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9qnt

Connexin-32 (Cx32) in MSP2N2 nanodiscs with liver polar lipids

Method: ELECTRON MICROSCOPY D_max: 150.5 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9qnt
deposition_date	2025-03-25
title	Connexin-32 (Cx32) in MSP2N2 nanodiscs with liver polar lipids
keywords	Gap junction channel, membrane transport, lipids, MEMBRANE PROTEIN; MEMBRANE PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	48.52 Å
rg_electron	49.11 Å
i0	864153000.00
molecular_weight	281440.0 kDa
excluded_volume	367200 Å³
envelope_volume	468840 Å³
shell_volume	81429 Å³
envelope_diameter	157.9 Å
shell_rg	51.24 Å
envelope_rg	48.68 Å
shape_rg	49.11 Å
total_rg	49.21 Å
total_atoms	19788
n_residues	2352
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	150.5 Å
rg_real	48.88 Å
rg_real_error	1.05 Å
i0_real	8.6420e+08
i0_real_error	1.6340e+07
rg_reciprocal	48.52 Å
i0_reciprocal	863700000.0000
total_estimate	0.7959
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	47.0 Å
skewness	0.516
kurtosis	-0.407
angular_range	— – 0.1600 Å⁻¹
current_alpha	0.0000
highest_alpha	225800000.0000
n_real_points	33
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.781; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.997; Smooth: 0.002

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	Gap junction beta-1 protein,Green fluorescent protein
formula_weight	63327.4 kDa
src_method	man
entity_count	12

Polymer

type	polypeptide(L)
strand_id	A,B,C,D,E,F,G,H,I,J,K,L
atom_count	19788

MNWTGLYTLLSGVNRHSTAIGRVWLSVIFIFRIMVLVVAAESVWGDEKSSFICNTLQPGCNSVCYDQFFPISHVRLWSLQLILVSTPALLVAMHVAHQQHIEKKMLRLEGHGDPLHLEEVKRHKVHISGTLWWTYVISVVFRLLFEAVFMYVFYLLYPGYAMVRLVKCDVYPCPNTVDCFVSRPTEKTVFTVFMLAASGICIILNVAEVVYLIIRACARRAQRRSNPPSRKGSGFGHRLSPEYKQNEINKLLSEQDGSLKDILRRSPGTGAGLAEKSDRCSACAAALEVLFQGPGGVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKAASAWSHPQFEKGGGSGGGSGGSAWSHPQFEK

Source

organism	Aequorea victoria
ncbi_taxonomy_id	6100
host_organism	Homo sapiens

Entity 2 non-polymer

description	(2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate
formula_weight	760.1 kDa
src_method	syn
entity_count	12

Entity 3 non-polymer

description	CHOLESTEROL
formula_weight	386.7 kDa
src_method	syn
entity_count	12

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	3.29 Å
resolution_method	—
num_particles	53450

fit	/app/data/saxs_computation/qn/9qnt/9qnt.fit
pddf	/app/data/pr_computation/qn/9qnt/9qnt.pr.out
profile	/app/data/saxs_computation/qn/9qnt/9qnt.dat