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4xa6

Crystal Structure of the coiled-coil surrounding Skip 4 of MYH7

Method: X-RAY DIFFRACTION Dmax: 207.0 Å Quality: REASONABLE
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Gp7-MYH7(1777-1855)-EB1 chimera protein

Homo sapiens

UniProt P12883

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Insufficient information Homooligomer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count Chain A； UniProt 1777–1855 Chain B； UniProt 1777–1855 Fragment：UNP P13848 residues 2-50,UNP P02564 residues 1777-1855,UNP Q15691 residues 209-251 Non-standard monomer：Yes (specific site not provided by mmCIF) No other associated polymer X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 6.4；298 K；18% (w/v) polyethylene glycol 2000 methyl ether, 100 mM piperazine-N,N-bis(2-ethanesulfonic acid) (PIPES) Resolution 3.42 Å R-free 0.297
2 Insufficient information Homooligomer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count Chain C； UniProt 1777–1855 Chain D； UniProt 1777–1855 Fragment：UNP P13848 residues 2-50,UNP P02564 residues 1777-1855,UNP Q15691 residues 209-251 Non-standard monomer：Yes (specific site not provided by mmCIF) No other associated polymer X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 6.4；298 K；18% (w/v) polyethylene glycol 2000 methyl ether, 100 mM piperazine-N,N-bis(2-ethanesulfonic acid) (PIPES) Resolution 3.42 Å R-free 0.297

