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8g4l

Cryo-EM structure of the human cardiac myosin filament

Method: ELECTRON MICROSCOPY Dmax: 481.2 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Myosin-7

OrganismNot specified

UniProt P12883

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 123 PDB declaration: 123-meric（123） Consistent with protein copy count Chain A； UniProt 1–1935 Chain AA； UniProt 1–1935 Chain AB； UniProt 1–1935 Chain AG； UniProt 1–1935 Chain AH； UniProt 1–1935 Chain AI； UniProt 1–1935 Chain AJ； UniProt 1–1935 Chain AK； UniProt 1–1935 Chain AL； UniProt 1–1935 Chain AM； UniProt 1–1935 Chain AN； UniProt 1–1935 Chain AO； UniProt 1–1935 Chain AP； UniProt 1–1935 Chain AQ； UniProt 1–1935 Chain AR； UniProt 1–1935 Chain AS； UniProt 1–1935 Chain AT； UniProt 1–1935 Chain AU； UniProt 1–1935 Chain AV； UniProt 1–1935 Chain AW； UniProt 1–1935 Chain AX； UniProt 1–1935 Chain AY； UniProt 1–1935 Chain AZ； UniProt 1–1935 Chain B； UniProt 1–1935 Chain BA； UniProt 1–1935 Chain BB； UniProt 1–1935 Chain BG； UniProt 1–1935 Chain BH； UniProt 1–1935 Chain BI； UniProt 1–1935 Chain BJ； UniProt 1–1935 Chain BK； UniProt 1–1935 Chain BL； UniProt 1–1935 Chain BM； UniProt 1–1935 Chain BN； UniProt 1–1935 Chain BO； UniProt 1–1935 Chain BP； UniProt 1–1935 Chain BQ； UniProt 1–1935 Chain BR； UniProt 1–1935 Chain BS； UniProt 1–1935 Chain BT； UniProt 1–1935 Chain BU； UniProt 1–1935 Chain BV； UniProt 1–1935 Chain BW； UniProt 1–1935 Chain BX； UniProt 1–1935 Chain BY； UniProt 1–1935 Chain BZ； UniProt 1–1935 Chain G； UniProt 1–1935 Chain H； UniProt 1–1935 Chain I； UniProt 1–1935 Chain J； UniProt 1–1935 Chain K； UniProt 1–1935 Chain L； UniProt 1–1935 Chain M； UniProt 1–1935 Chain N； UniProt 1–1935 Chain O； UniProt 1–1935 Chain P； UniProt 1–1935 Chain Q； UniProt 1–1935 Chain R； UniProt 1–1935 Chain S； UniProt 1–1935 Chain T； UniProt 1–1935 Chain U； UniProt 1–1935 Chain V； UniProt 1–1935 Chain W； UniProt 1–1935 Chain X； UniProt 1–1935 Chain Y； UniProt 1–1935 Chain Z； UniProt 1–1935 Chain ae； UniProt 1–1935 Chain af； UniProt 1–1935 Chain ak； UniProt 1–1935 Chain al； UniProt 1–1935 Chain be； UniProt 1–1935 Chain bf； UniProt 1–1935 Chain bk； UniProt 1–1935 Chain bl； UniProt 1–1935 Chain e； UniProt 1–1935 Chain f； UniProt 1–1935 Chain k； UniProt 1–1935 Chain l； UniProt 1–1935 Not recorded Myosin light chain 3 × 18 （P08590） Myosin regulatory light chain 2, ventricular/cardiac muscle isoform × 18 （P10916） Titin × 6 （Q8WZ42） Myosin-binding protein C, cardiac-type × 3 （Q14896） ELECTRON MICROSCOPY cryo-EM buffer：pH 6.8 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 6.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8G4L Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
2FXM Structure of the human beta-myosin S2 fragment Deposited 2006-02-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 838–963（126 aa） Fragment：DELTA-S2 FRAGMENT (838-963)
Chain B 838–963（126 aa） Fragment：DELTA-S2 FRAGMENT (838-963)
Not recorded HG MERCURY (II) ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；296 K；PEG 3350, LITHIUM CITRATE, TRIS- HCL, pH 8.50, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 296K
Resolution 2.70 Å R-free 0.283
2FXO Structure of the human beta-myosin S2 fragment Deposited 2006-02-06 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 838–963（126 aa） Fragment：DELTA-S2 FRAGMENT (838-963)
Chain B 838–963（126 aa） Fragment：DELTA-S2 FRAGMENT (838-963)
Mutation：E924K Mutation：E924K No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；296 K；PEG 3350, SODIUM ACETATE, TRIS-HCL, pH 7.50, VAPOR DIFFUSION, SITTING DROP, temperature 296K
Resolution 2.50 Å R-free 0.349
2FXO Structure of the human beta-myosin S2 fragment Deposited 2006-02-06 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 838–963（126 aa） Fragment：DELTA-S2 FRAGMENT (838-963)
Chain D 838–963（126 aa） Fragment：DELTA-S2 FRAGMENT (838-963)
Mutation：E924K Mutation：E924K No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；296 K；PEG 3350, SODIUM ACETATE, TRIS-HCL, pH 7.50, VAPOR DIFFUSION, SITTING DROP, temperature 296K
Resolution 2.50 Å R-free 0.349
3DTP Tarantula heavy meromyosin obtained by flexible docking to Tarantula muscle thick filament Cryo-EM 3D-MAP Deposited 2008-07-15 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 216 PDB declaration: 216-meric
Chain A 842–961（120 aa） Fragment：SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-972),SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-972)
Chain B 842–963（122 aa） Fragment：SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-974),SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-974)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 100mM NaCl, 3mM MgCl2, 1mM EGTA, 5mM PIPES, 5mM NaH2PO4, 1mM NaN3；pH 7；100mM NaCl, 3mM MgCl2, 1mM EGTA, 5mM PIPES, 5mM NaH2PO4, 1mM NaN3
cryo-EM vitrification conditions Plunging in a liquid ethane. Blotting was performed from one side of the grid till a thin sample film on it using Whatman No 42 filter paper, then the grid was immediately plunged under gravity into liquid ethane cooled by liquid nitrogen. Grids were stored under liquid nitrogen.
Resolution 20.00 Å
3DTP Tarantula heavy meromyosin obtained by flexible docking to Tarantula muscle thick filament Cryo-EM 3D-MAP Deposited 2008-07-15 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 842–961（120 aa） Fragment：SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-972),SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-972)
Chain B 842–963（122 aa） Fragment：SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-974),SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-974)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 100mM NaCl, 3mM MgCl2, 1mM EGTA, 5mM PIPES, 5mM NaH2PO4, 1mM NaN3；pH 7；100mM NaCl, 3mM MgCl2, 1mM EGTA, 5mM PIPES, 5mM NaH2PO4, 1mM NaN3
cryo-EM vitrification conditions Plunging in a liquid ethane. Blotting was performed from one side of the grid till a thin sample film on it using Whatman No 42 filter paper, then the grid was immediately plunged under gravity into liquid ethane cooled by liquid nitrogen. Grids were stored under liquid nitrogen.
Resolution 20.00 Å
3DTP Tarantula heavy meromyosin obtained by flexible docking to Tarantula muscle thick filament Cryo-EM 3D-MAP Deposited 2008-07-15 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 3 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 842–961（120 aa） Fragment：SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-972),SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-972)
Chain B 842–963（122 aa） Fragment：SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-974),SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-974)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 100mM NaCl, 3mM MgCl2, 1mM EGTA, 5mM PIPES, 5mM NaH2PO4, 1mM NaN3；pH 7；100mM NaCl, 3mM MgCl2, 1mM EGTA, 5mM PIPES, 5mM NaH2PO4, 1mM NaN3
cryo-EM vitrification conditions Plunging in a liquid ethane. Blotting was performed from one side of the grid till a thin sample film on it using Whatman No 42 filter paper, then the grid was immediately plunged under gravity into liquid ethane cooled by liquid nitrogen. Grids were stored under liquid nitrogen.
Resolution 20.00 Å
4DB1 Cardiac human myosin S1dC, beta isoform complexed with Mn-AMPPNP Deposited 2012-01-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 2–783（782 aa） Fragment：UNP residues 2-783
Not recorded ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 MN MANGANESE (II) ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；277 K；precipitant: 7.5% PEG 8000, 0.05M MES, 0.05M acetate, 0.25M sodium chloride, 0.01M manganese chloride, 0.1% sodium cholate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.60 Å R-free 0.257
4DB1 Cardiac human myosin S1dC, beta isoform complexed with Mn-AMPPNP Deposited 2012-01-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 2–783（782 aa） Fragment：UNP residues 2-783
Not recorded ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 MN MANGANESE (II) ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；277 K；precipitant: 7.5% PEG 8000, 0.05M MES, 0.05M acetate, 0.25M sodium chloride, 0.01M manganese chloride, 0.1% sodium cholate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.60 Å R-free 0.257
4DB1 Cardiac human myosin S1dC, beta isoform complexed with Mn-AMPPNP Deposited 2012-01-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 2–783（782 aa） Fragment：UNP residues 2-783
Chain B 2–783（782 aa） Fragment：UNP residues 2-783
Not recorded ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 MN MANGANESE (II) ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；277 K；precipitant: 7.5% PEG 8000, 0.05M MES, 0.05M acetate, 0.25M sodium chloride, 0.01M manganese chloride, 0.1% sodium cholate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.60 Å R-free 0.257
4P7H Structure of Human beta-Cardiac Myosin Motor Domain::GFP chimera Deposited 2014-03-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–787（787 aa） Fragment：UNP P12883 residues 1-787,UNP P42212 residues 5-238
Mutation：Q80R, K101N, V163A, I167T, S175G, D190N Non-standard monomer：Yes (specific site not provided by mmCIF) SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；10% Tacsimate, pH 6.0, 10% glycerol, 14-15% PEG 3350, 0.2 mM MgCL2, and 5 mM TCEP
Resolution 3.20 Å R-free 0.284
4P7H Structure of Human beta-Cardiac Myosin Motor Domain::GFP chimera Deposited 2014-03-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1–787（787 aa） Fragment：UNP P12883 residues 1-787,UNP P42212 residues 5-238
Mutation：Q80R, K101N, V163A, I167T, S175G, D190N Non-standard monomer：Yes (specific site not provided by mmCIF) SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；10% Tacsimate, pH 6.0, 10% glycerol, 14-15% PEG 3350, 0.2 mM MgCL2, and 5 mM TCEP
Resolution 3.20 Å R-free 0.284
4PA0 Omecamtiv Mercarbil binding site on the Human Beta-Cardiac Myosin Motor Domain Deposited 2014-04-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–787（787 aa） Fragment：UNP P12883 residues 1-787, UNP P42212 residues 5-234
Mutation：Q80R, V163A, I167T, S175G, D190N Non-standard monomer：Yes (specific site not provided by mmCIF) 2OW methyl 4-(2-fluoro-3-{[(6-methylpyridin-3-yl)carbamoyl]amino}benzyl)piperazine-1-carboxylate × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；295 K；Tacsimate, pH 6.0, PEG 3350, glycerol, MgCL2, TCEP and ligand, omecamptiv mercarbil
Resolution 2.25 Å R-free 0.246
4PA0 Omecamtiv Mercarbil binding site on the Human Beta-Cardiac Myosin Motor Domain Deposited 2014-04-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1–787（787 aa） Fragment：UNP P12883 residues 1-787, UNP P42212 residues 5-234
Mutation：Q80R, V163A, I167T, S175G, D190N Non-standard monomer：Yes (specific site not provided by mmCIF) 2OW methyl 4-(2-fluoro-3-{[(6-methylpyridin-3-yl)carbamoyl]amino}benzyl)piperazine-1-carboxylate × 1 GOL GLYCEROL × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；295 K；Tacsimate, pH 6.0, PEG 3350, glycerol, MgCL2, TCEP and ligand, omecamptiv mercarbil
Resolution 2.25 Å R-free 0.246
4XA1 Crystal Structure of the coiled-coil surrounding Skip 1 of MYH7 Deposited 2014-12-12 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1173–1238（66 aa） Fragment：UNP P13848 residues 1-49,UNP Q12883 residues 1173-1238,UNP Q15691 residues 211-251
Chain B 1173–1238（66 aa） Fragment：UNP P13848 residues 1-49,UNP Q12883 residues 1173-1238,UNP Q15691 residues 211-251
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.6；298 K；20% (w/v) polyethylene glycol methyl ether 2000, 20 mM SrCl2, 100 mM HEPES pH 7.6, 5% pentaerythritol ethoxylate (17/8 PO/OH) 797, 0.5% 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS)
Resolution 3.20 Å R-free 0.285
4XA1 Crystal Structure of the coiled-coil surrounding Skip 1 of MYH7 Deposited 2014-12-12 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 1173–1238（66 aa） Fragment：UNP P13848 residues 1-49,UNP Q12883 residues 1173-1238,UNP Q15691 residues 211-251
Chain D 1173–1238（66 aa） Fragment：UNP P13848 residues 1-49,UNP Q12883 residues 1173-1238,UNP Q15691 residues 211-251
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.6；298 K；20% (w/v) polyethylene glycol methyl ether 2000, 20 mM SrCl2, 100 mM HEPES pH 7.6, 5% pentaerythritol ethoxylate (17/8 PO/OH) 797, 0.5% 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS)
Resolution 3.20 Å R-free 0.285
4XA3 Crystal structure of the coiled-coil surrounding Skip 2 of MYH7 Deposited 2014-12-12 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1361–1425（65 aa） Fragment：UNP P13848 residues 1-49,UNP P12883 residues 1361-1425,UNP Q15691 residues 215-251
Chain B 1361–1425（65 aa） Fragment：UNP P13848 residues 1-49,UNP P12883 residues 1361-1425,UNP Q15691 residues 215-251
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5；298 K；4.5% (w/v) polyethylene glycol 8000, 100 mM sodium acetate pH 5.0, 50 mM CaCl2, 2.5% (w/v) 3-methoxy-3-methyl-1-butanol
Resolution 2.55 Å R-free 0.311
4XA4 Crystal Structure of the coiled-coil surrounding Skip 3 of MYH7 Deposited 2014-12-12 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1551–1609（59 aa） Fragment：UNP Q13426 residues 2-147,UNP P12883 residues 1551-1609
Chain B 1551–1609（59 aa） Fragment：UNP Q13426 residues 2-147,UNP P12883 residues 1551-1609
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；298 K；30% (w/v) polyethylene glycol 1500, 250 mM tetramethylammonium chloride, 100 mM 3-[4-(2-Hydroxyethyl)-1-piperazinyl]propanesulfonic acid (HEPPS)
Resolution 2.33 Å R-free 0.274
4XA6 Crystal Structure of the coiled-coil surrounding Skip 4 of MYH7 Deposited 2014-12-12 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1777–1855（79 aa） Fragment：UNP P13848 residues 2-50,UNP P02564 residues 1777-1855,UNP Q15691 residues 209-251
Chain B 1777–1855（79 aa） Fragment：UNP P13848 residues 2-50,UNP P02564 residues 1777-1855,UNP Q15691 residues 209-251
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.4；298 K；18% (w/v) polyethylene glycol 2000 methyl ether, 100 mM piperazine-N,N-bis(2-ethanesulfonic acid) (PIPES)
Resolution 3.42 Å R-free 0.297
4XA6 Crystal Structure of the coiled-coil surrounding Skip 4 of MYH7 Deposited 2014-12-12 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 1777–1855（79 aa） Fragment：UNP P13848 residues 2-50,UNP P02564 residues 1777-1855,UNP Q15691 residues 209-251
Chain D 1777–1855（79 aa） Fragment：UNP P13848 residues 2-50,UNP P02564 residues 1777-1855,UNP Q15691 residues 209-251
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.4；298 K；18% (w/v) polyethylene glycol 2000 methyl ether, 100 mM piperazine-N,N-bis(2-ethanesulfonic acid) (PIPES)
Resolution 3.42 Å R-free 0.297
5CHX Crystal Structure of amino acids 1590-1657 of MYH7 Deposited 2015-07-10 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1590–1657（68 aa） Fragment：UNP Q13426 residues 2-143, UNP P12833 1590-1657
Chain B 1590–1657（68 aa） Fragment：UNP Q13426 residues 2-143, UNP P12833 1590-1657
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；16% (w/v) MEPEG 2000, 250 mM potassium nitrate, 100 mM 3-(N-morpholino)propanesulfonic acid (MOPS)
