5wme

Crystal Structure of Amino Acids 1729-1786 of Human Beta Cardiac Myosin Fused to Gp7 as Anti-Parallel Four-Helix Bundle

Method: X-RAY DIFFRACTION D_max: 148.2 Å Quality: REASONABLE

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Capsid assembly scaffolding protein,Myosin-7

Homo sapiens

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Insufficient information Homooligomer Protein × 4 PDB declaration: tetrameric（4） Consistent with protein copy count	Chain A； UniProt 1728–1786 Chain B； UniProt 1728–1786 Chain C； UniProt 1728–1786 Chain D； UniProt 1728–1786	Fragment：UNP P13848 residues 2-48, UNP P12883 residues 1729-1786	No other associated polymer	X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 5；298 K；18% (w/v) pentaerythritol ethoxylate 797, 150 mM ammonium thiocyanate, 100 mM sodium acetate pH 5.0.	Resolution 2.30 Å R-free 0.264

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 5WME	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
2FXM Structure of the human beta-myosin S2 fragment Deposited 2006-02-06	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 838–963（126 aa） Fragment：DELTA-S2 FRAGMENT (838-963) Chain B 838–963（126 aa） Fragment：DELTA-S2 FRAGMENT (838-963)	Not recorded	HG MERCURY (II) ION × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；296 K；PEG 3350, LITHIUM CITRATE, TRIS- HCL, pH 8.50, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 296K	Resolution 2.70 Å R-free 0.283
2FXO Structure of the human beta-myosin S2 fragment Deposited 2006-02-06	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 838–963（126 aa） Fragment：DELTA-S2 FRAGMENT (838-963) Chain B 838–963（126 aa） Fragment：DELTA-S2 FRAGMENT (838-963)	Mutation：E924K Mutation：E924K	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；296 K；PEG 3350, SODIUM ACETATE, TRIS-HCL, pH 7.50, VAPOR DIFFUSION, SITTING DROP, temperature 296K	Resolution 2.50 Å R-free 0.349
2FXO Structure of the human beta-myosin S2 fragment Deposited 2006-02-06	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain C 838–963（126 aa） Fragment：DELTA-S2 FRAGMENT (838-963) Chain D 838–963（126 aa） Fragment：DELTA-S2 FRAGMENT (838-963)	Mutation：E924K Mutation：E924K	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；296 K；PEG 3350, SODIUM ACETATE, TRIS-HCL, pH 7.50, VAPOR DIFFUSION, SITTING DROP, temperature 296K	Resolution 2.50 Å R-free 0.349
3DTP Tarantula heavy meromyosin obtained by flexible docking to Tarantula muscle thick filament Cryo-EM 3D-MAP Deposited 2008-07-15	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 216 PDB declaration: 216-meric	Chain A 842–961（120 aa） Fragment：SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-972),SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-972) Chain B 842–963（122 aa） Fragment：SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-974),SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-974)	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer 100mM NaCl, 3mM MgCl2, 1mM EGTA, 5mM PIPES, 5mM NaH2PO4, 1mM NaN3；pH 7；100mM NaCl, 3mM MgCl2, 1mM EGTA, 5mM PIPES, 5mM NaH2PO4, 1mM NaN3 cryo-EM vitrification conditions Plunging in a liquid ethane. Blotting was performed from one side of the grid till a thin sample film on it using Whatman No 42 filter paper, then the grid was immediately plunged under gravity into liquid ethane cooled by liquid nitrogen. Grids were stored under liquid nitrogen.	Resolution 20.00 Å
3DTP Tarantula heavy meromyosin obtained by flexible docking to Tarantula muscle thick filament Cryo-EM 3D-MAP Deposited 2008-07-15	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric	Chain A 842–961（120 aa） Fragment：SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-972),SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-972) Chain B 842–963（122 aa） Fragment：SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-974),SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-974)	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer 100mM NaCl, 3mM MgCl2, 1mM EGTA, 5mM PIPES, 5mM NaH2PO4, 1mM NaN3；pH 7；100mM NaCl, 3mM MgCl2, 1mM EGTA, 5mM PIPES, 5mM NaH2PO4, 1mM NaN3 cryo-EM vitrification conditions Plunging in a liquid ethane. Blotting was performed from one side of the grid till a thin sample film on it using Whatman No 42 filter paper, then the grid was immediately plunged under gravity into liquid ethane cooled by liquid nitrogen. Grids were stored under liquid nitrogen.	Resolution 20.00 Å
3DTP Tarantula heavy meromyosin obtained by flexible docking to Tarantula muscle thick filament Cryo-EM 3D-MAP Deposited 2008-07-15	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 3 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric	Chain A 842–961（120 aa） Fragment：SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-972),SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-972) Chain B 842–963（122 aa） Fragment：SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-974),SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-974)	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer 100mM NaCl, 3mM MgCl2, 1mM EGTA, 5mM PIPES, 5mM NaH2PO4, 1mM NaN3；pH 7；100mM NaCl, 3mM MgCl2, 1mM EGTA, 5mM PIPES, 5mM NaH2PO4, 1mM NaN3 cryo-EM vitrification conditions Plunging in a liquid ethane. Blotting was performed from one side of the grid till a thin sample film on it using Whatman No 42 filter paper, then the grid was immediately plunged under gravity into liquid ethane cooled by liquid nitrogen. Grids were stored under liquid nitrogen.	Resolution 20.00 Å
4DB1 Cardiac human myosin S1dC, beta isoform complexed with Mn-AMPPNP Deposited 2012-01-13	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 2–783（782 aa） Fragment：UNP residues 2-783	Not recorded	ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 MN MANGANESE (II) ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；277 K；precipitant: 7.5% PEG 8000, 0.05M MES, 0.05M acetate, 0.25M sodium chloride, 0.01M manganese chloride, 0.1% sodium cholate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	Resolution 2.60 Å R-free 0.257
