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8z5r

human phosphorylase kinase alpha/gamma/delta subcomplex - phosphorylation and Ca2+ bound state

Method: ELECTRON MICROSCOPY Dmax: 130.4 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform

Homo sapiens

UniProt P46020

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 3 PDB declaration: trimeric（3） Consistent with protein copy count Chain A； UniProt 1–1223 Non-standard monomer：Yes (specific site not provided by mmCIF) Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform × 1 （Q16816） Calmodulin-3 × 1 （P0DP25） ATP ADENOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 6.8 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.52 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8Z5R Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
8JFK PhK holoenzyme in inactive state, muscle isoform Deposited 2023-05-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain A 1–1223（1223 aa）
Chain E 1–1223（1223 aa）
Chain I 1–1223（1223 aa）
Chain M 1–1223（1223 aa）
Not recorded FAR FARNESYL × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8JFL PhK holoenzyme in active state, muscle isoform Deposited 2023-05-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain A 1–1223（1223 aa）
Chain E 1–1223（1223 aa）
Chain I 1–1223（1223 aa）
Chain M 1–1223（1223 aa）
Not recorded FAR FARNESYL × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8.2
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8XY7 hPhK alpha-gamma subcomplex in active state Deposited 2024-01-19 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–1223（1223 aa）
Not recorded FAR FARNESYL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8.2
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8XYA hPhK alpha-beta-gamma-delta subcomplex in inactive state Deposited 2024-01-19 Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–1223（1223 aa）
Not recorded FAR FARNESYL × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
8XYB hPhK gamma-delta subcomplex in inactive state Deposited 2024-01-19 Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–1223（1223 aa）
Not recorded FAR FARNESYL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8Z5M human phosphorylase kinase alpha/gamma/delta subcomplex - inactive state Deposited 2024-04-18 Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–1223（1223 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.66 Å
8Z5P human phosphorylase kinase - inactive state Deposited 2024-04-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–1223（1223 aa）
Chain E 1–1223（1223 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.41 Å
8Z5Q human phosphorylase kinase - inactive state Deposited 2024-04-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain A 1–1223（1223 aa）
Chain E 1–1223（1223 aa）
Chain I 1–1223（1223 aa）
Chain M 1–1223（1223 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.24 Å
8Z5T human phosphorylase kinase - phosphorylation and Ca2+ bound state Deposited 2024-04-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain A 1–1223（1223 aa）
Chain E 1–1223（1223 aa）
Chain I 1–1223（1223 aa）
Chain M 1–1223（1223 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) ATP ADENOSINE-5'-TRIPHOSPHATE × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.74 Å

9 other PDB entries and 9 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name KPB1_HUMAN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–1223； UniProt 1–1223

Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform

Homo sapiens

UniProt Q16816

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 3 PDB declaration: trimeric（3） Consistent with protein copy count Chain C； UniProt 1–387 Not recorded Phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform × 1 （P46020） Calmodulin-3 × 1 （P0DP25） ATP ADENOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 6.8 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.52 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8Z5R Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
8JFK PhK holoenzyme in inactive state, muscle isoform Deposited 2023-05-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain C 1–387（387 aa）
Chain G 1–387（387 aa）
Chain K 1–387（387 aa）
Chain O 1–387（387 aa）
Not recorded FAR FARNESYL × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8JFL PhK holoenzyme in active state, muscle isoform Deposited 2023-05-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 1–387（387 aa）
Chain G 1–387（387 aa）
Chain K 1–387（387 aa）
Chain O 1–387（387 aa）
Not recorded FAR FARNESYL × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8.2
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8XY7 hPhK alpha-gamma subcomplex in active state Deposited 2024-01-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 1–387（387 aa）
Not recorded FAR FARNESYL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8.2
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8XYA hPhK alpha-beta-gamma-delta subcomplex in inactive state Deposited 2024-01-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain C 1–387（387 aa）
Not recorded FAR FARNESYL × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
8XYB hPhK gamma-delta subcomplex in inactive state Deposited 2024-01-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 1–387（387 aa）
Not recorded FAR FARNESYL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8Z5M human phosphorylase kinase alpha/gamma/delta subcomplex - inactive state Deposited 2024-04-18 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 1–387（387 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.66 Å
8Z5P human phosphorylase kinase - inactive state Deposited 2024-04-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain C 1–387（387 aa）
Chain G 1–387（387 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.41 Å
8Z5Q human phosphorylase kinase - inactive state Deposited 2024-04-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain C 1–387（387 aa）
Chain G 1–387（387 aa）
Chain K 1–387（387 aa）
Chain O 1–387（387 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.24 Å
8Z5T human phosphorylase kinase - phosphorylation and Ca2+ bound state Deposited 2024-04-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain C 1–387（387 aa）
Chain G 1–387（387 aa）
Chain K 1–387（387 aa）
Chain O 1–387（387 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.74 Å