Resolution 2.30 Å R-free 0.265
5CHX Crystal Structure of amino acids 1590-1657 of MYH7 Deposited 2015-07-10 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1590–1657（68 aa） Fragment：UNP Q13426 residues 2-143, UNP P12833 1590-1657
Chain B 1590–1657（68 aa） Fragment：UNP Q13426 residues 2-143, UNP P12833 1590-1657
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；16% (w/v) MEPEG 2000, 250 mM potassium nitrate, 100 mM 3-(N-morpholino)propanesulfonic acid (MOPS)
Resolution 2.30 Å R-free 0.265
5CJ0 Crystal Structure of Amino Acids 1631-1692 of MYH7 Deposited 2015-07-13 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1631–1692（62 aa） Fragment：UNP Q13426 residues 2-142, UNP P12883 residues 1631-1692
Chain B 1631–1692（62 aa） Fragment：UNP Q13426 residues 2-142, UNP P12883 residues 1631-1692
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；14% (w/v) MEPEG 5000, 200 mM glycine, 100 mM bistrispropane pH 7.0.
Resolution 2.30 Å R-free 0.238
5CJ1 Crystal structure of the coiled coil of MYH7 residues 1526 to 1571 fused to Gp7 Deposited 2015-07-13 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1526–1571（46 aa） Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571
Chain B 1526–1571（46 aa） Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；16% (w/v) PEG 8000, 400 mM malonate pH 7.2, and 100 mM triethanolamine pH 7.5
Resolution 2.10 Å R-free 0.250
5CJ1 Crystal structure of the coiled coil of MYH7 residues 1526 to 1571 fused to Gp7 Deposited 2015-07-13 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 1526–1571（46 aa） Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571
Chain D 1526–1571（46 aa） Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；16% (w/v) PEG 8000, 400 mM malonate pH 7.2, and 100 mM triethanolamine pH 7.5
Resolution 2.10 Å R-free 0.250
5CJ1 Crystal structure of the coiled coil of MYH7 residues 1526 to 1571 fused to Gp7 Deposited 2015-07-13 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain E 1526–1571（46 aa） Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571
Chain F 1526–1571（46 aa） Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；16% (w/v) PEG 8000, 400 mM malonate pH 7.2, and 100 mM triethanolamine pH 7.5
Resolution 2.10 Å R-free 0.250
5CJ1 Crystal structure of the coiled coil of MYH7 residues 1526 to 1571 fused to Gp7 Deposited 2015-07-13 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain G 1526–1571（46 aa） Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571
Chain H 1526–1571（46 aa） Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；16% (w/v) PEG 8000, 400 mM malonate pH 7.2, and 100 mM triethanolamine pH 7.5
Resolution 2.10 Å R-free 0.250
5CJ4 Crystal Structure of Amino Acids 1562-1622 of MYH7 Deposited 2015-07-13 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1562–1622（61 aa） Fragment：UNP Q13426 residues 2-144, UNP P12883 1562-1622
Chain B 1562–1622（61 aa） Fragment：UNP Q13426 residues 2-144, UNP P12883 1562-1622
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；23% (w/v) PEG 4000, 500 mM NaCl, 100 mM triethanolamine pH 8.0.
Resolution 3.10 Å R-free 0.280
5CJ4 Crystal Structure of Amino Acids 1562-1622 of MYH7 Deposited 2015-07-13 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 1562–1622（61 aa） Fragment：UNP Q13426 residues 2-144, UNP P12883 1562-1622
Chain D 1562–1622（61 aa） Fragment：UNP Q13426 residues 2-144, UNP P12883 1562-1622
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；23% (w/v) PEG 4000, 500 mM NaCl, 100 mM triethanolamine pH 8.0.
Resolution 3.10 Å R-free 0.280
5TBY HUMAN BETA CARDIAC HEAVY MEROMYOSIN INTERACTING-HEADS MOTIF OBTAINED BY HOMOLOGY MODELING (USING SWISS-MODEL) OF HUMAN SEQUENCE FROM APHONOPELMA HOMOLOGY MODEL (PDB-3JBH), RIGIDLY FITTED TO HUMAN BETA-CARDIAC NEGATIVELY STAINED THICK FILAMENT 3D-RECONSTRUCTION (EMD-2240) Deposited 2016-09-13 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–1935（1935 aa） Fragment：SUBFRAGMENT 1(S1)
Chain B 1–1935（1935 aa） Fragment：SUBFRAGMENT 1(S1)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE；PLUNGING IN A LIQUID ETHANE COOLED BY LIQUID NITROGEN. BLOTTING WAS PERFORMED FROM ONE SIDE OF THE GRID TILL A THIN SAMPLE FILM ON IT USING WHATMAN NO. 42 FILTER PAPER, THEN THE GRID WAS IMMEDIATELY PLUNGED UNDER GRAVITY INTO LIQUID ETHANE COOLED BY LIQUID NITROGEN. GRIDS WERE STORED UNDER LIQUID NITROGEN.
Resolution 20.00 Å
5WJ7 Crystal Structure of Amino Acids 1733-1797 of Human Beta Cardiac Myosin Fused to Xrcc4 Deposited 2017-07-21 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1733–1797（65 aa） Fragment：UNP Q13426 residues 2-132, UNP P12883 residues 1733-1797
Chain B 1733–1797（65 aa） Fragment：UNP Q13426 residues 2-132, UNP P12883 residues 1733-1797
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；16% (w/v) Methyl-Ether PEG 5K, 300 mM glycine, 100 mM triethanolamine pH 7.5
Resolution 2.50 Å R-free 0.249
5WJB Crystal Structure of Amino Acids 1733-1797 of Human Beta Cardiac Myosin Fused to Gp7 Deposited 2017-07-21 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1733–1797（65 aa） Fragment：UNP P13848 residues 2-47, UNP P12833 residues 1733-1797
Chain B 1733–1797（65 aa） Fragment：UNP P13848 residues 2-47, UNP P12833 residues 1733-1797
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；14% (w/v) methyl-ether PEG 2K, 1.5%(w/v) myo-inositol, 100 mM HEPES pH 7.5, 50 mM magnesium chloride
Resolution 2.90 Å R-free 0.300
5WJB Crystal Structure of Amino Acids 1733-1797 of Human Beta Cardiac Myosin Fused to Gp7 Deposited 2017-07-21 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 1733–1797（65 aa） Fragment：UNP P13848 residues 2-47, UNP P12833 residues 1733-1797
Chain D 1733–1797（65 aa） Fragment：UNP P13848 residues 2-47, UNP P12833 residues 1733-1797
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；14% (w/v) methyl-ether PEG 2K, 1.5%(w/v) myo-inositol, 100 mM HEPES pH 7.5, 50 mM magnesium chloride
Resolution 2.90 Å R-free 0.300
5WLQ Crystal Structure of Amino Acids 1677-1755 of Human Beta Cardiac Myosin Fused to Gp7 and Eb1 Deposited 2017-07-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1677–1755（79 aa） Fragment：UNP P13848 residues 2-48 UNP Q15691 residues 208-256, UNP P12883 residues 1677-1755
Not recorded SO4 SULFATE ION × 2 TMO trimethylamine oxide × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；298 K；1.6 M Ammonium Aulfate, 500 mM Trimethyl Ammonium N-Oxide, 100 mM Bis-tris Propane pH 9.0
Resolution 3.10 Å R-free 0.236
5WLZ Crystal Structure of Amino Acids 1677-1758 of Human Beta Cardiac Myosin Fused to Xrcc4 Deposited 2017-07-28 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1677–1758（82 aa） Fragment：UNP Q13426 residues 2-132, UNP P12883 residues 1677-1758
Chain B 1677–1758（82 aa） Fragment：UNP Q13426 residues 2-132, UNP P12883 residues 1677-1758
Chain C 1677–1758（82 aa） Fragment：UNP Q13426 residues 2-132, UNP P12883 residues 1677-1758
Chain D 1677–1758（82 aa） Fragment：UNP Q13426 residues 2-132, UNP P12883 residues 1677-1758
Mutation：E29K, E51K, D57A, D58T, E62N, C93R, E98K,C128D, C132A Mutation：E29K, E51K, D57A, D58T, E62N, C93R, E98K,C128D, C132A Mutation：E29K, E51K, D57A, D58T, E62N, C93R, E98K,C128D, C132A Mutation：E29K, E51K, D57A, D58T, E62N, C93R, E98K,C128D, C132A No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；8-10% methyl-ether polyethylene glycol (MEPEG) 5K, 300 mM glycine, bis-tris propane pH 7.0, 1.5-3.0% (w/v) jeffamine M-600
Resolution 3.50 Å R-free 0.249
5WME Crystal Structure of Amino Acids 1729-1786 of Human Beta Cardiac Myosin Fused to Gp7 as Anti-Parallel Four-Helix Bundle Deposited 2017-07-28 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1728–1786（59 aa） Fragment：UNP P13848 residues 2-48, UNP P12883 residues 1729-1786
Chain B 1728–1786（59 aa） Fragment：UNP P13848 residues 2-48, UNP P12883 residues 1729-1786
Chain C 1728–1786（59 aa） Fragment：UNP P13848 residues 2-48, UNP P12883 residues 1729-1786
Chain D 1728–1786（59 aa） Fragment：UNP P13848 residues 2-48, UNP P12883 residues 1729-1786
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5；298 K；18% (w/v) pentaerythritol ethoxylate 797, 150 mM ammonium thiocyanate, 100 mM sodium acetate pH 5.0.
Resolution 2.30 Å R-free 0.264
8ACT structure of the human beta-cardiac myosin folded-back off state Deposited 2022-07-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 3–906（904 aa）
Chain B 3–906（904 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) ADP ADENOSINE-5'-DIPHOSPHATE × 2 PO4 PHOSPHATE ION × 2 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8EFD Human cardiac myosin II and associated essential light chain in the rigor conformation Deposited 2022-09-08 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–842（842 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8EFE Human beta-cardiac myosin II bound to ADP-MG2+ and the associated essential light chain Deposited 2022-09-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–842（842 aa）
Not recorded MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8EFH Helical reconstruction of the human cardiac actin-tropomyosin-myosin complex in complex with ADP-Mg2+ Deposited 2022-09-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–842（842 aa）
Not recorded MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8EFI Helical reconstruction of the human cardiac actin-tropomyosin-myosin complex in the rigor form Deposited 2022-09-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain M 1–1935（1935 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8ENC Helical reconstruction of the human cardiac actin-tropomyosin-myosin loop 4 7G mutant complex Deposited 2022-09-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain M 1–1935（1935 aa）
Mutation：Residues 366-372 substituted with seven glycines ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8ZB7 Human left ventricle ATM complex Deposited 2024-04-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain G 6–781（776 aa）
Chain H 6–781（776 aa）
Chain I 6–781（776 aa）
Chain J 6–781（776 aa）
Chain K 6–781（776 aa）
Chain M 6–781（776 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.19 Å
8ZI9 Human left ventricle actin and myosin complex Deposited 2024-05-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain M 6–781（776 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.08 Å
9GZ1 Beta-cardiac myosin interacting heads motif complexed to mavacamten Deposited 2024-10-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 2–1138（1137 aa）
Chain B 2–1138（1137 aa）
Not recorded MG MAGNESIUM ION × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 2 PO4 PHOSPHATE ION × 2 XB2 Mavacamten × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.2
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
9GZ2 Beta-cardiac heavy meromyosin motor domain in the primed state complexed to mavacamten Deposited 2024-10-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 2–1138（1137 aa）
Not recorded MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1 XB2 Mavacamten × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.2
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9GZ3 Beta-cardiac heavy meromyosin motor domain in the primed state Deposited 2024-10-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 2–1138（1137 aa）
Not recorded MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.2
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
9HTF Beta-cardiac myosin Y115H mutant motor domain in the pre-powerstroke state, MgADP.VO4 form Deposited 2024-12-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–810（810 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 VO4 VANADATE ION × 1 GOL GLYCEROL × 1 EDO 1,2-ETHANEDIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；23 % PEG 3350 w:v, 0.3 M lithium sulfate, 0.1M Tris-HCl, 2 mM Mg.ADP.Vanadate
Resolution 2.48 Å R-free 0.255
9HTG Beta-cardiac myosin E497D mutant motor domain in the pre-powerstroke state, MgADP.VO4 form Deposited 2024-12-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–808（808 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 VO4 VANADATE ION × 1 GOL GLYCEROL × 1 EDO 1,2-ETHANEDIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；22 % PEG 3350 w:v, 0.3 M lithium sulfate, 0.1M Tris-HCl, 2 mM Mg.ADP.Vanadate
Resolution 2.60 Å R-free 0.248
9I8P Human beta-cardiac myosin wild type motor domain in the pre-powerstroke state, MgADP.VO4 form Deposited 2025-02-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–808（808 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 VO4 VANADATE ION × 1 EDO 1,2-ETHANEDIOL × 2 SO4 SULFATE ION × 2 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；25 % PEG 3350 w:v, 0.25 M lithium sulfate, 0.1M Tris-HCl, 2 mM Mg.ADP.Vanadate. Optimal crystals were obtained using the micro-seeding technique.
Resolution 2.60 Å R-free 0.245
9YOP Cryo-EM structure of human beta-cardiac myosin in the interacting-heads motif and S2-FH docked state Deposited 2025-10-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–1016（1016 aa）
Chain B 1–1016（1016 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 2 PO4 PHOSPHATE ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
9YP4 Cryo-EM structure of human beta-cardiac myosin bound to omecamtiv mecarbil in the interacting-heads motif and S2-FH docked state Deposited 2025-10-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–1016（1016 aa）
Chain B 1–1016（1016 aa）
Not recorded 2OW methyl 4-(2-fluoro-3-{[(6-methylpyridin-3-yl)carbamoyl]amino}benzyl)piperazine-1-carboxylate × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 PO4 PHOSPHATE ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.50 Å
9YP9 Cryo-EM structure of human beta-cardiac myosin bound to mavacamten in the interacting-heads motif and S2-FH docked state Deposited 2025-10-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–1016（1016 aa）
Chain B 1–1016（1016 aa）
Not recorded PO4 PHOSPHATE ION × 2 XB2 Mavacamten × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YR7 Cryo-EM structure of human beta-cardiac myosin bound to mavacamten in the interacting-heads motif and S2-FH undocked state Deposited 2025-10-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–1016（1016 aa）
Chain B 1–1016（1016 aa）
Not recorded XB2 Mavacamten × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 PO4 PHOSPHATE ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YRG Cryo-EM structure of human beta-cardiac myosin in the interacting-heads motif and S2-FH undocked state Deposited 2025-10-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–1016（1016 aa）
Chain B 1–1016（1016 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 2 PO4 PHOSPHATE ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
9YRH Cryo-EM structure of human beta-cardiac myosin bound to omecamtiv mecarbil in the interacting-heads motif and S2-FH undocked state Deposited 2025-10-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–1016（1016 aa）
Chain B 1–1016（1016 aa）
Not recorded 2OW methyl 4-(2-fluoro-3-{[(6-methylpyridin-3-yl)carbamoyl]amino}benzyl)piperazine-1-carboxylate × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 PO4 PHOSPHATE ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å