4DB1 Cardiac human myosin S1dC, beta isoform complexed with Mn-AMPPNP Deposited 2012-01-13	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain B 2–783（782 aa） Fragment：UNP residues 2-783	Not recorded	ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 MN MANGANESE (II) ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；277 K；precipitant: 7.5% PEG 8000, 0.05M MES, 0.05M acetate, 0.25M sodium chloride, 0.01M manganese chloride, 0.1% sodium cholate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	Resolution 2.60 Å R-free 0.257
4DB1 Cardiac human myosin S1dC, beta isoform complexed with Mn-AMPPNP Deposited 2012-01-13	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 2–783（782 aa） Fragment：UNP residues 2-783 Chain B 2–783（782 aa） Fragment：UNP residues 2-783	Not recorded	ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 MN MANGANESE (II) ION × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；277 K；precipitant: 7.5% PEG 8000, 0.05M MES, 0.05M acetate, 0.25M sodium chloride, 0.01M manganese chloride, 0.1% sodium cholate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	Resolution 2.60 Å R-free 0.257
4P7H Structure of Human beta-Cardiac Myosin Motor Domain::GFP chimera Deposited 2014-03-27	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–787（787 aa） Fragment：UNP P12883 residues 1-787,UNP P42212 residues 5-238	Mutation：Q80R, K101N, V163A, I167T, S175G, D190N Non-standard monomer：Yes (specific site not provided by mmCIF)	SO4 SULFATE ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；10% Tacsimate, pH 6.0, 10% glycerol, 14-15% PEG 3350, 0.2 mM MgCL2, and 5 mM TCEP	Resolution 3.20 Å R-free 0.284
4P7H Structure of Human beta-Cardiac Myosin Motor Domain::GFP chimera Deposited 2014-03-27	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric	Chain B 1–787（787 aa） Fragment：UNP P12883 residues 1-787,UNP P42212 residues 5-238	Mutation：Q80R, K101N, V163A, I167T, S175G, D190N Non-standard monomer：Yes (specific site not provided by mmCIF)	SO4 SULFATE ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；10% Tacsimate, pH 6.0, 10% glycerol, 14-15% PEG 3350, 0.2 mM MgCL2, and 5 mM TCEP	Resolution 3.20 Å R-free 0.284
4PA0 Omecamtiv Mercarbil binding site on the Human Beta-Cardiac Myosin Motor Domain Deposited 2014-04-06	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–787（787 aa） Fragment：UNP P12883 residues 1-787, UNP P42212 residues 5-234	Mutation：Q80R, V163A, I167T, S175G, D190N Non-standard monomer：Yes (specific site not provided by mmCIF)	2OW methyl 4-(2-fluoro-3-{[(6-methylpyridin-3-yl)carbamoyl]amino}benzyl)piperazine-1-carboxylate × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；295 K；Tacsimate, pH 6.0, PEG 3350, glycerol, MgCL2, TCEP and ligand, omecamptiv mercarbil	Resolution 2.25 Å R-free 0.246
4PA0 Omecamtiv Mercarbil binding site on the Human Beta-Cardiac Myosin Motor Domain Deposited 2014-04-06	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric	Chain B 1–787（787 aa） Fragment：UNP P12883 residues 1-787, UNP P42212 residues 5-234	Mutation：Q80R, V163A, I167T, S175G, D190N Non-standard monomer：Yes (specific site not provided by mmCIF)	2OW methyl 4-(2-fluoro-3-{[(6-methylpyridin-3-yl)carbamoyl]amino}benzyl)piperazine-1-carboxylate × 1 GOL GLYCEROL × 3	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；295 K；Tacsimate, pH 6.0, PEG 3350, glycerol, MgCL2, TCEP and ligand, omecamptiv mercarbil	Resolution 2.25 Å R-free 0.246
4XA1 Crystal Structure of the coiled-coil surrounding Skip 1 of MYH7 Deposited 2014-12-12	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 1173–1238（66 aa） Fragment：UNP P13848 residues 1-49,UNP Q12883 residues 1173-1238,UNP Q15691 residues 211-251 Chain B 1173–1238（66 aa） Fragment：UNP P13848 residues 1-49,UNP Q12883 residues 1173-1238,UNP Q15691 residues 211-251	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.6；298 K；20% (w/v) polyethylene glycol methyl ether 2000, 20 mM SrCl2, 100 mM HEPES pH 7.6, 5% pentaerythritol ethoxylate (17/8 PO/OH) 797, 0.5% 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS)	Resolution 3.20 Å R-free 0.285
4XA1 Crystal Structure of the coiled-coil surrounding Skip 1 of MYH7 Deposited 2014-12-12	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 2 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric	Chain C 1173–1238（66 aa） Fragment：UNP P13848 residues 1-49,UNP Q12883 residues 1173-1238,UNP Q15691 residues 211-251 Chain D 1173–1238（66 aa） Fragment：UNP P13848 residues 1-49,UNP Q12883 residues 1173-1238,UNP Q15691 residues 211-251	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.6；298 K；20% (w/v) polyethylene glycol methyl ether 2000, 20 mM SrCl2, 100 mM HEPES pH 7.6, 5% pentaerythritol ethoxylate (17/8 PO/OH) 797, 0.5% 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS)	Resolution 3.20 Å R-free 0.285
4XA3 Crystal structure of the coiled-coil surrounding Skip 2 of MYH7 Deposited 2014-12-12	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 1361–1425（65 aa） Fragment：UNP P13848 residues 1-49,UNP P12883 residues 1361-1425,UNP Q15691 residues 215-251 Chain B 1361–1425（65 aa） Fragment：UNP P13848 residues 1-49,UNP P12883 residues 1361-1425,UNP Q15691 residues 215-251	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5；298 K；4.5% (w/v) polyethylene glycol 8000, 100 mM sodium acetate pH 5.0, 50 mM CaCl2, 2.5% (w/v) 3-methoxy-3-methyl-1-butanol	Resolution 2.55 Å R-free 0.311
4XA4 Crystal Structure of the coiled-coil surrounding Skip 3 of MYH7 Deposited 2014-12-12	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 1551–1609（59 aa） Fragment：UNP Q13426 residues 2-147,UNP P12883 residues 1551-1609 Chain B 1551–1609（59 aa） Fragment：UNP Q13426 residues 2-147,UNP P12883 residues 1551-1609	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；298 K；30% (w/v) polyethylene glycol 1500, 250 mM tetramethylammonium chloride, 100 mM 3-[4-(2-Hydroxyethyl)-1-piperazinyl]propanesulfonic acid (HEPPS)	Resolution 2.33 Å R-free 0.274
4XA6 Crystal Structure of the coiled-coil surrounding Skip 4 of MYH7 Deposited 2014-12-12	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 1777–1855（79 aa） Fragment：UNP P13848 residues 2-50,UNP P02564 residues 1777-1855,UNP Q15691 residues 209-251 Chain B 1777–1855（79 aa） Fragment：UNP P13848 residues 2-50,UNP P02564 residues 1777-1855,UNP Q15691 residues 209-251	Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF)	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.4；298 K；18% (w/v) polyethylene glycol 2000 methyl ether, 100 mM piperazine-N,N-bis(2-ethanesulfonic acid) (PIPES)	Resolution 3.42 Å R-free 0.297