9 other PDB entries and 9 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PHKG1_HUMAN
Isoform —
PDB entities 2
Chains and sequence ranges Author chain C； PDBConstruct 1–387； UniProt 1–387

Calmodulin-3

Homo sapiens

UniProt P0DP25

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 3 PDB declaration: trimeric（3） Consistent with protein copy count Chain D； UniProt 1–149 Not recorded Phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform × 1 （P46020） Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform × 1 （Q16816） ATP ADENOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 6.8 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.52 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8Z5R Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
7BYL Cryo-EM structure of human KCNQ4 Deposited 2020-04-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain B 1–149（149 aa）
Chain D 1–149（149 aa）
Chain F 1–149（149 aa）
Chain H 1–149（149 aa）
Not recorded PT5 [(2R)-1-octadecanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phospho ryl]oxy-propan-2-yl] (8Z)-icosa-5,8,11,14-tetraenoate × 4 K POTASSIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
7BYM Cryo-EM structure of human KCNQ4 with retigabine Deposited 2020-04-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain B 1–149（149 aa）
Chain D 1–149（149 aa）
Chain F 1–149（149 aa）
Chain H 1–149（149 aa）
Not recorded PT5 [(2R)-1-octadecanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phospho ryl]oxy-propan-2-yl] (8Z)-icosa-5,8,11,14-tetraenoate × 4 FBX ethyl N-[2-azanyl-4-[(4-fluorophenyl)methylamino]phenyl]carbamate × 4 K POTASSIUM ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7BYN Cryo-EM structure of human KCNQ4 with linopirdine Deposited 2020-04-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain B 1–149（149 aa）
Chain D 1–149（149 aa）
Chain F 1–149（149 aa）
Chain H 1–149（149 aa）
Not recorded PT5 [(2R)-1-octadecanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phospho ryl]oxy-propan-2-yl] (8Z)-icosa-5,8,11,14-tetraenoate × 4 K POTASSIUM ION × 3 FCC 1-phenyl-3,3-bis(pyridin-4-ylmethyl)indol-2-one × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7CR3 human KCNQ2-CaM in apo state Deposited 2020-08-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain C 1–149（149 aa）
Chain E 1–149（149 aa）
Chain F 1–149（149 aa）
Chain H 1–149（149 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7CR4 human KCNQ2-CaM in complex with ztz240 Deposited 2020-08-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain C 1–149（149 aa）
Chain E 1–149（149 aa）
Chain G 1–149（149 aa）
Chain H 1–149（149 aa）
Not recorded GB9 N-(6-chloranylpyridin-3-yl)-4-fluoranyl-benzamide × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
7CR7 human KCNQ2-CaM in complex with retigabine Deposited 2020-08-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain C 1–149（149 aa）
Chain E 1–149（149 aa）
Chain F 1–149（149 aa）
Chain H 1–149（149 aa）
Not recorded FBX ethyl N-[2-azanyl-4-[(4-fluorophenyl)methylamino]phenyl]carbamate × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
7VNP Structure of human KCNQ4-ML213 complex with PIP2 Deposited 2021-10-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain B 1–149（149 aa）
Chain D 1–149（149 aa）
Chain F 1–149（149 aa）
Chain H 1–149（149 aa）
Not recorded PT5 [(2R)-1-octadecanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phospho ryl]oxy-propan-2-yl] (8Z)-icosa-5,8,11,14-tetraenoate × 8 K POTASSIUM ION × 4 7YV (1S,2S,4R)-N-(2,4,6-trimethylphenyl)bicyclo[2.2.1]heptane-2-carboxamid × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.79 Å
7VNQ Structure of human KCNQ4-ML213 complex in nanodisc Deposited 2021-10-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain B 1–149（149 aa）
Chain D 1–149（149 aa）
Chain F 1–149（149 aa）
Chain H 1–149（149 aa）
Not recorded 7YV (1S,2S,4R)-N-(2,4,6-trimethylphenyl)bicyclo[2.2.1]heptane-2-carboxamid × 4 K POTASSIUM ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.96 Å
7VNR Structure of human KCNQ4-ML213 complex in digitonin Deposited 2021-10-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain B 1–149（149 aa）
Chain D 1–149（149 aa）
Chain F 1–149（149 aa）
Chain H 1–149（149 aa）
Not recorded 7YV (1S,2S,4R)-N-(2,4,6-trimethylphenyl)bicyclo[2.2.1]heptane-2-carboxamid × 4 K POTASSIUM ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