40 other PDB entries and 55 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name MYH7_HUMAN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–1935； UniProt 1–1935 Author chain AA； PDBConstruct 1–1935； UniProt 1–1935 Author chain AB； PDBConstruct 1–1935； UniProt 1–1935 Author chain AG； PDBConstruct 1–1935； UniProt 1–1935 Author chain AH； PDBConstruct 1–1935； UniProt 1–1935 Author chain AI； PDBConstruct 1–1935； UniProt 1–1935 Author chain AJ； PDBConstruct 1–1935； UniProt 1–1935 Author chain AK； PDBConstruct 1–1935； UniProt 1–1935 Author chain AL； PDBConstruct 1–1935； UniProt 1–1935 Author chain AM； PDBConstruct 1–1935； UniProt 1–1935 Author chain AN； PDBConstruct 1–1935； UniProt 1–1935 Author chain AO； PDBConstruct 1–1935； UniProt 1–1935 Author chain AP； PDBConstruct 1–1935； UniProt 1–1935 Author chain AQ； PDBConstruct 1–1935； UniProt 1–1935 Author chain AR； PDBConstruct 1–1935； UniProt 1–1935 Author chain AS； PDBConstruct 1–1935； UniProt 1–1935 Author chain AT； PDBConstruct 1–1935； UniProt 1–1935 Author chain AU； PDBConstruct 1–1935； UniProt 1–1935 Author chain AV； PDBConstruct 1–1935； UniProt 1–1935 Author chain AW； PDBConstruct 1–1935； UniProt 1–1935 Author chain AX； PDBConstruct 1–1935； UniProt 1–1935 Author chain AY； PDBConstruct 1–1935； UniProt 1–1935 Author chain AZ； PDBConstruct 1–1935； UniProt 1–1935 Author chain B； PDBConstruct 1–1935； UniProt 1–1935 Author chain BA； PDBConstruct 1–1935； UniProt 1–1935 Author chain BB； PDBConstruct 1–1935； UniProt 1–1935 Author chain BG； PDBConstruct 1–1935； UniProt 1–1935 Author chain BH； PDBConstruct 1–1935； UniProt 1–1935 Author chain BI； PDBConstruct 1–1935； UniProt 1–1935 Author chain BJ； PDBConstruct 1–1935； UniProt 1–1935 Author chain BK； PDBConstruct 1–1935； UniProt 1–1935 Author chain BL； PDBConstruct 1–1935； UniProt 1–1935 Author chain BM； PDBConstruct 1–1935； UniProt 1–1935 Author chain BN； PDBConstruct 1–1935； UniProt 1–1935 Author chain BO； PDBConstruct 1–1935； UniProt 1–1935 Author chain BP； PDBConstruct 1–1935； UniProt 1–1935 Author chain BQ； PDBConstruct 1–1935； UniProt 1–1935 Author chain BR； PDBConstruct 1–1935； UniProt 1–1935 Author chain BS； PDBConstruct 1–1935； UniProt 1–1935 Author chain BT； PDBConstruct 1–1935； UniProt 1–1935 Author chain BU； PDBConstruct 1–1935； UniProt 1–1935 Author chain BV； PDBConstruct 1–1935； UniProt 1–1935 Author chain BW； PDBConstruct 1–1935； UniProt 1–1935 Author chain BX； PDBConstruct 1–1935； UniProt 1–1935 Author chain BY； PDBConstruct 1–1935； UniProt 1–1935 Author chain BZ； PDBConstruct 1–1935； UniProt 1–1935 Author chain G； PDBConstruct 1–1935； UniProt 1–1935 Author chain H； PDBConstruct 1–1935； UniProt 1–1935 Author chain I； PDBConstruct 1–1935； UniProt 1–1935 Author chain J； PDBConstruct 1–1935； UniProt 1–1935 Author chain K； PDBConstruct 1–1935； UniProt 1–1935 Author chain L； PDBConstruct 1–1935； UniProt 1–1935 Author chain M； PDBConstruct 1–1935； UniProt 1–1935 Author chain N； PDBConstruct 1–1935； UniProt 1–1935 Author chain O； PDBConstruct 1–1935； UniProt 1–1935 Author chain P； PDBConstruct 1–1935； UniProt 1–1935 Author chain Q； PDBConstruct 1–1935； UniProt 1–1935 Author chain R； PDBConstruct 1–1935； UniProt 1–1935 Author chain S； PDBConstruct 1–1935； UniProt 1–1935 Author chain T； PDBConstruct 1–1935； UniProt 1–1935 Author chain U； PDBConstruct 1–1935； UniProt 1–1935 Author chain V； PDBConstruct 1–1935； UniProt 1–1935 Author chain W； PDBConstruct 1–1935； UniProt 1–1935 Author chain X； PDBConstruct 1–1935； UniProt 1–1935 Author chain Y； PDBConstruct 1–1935； UniProt 1–1935 Author chain Z； PDBConstruct 1–1935； UniProt 1–1935 Author chain ae； PDBConstruct 1–1935； UniProt 1–1935 Author chain af； PDBConstruct 1–1935； UniProt 1–1935 Author chain ak； PDBConstruct 1–1935； UniProt 1–1935 Author chain al； PDBConstruct 1–1935； UniProt 1–1935 Author chain be； PDBConstruct 1–1935； UniProt 1–1935 Author chain bf； PDBConstruct 1–1935； UniProt 1–1935 Author chain bk； PDBConstruct 1–1935； UniProt 1–1935 Author chain bl； PDBConstruct 1–1935； UniProt 1–1935 Author chain e； PDBConstruct 1–1935； UniProt 1–1935 Author chain f； PDBConstruct 1–1935； UniProt 1–1935 Author chain k； PDBConstruct 1–1935； UniProt 1–1935 Author chain l； PDBConstruct 1–1935； UniProt 1–1935

