-A165 Deposited 2010-01-12 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
31456–31649（194 aa）
Fragment：Titin A-band tandem Ig domains A164-A165
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；0.1M Hepes PH 7.5, 10% iso-propanol
8.5% PEG20,000, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.50 Å R-free 0.240 |
| 3LCY Titin Ig tandem domains A164-A165 Deposited 2010-01-12 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
31456–31649（194 aa）
Fragment：Titin A-band tandem Ig domains A164-A165
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；0.1M Hepes PH 7.5, 10% iso-propanol
8.5% PEG20,000, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.50 Å R-free 0.240 |
| 3LCY Titin Ig tandem domains A164-A165 Deposited 2010-01-12 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
31456–31649（194 aa）
Fragment：Titin A-band tandem Ig domains A164-A165
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；0.1M Hepes PH 7.5, 10% iso-propanol
8.5% PEG20,000, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.50 Å R-free 0.240 |
| 3LCY Titin Ig tandem domains A164-A165 Deposited 2010-01-12 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
31456–31649（194 aa）
Fragment：Titin A-band tandem Ig domains A164-A165
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；0.1M Hepes PH 7.5, 10% iso-propanol
8.5% PEG20,000, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.50 Å R-free 0.240 |
| 3LPW Crystal structure of the FnIII-tandem A77-A78 from the A-band of titin Deposited 2010-02-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
22877–23070（194 aa）
|
Not recorded | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.65 Å R-free 0.208 |
| 3LPW Crystal structure of the FnIII-tandem A77-A78 from the A-band of titin Deposited 2010-02-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
22877–23070（194 aa）
|
Not recorded | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.65 Å R-free 0.208 |
| 3PUC Atomic resolution structure of titin domain M7 Deposited 2010-12-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
33774–33871（98 aa）
Fragment：domain M7 (UNP RESIDUES 33774-33871)
|
Not recorded | SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；0.1M Tris pH 8.0, 3.2M ammonium sulfate, 1mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 0.96 Å R-free 0.135 |
| 3Q5O Crystal structure of human titin domain M10 Deposited 2010-12-29 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
34253–34350（98 aa）
Fragment：domain M10 (UNP RESIDUES 34253-34350)
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；277 K；0.1M bistris p 6.8, 55% MPD, 0.2M magnesium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.05 Å R-free 0.215 |
| 3Q5O Crystal structure of human titin domain M10 Deposited 2010-12-29 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
34253–34350（98 aa）
Fragment：domain M10 (UNP RESIDUES 34253-34350)
|
Not recorded | SO4 SULFATE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；277 K；0.1M bistris p 6.8, 55% MPD, 0.2M magnesium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.05 Å R-free 0.215 |
| 3QP3 Crystal structure of titin domain M4, tetragonal form Deposited 2011-02-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
33294–33395（102 aa）
Fragment：domain M4 (UNP RESIDUES 33294-33395)
|
Not recorded | SO4 SULFATE ION × 5 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；293 K；0.2M Na-citrate pH 4.0, 1.2M (NH4)2SO4, 0.3M MgSO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.213 |
| 3QP3 Crystal structure of titin domain M4, tetragonal form Deposited 2011-02-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
33294–33395（102 aa）
Fragment：domain M4 (UNP RESIDUES 33294-33395)
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；293 K；0.2M Na-citrate pH 4.0, 1.2M (NH4)2SO4, 0.3M MgSO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.213 |
| 3QP3 Crystal structure of titin domain M4, tetragonal form Deposited 2011-02-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
33294–33395（102 aa）
Fragment：domain M4 (UNP RESIDUES 33294-33395)
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；293 K；0.2M Na-citrate pH 4.0, 1.2M (NH4)2SO4, 0.3M MgSO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.213 |
| 4C4K Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2013-09-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain T
