SAXSdb — 8qjh

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Gap junction beta-1 protein

Homo sapiens

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein homooligomer Homooligomer Protein × 12 PDB declaration: 12-meric（12） Consistent with protein copy count	Chain A； UniProt 1–283 Chain B； UniProt 1–283 Chain C； UniProt 1–283 Chain D； UniProt 1–283 Chain E； UniProt 1–283 Chain F； UniProt 1–283 Chain G； UniProt 1–283 Chain H； UniProt 1–283 Chain I； UniProt 1–283 Chain J； UniProt 1–283 Chain K； UniProt 1–283 Chain L； UniProt 1–283	Not recorded	No other associated polymer	ELECTRON MICROSCOPY cryo-EM buffer：pH 8 cryo-EM vitrification conditions：Cryogen ETHANE	Resolution 2.91 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 8QJH	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
5KK9 Connexin 32 G12R N-Terminal Mutant, Deposited 2016-06-21	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–22（22 aa）	Mutation：G12R Non-standard monomer：Yes (specific site not provided by mmCIF)	No recorded non-water small molecule	SOLUTION NMR NMR measurement conditions pH 7；288 K；Ionic strength (raw mmCIF value) 0.1；Pressure 1 NMR sample composition 1.0 mM ACE-MET-ASN-TRP-THR-GLY-LEU-TYR-THR-LEU-LEU-SER-ARG-VAL-ASN-ARG-HIS-SER-THR-ALA-ILE-GLY-ARG, 100 mM KCl, 100 uM TSP, 90% H2O/10% D2O \| 90% H2O/10% D2O	Resolution not provided
7ZXM cryo-EM structure of Connexin 32 gap junction channel Deposited 2022-05-21	Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: dodecameric	Chain A 1–283（283 aa） Chain B 1–283（283 aa） Chain C 1–283（283 aa） Chain D 1–283（283 aa） Chain E 1–283（283 aa） Chain F 1–283（283 aa） Chain G 1–283（283 aa） Chain H 1–283（283 aa） Chain I 1–283（283 aa） Chain J 1–283（283 aa） Chain K 1–283（283 aa） Chain L 1–283（283 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.14 Å
7ZXN cryo-EM structure of Connexin 32 gap junction channel Deposited 2022-05-21	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric	Chain A 1–283（283 aa） Chain B 1–283（283 aa） Chain C 1–283（283 aa） Chain D 1–283（283 aa） Chain E 1–283（283 aa） Chain F 1–283（283 aa）	Not recorded	CLR CHOLESTEROL × 6	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.06 Å
7ZXO cryo-EM structure of Connexin 32 gap junction channel Deposited 2022-05-21	Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: dodecameric	Chain A 1–283（283 aa） Chain B 1–283（283 aa） Chain C 1–283（283 aa） Chain D 1–283（283 aa） Chain E 1–283（283 aa） Chain F 1–283（283 aa） Chain G 1–283（283 aa） Chain H 1–283（283 aa） Chain I 1–283（283 aa） Chain J 1–283（283 aa） Chain K 1–283（283 aa） Chain L 1–283（283 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.50 Å
7ZXP cryo-EM structure of Connexin 32 R22G mutation gap junction channel Deposited 2022-05-22	Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: dodecameric	Chain A 1–283（283 aa） Chain B 1–283（283 aa） Chain C 1–283（283 aa） Chain D 1–283（283 aa） Chain E 1–283（283 aa） Chain F 1–283（283 aa） Chain G 1–283（283 aa） Chain H 1–283（283 aa） Chain I 1–283（283 aa） Chain J 1–283（283 aa） Chain K 1–283（283 aa） Chain L 1–283（283 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.39 Å
7ZXQ cryo-EM structure of Connexin 32 R22G mutation hemi channel Deposited 2022-05-22	Different construct Different mutation/modification Different oligomeric state Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric	Chain A 1–283（283 aa） Chain B 1–283（283 aa） Chain C 1–283（283 aa） Chain D 1–283（283 aa） Chain E 1–283（283 aa） Chain F 1–283（283 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.53 Å
7ZXT cryo-EM structure of Connexin 32 W3S mutation hemi channel Deposited 2022-05-22	Different construct Different mutation/modification Different oligomeric state Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric	Chain A 1–283（283 aa） Chain B 1–283（283 aa） Chain C 1–283（283 aa） Chain D 1–283（283 aa） Chain E 1–283（283 aa） Chain F 1–283（283 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