Myosin light chain 3

OrganismNot specified

UniProt P08590

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 123 PDB declaration: 123-meric（123） Consistent with protein copy count Chain AE； UniProt 1–195 Chain AF； UniProt 1–195 Chain BE； UniProt 1–195 Chain BF； UniProt 1–195 Chain E； UniProt 1–195 Chain F； UniProt 1–195 Chain a； UniProt 1–195 Chain aa； UniProt 1–195 Chain ab； UniProt 1–195 Chain ag； UniProt 1–195 Chain ah； UniProt 1–195 Chain b； UniProt 1–195 Chain ba； UniProt 1–195 Chain bb； UniProt 1–195 Chain bg； UniProt 1–195 Chain bh； UniProt 1–195 Chain g； UniProt 1–195 Chain h； UniProt 1–195 Not recorded Myosin-7 × 78 （P12883） Myosin regulatory light chain 2, ventricular/cardiac muscle isoform × 18 （P10916） Titin × 6 （Q8WZ42） Myosin-binding protein C, cardiac-type × 3 （Q14896） ELECTRON MICROSCOPY cryo-EM buffer：pH 6.8 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 6.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8G4L Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5TBY HUMAN BETA CARDIAC HEAVY MEROMYOSIN INTERACTING-HEADS MOTIF OBTAINED BY HOMOLOGY MODELING (USING SWISS-MODEL) OF HUMAN SEQUENCE FROM APHONOPELMA HOMOLOGY MODEL (PDB-3JBH), RIGIDLY FITTED TO HUMAN BETA-CARDIAC NEGATIVELY STAINED THICK FILAMENT 3D-RECONSTRUCTION (EMD-2240) Deposited 2016-09-13 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain C 1–195（195 aa）
Chain D 1–195（195 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE；PLUNGING IN A LIQUID ETHANE COOLED BY LIQUID NITROGEN. BLOTTING WAS PERFORMED FROM ONE SIDE OF THE GRID TILL A THIN SAMPLE FILM ON IT USING WHATMAN NO. 42 FILTER PAPER, THEN THE GRID WAS IMMEDIATELY PLUNGED UNDER GRAVITY INTO LIQUID ETHANE COOLED BY LIQUID NITROGEN. GRIDS WERE STORED UNDER LIQUID NITROGEN.
Resolution 20.00 Å
8ACT structure of the human beta-cardiac myosin folded-back off state Deposited 2022-07-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain C 39–195（157 aa）
Chain D 39–195（157 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 2 PO4 PHOSPHATE ION × 2 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name MYL3_HUMAN
Isoform —
PDB entities 2
Chains and sequence ranges Author chain AE； PDBConstruct 1–195； UniProt 1–195 Author chain AF； PDBConstruct 1–195； UniProt 1–195 Author chain BE； PDBConstruct 1–195； UniProt 1–195 Author chain BF； PDBConstruct 1–195； UniProt 1–195 Author chain E； PDBConstruct 1–195； UniProt 1–195 Author chain F； PDBConstruct 1–195； UniProt 1–195 Author chain a； PDBConstruct 1–195； UniProt 1–195 Author chain aa； PDBConstruct 1–195； UniProt 1–195 Author chain ab； PDBConstruct 1–195； UniProt 1–195 Author chain ag； PDBConstruct 1–195； UniProt 1–195 Author chain ah； PDBConstruct 1–195； UniProt 1–195 Author chain b； PDBConstruct 1–195； UniProt 1–195 Author chain ba； PDBConstruct 1–195； UniProt 1–195 Author chain bb； PDBConstruct 1–195； UniProt 1–195 Author chain bg； PDBConstruct 1–195； UniProt 1–195 Author chain bh； PDBConstruct 1–195； UniProt 1–195 Author chain g； PDBConstruct 1–195； UniProt 1–195 Author chain h； PDBConstruct 1–195； UniProt 1–195

Myosin regulatory light chain 2, ventricular/cardiac muscle isoform

OrganismNot specified

UniProt P10916

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 123 PDB declaration: 123-meric（123） Consistent with protein copy count Chain ac； UniProt 1–166 Chain ad； UniProt 1–166 Chain ai； UniProt 1–166 Chain aj； UniProt 1–166 Chain aq； UniProt 1–166 Chain ar； UniProt 1–166 Chain bc； UniProt 1–166 Chain bd； UniProt 1–166 Chain bi； UniProt 1–166 Chain bj； UniProt 1–166 Chain bq； UniProt 1–166 Chain br； UniProt 1–166 Chain c； UniProt 1–166 Chain d； UniProt 1–166 Chain i； UniProt 1–166 Chain j； UniProt 1–166 Chain q； UniProt 1–166 Chain r； UniProt 1–166 Not recorded Myosin-7 × 78 （P12883） Myosin light chain 3 × 18 （P08590） Titin × 6 （Q8WZ42） Myosin-binding protein C, cardiac-type × 3 （Q14896） ELECTRON MICROSCOPY cryo-EM buffer：pH 6.8 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 6.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8G4L Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5TBY HUMAN BETA CARDIAC HEAVY MEROMYOSIN INTERACTING-HEADS MOTIF OBTAINED BY HOMOLOGY MODELING (USING SWISS-MODEL) OF HUMAN SEQUENCE FROM APHONOPELMA HOMOLOGY MODEL (PDB-3JBH), RIGIDLY FITTED TO HUMAN BETA-CARDIAC NEGATIVELY STAINED THICK FILAMENT 3D-RECONSTRUCTION (EMD-2240) Deposited 2016-09-13 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain E 1–166（166 aa）
Chain F 1–166（166 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE；PLUNGING IN A LIQUID ETHANE COOLED BY LIQUID NITROGEN. BLOTTING WAS PERFORMED FROM ONE SIDE OF THE GRID TILL A THIN SAMPLE FILM ON IT USING WHATMAN NO. 42 FILTER PAPER, THEN THE GRID WAS IMMEDIATELY PLUNGED UNDER GRAVITY INTO LIQUID ETHANE COOLED BY LIQUID NITROGEN. GRIDS WERE STORED UNDER LIQUID NITROGEN.
Resolution 20.00 Å
8ACT structure of the human beta-cardiac myosin folded-back off state Deposited 2022-07-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain E 20–163（144 aa）
Chain F 20–163（144 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 2 PO4 PHOSPHATE ION × 2 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name MLRV_HUMAN
Isoform —
PDB entities 3
Chains and sequence ranges Author chain ac； PDBConstruct 1–166； UniProt 1–166 Author chain ad； PDBConstruct 1–166； UniProt 1–166 Author chain ai； PDBConstruct 1–166； UniProt 1–166 Author chain aj； PDBConstruct 1–166； UniProt 1–166 Author chain aq； PDBConstruct 1–166； UniProt 1–166 Author chain ar； PDBConstruct 1–166； UniProt 1–166 Author chain bc； PDBConstruct 1–166； UniProt 1–166 Author chain bd； PDBConstruct 1–166； UniProt 1–166 Author chain bi； PDBConstruct 1–166； UniProt 1–166 Author chain bj； PDBConstruct 1–166； UniProt 1–166 Author chain bq； PDBConstruct 1–166； UniProt 1–166 Author chain br； PDBConstruct 1–166； UniProt 1–166 Author chain c； PDBConstruct 1–166； UniProt 1–166 Author chain d； PDBConstruct 1–166； UniProt 1–166 Author chain i； PDBConstruct 1–166； UniProt 1–166 Author chain j； PDBConstruct 1–166； UniProt 1–166 Author chain q； PDBConstruct 1–166； UniProt 1–166 Author chain r； PDBConstruct 1–166； UniProt 1–166

