9mrt

Cryo-EM structure of the human TRPM4 channel in a calcium and PI(4,5)P2 bound open state

Method: ELECTRON MICROSCOPY D_max: 155.7 Å Quality: GOOD

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Transient receptor potential cation channel subfamily M member 4

Homo sapiens

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein homooligomer Homooligomer Protein × 4 PDB declaration: tetrameric（4） Consistent with protein copy count	Chain A； UniProt 1–1214 Chain B； UniProt 1–1214 Chain C； UniProt 1–1214 Chain D； UniProt 1–1214	Not recorded	PT5 [(2R)-1-octadecanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phospho ryl]oxy-propan-2-yl] (8Z)-icosa-5,8,11,14-tetraenoate × 4 CA CALCIUM ION × 8	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE	Resolution 2.44 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 9MRT	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
30KW Human Trpm4 at 8 degrees Celsius Deposited 2026-05-01	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–1214（1214 aa） Chain B 1–1214（1214 aa） Chain C 1–1214（1214 aa） Chain D 1–1214（1214 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY mmCIF provides none of the parsed conditions	Resolution 3.10 Å
30KZ Human trpm4 in complex with PBA at 8 degrees Celsius Deposited 2026-05-01	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–1214（1214 aa） Chain B 1–1214（1214 aa） Chain C 1–1214（1214 aa） Chain D 1–1214（1214 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY mmCIF provides none of the parsed conditions	Resolution 3.40 Å
30LA Human Trpm4 with calcium at 37 degrees celsius Deposited 2026-05-01	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–1214（1214 aa） Chain B 1–1214（1214 aa） Chain C 1–1214（1214 aa） Chain D 1–1214（1214 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY mmCIF provides none of the parsed conditions	Resolution 3.00 Å
30LD Human Trpm4 with calcium and PBA at 37 degrees celsius Deposited 2026-05-01	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–1214（1214 aa） Chain B 1–1214（1214 aa） Chain C 1–1214（1214 aa） Chain D 1–1214（1214 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY mmCIF provides none of the parsed conditions	Resolution 3.00 Å
5WP6 Cryo-EM structure of a human TRPM4 channel in complex with calcium and decavanadate Deposited 2017-08-03	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–1214（1214 aa） Chain B 1–1214（1214 aa） Chain C 1–1214（1214 aa） Chain D 1–1214（1214 aa）	Not recorded	DVT DECAVANADATE × 8	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.80 Å
6BQR Human TRPM4 ion channel in lipid nanodiscs in a calcium-free state Deposited 2017-11-28	Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 75–1168（1094 aa） Chain B 75–1168（1094 aa） Chain C 75–1168（1094 aa） Chain D 75–1168（1094 aa）	Not recorded	Y01 CHOLESTEROL HEMISUCCINATE × 12	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
6BQV Human TRPM4 ion channel in lipid nanodiscs in a calcium-bound state Deposited 2017-11-28	Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 2–1214（1213 aa） Chain B 2–1214（1213 aa） Chain C 2–1214（1213 aa） Chain D 2–1214（1213 aa）	Not recorded	CA CALCIUM ION × 4 Y01 CHOLESTEROL HEMISUCCINATE × 12	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
8RCR human TRPM4 in SMA apo Deposited 2023-12-07	Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 421–1168（748 aa） Chain B 421–1168（748 aa） Chain C 421–1168（748 aa） Chain D 421–1168（748 aa）	Not recorded	CLR CHOLESTEROL × 12	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.60 Å
8RCU human TRPM4 in SMA IBA Deposited 2023-12-07	Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 421–1168（748 aa） Chain B 421–1168（748 aa） Chain C 421–1168（748 aa） Chain D 421–1168（748 aa）	Not recorded	A1HZ5 4-chloranyl-2-[2-(3-iodanylphenoxy)ethanoylamino]benzoic acid × 4 CLR CHOLESTEROL × 8	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.50 Å
8RD9 human TRPM4 in SMA NBA Deposited 2023-12-07	Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 420–1168（749 aa） Chain C 420–1168（749 aa） Chain D 420–1168（749 aa） Chain E 420–1168（749 aa）	Not recorded	A1H0C 4-chloranyl-2-(2-naphthalen-1-yloxyethanoylamino)benzoic acid × 4 CLR CHOLESTEROL × 8	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.30 Å
9B8W Cryo-EM structure of the human TRPM4 in complex with calcium at 37 degrees Celsius Deposited 2024-04-01	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–1214（1214 aa） Chain B 1–1214（1214 aa） Chain C 1–1214（1214 aa） Chain D 1–1214（1214 aa）	Not recorded	CA CALCIUM ION × 8	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