4XA6 Crystal Structure of the coiled-coil surrounding Skip 4 of MYH7 Deposited 2014-12-12	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 2 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric	Chain C 1777–1855（79 aa） Fragment：UNP P13848 residues 2-50,UNP P02564 residues 1777-1855,UNP Q15691 residues 209-251 Chain D 1777–1855（79 aa） Fragment：UNP P13848 residues 2-50,UNP P02564 residues 1777-1855,UNP Q15691 residues 209-251	Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF)	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.4；298 K；18% (w/v) polyethylene glycol 2000 methyl ether, 100 mM piperazine-N,N-bis(2-ethanesulfonic acid) (PIPES)	Resolution 3.42 Å R-free 0.297
5CHX Crystal Structure of amino acids 1590-1657 of MYH7 Deposited 2015-07-10	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 1590–1657（68 aa） Fragment：UNP Q13426 residues 2-143, UNP P12833 1590-1657 Chain B 1590–1657（68 aa） Fragment：UNP Q13426 residues 2-143, UNP P12833 1590-1657	Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF)	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；16% (w/v) MEPEG 2000, 250 mM potassium nitrate, 100 mM 3-(N-morpholino)propanesulfonic acid (MOPS)	Resolution 2.30 Å R-free 0.265
5CHX Crystal Structure of amino acids 1590-1657 of MYH7 Deposited 2015-07-10	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 2 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 1590–1657（68 aa） Fragment：UNP Q13426 residues 2-143, UNP P12833 1590-1657 Chain B 1590–1657（68 aa） Fragment：UNP Q13426 residues 2-143, UNP P12833 1590-1657	Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF)	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；16% (w/v) MEPEG 2000, 250 mM potassium nitrate, 100 mM 3-(N-morpholino)propanesulfonic acid (MOPS)	Resolution 2.30 Å R-free 0.265
5CJ0 Crystal Structure of Amino Acids 1631-1692 of MYH7 Deposited 2015-07-13	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 1631–1692（62 aa） Fragment：UNP Q13426 residues 2-142, UNP P12883 residues 1631-1692 Chain B 1631–1692（62 aa） Fragment：UNP Q13426 residues 2-142, UNP P12883 residues 1631-1692	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；14% (w/v) MEPEG 5000, 200 mM glycine, 100 mM bistrispropane pH 7.0.	Resolution 2.30 Å R-free 0.238
5CJ1 Crystal structure of the coiled coil of MYH7 residues 1526 to 1571 fused to Gp7 Deposited 2015-07-13	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 1526–1571（46 aa） Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571 Chain B 1526–1571（46 aa） Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；16% (w/v) PEG 8000, 400 mM malonate pH 7.2, and 100 mM triethanolamine pH 7.5	Resolution 2.10 Å R-free 0.250
5CJ1 Crystal structure of the coiled coil of MYH7 residues 1526 to 1571 fused to Gp7 Deposited 2015-07-13	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 2 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric	Chain C 1526–1571（46 aa） Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571 Chain D 1526–1571（46 aa） Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；16% (w/v) PEG 8000, 400 mM malonate pH 7.2, and 100 mM triethanolamine pH 7.5	Resolution 2.10 Å R-free 0.250
5CJ1 Crystal structure of the coiled coil of MYH7 residues 1526 to 1571 fused to Gp7 Deposited 2015-07-13	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 3 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric	Chain E 1526–1571（46 aa） Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571 Chain F 1526–1571（46 aa） Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；16% (w/v) PEG 8000, 400 mM malonate pH 7.2, and 100 mM triethanolamine pH 7.5	Resolution 2.10 Å R-free 0.250
5CJ1 Crystal structure of the coiled coil of MYH7 residues 1526 to 1571 fused to Gp7 Deposited 2015-07-13	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 4 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric	Chain G 1526–1571（46 aa） Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571 Chain H 1526–1571（46 aa） Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；16% (w/v) PEG 8000, 400 mM malonate pH 7.2, and 100 mM triethanolamine pH 7.5	Resolution 2.10 Å R-free 0.250
5CJ4 Crystal Structure of Amino Acids 1562-1622 of MYH7 Deposited 2015-07-13	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 1562–1622（61 aa） Fragment：UNP Q13426 residues 2-144, UNP P12883 1562-1622 Chain B 1562–1622（61 aa） Fragment：UNP Q13426 residues 2-144, UNP P12883 1562-1622	Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF)	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；23% (w/v) PEG 4000, 500 mM NaCl, 100 mM triethanolamine pH 8.0.	Resolution 3.10 Å R-free 0.280
5CJ4 Crystal Structure of Amino Acids 1562-1622 of MYH7 Deposited 2015-07-13	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 2 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric	Chain C 1562–1622（61 aa） Fragment：UNP Q13426 residues 2-144, UNP P12883 1562-1622 Chain D 1562–1622（61 aa） Fragment：UNP Q13426 residues 2-144, UNP P12883 1562-1622	Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF)	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；23% (w/v) PEG 4000, 500 mM NaCl, 100 mM triethanolamine pH 8.0.	Resolution 3.10 Å R-free 0.280
5TBY HUMAN BETA CARDIAC HEAVY MEROMYOSIN INTERACTING-HEADS MOTIF OBTAINED BY HOMOLOGY MODELING (USING SWISS-MODEL) OF HUMAN SEQUENCE FROM APHONOPELMA HOMOLOGY MODEL (PDB-3JBH), RIGIDLY FITTED TO HUMAN BETA-CARDIAC NEGATIVELY STAINED THICK FILAMENT 3D-RECONSTRUCTION (EMD-2240) Deposited 2016-09-13	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric	Chain A 1–1935（1935 aa） Fragment：SUBFRAGMENT 1(S1) Chain B 1–1935（1935 aa） Fragment：SUBFRAGMENT 1(S1)	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE；PLUNGING IN A LIQUID ETHANE COOLED BY LIQUID NITROGEN. BLOTTING WAS PERFORMED FROM ONE SIDE OF THE GRID TILL A THIN SAMPLE FILM ON IT USING WHATMAN NO. 42 FILTER PAPER, THEN THE GRID WAS IMMEDIATELY PLUNGED UNDER GRAVITY INTO LIQUID ETHANE COOLED BY LIQUID NITROGEN. GRIDS WERE STORED UNDER LIQUID NITROGEN.	Resolution 20.00 Å
5WJ7 Crystal Structure of Amino Acids 1733-1797 of Human Beta Cardiac Myosin Fused to Xrcc4 Deposited 2017-07-21	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 1733–1797（65 aa） Fragment：UNP Q13426 residues 2-132, UNP P12883 residues 1733-1797 Chain B 1733–1797（65 aa） Fragment：UNP Q13426 residues 2-132, UNP P12883 residues 1733-1797	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；16% (w/v) Methyl-Ether PEG 5K, 300 mM glycine, 100 mM triethanolamine pH 7.5	Resolution 2.50 Å R-free 0.249