7XNI human KCNQ1-CaM in apo state Deposited 2022-04-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain C 1–149（149 aa）
Chain E 1–149（149 aa）
Chain F 1–149（149 aa）
Chain H 1–149（149 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
7XNK human KCNQ1-CaM in complex with ML277 Deposited 2022-04-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 1–149（149 aa）
Chain D 1–149（149 aa）
Chain F 1–149（149 aa）
Chain H 1–149（149 aa）
Not recorded K POTASSIUM ION × 4 I0S (2R)-N-[4-(4-methoxyphenyl)-1,3-thiazol-2-yl]-1-(4-methylbenzene-1-sulfonyl)piperidine-2-carboxamide × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å
7XNL human KCNQ1-CaM-ML277-PIP2 complex in state A Deposited 2022-04-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 1–149（149 aa）
Chain D 1–149（149 aa）
Chain F 1–149（149 aa）
Chain H 1–149（149 aa）
Not recorded K POTASSIUM ION × 4 I0S (2R)-N-[4-(4-methoxyphenyl)-1,3-thiazol-2-yl]-1-(4-methylbenzene-1-sulfonyl)piperidine-2-carboxamide × 4 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7XNN human KCNQ1-CaM-ML277-PIP2 complex in state B Deposited 2022-04-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–149（149 aa）
Chain D 1–149（149 aa）
Chain F 1–149（149 aa）
Chain H 1–149（149 aa）
Not recorded K POTASSIUM ION × 4 I0S (2R)-N-[4-(4-methoxyphenyl)-1,3-thiazol-2-yl]-1-(4-methylbenzene-1-sulfonyl)piperidine-2-carboxamide × 4 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8BD2 Calcium-bound Calmodulin variant G113R Deposited 2022-10-18 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 2–149（148 aa）
Not recorded CA CALCIUM ION × 2 SOLUTION NMR
NMR measurement conditions pH 6.69；298.1 K；Ionic strength (raw mmCIF value) 127；Pressure 1
NMR sample composition 100 mM potassium chloride, 5 mM calcium chloride, 2 mM sodium azide, 20 mM HEPES, 0.1 mM 2,2,3,3-tetradeutero TSP-d4, 1 mM (Ser81-Lys148-U-15N,13C) Calmodulin G113R, 95% H2O/5% D2O | 95% H2O/5% D2O
Resolution not provided
8Z5M human phosphorylase kinase alpha/gamma/delta subcomplex - inactive state Deposited 2024-04-18 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain D 1–149（149 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.66 Å
8Z5P human phosphorylase kinase - inactive state Deposited 2024-04-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain D 1–149（149 aa）
Chain H 1–149（149 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.41 Å
8Z5Q human phosphorylase kinase - inactive state Deposited 2024-04-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain D 1–149（149 aa）
Chain H 1–149（149 aa）
Chain L 1–149（149 aa）
Chain P 1–149（149 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.24 Å
8Z5T human phosphorylase kinase - phosphorylation and Ca2+ bound state Deposited 2024-04-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain D 1–149（149 aa）
Chain H 1–149（149 aa）
Chain L 1–149（149 aa）
Chain P 1–149（149 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.74 Å
9IXY Human KCNQ2-CaM-Ebio2 Complex in the Presence of PIP2 Deposited 2024-07-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain E 1–149（149 aa）
Chain F 1–149（149 aa）
Chain G 1–149（149 aa）
Chain H 1–149（149 aa）
Not recorded A1L3C ~{N}-[7-[bis(fluoranyl)methoxy]-1-prop-2-ynyl-indazol-3-yl]-3,3-dimethyl-butanamide × 4 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9LIZ Human KCNQ5-CaM in complex with PIP2 Deposited 2025-01-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain C 1–149（149 aa）
Chain E 1–149（149 aa）
Chain F 1–149（149 aa）
Chain H 1–149（149 aa）
Not recorded PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9LJ1 Human KCNQ5-CaM-PIP2-HN37 complex in a closed conformation. Deposited 2025-01-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain C 1–149（149 aa）
Chain E 1–149（149 aa）
Chain F 1–149（149 aa）
Chain H 1–149（149 aa）
Not recorded 9MF methyl N-[4-[(4-fluorophenyl)methyl-prop-2-ynyl-amino]-2,6-dimethyl-phenyl]carbamate × 4 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
9LJ5 Human KCNQ5-CaM-PIP2-HN37 complex in an open conformation. Deposited 2025-01-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain C 1–149（149 aa）
Chain E 1–149（149 aa）
Chain F 1–149（149 aa）
Chain H 1–149（149 aa）
Not recorded PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 8 9MF methyl N-[4-[(4-fluorophenyl)methyl-prop-2-ynyl-amino]-2,6-dimethyl-phenyl]carbamate × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å