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4XA6 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
2FXM Structure of the human beta-myosin S2 fragment Deposited 2006-02-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 838–963（126 aa） Fragment：DELTA-S2 FRAGMENT (838-963)
Chain B 838–963（126 aa） Fragment：DELTA-S2 FRAGMENT (838-963)
Not recorded HG MERCURY (II) ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；296 K；PEG 3350, LITHIUM CITRATE, TRIS- HCL, pH 8.50, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 296K
Resolution 2.70 Å R-free 0.283
2FXO Structure of the human beta-myosin S2 fragment Deposited 2006-02-06 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 838–963（126 aa） Fragment：DELTA-S2 FRAGMENT (838-963)
Chain B 838–963（126 aa） Fragment：DELTA-S2 FRAGMENT (838-963)
Mutation：E924K Mutation：E924K No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；296 K；PEG 3350, SODIUM ACETATE, TRIS-HCL, pH 7.50, VAPOR DIFFUSION, SITTING DROP, temperature 296K
Resolution 2.50 Å R-free 0.349
2FXO Structure of the human beta-myosin S2 fragment Deposited 2006-02-06 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 838–963（126 aa） Fragment：DELTA-S2 FRAGMENT (838-963)
Chain D 838–963（126 aa） Fragment：DELTA-S2 FRAGMENT (838-963)
Mutation：E924K Mutation：E924K No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；296 K；PEG 3350, SODIUM ACETATE, TRIS-HCL, pH 7.50, VAPOR DIFFUSION, SITTING DROP, temperature 296K
Resolution 2.50 Å R-free 0.349
3DTP Tarantula heavy meromyosin obtained by flexible docking to Tarantula muscle thick filament Cryo-EM 3D-MAP Deposited 2008-07-15 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 216 PDB declaration: 216-meric
Chain A 842–961（120 aa） Fragment：SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-972),SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-972)
Chain B 842–963（122 aa） Fragment：SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-974),SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-974)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 100mM NaCl, 3mM MgCl2, 1mM EGTA, 5mM PIPES, 5mM NaH2PO4, 1mM NaN3；pH 7；100mM NaCl, 3mM MgCl2, 1mM EGTA, 5mM PIPES, 5mM NaH2PO4, 1mM NaN3
cryo-EM vitrification conditions Plunging in a liquid ethane. Blotting was performed from one side of the grid till a thin sample film on it using Whatman No 42 filter paper, then the grid was immediately plunged under gravity into liquid ethane cooled by liquid nitrogen. Grids were stored under liquid nitrogen.
Resolution 20.00 Å
3DTP Tarantula heavy meromyosin obtained by flexible docking to Tarantula muscle thick filament Cryo-EM 3D-MAP Deposited 2008-07-15 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 842–961（120 aa） Fragment：SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-972),SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-972)
Chain B 842–963（122 aa） Fragment：SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-974),SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-974)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 100mM NaCl, 3mM MgCl2, 1mM EGTA, 5mM PIPES, 5mM NaH2PO4, 1mM NaN3；pH 7；100mM NaCl, 3mM MgCl2, 1mM EGTA, 5mM PIPES, 5mM NaH2PO4, 1mM NaN3
cryo-EM vitrification conditions Plunging in a liquid ethane. Blotting was performed from one side of the grid till a thin sample film on it using Whatman No 42 filter paper, then the grid was immediately plunged under gravity into liquid ethane cooled by liquid nitrogen. Grids were stored under liquid nitrogen.
Resolution 20.00 Å
3DTP Tarantula heavy meromyosin obtained by flexible docking to Tarantula muscle thick filament Cryo-EM 3D-MAP Deposited 2008-07-15 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 842–961（120 aa） Fragment：SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-972),SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-972)
Chain B 842–963（122 aa） Fragment：SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-974),SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-974)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 100mM NaCl, 3mM MgCl2, 1mM EGTA, 5mM PIPES, 5mM NaH2PO4, 1mM NaN3；pH 7；100mM NaCl, 3mM MgCl2, 1mM EGTA, 5mM PIPES, 5mM NaH2PO4, 1mM NaN3
cryo-EM vitrification conditions Plunging in a liquid ethane. Blotting was performed from one side of the grid till a thin sample film on it using Whatman No 42 filter paper, then the grid was immediately plunged under gravity into liquid ethane cooled by liquid nitrogen. Grids were stored under liquid nitrogen.
Resolution 20.00 Å
4DB1 Cardiac human myosin S1dC, beta isoform complexed with Mn-AMPPNP Deposited 2012-01-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 2–783（782 aa） Fragment：UNP residues 2-783
Not recorded ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 MN MANGANESE (II) ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；277 K；precipitant: 7.5% PEG 8000, 0.05M MES, 0.05M acetate, 0.25M sodium chloride, 0.01M manganese chloride, 0.1% sodium cholate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.60 Å R-free 0.257
4DB1 Cardiac human myosin S1dC, beta isoform complexed with Mn-AMPPNP Deposited 2012-01-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 2–783（782 aa） Fragment：UNP residues 2-783
Not recorded ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 MN MANGANESE (II) ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；277 K；precipitant: 7.5% PEG 8000, 0.05M MES, 0.05M acetate, 0.25M sodium chloride, 0.01M manganese chloride, 0.1% sodium cholate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.60 Å R-free 0.257
4DB1 Cardiac human myosin S1dC, beta isoform complexed with Mn-AMPPNP Deposited 2012-01-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 2–783（782 aa） Fragment：UNP residues 2-783
Chain B 2–783（782 aa） Fragment：UNP residues 2-783
Not recorded ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 MN MANGANESE (II) ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；277 K；precipitant: 7.5% PEG 8000, 0.05M MES, 0.05M acetate, 0.25M sodium chloride, 0.01M manganese chloride, 0.1% sodium cholate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.60 Å R-free 0.257
4P7H Structure of Human beta-Cardiac Myosin Motor Domain::GFP chimera Deposited 2014-03-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–787（787 aa） Fragment：UNP P12883 residues 1-787,UNP P42212 residues 5-238
Mutation：Q80R, K101N, V163A, I167T, S175G, D190N Non-standard monomer：Yes (specific site not provided by mmCIF) SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；10% Tacsimate, pH 6.0, 10% glycerol, 14-15% PEG 3350, 0.2 mM MgCL2, and 5 mM TCEP
Resolution 3.20 Å R-free 0.284
4P7H Structure of Human beta-Cardiac Myosin Motor Domain::GFP chimera Deposited 2014-03-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1–787（787 aa） Fragment：UNP P12883 residues 1-787,UNP P42212 residues 5-238
Mutation：Q80R, K101N, V163A, I167T, S175G, D190N Non-standard monomer：Yes (specific site not provided by mmCIF) SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；10% Tacsimate, pH 6.0, 10% glycerol, 14-15% PEG 3350, 0.2 mM MgCL2, and 5 mM TCEP
Resolution 3.20 Å R-free 0.284
4PA0 Omecamtiv Mercarbil binding site on the Human Beta-Cardiac Myosin Motor Domain Deposited 2014-04-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–787（787 aa） Fragment：UNP P12883 residues 1-787, UNP P42212 residues 5-234
Mutation：Q80R, V163A, I167T, S175G, D190N Non-standard monomer：Yes (specific site not provided by mmCIF) 2OW methyl 4-(2-fluoro-3-{[(6-methylpyridin-3-yl)carbamoyl]amino}benzyl)piperazine-1-carboxylate × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；295 K；Tacsimate, pH 6.0, PEG 3350, glycerol, MgCL2, TCEP and ligand, omecamptiv mercarbil
Resolution 2.25 Å R-free 0.246
4PA0 Omecamtiv Mercarbil binding site on the Human Beta-Cardiac Myosin Motor Domain Deposited 2014-04-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1–787（787 aa） Fragment：UNP P12883 residues 1-787, UNP P42212 residues 5-234
Mutation：Q80R, V163A, I167T, S175G, D190N Non-standard monomer：Yes (specific site not provided by mmCIF) 2OW methyl 4-(2-fluoro-3-{[(6-methylpyridin-3-yl)carbamoyl]amino}benzyl)piperazine-1-carboxylate × 1 GOL GLYCEROL × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；295 K；Tacsimate, pH 6.0, PEG 3350, glycerol, MgCL2, TCEP and ligand, omecamptiv mercarbil
Resolution 2.25 Å R-free 0.246
4XA1 Crystal Structure of the coiled-coil surrounding Skip 1 of MYH7 Deposited 2014-12-12 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1173–1238（66 aa） Fragment：UNP P13848 residues 1-49,UNP Q12883 residues 1173-1238,UNP Q15691 residues 211-251
Chain B 1173–1238（66 aa） Fragment：UNP P13848 residues 1-49,UNP Q12883 residues 1173-1238,UNP Q15691 residues 211-251
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.6；298 K；20% (w/v) polyethylene glycol methyl ether 2000, 20 mM SrCl2, 100 mM HEPES pH 7.6, 5% pentaerythritol ethoxylate (17/8 PO/OH) 797, 0.5% 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS)
Resolution 3.20 Å R-free 0.285
4XA1 Crystal Structure of the coiled-coil surrounding Skip 1 of MYH7 Deposited 2014-12-12 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 1173–1238（66 aa） Fragment：UNP P13848 residues 1-49,UNP Q12883 residues 1173-1238,UNP Q15691 residues 211-251
Chain D 1173–1238（66 aa） Fragment：UNP P13848 residues 1-49,UNP Q12883 residues 1173-1238,UNP Q15691 residues 211-251
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.6；298 K；20% (w/v) polyethylene glycol methyl ether 2000, 20 mM SrCl2, 100 mM HEPES pH 7.6, 5% pentaerythritol ethoxylate (17/8 PO/OH) 797, 0.5% 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS)
Resolution 3.20 Å R-free 0.285
4XA3 Crystal structure of the coiled-coil surrounding Skip 2 of MYH7 Deposited 2014-12-12 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1361–1425（65 aa） Fragment：UNP P13848 residues 1-49,UNP P12883 residues 1361-1425,UNP Q15691 residues 215-251
Chain B 1361–1425（65 aa） Fragment：UNP P13848 residues 1-49,UNP P12883 residues 1361-1425,UNP Q15691 residues 215-251
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5；298 K；4.5% (w/v) polyethylene glycol 8000, 100 mM sodium acetate pH 5.0, 50 mM CaCl2, 2.5% (w/v) 3-methoxy-3-methyl-1-butanol
Resolution 2.55 Å R-free 0.311
4XA4 Crystal Structure of the coiled-coil surrounding Skip 3 of MYH7 Deposited 2014-12-12 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1551–1609（59 aa） Fragment：UNP Q13426 residues 2-147,UNP P12883 residues 1551-1609
Chain B 1551–1609（59 aa） Fragment：UNP Q13426 residues 2-147,UNP P12883 residues 1551-1609
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；298 K；30% (w/v) polyethylene glycol 1500, 250 mM tetramethylammonium chloride, 100 mM 3-[4-(2-Hydroxyethyl)-1-piperazinyl]propanesulfonic acid (HEPPS)
Resolution 2.33 Å R-free 0.274
5CHX Crystal Structure of amino acids 1590-1657 of MYH7 Deposited 2015-07-10 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1590–1657（68 aa） Fragment：UNP Q13426 residues 2-143, UNP P12833 1590-1657
Chain B 1590–1657（68 aa） Fragment：UNP Q13426 residues 2-143, UNP P12833 1590-1657
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；16% (w/v) MEPEG 2000, 250 mM potassium nitrate, 100 mM 3-(N-morpholino)propanesulfonic acid (MOPS)
Resolution 2.30 Å R-free 0.265
5CHX Crystal Structure of amino acids 1590-1657 of MYH7 Deposited 2015-07-10 Different construct Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1590–1657（68 aa） Fragment：UNP Q13426 residues 2-143, UNP P12833 1590-1657
Chain B 1590–1657（68 aa） Fragment：UNP Q13426 residues 2-143, UNP P12833 1590-1657
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；16% (w/v) MEPEG 2000, 250 mM potassium nitrate, 100 mM 3-(N-morpholino)propanesulfonic acid (MOPS)
Resolution 2.30 Å R-free 0.265
5CJ0 Crystal Structure of Amino Acids 1631-1692 of MYH7 Deposited 2015-07-13 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1631–1692（62 aa） Fragment：UNP Q13426 residues 2-142, UNP P12883 residues 1631-1692
Chain B 1631–1692（62 aa） Fragment：UNP Q13426 residues 2-142, UNP P12883 residues 1631-1692
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；14% (w/v) MEPEG 5000, 200 mM glycine, 100 mM bistrispropane pH 7.0.
Resolution 2.30 Å R-free 0.238
5CJ1 Crystal structure of the coiled coil of MYH7 residues 1526 to 1571 fused to Gp7 Deposited 2015-07-13 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1526–1571（46 aa） Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571
Chain B 1526–1571（46 aa） Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；16% (w/v) PEG 8000, 400 mM malonate pH 7.2, and 100 mM triethanolamine pH 7.5
Resolution 2.10 Å R-free 0.250
5CJ1 Crystal structure of the coiled coil of MYH7 residues 1526 to 1571 fused to Gp7 Deposited 2015-07-13 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 1526–1571（46 aa） Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571
Chain D 1526–1571（46 aa） Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；16% (w/v) PEG 8000, 400 mM malonate pH 7.2, and 100 mM triethanolamine pH 7.5
Resolution 2.10 Å R-free 0.250
5CJ1 Crystal structure of the coiled coil of MYH7 residues 1526 to 1571 fused to Gp7 Deposited 2015-07-13 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 3 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain E 1526–1571（46 aa） Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571
Chain F 1526–1571（46 aa） Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；16% (w/v) PEG 8000, 400 mM malonate pH 7.2, and 100 mM triethanolamine pH 7.5
Resolution 2.10 Å R-free 0.250
5CJ1 Crystal structure of the coiled coil of MYH7 residues 1526 to 1571 fused to Gp7 Deposited 2015-07-13 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 4 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain G 1526–1571（46 aa） Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571
Chain H 1526–1571（46 aa） Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；16% (w/v) PEG 8000, 400 mM malonate pH 7.2, and 100 mM triethanolamine pH 7.5
Resolution 2.10 Å R-free 0.250
5CJ4 Crystal Structure of Amino Acids 1562-1622 of MYH7 Deposited 2015-07-13 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1562–1622（61 aa） Fragment：UNP Q13426 residues 2-144, UNP P12883 1562-1622
Chain B 1562–1622（61 aa） Fragment：UNP Q13426 residues 2-144, UNP P12883 1562-1622