34252–34350（99 aa）
Fragment：M10 DOMAIN, RESIDUES 34252-34350
|
Not recorded | EDO 1,2-ETHANEDIOL × 12 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8.5；0.1 M TRIS-HCL PH 8.5, 0.2 M SODIUM ACETATE, 30% PEG4000
|
Resolution 1.95 Å R-free 0.210 |
| 4JNW Bacterially expressed Titin Kinase Deposited 2013-03-16 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
32172–32492（321 aa）
Fragment：titin kinase: unp residues 32172-32492
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；VAPOR DIFFUSION, HANGING DROP
|
Resolution 2.06 Å R-free 0.204 |
| 4JNW Bacterially expressed Titin Kinase Deposited 2013-03-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
32172–32492（321 aa）
Fragment：titin kinase: unp residues 32172-32492
|
Not recorded | GOL GLYCEROL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；VAPOR DIFFUSION, HANGING DROP
|
Resolution 2.06 Å R-free 0.204 |
| 4O00 Crystal structure of the Titin A-band domain A3 Deposited 2013-12-13 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
14217–14319（103 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4；291 K；0.1M SPG buffer, 0.01M Tris-HCl, 31% PEG1500, 50mM NaCl, 1mM DTT, pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K
|
Resolution 1.85 Å R-free 0.208 |
| 4O00 Crystal structure of the Titin A-band domain A3 Deposited 2013-12-13 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
14217–14319（103 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4；291 K；0.1M SPG buffer, 0.01M Tris-HCl, 31% PEG1500, 50mM NaCl, 1mM DTT, pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K
|
Resolution 1.85 Å R-free 0.208 |
| 4QEG Crystal structure of domain I10 from titin (space group P41) Deposited 2014-05-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2880–2967（88 aa）
Fragment：domain I10
|
Not recorded | CA CALCIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；0.2 M CaCl2, 30% PEG 3350, 0.1 M Tris, 3% Isopropanol, pH 7.5, VAPOR DIFFUSION, SITTING DROP
|
Resolution 2.00 Å R-free 0.231 |
| 4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain Y
34253–34349（97 aa）
Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
|
Not recorded | CL CHLORIDE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
|
Resolution 3.30 Å R-free 0.210 |
| 4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 10 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain O
34253–34349（97 aa）
Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
|
Not recorded | NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
|
Resolution 3.30 Å R-free 0.210 |
| 4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 11 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain K
34253–34349（97 aa）
Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
|
Resolution 3.30 Å R-free 0.210 |
| 4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 12 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain U
34253–34349（97 aa）
Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
|
Resolution 3.30 Å R-free 0.210 |
| 4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 13 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain G
34253–34349（97 aa）
Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
|
Resolution 3.30 Å R-free 0.210 |
| 4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 14 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain Q
34253–34349（97 aa）
Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
|
Not recorded | CL CHLORIDE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
|
Resolution 3.30 Å R-free 0.210 |
| 4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 15 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain W
34253–34349（97 aa）
Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
|
Not recorded | CL CHLORIDE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
|
Resolution 3.30 Å R-free 0.210 |
| 4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 16 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain 2
34253–34349（97 aa）
Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
|
Resolution 3.30 Å R-free 0.210 |
| 4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 17 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
34253–34349（97 aa）
Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
|