5WJB Crystal Structure of Amino Acids 1733-1797 of Human Beta Cardiac Myosin Fused to Gp7 Deposited 2017-07-21	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 1733–1797（65 aa） Fragment：UNP P13848 residues 2-47, UNP P12833 residues 1733-1797 Chain B 1733–1797（65 aa） Fragment：UNP P13848 residues 2-47, UNP P12833 residues 1733-1797	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；14% (w/v) methyl-ether PEG 2K, 1.5%(w/v) myo-inositol, 100 mM HEPES pH 7.5, 50 mM magnesium chloride	Resolution 2.90 Å R-free 0.300
5WJB Crystal Structure of Amino Acids 1733-1797 of Human Beta Cardiac Myosin Fused to Gp7 Deposited 2017-07-21	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 2 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric	Chain C 1733–1797（65 aa） Fragment：UNP P13848 residues 2-47, UNP P12833 residues 1733-1797 Chain D 1733–1797（65 aa） Fragment：UNP P13848 residues 2-47, UNP P12833 residues 1733-1797	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；14% (w/v) methyl-ether PEG 2K, 1.5%(w/v) myo-inositol, 100 mM HEPES pH 7.5, 50 mM magnesium chloride	Resolution 2.90 Å R-free 0.300
5WLQ Crystal Structure of Amino Acids 1677-1755 of Human Beta Cardiac Myosin Fused to Gp7 and Eb1 Deposited 2017-07-27	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 1677–1755（79 aa） Fragment：UNP P13848 residues 2-48 UNP Q15691 residues 208-256, UNP P12883 residues 1677-1755	Not recorded	SO4 SULFATE ION × 2 TMO trimethylamine oxide × 8	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；298 K；1.6 M Ammonium Aulfate, 500 mM Trimethyl Ammonium N-Oxide, 100 mM Bis-tris Propane pH 9.0	Resolution 3.10 Å R-free 0.236
5WLZ Crystal Structure of Amino Acids 1677-1758 of Human Beta Cardiac Myosin Fused to Xrcc4 Deposited 2017-07-28	Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1677–1758（82 aa） Fragment：UNP Q13426 residues 2-132, UNP P12883 residues 1677-1758 Chain B 1677–1758（82 aa） Fragment：UNP Q13426 residues 2-132, UNP P12883 residues 1677-1758 Chain C 1677–1758（82 aa） Fragment：UNP Q13426 residues 2-132, UNP P12883 residues 1677-1758 Chain D 1677–1758（82 aa） Fragment：UNP Q13426 residues 2-132, UNP P12883 residues 1677-1758	Mutation：E29K, E51K, D57A, D58T, E62N, C93R, E98K,C128D, C132A Mutation：E29K, E51K, D57A, D58T, E62N, C93R, E98K,C128D, C132A Mutation：E29K, E51K, D57A, D58T, E62N, C93R, E98K,C128D, C132A Mutation：E29K, E51K, D57A, D58T, E62N, C93R, E98K,C128D, C132A	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；8-10% methyl-ether polyethylene glycol (MEPEG) 5K, 300 mM glycine, bis-tris propane pH 7.0, 1.5-3.0% (w/v) jeffamine M-600	Resolution 3.50 Å R-free 0.249
8ACT structure of the human beta-cardiac myosin folded-back off state Deposited 2022-07-06	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric	Chain A 3–906（904 aa） Chain B 3–906（904 aa）	Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF)	ADP ADENOSINE-5'-DIPHOSPHATE × 2 PO4 PHOSPHATE ION × 2 MG MAGNESIUM ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.60 Å
8EFD Human cardiac myosin II and associated essential light chain in the rigor conformation Deposited 2022-09-08	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain A 1–842（842 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.80 Å
8EFE Human beta-cardiac myosin II bound to ADP-MG2+ and the associated essential light chain Deposited 2022-09-08	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain A 1–842（842 aa）	Not recorded	MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.80 Å
8EFH Helical reconstruction of the human cardiac actin-tropomyosin-myosin complex in complex with ADP-Mg2+ Deposited 2022-09-08	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–842（842 aa）	Not recorded	MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 6	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
8EFI Helical reconstruction of the human cardiac actin-tropomyosin-myosin complex in the rigor form Deposited 2022-09-08	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain M 1–1935（1935 aa）	Not recorded	ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.40 Å
8ENC Helical reconstruction of the human cardiac actin-tropomyosin-myosin loop 4 7G mutant complex Deposited 2022-09-29	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain M 1–1935（1935 aa）	Mutation：Residues 366-372 substituted with seven glycines	ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.60 Å
8G4L Cryo-EM structure of the human cardiac myosin filament Deposited 2023-02-10	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 123 PDB declaration: 123-meric	Chain A 1–1935（1935 aa） Chain AA 1–1935（1935 aa） Chain AB 1–1935（1935 aa） Chain AG 1–1935（1935 aa） Chain AH 1–1935（1935 aa） Chain AI 1–1935（1935 aa） Chain AJ 1–1935（1935 aa） Chain AK 1–1935（1935 aa） Chain AL 1–1935（1935 aa） Chain AM 1–1935（1935 aa） Chain AN 1–1935（1935 aa） Chain AO 1–1935（1935 aa） Chain AP 1–1935（1935 aa） Chain AQ 1–1935（1935 aa） Chain AR 1–1935（1935 aa） Chain AS 1–1935（1935 aa） Chain AT 1–1935（1935 aa） Chain AU 1–1935（1935 aa） Chain AV 1–1935（1935 aa） Chain AW 1–1935（1935 aa） Chain AX 1–1935（1935 aa） Chain AY 1–1935（1935 aa） Chain AZ 1–1935（1935 aa） Chain B 1–1935（1935 aa） Chain BA 1–1935（1935 aa） Chain BB 1–1935（1935 aa） Chain BG 1–1935（1935 aa） Chain BH 1–1935（1935 aa） Chain BI 1–1935（1935 aa） Chain BJ 1–1935（1935 aa） Chain BK 1–1935（1935 aa） Chain BL 1–1935（1935 aa） Chain BM 1–1935（1935 aa） Chain BN 1–1935（1935 aa） Chain BO 1–1935（1935 aa） Chain BP 1–1935（1935 aa） Chain BQ 1–1935（1935 aa） Chain BR 1–1935（1935 aa） Chain BS 1–1935（1935 aa） Chain BT 1–1935（1935 aa） Chain BU 1–1935（1935 aa） Chain BV 1–1935（1935 aa） Chain BW 1–1935（1935 aa） Chain BX 1–1935（1935 aa） Chain BY 1–1935（1935 aa） Chain BZ 1–1935（1935 aa） Chain G 1–1935（1935 aa） Chain H 1–1935（1935 aa） Chain I 1–1935（1935 aa） Chain J 1–1935（1935 aa） Chain K 1–1935（1935 aa） Chain L 1–1935（1935 aa） Chain M 1–1935（1935 aa） Chain N 1–1935（1935 aa） Chain O 1–1935（1935 aa） Chain P 1–1935（1935 aa） Chain Q 1–1935（1935 aa） Chain R 1–1935（1935 aa） Chain S 1–1935（1935 aa） Chain T 1–1935（1935 aa） Chain U 1–1935（1935 aa） Chain V 1–1935（1935 aa） Chain W 1–1935（1935 aa） Chain X 1–1935（1935 aa） Chain Y 1–1935（1935 aa） Chain Z 1–1935（1935 aa） Chain ae 1–1935（1935 aa） Chain af 1–1935（1935 aa） Chain ak 1–1935（1935 aa） Chain al 1–1935（1935 aa） Chain be 1–1935（1935 aa） Chain bf 1–1935（1935 aa） Chain bk 1–1935（1935 aa） Chain bl 1–1935（1935 aa） Chain e 1–1935（1935 aa） Chain f 1–1935（1935 aa） Chain k 1–1935（1935 aa） Chain l 1–1935（1935 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 6.8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 6.40 Å