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；23% (w/v) PEG 4000, 500 mM NaCl, 100 mM triethanolamine pH 8.0.
Resolution 3.10 Å R-free 0.280
5CJ4 Crystal Structure of Amino Acids 1562-1622 of MYH7 Deposited 2015-07-13 Different construct Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 1562–1622（61 aa） Fragment：UNP Q13426 residues 2-144, UNP P12883 1562-1622
Chain D 1562–1622（61 aa） Fragment：UNP Q13426 residues 2-144, UNP P12883 1562-1622
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；23% (w/v) PEG 4000, 500 mM NaCl, 100 mM triethanolamine pH 8.0.
Resolution 3.10 Å R-free 0.280
5TBY HUMAN BETA CARDIAC HEAVY MEROMYOSIN INTERACTING-HEADS MOTIF OBTAINED BY HOMOLOGY MODELING (USING SWISS-MODEL) OF HUMAN SEQUENCE FROM APHONOPELMA HOMOLOGY MODEL (PDB-3JBH), RIGIDLY FITTED TO HUMAN BETA-CARDIAC NEGATIVELY STAINED THICK FILAMENT 3D-RECONSTRUCTION (EMD-2240) Deposited 2016-09-13 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–1935（1935 aa） Fragment：SUBFRAGMENT 1(S1)
Chain B 1–1935（1935 aa） Fragment：SUBFRAGMENT 1(S1)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE；PLUNGING IN A LIQUID ETHANE COOLED BY LIQUID NITROGEN. BLOTTING WAS PERFORMED FROM ONE SIDE OF THE GRID TILL A THIN SAMPLE FILM ON IT USING WHATMAN NO. 42 FILTER PAPER, THEN THE GRID WAS IMMEDIATELY PLUNGED UNDER GRAVITY INTO LIQUID ETHANE COOLED BY LIQUID NITROGEN. GRIDS WERE STORED UNDER LIQUID NITROGEN.
Resolution 20.00 Å
5WJ7 Crystal Structure of Amino Acids 1733-1797 of Human Beta Cardiac Myosin Fused to Xrcc4 Deposited 2017-07-21 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1733–1797（65 aa） Fragment：UNP Q13426 residues 2-132, UNP P12883 residues 1733-1797
Chain B 1733–1797（65 aa） Fragment：UNP Q13426 residues 2-132, UNP P12883 residues 1733-1797
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；16% (w/v) Methyl-Ether PEG 5K, 300 mM glycine, 100 mM triethanolamine pH 7.5
Resolution 2.50 Å R-free 0.249
5WJB Crystal Structure of Amino Acids 1733-1797 of Human Beta Cardiac Myosin Fused to Gp7 Deposited 2017-07-21 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1733–1797（65 aa） Fragment：UNP P13848 residues 2-47, UNP P12833 residues 1733-1797
Chain B 1733–1797（65 aa） Fragment：UNP P13848 residues 2-47, UNP P12833 residues 1733-1797
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；14% (w/v) methyl-ether PEG 2K, 1.5%(w/v) myo-inositol, 100 mM HEPES pH 7.5, 50 mM magnesium chloride
Resolution 2.90 Å R-free 0.300
5WJB Crystal Structure of Amino Acids 1733-1797 of Human Beta Cardiac Myosin Fused to Gp7 Deposited 2017-07-21 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 1733–1797（65 aa） Fragment：UNP P13848 residues 2-47, UNP P12833 residues 1733-1797
Chain D 1733–1797（65 aa） Fragment：UNP P13848 residues 2-47, UNP P12833 residues 1733-1797
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；14% (w/v) methyl-ether PEG 2K, 1.5%(w/v) myo-inositol, 100 mM HEPES pH 7.5, 50 mM magnesium chloride
Resolution 2.90 Å R-free 0.300
5WLQ Crystal Structure of Amino Acids 1677-1755 of Human Beta Cardiac Myosin Fused to Gp7 and Eb1 Deposited 2017-07-27 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1677–1755（79 aa） Fragment：UNP P13848 residues 2-48 UNP Q15691 residues 208-256, UNP P12883 residues 1677-1755
Not recorded SO4 SULFATE ION × 2 TMO trimethylamine oxide × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；298 K；1.6 M Ammonium Aulfate, 500 mM Trimethyl Ammonium N-Oxide, 100 mM Bis-tris Propane pH 9.0
Resolution 3.10 Å R-free 0.236
5WLZ Crystal Structure of Amino Acids 1677-1758 of Human Beta Cardiac Myosin Fused to Xrcc4 Deposited 2017-07-28 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1677–1758（82 aa） Fragment：UNP Q13426 residues 2-132, UNP P12883 residues 1677-1758
Chain B 1677–1758（82 aa） Fragment：UNP Q13426 residues 2-132, UNP P12883 residues 1677-1758
Chain C 1677–1758（82 aa） Fragment：UNP Q13426 residues 2-132, UNP P12883 residues 1677-1758
Chain D 1677–1758（82 aa） Fragment：UNP Q13426 residues 2-132, UNP P12883 residues 1677-1758
Mutation：E29K, E51K, D57A, D58T, E62N, C93R, E98K,C128D, C132A Mutation：E29K, E51K, D57A, D58T, E62N, C93R, E98K,C128D, C132A Mutation：E29K, E51K, D57A, D58T, E62N, C93R, E98K,C128D, C132A Mutation：E29K, E51K, D57A, D58T, E62N, C93R, E98K,C128D, C132A No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；8-10% methyl-ether polyethylene glycol (MEPEG) 5K, 300 mM glycine, bis-tris propane pH 7.0, 1.5-3.0% (w/v) jeffamine M-600
Resolution 3.50 Å R-free 0.249
5WME Crystal Structure of Amino Acids 1729-1786 of Human Beta Cardiac Myosin Fused to Gp7 as Anti-Parallel Four-Helix Bundle Deposited 2017-07-28 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1728–1786（59 aa） Fragment：UNP P13848 residues 2-48, UNP P12883 residues 1729-1786
Chain B 1728–1786（59 aa） Fragment：UNP P13848 residues 2-48, UNP P12883 residues 1729-1786
Chain C 1728–1786（59 aa） Fragment：UNP P13848 residues 2-48, UNP P12883 residues 1729-1786
Chain D 1728–1786（59 aa） Fragment：UNP P13848 residues 2-48, UNP P12883 residues 1729-1786
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5；298 K；18% (w/v) pentaerythritol ethoxylate 797, 150 mM ammonium thiocyanate, 100 mM sodium acetate pH 5.0.
Resolution 2.30 Å R-free 0.264
8ACT structure of the human beta-cardiac myosin folded-back off state Deposited 2022-07-06 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 3–906（904 aa）
Chain B 3–906（904 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) ADP ADENOSINE-5'-DIPHOSPHATE × 2 PO4 PHOSPHATE ION × 2 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8EFD Human cardiac myosin II and associated essential light chain in the rigor conformation Deposited 2022-09-08 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–842（842 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8EFE Human beta-cardiac myosin II bound to ADP-MG2+ and the associated essential light chain Deposited 2022-09-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–842（842 aa）
Not recorded MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8EFH Helical reconstruction of the human cardiac actin-tropomyosin-myosin complex in complex with ADP-Mg2+ Deposited 2022-09-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–842（842 aa）
Not recorded MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8EFI Helical reconstruction of the human cardiac actin-tropomyosin-myosin complex in the rigor form Deposited 2022-09-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain M 1–1935（1935 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8ENC Helical reconstruction of the human cardiac actin-tropomyosin-myosin loop 4 7G mutant complex Deposited 2022-09-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain M 1–1935（1935 aa）
Mutation：Residues 366-372 substituted with seven glycines ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8G4L Cryo-EM structure of the human cardiac myosin filament Deposited 2023-02-10 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 123 PDB declaration: 123-meric
Chain A 1–1935（1935 aa）
Chain AA 1–1935（1935 aa）
Chain AB 1–1935（1935 aa）
Chain AG 1–1935（1935 aa）
Chain AH 1–1935（1935 aa）
Chain AI 1–1935（1935 aa）
Chain AJ 1–1935（1935 aa）
Chain AK 1–1935（1935 aa）
Chain AL 1–1935（1935 aa）
Chain AM 1–1935（1935 aa）
Chain AN 1–1935（1935 aa）
Chain AO 1–1935（1935 aa）
Chain AP 1–1935（1935 aa）
Chain AQ 1–1935（1935 aa）
Chain AR 1–1935（1935 aa）
Chain AS 1–1935（1935 aa）
Chain AT 1–1935（1935 aa）
Chain AU 1–1935（1935 aa）
Chain AV 1–1935（1935 aa）
Chain AW 1–1935（1935 aa）
Chain AX 1–1935（1935 aa）
Chain AY 1–1935（1935 aa）
Chain AZ 1–1935（1935 aa）
Chain B 1–1935（1935 aa）
Chain BA 1–1935（1935 aa）
Chain BB 1–1935（1935 aa）
Chain BG 1–1935（1935 aa）
Chain BH 1–1935（1935 aa）
Chain BI 1–1935（1935 aa）
Chain BJ 1–1935（1935 aa）
Chain BK 1–1935（1935 aa）
Chain BL 1–1935（1935 aa）
Chain BM 1–1935（1935 aa）
Chain BN 1–1935（1935 aa）
Chain BO 1–1935（1935 aa）
Chain BP 1–1935（1935 aa）
Chain BQ 1–1935（1935 aa）
Chain BR 1–1935（1935 aa）
Chain BS 1–1935（1935 aa）
Chain BT 1–1935（1935 aa）
Chain BU 1–1935（1935 aa）
Chain BV 1–1935（1935 aa）
Chain BW 1–1935（1935 aa）
Chain BX 1–1935（1935 aa）
Chain BY 1–1935（1935 aa）
Chain BZ 1–1935（1935 aa）
Chain G 1–1935（1935 aa）
Chain H 1–1935（1935 aa）
Chain I 1–1935（1935 aa）
Chain J 1–1935（1935 aa）
Chain K 1–1935（1935 aa）
Chain L 1–1935（1935 aa）
Chain M 1–1935（1935 aa）
Chain N 1–1935（1935 aa）
Chain O 1–1935（1935 aa）
Chain P 1–1935（1935 aa）
Chain Q 1–1935（1935 aa）
Chain R 1–1935（1935 aa）
Chain S 1–1935（1935 aa）
Chain T 1–1935（1935 aa）
Chain U 1–1935（1935 aa）
Chain V 1–1935（1935 aa）
Chain W 1–1935（1935 aa）
Chain X 1–1935（1935 aa）
Chain Y 1–1935（1935 aa）
Chain Z 1–1935（1935 aa）
Chain ae 1–1935（1935 aa）
Chain af 1–1935（1935 aa）
Chain ak 1–1935（1935 aa）
Chain al 1–1935（1935 aa）
Chain be 1–1935（1935 aa）
Chain bf 1–1935（1935 aa）
Chain bk 1–1935（1935 aa）
Chain bl 1–1935（1935 aa）
Chain e 1–1935（1935 aa）
Chain f 1–1935（1935 aa）
Chain k 1–1935（1935 aa）
Chain l 1–1935（1935 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.40 Å
8ZB7 Human left ventricle ATM complex Deposited 2024-04-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain G 6–781（776 aa）
Chain H 6–781（776 aa）
Chain I 6–781（776 aa）
Chain J 6–781（776 aa）
Chain K 6–781（776 aa）
Chain M 6–781（776 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.19 Å
8ZI9 Human left ventricle actin and myosin complex Deposited 2024-05-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain M 6–781（776 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.08 Å
9GZ1 Beta-cardiac myosin interacting heads motif complexed to mavacamten Deposited 2024-10-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 2–1138（1137 aa）
Chain B 2–1138（1137 aa）
Not recorded MG MAGNESIUM ION × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 2 PO4 PHOSPHATE ION × 2 XB2 Mavacamten × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.2
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
9GZ2 Beta-cardiac heavy meromyosin motor domain in the primed state complexed to mavacamten Deposited 2024-10-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 2–1138（1137 aa）
Not recorded MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1 XB2 Mavacamten × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.2
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9GZ3 Beta-cardiac heavy meromyosin motor domain in the primed state Deposited 2024-10-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 2–1138（1137 aa）
Not recorded MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.2
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
9HTF Beta-cardiac myosin Y115H mutant motor domain in the pre-powerstroke state, MgADP.VO4 form Deposited 2024-12-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–810（810 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 VO4 VANADATE ION × 1 GOL GLYCEROL × 1 EDO 1,2-ETHANEDIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；23 % PEG 3350 w:v, 0.3 M lithium sulfate, 0.1M Tris-HCl, 2 mM Mg.ADP.Vanadate
Resolution 2.48 Å R-free 0.255
9HTG Beta-cardiac myosin E497D mutant motor domain in the pre-powerstroke state, MgADP.VO4 form Deposited 2024-12-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–808（808 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 VO4 VANADATE ION × 1 GOL GLYCEROL × 1 EDO 1,2-ETHANEDIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；22 % PEG 3350 w:v, 0.3 M lithium sulfate, 0.1M Tris-HCl, 2 mM Mg.ADP.Vanadate
Resolution 2.60 Å R-free 0.248
9I8P Human beta-cardiac myosin wild type motor domain in the pre-powerstroke state, MgADP.VO4 form Deposited 2025-02-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–808（808 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 VO4 VANADATE ION × 1 EDO 1,2-ETHANEDIOL × 2 SO4 SULFATE ION × 2 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；25 % PEG 3350 w:v, 0.25 M lithium sulfate, 0.1M Tris-HCl, 2 mM Mg.ADP.Vanadate. Optimal crystals were obtained using the micro-seeding technique.
Resolution 2.60 Å R-free 0.245
9YOP Cryo-EM structure of human beta-cardiac myosin in the interacting-heads motif and S2-FH docked state Deposited 2025-10-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–1016（1016 aa）
Chain B 1–1016（1016 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 2 PO4 PHOSPHATE ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
9YP4 Cryo-EM structure of human beta-cardiac myosin bound to omecamtiv mecarbil in the interacting-heads motif and S2-FH docked state Deposited 2025-10-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–1016（1016 aa）
Chain B 1–1016（1016 aa）
Not recorded 2OW methyl 4-(2-fluoro-3-{[(6-methylpyridin-3-yl)carbamoyl]amino}benzyl)piperazine-1-carboxylate × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 PO4 PHOSPHATE ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.50 Å
9YP9 Cryo-EM structure of human beta-cardiac myosin bound to mavacamten in the interacting-heads motif and S2-FH docked state Deposited 2025-10-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–1016（1016 aa）
Chain B 1–1016（1016 aa）
Not recorded PO4 PHOSPHATE ION × 2 XB2 Mavacamten × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YR7 Cryo-EM structure of human beta-cardiac myosin bound to mavacamten in the interacting-heads motif and S2-FH undocked state Deposited 2025-10-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–1016（1016 aa）
Chain B 1–1016（1016 aa）
Not recorded XB2 Mavacamten × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 PO4 PHOSPHATE ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YRG Cryo-EM structure of human beta-cardiac myosin in the interacting-heads motif and S2-FH undocked state Deposited 2025-10-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–1016（1016 aa）
Chain B 1–1016（1016 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 2 PO4 PHOSPHATE ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
9YRH Cryo-EM structure of human beta-cardiac myosin bound to omecamtiv mecarbil in the interacting-heads motif and S2-FH undocked state Deposited 2025-10-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–1016（1016 aa）
Chain B 1–1016（1016 aa）
Not recorded 2OW methyl 4-(2-fluoro-3-{[(6-methylpyridin-3-yl)carbamoyl]amino}benzyl)piperazine-1-carboxylate × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 PO4 PHOSPHATE ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å