Not recorded | CL CHLORIDE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
|
Resolution 3.30 Å R-free 0.210 |
| 4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 18 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain S
34253–34349（97 aa）
Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
|
Not recorded | CL CHLORIDE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
|
Resolution 3.30 Å R-free 0.210 |
| 4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain M
34253–34349（97 aa）
Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
|
Not recorded | CL CHLORIDE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
|
Resolution 3.30 Å R-free 0.210 |
| 4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain E
34253–34349（97 aa）
Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
|
Not recorded | CL CHLORIDE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
|
Resolution 3.30 Å R-free 0.210 |
| 4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain 8
34253–34349（97 aa）
Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
|
Resolution 3.30 Å R-free 0.210 |
| 4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 5 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain 6
34253–34349（97 aa）
Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
|
Resolution 3.30 Å R-free 0.210 |
| 4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 6 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain 0
34253–34349（97 aa）
Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
|
Not recorded | CL CHLORIDE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
|
Resolution 3.30 Å R-free 0.210 |
| 4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 7 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain I
34253–34349（97 aa）
Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
|
Resolution 3.30 Å R-free 0.210 |
| 4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 8 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
34253–34349（97 aa）
Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
|
Resolution 3.30 Å R-free 0.210 |
| 4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 9 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain 4
34253–34349（97 aa）
Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
|
Resolution 3.30 Å R-free 0.210 |
| 5BS0 MAGE-A3 Reactive TCR in complex with Titin Epitope in HLA-A1 Deposited 2015-06-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 5 PDB declaration: pentameric |
Chain C
24337–24345（9 aa）
Fragment：UNP residues 24337-24345
|
Not recorded | GOL GLYCEROL × 1 SO4 SULFATE ION × 11 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；291 K；0.2M Ammonium Chloride, 0.1M MES, 20% PEG 4000
|
Resolution 2.40 Å R-free 0.262 |
| 5JDD Crystal structure of I9-I11 tandem from titin (P212121) Deposited 2016-04-16 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2795–3053（259 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；292 K；0.1 M Bis-Tris Propane pH 8.5, 20% [w/v] PEG 3350, 0.2 M sodium acetate
|
Resolution 1.53 Å R-free 0.197 |
| 5JDE Crystal structure of I9-I11 tandem from titin (P1) Deposited 2016-04-16 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2795–3053（259 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；292 K；0.1 M Tris HCl pH 8.5, 30% [w/v] PEG 4000, 0.2 M MgCl2
|
Resolution 1.90 Å R-free 0.219 |
| 5JDE Crystal structure of I9-I11 tandem from titin (P1) Deposited 2016-04-16 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
2795–3053（259 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；292 K；0.1 M Tris HCl pH 8.5, 30% [w/v] PEG 4000, 0.2 M MgCl2
|
Resolution 1.90 Å R-free 0.219 |
| 5JDJ Crystal structure of domain I10 from titin in space group P212121 Deposited 2016-04-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2880–2967（88 aa）
Chain L
2880–2967（88 aa）
|
Not recorded | CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；292 K；0.2 M CaCl2, 0.1 M Tris pH 7.5, 25% [w/v] PEG 8000
|
Resolution 1.74 Å R-free 0.224 |
| 5JDJ Crystal structure of domain I10 from titin in space group P212121 Deposited 2016-04-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain B
2880–2967（88 aa）
Chain P
2880–2967（88 aa）
|
Not recorded | CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；292 K；0.2 M CaCl2, 0.1 M Tris pH 7.5, 25% [w/v] PEG 8000
|
Resolution 1.74 Å R-free 0.224 |