8QJF Connexin-32 gap junction channel in complex with 2-aminoethoxydiphenyl borate Deposited 2023-09-13	Different ligand/ion Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: 12-meric	Chain A 1–283（283 aa） Chain C 1–283（283 aa） Chain D 1–283（283 aa） Chain E 1–283（283 aa） Chain F 1–283（283 aa） Chain G 1–283（283 aa） Chain H 1–283（283 aa） Chain I 1–283（283 aa） Chain J 1–283（283 aa） Chain K 1–283（283 aa） Chain L 1–283（283 aa） Chain M 1–283（283 aa）	Not recorded	CLR CHOLESTEROL × 12	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.86 Å
8QK6 Connexin-32 hemichannel upon addition of 2-aminoetoxydiphenyl borate Deposited 2023-09-14	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric	Chain A 1–283（283 aa） Chain B 1–283（283 aa） Chain C 1–283（283 aa） Chain D 1–283（283 aa） Chain E 1–283（283 aa） Chain F 1–283（283 aa）	Not recorded	CLR CHOLESTEROL × 6	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.18 Å
8QKI Connexin-32 hemichannel upon addition of mefloquine Deposited 2023-09-15	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric	Chain A 1–283（283 aa） Chain B 1–283（283 aa） Chain C 1–283（283 aa） Chain D 1–283（283 aa） Chain E 1–283（283 aa） Chain F 1–283（283 aa）	Not recorded	CLR CHOLESTEROL × 6	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.46 Å
9QN9 Connexin-32 (Cx32) gap junction channel in POPC-containing nanodiscs in the absence of CHS Deposited 2025-03-24	Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: 12-meric	Chain A 1–283（283 aa） Chain B 1–283（283 aa） Chain C 1–283（283 aa） Chain D 1–283（283 aa） Chain E 1–283（283 aa） Chain F 1–283（283 aa） Chain G 1–283（283 aa） Chain H 1–283（283 aa） Chain I 1–283（283 aa） Chain J 1–283（283 aa） Chain K 1–283（283 aa） Chain L 1–283（283 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.12 Å
9QND Connexin-32 (Cx32) W3S mutant gap junction channel in POPC-containing MSP2N2 nanodiscs Deposited 2025-03-24	Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: 12-meric	Chain A 1–283（283 aa） Chain B 1–283（283 aa） Chain C 1–283（283 aa） Chain D 1–283（283 aa） Chain E 1–283（283 aa） Chain F 1–283（283 aa） Chain G 1–283（283 aa） Chain H 1–283（283 aa） Chain I 1–283（283 aa） Chain J 1–283（283 aa） Chain K 1–283（283 aa） Chain L 1–283（283 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.35 Å
9QNF Connexin-32 (Cx32) in MSP2N2 nanodiscs with POPC Deposited 2025-03-25	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Insufficient information Homooligomer；Protein × 12 PDB declaration: 12-meric	Chain A 1–283（283 aa） Chain B 1–283（283 aa） Chain C 1–283（283 aa） Chain D 1–283（283 aa） Chain E 1–283（283 aa） Chain F 1–283（283 aa） Chain G 1–283（283 aa） Chain H 1–283（283 aa） Chain I 1–283（283 aa） Chain J 1–283（283 aa） Chain K 1–283（283 aa） Chain L 1–283（283 aa）	Not recorded	POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 12 CLR CHOLESTEROL × 12	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.16 Å
9QNT Connexin-32 (Cx32) in MSP2N2 nanodiscs with liver polar lipids Deposited 2025-03-25	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Insufficient information Homooligomer；Protein × 12 PDB declaration: 12-meric	Chain A 1–283（283 aa） Chain B 1–283（283 aa） Chain C 1–283（283 aa） Chain D 1–283（283 aa） Chain E 1–283（283 aa） Chain F 1–283（283 aa） Chain G 1–283（283 aa） Chain H 1–283（283 aa） Chain I 1–283（283 aa） Chain J 1–283（283 aa） Chain K 1–283（283 aa） Chain L 1–283（283 aa）	Not recorded	POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 12 CLR CHOLESTEROL × 12	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.29 Å