40 other PDB entries and 54 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name MYH7_HUMAN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 54–132； UniProt 1777–1855 Author chain B； PDBConstruct 54–132； UniProt 1777–1855 Author chain C； PDBConstruct 54–132； UniProt 1777–1855 Author chain D； PDBConstruct 54–132； UniProt 1777–1855

Gp7-MYH7(1777-1855)-EB1 chimera protein

Homo sapiens

UniProt P13848

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Insufficient information Homooligomer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count Chain A； UniProt 2–50 Chain B； UniProt 2–50 Fragment：UNP P13848 residues 2-50,UNP P02564 residues 1777-1855,UNP Q15691 residues 209-251 Non-standard monomer：Yes (specific site not provided by mmCIF) No other associated polymer X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 6.4；298 K；18% (w/v) polyethylene glycol 2000 methyl ether, 100 mM piperazine-N,N-bis(2-ethanesulfonic acid) (PIPES) Resolution 3.42 Å R-free 0.297
2 Insufficient information Homooligomer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count Chain C； UniProt 2–50 Chain D； UniProt 2–50 Fragment：UNP P13848 residues 2-50,UNP P02564 residues 1777-1855,UNP Q15691 residues 209-251 Non-standard monomer：Yes (specific site not provided by mmCIF) No other associated polymer X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 6.4；298 K；18% (w/v) polyethylene glycol 2000 methyl ether, 100 mM piperazine-N,N-bis(2-ethanesulfonic acid) (PIPES) Resolution 3.42 Å R-free 0.297

