SAXSdb — 8qki

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Gap junction beta-1 protein

Homo sapiens

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein homooligomer Homooligomer Protein × 6 PDB declaration: hexameric（6） Consistent with protein copy count	Chain A； UniProt 1–283 Chain B； UniProt 1–283 Chain C； UniProt 1–283 Chain D； UniProt 1–283 Chain E； UniProt 1–283 Chain F； UniProt 1–283	Not recorded	CLR CHOLESTEROL × 6	ELECTRON MICROSCOPY cryo-EM buffer：pH 8 cryo-EM vitrification conditions：Cryogen ETHANE	Resolution 3.46 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 8QKI	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
5KK9 Connexin 32 G12R N-Terminal Mutant, Deposited 2016-06-21	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–22（22 aa）	Mutation：G12R Non-standard monomer：Yes (specific site not provided by mmCIF)	No recorded non-water small molecule	SOLUTION NMR NMR measurement conditions pH 7；288 K；Ionic strength (raw mmCIF value) 0.1；Pressure 1 NMR sample composition 1.0 mM ACE-MET-ASN-TRP-THR-GLY-LEU-TYR-THR-LEU-LEU-SER-ARG-VAL-ASN-ARG-HIS-SER-THR-ALA-ILE-GLY-ARG, 100 mM KCl, 100 uM TSP, 90% H2O/10% D2O \| 90% H2O/10% D2O	Resolution not provided
7ZXM cryo-EM structure of Connexin 32 gap junction channel Deposited 2022-05-21	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: dodecameric	Chain A 1–283（283 aa） Chain B 1–283（283 aa） Chain C 1–283（283 aa） Chain D 1–283（283 aa） Chain E 1–283（283 aa） Chain F 1–283（283 aa） Chain G 1–283（283 aa） Chain H 1–283（283 aa） Chain I 1–283（283 aa） Chain J 1–283（283 aa） Chain K 1–283（283 aa） Chain L 1–283（283 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.14 Å
7ZXN cryo-EM structure of Connexin 32 gap junction channel Deposited 2022-05-21	Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric	Chain A 1–283（283 aa） Chain B 1–283（283 aa） Chain C 1–283（283 aa） Chain D 1–283（283 aa） Chain E 1–283（283 aa） Chain F 1–283（283 aa）	Not recorded	CLR CHOLESTEROL × 6	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.06 Å
7ZXO cryo-EM structure of Connexin 32 gap junction channel Deposited 2022-05-21	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: dodecameric	Chain A 1–283（283 aa） Chain B 1–283（283 aa） Chain C 1–283（283 aa） Chain D 1–283（283 aa） Chain E 1–283（283 aa） Chain F 1–283（283 aa） Chain G 1–283（283 aa） Chain H 1–283（283 aa） Chain I 1–283（283 aa） Chain J 1–283（283 aa） Chain K 1–283（283 aa） Chain L 1–283（283 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.50 Å
7ZXP cryo-EM structure of Connexin 32 R22G mutation gap junction channel Deposited 2022-05-22	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: dodecameric	Chain A 1–283（283 aa） Chain B 1–283（283 aa） Chain C 1–283（283 aa） Chain D 1–283（283 aa） Chain E 1–283（283 aa） Chain F 1–283（283 aa） Chain G 1–283（283 aa） Chain H 1–283（283 aa） Chain I 1–283（283 aa） Chain J 1–283（283 aa） Chain K 1–283（283 aa） Chain L 1–283（283 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.39 Å
7ZXQ cryo-EM structure of Connexin 32 R22G mutation hemi channel Deposited 2022-05-22	Different ligand/ion Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric	Chain A 1–283（283 aa） Chain B 1–283（283 aa） Chain C 1–283（283 aa） Chain D 1–283（283 aa） Chain E 1–283（283 aa） Chain F 1–283（283 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.53 Å
7ZXT cryo-EM structure of Connexin 32 W3S mutation hemi channel Deposited 2022-05-22	Different ligand/ion Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric	Chain A 1–283（283 aa） Chain B 1–283（283 aa） Chain C 1–283（283 aa） Chain D 1–283（283 aa） Chain E 1–283（283 aa） Chain F 1–283（283 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
