9ntm

SPEF1 bound to 14-pf microtubule

Method: ELECTRON MICROSCOPY

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Sperm flagellar protein 1,G protein/GFP fusion protein

Recombinant vesicular stomatitis Indiana virus rVSV-G/GFP

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Insufficient information	Heteromer	Protein 89	Tubulin alpha-1B chain × 42 （P81947） Tubulin beta chain × 42 （A0A4W2DT89） GUANOSINE-5'-TRIPHOSPHATE × 42 MAGNESIUM ION × 42 GUANOSINE-5'-DIPHOSPHATE × 42 TAXOL × 42	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	B7UCZ6_9RHAB
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain 1A； PDBConstruct 141–379； UniProt 512–750 Author chain 1B； PDBConstruct 141–379； UniProt 512–750 Author chain 1C； PDBConstruct 141–379； UniProt 512–750 Author chain 1D； PDBConstruct 141–379； UniProt 512–750 Author chain 1E； PDBConstruct 141–379； UniProt 512–750

Sperm flagellar protein 1,G protein/GFP fusion protein

Recombinant vesicular stomatitis Indiana virus rVSV-G/GFP

UniProt Q9Y4P9

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Insufficient information	Heteromer	Protein 89	Tubulin alpha-1B chain × 42 （P81947） Tubulin beta chain × 42 （A0A4W2DT89） GUANOSINE-5'-TRIPHOSPHATE × 42 MAGNESIUM ION × 42 GUANOSINE-5'-DIPHOSPHATE × 42 TAXOL × 42	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	SPEF1_HUMAN
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain 1A； PDBConstruct 11–135； UniProt 1–125 Author chain 1B； PDBConstruct 11–135； UniProt 1–125 Author chain 1C； PDBConstruct 11–135； UniProt 1–125 Author chain 1D； PDBConstruct 11–135； UniProt 1–125 Author chain 1E； PDBConstruct 11–135； UniProt 1–125

Tubulin alpha-1B chain

OrganismNot specified

UniProt P81947

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Insufficient information	Heteromer	Protein 89	Sperm flagellar protein 1,G protein/GFP fusion protein × 5 （B7UCZ6,Q9Y4P9） Tubulin beta chain × 42 （A0A4W2DT89） GUANOSINE-5'-TRIPHOSPHATE × 42 MAGNESIUM ION × 42 GUANOSINE-5'-DIPHOSPHATE × 42 TAXOL × 42	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	TBA1B_BOVIN
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain AA； PDBConstruct 1–451； UniProt 1–451 Author chain AC； PDBConstruct 1–451； UniProt 1–451 Author chain AE； PDBConstruct 1–451； UniProt 1–451 Author chain BA； PDBConstruct 1–451； UniProt 1–451 Author chain BC； PDBConstruct 1–451； UniProt 1–451 Author chain BE； PDBConstruct 1–451； UniProt 1–451 Author chain CA； PDBConstruct 1–451； UniProt 1–451 Author chain CC； PDBConstruct 1–451； UniProt 1–451 Author chain CE； PDBConstruct 1–451； UniProt 1–451 Author chain DA； PDBConstruct 1–451； UniProt 1–451 Author chain DC； PDBConstruct 1–451； UniProt 1–451 Author chain DE； PDBConstruct 1–451； UniProt 1–451 Author chain EA； PDBConstruct 1–451； UniProt 1–451 Author chain EC； PDBConstruct 1–451； UniProt 1–451 Author chain EE； PDBConstruct 1–451； UniProt 1–451 Author chain FA； PDBConstruct 1–451； UniProt 1–451 Author chain FC； PDBConstruct 1–451； UniProt 1–451 Author chain FE； PDBConstruct 1–451； UniProt 1–451 Author chain GA； PDBConstruct 1–451； UniProt 1–451 Author chain GC； PDBConstruct 1–451； UniProt 1–451 Author chain GE； PDBConstruct 1–451； UniProt 1–451 Author chain HA； PDBConstruct 1–451； UniProt 1–451 Author chain HC； PDBConstruct 1–451； UniProt 1–451 Author chain HE； PDBConstruct 1–451； UniProt 1–451 Author chain IA； PDBConstruct 1–451； UniProt 1–451 Author chain IC； PDBConstruct 1–451； UniProt 1–451 Author chain IE； PDBConstruct 1–451； UniProt 1–451 Author chain JA； PDBConstruct 1–451； UniProt 1–451 Author chain JC； PDBConstruct 1–451； UniProt 1–451 Author chain JE； PDBConstruct 1–451； UniProt 1–451 Author chain KA； PDBConstruct 1–451； UniProt 1–451 Author chain KC； PDBConstruct 1–451； UniProt 1–451 Author chain KE； PDBConstruct 1–451； UniProt 1–451 Author chain LA； PDBConstruct 1–451； UniProt 1–451 Author chain LC； PDBConstruct 1–451； UniProt 1–451 Author chain LE； PDBConstruct 1–451； UniProt 1–451 Author chain MA； PDBConstruct 1–451； UniProt 1–451 Author chain MC； PDBConstruct 1–451； UniProt 1–451 Author chain ME； PDBConstruct 1–451； UniProt 1–451 Author chain NA； PDBConstruct 1–451； UniProt 1–451 Author chain NC； PDBConstruct 1–451； UniProt 1–451 Author chain NE； PDBConstruct 1–451； UniProt 1–451