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4XA6 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1NO4 Crystal Structure of the pre-assembly scaffolding protein gp7 from the double-stranded DNA bacteriophage phi29 Deposited 2003-01-15 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–97（97 aa）
Chain B 1–97（97 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；4 % PEG 8000, 0.16 M calcium acetate, 0.08 M sodium cacodylate, 20 % glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.20 Å R-free 0.262
1NO4 Crystal Structure of the pre-assembly scaffolding protein gp7 from the double-stranded DNA bacteriophage phi29 Deposited 2003-01-15 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 1–97（97 aa）
Chain D 1–97（97 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；4 % PEG 8000, 0.16 M calcium acetate, 0.08 M sodium cacodylate, 20 % glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.20 Å R-free 0.262
1NO4 Crystal Structure of the pre-assembly scaffolding protein gp7 from the double-stranded DNA bacteriophage phi29 Deposited 2003-01-15 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–97（97 aa）
Chain B 1–97（97 aa）
Chain C 1–97（97 aa）
Chain D 1–97（97 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；4 % PEG 8000, 0.16 M calcium acetate, 0.08 M sodium cacodylate, 20 % glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.20 Å R-free 0.262
1NOH The structure of bacteriophage phi29 scaffolding protein gp7 after prohead assembly Deposited 2003-01-16 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–97（97 aa）
Chain B 1–97（97 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；14.4 % Peg 8000, 0.08 M sodium cacodylate, 0.16 M calcium acetate, 20 % glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.80 Å R-free 0.290
1NOH The structure of bacteriophage phi29 scaffolding protein gp7 after prohead assembly Deposited 2003-01-16 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 1–97（97 aa）
Chain D 1–97（97 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；14.4 % Peg 8000, 0.08 M sodium cacodylate, 0.16 M calcium acetate, 20 % glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.80 Å R-free 0.290
1NOH The structure of bacteriophage phi29 scaffolding protein gp7 after prohead assembly Deposited 2003-01-16 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–97（97 aa）
Chain B 1–97（97 aa）
Chain C 1–97（97 aa）
Chain D 1–97（97 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；14.4 % Peg 8000, 0.08 M sodium cacodylate, 0.16 M calcium acetate, 20 % glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.80 Å R-free 0.290
3MTU Structure of the Tropomyosin Overlap Complex from Chicken Smooth Muscle Deposited 2010-04-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain E 2–46（45 aa） Fragment：;Fusion protein of residues 2-45 of phage phi29 Gp7 protein and residues 256-284 of chicken smooth muscle tropomyosin,Fusion protein of residues 2-45 of phage phi29 Gp7 protein and residues 256-284 of chicken smooth muscle tropomyosin ;
Chain F 2–46（45 aa） Fragment：;Fusion protein of residues 2-45 of phage phi29 Gp7 protein and residues 256-284 of chicken smooth muscle tropomyosin,Fusion protein of residues 2-45 of phage phi29 Gp7 protein and residues 256-284 of chicken smooth muscle tropomyosin ;
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) CL CHLORIDE ION × 2 EDO 1,2-ETHANEDIOL × 9 EOH ETHANOL × 7 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；16% MePEG 5000, 100 mM MES, pH 6.0, 140 mM CaCl2, 2% methanol , VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.10 Å R-free 0.241
3MTU Structure of the Tropomyosin Overlap Complex from Chicken Smooth Muscle Deposited 2010-04-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Heteromer；Protein × 5 PDB declaration: pentameric
Chain F 2–46（45 aa） Fragment：;Fusion protein of residues 2-45 of phage phi29 Gp7 protein and residues 256-284 of chicken smooth muscle tropomyosin,Fusion protein of residues 2-45 of phage phi29 Gp7 protein and residues 256-284 of chicken smooth muscle tropomyosin ;
Non-standard monomer：Yes (specific site not provided by mmCIF) CL CHLORIDE ION × 2 EDO 1,2-ETHANEDIOL × 9 EOH ETHANOL × 7 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；16% MePEG 5000, 100 mM MES, pH 6.0, 140 mM CaCl2, 2% methanol , VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.10 Å R-free 0.241
3OA7 Structure of the C-terminal domain of Cnm67, a core component of the spindle pole body of Saccharomyces cerevisiae Deposited 2010-08-04 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 2–50（49 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；293 K；10% monomethyl PEG 5000, 1.0M tetramethyl ammonium chloride, 100 mM CHES, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.30 Å R-free 0.271
4IFF Structural organization of FtsB, a transmembrane protein of the bacterial divisome Deposited 2012-12-14 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 2–48（47 aa） Fragment：UNP P13848 residues 2-48, UNP P0A6S5 residues 28-63
Chain B 2–48（47 aa） Fragment：UNP P13848 residues 2-48, UNP P0A6S5 residues 28-63
Not recorded GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；290 K；2.1 M ammonium sulfate, 0.6 M malonate, 5% glycerol, 100 mM Bis-Tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 290 K
Resolution 2.30 Å R-free 0.256
4IFF Structural organization of FtsB, a transmembrane protein of the bacterial divisome Deposited 2012-12-14 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 2–48（47 aa） Fragment：UNP P13848 residues 2-48, UNP P0A6S5 residues 28-63
Chain D 2–48（47 aa） Fragment：UNP P13848 residues 2-48, UNP P0A6S5 residues 28-63
Not recorded GOL GLYCEROL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；290 K；2.1 M ammonium sulfate, 0.6 M malonate, 5% glycerol, 100 mM Bis-Tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 290 K
Resolution 2.30 Å R-free 0.256
4XA1 Crystal Structure of the coiled-coil surrounding Skip 1 of MYH7 Deposited 2014-12-12 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–49（49 aa） Fragment：UNP P13848 residues 1-49,UNP Q12883 residues 1173-1238,UNP Q15691 residues 211-251
Chain B 1–49（49 aa） Fragment：UNP P13848 residues 1-49,UNP Q12883 residues 1173-1238,UNP Q15691 residues 211-251
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.6；298 K；20% (w/v) polyethylene glycol methyl ether 2000, 20 mM SrCl2, 100 mM HEPES pH 7.6, 5% pentaerythritol ethoxylate (17/8 PO/OH) 797, 0.5% 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS)
Resolution 3.20 Å R-free 0.285
4XA1 Crystal Structure of the coiled-coil surrounding Skip 1 of MYH7 Deposited 2014-12-12 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 1–49（49 aa） Fragment：UNP P13848 residues 1-49,UNP Q12883 residues 1173-1238,UNP Q15691 residues 211-251
Chain D 1–49（49 aa） Fragment：UNP P13848 residues 1-49,UNP Q12883 residues 1173-1238,UNP Q15691 residues 211-251
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.6；298 K；20% (w/v) polyethylene glycol methyl ether 2000, 20 mM SrCl2, 100 mM HEPES pH 7.6, 5% pentaerythritol ethoxylate (17/8 PO/OH) 797, 0.5% 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS)
Resolution 3.20 Å R-free 0.285
4XA3 Crystal structure of the coiled-coil surrounding Skip 2 of MYH7 Deposited 2014-12-12 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–49（49 aa） Fragment：UNP P13848 residues 1-49,UNP P12883 residues 1361-1425,UNP Q15691 residues 215-251
Chain B 1–49（49 aa） Fragment：UNP P13848 residues 1-49,UNP P12883 residues 1361-1425,UNP Q15691 residues 215-251
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5；298 K；4.5% (w/v) polyethylene glycol 8000, 100 mM sodium acetate pH 5.0, 50 mM CaCl2, 2.5% (w/v) 3-methoxy-3-methyl-1-butanol
Resolution 2.55 Å R-free 0.311
5CJ1 Crystal structure of the coiled coil of MYH7 residues 1526 to 1571 fused to Gp7 Deposited 2015-07-13 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 2–52（51 aa） Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571
Chain B 2–52（51 aa） Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；16% (w/v) PEG 8000, 400 mM malonate pH 7.2, and 100 mM triethanolamine pH 7.5
Resolution 2.10 Å R-free 0.250
5CJ1 Crystal structure of the coiled coil of MYH7 residues 1526 to 1571 fused to Gp7 Deposited 2015-07-13 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 2–52（51 aa） Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571
Chain D 2–52（51 aa） Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；16% (w/v) PEG 8000, 400 mM malonate pH 7.2, and 100 mM triethanolamine pH 7.5
Resolution 2.10 Å R-free 0.250
5CJ1 Crystal structure of the coiled coil of MYH7 residues 1526 to 1571 fused to Gp7 Deposited 2015-07-13 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 3 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain E 2–52（51 aa） Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571
Chain F 2–52（51 aa） Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；16% (w/v) PEG 8000, 400 mM malonate pH 7.2, and 100 mM triethanolamine pH 7.5
Resolution 2.10 Å R-free 0.250
5CJ1 Crystal structure of the coiled coil of MYH7 residues 1526 to 1571 fused to Gp7 Deposited 2015-07-13 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 4 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain G 2–52（51 aa） Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571
Chain H 2–52（51 aa） Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；16% (w/v) PEG 8000, 400 mM malonate pH 7.2, and 100 mM triethanolamine pH 7.5
Resolution 2.10 Å R-free 0.250
5WJB Crystal Structure of Amino Acids 1733-1797 of Human Beta Cardiac Myosin Fused to Gp7 Deposited 2017-07-21 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 2–48（47 aa） Fragment：UNP P13848 residues 2-47, UNP P12833 residues 1733-1797
Chain B 2–48（47 aa） Fragment：UNP P13848 residues 2-47, UNP P12833 residues 1733-1797
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；14% (w/v) methyl-ether PEG 2K, 1.5%(w/v) myo-inositol, 100 mM HEPES pH 7.5, 50 mM magnesium chloride
Resolution 2.90 Å R-free 0.300
5WJB Crystal Structure of Amino Acids 1733-1797 of Human Beta Cardiac Myosin Fused to Gp7 Deposited 2017-07-21 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 2–48（47 aa） Fragment：UNP P13848 residues 2-47, UNP P12833 residues 1733-1797
Chain D 2–48（47 aa） Fragment：UNP P13848 residues 2-47, UNP P12833 residues 1733-1797
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；14% (w/v) methyl-ether PEG 2K, 1.5%(w/v) myo-inositol, 100 mM HEPES pH 7.5, 50 mM magnesium chloride
Resolution 2.90 Å R-free 0.300
5WLQ Crystal Structure of Amino Acids 1677-1755 of Human Beta Cardiac Myosin Fused to Gp7 and Eb1 Deposited 2017-07-27 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 2–48（47 aa） Fragment：UNP P13848 residues 2-48 UNP Q15691 residues 208-256, UNP P12883 residues 1677-1755
Not recorded SO4 SULFATE ION × 2 TMO trimethylamine oxide × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；298 K；1.6 M Ammonium Aulfate, 500 mM Trimethyl Ammonium N-Oxide, 100 mM Bis-tris Propane pH 9.0
Resolution 3.10 Å R-free 0.236
5WME Crystal Structure of Amino Acids 1729-1786 of Human Beta Cardiac Myosin Fused to Gp7 as Anti-Parallel Four-Helix Bundle Deposited 2017-07-28 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 2–47（46 aa） Fragment：UNP P13848 residues 2-48, UNP P12883 residues 1729-1786
Chain B 2–47（46 aa） Fragment：UNP P13848 residues 2-48, UNP P12883 residues 1729-1786
Chain C 2–47（46 aa） Fragment：UNP P13848 residues 2-48, UNP P12883 residues 1729-1786
Chain D 2–47（46 aa） Fragment：UNP P13848 residues 2-48, UNP P12883 residues 1729-1786
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5；298 K；18% (w/v) pentaerythritol ethoxylate 797, 150 mM ammonium thiocyanate, 100 mM sodium acetate pH 5.0.
Resolution 2.30 Å R-free 0.264
6YJD Lamin A coil2 dimer stabilized by N-terminal capping Deposited 2020-04-03 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–48（48 aa）
Mutation：F40C NI NICKEL (II) ION × 4 CL CHLORIDE ION × 2 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.2；277 K；35% (v/v) methanol, 0.2 M MgCl2 and 0.1 M HEPES
Resolution 2.90 Å R-free 0.304
8F2M Phi-29 scaffolding protein bound to intermediate-state MCP Deposited 2022-11-08 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain B 1–98（98 aa）
Chain C 1–98（98 aa）
Chain F 1–98（98 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å

13 other PDB entries and 24 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name VG7_BPPH2
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 5–53； UniProt 2–50 Author chain B； PDBConstruct 5–53； UniProt 2–50 Author chain C； PDBConstruct 5–53； UniProt 2–50 Author chain D； PDBConstruct 5–53； UniProt 2–50

Gp7-MYH7(1777-1855)-EB1 chimera protein

Homo sapiens

UniProt Q15691

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Insufficient information Homooligomer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count Chain A； UniProt 209–251 Chain B； UniProt 209–251 Fragment：UNP P13848 residues 2-50,UNP P02564 residues 1777-1855,UNP Q15691 residues 209-251 Non-standard monomer：Yes (specific site not provided by mmCIF) No other associated polymer X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 6.4；298 K；18% (w/v) polyethylene glycol 2000 methyl ether, 100 mM piperazine-N,N-bis(2-ethanesulfonic acid) (PIPES) Resolution 3.42 Å R-free 0.297
2 Insufficient information Homooligomer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count Chain C； UniProt 209–251 Chain D； UniProt 209–251 Fragment：UNP P13848 residues 2-50,UNP P02564 residues 1777-1855,UNP Q15691 residues 209-251 Non-standard monomer：Yes (specific site not provided by mmCIF) No other associated polymer X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 6.4；298 K；18% (w/v) polyethylene glycol 2000 methyl ether, 100 mM piperazine-N,N-bis(2-ethanesulfonic acid) (PIPES) Resolution 3.42 Å R-free 0.297