Titin

OrganismNot specified

UniProt Q8WZ42

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 123 PDB declaration: 123-meric（123） Consistent with protein copy count Chain am； UniProt 22784–23867 Chain an； UniProt 22784–23867 Chain bm； UniProt 22784–23867 Chain bn； UniProt 22784–23867 Chain m； UniProt 22784–23867 Chain n； UniProt 22784–23867 Not recorded Myosin-7 × 78 （P12883） Myosin light chain 3 × 18 （P08590） Myosin regulatory light chain 2, ventricular/cardiac muscle isoform × 18 （P10916） Myosin-binding protein C, cardiac-type × 3 （Q14896） ELECTRON MICROSCOPY cryo-EM buffer：pH 6.8 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 6.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8G4L Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1TNM TERTIARY STRUCTURE OF AN IMMUNOGLOBULIN-LIKE DOMAIN FROM THE GIANT MUSCLE PROTEIN TITIN: A NEW MEMBER OF THE I SET Deposited 1995-01-17 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 33489–33579（91 aa）
Not recorded No recorded non-water small molecule SOLUTION NMR mmCIF provides none of the parsed conditions Resolution not provided
1TNN Tertiary structure of an immunoglobulin-like domain from the giant muscle protein titin: a new member of the I set Deposited 1995-01-17 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 33489–33579（91 aa）
Not recorded No recorded non-water small molecule SOLUTION NMR mmCIF provides none of the parsed conditions Resolution not provided
1WAA IG27 protein domain Deposited 2004-10-25 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain A 12801–12889（89 aa） Fragment：IG DOMAIN, RESIDUES 12801-12889
Chain B 12801–12889（89 aa） Fragment：IG DOMAIN, RESIDUES 12801-12889
Chain C 12801–12889（89 aa） Fragment：IG DOMAIN, RESIDUES 12801-12889
Chain D 12801–12889（89 aa） Fragment：IG DOMAIN, RESIDUES 12801-12889
Chain E 12801–12889（89 aa） Fragment：IG DOMAIN, RESIDUES 12801-12889
Chain F 12801–12889（89 aa） Fragment：IG DOMAIN, RESIDUES 12801-12889
Not recorded ZN ZINC ION × 24 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.80 Å R-free 0.268
2A38 Crystal structure of the N-Terminus of titin Deposited 2005-06-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–194（194 aa） Fragment：Ig-like 1/2, residues 1-194
Not recorded CD CADMIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；MES, Cadmium sulphate, Sodium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.00 Å R-free 0.255
2A38 Crystal structure of the N-Terminus of titin Deposited 2005-06-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1–194（194 aa） Fragment：Ig-like 1/2, residues 1-194
Not recorded CD CADMIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；MES, Cadmium sulphate, Sodium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.00 Å R-free 0.255
2A38 Crystal structure of the N-Terminus of titin Deposited 2005-06-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 1–194（194 aa） Fragment：Ig-like 1/2, residues 1-194
Not recorded CD CADMIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；MES, Cadmium sulphate, Sodium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.00 Å R-free 0.255
2BK8 M1 domain from titin Deposited 2005-02-11 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 32497–32590（94 aa） Fragment：RESIDUES 25073-25166 (IG-LIKE DOMAIN)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；2.2 M AMMONIUM SULFATE 0.1 M BIS TRIS PH 7.0
Resolution 1.69 Å R-free 0.234
2F8V Structure of full length telethonin in complex with the N-terminus of titin Deposited 2005-12-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–196（196 aa） Fragment：Domains Z1Z2, residues 1-196
Chain B 1–196（196 aa） Fragment：Domains Z1Z2, residues 1-196
Not recorded SO4 SULFATE ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.45；298 K；PEG35000, LITHIUM SULPHATE, ACETATE BUFFER, pH 4.45, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.75 Å R-free 0.287
2F8V Structure of full length telethonin in complex with the N-terminus of titin Deposited 2005-12-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 1–196（196 aa） Fragment：Domains Z1Z2, residues 1-196
Chain D 1–196（196 aa） Fragment：Domains Z1Z2, residues 1-196
Not recorded SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.45；298 K；PEG35000, LITHIUM SULPHATE, ACETATE BUFFER, pH 4.45, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.75 Å R-free 0.287
2ILL Anomalous substructure of Titin-A168169 Deposited 2006-10-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 31854–32047（194 aa） Fragment：Titin-A168169
Not recorded CL CHLORIDE ION × 2 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.20 Å R-free 0.243
2J8H Structure of the immunoglobulin tandem repeat A168-A169 of titin Deposited 2006-10-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 24430–24623（194 aa） Fragment：IG LIKE DOMAIN, RESIDUES 24430-24623
Not recorded GOL GLYCEROL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 9；AMMONIUM SULFATE, BICINE, pH 9
Resolution 1.99 Å R-free 0.210
2J8O Structure of the immunoglobulin tandem repeat of titin A168-A169 Deposited 2006-10-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 24430–24623（194 aa） Fragment：IG LIKE DOMAIN, RESIDUES 24430-24623
Not recorded GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 9；AMMONIUM SULFATE, BICINE, pH 9
Resolution 2.49 Å R-free 0.300
2J8O Structure of the immunoglobulin tandem repeat of titin A168-A169 Deposited 2006-10-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 24430–24623（194 aa） Fragment：IG LIKE DOMAIN, RESIDUES 24430-24623
Not recorded GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 9；AMMONIUM SULFATE, BICINE, pH 9
Resolution 2.49 Å R-free 0.300
2NZI Crystal structure of domains A168-A170 from titin Deposited 2006-11-23 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 31854–32155（302 aa） Fragment：Residues 31854-32155
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；298 K；2.1M NaCl, 0.1M MES, 0.6M Li2SO4, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.90 Å R-free 0.274
2NZI Crystal structure of domains A168-A170 from titin Deposited 2006-11-23 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 31854–32155（302 aa） Fragment：Residues 31854-32155
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；298 K；2.1M NaCl, 0.1M MES, 0.6M Li2SO4, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.90 Å R-free 0.274
2RQ8 Solution NMR structure of titin I27 domain mutant Deposited 2009-03-05 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 12677–12765（89 aa） Fragment：UNP residues 12677-12765
Mutation：K3E, Y9P, T42A, A78T No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 7.4；298 K；Ionic strength (raw mmCIF value) 50mM POTASSIUM PHOSPHATE；Pressure 1
NMR sample composition 1.0mM TITIN I27 mutant, 50mM potassium phosphate buffer, 99.9% D2O | 99.9% D2O
NMR sample composition 1.0mM [U-15N] TITIN I27 mutant, 50mM potassium phosphate buffer, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 1.0mM [U-13C; U-15N] TITIN I27 mutant, 50mM potassium phosphate buffer, 99.9% D2O | 99.9% D2O
Resolution not provided
2WP3 Crystal structure of the Titin M10-Obscurin like 1 Ig complex Deposited 2009-08-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain T 34252–34350（99 aa） Fragment：M10, RESIDUES 34252-34350
Not recorded GOL GLYCEROL × 4 SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；PROTEIN COMPLEX AT 13 MG/ML 2M AMM SULPHATE, 0.1 M BIS-TRIS PH 5.5
Resolution 1.48 Å R-free 0.217
2WWK Crystal structure of the Titin M10-Obscurin like 1 Ig F17R mutant complex Deposited 2009-10-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain T 34252–34350（99 aa） Fragment：M10, RESIDUES 34252-34350
Not recorded GOL GLYCEROL × 1 SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 5.5；0.1 M BIS-TRIS PH 5.5, 0.2 M AMMONIUM SULPHATE, 25% PEG 3350
Resolution 1.70 Å R-free 0.259
2WWM Crystal structure of the Titin M10-Obscurin like 1 Ig complex in space group P1 Deposited 2009-10-26 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain T 34252–34350（99 aa） Fragment：M10, RESIDUES 34252-34350
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 5；0.1 M MIB BUFFER PH 5.0, 25% PEG 1500
Resolution 2.30 Å R-free 0.252
2WWM Crystal structure of the Titin M10-Obscurin like 1 Ig complex in space group P1 Deposited 2009-10-26 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain D 34252–34350（99 aa） Fragment：M10, RESIDUES 34252-34350
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 5；0.1 M MIB BUFFER PH 5.0, 25% PEG 1500
Resolution 2.30 Å R-free 0.252
2Y9R Crystal structure of the M10 domain of Titin Deposited 2011-02-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain T 26828–26926（99 aa） Fragment：M10 DOMAIN, RESIDUES 26828-26926
Not recorded 12P DODECAETHYLENE GLYCOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；35% PEG 400, 0.1M BIS-TRIS PH 6.5
Resolution 1.90 Å R-free 0.241
3KNB Crystal structure of the titin C-terminus in complex with obscurin-like 1 Deposited 2009-11-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 34253–34350（98 aa） Fragment：titin, C-terminal domain M10, resideus 34253-34350
Not recorded SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；ammonium sulfate, imidazole, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.40 Å R-free 0.195
3LCY Titin Ig tandem domains A164-A165 Deposited 2010-01-12 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 31456–31649（194 aa） Fragment：Titin A-band tandem Ig domains A164-A165
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；0.1M Hepes PH 7.5, 10% iso-propanol 8.5% PEG20,000, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.50 Å R-free 0.240
3LCY Titin Ig tandem domains A164-A165 Deposited 2010-01-12 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 31456–31649（194 aa） Fragment：Titin A-band tandem Ig domains A164-A165
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；0.1M Hepes PH 7.5, 10% iso-propanol 8.5% PEG20,000, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.50 Å R-free 0.240
3LCY Titin Ig tandem domains A164-A165 Deposited 2010-01-12 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 31456–31649（194 aa） Fragment：Titin A-band tandem Ig domains A164-A165
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；0.1M Hepes PH 7.5, 10% iso-propanol 8.5% PEG20,000, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.50 Å R-free 0.240
3LCY Titin Ig tandem domains A164-A165 Deposited 2010-01-12 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain D 31456–31649（194 aa） Fragment：Titin A-band tandem Ig domains A164-A165
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；0.1M Hepes PH 7.5, 10% iso-propanol 8.5% PEG20,000, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.50 Å R-free 0.240
3LPW Crystal structure of the FnIII-tandem A77-A78 from the A-band of titin Deposited 2010-02-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 22877–23070（194 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；277 K；VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.65 Å R-free 0.208
3LPW Crystal structure of the FnIII-tandem A77-A78 from the A-band of titin Deposited 2010-02-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 22877–23070（194 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；277 K；VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.65 Å R-free 0.208
3PUC Atomic resolution structure of titin domain M7 Deposited 2010-12-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 33774–33871（98 aa） Fragment：domain M7 (UNP RESIDUES 33774-33871)
Not recorded SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；0.1M Tris pH 8.0, 3.2M ammonium sulfate, 1mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 0.96 Å R-free 0.135
3Q5O Crystal structure of human titin domain M10 Deposited 2010-12-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 34253–34350（98 aa） Fragment：domain M10 (UNP RESIDUES 34253-34350)
Not recorded SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；277 K；0.1M bistris p 6.8, 55% MPD, 0.2M magnesium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.05 Å R-free 0.215
3Q5O Crystal structure of human titin domain M10 Deposited 2010-12-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 34253–34350（98 aa） Fragment：domain M10 (UNP RESIDUES 34253-34350)
Not recorded SO4 SULFATE ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；277 K；0.1M bistris p 6.8, 55% MPD, 0.2M magnesium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.05 Å R-free 0.215
3QP3 Crystal structure of titin domain M4, tetragonal form Deposited 2011-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 33294–33395（102 aa） Fragment：domain M4 (UNP RESIDUES 33294-33395)
Not recorded SO4 SULFATE ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；293 K；0.2M Na-citrate pH 4.0, 1.2M (NH4)2SO4, 0.3M MgSO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.00 Å R-free 0.213
3QP3 Crystal structure of titin domain M4, tetragonal form Deposited 2011-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 33294–33395（102 aa） Fragment：domain M4 (UNP RESIDUES 33294-33395)
Not recorded SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；293 K；0.2M Na-citrate pH 4.0, 1.2M (NH4)2SO4, 0.3M MgSO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.00 Å R-free 0.213
3QP3 Crystal structure of titin domain M4, tetragonal form Deposited 2011-02-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 33294–33395（102 aa） Fragment：domain M4 (UNP RESIDUES 33294-33395)
Not recorded SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；293 K；0.2M Na-citrate pH 4.0, 1.2M (NH4)2SO4, 0.3M MgSO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.00 Å R-free 0.213
4C4K Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2013-09-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain T 34252–34350（99 aa） Fragment：M10 DOMAIN, RESIDUES 34252-34350
Not recorded EDO 1,2-ETHANEDIOL × 12 X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.5；0.1 M TRIS-HCL PH 8.5, 0.2 M SODIUM ACETATE, 30% PEG4000
Resolution 1.95 Å R-free 0.210
4JNW Bacterially expressed Titin Kinase Deposited 2013-03-16 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 32172–32492（321 aa） Fragment：titin kinase: unp residues 32172-32492
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；VAPOR DIFFUSION, HANGING DROP
Resolution 2.06 Å R-free 0.204