| 5JDJ Crystal structure of domain I10 from titin in space group P212121 Deposited 2016-04-16 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain C
2880–2967（88 aa）
Chain M
2880–2967（88 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；292 K；0.2 M CaCl2, 0.1 M Tris pH 7.5, 25% [w/v] PEG 8000
|
Resolution 1.74 Å R-free 0.224 |
| 5JDJ Crystal structure of domain I10 from titin in space group P212121 Deposited 2016-04-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain D
2880–2967（88 aa）
Chain E
2880–2967（88 aa）
|
Not recorded | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；292 K；0.2 M CaCl2, 0.1 M Tris pH 7.5, 25% [w/v] PEG 8000
|
Resolution 1.74 Å R-free 0.224 |
| 5JDJ Crystal structure of domain I10 from titin in space group P212121 Deposited 2016-04-16 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 5 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain F
2880–2967（88 aa）
Chain N
2880–2967（88 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；292 K；0.2 M CaCl2, 0.1 M Tris pH 7.5, 25% [w/v] PEG 8000
|
Resolution 1.74 Å R-free 0.224 |
| 5JDJ Crystal structure of domain I10 from titin in space group P212121 Deposited 2016-04-16 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 6 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain G
2880–2967（88 aa）
Chain J
2880–2967（88 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；292 K；0.2 M CaCl2, 0.1 M Tris pH 7.5, 25% [w/v] PEG 8000
|
Resolution 1.74 Å R-free 0.224 |
| 5JDJ Crystal structure of domain I10 from titin in space group P212121 Deposited 2016-04-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 7 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain H
2880–2967（88 aa）
Chain I
2880–2967（88 aa）
|
Not recorded | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；292 K；0.2 M CaCl2, 0.1 M Tris pH 7.5, 25% [w/v] PEG 8000
|
Resolution 1.74 Å R-free 0.224 |
| 5JDJ Crystal structure of domain I10 from titin in space group P212121 Deposited 2016-04-16 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 8 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain K
2880–2967（88 aa）
Chain O
2880–2967（88 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；292 K；0.2 M CaCl2, 0.1 M Tris pH 7.5, 25% [w/v] PEG 8000
|
Resolution 1.74 Å R-free 0.224 |
| 5JOE Crystal structure of I81 from titin Deposited 2016-05-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
9582–9671（90 aa）
Fragment：I81 domain
|
Not recorded | IPA ISOPROPYL ALCOHOL × 1 EDO 1,2-ETHANEDIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；292.15 K；0.1M Tris pH 8.5; 30% w/v PEG 4000
|
Resolution 2.00 Å R-free 0.218 |
| 6DL4 Human Titin ZIg10 Deposited 2018-05-31 | Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1821–1931（111 aa）
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 7.5；298 K；Ionic strength (raw mmCIF value) 50；Pressure 1
NMR sample composition
20 mM TRIS, 50 mM sodium chloride, 1 mM [U-99% 13C; U-99% 15N] Human Titin ZIg10, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 6FWX Chimeric titin Z1Z2-Z1Z2 tandem (Z1212) functionalized with a GRGDS exogenous peptide from fibronectin Deposited 2018-03-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–196（196 aa）
Chain A
2–47（46 aa）
Chain A
52–196（145 aa）
|
Not recorded | EDO 1,2-ETHANEDIOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；10% [v/v] isopropanol, 100 mM Na2HPO4 pH 4.2, 200 mM LiSO4
|
Resolution 3.00 Å R-free 0.226 |
| 6FWX Chimeric titin Z1Z2-Z1Z2 tandem (Z1212) functionalized with a GRGDS exogenous peptide from fibronectin Deposited 2018-03-07 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–196（196 aa）
Chain B
2–47（46 aa）
Chain B
52–196（145 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；10% [v/v] isopropanol, 100 mM Na2HPO4 pH 4.2, 200 mM LiSO4
|
Resolution 3.00 Å R-free 0.226 |
| 6H4L Structure of Titin M4 trigonal form Deposited 2018-07-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
33294–33395（102 aa）
|
Not recorded | ZN ZINC ION × 1 CL CHLORIDE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；MES pH 6.5
2M RbBr
|
Resolution 1.60 Å R-free 0.221 |
| 6HCI Crystal structure of titin M3 domain Deposited 2018-08-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
32712–32816（105 aa）
Chain B
32712–32816（105 aa）
Chain C
32712–32816（105 aa）
Chain D
32712–32816（105 aa）
|