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4XA6 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1PA7 Crystal structure of amino-terminal microtubule binding domain of EB1 Deposited 2003-05-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–130（130 aa） Fragment：N-terminal domain, EB1 microtubule-binding domain
Not recorded SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；298 K；PEG4K, ammonium sulfate, MES, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 1.45 Å R-free 0.189
1TXQ Crystal structure of the EB1 C-terminal domain complexed with the CAP-Gly domain of p150Glued Deposited 2004-07-06 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain B 183–268（86 aa） Fragment：EB1 C-terminal domain
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；ammonium sulphate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 1.80 Å R-free 0.243
1UEG Crystal structure of amino-terminal microtubule binding domain of EB1 Deposited 2003-05-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–130（130 aa） Fragment：N-terminal domain, EB1 microtubule-binding domain
Not recorded SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.5；298 K；ammonium sulfate, sodium citrate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 2.40 Å R-free 0.279
1VKA Southeast Collaboratory for Structural Genomics: Hypothetical Human Protein Q15691 N-Terminal Fragment Deposited 2004-05-10 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 2–140（139 aa） Fragment：N-terminal domain
Chain B 2–140（139 aa） Fragment：N-terminal domain
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions Micro-batch under oil；pH 6.2；291 K；pH 6.2, Micro-batch under oil, temperature 291K
Resolution 1.60 Å R-free 0.214
1WU9 Crystal structure of the C-terminal domain of the end-binding protein 1 (EB1) Deposited 2004-12-02 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 190–267（78 aa） Fragment：C-terminal domain
Chain B 190–267（78 aa） Fragment：C-terminal domain
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.5；298 K；sodium citrate, PEG 3350, gamma-butyrolactone, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 1.54 Å R-free 0.220
1YIB Crystal Structure of the Human EB1 C-terminal Dimerization Domain Deposited 2005-01-11 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 184–254（71 aa） Fragment：EB1 C-terminal Domain
Mutation：N-terminal cloning artifact GPLGS No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.6；293 K；protein stock = EB1 C-terminal domain @ 15 mg/ml, MACF2 (1595-1637) peptide @ 8 mg/ml, well = 22% PEG 200 (v/v), 100 mM ammonium acetate pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.80 Å R-free 0.259
1YIG Crystal Structure of the Human EB1 C-terminal Dimerization Domain Deposited 2005-01-11 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 184–254（71 aa） Fragment：EB1 C-terminal Domain
Chain B 184–254（71 aa） Fragment：EB1 C-terminal Domain
Mutation：N-terminal cloning artifact GPLGS, V243M mutant, methionines at positions 197 and 243 are seleno-methionines Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：N-terminal cloning artifact GPLGS, V243M mutant, methionines at positions 197 and 243 are seleno-methionines Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.6；293 K；protein stock = EB1 C-terminal domain @15 mg/ml, well = 22%, PEG 200 (v/v), 100 mM ammonium acetate pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.00 Å R-free 0.251
2HKQ Crystal structure of the C-terminal domain of human EB1 in complex with the CAP-Gly domain of human Dynactin-1 (p150-Glued) Deposited 2006-07-05 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 188–267（80 aa） Fragment：C-terminal domain
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；1.3 M Trisodium Citrate, 0.1M HEPES Buffer, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 1.86 Å R-free 0.217
2HL3 Crystal structure of the A49M mutant CAP-Gly domain of human Dynactin-1 (p150-Glued) in complex with human EB1 C-terminal hexapeptide Deposited 2006-07-06 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 262–267（6 aa） Fragment：C-terminal hexapeptide
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.5；298 K；25% PEG 3350, 0.05M Sodium Citrate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 2.03 Å R-free 0.223
2HL5 Crystal structure of the C-terminal domain of human EB1 in complex with the A49M mutant CAP-Gly domain of human Dynactin-1 (p150-Glued) Deposited 2006-07-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 190–267（78 aa） Fragment：C-terminal domain
Chain B 190–267（78 aa） Fragment：C-terminal domain
Not recorded CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；25% PEG 3350, 0.1M HEPES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 1.93 Å R-free 0.242
2QJZ Structural Basis of Microtubule Plus End Tracking by XMAP215, CLIP-170 and EB1 Deposited 2007-07-09 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 12–133（122 aa） Fragment：Calponin Homology Domain
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；11.5% 2-propanol, 190 mM Sodium citrate, 100 mM Hepes, Protein concentration 20 mg/ml, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.25 Å R-free 0.198
2QJZ Structural Basis of Microtubule Plus End Tracking by XMAP215, CLIP-170 and EB1 Deposited 2007-07-09 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 12–133（122 aa） Fragment：Calponin Homology Domain
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；11.5% 2-propanol, 190 mM Sodium citrate, 100 mM Hepes, Protein concentration 20 mg/ml, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.25 Å R-free 0.198
2R8U Structure of fragment of human end-binding protein 1 (EB1) containing the N-terminal domain at 1.35 A resolution Deposited 2007-09-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–268（268 aa） Fragment：N-terminal domain
Not recorded MOO MOLYBDATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；293 K；0.1M Tris, 0.1M Na2MoO4, 35% PEG4000, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
Resolution 1.35 Å R-free 0.227
2R8U Structure of fragment of human end-binding protein 1 (EB1) containing the N-terminal domain at 1.35 A resolution Deposited 2007-09-11 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1–268（268 aa） Fragment：N-terminal domain
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；293 K；0.1M Tris, 0.1M Na2MoO4, 35% PEG4000, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
Resolution 1.35 Å R-free 0.227
3GJO Crystal structure of human EB1 in complex with microtubule Tip localization signal peptide of MACF Deposited 2009-03-09 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 191–260（70 aa） Fragment：EB1 C-TERMINAL DOMAIN, UNP RESIDUES 191-260
Chain B 191–260（70 aa） Fragment：EB1 C-TERMINAL DOMAIN, UNP RESIDUES 191-260
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.4；298 K；0.2M MAGNESIUM ACETATE, 20% PEG 3350, pH 7.40, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K
Resolution 2.50 Å R-free 0.255
3GJO Crystal structure of human EB1 in complex with microtubule Tip localization signal peptide of MACF Deposited 2009-03-09 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain C 191–260（70 aa） Fragment：EB1 C-TERMINAL DOMAIN, UNP RESIDUES 191-260
Chain D 191–260（70 aa） Fragment：EB1 C-TERMINAL DOMAIN, UNP RESIDUES 191-260
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.4；298 K；0.2M MAGNESIUM ACETATE, 20% PEG 3350, pH 7.40, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K
Resolution 2.50 Å R-free 0.255
3MTU Structure of the Tropomyosin Overlap Complex from Chicken Smooth Muscle Deposited 2010-04-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 216–257（42 aa） Fragment：;Fusion protein of residues 1-29 of chicken smooth muscle tropomyosin and residues 215-257 of human EB1 protein,Fusion protein of residues 1-29 of chicken smooth muscle tropomyosin and residues 215-257 of human EB1 protein ;
Chain B 216–257（42 aa） Fragment：;Fusion protein of residues 1-29 of chicken smooth muscle tropomyosin and residues 215-257 of human EB1 protein,Fusion protein of residues 1-29 of chicken smooth muscle tropomyosin and residues 215-257 of human EB1 protein ;
Chain C 216–257（42 aa） Fragment：;Fusion protein of residues 1-29 of chicken smooth muscle tropomyosin and residues 215-257 of human EB1 protein,Fusion protein of residues 1-29 of chicken smooth muscle tropomyosin and residues 215-257 of human EB1 protein ;
Chain D 216–257（42 aa） Fragment：;Fusion protein of residues 1-29 of chicken smooth muscle tropomyosin and residues 215-257 of human EB1 protein,Fusion protein of residues 1-29 of chicken smooth muscle tropomyosin and residues 215-257 of human EB1 protein ;
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) CL CHLORIDE ION × 2 EDO 1,2-ETHANEDIOL × 9 EOH ETHANOL × 7 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；16% MePEG 5000, 100 mM MES, pH 6.0, 140 mM CaCl2, 2% methanol , VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.10 Å R-free 0.241
3MTU Structure of the Tropomyosin Overlap Complex from Chicken Smooth Muscle Deposited 2010-04-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Heteromer；Protein × 5 PDB declaration: pentameric
Chain A 216–257（42 aa） Fragment：;Fusion protein of residues 1-29 of chicken smooth muscle tropomyosin and residues 215-257 of human EB1 protein,Fusion protein of residues 1-29 of chicken smooth muscle tropomyosin and residues 215-257 of human EB1 protein ;
Chain B 216–257（42 aa） Fragment：;Fusion protein of residues 1-29 of chicken smooth muscle tropomyosin and residues 215-257 of human EB1 protein,Fusion protein of residues 1-29 of chicken smooth muscle tropomyosin and residues 215-257 of human EB1 protein ;
Chain C 216–257（42 aa） Fragment：;Fusion protein of residues 1-29 of chicken smooth muscle tropomyosin and residues 215-257 of human EB1 protein,Fusion protein of residues 1-29 of chicken smooth muscle tropomyosin and residues 215-257 of human EB1 protein ;
Chain D 216–257（42 aa） Fragment：;Fusion protein of residues 1-29 of chicken smooth muscle tropomyosin and residues 215-257 of human EB1 protein,Fusion protein of residues 1-29 of chicken smooth muscle tropomyosin and residues 215-257 of human EB1 protein ;
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) CL CHLORIDE ION × 2 EDO 1,2-ETHANEDIOL × 9 EOH ETHANOL × 7 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；16% MePEG 5000, 100 mM MES, pH 6.0, 140 mM CaCl2, 2% methanol , VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.10 Å R-free 0.241
3MUD Structure of the Tropomyosin Overlap Complex from Chicken Smooth Muscle Deposited 2010-05-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: tetrameric
Chain C 215–257（43 aa）
Chain D 215–257（43 aa）
Not recorded EDO 1,2-ETHANEDIOL × 1 SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；18% MePEG 2000, 100 mM Bis-Tris, 120 mM MgSO4 , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.20 Å R-free 0.250
3TQ7 EB1c/EB3c heterodimer in complex with the CAP-Gly domain of P150glued Deposited 2011-09-09 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 191–268（78 aa） Fragment：EB1 c-terminal domain
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；15% PEG 4000, 0.1M Tris-HCl, 0.2M Magnesium chloride hexahydrate, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 2.30 Å R-free 0.281
4XA1 Crystal Structure of the coiled-coil surrounding Skip 1 of MYH7 Deposited 2014-12-12 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 211–251（41 aa） Fragment：UNP P13848 residues 1-49,UNP Q12883 residues 1173-1238,UNP Q15691 residues 211-251
Chain B 211–251（41 aa） Fragment：UNP P13848 residues 1-49,UNP Q12883 residues 1173-1238,UNP Q15691 residues 211-251
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.6；298 K；20% (w/v) polyethylene glycol methyl ether 2000, 20 mM SrCl2, 100 mM HEPES pH 7.6, 5% pentaerythritol ethoxylate (17/8 PO/OH) 797, 0.5% 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS)
Resolution 3.20 Å R-free 0.285
4XA1 Crystal Structure of the coiled-coil surrounding Skip 1 of MYH7 Deposited 2014-12-12 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 211–251（41 aa） Fragment：UNP P13848 residues 1-49,UNP Q12883 residues 1173-1238,UNP Q15691 residues 211-251
Chain D 211–251（41 aa） Fragment：UNP P13848 residues 1-49,UNP Q12883 residues 1173-1238,UNP Q15691 residues 211-251
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.6；298 K；20% (w/v) polyethylene glycol methyl ether 2000, 20 mM SrCl2, 100 mM HEPES pH 7.6, 5% pentaerythritol ethoxylate (17/8 PO/OH) 797, 0.5% 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS)