8ZB7 Human left ventricle ATM complex Deposited 2024-04-26	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric	Chain G 6–781（776 aa） Chain H 6–781（776 aa） Chain I 6–781（776 aa） Chain J 6–781（776 aa） Chain K 6–781（776 aa） Chain M 6–781（776 aa）	Not recorded	ADP ADENOSINE-5'-DIPHOSPHATE × 6	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.19 Å
8ZI9 Human left ventricle actin and myosin complex Deposited 2024-05-13	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain M 6–781（776 aa）	Not recorded	ADP ADENOSINE-5'-DIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.08 Å
9GZ1 Beta-cardiac myosin interacting heads motif complexed to mavacamten Deposited 2024-10-03	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric	Chain A 2–1138（1137 aa） Chain B 2–1138（1137 aa）	Not recorded	MG MAGNESIUM ION × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 2 PO4 PHOSPHATE ION × 2 XB2 Mavacamten × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.2 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
9GZ2 Beta-cardiac heavy meromyosin motor domain in the primed state complexed to mavacamten Deposited 2024-10-03	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 2–1138（1137 aa）	Not recorded	MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1 XB2 Mavacamten × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.2 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
9GZ3 Beta-cardiac heavy meromyosin motor domain in the primed state Deposited 2024-10-03	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 2–1138（1137 aa）	Not recorded	MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.2 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.40 Å
9HTF Beta-cardiac myosin Y115H mutant motor domain in the pre-powerstroke state, MgADP.VO4 form Deposited 2024-12-19	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–810（810 aa）	Non-standard monomer：Yes (specific site not provided by mmCIF)	MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 VO4 VANADATE ION × 1 GOL GLYCEROL × 1 EDO 1,2-ETHANEDIOL × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；23 % PEG 3350 w:v, 0.3 M lithium sulfate, 0.1M Tris-HCl, 2 mM Mg.ADP.Vanadate	Resolution 2.48 Å R-free 0.255
9HTG Beta-cardiac myosin E497D mutant motor domain in the pre-powerstroke state, MgADP.VO4 form Deposited 2024-12-19	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–808（808 aa）	Non-standard monomer：Yes (specific site not provided by mmCIF)	MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 VO4 VANADATE ION × 1 GOL GLYCEROL × 1 EDO 1,2-ETHANEDIOL × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；22 % PEG 3350 w:v, 0.3 M lithium sulfate, 0.1M Tris-HCl, 2 mM Mg.ADP.Vanadate	Resolution 2.60 Å R-free 0.248
9I8P Human beta-cardiac myosin wild type motor domain in the pre-powerstroke state, MgADP.VO4 form Deposited 2025-02-05	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–808（808 aa）	Non-standard monomer：Yes (specific site not provided by mmCIF)	MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 VO4 VANADATE ION × 1 EDO 1,2-ETHANEDIOL × 2 SO4 SULFATE ION × 2 GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；25 % PEG 3350 w:v, 0.25 M lithium sulfate, 0.1M Tris-HCl, 2 mM Mg.ADP.Vanadate. Optimal crystals were obtained using the micro-seeding technique.	Resolution 2.60 Å R-free 0.245
9YOP Cryo-EM structure of human beta-cardiac myosin in the interacting-heads motif and S2-FH docked state Deposited 2025-10-13	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric	Chain A 1–1016（1016 aa） Chain B 1–1016（1016 aa）	Not recorded	ADP ADENOSINE-5'-DIPHOSPHATE × 2 PO4 PHOSPHATE ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.50 Å
9YP4 Cryo-EM structure of human beta-cardiac myosin bound to omecamtiv mecarbil in the interacting-heads motif and S2-FH docked state Deposited 2025-10-13	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric	Chain A 1–1016（1016 aa） Chain B 1–1016（1016 aa）	Not recorded	2OW methyl 4-(2-fluoro-3-{[(6-methylpyridin-3-yl)carbamoyl]amino}benzyl)piperazine-1-carboxylate × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 PO4 PHOSPHATE ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.50 Å
9YP9 Cryo-EM structure of human beta-cardiac myosin bound to mavacamten in the interacting-heads motif and S2-FH docked state Deposited 2025-10-13	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric	Chain A 1–1016（1016 aa） Chain B 1–1016（1016 aa）	Not recorded	PO4 PHOSPHATE ION × 2 XB2 Mavacamten × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
9YR7 Cryo-EM structure of human beta-cardiac myosin bound to mavacamten in the interacting-heads motif and S2-FH undocked state Deposited 2025-10-16	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric	Chain A 1–1016（1016 aa） Chain B 1–1016（1016 aa）	Not recorded	XB2 Mavacamten × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 PO4 PHOSPHATE ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
9YRG Cryo-EM structure of human beta-cardiac myosin in the interacting-heads motif and S2-FH undocked state Deposited 2025-10-16	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric	Chain A 1–1016（1016 aa） Chain B 1–1016（1016 aa）	Not recorded	ADP ADENOSINE-5'-DIPHOSPHATE × 2 PO4 PHOSPHATE ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
9YRH Cryo-EM structure of human beta-cardiac myosin bound to omecamtiv mecarbil in the interacting-heads motif and S2-FH undocked state Deposited 2025-10-16	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric	Chain A 1–1016（1016 aa） Chain B 1–1016（1016 aa）	Not recorded	2OW methyl 4-(2-fluoro-3-{[(6-methylpyridin-3-yl)carbamoyl]amino}benzyl)piperazine-1-carboxylate × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 PO4 PHOSPHATE ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.80 Å