9B8X Cryo-EM structure of the human TRPM4 channel subunit in complex with calcium 37 degrees Celsius Deposited 2024-04-01	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–1214（1214 aa）	Not recorded	CA CALCIUM ION × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
9B8Y Cryo-EM structure of the human TRPM4 channel in complex with calcium and decavanadate at 37 degrees Celsius Deposited 2024-04-01	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–1214（1214 aa） Chain B 1–1214（1214 aa） Chain C 1–1214（1214 aa） Chain D 1–1214（1214 aa）	Not recorded	CA CALCIUM ION × 8 DVT DECAVANADATE × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
9B8Z Cryo-EM structure of the human TRPM4 channel subunit in complex with calcium and decavanadate at 37 degrees Celsius Deposited 2024-04-01	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–1214（1214 aa）	Not recorded	CA CALCIUM ION × 2 DVT DECAVANADATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.40 Å
9B90 Cryo-EM structure of the human TRPM4 channel in complex with calcium and ATP at 37 degrees Celsius Deposited 2024-04-01	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–1214（1214 aa） Chain B 1–1214（1214 aa） Chain C 1–1214（1214 aa） Chain D 1–1214（1214 aa）	Not recorded	CA CALCIUM ION × 8 ATP ADENOSINE-5'-TRIPHOSPHATE × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.40 Å
9B91 Cryo-EM structure of the human TRPM4 channel subunit in complex with calcium and ATP at 37 degrees Celsius Deposited 2024-04-01	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–1214（1214 aa）	Not recorded	CA CALCIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
9B92 Cryo-EM structure of the human TRPM4 in complex with calcium at 18 degrees Celsius Deposited 2024-04-01	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–1214（1214 aa） Chain B 1–1214（1214 aa） Chain C 1–1214（1214 aa） Chain D 1–1214（1214 aa）	Not recorded	CA CALCIUM ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.50 Å
9B93 Cryo-EM structure of the human TRPM4 channel in the presence of EDTA at 37 degrees Celsius Deposited 2024-04-01	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–1214（1214 aa） Chain B 1–1214（1214 aa） Chain C 1–1214（1214 aa） Chain D 1–1214（1214 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
9B94 Cryo-EM structure of the E396A mutant of human TRPM4 in complex with calcium at 37 degrees Celsius Deposited 2024-04-01	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–1214（1214 aa） Chain B 1–1214（1214 aa） Chain C 1–1214（1214 aa） Chain D 1–1214（1214 aa）	Mutation：E396A Mutation：E396A Mutation：E396A Mutation：E396A	CA CALCIUM ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
9I3R Human TRPM4 ion channel in MAASTY copolymer lipid nanodisc in a calcium-bound state Deposited 2025-01-23	Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 2–1214（1213 aa） Chain B 2–1214（1213 aa） Chain C 2–1214（1213 aa） Chain D 2–1214（1213 aa）	Not recorded	CLR CHOLESTEROL × 12 CA CALCIUM ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.46 Å
9MT8 Cryo-EM structure of the human TRPM4 channel in a calcium bound putative desensitized state Deposited 2025-01-10	Different ligand/ion Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–1214（1214 aa） Chain B 1–1214（1214 aa） Chain C 1–1214（1214 aa） Chain D 1–1214（1214 aa）	Not recorded	CA CALCIUM ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.19 Å
9MTA Cryo-EM structure of the human TRPM4 channel in an apo closed state Deposited 2025-01-10	Different ligand/ion Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–1214（1214 aa） Chain B 1–1214（1214 aa） Chain C 1–1214（1214 aa） Chain D 1–1214（1214 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.78 Å
9MTC Cryo-EM structure of the human TRPM4 channel in a ATP bound inhibited state Deposited 2025-01-10	Different ligand/ion Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–1214（1214 aa） Chain B 1–1214（1214 aa） Chain C 1–1214（1214 aa） Chain D 1–1214（1214 aa）	Not recorded	ATP ADENOSINE-5'-TRIPHOSPHATE × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.63 Å
9SMK Human TRPM4 ion channel in MSP2N2 lipid nanodisc in a calcium-bound state Deposited 2025-09-08	Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 2–1214（1213 aa） Chain B 2–1214（1213 aa） Chain C 2–1214（1213 aa） Chain D 2–1214（1213 aa）	Not recorded	CA CALCIUM ION × 4 Y01 CHOLESTEROL HEMISUCCINATE × 12	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.80 Å
9Y2A Cryo-EM structure of the human TRPM4 channel in complex with calcium and DAB at 37 degrees Celsius Deposited 2025-08-31	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–1214（1214 aa） Chain B 1–1214（1214 aa） Chain C 1–1214（1214 aa） Chain D 1–1214（1214 aa）	Not recorded	CA CALCIUM ION × 8 A1CR0 4,4'-[(pyridin-2-yl)methylene]diphenol × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