Resolution 3.20 Å R-free 0.285
4XA3 Crystal structure of the coiled-coil surrounding Skip 2 of MYH7 Deposited 2014-12-12 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 215–251（37 aa） Fragment：UNP P13848 residues 1-49,UNP P12883 residues 1361-1425,UNP Q15691 residues 215-251
Chain B 215–251（37 aa） Fragment：UNP P13848 residues 1-49,UNP P12883 residues 1361-1425,UNP Q15691 residues 215-251
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5；298 K；4.5% (w/v) polyethylene glycol 8000, 100 mM sodium acetate pH 5.0, 50 mM CaCl2, 2.5% (w/v) 3-methoxy-3-methyl-1-butanol
Resolution 2.55 Å R-free 0.311
5JV3 The neck-linker and alpha 7 helix of Homo sapiens Eg5 fused to EB1 Deposited 2016-05-10 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 210–257（48 aa） Fragment：UNP P52732 residues 366-391,UNP Q15691 residues 210-257
Chain B 210–257（48 aa） Fragment：UNP P52732 residues 366-391,UNP Q15691 residues 210-257
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；24% (w/v) PEE797, 100 mM CaCl2, 100 mM MOPS pH 7.0, 0.1% (w/v) octylglucoside
Resolution 2.01 Å R-free 0.247
5JV3 The neck-linker and alpha 7 helix of Homo sapiens Eg5 fused to EB1 Deposited 2016-05-10 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 210–257（48 aa） Fragment：UNP P52732 residues 366-391,UNP Q15691 residues 210-257
Chain D 210–257（48 aa） Fragment：UNP P52732 residues 366-391,UNP Q15691 residues 210-257
Not recorded CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；24% (w/v) PEE797, 100 mM CaCl2, 100 mM MOPS pH 7.0, 0.1% (w/v) octylglucoside
Resolution 2.01 Å R-free 0.247
5JVM The neck-linker and alpha 7 helix of Mus musculus KIF3C Deposited 2016-05-11 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 207–257（51 aa） Fragment：UNP O35066 residues 374-402,UNP Q15691 residues 207-257
Chain B 207–257（51 aa） Fragment：UNP O35066 residues 374-402,UNP Q15691 residues 207-257
Not recorded MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；298 K；30% (w/v) pentaerythritol ethoxylate (PEE) 797, 1.5% (w/v) ethylene glycol monoethylether, 400 mM MgCl2, 100 mM bis-tris propane pH 9.0
Resolution 1.57 Å R-free 0.205
5JVP The neck-linker and alpha 7 helix of Homo sapiens CENP-E Deposited 2016-05-11 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 210–257（48 aa） Fragment：UNP Q02224 residues 336-375,UNP Q15691 residues 210-257
Non-standard monomer：Yes (specific site not provided by mmCIF) CD CADMIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；18% (w/v) MEPEG 2000, 175 mM Li2SO4, 100 mM MES pH 6.0, 1 mM CdCl2
Resolution 2.10 Å R-free 0.274
5JVP The neck-linker and alpha 7 helix of Homo sapiens CENP-E Deposited 2016-05-11 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain B 210–257（48 aa） Fragment：UNP Q02224 residues 336-375,UNP Q15691 residues 210-257
Non-standard monomer：Yes (specific site not provided by mmCIF) CD CADMIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；18% (w/v) MEPEG 2000, 175 mM Li2SO4, 100 mM MES pH 6.0, 1 mM CdCl2
Resolution 2.10 Å R-free 0.274
5JVP The neck-linker and alpha 7 helix of Homo sapiens CENP-E Deposited 2016-05-11 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 210–257（48 aa） Fragment：UNP Q02224 residues 336-375,UNP Q15691 residues 210-257
Chain D 210–257（48 aa） Fragment：UNP Q02224 residues 336-375,UNP Q15691 residues 210-257
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) CD CADMIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；18% (w/v) MEPEG 2000, 175 mM Li2SO4, 100 mM MES pH 6.0, 1 mM CdCl2
Resolution 2.10 Å R-free 0.274
5JVP The neck-linker and alpha 7 helix of Homo sapiens CENP-E Deposited 2016-05-11 Different construct Different experimental conditions Different structure-quality metrics Assembly 4 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain E 210–257（48 aa） Fragment：UNP Q02224 residues 336-375,UNP Q15691 residues 210-257
Chain F 210–257（48 aa） Fragment：UNP Q02224 residues 336-375,UNP Q15691 residues 210-257
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；18% (w/v) MEPEG 2000, 175 mM Li2SO4, 100 mM MES pH 6.0, 1 mM CdCl2
Resolution 2.10 Å R-free 0.274
5JVR The neck-linker of Mus musculus KIF3A fused to the alpha 7 helix of Drosophila melanogaster Kinesin-1 fused to EB1 Deposited 2016-05-11 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 207–257（51 aa） Fragment：UNP P28741 residues 352-359,UNP P17210 residues 345-358,UNP Q15691 residues 207-257
Chain B 207–257（51 aa） Fragment：UNP P28741 residues 352-359,UNP P17210 residues 345-358,UNP Q15691 residues 207-257
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；32% (w/v) polyethylene glycol dimethyl ether (DMPEG) 500, 30 mM MgCl2, 10 mM diethylenetriamine, 100 mM sodium 3-[4-(2-Hydroxyethyl)-1-piperazinyl]propanesulfonic acid (HEPPS) pH 8.5
Resolution 2.10 Å R-free 0.234
5JVR The neck-linker of Mus musculus KIF3A fused to the alpha 7 helix of Drosophila melanogaster Kinesin-1 fused to EB1 Deposited 2016-05-11 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 207–257（51 aa） Fragment：UNP P28741 residues 352-359,UNP P17210 residues 345-358,UNP Q15691 residues 207-257
Chain D 207–257（51 aa） Fragment：UNP P28741 residues 352-359,UNP P17210 residues 345-358,UNP Q15691 residues 207-257
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；32% (w/v) polyethylene glycol dimethyl ether (DMPEG) 500, 30 mM MgCl2, 10 mM diethylenetriamine, 100 mM sodium 3-[4-(2-Hydroxyethyl)-1-piperazinyl]propanesulfonic acid (HEPPS) pH 8.5
Resolution 2.10 Å R-free 0.234
5JVR The neck-linker of Mus musculus KIF3A fused to the alpha 7 helix of Drosophila melanogaster Kinesin-1 fused to EB1 Deposited 2016-05-11 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 3 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain E 207–257（51 aa） Fragment：UNP P28741 residues 352-359,UNP P17210 residues 345-358,UNP Q15691 residues 207-257
Chain F 207–257（51 aa） Fragment：UNP P28741 residues 352-359,UNP P17210 residues 345-358,UNP Q15691 residues 207-257
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；32% (w/v) polyethylene glycol dimethyl ether (DMPEG) 500, 30 mM MgCl2, 10 mM diethylenetriamine, 100 mM sodium 3-[4-(2-Hydroxyethyl)-1-piperazinyl]propanesulfonic acid (HEPPS) pH 8.5
Resolution 2.10 Å R-free 0.234
5JVR The neck-linker of Mus musculus KIF3A fused to the alpha 7 helix of Drosophila melanogaster Kinesin-1 fused to EB1 Deposited 2016-05-11 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 4 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain G 207–257（51 aa） Fragment：UNP P28741 residues 352-359,UNP P17210 residues 345-358,UNP Q15691 residues 207-257
Chain H 207–257（51 aa） Fragment：UNP P28741 residues 352-359,UNP P17210 residues 345-358,UNP Q15691 residues 207-257
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；32% (w/v) polyethylene glycol dimethyl ether (DMPEG) 500, 30 mM MgCl2, 10 mM diethylenetriamine, 100 mM sodium 3-[4-(2-Hydroxyethyl)-1-piperazinyl]propanesulfonic acid (HEPPS) pH 8.5
Resolution 2.10 Å R-free 0.234
5JVS The neck-linker + DAL and alpha 7 helix of Drosophila melanogaster Kinesin-1 fused to EB1 Deposited 2016-05-11 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 207–257（51 aa） Fragment：UNP P17210 residues 334-365,UNP Q15691 residues 207-257
Not recorded ZN ZINC ION × 2 EDO 1,2-ETHANEDIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5；293 K；100 mM sodium acetate pH 5.0, 24% (w/v) polyethylene glycol (PEG) 400, 80 mM MgCl2, 3 mM ZnSO4
Resolution 2.25 Å R-free 0.236
5JVU The neck-linker and alpha 7 helix of Drosophila melanogaster kinesin-1 fused to EB1 Deposited 2016-05-11 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 207–257（51 aa） Fragment：UNP P17210 residues 334-365,UNP Q15691 residues 207-257
Chain B 207–257（51 aa） Fragment：UNP P17210 residues 334-365,UNP Q15691 residues 207-257
Not recorded EDO 1,2-ETHANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；20% (w/v) methoxy polyethylene glycol (MEPEG) 5000, 100 mM Li2SO4, 100 mM MES/Acetate pH 5.5, 10 mM gamma-caprolactone
Resolution 1.95 Å R-free 0.243
5JX1 The neck-linker and alpha 7 helix of Mus musculus KIF3A fused to EB1 Deposited 2016-05-12 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 210–257（48 aa） Fragment：UNP P28741 residues 352-376,UNP Q15691 residues 210-257
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；24% (w/v) 2-methyl-2,4-pentanediol (MPD), 10% (w/v) PEG 4000, 100 mM CaCl2, 100 mM (3-(N-morpholino)propanesulfonic acid) (MOPS) pH 7.0, 1 mM CdCl2
Resolution 1.67 Å R-free 0.228
5N74 Microtubule end binding protein complex Deposited 2017-02-18 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 191–248（58 aa）
Chain B 191–248（58 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；293 K；100 mM PCTP buffer, pH 4, supplemented with 25% PEG 1500
Resolution 2.30 Å R-free 0.271
5N74 Microtubule end binding protein complex Deposited 2017-02-18 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain C 191–248（58 aa）
Chain D 191–248（58 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；293 K；100 mM PCTP buffer, pH 4, supplemented with 25% PEG 1500
Resolution 2.30 Å R-free 0.271
5N74 Microtubule end binding protein complex Deposited 2017-02-18 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain E 191–248（58 aa）
Chain F 191–248（58 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；293 K；100 mM PCTP buffer, pH 4, supplemented with 25% PEG 1500
Resolution 2.30 Å R-free 0.271
5N74 Microtubule end binding protein complex Deposited 2017-02-18 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 4 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain G 191–248（58 aa）
Chain H 191–248（58 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；293 K；100 mM PCTP buffer, pH 4, supplemented with 25% PEG 1500
Resolution 2.30 Å R-free 0.271
5WLQ Crystal Structure of Amino Acids 1677-1755 of Human Beta Cardiac Myosin Fused to Gp7 and Eb1 Deposited 2017-07-27 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 208–257（50 aa） Fragment：UNP P13848 residues 2-48 UNP Q15691 residues 208-256, UNP P12883 residues 1677-1755
Not recorded SO4 SULFATE ION × 2 TMO trimethylamine oxide × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；298 K；1.6 M Ammonium Aulfate, 500 mM Trimethyl Ammonium N-Oxide, 100 mM Bis-tris Propane pH 9.0
Resolution 3.10 Å R-free 0.236
6YF5 Lamin A 17-70 coil1A dimer stabilized by C-terminal capping Deposited 2020-03-26 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 215–251（37 aa）
Chain B 215–251（37 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.9；277 K；35.7% (w/v) 1,6-hexanediol, 5% (w/v) PEG 1K and 0.1 M trisodium citrate dihydrate
Resolution 1.83 Å R-free 0.255
6YF5 Lamin A 17-70 coil1A dimer stabilized by C-terminal capping Deposited 2020-03-26 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 215–251（37 aa）
Chain D 215–251（37 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.9；277 K；35.7% (w/v) 1,6-hexanediol, 5% (w/v) PEG 1K and 0.1 M trisodium citrate dihydrate
Resolution 1.83 Å R-free 0.255
6YSH Lamin A 1-70 coil1A dimer stabilized by C-terminal capping Deposited 2020-04-22 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 215–251（37 aa）
Chain B 215–250（36 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；298 K；0.1M NaH2PO4 H2O,0.1M KH2PO4,2.0M NaCl,0.1M Mes H20 pH 6
Resolution 2.83 Å R-free 0.281
9CO5 Crystal Structure of Macrocycle mediated complex of FKBP12 and MAPRE1 Deposited 2024-07-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain B 193–252（60 aa）
Not recorded A1AZI (5S,14R,16aS,21R,28S,30aR)-14-[2-(3,4-dimethoxyphenyl)ethyl]-24,24,28-trimethyl-2-methylidene-1,3,4,17,18,19,20,24,25,28,29,30a-dodecahydro-2H,14H-9,13-(metheno)dipyrido[1,2-d:1',2'-o][1,10,18,4,7,15]trioxatriazacyclotetracosine-6,16,22,23,27,30(7H,16aH)-hexone × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；1.5 M LiSO4, 0.1 M CH3COONa pH 4.6
Resolution 2.77 Å R-free 0.225
9SMX CM1-activated gTuRC in complex with nascent alpha-E254D mutant microtubules Deposited 2025-09-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 73 PDB declaration: 73-meric
Chain AC 190–255（66 aa）
Chain CC 190–255（66 aa）
Chain Cc 190–255（66 aa）
Chain EC 190–255（66 aa）
Chain Ec 190–255（66 aa）
Chain GC 190–255（66 aa）
Chain Gc 190–255（66 aa）
Chain MC 190–255（66 aa）
Chain Mc 190–255（66 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 11 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.67 Å