40 other PDB entries and 55 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	MYH7_HUMAN
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 51–109； UniProt 1728–1786 Author chain B； PDBConstruct 51–109； UniProt 1728–1786 Author chain C； PDBConstruct 51–109； UniProt 1728–1786 Author chain D； PDBConstruct 51–109； UniProt 1728–1786

Capsid assembly scaffolding protein,Myosin-7

Homo sapiens

UniProt P13848

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Insufficient information Homooligomer Protein × 4 PDB declaration: tetrameric（4） Consistent with protein copy count	Chain A； UniProt 2–47 Chain B； UniProt 2–47 Chain C； UniProt 2–47 Chain D； UniProt 2–47	Fragment：UNP P13848 residues 2-48, UNP P12883 residues 1729-1786	No other associated polymer	X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 5；298 K；18% (w/v) pentaerythritol ethoxylate 797, 150 mM ammonium thiocyanate, 100 mM sodium acetate pH 5.0.	Resolution 2.30 Å R-free 0.264

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 5WME	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
1NO4 Crystal Structure of the pre-assembly scaffolding protein gp7 from the double-stranded DNA bacteriophage phi29 Deposited 2003-01-15	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 1–97（97 aa） Chain B 1–97（97 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；4 % PEG 8000, 0.16 M calcium acetate, 0.08 M sodium cacodylate, 20 % glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 2.20 Å R-free 0.262
1NO4 Crystal Structure of the pre-assembly scaffolding protein gp7 from the double-stranded DNA bacteriophage phi29 Deposited 2003-01-15	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain C 1–97（97 aa） Chain D 1–97（97 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；4 % PEG 8000, 0.16 M calcium acetate, 0.08 M sodium cacodylate, 20 % glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 2.20 Å R-free 0.262
1NO4 Crystal Structure of the pre-assembly scaffolding protein gp7 from the double-stranded DNA bacteriophage phi29 Deposited 2003-01-15	Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 3 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–97（97 aa） Chain B 1–97（97 aa） Chain C 1–97（97 aa） Chain D 1–97（97 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；4 % PEG 8000, 0.16 M calcium acetate, 0.08 M sodium cacodylate, 20 % glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 2.20 Å R-free 0.262
1NOH The structure of bacteriophage phi29 scaffolding protein gp7 after prohead assembly Deposited 2003-01-16	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 1–97（97 aa） Chain B 1–97（97 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；14.4 % Peg 8000, 0.08 M sodium cacodylate, 0.16 M calcium acetate, 20 % glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 2.80 Å R-free 0.290
1NOH The structure of bacteriophage phi29 scaffolding protein gp7 after prohead assembly Deposited 2003-01-16	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain C 1–97（97 aa） Chain D 1–97（97 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；14.4 % Peg 8000, 0.08 M sodium cacodylate, 0.16 M calcium acetate, 20 % glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 2.80 Å R-free 0.290
1NOH The structure of bacteriophage phi29 scaffolding protein gp7 after prohead assembly Deposited 2003-01-16	Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 3 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–97（97 aa） Chain B 1–97（97 aa） Chain C 1–97（97 aa） Chain D 1–97（97 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；14.4 % Peg 8000, 0.08 M sodium cacodylate, 0.16 M calcium acetate, 20 % glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 2.80 Å R-free 0.290
3MTU Structure of the Tropomyosin Overlap Complex from Chicken Smooth Muscle Deposited 2010-04-30	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric	Chain E 2–46（45 aa） Fragment：;Fusion protein of residues 2-45 of phage phi29 Gp7 protein and residues 256-284 of chicken smooth muscle tropomyosin,Fusion protein of residues 2-45 of phage phi29 Gp7 protein and residues 256-284 of chicken smooth muscle tropomyosin ; Chain F 2–46（45 aa） Fragment：;Fusion protein of residues 2-45 of phage phi29 Gp7 protein and residues 256-284 of chicken smooth muscle tropomyosin,Fusion protein of residues 2-45 of phage phi29 Gp7 protein and residues 256-284 of chicken smooth muscle tropomyosin ;	Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF)	CL CHLORIDE ION × 2 EDO 1,2-ETHANEDIOL × 9 EOH ETHANOL × 7	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；16% MePEG 5000, 100 mM MES, pH 6.0, 140 mM CaCl2, 2% methanol , VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 2.10 Å R-free 0.241
3MTU Structure of the Tropomyosin Overlap Complex from Chicken Smooth Muscle Deposited 2010-04-30	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Insufficient information Heteromer；Protein × 5 PDB declaration: pentameric	Chain F 2–46（45 aa） Fragment：;Fusion protein of residues 2-45 of phage phi29 Gp7 protein and residues 256-284 of chicken smooth muscle tropomyosin,Fusion protein of residues 2-45 of phage phi29 Gp7 protein and residues 256-284 of chicken smooth muscle tropomyosin ;	Non-standard monomer：Yes (specific site not provided by mmCIF)	CL CHLORIDE ION × 2 EDO 1,2-ETHANEDIOL × 9 EOH ETHANOL × 7	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；16% MePEG 5000, 100 mM MES, pH 6.0, 140 mM CaCl2, 2% methanol , VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 2.10 Å R-free 0.241
3OA7 Structure of the C-terminal domain of Cnm67, a core component of the spindle pole body of Saccharomyces cerevisiae Deposited 2010-08-04	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 2–50（49 aa）	Non-standard monomer：Yes (specific site not provided by mmCIF)	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；293 K；10% monomethyl PEG 5000, 1.0M tetramethyl ammonium chloride, 100 mM CHES, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 2.30 Å R-free 0.271
4IFF Structural organization of FtsB, a transmembrane protein of the bacterial divisome Deposited 2012-12-14	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 2–48（47 aa） Fragment：UNP P13848 residues 2-48, UNP P0A6S5 residues 28-63 Chain B 2–48（47 aa） Fragment：UNP P13848 residues 2-48, UNP P0A6S5 residues 28-63	Not recorded	GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；290 K；2.1 M ammonium sulfate, 0.6 M malonate, 5% glycerol, 100 mM Bis-Tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 290 K	Resolution 2.30 Å R-free 0.256
4IFF Structural organization of FtsB, a transmembrane protein of the bacterial divisome Deposited 2012-12-14	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric	Chain C 2–48（47 aa） Fragment：UNP P13848 residues 2-48, UNP P0A6S5 residues 28-63 Chain D 2–48（47 aa） Fragment：UNP P13848 residues 2-48, UNP P0A6S5 residues 28-63	Not recorded	GOL GLYCEROL × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；290 K；2.1 M ammonium sulfate, 0.6 M malonate, 5% glycerol, 100 mM Bis-Tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 290 K	Resolution 2.30 Å R-free 0.256