9Y2B Cryo-EM structure of the human TRPM4 channel in complex with EGTA and DAB at 37 degrees Celsius Deposited 2025-08-31	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–1214（1214 aa） Chain B 1–1214（1214 aa） Chain C 1–1214（1214 aa） Chain D 1–1214（1214 aa）	Not recorded	A1CR0 4,4'-[(pyridin-2-yl)methylene]diphenol × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
9Y2C Cryo-EM structure of the human TRPM4 channel subunit in complex with calcium and DAB at 37 degrees Celsius Deposited 2025-08-31	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–1214（1214 aa）	Not recorded	CA CALCIUM ION × 2 A1CR0 4,4'-[(pyridin-2-yl)methylene]diphenol × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.40 Å
9Z1W Cryo-EM structure of human TRPM4 channel in complex with calcium and TPPO at 37 degrees Celsius (consensus) Deposited 2025-11-04	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–1214（1214 aa） Chain B 1–1214（1214 aa） Chain C 1–1214（1214 aa） Chain D 1–1214（1214 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY mmCIF provides none of the parsed conditions	Resolution 2.86 Å
9Z1Z Cryo-EM structure of human TRPM4 channel in complex with calcium and TPPO at 18 degrees Celsius (consensus) Deposited 2025-11-04	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–1214（1214 aa） Chain B 1–1214（1214 aa） Chain C 1–1214（1214 aa） Chain D 1–1214（1214 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY mmCIF provides none of the parsed conditions	Resolution 2.63 Å
9Z20 Cryo-EM structure of human TRPM4 channel in complex with EGTA and TPPO at 37 degrees Celsius (consensus) Deposited 2025-11-04	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–1214（1214 aa） Chain B 1–1214（1214 aa） Chain C 1–1214（1214 aa） Chain D 1–1214（1214 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY mmCIF provides none of the parsed conditions	Resolution 2.51 Å
9Z21 Cryo-EM structure of human TRPM4 channel in complex with calcium and NC1 at 37 degrees Celsius (consensus) Deposited 2025-11-04	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–1214（1214 aa） Chain B 1–1214（1214 aa） Chain C 1–1214（1214 aa） Chain D 1–1214（1214 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY mmCIF provides none of the parsed conditions	Resolution 2.83 Å
9Z22 Cryo-EM structure of human TRPM4 channel in complex with EGTA and NC1 at 37 degrees Celsius (consensus) Deposited 2025-11-04	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–1214（1214 aa） Chain B 1–1214（1214 aa） Chain C 1–1214（1214 aa） Chain D 1–1214（1214 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY mmCIF provides none of the parsed conditions	Resolution 2.58 Å
9Z23 Cryo-EM structure of human TRPM4 channel in complex with calcium and CBA at 37 degrees Celsius (consensus) Deposited 2025-11-04	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–1214（1214 aa） Chain B 1–1214（1214 aa） Chain C 1–1214（1214 aa） Chain D 1–1214（1214 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY mmCIF provides none of the parsed conditions	Resolution 2.59 Å
9Z24 Cryo-EM structure of human TRPM4 channel in complex with calcium and CBA at 37 degrees Celsius (monomeric TMD-focused map with best-resolved CBA density) Deposited 2025-11-04	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–1214（1214 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY mmCIF provides none of the parsed conditions	Resolution 3.10 Å
9Z25 Cryo-EM structure of human TRPM4 channel in complex with calcium and NBA at 37 degrees Celsius (consensus) Deposited 2025-11-04	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–1214（1214 aa） Chain B 1–1214（1214 aa） Chain C 1–1214（1214 aa） Chain D 1–1214（1214 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY mmCIF provides none of the parsed conditions	Resolution 2.64 Å
9Z26 Cryo-EM structure of human TRPM4 channel in complex with calcium and NBA at 37 degrees Celsius (monomeric TMD-focused map with best-resolved NBA density) Deposited 2025-11-04	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–1214（1214 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY mmCIF provides none of the parsed conditions	Resolution 2.90 Å
9Z27 Cryo-EM structure of human TRPM4 channel in complex with calcium and CBA and DVT at 37 degrees Celsius (consensus) Deposited 2025-11-04	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–1214（1214 aa） Chain B 1–1214（1214 aa） Chain C 1–1214（1214 aa） Chain D 1–1214（1214 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY mmCIF provides none of the parsed conditions	Resolution 2.76 Å