Not recorded | SO4 SULFATE ION × 8 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；0.2 M ammonium sulfate
23% w/v polyethylene glycol 4000
|
Resolution 2.12 Å R-free 0.245 |
| 6I0Y TnaC-stalled ribosome complex with the titin I27 domain folding close to the ribosomal exit tunnel Deposited 2018-10-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 36-meric |
Chain z
14318–14406（89 aa）
|
Not recorded | MG MAGNESIUM ION × 143 ZN ZINC ION × 1 TRP TRYPTOPHAN × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 6SDB Chimeric titin Z1Z2 functionalized with a KLER exogenous peptide from decorin Deposited 2019-07-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–45（43 aa）
Chain A
51–196（146 aa）
|
Not recorded | ACT ACETATE ION × 6 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；295 K；1 M NH4H2PO4 pH 4.6, 100 mM sodium acetate
|
Resolution 2.80 Å R-free 0.234 |
| 6SDB Chimeric titin Z1Z2 functionalized with a KLER exogenous peptide from decorin Deposited 2019-07-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
3–45（43 aa）
Chain B
51–196（146 aa）
|
Not recorded | ACT ACETATE ION × 1 SO4 SULFATE ION × 1 EDO 1,2-ETHANEDIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；295 K；1 M NH4H2PO4 pH 4.6, 100 mM sodium acetate
|
Resolution 2.80 Å R-free 0.234 |
| 6SDB Chimeric titin Z1Z2 functionalized with a KLER exogenous peptide from decorin Deposited 2019-07-26 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 3 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
3–45（43 aa）
Chain C
51–196（146 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；295 K；1 M NH4H2PO4 pH 4.6, 100 mM sodium acetate
|
Resolution 2.80 Å R-free 0.234 |
| 6SDB Chimeric titin Z1Z2 functionalized with a KLER exogenous peptide from decorin Deposited 2019-07-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
3–45（43 aa）
Chain D
51–196（146 aa）
|
Not recorded | ACT ACETATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；295 K；1 M NH4H2PO4 pH 4.6, 100 mM sodium acetate
|
Resolution 2.80 Å R-free 0.234 |
| 6SDB Chimeric titin Z1Z2 functionalized with a KLER exogenous peptide from decorin Deposited 2019-07-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 5 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain E
3–45（43 aa）
Chain E
51–196（146 aa）
|
Not recorded | ACT ACETATE ION × 3 PO4 PHOSPHATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；295 K；1 M NH4H2PO4 pH 4.6, 100 mM sodium acetate
|
Resolution 2.80 Å R-free 0.234 |
| 6YGN Titin kinase and its flanking domains Deposited 2020-03-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
30596–31144（549 aa）
|
Not recorded | EDO 1,2-ETHANEDIOL × 1 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293.15 K；30% PEG 8K, 0.1 MES pH6.5
|
Resolution 2.40 Å R-free 0.228 |
| 6YGN Titin kinase and its flanking domains Deposited 2020-03-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
30596–31144（549 aa）
|
Not recorded | EDO 1,2-ETHANEDIOL × 1 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293.15 K；30% PEG 8K, 0.1 MES pH6.5
|
Resolution 2.40 Å R-free 0.228 |
| 7AHS titin-N2A Ig81-Ig83 Deposited 2020-09-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
9582–9851（270 aa）
|
Not recorded | EDO 1,2-ETHANEDIOL × 5 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293.15 K；25 % w/v Polyethylene glycol 3,350, 100 mM BIS-TRIS pH 5.5, 200 mM lithium sulfate
|
Resolution 2.05 Å R-free 0.235 |
| 7AHS titin-N2A Ig81-Ig83 Deposited 2020-09-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
9582–9851（270 aa）
|
Not recorded | EDO 1,2-ETHANEDIOL × 7 SO4 SULFATE ION × 6 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293.15 K；25 % w/v Polyethylene glycol 3,350, 100 mM BIS-TRIS pH 5.5, 200 mM lithium sulfate
|
Resolution 2.05 Å R-free 0.235 |
| 7AHS titin-N2A Ig81-Ig83 Deposited 2020-09-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
9582–9851（270 aa）
|
Not recorded | EDO 1,2-ETHANEDIOL × 3 SO4 SULFATE ION × 2 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293.15 K；25 % w/v Polyethylene glycol 3,350, 100 mM BIS-TRIS pH 5.5, 200 mM lithium sulfate
|
Resolution 2.05 Å R-free 0.235 |
| 7AHS titin-N2A Ig81-Ig83 Deposited 2020-09-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
9582–9851（270 aa）
|
Not recorded | EDO 1,2-ETHANEDIOL × 4 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293.15 K；25 % w/v Polyethylene glycol 3,350, 100 mM BIS-TRIS pH 5.5, 200 mM lithium sulfate