14 other PDB entries and 14 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	CXB1_HUMAN
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–283； UniProt 1–283 Author chain B； PDBConstruct 1–283； UniProt 1–283 Author chain C； PDBConstruct 1–283； UniProt 1–283 Author chain D； PDBConstruct 1–283； UniProt 1–283 Author chain E； PDBConstruct 1–283； UniProt 1–283 Author chain F； PDBConstruct 1–283； UniProt 1–283 Author chain G； PDBConstruct 1–283； UniProt 1–283 Author chain H； PDBConstruct 1–283； UniProt 1–283 Author chain I； PDBConstruct 1–283； UniProt 1–283 Author chain J； PDBConstruct 1–283； UniProt 1–283 Author chain K； PDBConstruct 1–283； UniProt 1–283 Author chain L； PDBConstruct 1–283； UniProt 1–283

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	8qjh
Deposition date deposition_date	2023-09-13
Structure title title	Connexin-32 gap junction channel in complex with mefloquine
Keywords keywords	complex, MEMBRANE PROTEIN; MEMBRANE PROTEIN
Experimental Method method	ELECTRON MICROSCOPY

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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier	44.79 Å
Radius of gyration Rg (electron density) rg_electron	45.62 Å
Forward intensity I(0) i0	657918000.00
Molecular weight molecular_weight	235570.0 kDa
Excluded volume excluded_volume	303950 Å³
Envelope volume envelope_volume	391580 Å³
Hydration-shell volume shell_volume	72647 Å³
Envelope diameter envelope_diameter	154.3 Å
Shell Rg shell_rg	48.70 Å
Envelope Rg envelope_rg	46.13 Å
Shape Rg shape_rg	45.66 Å
Total Rg total_rg	45.57 Å
Total atoms total_atoms	16584
Residues n_residues	2076
Spherical-harmonic order n_harmonics	20
q range q_range	— – 0.5000 Å⁻¹
Data points n_points	101
Shell type shell_type	directional
Solvent electron density solvent_density	0.3340 e/Å³
Shell contrast contrast_shell	0.0300 e/Å³
CRYSOL version crysol_version	4.1.3

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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax	155.7 Å
Rg (real space) rg_real	45.01 Å
Rg uncertainty (real space) rg_real_error	2.11 Å
I(0) (real space) i0_real	6.5790e+08
I(0) uncertainty (real space) i0_real_error	1.3870e+07
Rg (reciprocal space) rg_reciprocal	44.80 Å
I(0) (reciprocal space) i0_reciprocal	657800000.0000
Solution quality estimate total_estimate	0.8249
Solution quality rating solution_quality	GOOD a GOOD solution
P(r) peaks n_peaks	2
Primary peak position r_peak_primary	48.9 Å
Skewness Skewness skewness	0.528
Kurtosis Kurtosis kurtosis	-0.183
Angular range angular_range	— – 0.1750 Å⁻¹
Current regularization parameter α current_alpha	0.0000
Highest regularization parameter α highest_alpha	89440000.0000
Real-space data points n_real_points	36
GNOM version gnom_version	4.1.3
Quality Criteria quality_criteria	AN1: 0.000; Oscil: 0.637; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.975; Smooth: 0.833

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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (1)

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8. Citations (1)

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Unit-cell length a length_a	1.000 Å
Unit-cell length b length_b	1.000 Å
Unit-cell length c length_c	1.000 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	—

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	—

Resolution resolution	2.91 Å
Resolution测定方法 resolution_method	—
颗粒数 num_particles	56469

Description description	Gap junction beta-1 protein
Molecular weight formula_weight	32065.5 kDa
Source method src_method	man
Copy count entity_count	12

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B,C,D,E,F,G,H,I,J,K,L
Atom count atom_count	16584

Connexin-32 gap junction channel in complex with mefloquine

1. Protein Identity and Related Structures Protein Identity & Related Structures

Gap junction beta-1 protein

State in the Current Structure

Other States of the Same Protein in the Database

SAXS scattering curve SAXS Profile

P(r) Distance Distribution P(r) Distribution

2. Structure Basics 2. Structure Basics

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

5. Crystallography and Experiment 5. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

冷冻电镜 EM 3D

6. Entities and Polymers Entities & Polymers (1)

Entity 1 Entity 1 polymer

Polymer Polymer

来源 Source

8. Citations (1)

9. Files and Curves (10)

Files (7)

Curves (3)

来源生物 organism	Homo sapiens
通用名 common_name	human
NCBI Class编号 ncbi_taxonomy_id	9606
宿主生物 host_organism	Homo sapiens

fit	/app/data/saxs_computation/qj/8qjh/8qjh.fit
pddf	/app/data/pr_computation/qj/8qjh/8qjh.pr.out
profile	/app/data/saxs_computation/qj/8qjh/8qjh.dat