37 other PDB entries and 37 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	TRPM4_HUMAN
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–1214； UniProt 1–1214 Author chain B； PDBConstruct 1–1214； UniProt 1–1214 Author chain C； PDBConstruct 1–1214； UniProt 1–1214 Author chain D； PDBConstruct 1–1214； UniProt 1–1214

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

P(r) Distance Distribution P(r) Distribution

Download Download SAXS (.dat) P(r) (.pr.out) CIF

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	9mrt
Deposition date deposition_date	2025-01-08
Structure title title	Cryo-EM structure of the human TRPM4 channel in a calcium and PI(4,5)P2 bound open state
Keywords keywords	TRPM4, Ion channel, TRANSPORT PROTEIN; TRANSPORT PROTEIN
Experimental Method method	ELECTRON MICROSCOPY

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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier	51.49 Å
Radius of gyration Rg (electron density) rg_electron	50.92 Å
Forward intensity I(0) i0	2501550000.00
Molecular weight molecular_weight	433700.0 kDa
Excluded volume excluded_volume	549230 Å³
Envelope volume envelope_volume	791260 Å³
Hydration-shell volume shell_volume	124100 Å³
Envelope diameter envelope_diameter	160.9 Å
Shell Rg shell_rg	59.28 Å
Envelope Rg envelope_rg	49.31 Å
Shape Rg shape_rg	50.95 Å
Total Rg total_rg	51.06 Å
Total atoms total_atoms	30544
Residues n_residues	3804
Spherical-harmonic order n_harmonics	20
q range q_range	— – 0.5000 Å⁻¹
Data points n_points	101
Shell type shell_type	directional
Solvent electron density solvent_density	0.3340 e/Å³
Shell contrast contrast_shell	0.0300 e/Å³
CRYSOL version crysol_version	4.1.3

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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax	155.7 Å
Rg (real space) rg_real	51.22 Å
Rg uncertainty (real space) rg_real_error	0.86 Å
I(0) (real space) i0_real	2.5020e+09
I(0) uncertainty (real space) i0_real_error	4.4310e+07
Rg (reciprocal space) rg_reciprocal	51.71 Å
I(0) (reciprocal space) i0_reciprocal	2503000000.0000
Solution quality estimate total_estimate	0.8728
Solution quality rating solution_quality	GOOD a GOOD solution
P(r) peaks n_peaks	1
Primary peak position r_peak_primary	70.3 Å
Skewness Skewness skewness	0.081
Kurtosis Kurtosis kurtosis	-0.473
Angular range angular_range	— – 0.1550 Å⁻¹
Current regularization parameter α current_alpha	0.0000
Highest regularization parameter α highest_alpha	288100000.0000
Real-space data points n_real_points	32
GNOM version gnom_version	4.1.3
Quality Criteria quality_criteria	AN1: 0.000; Oscil: 0.927; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.951; Smooth: 0.612

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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (3)

Entity 1 Entity 1 polymer

Description description	Transient receptor potential cation channel subfamily M member 4
Molecular weight formula_weight	134456.5 kDa
Source method src_method	man
Copy count entity_count	4