|
Resolution 2.05 Å R-free 0.235 |
| 7NIP titin N2A unique sequence (UN2A) core Deposited 2021-02-13 | Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
8578–8617（40 aa）
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 7.5；298 K；Ionic strength (raw mmCIF value) 100；Pressure 101300
NMR sample composition
30 mM sodium phosphate, 100 mM sodium chloride, 95% H2O/5% D2O | 95% H2O/5% D2O
|
Resolution not provided |
| 8BNQ Crystal structure of the FnIII-tandem A84-A86 from the A-band of titin Deposited 2022-11-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
23562–23862（301 aa）
|
Not recorded | EDO 1,2-ETHANEDIOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277.15 K；5% [v/v] polyethylene glycol 400 and 40% [v/v] 2-methyl-2,4-pentanediol (MPD)
|
Resolution 2.30 Å R-free 0.258 |
| 8BNQ Crystal structure of the FnIII-tandem A84-A86 from the A-band of titin Deposited 2022-11-14 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
23562–23862（301 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277.15 K；5% [v/v] polyethylene glycol 400 and 40% [v/v] 2-methyl-2,4-pentanediol (MPD)
|
Resolution 2.30 Å R-free 0.258 |
| 8BVO Titin I110-I111 FnIII tandem from the MIR region (I/A5-I/A6) Deposited 2022-12-05 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
14415–14609（195 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；291.15 K；24% (w/v) PEG 1500, 20% (w/v) glycerol
|
Resolution 2.55 Å R-free 0.287 |
| 8BW6 Titin FnIII-domain I110 (I/A6) from the MIR region Deposited 2022-12-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
14415–14509（95 aa）
|
Not recorded | EDO 1,2-ETHANEDIOL × 7 PEG DI(HYDROXYETHYL)ETHER × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；291.15 K；0.1M Tris pH8 30% PEG 300
|
Resolution 1.95 Å R-free 0.254 |
| 8BXR Titin FnIII-domain I109-I111 (I/A4-A/A6) from the MIR region Deposited 2022-12-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
14319–14615（297 aa）
|
Not recorded | EDO 1,2-ETHANEDIOL × 6 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；291.15 K；0.1M MES pH 6, 10% [w/v] PEG 6000
|
Resolution 2.70 Å R-free 0.271 |
| 8G4L Cryo-EM structure of the human cardiac myosin filament Deposited 2023-02-10 | Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 123 PDB declaration: 123-meric |
Chain am
22784–23867（1084 aa）
Chain an
22784–23867（1084 aa）
Chain bm
22784–23867（1084 aa）
Chain bn
22784–23867（1084 aa）
Chain m
22784–23867（1084 aa）
Chain n
22784–23867（1084 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.40 Å |
| 8OMW Crystal structure of the titin domain Fn3-20 Deposited 2023-03-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
16525–16630（106 aa）
|
Not recorded | CL CHLORIDE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；10mM Tris-HCl pH 7.5
50mM NaCl
0.5mM DTT
0.1M TBG pH 9.0
25% w/v PEG 1500
|
Resolution 1.05 Å R-free 0.177 |
| 8OQ9 Crystal structure of the titin domain Fn3-56 Deposited 2023-04-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21497–21598（102 aa）
|
Not recorded | ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；10mM Tris-HCl pH 7.5
50mM NaCl
0.5mM DTT
0.1M MES
10mM ZnCl2
20% (w/v) PEG6000
|
Resolution 1.65 Å R-free 0.192 |
| 8OQ9 Crystal structure of the titin domain Fn3-56 Deposited 2023-04-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
21497–21598（102 aa）
|
Not recorded | ZN ZINC ION × 3 CL CHLORIDE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；10mM Tris-HCl pH 7.5
50mM NaCl
0.5mM DTT
0.1M MES
10mM ZnCl2
20% (w/v) PEG6000
|
Resolution 1.65 Å R-free 0.192 |
| 8OQ9 Crystal structure of the titin domain Fn3-56 Deposited 2023-04-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
21497–21598（102 aa）
|
Not recorded | ZN ZINC ION × 1 CL CHLORIDE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；10mM Tris-HCl pH 7.5
50mM NaCl
0.5mM DTT
0.1M MES
10mM ZnCl2
20% (w/v) PEG6000
|
Resolution 1.65 Å R-free 0.192 |
| 8ORL Crystal structure of the S23226G missense variant of titin domain Fn3-56 Deposited 2023-04-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21497–21598（102 aa）
|
Not recorded | ZN ZINC ION × 1 CL CHLORIDE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；10mM Tris-HCl pH 7.5
50mM NaCl
0.5mM DTT
0.1M MES
10mM ZnCl2
20% (w/v) PEG6000
|