8QJF Connexin-32 gap junction channel in complex with 2-aminoethoxydiphenyl borate Deposited 2023-09-13	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: 12-meric	Chain A 1–283（283 aa） Chain C 1–283（283 aa） Chain D 1–283（283 aa） Chain E 1–283（283 aa） Chain F 1–283（283 aa） Chain G 1–283（283 aa） Chain H 1–283（283 aa） Chain I 1–283（283 aa） Chain J 1–283（283 aa） Chain K 1–283（283 aa） Chain L 1–283（283 aa） Chain M 1–283（283 aa）	Not recorded	CLR CHOLESTEROL × 12	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.86 Å
8QJH Connexin-32 gap junction channel in complex with mefloquine Deposited 2023-09-13	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: 12-meric	Chain A 1–283（283 aa） Chain B 1–283（283 aa） Chain C 1–283（283 aa） Chain D 1–283（283 aa） Chain E 1–283（283 aa） Chain F 1–283（283 aa） Chain G 1–283（283 aa） Chain H 1–283（283 aa） Chain I 1–283（283 aa） Chain J 1–283（283 aa） Chain K 1–283（283 aa） Chain L 1–283（283 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.91 Å
8QK6 Connexin-32 hemichannel upon addition of 2-aminoetoxydiphenyl borate Deposited 2023-09-14	Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric	Chain A 1–283（283 aa） Chain B 1–283（283 aa） Chain C 1–283（283 aa） Chain D 1–283（283 aa） Chain E 1–283（283 aa） Chain F 1–283（283 aa）	Not recorded	CLR CHOLESTEROL × 6	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.18 Å
9QN9 Connexin-32 (Cx32) gap junction channel in POPC-containing nanodiscs in the absence of CHS Deposited 2025-03-24	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: 12-meric	Chain A 1–283（283 aa） Chain B 1–283（283 aa） Chain C 1–283（283 aa） Chain D 1–283（283 aa） Chain E 1–283（283 aa） Chain F 1–283（283 aa） Chain G 1–283（283 aa） Chain H 1–283（283 aa） Chain I 1–283（283 aa） Chain J 1–283（283 aa） Chain K 1–283（283 aa） Chain L 1–283（283 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.12 Å
9QND Connexin-32 (Cx32) W3S mutant gap junction channel in POPC-containing MSP2N2 nanodiscs Deposited 2025-03-24	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: 12-meric	Chain A 1–283（283 aa） Chain B 1–283（283 aa） Chain C 1–283（283 aa） Chain D 1–283（283 aa） Chain E 1–283（283 aa） Chain F 1–283（283 aa） Chain G 1–283（283 aa） Chain H 1–283（283 aa） Chain I 1–283（283 aa） Chain J 1–283（283 aa） Chain K 1–283（283 aa） Chain L 1–283（283 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.35 Å
9QNF Connexin-32 (Cx32) in MSP2N2 nanodiscs with POPC Deposited 2025-03-25	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Insufficient information Homooligomer；Protein × 12 PDB declaration: 12-meric	Chain A 1–283（283 aa） Chain B 1–283（283 aa） Chain C 1–283（283 aa） Chain D 1–283（283 aa） Chain E 1–283（283 aa） Chain F 1–283（283 aa） Chain G 1–283（283 aa） Chain H 1–283（283 aa） Chain I 1–283（283 aa） Chain J 1–283（283 aa） Chain K 1–283（283 aa） Chain L 1–283（283 aa）	Not recorded	POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 12 CLR CHOLESTEROL × 12	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.16 Å
9QNT Connexin-32 (Cx32) in MSP2N2 nanodiscs with liver polar lipids Deposited 2025-03-25	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Insufficient information Homooligomer；Protein × 12 PDB declaration: 12-meric	Chain A 1–283（283 aa） Chain B 1–283（283 aa） Chain C 1–283（283 aa） Chain D 1–283（283 aa） Chain E 1–283（283 aa） Chain F 1–283（283 aa） Chain G 1–283（283 aa） Chain H 1–283（283 aa） Chain I 1–283（283 aa） Chain J 1–283（283 aa） Chain K 1–283（283 aa） Chain L 1–283（283 aa）	Not recorded	POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 12 CLR CHOLESTEROL × 12	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.29 Å

