Human apo HCN1 nanodisc

Method: ELECTRON MICROSCOPY D_max: 127.1 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9pxn
deposition_date	2025-08-06
title	Human apo HCN1 nanodisc
keywords	ion channel, pacemaker channel, hyperpolarization-activated, MEMBRANE PROTEIN; MEMBRANE PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	41.38 Å
rg_electron	41.04 Å
i0	672165000.00
molecular_weight	223230.0 kDa
excluded_volume	283430 Å³
envelope_volume	401400 Å³
shell_volume	77687 Å³
envelope_diameter	128.9 Å
shell_rg	49.48 Å
envelope_rg	40.46 Å
shape_rg	41.00 Å
total_rg	41.61 Å
total_atoms	31460
n_residues	1916
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	127.1 Å
rg_real	41.14 Å
rg_real_error	0.86 Å
i0_real	6.7220e+08
i0_real_error	1.0780e+07
rg_reciprocal	41.38 Å
i0_reciprocal	672300000.0000
total_estimate	0.8900
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	53.5 Å
skewness	0.085
kurtosis	-0.470
angular_range	— – 0.1900 Å⁻¹
current_alpha	0.0000
highest_alpha	55690000.0000
n_real_points	39
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.898; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.962; Smooth: 0.909

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4. Crystallography & Experiment

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5. Entities & Polymer Info (1)

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	2.500 Å
resolution_low	— Å
r_factor	—
r_work	—
r_free	—

resolution	2.50 Å
resolution_method	—
num_particles	236378

description	Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1,Thermostable Green Protein
formula_weight	103035.6 kDa
src_method	man
entity_count	4

Human apo HCN1 nanodisc

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

EM 3D

5. Entities & Polymer Info (1)

Entity 1 polymer

Polymer

Source

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)

organism	synthetic construct
ncbi_taxonomy_id	32630
host_organism	Komagataella pastoris

fit	/app/data/saxs_computation/px/9pxn/9pxn.fit
pddf	/app/data/pr_computation/px/9pxn/9pxn.pr.out
profile	/app/data/saxs_computation/px/9pxn/9pxn.dat

type	polypeptide(L)
strand_id	B,A,C,D
atom_count	31460