Resolution 1.43 Å R-free 0.194 |
| 8ORL Crystal structure of the S23226G missense variant of titin domain Fn3-56 Deposited 2023-04-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
21497–21598（102 aa）
|
Not recorded | ZN ZINC ION × 3 CL CHLORIDE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；10mM Tris-HCl pH 7.5
50mM NaCl
0.5mM DTT
0.1M MES
10mM ZnCl2
20% (w/v) PEG6000
|
Resolution 1.43 Å R-free 0.194 |
| 8ORL Crystal structure of the S23226G missense variant of titin domain Fn3-56 Deposited 2023-04-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
21497–21598（102 aa）
|
Not recorded | ZN ZINC ION × 1 CL CHLORIDE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；10mM Tris-HCl pH 7.5
50mM NaCl
0.5mM DTT
0.1M MES
10mM ZnCl2
20% (w/v) PEG6000
|
Resolution 1.43 Å R-free 0.194 |
| 8OS3 Crystal structure of the titin domain Fn3-11 Deposited 2023-04-17 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
15210–15313（104 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；30mM HEPES pH 7.5
50mM NaCl
0.5mM DTT
100mM sodium acetate pH 4.5
200mM Lithium sulphate
50% v/v PEG 400
|
Resolution 1.68 Å R-free 0.208 |
| 8OSD Crystal structure of the titin domain Fn3-49 Deposited 2023-04-18 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
20511–20616（106 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；30mM HEPES pH 7.5
150mM NaCl
1mM DTT
100mM Potassium thiocyanate
30% w/v PEG MME2000
|
Resolution 1.70 Å R-free 0.260 |
| 8OSD Crystal structure of the titin domain Fn3-49 Deposited 2023-04-18 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
20511–20616（106 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；30mM HEPES pH 7.5
150mM NaCl
1mM DTT
100mM Potassium thiocyanate
30% w/v PEG MME2000
|
Resolution 1.70 Å R-free 0.260 |
| 8OT5 Crystal structure of the titin domain Fn3-85 Deposited 2023-04-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
25430–25531（102 aa）
|
Not recorded | NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；30mM HEPES pH 7.5
150mM NaCl
1mM DTT
100mM Bis-Tris propane pH 6.5
200mM sodium iodide
20% w/v PEG3350
|
Resolution 1.56 Å R-free 0.191 |
| 8OT5 Crystal structure of the titin domain Fn3-85 Deposited 2023-04-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
25430–25531（102 aa）
|
Not recorded | NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；30mM HEPES pH 7.5
150mM NaCl
1mM DTT
100mM Bis-Tris propane pH 6.5
200mM sodium iodide
20% w/v PEG3350
|
Resolution 1.56 Å R-free 0.191 |
| 8OT5 Crystal structure of the titin domain Fn3-85 Deposited 2023-04-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
25430–25531（102 aa）
|
Not recorded | NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；30mM HEPES pH 7.5
150mM NaCl
1mM DTT
100mM Bis-Tris propane pH 6.5
200mM sodium iodide
20% w/v PEG3350
|
Resolution 1.56 Å R-free 0.191 |
| 8OT5 Crystal structure of the titin domain Fn3-85 Deposited 2023-04-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
25430–25531（102 aa）
|
Not recorded | NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；30mM HEPES pH 7.5
150mM NaCl
1mM DTT
100mM Bis-Tris propane pH 6.5
200mM sodium iodide
20% w/v PEG3350
|
Resolution 1.56 Å R-free 0.191 |
| 8OT5 Crystal structure of the titin domain Fn3-85 Deposited 2023-04-20 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 5 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain E
25430–25531（102 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；30mM HEPES pH 7.5
150mM NaCl
1mM DTT
100mM Bis-Tris propane pH 6.5
200mM sodium iodide
20% w/v PEG3350
|
Resolution 1.56 Å R-free 0.191 |
| 8OT5 Crystal structure of the titin domain Fn3-85 Deposited 2023-04-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 6 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain F
25430–25531（102 aa）
|
Not recorded | CL CHLORIDE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；30mM HEPES pH 7.5
150mM NaCl
1mM DTT
100mM Bis-Tris propane pH 6.5
200mM sodium iodide
20% w/v PEG3350
|
Resolution 1.56 Å R-free 0.191 |
| 8OTY Crystal structure of the titin domain Fn3-90 Deposited 2023-04-21 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
26124–26224（101 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；30mM HEPES pH 7.5
150mM NaCl
1mM DTT
100mM Sodium acetate
200mM CaCl
20% w/v PEG6000
|
Resolution 1.90 Å R-free 0.251 |