14 other PDB entries and 14 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	CXB1_HUMAN
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–283； UniProt 1–283 Author chain B； PDBConstruct 1–283； UniProt 1–283 Author chain C； PDBConstruct 1–283； UniProt 1–283 Author chain D； PDBConstruct 1–283； UniProt 1–283 Author chain E； PDBConstruct 1–283； UniProt 1–283 Author chain F； PDBConstruct 1–283； UniProt 1–283

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	8qki
Deposition date deposition_date	2023-09-15
Structure title title	Connexin-32 hemichannel upon addition of mefloquine
Keywords keywords	connexin, hemichannel, mefloqine, MEMBRANE PROTEIN; MEMBRANE PROTEIN
Experimental Method method	ELECTRON MICROSCOPY

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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier	34.24 Å
Radius of gyration Rg (electron density) rg_electron	32.84 Å
Forward intensity I(0) i0	266084000.00
Molecular weight molecular_weight	144730.0 kDa
Excluded volume excluded_volume	187160 Å³
Envelope volume envelope_volume	239950 Å³
Hydration-shell volume shell_volume	57860 Å³
Envelope diameter envelope_diameter	100.4 Å
Shell Rg shell_rg	42.00 Å
Envelope Rg envelope_rg	32.31 Å
Shape Rg shape_rg	32.84 Å
Total Rg total_rg	33.66 Å
Total atoms total_atoms	10188
Residues n_residues	1242
Spherical-harmonic order n_harmonics	20
q range q_range	— – 0.5000 Å⁻¹
Data points n_points	101
Shell type shell_type	directional
Solvent electron density solvent_density	0.3340 e/Å³
Shell contrast contrast_shell	0.0300 e/Å³
CRYSOL version crysol_version	4.1.3

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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax	98.1 Å
Rg (real space) rg_real	33.97 Å
Rg uncertainty (real space) rg_real_error	0.37 Å
I(0) (real space) i0_real	2.6610e+08
I(0) uncertainty (real space) i0_real_error	3.6940e+06
Rg (reciprocal space) rg_reciprocal	34.14 Å
I(0) (reciprocal space) i0_reciprocal	266100000.0000
Solution quality estimate total_estimate	0.6872
Solution quality rating solution_quality	REASONABLE a REASONABLE solution
P(r) peaks n_peaks	1
Primary peak position r_peak_primary	46.9 Å
Skewness Skewness skewness	-0.039
Kurtosis Kurtosis kurtosis	-0.512
Angular range angular_range	— – 0.2300 Å⁻¹
Current regularization parameter α current_alpha	0.0000
Highest regularization parameter α highest_alpha	26960000.0000
Real-space data points n_real_points	47
GNOM version gnom_version	4.1.3
Quality Criteria quality_criteria	AN1: 0.000; Oscil: 0.949; Stabil: 1.000; Sysdev: 0.122; Positv: 1.000; Valcen: 0.970; Smooth: 0.745

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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (2)

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8. Citations (1)

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Resolution resolution	3.46 Å
Resolution测定方法 resolution_method	—
颗粒数 num_particles	108632

Description description	Gap junction beta-1 protein
Molecular weight formula_weight	32065.5 kDa
Source method src_method	man
Copy count entity_count	6

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B,C,D,E,F
Atom count atom_count	10188

来源生物 organism	Homo sapiens
通用名 common_name	human
NCBI Class编号 ncbi_taxonomy_id	9606
宿主生物 host_organism	Homo sapiens

Description description	CHOLESTEROL
Molecular weight formula_weight	386.7 kDa
Source method src_method	syn
Copy count entity_count	6

Connexin-32 hemichannel upon addition of mefloquine

1. Protein Identity and Related Structures Protein Identity & Related Structures

Gap junction beta-1 protein

State in the Current Structure

Other States of the Same Protein in the Database

SAXS scattering curve SAXS Profile

P(r) Distance Distribution P(r) Distribution

2. Structure Basics 2. Structure Basics

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

5. Crystallography and Experiment 5. Crystallography & Experiment

冷冻电镜 EM 3D

6. Entities and Polymers Entities & Polymers (2)

Entity 1 Entity 1 polymer

Polymer Polymer

来源 Source

Entity 2 Entity 2 non-polymer

8. Citations (1)

9. Files and Curves (10)

Files (7)

Curves (3)

fit	/app/data/saxs_computation/qk/8qki/8qki.fit
pddf	/app/data/pr_computation/qk/8qki/8qki.pr.out
profile	/app/data/saxs_computation/qk/8qki/8qki.dat