4XA1 Crystal Structure of the coiled-coil surrounding Skip 1 of MYH7 Deposited 2014-12-12	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 1–49（49 aa） Fragment：UNP P13848 residues 1-49,UNP Q12883 residues 1173-1238,UNP Q15691 residues 211-251 Chain B 1–49（49 aa） Fragment：UNP P13848 residues 1-49,UNP Q12883 residues 1173-1238,UNP Q15691 residues 211-251	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.6；298 K；20% (w/v) polyethylene glycol methyl ether 2000, 20 mM SrCl2, 100 mM HEPES pH 7.6, 5% pentaerythritol ethoxylate (17/8 PO/OH) 797, 0.5% 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS)	Resolution 3.20 Å R-free 0.285
4XA1 Crystal Structure of the coiled-coil surrounding Skip 1 of MYH7 Deposited 2014-12-12	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 2 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric	Chain C 1–49（49 aa） Fragment：UNP P13848 residues 1-49,UNP Q12883 residues 1173-1238,UNP Q15691 residues 211-251 Chain D 1–49（49 aa） Fragment：UNP P13848 residues 1-49,UNP Q12883 residues 1173-1238,UNP Q15691 residues 211-251	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.6；298 K；20% (w/v) polyethylene glycol methyl ether 2000, 20 mM SrCl2, 100 mM HEPES pH 7.6, 5% pentaerythritol ethoxylate (17/8 PO/OH) 797, 0.5% 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS)	Resolution 3.20 Å R-free 0.285
4XA3 Crystal structure of the coiled-coil surrounding Skip 2 of MYH7 Deposited 2014-12-12	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 1–49（49 aa） Fragment：UNP P13848 residues 1-49,UNP P12883 residues 1361-1425,UNP Q15691 residues 215-251 Chain B 1–49（49 aa） Fragment：UNP P13848 residues 1-49,UNP P12883 residues 1361-1425,UNP Q15691 residues 215-251	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5；298 K；4.5% (w/v) polyethylene glycol 8000, 100 mM sodium acetate pH 5.0, 50 mM CaCl2, 2.5% (w/v) 3-methoxy-3-methyl-1-butanol	Resolution 2.55 Å R-free 0.311
4XA6 Crystal Structure of the coiled-coil surrounding Skip 4 of MYH7 Deposited 2014-12-12	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 2–50（49 aa） Fragment：UNP P13848 residues 2-50,UNP P02564 residues 1777-1855,UNP Q15691 residues 209-251 Chain B 2–50（49 aa） Fragment：UNP P13848 residues 2-50,UNP P02564 residues 1777-1855,UNP Q15691 residues 209-251	Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF)	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.4；298 K；18% (w/v) polyethylene glycol 2000 methyl ether, 100 mM piperazine-N,N-bis(2-ethanesulfonic acid) (PIPES)	Resolution 3.42 Å R-free 0.297
4XA6 Crystal Structure of the coiled-coil surrounding Skip 4 of MYH7 Deposited 2014-12-12	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 2 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric	Chain C 2–50（49 aa） Fragment：UNP P13848 residues 2-50,UNP P02564 residues 1777-1855,UNP Q15691 residues 209-251 Chain D 2–50（49 aa） Fragment：UNP P13848 residues 2-50,UNP P02564 residues 1777-1855,UNP Q15691 residues 209-251	Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF)	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.4；298 K；18% (w/v) polyethylene glycol 2000 methyl ether, 100 mM piperazine-N,N-bis(2-ethanesulfonic acid) (PIPES)	Resolution 3.42 Å R-free 0.297
5CJ1 Crystal structure of the coiled coil of MYH7 residues 1526 to 1571 fused to Gp7 Deposited 2015-07-13	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 2–52（51 aa） Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571 Chain B 2–52（51 aa） Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；16% (w/v) PEG 8000, 400 mM malonate pH 7.2, and 100 mM triethanolamine pH 7.5	Resolution 2.10 Å R-free 0.250
5CJ1 Crystal structure of the coiled coil of MYH7 residues 1526 to 1571 fused to Gp7 Deposited 2015-07-13	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 2 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric	Chain C 2–52（51 aa） Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571 Chain D 2–52（51 aa） Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；16% (w/v) PEG 8000, 400 mM malonate pH 7.2, and 100 mM triethanolamine pH 7.5	Resolution 2.10 Å R-free 0.250
5CJ1 Crystal structure of the coiled coil of MYH7 residues 1526 to 1571 fused to Gp7 Deposited 2015-07-13	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 3 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric	Chain E 2–52（51 aa） Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571 Chain F 2–52（51 aa） Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；16% (w/v) PEG 8000, 400 mM malonate pH 7.2, and 100 mM triethanolamine pH 7.5	Resolution 2.10 Å R-free 0.250
5CJ1 Crystal structure of the coiled coil of MYH7 residues 1526 to 1571 fused to Gp7 Deposited 2015-07-13	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 4 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric	Chain G 2–52（51 aa） Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571 Chain H 2–52（51 aa） Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；16% (w/v) PEG 8000, 400 mM malonate pH 7.2, and 100 mM triethanolamine pH 7.5	Resolution 2.10 Å R-free 0.250
5WJB Crystal Structure of Amino Acids 1733-1797 of Human Beta Cardiac Myosin Fused to Gp7 Deposited 2017-07-21	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 2–48（47 aa） Fragment：UNP P13848 residues 2-47, UNP P12833 residues 1733-1797 Chain B 2–48（47 aa） Fragment：UNP P13848 residues 2-47, UNP P12833 residues 1733-1797	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；14% (w/v) methyl-ether PEG 2K, 1.5%(w/v) myo-inositol, 100 mM HEPES pH 7.5, 50 mM magnesium chloride	Resolution 2.90 Å R-free 0.300
5WJB Crystal Structure of Amino Acids 1733-1797 of Human Beta Cardiac Myosin Fused to Gp7 Deposited 2017-07-21	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 2 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric	Chain C 2–48（47 aa） Fragment：UNP P13848 residues 2-47, UNP P12833 residues 1733-1797 Chain D 2–48（47 aa） Fragment：UNP P13848 residues 2-47, UNP P12833 residues 1733-1797	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；14% (w/v) methyl-ether PEG 2K, 1.5%(w/v) myo-inositol, 100 mM HEPES pH 7.5, 50 mM magnesium chloride	Resolution 2.90 Å R-free 0.300
5WLQ Crystal Structure of Amino Acids 1677-1755 of Human Beta Cardiac Myosin Fused to Gp7 and Eb1 Deposited 2017-07-27	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 2–48（47 aa） Fragment：UNP P13848 residues 2-48 UNP Q15691 residues 208-256, UNP P12883 residues 1677-1755	Not recorded	SO4 SULFATE ION × 2 TMO trimethylamine oxide × 8	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；298 K；1.6 M Ammonium Aulfate, 500 mM Trimethyl Ammonium N-Oxide, 100 mM Bis-tris Propane pH 9.0	Resolution 3.10 Å R-free 0.236
6YJD Lamin A coil2 dimer stabilized by N-terminal capping Deposited 2020-04-03	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 1–48（48 aa）	Mutation：F40C	NI NICKEL (II) ION × 4 CL CHLORIDE ION × 2 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 4	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.2；277 K；35% (v/v) methanol, 0.2 M MgCl2 and 0.1 M HEPES	Resolution 2.90 Å R-free 0.304
8F2M Phi-29 scaffolding protein bound to intermediate-state MCP Deposited 2022-11-08	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric	Chain B 1–98（98 aa） Chain C 1–98（98 aa） Chain F 1–98（98 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å