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	B,A,C,D
Atom count atom_count	30544

MVVPEKEQSWIPKIFKKKTCTTFIVDSTDPGGTLCQCGRPRTAHPAVAMEDAFGAAVVTVWDSDAHTTEKPTDAYGELDFTGAGRKHSNFLRLSDRTDPAAVYSLVTRTWGFRAPNLVVSVLGGSGGPVLQTWLQDLLRRGLVRAAQSTGAWIVTGGLHTGIGRHVGVAVRDHQMASTGGTKVVAMGVAPWGVVRNRDTLINPKGSFPARYRWRGDPEDGVQFPLDYNYSAFFLVDDGTHGCLGGENRFRLRLESYISQQKTGVGGTGIDIPVLLLLIDGDEKMLTRIENATQAQLPCLLVAGSGGAADCLAETLEDTLAPGSGGARQGEARDRIRRFFPKGDLEVLQAQVERIMTRKELLTVYSSEDGSEEFETIVLKALVKACGSSEASAYLDELRLAVAWNRVDIAQSELFRGDIQWRSFHLEASLMDALLNDRPEFVRLLISHGLSLGHFLTPMRLAQLYSAAPSNSLIRNLLDQASHSAGTKAPALKGGAAELRPPDVGHVLRMLLGKMCAPRYPSGGAWDPHPGQGFGESMYLLSDKATSPLSLDAGLGQAPWSDLLLWALLLNRAQMAMYFWEMGSNAVSSALGACLLLRVMARLEPDAEEAARRKDLAFKFEGMGVDLFGECYRSSEVRAARLLLRRCPLWGDATCLQLAMQADARAFFAQDGVQSLLTQKWWGDMASTTPIWALVLAFFCPPLIYTRLITFRKSEEEPTREELEFDMDSVINGEGPVGTADPAEKTPLGVPRQSGRPGCCGGRCGGRRCLRRWFHFWGAPVTIFMGNVVSYLLFLLLFSRVLLVDFQPAPPGSLELLLYFWAFTLLCEELRQGLSGGGGSLASGGPGPGHASLSQRLRLYLADSWNQCDLVALTCFLLGVGCRLTPGLYHLGRTVLCIDFMVFTVRLLHIFTVNKQLGPKIVIVSKMMKDVFFFLFFLGVWLVAYGVATEGLLRPRDSDFPSILRRVFYRPYLQIFGQIPQEDMDVALMEHSNCSSEPGFWAHPPGAQAGTCVSQYANWLVVLLLVIFLLVANILLVNLLIAMFSYTFGKVQGNSDLYWKAQRYRLIREFHSRPALAPPFIVISHLRLLLRQLCRRPRSPQPSSPALEHFRVYLSKEAERKLLTWESVHKENFLLARARDKRESDSERLKRTSQKVDLALKQLGHIREYEQRLKVLEREVQQCSRVLGWVAEALSRSALLPPGGPPPPDLPGSKD

来源 Source

来源生物 organism	Homo sapiens
通用名 common_name	human
NCBI Class编号 ncbi_taxonomy_id	9606
宿主生物 host_organism	Homo sapiens

Entity 2 Entity 2 non-polymer

Description description	;[(2R)-1-octadecanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phospho ryl]oxy-propan-2-yl] (8Z)-icosa-5,8,11,14-tetraenoate ;
Molecular weight formula_weight	1047.1 kDa
Source method src_method	syn
Copy count entity_count	4

Entity 3 Entity 3 non-polymer

Description description	CALCIUM ION
Molecular weight formula_weight	40.1 kDa
Source method src_method	syn
Copy count entity_count	8

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8. Citations (1)

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Unit-cell length a length_a	1.000 Å
Unit-cell length b length_b	1.000 Å
Unit-cell length c length_c	1.000 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	—

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	triclinic

Resolution resolution	2.44 Å
Resolution测定方法 resolution_method	—
颗粒数 num_particles	32765

fit	/app/data/saxs_computation/mr/9mrt/9mrt.fit
pddf	/app/data/pr_computation/mr/9mrt/9mrt.pr.out
profile	/app/data/saxs_computation/mr/9mrt/9mrt.dat