4JNW Bacterially expressed Titin Kinase Deposited 2013-03-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 32172–32492（321 aa） Fragment：titin kinase: unp residues 32172-32492
Not recorded GOL GLYCEROL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；VAPOR DIFFUSION, HANGING DROP
Resolution 2.06 Å R-free 0.204
4O00 Crystal structure of the Titin A-band domain A3 Deposited 2013-12-13 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 14217–14319（103 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；291 K；0.1M SPG buffer, 0.01M Tris-HCl, 31% PEG1500, 50mM NaCl, 1mM DTT, pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K
Resolution 1.85 Å R-free 0.208
4O00 Crystal structure of the Titin A-band domain A3 Deposited 2013-12-13 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 14217–14319（103 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；291 K；0.1M SPG buffer, 0.01M Tris-HCl, 31% PEG1500, 50mM NaCl, 1mM DTT, pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K
Resolution 1.85 Å R-free 0.208
4QEG Crystal structure of domain I10 from titin (space group P41) Deposited 2014-05-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 2880–2967（88 aa） Fragment：domain I10
Not recorded CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；0.2 M CaCl2, 30% PEG 3350, 0.1 M Tris, 3% Isopropanol, pH 7.5, VAPOR DIFFUSION, SITTING DROP
Resolution 2.00 Å R-free 0.231
4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain Y 34253–34349（97 aa） Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
Not recorded CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
Resolution 3.30 Å R-free 0.210
4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 10 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain O 34253–34349（97 aa） Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
Not recorded NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
Resolution 3.30 Å R-free 0.210
4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 11 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain K 34253–34349（97 aa） Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
Resolution 3.30 Å R-free 0.210
4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 12 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain U 34253–34349（97 aa） Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
Resolution 3.30 Å R-free 0.210
4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 13 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain G 34253–34349（97 aa） Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
Resolution 3.30 Å R-free 0.210
4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 14 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain Q 34253–34349（97 aa） Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
Not recorded CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
Resolution 3.30 Å R-free 0.210
4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 15 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain W 34253–34349（97 aa） Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
Not recorded CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
Resolution 3.30 Å R-free 0.210
4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 16 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain 2 34253–34349（97 aa） Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
Resolution 3.30 Å R-free 0.210
4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 17 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 34253–34349（97 aa） Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
Not recorded CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
Resolution 3.30 Å R-free 0.210
4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 18 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain S 34253–34349（97 aa） Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
Not recorded CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
Resolution 3.30 Å R-free 0.210
4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain M 34253–34349（97 aa） Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
Not recorded CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
Resolution 3.30 Å R-free 0.210
4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain E 34253–34349（97 aa） Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
Not recorded CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
Resolution 3.30 Å R-free 0.210
4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain 8 34253–34349（97 aa） Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
Resolution 3.30 Å R-free 0.210
4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 5 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain 6 34253–34349（97 aa） Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
Resolution 3.30 Å R-free 0.210
4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 6 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain 0 34253–34349（97 aa） Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
Not recorded CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
Resolution 3.30 Å R-free 0.210
4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 7 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain I 34253–34349（97 aa） Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
Resolution 3.30 Å R-free 0.210
4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 8 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 34253–34349（97 aa） Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
Resolution 3.30 Å R-free 0.210
4UOW Crystal structure of the titin M10-Obscurin Ig domain 1 complex Deposited 2014-06-10 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 9 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain 4 34253–34349（97 aa） Fragment：FIRST IG DOMAIN, RESIDUES 26829-26925
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 3.6；291 K；3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP
Resolution 3.30 Å R-free 0.210
5BS0 MAGE-A3 Reactive TCR in complex with Titin Epitope in HLA-A1 Deposited 2015-06-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 5 PDB declaration: pentameric
Chain C 24337–24345（9 aa） Fragment：UNP residues 24337-24345
Not recorded GOL GLYCEROL × 1 SO4 SULFATE ION × 11 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；291 K；0.2M Ammonium Chloride, 0.1M MES, 20% PEG 4000
Resolution 2.40 Å R-free 0.262
5JDD Crystal structure of I9-I11 tandem from titin (P212121) Deposited 2016-04-16 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 2795–3053（259 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；292 K；0.1 M Bis-Tris Propane pH 8.5, 20% [w/v] PEG 3350, 0.2 M sodium acetate
Resolution 1.53 Å R-free 0.197
5JDE Crystal structure of I9-I11 tandem from titin (P1) Deposited 2016-04-16 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 2795–3053（259 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；292 K；0.1 M Tris HCl pH 8.5, 30% [w/v] PEG 4000, 0.2 M MgCl2
Resolution 1.90 Å R-free 0.219
5JDE Crystal structure of I9-I11 tandem from titin (P1) Deposited 2016-04-16 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 2795–3053（259 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；292 K；0.1 M Tris HCl pH 8.5, 30% [w/v] PEG 4000, 0.2 M MgCl2
Resolution 1.90 Å R-free 0.219
5JDJ Crystal structure of domain I10 from titin in space group P212121 Deposited 2016-04-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 2880–2967（88 aa）
Chain L 2880–2967（88 aa）
Not recorded CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；292 K；0.2 M CaCl2, 0.1 M Tris pH 7.5, 25% [w/v] PEG 8000
Resolution 1.74 Å R-free 0.224
5JDJ Crystal structure of domain I10 from titin in space group P212121 Deposited 2016-04-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain B 2880–2967（88 aa）
Chain P 2880–2967（88 aa）
Not recorded CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；292 K；0.2 M CaCl2, 0.1 M Tris pH 7.5, 25% [w/v] PEG 8000
Resolution 1.74 Å R-free 0.224
5JDJ Crystal structure of domain I10 from titin in space group P212121 Deposited 2016-04-16 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 2880–2967（88 aa）
Chain M 2880–2967（88 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；292 K；0.2 M CaCl2, 0.1 M Tris pH 7.5, 25% [w/v] PEG 8000
Resolution 1.74 Å R-free 0.224
5JDJ Crystal structure of domain I10 from titin in space group P212121 Deposited 2016-04-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain D 2880–2967（88 aa）
Chain E 2880–2967（88 aa）
Not recorded CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；292 K；0.2 M CaCl2, 0.1 M Tris pH 7.5, 25% [w/v] PEG 8000
Resolution 1.74 Å R-free 0.224
5JDJ Crystal structure of domain I10 from titin in space group P212121 Deposited 2016-04-16 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 5 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain F 2880–2967（88 aa）
Chain N 2880–2967（88 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；292 K；0.2 M CaCl2, 0.1 M Tris pH 7.5, 25% [w/v] PEG 8000
Resolution 1.74 Å R-free 0.224
5JDJ Crystal structure of domain I10 from titin in space group P212121 Deposited 2016-04-16 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 6 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain G 2880–2967（88 aa）
Chain J 2880–2967（88 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；292 K；0.2 M CaCl2, 0.1 M Tris pH 7.5, 25% [w/v] PEG 8000
Resolution 1.74 Å R-free 0.224
5JDJ Crystal structure of domain I10 from titin in space group P212121 Deposited 2016-04-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 7 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain H 2880–2967（88 aa）
Chain I 2880–2967（88 aa）
Not recorded CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；292 K；0.2 M CaCl2, 0.1 M Tris pH 7.5, 25% [w/v] PEG 8000
Resolution 1.74 Å R-free 0.224
5JDJ Crystal structure of domain I10 from titin in space group P212121 Deposited 2016-04-16 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 8 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain K 2880–2967（88 aa）
Chain O 2880–2967（88 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；292 K；0.2 M CaCl2, 0.1 M Tris pH 7.5, 25% [w/v] PEG 8000
Resolution 1.74 Å R-free 0.224
5JOE Crystal structure of I81 from titin Deposited 2016-05-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 9582–9671（90 aa） Fragment：I81 domain
Not recorded IPA ISOPROPYL ALCOHOL × 1 EDO 1,2-ETHANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；292.15 K；0.1M Tris pH 8.5; 30% w/v PEG 4000
Resolution 2.00 Å R-free 0.218
6DL4 Human Titin ZIg10 Deposited 2018-05-31 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1821–1931（111 aa）
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 7.5；298 K；Ionic strength (raw mmCIF value) 50；Pressure 1
NMR sample composition 20 mM TRIS, 50 mM sodium chloride, 1 mM [U-99% 13C; U-99% 15N] Human Titin ZIg10, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
6FWX Chimeric titin Z1Z2-Z1Z2 tandem (Z1212) functionalized with a GRGDS exogenous peptide from fibronectin Deposited 2018-03-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–196（196 aa）
Chain A 2–47（46 aa）
Chain A 52–196（145 aa）
Not recorded EDO 1,2-ETHANEDIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；10% [v/v] isopropanol, 100 mM Na2HPO4 pH 4.2, 200 mM LiSO4
Resolution 3.00 Å R-free 0.226
6FWX Chimeric titin Z1Z2-Z1Z2 tandem (Z1212) functionalized with a GRGDS exogenous peptide from fibronectin Deposited 2018-03-07 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1–196（196 aa）
Chain B 2–47（46 aa）
Chain B 52–196（145 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；10% [v/v] isopropanol, 100 mM Na2HPO4 pH 4.2, 200 mM LiSO4
Resolution 3.00 Å R-free 0.226
6H4L Structure of Titin M4 trigonal form Deposited 2018-07-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 33294–33395（102 aa）
Not recorded ZN ZINC ION × 1 CL CHLORIDE ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；MES pH 6.5 2M RbBr
Resolution 1.60 Å R-free 0.221
6HCI Crystal structure of titin M3 domain Deposited 2018-08-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 32712–32816（105 aa）
Chain B 32712–32816（105 aa）
Chain C 32712–32816（105 aa）
Chain D 32712–32816（105 aa）
Not recorded SO4 SULFATE ION × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；0.2 M ammonium sulfate 23% w/v polyethylene glycol 4000
Resolution 2.12 Å R-free 0.245
6I0Y TnaC-stalled ribosome complex with the titin I27 domain folding close to the ribosomal exit tunnel Deposited 2018-10-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 36-meric
Chain z 14318–14406（89 aa）
Not recorded MG MAGNESIUM ION × 143 ZN ZINC ION × 1 TRP TRYPTOPHAN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6SDB Chimeric titin Z1Z2 functionalized with a KLER exogenous peptide from decorin Deposited 2019-07-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–45（43 aa）
Chain A 51–196（146 aa）
Not recorded ACT ACETATE ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；295 K；1 M NH4H2PO4 pH 4.6, 100 mM sodium acetate
Resolution 2.80 Å R-free 0.234
6SDB Chimeric titin Z1Z2 functionalized with a KLER exogenous peptide from decorin Deposited 2019-07-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain B 3–45（43 aa）
Chain B 51–196（146 aa）
Not recorded ACT ACETATE ION × 1 SO4 SULFATE ION × 1 EDO 1,2-ETHANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；295 K；1 M NH4H2PO4 pH 4.6, 100 mM sodium acetate
Resolution 2.80 Å R-free 0.234
6SDB Chimeric titin Z1Z2 functionalized with a KLER exogenous peptide from decorin Deposited 2019-07-26 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain C 3–45（43 aa）
Chain C 51–196（146 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；295 K；1 M NH4H2PO4 pH 4.6, 100 mM sodium acetate
Resolution 2.80 Å R-free 0.234
6SDB Chimeric titin Z1Z2 functionalized with a KLER exogenous peptide from decorin Deposited 2019-07-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain D 3–45（43 aa）
Chain D 51–196（146 aa）
Not recorded ACT ACETATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；295 K；1 M NH4H2PO4 pH 4.6, 100 mM sodium acetate
Resolution 2.80 Å R-free 0.234
6SDB Chimeric titin Z1Z2 functionalized with a KLER exogenous peptide from decorin Deposited 2019-07-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 5 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain E 3–45（43 aa）
Chain E 51–196（146 aa）
