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8xbw

The cryo-EM structure of the RAD51 N-terminal lobe domain bound to the histone H4 tail of the nucleosome

Method: ELECTRON MICROSCOPY
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Histone H3.1

Homo sapiens

UniProt P68431

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–DNA Heteromer Protein 3 DNA 2 Histone H4 × 1 （P62805） ;DNA (5'-D(P*AP*CP*CP*GP*CP*TP*TP*AP*AP*AP*CP*GP*CP*AP*CP*GP*TP*A)-3') ; × 1 ;DNA (5'-D(P*TP*AP*CP*GP*TP*GP*CP*GP*TP*TP*TP*AP*AP*GP*CP*GP*GP*T)-3') ; × 1 DNA repair protein RAD51 homolog 1 × 1 （Q06609） Consistent with all polymers

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
2b2t 2b2u 2b2v 2b2w 2cv5 2l75 2ri7 2uxn 3a1b 3afa 3ayw 3aze

476 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name H31_HUMAN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain E； PDBConstruct 4–139； UniProt 1–136

Histone H4

Homo sapiens

UniProt P62805

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–DNA Heteromer Protein 3 DNA 2 Histone H3.1 × 1 （P68431） ;DNA (5'-D(P*AP*CP*CP*GP*CP*TP*TP*AP*AP*AP*CP*GP*CP*AP*CP*GP*TP*A)-3') ; × 1 ;DNA (5'-D(P*TP*AP*CP*GP*TP*GP*CP*GP*TP*TP*TP*AP*AP*GP*CP*GP*GP*T)-3') ; × 1 DNA repair protein RAD51 homolog 1 × 1 （Q06609） Consistent with all polymers

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
10xz 10ya 10yb 10yc 10yd 10ye 10yf 10yg 10yh 10yi 2bqz 2bqz

634 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name H4_HUMAN
Isoform —
PDB entities 2
Chains and sequence ranges Author chain F； PDBConstruct 4–106； UniProt 1–103

DNA repair protein RAD51 homolog 1

Homo sapiens

UniProt Q06609

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–DNA Heteromer Protein 3 DNA 2 Histone H3.1 × 1 （P68431） Histone H4 × 1 （P62805） ;DNA (5'-D(P*AP*CP*CP*GP*CP*TP*TP*AP*AP*AP*CP*GP*CP*AP*CP*GP*TP*A)-3') ; × 1 ;DNA (5'-D(P*TP*AP*CP*GP*TP*GP*CP*GP*TP*TP*TP*AP*AP*GP*CP*GP*GP*T)-3') ; × 1 Consistent with all polymers

Other States of the Same Protein in the Database

Monomer
1b22

1 assemblies

Homodimer

None

Homotetramer

None

Other homooligomers
5jzc 5np7 5nwl 5nwl 8bsc 8gyk 9qnb

7 assemblies

Heteromeric or nucleic-acid complex
1n0w 5h1b 5h1c 7c9a 7ejc 7eje 8bq2 8br2 8jnd 8jne 8jnf 8pbc

44 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name RAD51_HUMAN
Isoform —
PDB entities 5
Chains and sequence ranges Author chain L； PDBConstruct 4–342； UniProt 1–339

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id8xbw
Deposition date deposition_date2023-12-07
Structure title titleThe cryo-EM structure of the RAD51 N-terminal lobe domain bound to the histone H4 tail of the nucleosome
Keywords keywordsNucleosome, Recombinase, DNA BINDING PROTEIN-DNA Complex; DNA BINDING PROTEIN/DNA
Experimental Method methodELECTRON MICROSCOPY
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

8xbw__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

8xbw__assembly_1__model_1 | I(q)

10-2 10-1 106 107 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

8xbw__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）24.14 Å
Rg (electron density)23.26 Å
Total Rg24.05 Å
Atom count2671
Residues278
Excluded volume45509 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 8xbw__assembly_1__model_1 pentameric （5） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (5)

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7. Citations (1)