| 8OTY Crystal structure of the titin domain Fn3-90 Deposited 2023-04-21 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
26124–26224（101 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；30mM HEPES pH 7.5
150mM NaCl
1mM DTT
100mM Sodium acetate
200mM CaCl
20% w/v PEG6000
|
Resolution 1.90 Å R-free 0.251 |
| 8OTY Crystal structure of the titin domain Fn3-90 Deposited 2023-04-21 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
26124–26224（101 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；30mM HEPES pH 7.5
150mM NaCl
1mM DTT
100mM Sodium acetate
200mM CaCl
20% w/v PEG6000
|
Resolution 1.90 Å R-free 0.251 |
| 8OTY Crystal structure of the titin domain Fn3-90 Deposited 2023-04-21 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
26124–26224（101 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；30mM HEPES pH 7.5
150mM NaCl
1mM DTT
100mM Sodium acetate
200mM CaCl
20% w/v PEG6000
|
Resolution 1.90 Å R-free 0.251 |
| 8OVU Human titin immunoglobulin-like 21 domain Deposited 2023-04-26 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3500–3598（99 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.06 M divalent ions (CaCl2, MgCl2), 0.1 M sodium HEPES, pH 7.5, 50% v/v Polyethylene glycol monomethyl ether (PEGMME) 550, PEG 20K from Morpheus HT-96 screening plate (Molecular Dimensions)
|
Resolution 1.95 Å R-free 0.239 |
| 8OVU Human titin immunoglobulin-like 21 domain Deposited 2023-04-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
3500–3598（99 aa）
|
Not recorded | MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.06 M divalent ions (CaCl2, MgCl2), 0.1 M sodium HEPES, pH 7.5, 50% v/v Polyethylene glycol monomethyl ether (PEGMME) 550, PEG 20K from Morpheus HT-96 screening plate (Molecular Dimensions)
|
Resolution 1.95 Å R-free 0.239 |
| 8P35 Mutant human titin immunoglobulin-like 21 domain - C3575S Deposited 2023-05-17 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3500–3598（99 aa）
|
Mutation：C3575S | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1 M HEPES sodium salt, pH 7.5, 25 %(w/v) PEG 2000 MME
|
Resolution 2.20 Å R-free 0.250 |
| 8P35 Mutant human titin immunoglobulin-like 21 domain - C3575S Deposited 2023-05-17 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
3500–3598（99 aa）
|
Mutation：C3575S | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1 M HEPES sodium salt, pH 7.5, 25 %(w/v) PEG 2000 MME
|
Resolution 2.20 Å R-free 0.250 |
| 8P35 Mutant human titin immunoglobulin-like 21 domain - C3575S Deposited 2023-05-17 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
3500–3598（99 aa）
|
Mutation：C3575S | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1 M HEPES sodium salt, pH 7.5, 25 %(w/v) PEG 2000 MME
|
Resolution 2.20 Å R-free 0.250 |
| 8P35 Mutant human titin immunoglobulin-like 21 domain - C3575S Deposited 2023-05-17 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
3500–3598（99 aa）
|
Mutation：C3575S | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1 M HEPES sodium salt, pH 7.5, 25 %(w/v) PEG 2000 MME
|
Resolution 2.20 Å R-free 0.250 |
| 8P35 Mutant human titin immunoglobulin-like 21 domain - C3575S Deposited 2023-05-17 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 5 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain E
3500–3598（99 aa）
|
Mutation：C3575S | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1 M HEPES sodium salt, pH 7.5, 25 %(w/v) PEG 2000 MME
|
Resolution 2.20 Å R-free 0.250 |
| 8P35 Mutant human titin immunoglobulin-like 21 domain - C3575S Deposited 2023-05-17 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 6 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain F
3500–3598（99 aa）
|
Mutation：C3575S | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1 M HEPES sodium salt, pH 7.5, 25 %(w/v) PEG 2000 MME
|
Resolution 2.20 Å R-free 0.250 |
54 other PDB entries and 123 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | TITIN_HUMAN |
| Isoform | — |
| PDB entities | 1 |
| Chains and sequence ranges | Author chain A； PDBConstruct 4–106； UniProt 14217–14319 Author chain B； PDBConstruct 4–106； UniProt 14217–14319 Author chain C； PDBConstruct 4–106； UniProt 14217–14319 Author chain D； PDBConstruct 4–106； UniProt 14217–14319 Author chain E； PDBConstruct 4–106； UniProt 14217–14319 Author chain F； PDBConstruct 4–106； UniProt 14217–14319 |