Not recorded ACT ACETATE ION × 3 PO4 PHOSPHATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；295 K；1 M NH4H2PO4 pH 4.6, 100 mM sodium acetate
Resolution 2.80 Å R-free 0.234
6YGN Titin kinase and its flanking domains Deposited 2020-03-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 30596–31144（549 aa）
Not recorded EDO 1,2-ETHANEDIOL × 1 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293.15 K；30% PEG 8K, 0.1 MES pH6.5
Resolution 2.40 Å R-free 0.228
6YGN Titin kinase and its flanking domains Deposited 2020-03-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 30596–31144（549 aa）
Not recorded EDO 1,2-ETHANEDIOL × 1 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293.15 K；30% PEG 8K, 0.1 MES pH6.5
Resolution 2.40 Å R-free 0.228
7AHS titin-N2A Ig81-Ig83 Deposited 2020-09-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 9582–9851（270 aa）
Not recorded EDO 1,2-ETHANEDIOL × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293.15 K；25 % w/v Polyethylene glycol 3,350, 100 mM BIS-TRIS pH 5.5, 200 mM lithium sulfate
Resolution 2.05 Å R-free 0.235
7AHS titin-N2A Ig81-Ig83 Deposited 2020-09-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 9582–9851（270 aa）
Not recorded EDO 1,2-ETHANEDIOL × 7 SO4 SULFATE ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293.15 K；25 % w/v Polyethylene glycol 3,350, 100 mM BIS-TRIS pH 5.5, 200 mM lithium sulfate
Resolution 2.05 Å R-free 0.235
7AHS titin-N2A Ig81-Ig83 Deposited 2020-09-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 9582–9851（270 aa）
Not recorded EDO 1,2-ETHANEDIOL × 3 SO4 SULFATE ION × 2 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293.15 K；25 % w/v Polyethylene glycol 3,350, 100 mM BIS-TRIS pH 5.5, 200 mM lithium sulfate
Resolution 2.05 Å R-free 0.235
7AHS titin-N2A Ig81-Ig83 Deposited 2020-09-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain D 9582–9851（270 aa）
Not recorded EDO 1,2-ETHANEDIOL × 4 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293.15 K；25 % w/v Polyethylene glycol 3,350, 100 mM BIS-TRIS pH 5.5, 200 mM lithium sulfate
Resolution 2.05 Å R-free 0.235
7NIP titin N2A unique sequence (UN2A) core Deposited 2021-02-13 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 8578–8617（40 aa）
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 7.5；298 K；Ionic strength (raw mmCIF value) 100；Pressure 101300
NMR sample composition 30 mM sodium phosphate, 100 mM sodium chloride, 95% H2O/5% D2O | 95% H2O/5% D2O
Resolution not provided
8BNQ Crystal structure of the FnIII-tandem A84-A86 from the A-band of titin Deposited 2022-11-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 23562–23862（301 aa）
Not recorded EDO 1,2-ETHANEDIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；277.15 K；5% [v/v] polyethylene glycol 400 and 40% [v/v] 2-methyl-2,4-pentanediol (MPD)
Resolution 2.30 Å R-free 0.258
8BNQ Crystal structure of the FnIII-tandem A84-A86 from the A-band of titin Deposited 2022-11-14 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 23562–23862（301 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；277.15 K；5% [v/v] polyethylene glycol 400 and 40% [v/v] 2-methyl-2,4-pentanediol (MPD)
Resolution 2.30 Å R-free 0.258
8BVO Titin I110-I111 FnIII tandem from the MIR region (I/A5-I/A6) Deposited 2022-12-05 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 14415–14609（195 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291.15 K；24% (w/v) PEG 1500, 20% (w/v) glycerol
Resolution 2.55 Å R-free 0.287
8BW6 Titin FnIII-domain I110 (I/A6) from the MIR region Deposited 2022-12-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 14415–14509（95 aa）
Not recorded EDO 1,2-ETHANEDIOL × 7 PEG DI(HYDROXYETHYL)ETHER × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291.15 K；0.1M Tris pH8 30% PEG 300
Resolution 1.95 Å R-free 0.254
8BXR Titin FnIII-domain I109-I111 (I/A4-A/A6) from the MIR region Deposited 2022-12-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 14319–14615（297 aa）
Not recorded EDO 1,2-ETHANEDIOL × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291.15 K；0.1M MES pH 6, 10% [w/v] PEG 6000
Resolution 2.70 Å R-free 0.271
8OIY Crystal structure of the R15908H missense variant of titin domain Fn3-3 Deposited 2023-03-23 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain A 14217–14319（103 aa）
Chain B 14217–14319（103 aa）
Chain C 14217–14319（103 aa）
Chain D 14217–14319（103 aa）
Chain E 14217–14319（103 aa）
Chain F 14217–14319（103 aa）
Mutation：R15908H Mutation：R15908H Mutation：R15908H Mutation：R15908H Mutation：R15908H Mutation：R15908H No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；10mM Tris-HCl PH 7.5 NaCl 0.5mM DTT 0.1M sodium acetate pH 4.5 0.2M Lithium sulphate 50% v/v PEG 400
Resolution 2.10 Å R-free 0.257
8OMW Crystal structure of the titin domain Fn3-20 Deposited 2023-03-31 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 16525–16630（106 aa）
Not recorded CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；10mM Tris-HCl pH 7.5 50mM NaCl 0.5mM DTT 0.1M TBG pH 9.0 25% w/v PEG 1500
Resolution 1.05 Å R-free 0.177
8OQ9 Crystal structure of the titin domain Fn3-56 Deposited 2023-04-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 21497–21598（102 aa）
Not recorded ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；10mM Tris-HCl pH 7.5 50mM NaCl 0.5mM DTT 0.1M MES 10mM ZnCl2 20% (w/v) PEG6000
Resolution 1.65 Å R-free 0.192
8OQ9 Crystal structure of the titin domain Fn3-56 Deposited 2023-04-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 21497–21598（102 aa）
Not recorded ZN ZINC ION × 3 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；10mM Tris-HCl pH 7.5 50mM NaCl 0.5mM DTT 0.1M MES 10mM ZnCl2 20% (w/v) PEG6000
Resolution 1.65 Å R-free 0.192
8OQ9 Crystal structure of the titin domain Fn3-56 Deposited 2023-04-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 21497–21598（102 aa）
Not recorded ZN ZINC ION × 1 CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；10mM Tris-HCl pH 7.5 50mM NaCl 0.5mM DTT 0.1M MES 10mM ZnCl2 20% (w/v) PEG6000
Resolution 1.65 Å R-free 0.192
8ORL Crystal structure of the S23226G missense variant of titin domain Fn3-56 Deposited 2023-04-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 21497–21598（102 aa）
Not recorded ZN ZINC ION × 1 CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；10mM Tris-HCl pH 7.5 50mM NaCl 0.5mM DTT 0.1M MES 10mM ZnCl2 20% (w/v) PEG6000
Resolution 1.43 Å R-free 0.194
8ORL Crystal structure of the S23226G missense variant of titin domain Fn3-56 Deposited 2023-04-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 21497–21598（102 aa）
Not recorded ZN ZINC ION × 3 CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；10mM Tris-HCl pH 7.5 50mM NaCl 0.5mM DTT 0.1M MES 10mM ZnCl2 20% (w/v) PEG6000
Resolution 1.43 Å R-free 0.194
8ORL Crystal structure of the S23226G missense variant of titin domain Fn3-56 Deposited 2023-04-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 21497–21598（102 aa）
Not recorded ZN ZINC ION × 1 CL CHLORIDE ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；10mM Tris-HCl pH 7.5 50mM NaCl 0.5mM DTT 0.1M MES 10mM ZnCl2 20% (w/v) PEG6000
Resolution 1.43 Å R-free 0.194
8OS3 Crystal structure of the titin domain Fn3-11 Deposited 2023-04-17 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 15210–15313（104 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；30mM HEPES pH 7.5 50mM NaCl 0.5mM DTT 100mM sodium acetate pH 4.5 200mM Lithium sulphate 50% v/v PEG 400
Resolution 1.68 Å R-free 0.208
8OSD Crystal structure of the titin domain Fn3-49 Deposited 2023-04-18 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 20511–20616（106 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；30mM HEPES pH 7.5 150mM NaCl 1mM DTT 100mM Potassium thiocyanate 30% w/v PEG MME2000
Resolution 1.70 Å R-free 0.260
8OSD Crystal structure of the titin domain Fn3-49 Deposited 2023-04-18 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 20511–20616（106 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；30mM HEPES pH 7.5 150mM NaCl 1mM DTT 100mM Potassium thiocyanate 30% w/v PEG MME2000
Resolution 1.70 Å R-free 0.260
8OT5 Crystal structure of the titin domain Fn3-85 Deposited 2023-04-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 25430–25531（102 aa）
Not recorded NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；30mM HEPES pH 7.5 150mM NaCl 1mM DTT 100mM Bis-Tris propane pH 6.5 200mM sodium iodide 20% w/v PEG3350
Resolution 1.56 Å R-free 0.191
8OT5 Crystal structure of the titin domain Fn3-85 Deposited 2023-04-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 25430–25531（102 aa）
Not recorded NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；30mM HEPES pH 7.5 150mM NaCl 1mM DTT 100mM Bis-Tris propane pH 6.5 200mM sodium iodide 20% w/v PEG3350
Resolution 1.56 Å R-free 0.191
8OT5 Crystal structure of the titin domain Fn3-85 Deposited 2023-04-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 25430–25531（102 aa）
Not recorded NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；30mM HEPES pH 7.5 150mM NaCl 1mM DTT 100mM Bis-Tris propane pH 6.5 200mM sodium iodide 20% w/v PEG3350
Resolution 1.56 Å R-free 0.191
8OT5 Crystal structure of the titin domain Fn3-85 Deposited 2023-04-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain D 25430–25531（102 aa）
Not recorded NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；30mM HEPES pH 7.5 150mM NaCl 1mM DTT 100mM Bis-Tris propane pH 6.5 200mM sodium iodide 20% w/v PEG3350
Resolution 1.56 Å R-free 0.191
8OT5 Crystal structure of the titin domain Fn3-85 Deposited 2023-04-20 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 5 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain E 25430–25531（102 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；30mM HEPES pH 7.5 150mM NaCl 1mM DTT 100mM Bis-Tris propane pH 6.5 200mM sodium iodide 20% w/v PEG3350
Resolution 1.56 Å R-free 0.191
8OT5 Crystal structure of the titin domain Fn3-85 Deposited 2023-04-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 6 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain F 25430–25531（102 aa）
Not recorded CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；30mM HEPES pH 7.5 150mM NaCl 1mM DTT 100mM Bis-Tris propane pH 6.5 200mM sodium iodide 20% w/v PEG3350
Resolution 1.56 Å R-free 0.191
8OTY Crystal structure of the titin domain Fn3-90 Deposited 2023-04-21 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 26124–26224（101 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；30mM HEPES pH 7.5 150mM NaCl 1mM DTT 100mM Sodium acetate 200mM CaCl 20% w/v PEG6000
Resolution 1.90 Å R-free 0.251
8OTY Crystal structure of the titin domain Fn3-90 Deposited 2023-04-21 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 26124–26224（101 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；30mM HEPES pH 7.5 150mM NaCl 1mM DTT 100mM Sodium acetate 200mM CaCl 20% w/v PEG6000
Resolution 1.90 Å R-free 0.251
8OTY Crystal structure of the titin domain Fn3-90 Deposited 2023-04-21 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 26124–26224（101 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；30mM HEPES pH 7.5 150mM NaCl 1mM DTT 100mM Sodium acetate 200mM CaCl 20% w/v PEG6000
Resolution 1.90 Å R-free 0.251
8OTY Crystal structure of the titin domain Fn3-90 Deposited 2023-04-21 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain D 26124–26224（101 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；30mM HEPES pH 7.5 150mM NaCl 1mM DTT 100mM Sodium acetate 200mM CaCl 20% w/v PEG6000
Resolution 1.90 Å R-free 0.251
8OVU Human titin immunoglobulin-like 21 domain Deposited 2023-04-26 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3500–3598（99 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.06 M divalent ions (CaCl2, MgCl2), 0.1 M sodium HEPES, pH 7.5, 50% v/v Polyethylene glycol monomethyl ether (PEGMME) 550, PEG 20K from Morpheus HT-96 screening plate (Molecular Dimensions)
Resolution 1.95 Å R-free 0.239
8OVU Human titin immunoglobulin-like 21 domain Deposited 2023-04-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 3500–3598（99 aa）
Not recorded MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.06 M divalent ions (CaCl2, MgCl2), 0.1 M sodium HEPES, pH 7.5, 50% v/v Polyethylene glycol monomethyl ether (PEGMME) 550, PEG 20K from Morpheus HT-96 screening plate (Molecular Dimensions)
Resolution 1.95 Å R-free 0.239
8P35 Mutant human titin immunoglobulin-like 21 domain - C3575S Deposited 2023-05-17 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3500–3598（99 aa）
Mutation：C3575S No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1 M HEPES sodium salt, pH 7.5, 25 %(w/v) PEG 2000 MME
Resolution 2.20 Å R-free 0.250
8P35 Mutant human titin immunoglobulin-like 21 domain - C3575S Deposited 2023-05-17 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 3500–3598（99 aa）
Mutation：C3575S No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1 M HEPES sodium salt, pH 7.5, 25 %(w/v) PEG 2000 MME
Resolution 2.20 Å R-free 0.250
8P35 Mutant human titin immunoglobulin-like 21 domain - C3575S Deposited 2023-05-17 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 3500–3598（99 aa）
Mutation：C3575S No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1 M HEPES sodium salt, pH 7.5, 25 %(w/v) PEG 2000 MME
Resolution 2.20 Å R-free 0.250
8P35 Mutant human titin immunoglobulin-like 21 domain - C3575S Deposited 2023-05-17 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain D 3500–3598（99 aa）
Mutation：C3575S No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1 M HEPES sodium salt, pH 7.5, 25 %(w/v) PEG 2000 MME
Resolution 2.20 Å R-free 0.250
8P35 Mutant human titin immunoglobulin-like 21 domain - C3575S Deposited 2023-05-17 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 5 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain E 3500–3598（99 aa）
Mutation：C3575S No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1 M HEPES sodium salt, pH 7.5, 25 %(w/v) PEG 2000 MME
Resolution 2.20 Å R-free 0.250
8P35 Mutant human titin immunoglobulin-like 21 domain - C3575S Deposited 2023-05-17 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 6 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain F 3500–3598（99 aa）
Mutation：C3575S No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1 M HEPES sodium salt, pH 7.5, 25 %(w/v) PEG 2000 MME
Resolution 2.20 Å R-free 0.250

