8t4y

Human HCN1 F186C S264C C309A bound to cAMP, reconstituted in LMNG + SPL

Method: ELECTRON MICROSCOPY D_max: 134.2 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	8t4y
沉积日期 deposition_date	2023-06-12
结构标题 title	Human HCN1 F186C S264C C309A bound to cAMP, reconstituted in LMNG + SPL
关键词 keywords	Membrane Protein, Ion Channel, TRANSPORT PROTEIN; TRANSPORT PROTEIN
实验方法 method	ELECTRON MICROSCOPY

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	42.24 Å
回转半径 Rg (电子) rg_electron	41.30 Å
零角强度 I(0) i0	590965000.00
分子量 molecular_weight	188410.0 kDa
排除体积 excluded_volume	230470 Å³
包络体积 envelope_volume	374140 Å³
水化壳体积 shell_volume	74088 Å³
包络直径 envelope_diameter	134.7 Å
壳层 Rg shell_rg	48.45 Å
包络 Rg envelope_rg	40.34 Å
形状 Rg shape_rg	41.38 Å
总 Rg total_rg	41.42 Å
总原子数 total_atoms	13348
残基数 n_residues	2004
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	134.2 Å
Rg (实空间) rg_real	42.05 Å
Rg 误差 (实空间) rg_real_error	0.99 Å
I(0) (实空间) i0_real	5.9100e+08
I(0) 误差 (实空间) i0_real_error	9.0900e+06
Rg (倒空间) rg_reciprocal	42.24 Å
I(0) (倒空间) i0_reciprocal	591100000.0000
解质量估计 total_estimate	0.8881
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	1
主峰位置 r_peak_primary	54.4 Å
偏度 Skewness skewness	0.171
峰度 Kurtosis kurtosis	-0.454
角度范围 angular_range	— – 0.1850 Å⁻¹
当前正则化参数 α current_alpha	0.0000
最高正则化参数 α highest_alpha	56790000.0000
实空间数据点数 n_real_points	38
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.883; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.974; Smooth: 0.918

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (2)

Entity 1 polymer

描述 description	Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
分子量 formula_weight	98839.8 kDa
突变 mutation	F186C, S264C, C309A
来源方法 src_method	man
拷贝数 entity_count	4

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A,D,C,B
原子数 atom_count	13348

MEGGGKPNSSSNSRDDGNSVFPAKASATGAGPAAAEKRLGTPPGGGGAGAKEHGNSVCFKVDGGGGGGGGGGGGEEPAGGFEDAEGPRRQYGFMQRQFTSMLQPGVNKFSLRMFGSQKAVEKEQERVKTAGFWIIHPYSDFRFYWDLIMLIMMVGNLVIIPVGITFFTEQTTTPWIIFNVASDTVCLLDLIMNFRTGTVNEDSSEIILDPKVIKMNYLKSWFVVDFISSIPVDYIFLIVEKGMDSEVYKTARALRIVRFTKILCLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVVRIFNLIGMMLLLAHWDGCLQFLVPLLQDFPPDCWVSLNEMVNDSWGKQYSYALFKAMSHMLCIGYGAQAPVSMSDLWITMLSMIVGATCYAMFVGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPADMRQKIHDYYEHRYQGKIFDEENILNELNDPLREEIVNFNCRKLVATMPLFANADPNFVTAMLSKLRFEVFQPGDYIIREGAVGKKMYFIQHGVAGVITKSSKEMKLTDGSYFGEICLLTKGRRTASVRADTYCRLYSLSVDNFNEVLEEYPMMRRAFETVAIDRLDRIGKKNSILLQKFQKDLNTGVFNNQENEILKQIVKHDREMVQAIAPINYPQMTTLNSTSSTTTPTSRMRTQSPPVYTATSLSHSNLHSPSPSTQTPQPSAILSPCSYTTAVCSPPVQSPLAARTFHYASPTASQLSLMQQQPQQQVQQSQPPQTQPQQPSPQPQTPGSSTPKNEVHKSTQALHNTNLTREVRPLSASQPSLPHEVSTLISRPHPTVGESLASIPQPVTAVPGTGLQAGGRSTVPQRVTLFRQMSSGAIPPNRGVPPAPPPPAAALPRESSSVLNTDPDAEKPRFASNL

来源 Source

来源生物 organism	Homo sapiens
通用名 common_name	human
NCBI 分类编号 ncbi_taxonomy_id	9606
宿主生物 host_organism	Homo sapiens

Entity 2 non-polymer

描述 description	ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE
分子量 formula_weight	329.2 kDa
来源方法 src_method	syn
拷贝数 entity_count	4

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7. 引用文献 (1)

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分辨率 resolution	3.58 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	122422

fit	/app/data/saxs_computation/t4/8t4y/8t4y.fit
pddf	/app/data/pr_computation/t4/8t4y/8t4y.pr.out
profile	/app/data/saxs_computation/t4/8t4y/8t4y.dat