31 other PDB entries and 47 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name MARE1_HUMAN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 133–175； UniProt 209–251 Author chain B； PDBConstruct 133–175； UniProt 209–251 Author chain C； PDBConstruct 133–175； UniProt 209–251 Author chain D； PDBConstruct 133–175； UniProt 209–251

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 4xa6

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 4xa6
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2. Structure Basics 2. Structure Basics

Entry ID entry_id4xa6
Deposition date deposition_date2014-12-12
Structure title titleCrystal Structure of the coiled-coil surrounding Skip 4 of MYH7
Keywords keywordsMyosin, coiled coil, skip residue, fusion, Gp7, EB1, MYH7, Cardiac, MOTOR PROTEIN; MOTOR PROTEIN
Experimental Method methodX-RAY DIFFRACTION
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier53.01 Å
Radius of gyration Rg (electron density) rg_electron54.70 Å
Forward intensity I(0) i070956300.00
Molecular weight molecular_weight67756.0 kDa
Excluded volume excluded_volume84637 ų
Envelope volume envelope_volume141310 ų
Hydration-shell volume shell_volume26890 ų
Envelope diameter envelope_diameter200.6 Å
Shell Rg shell_rg42.91 Å
Envelope Rg envelope_rg53.80 Å
Shape Rg shape_rg54.75 Å
Total Rg total_rg53.95 Å
Total atoms total_atoms4726
Residues n_residues488
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax207.0 Å
Rg (real space) rg_real54.08 Å
Rg uncertainty (real space) rg_real_error3.99 Å
I(0) (real space) i0_real7.0960e+07
I(0) uncertainty (real space) i0_real_error1.7010e+06
Rg (reciprocal space) rg_reciprocal52.12 Å
I(0) (reciprocal space) i0_reciprocal70760000.0000
Solution quality estimate total_estimate0.6322
Solution quality rating solution_quality REASONABLE a REASONABLE solution
P(r) peaks n_peaks1
Primary peak position r_peak_primary34.5 Å
Skewness Skewness skewness0.653
Kurtosis Kurtosis kurtosis-0.358
Angular range angular_range— – 0.1500 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha3254000.0000
Real-space data points n_real_points31
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.172; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.112; Smooth: 0.588
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (1)

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8. Citations (1)

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9. Files and Curves (10)