Structure of the human chimera HCN112 hyperpolarization-activated cyclic nucleotide-gated ion channel in complex with cAMP.

Method: ELECTRON MICROSCOPY D_max: 122.3 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9r1t
deposition_date	2025-04-28
title	Structure of the human chimera HCN112 hyperpolarization-activated cyclic nucleotide-gated ion channel in complex with cAMP.
keywords	Ion channel, HCN channel, pacemaker channel, MEMBRANE PROTEIN; MEMBRANE PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	40.45 Å
rg_electron	39.96 Å
i0	695536000.00
molecular_weight	224090.0 kDa
excluded_volume	283010 Å³
envelope_volume	376160 Å³
shell_volume	74918 Å³
envelope_diameter	123.7 Å
shell_rg	48.45 Å
envelope_rg	39.41 Å
shape_rg	39.98 Å
total_rg	40.33 Å
total_atoms	15784
n_residues	2016
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	122.3 Å
rg_real	40.23 Å
rg_real_error	0.65 Å
i0_real	6.9550e+08
i0_real_error	1.1120e+07
rg_reciprocal	40.45 Å
i0_reciprocal	695700000.0000
total_estimate	0.8860
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	50.2 Å
skewness	0.101
kurtosis	-0.473
angular_range	— – 0.1950 Å⁻¹
current_alpha	0.0000
highest_alpha	45850000.0000
n_real_points	40
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.919; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.969; Smooth: 0.787

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4. Crystallography & Experiment

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5. Entities & Polymer Info (2)

Entity 1 polymer

description	;Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1,Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2,Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1,Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2,HCN112 holo, in complex with cAMP ;
formula_weight	72852.2 kDa
src_method	man
entity_count	4

Polymer

type	polypeptide(L)
strand_id	A,B,C,D
atom_count	15784

MEGGGKPNSSSNSRDDGNSVFPAKASATGAGPAAAEKRLGTPPGGGGAGAKEHGNSVCFKVDGGGGGGGGGGGGEEPAGGFEDAEGPRRQYGFMQRQFTSMLQPGVNKFSLRMFGSQKAVEKEQERVKTAGFWIIHPYSDFRFYWDLIMLIMMVGNLVIIPVGITFFTEQTTTPWIIFNVASDTVFLLDLIMNFRTGTVNEDSSEIILDPKVIKMNYLKSWFVVDFISSIPVDYIFLIVEKGMDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVVRIFNLIGMMLLLCHWDGCLQFLVPLLQDFPPDCWVSLNEMVNDSWGKQYSYALFKAMSHMLCIGYGAQAPVSMSDLWITMLSMIVGATCYAMFVGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPADFRQKIHDYYEHRYQGKMFDEDSILGELNGPLREEIVNFNCRKLVASMPLFANADPNFVTAMLTKLKFEVFQPGDYIIREGTIGKKMYFIQHGVVSVLTKGNKEMKLSDGSYFGEICLLTRGRRTASVRADTYCRLYSLSVDNFNEVLEEYPMMRRAFETVAIDRLDRIGKKNSILLHKVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

Source

organism	Homo sapiens
ncbi_taxonomy_id	9606
host_organism	Homo sapiens

Entity 2 non-polymer

description	ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE
formula_weight	329.2 kDa
src_method	syn
entity_count	4

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	3.34 Å
resolution_method	—
num_particles	97137

fit	/app/data/saxs_computation/r1/9r1t/9r1t.fit
pddf	/app/data/pr_computation/r1/9r1t/9r1t.pr.out
profile	/app/data/saxs_computation/r1/9r1t/9r1t.dat