9e2e

The structure of the junction region of the wild-type murine native cardiac thin filament in Ca2+-free state

Method: ELECTRON MICROSCOPY D_max: 197.8 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	9e2e
沉积日期 deposition_date	2024-10-22
结构标题 title	The structure of the junction region of the wild-type murine native cardiac thin filament in Ca2+-free state
关键词 keywords	thin filament, troponin T, muscle, motor protein; MOTOR PROTEIN
实验方法 method	ELECTRON MICROSCOPY

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	56.03 Å
回转半径 Rg (电子) rg_electron	56.44 Å
零角强度 I(0) i0	1586770000.00
分子量 molecular_weight	327060.0 kDa
排除体积 excluded_volume	407280 Å³
包络体积 envelope_volume	601910 Å³
水化壳体积 shell_volume	92541 Å³
包络直径 envelope_diameter	213.7 Å
壳层 Rg shell_rg	54.97 Å
包络 Rg envelope_rg	56.22 Å
形状 Rg shape_rg	56.45 Å
总 Rg total_rg	56.35 Å
总原子数 total_atoms	22924
残基数 n_residues	2880
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	197.8 Å
Rg (实空间) rg_real	56.50 Å
Rg 误差 (实空间) rg_real_error	2.29 Å
I(0) (实空间) i0_real	1.5870e+09
I(0) 误差 (实空间) i0_real_error	3.0820e+07
Rg (倒空间) rg_reciprocal	55.64 Å
I(0) (倒空间) i0_reciprocal	1585000000.0000
解质量估计 total_estimate	0.8098
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	2
主峰位置 r_peak_primary	56.5 Å
偏度 Skewness skewness	0.622
峰度 Kurtosis kurtosis	-0.047
角度范围 angular_range	— – 0.1400 Å⁻¹
当前正则化参数 α current_alpha	0.0000
最高正则化参数 α highest_alpha	147600000.0000
实空间数据点数 n_real_points	29
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.665; Stabil: 0.999; Sysdev: 1.000; Positv: 1.000; Valcen: 0.952; Smooth: 0.579

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (5)

Entity 1 polymer

描述 description	Actin, alpha cardiac muscle 1
分子量 formula_weight	42064.9 kDa
EC 编号 ec_number	3.6.4.-
来源方法 src_method	nat
拷贝数 entity_count	6

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A,B,C,D,E,F
原子数 atom_count	22924

MCDDEETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDEAGPSIVHRKCF

Entity 2 polymer

描述 description	Tropomyosin alpha-1 chain
分子量 formula_weight	32735.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	8

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	G,H,I,J,L,M,N,O

MDAIKKKMQMLKLDKENALDRAEQAEADKKAAEDRSKQLEDELVSLQKKLKGTEDELDKYSEALKDAQEKLELAEKKATDAEADVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIESRAQKDEEKMEIQEIQLKEAKHIAEDADRKYEEVARKLVIIESDLERAEERAELSEGKCAELEEELKTVTNNLKSLEAQAEKYSQKEDKYEEEIKVLSDKLKEAETRAEFAERSVTKLEKSIDDLEDELYAQKLKYKAISEELDHALNDMTSI

Entity 3 polymer

描述 description	Isoform A2 of Troponin T, cardiac muscle
分子量 formula_weight	34616.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	2

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	K,P

MSDAEEVVEEYEEEQEEQEEAVEEEEAGGAEPEPEGEAETEEANVEEVGPDEEAKDAEEGPVEDTKPKPSRLFMPNLVPPKIPDGERVDFDDIHRKRVEKDLNELQTLIEAHFENRKKEEEELISLKDRIEKRRAERAEQQRIRNEREKERQNRLAEERARREEEENRRKAEDEARKKKALSNMMHFGGYIQKQAQTERKSGKRQTEREKKKKILAERRKALAIDHLNEDQLREKAKELWQSIHNLEAEKFDLQEKFKQQKYEINVLRNRINDNQKVSKTRGKAKVTGRWK

Entity 4 non-polymer

描述 description	ADENOSINE-5'-DIPHOSPHATE
分子量 formula_weight	427.2 kDa
来源方法 src_method	syn
拷贝数 entity_count	6

Entity 5 non-polymer

描述 description	MAGNESIUM ION
分子量 formula_weight	24.3 kDa
来源方法 src_method	syn
拷贝数 entity_count	6

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7. 引用文献 (1)

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分辨率 resolution	4.00 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	68660

fit	/app/data/saxs_computation/e2/9e2e/9e2e.fit
pddf	/app/data/pr_computation/e2/9e2e/9e2e.pr.out
profile	/app/data/saxs_computation/e2/9e2e/9e2e.dat

The structure of the junction region of the wild-type murine native cardiac thin filament in Ca2+-free state

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

冷冻电镜 EM 3D

5. 实体与聚合体信息 (5)

Entity 1 polymer

聚合体 Polymer

Entity 2 polymer

聚合体 Polymer

Entity 3 polymer

聚合体 Polymer

Entity 4 non-polymer

Entity 5 non-polymer

7. 引用文献 (1)

8. 文件与曲线 (10)

Files (7)

Curves (3)