22 other PDB entries and 22 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name CALM3_HUMAN
Isoform —
PDB entities 3
Chains and sequence ranges Author chain D； PDBConstruct 20–168； UniProt 1–149

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 8z5r

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 8z5r
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2. Structure Basics 2. Structure Basics

Entry ID entry_id8z5r
Deposition date deposition_date2024-04-18
Structure title titlehuman phosphorylase kinase alpha/gamma/delta subcomplex - phosphorylation and Ca2+ bound state
Keywords keywordsKinase, glycogenolysis, CYTOSOLIC PROTEIN; CYTOSOLIC PROTEIN
Experimental Method methodELECTRON MICROSCOPY
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier38.91 Å
Radius of gyration Rg (electron density) rg_electron38.19 Å
Forward intensity I(0) i0388973000.00
Molecular weight molecular_weight160350.0 kDa
Excluded volume excluded_volume200780 ų
Envelope volume envelope_volume284510 ų
Hydration-shell volume shell_volume60732 ų
Envelope diameter envelope_diameter139.6 Å
Shell Rg shell_rg45.23 Å
Envelope Rg envelope_rg37.96 Å
Shape Rg shape_rg38.21 Å
Total Rg total_rg38.56 Å
Total atoms total_atoms11273
Residues n_residues1448
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax130.4 Å
Rg (real space) rg_real38.76 Å
Rg uncertainty (real space) rg_real_error1.07 Å
I(0) (real space) i0_real3.8900e+08
I(0) uncertainty (real space) i0_real_error6.7090e+06
Rg (reciprocal space) rg_reciprocal38.86 Å
I(0) (reciprocal space) i0_reciprocal389000000.0000
Solution quality estimate total_estimate0.8872
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks2
Primary peak position r_peak_primary47.7 Å
Skewness Skewness skewness0.230
Kurtosis Kurtosis kurtosis-0.403
Angular range angular_range— – 0.2050 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha102000000.0000
Real-space data points n_real_points42
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.855; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.999; Smooth: 0.964
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (4)

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8. Citations (1)

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9. Files and Curves (10)