13 other PDB entries and 25 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	SCAF_BPPH2
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 5–50； UniProt 2–47 Author chain B； PDBConstruct 5–50； UniProt 2–47 Author chain C； PDBConstruct 5–50； UniProt 2–47 Author chain D； PDBConstruct 5–50； UniProt 2–47

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

P(r) Distance Distribution P(r) Distribution

Download Download SAXS (.dat) P(r) (.pr.out) CIF

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	5wme
Deposition date deposition_date	2017-07-28
Structure title title	Crystal Structure of Amino Acids 1729-1786 of Human Beta Cardiac Myosin Fused to Gp7 as Anti-Parallel Four-Helix Bundle
Keywords keywords	Myosin, Gp7, Coiled-Coil, MOTOR PROTEIN; MOTOR PROTEIN
Experimental Method method	X-RAY DIFFRACTION

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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier	40.99 Å
Radius of gyration Rg (electron density) rg_electron	43.04 Å
Forward intensity I(0) i0	30119900.00
Molecular weight molecular_weight	41558.0 kDa
Excluded volume excluded_volume	51250 Å³
Envelope volume envelope_volume	70592 Å³
Hydration-shell volume shell_volume	18572 Å³
Envelope diameter envelope_diameter	156.3 Å
Shell Rg shell_rg	33.88 Å
Envelope Rg envelope_rg	43.80 Å
Shape Rg shape_rg	43.17 Å
Total Rg total_rg	42.01 Å
Total atoms total_atoms	2906
Residues n_residues	365
Spherical-harmonic order n_harmonics	20
q range q_range	— – 0.5000 Å⁻¹
Data points n_points	101
Shell type shell_type	directional
Solvent electron density solvent_density	0.3340 e/Å³
Shell contrast contrast_shell	0.0300 e/Å³
CRYSOL version crysol_version	4.1.3

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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax	148.2 Å
Rg (real space) rg_real	42.07 Å
Rg uncertainty (real space) rg_real_error	2.51 Å
I(0) (real space) i0_real	3.0120e+07
I(0) uncertainty (real space) i0_real_error	6.0240e+05
Rg (reciprocal space) rg_reciprocal	41.00 Å
I(0) (reciprocal space) i0_reciprocal	30090000.0000
Solution quality estimate total_estimate	0.5639
Solution quality rating solution_quality	REASONABLE a REASONABLE solution
P(r) peaks n_peaks	3
Primary peak position r_peak_primary	19.0 Å
Skewness Skewness skewness	0.721
Kurtosis Kurtosis kurtosis	-0.283
Angular range angular_range	— – 0.1950 Å⁻¹
Current regularization parameter α current_alpha	0.0000
Highest regularization parameter α highest_alpha	759800.0000
Real-space data points n_real_points	40
GNOM version gnom_version	4.1.3
Quality Criteria quality_criteria	AN1: 0.000; Oscil: 0.051; Stabil: 0.999; Sysdev: 1.000; Positv: 1.000; Valcen: 0.008; Smooth: 0.169

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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (2)

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8. Citations (1)

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Unit-cell length a length_a	24.247 Å
Unit-cell length b length_b	72.960 Å
Unit-cell length c length_c	114.484 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	96.29 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	8

Space group space_group	P 1 21 1
Space-group number int_tables_number	4
Crystal system crystal_system	—

Resolution resolution	2.300 Å
Low-resolution limit resolution_low	44.867 Å
R factor r_factor	0.2262
R_work r_work	0.2242
R_free r_free	0.2643

Description description	Capsid assembly scaffolding protein,Myosin-7
Molecular weight formula_weight	12354.8 kDa
Source method src_method	man
Copy count entity_count	4

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B,C,D
Atom count atom_count	2957

Crystal Structure of Amino Acids 1729-1786 of Human Beta Cardiac Myosin Fused to Gp7 as Anti-Parallel Four-Helix Bundle

1. Protein Identity and Related Structures Protein Identity & Related Structures

Capsid assembly scaffolding protein,Myosin-7

State in the Current Structure

Other States of the Same Protein in the Database

Capsid assembly scaffolding protein,Myosin-7

State in the Current Structure

Other States of the Same Protein in the Database

SAXS scattering curve SAXS Profile

P(r) Distance Distribution P(r) Distribution

2. Structure Basics 2. Structure Basics

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

5. Crystallography and Experiment 5. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

6. Entities and Polymers Entities & Polymers (2)

Entity 1 Entity 1 polymer

Polymer Polymer

来源 Source

Entity 2 Entity 2 water

8. Citations (1)

9. Files and Curves (10)

Files (7)

Curves (3)

来源生物 organism	Homo sapiens
通用名 common_name	Human
NCBI Class编号 ncbi_taxonomy_id	9606
宿主生物 host_organism	Escherichia coli

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	51

fit	/app/data/saxs_computation/wm/5wme/5wme.fit
pddf	/app/data/pr_computation/wm/5wme/5wme.pr.out
profile	/app/data/saxs_computation/wm/5wme/5wme.dat