54 other PDB entries and 123 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name TITIN_HUMAN
Isoform —
PDB entities 4
Chains and sequence ranges Author chain am； PDBConstruct 1–1084； UniProt 22784–23867 Author chain an； PDBConstruct 1–1084； UniProt 22784–23867 Author chain bm； PDBConstruct 1–1084； UniProt 22784–23867 Author chain bn； PDBConstruct 1–1084； UniProt 22784–23867 Author chain m； PDBConstruct 1–1084； UniProt 22784–23867 Author chain n； PDBConstruct 1–1084； UniProt 22784–23867

Myosin-binding protein C, cardiac-type

OrganismNot specified

UniProt Q14896

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 123 PDB declaration: 123-meric（123） Consistent with protein copy count Chain ao； UniProt 1–1274 Chain bo； UniProt 1–1274 Chain o； UniProt 1–1274 Not recorded Myosin-7 × 78 （P12883） Myosin light chain 3 × 18 （P08590） Myosin regulatory light chain 2, ventricular/cardiac muscle isoform × 18 （P10916） Titin × 6 （Q8WZ42） ELECTRON MICROSCOPY cryo-EM buffer：pH 6.8 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 6.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8G4L Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1GXE Central domain of cardiac myosin binding protein C Deposited 2002-04-03 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 641–770（130 aa） Fragment：DOMAIN C5, RESIDUES 641-770
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 7；293 K；Ionic strength (raw mmCIF value) 100 MM NACL；Pressure 1
Resolution not provided
1PD6 The NMR structure of domain C2 of human cardiac Myosin Binding Protein C Deposited 2003-05-19 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 358–451（94 aa） Fragment：Domain C2
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 7；298 K；Ionic strength (raw mmCIF value) 0.1；Pressure ambient
NMR measurement conditions pH 7；298 K；Ionic strength (raw mmCIF value) 0.1；Pressure ambient
NMR measurement conditions pH 7；298 K；Ionic strength (raw mmCIF value) 7.0；Pressure ambient
NMR sample composition 0.75mM C2; 15N; 20mM phosphate buffer; 90% H2O, 10% D2O | 90% H2O/10% D2O
NMR sample composition 0.75mM C2; 15N, 13C; 20mM phosphate buffer; 90% H2O, 10% D2O | 90% H2O/10% D2O
NMR sample composition 0.75mM C2; 15N, 13C; 20mM phosphate buffer; 100% D2O | 100% D2O
Resolution not provided
2AVG NMR structure of cC1 domain from Human Cardiac Myosin Binding Protein C Deposited 2005-08-30 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 151–260（110 aa） Fragment：cC1 domain
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 7；298 K；Ionic strength (raw mmCIF value) 0.1M；Pressure ambient
NMR sample composition 0.8mM cC1 U-15N; 20mM phosphate buffer; 90% H2O, 10% D2O | 90% H2O/10% D2O
NMR sample composition 0.8mM cC1 U-15N, 13C; 20mM phosphate buffer; 90% H2O, 10% D2O | 90% H2O/10% D2O
NMR sample composition 0.8mM cC1 U-15N, 13C; 20mM phosphate buffer; 100% D2O | 100% D2O
Resolution not provided
2K1M 3D NMR structure of domain cC0 of cardiac myosin binding protein C (MyBPC) Deposited 2008-03-10 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 2–96（95 aa）
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 7；303 K；Ionic strength (raw mmCIF value) 185；Pressure ambient
NMR sample composition 1-1.5 mM [U-95% 13C; U-95% 15N] cC0_MyBPC, 55.5 M [U-2H] D2O, 40 mM sodium phosphate, 50 mM sodium chloride, 2 mM DTT, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 1-1.5 mM [U-95% 15N] cC0_MyBPC, 55.5 M H2O, 40 mM sodium phosphate, 50 mM sodium chloride, 2 mM DTT, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 1-1.5 mM cC0_MyBPC, 55.5 M H2O, 40 mM sodium phosphate, 50 mM sodium chloride, 2 mM DTT, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
2MQ0 NMR structure of the c3 domain of human cardiac myosin binding protein-c Deposited 2014-06-10 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 453–543（91 aa） Fragment：unp residues 453-543
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.5；298 K；Ionic strength (raw mmCIF value) 0.4；Pressure ambient
NMR sample composition 50 mM sodium phosphate, 100 mM sodium chloride, 0.8 mM [U-100% 13C; U-100% 15N] protein, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
2MQ3 NMR structure of the c3 domain of human cardiac myosin binding protein-c with a hypertrophic cardiomyopathy-related mutation R502W. Deposited 2014-06-12 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 453–543（91 aa） Fragment：unp residues 453-543
Mutation：R502W No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.5；298 K；Ionic strength (raw mmCIF value) 0.4；Pressure ambient
NMR sample composition 50 mM sodium phosphate, 100 mM sodium chloride, 0.8 mM [U-100% 13C; U-100% 15N] protein, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
2V6H Crystal structure of the C1 domain of cardiac myosin binding protein-C Deposited 2007-07-18 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 151–258（108 aa） Fragment：C1 DOMAIN, RESIDUES 151-258
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.3；293 K；HANGING DROP; 20 C; 18%PEG, 0.1M BUFFER, PH 7.3
Resolution 1.55 Å R-free 0.241
3CX2 Crystal structure of the C1 domain of cardiac isoform of myosin binding protein-C at 1.3A Deposited 2008-04-23 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 151–258（108 aa） Fragment：Ig-like C2-type 1 domain
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.9；293 K；Crystals were grown at 20 C using the hanging drop method with a 2 microlitre drop containing a 1:1 mixture of protein solution and crystallising solution consisting of 8.5% PEG 3350 and 0.05M Hepes pH 6.8 over 500 microlitres of crystallising solution in the reservoir, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.30 Å R-free 0.209
5K6P The NMR structure of the m domain tri-helix bundle and C2 of human cardiac Myosin Binding Protein C Deposited 2016-05-25 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 319–451（133 aa） Fragment：residues 319-451
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.5；298 K；Ionic strength (raw mmCIF value) 50；Pressure 1
NMR sample composition 412 uM [U-99% 13C; U-99% 15N] human cMyBP-C (319-451), 10 mM MES, 1 mM CaCl2, 50 mM NaCl, 2 mM TCEP, 1 mM Na azide, 10 mM DSS, 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition 920 uM [U-99% 13C; U-99% 15N] human cMyBP-C (319-451), 10 mM MES, 1 mM CaCl2, 50 mM NaCl, 2 mM TCEP, 1 mM Na azide, 10 mM DSS, 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition 550 uM [U-99% 13C; U-99% 15N] human cMyBP-C (319-451), 10 mM MES, 1 mM CaCl2, 50 mM NaCl, 1 mM Na azide, 2 mM TCEP, 10 mM DSS, 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition 500 uM [U-99% 13C; U-99% 15N] human cMyBP-C (319-451), 10 mM MES, 1 mM CaCl2, 50 mM NaCl, 2 mM TCEP, 1 mM Na azide, 10 mM DSS, 99% D2O | 99% D2O
NMR sample composition 800 uM [U-99% 13C; U-99% 15N] human cMyBP-C (319-451), 10 mM MES, 1 mM CaCl2, 50 mM NaCl, 2 mM TCEP, 1 mM Na azide, 10 mM DSS, 99% D2O | 99% D2O
Resolution not provided
6CXI Cardiac thin filament decorated with C0C1 fragment of cardiac myosin binding protein C mode 1 Deposited 2018-04-03 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain G 151–258（108 aa） Fragment：C1 Ig-domain (UNP residues 151-258)
Chain H 151–258（108 aa） Fragment：C1 Ig-domain (UNP residues 151-258)
Chain I 151–258（108 aa） Fragment：C1 Ig-domain (UNP residues 151-258)
Chain J 151–258（108 aa） Fragment：C1 Ig-domain (UNP residues 151-258)
Chain K 151–258（108 aa） Fragment：C1 Ig-domain (UNP residues 151-258)
Chain L 151–258（108 aa） Fragment：C1 Ig-domain (UNP residues 151-258)
Chain M 1–101（101 aa） Fragment：C0 Ig-domain (UNP residues 1-101)
Chain N 1–101（101 aa） Fragment：C0 Ig-domain (UNP residues 1-101)
Chain O 1–101（101 aa） Fragment：C0 Ig-domain (UNP residues 1-101)
Chain P 1–101（101 aa） Fragment：C0 Ig-domain (UNP residues 1-101)
Chain Q 1–101（101 aa） Fragment：C0 Ig-domain (UNP residues 1-101)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 11.00 Å
6CXJ Cardiac thin filament decorated with C0C1 fragment of cardiac myosin binding protein C mode 2 Deposited 2018-04-03 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric
Chain G 151–258（108 aa） Fragment：C1 Ig-domain (UNP residues 151-258)
Chain H 151–258（108 aa） Fragment：C1 Ig-domain (UNP residues 151-258)
Chain I 151–258（108 aa） Fragment：C1 Ig-domain (UNP residues 151-258)
Chain J 151–258（108 aa） Fragment：C1 Ig-domain (UNP residues 151-258)
Chain K 151–258（108 aa） Fragment：C1 Ig-domain (UNP residues 151-258)
Chain L 151–258（108 aa） Fragment：C1 Ig-domain (UNP residues 151-258)
Chain M 1–101（101 aa） Fragment：C0 Ig-domain (UNP residues 1-101)
Chain N 1–101（101 aa） Fragment：C0 Ig-domain (UNP residues 1-101)
Chain O 1–101（101 aa） Fragment：C0 Ig-domain (UNP residues 1-101)
Chain P 1–101（101 aa） Fragment：C0 Ig-domain (UNP residues 1-101)
Chain Q 1–101（101 aa） Fragment：C0 Ig-domain (UNP residues 1-101)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 11.00 Å
6G2T human cardiac myosin binding protein C C1 Ig-domain bound to native cardiac thin filament Deposited 2018-03-23 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain G 151–258（108 aa）
Chain H 151–258（108 aa）
Chain I 151–258（108 aa）
Chain J 151–258（108 aa）
Chain K 151–258（108 aa）
Chain L 151–258（108 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 9.00 Å
7LRG Filamentous actin decorated with human cardiac myosin binding protein C C2 domain Deposited 2021-02-16 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain G 358–451（94 aa）
Chain H 358–451（94 aa）
Chain I 358–451（94 aa）
Chain J 358–451（94 aa）
Chain K 358–451（94 aa）
Chain L 358–451（94 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.10 Å
7TIJ Cardiac F-actin decorated with regulatory M-domain of cardiac myosin binding protein C Deposited 2022-01-13 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain G 151–370（220 aa）
Chain H 151–370（220 aa）
Chain I 151–370（220 aa）
Chain J 151–370（220 aa）
Chain K 151–370（220 aa）
Chain L 151–370（220 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.00 Å
7TIT Cardiac thin filament decorated with regulatory M-domain of cardiac myosin binding protein C Deposited 2022-01-14 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain G 151–370（220 aa）
Chain H 151–370（220 aa）
Chain I 151–370（220 aa）
Chain J 151–370（220 aa）
Chain K 151–370（220 aa）
Chain L 151–370（220 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.00 Å
7TJ7 Cardiac thin filament decorated with C1 Ig-domain and regulatory M-domain of cardiac myosin binding protein C (cMyBP-C) Deposited 2022-01-14 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric
Chain G 151–370（220 aa）
Chain H 151–370（220 aa）
Chain I 151–370（220 aa）
Chain J 151–370（220 aa）
Chain K 151–370（220 aa）
Chain L 151–370（220 aa）
Chain M 151–370（220 aa）
Chain N 151–370（220 aa）
Chain O 151–370（220 aa）
Chain P 151–370（220 aa）
Chain Q 151–370（220 aa）
Chain R 151–370（220 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.00 Å

16 other PDB entries and 16 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name MYPC3_HUMAN
Isoform —
PDB entities 5
Chains and sequence ranges Author chain ao； PDBConstruct 1–1274； UniProt 1–1274 Author chain bo； PDBConstruct 1–1274； UniProt 1–1274 Author chain o； PDBConstruct 1–1274； UniProt 1–1274

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 8g4l

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 8g4l
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2. Structure Basics 2. Structure Basics

Entry ID entry_id8g4l
Deposition date deposition_date2023-02-10
Structure title titleCryo-EM structure of the human cardiac myosin filament
Keywords keywordscardiac, myosin, filament, complex, CONTRACTILE PROTEIN; CONTRACTILE PROTEIN
Experimental Method methodELECTRON MICROSCOPY
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier— Å
Radius of gyration Rg (electron density) rg_electron167.90 Å
Forward intensity I(0) i0489957000000.00
Molecular weight molecular_weight5895500.0 kDa
Excluded volume excluded_volume7344000 ų
Envelope volume envelope_volume16577000 ų
Hydration-shell volume shell_volume840770 ų
Envelope diameter envelope_diameter579.0 Å
Shell Rg shell_rg150.60 Å
Envelope Rg envelope_rg159.40 Å
Shape Rg shape_rg167.90 Å
Total Rg total_rg167.90 Å
Total atoms total_atoms828952
Residues n_residues51438
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax481.2 Å
Rg (real space) rg_real161.40 Å
Rg uncertainty (real space) rg_real_error1.52 Å
I(0) (real space) i0_real4.6510e+11
I(0) uncertainty (real space) i0_real_error1.1650e+10
Rg (reciprocal space) rg_reciprocal153.40 Å
I(0) (reciprocal space) i0_reciprocal458100000000.0000
Solution quality estimate total_estimate0.8987
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks1
Primary peak position r_peak_primary213.9 Å
Skewness Skewness skewness0.385
Kurtosis Kurtosis kurtosis-0.381
Angular range angular_range— – 0.0450 Å−1
Current regularization parameter α current_alpha1.5260
Highest regularization parameter α highest_alpha51220000000.0000
Real-space data points n_real_points10
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.078; Oscil: 0.974; Stabil: 0.942; Sysdev: 1.000; Positv: 1.000; Valcen: 0.996; Smooth: 0.000
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (5)

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8. Citations (1)

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9. Files and Curves (10)