Tubulin beta chain

OrganismNot specified

UniProt A0A4W2DT89

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Insufficient information	Heteromer	Protein 89	Sperm flagellar protein 1,G protein/GFP fusion protein × 5 （B7UCZ6,Q9Y4P9） Tubulin alpha-1B chain × 42 （P81947） GUANOSINE-5'-TRIPHOSPHATE × 42 MAGNESIUM ION × 42 GUANOSINE-5'-DIPHOSPHATE × 42 TAXOL × 42	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A4W2DT89_BOBOX
Isoform	—
PDB entities	3
Chains and sequence ranges	Author chain AB； PDBConstruct 1–445； UniProt 1–445 Author chain AD； PDBConstruct 1–445； UniProt 1–445 Author chain AF； PDBConstruct 1–445； UniProt 1–445 Author chain BB； PDBConstruct 1–445； UniProt 1–445 Author chain BD； PDBConstruct 1–445； UniProt 1–445 Author chain BF； PDBConstruct 1–445； UniProt 1–445 Author chain CB； PDBConstruct 1–445； UniProt 1–445 Author chain CD； PDBConstruct 1–445； UniProt 1–445 Author chain CF； PDBConstruct 1–445； UniProt 1–445 Author chain DB； PDBConstruct 1–445； UniProt 1–445 Author chain DD； PDBConstruct 1–445； UniProt 1–445 Author chain DF； PDBConstruct 1–445； UniProt 1–445 Author chain EB； PDBConstruct 1–445； UniProt 1–445 Author chain ED； PDBConstruct 1–445； UniProt 1–445 Author chain EF； PDBConstruct 1–445； UniProt 1–445 Author chain FB； PDBConstruct 1–445； UniProt 1–445 Author chain FD； PDBConstruct 1–445； UniProt 1–445 Author chain FF； PDBConstruct 1–445； UniProt 1–445 Author chain GB； PDBConstruct 1–445； UniProt 1–445 Author chain GD； PDBConstruct 1–445； UniProt 1–445 Author chain GF； PDBConstruct 1–445； UniProt 1–445 Author chain HB； PDBConstruct 1–445； UniProt 1–445 Author chain HD； PDBConstruct 1–445； UniProt 1–445 Author chain HF； PDBConstruct 1–445； UniProt 1–445 Author chain IB； PDBConstruct 1–445； UniProt 1–445 Author chain ID； PDBConstruct 1–445； UniProt 1–445 Author chain IF； PDBConstruct 1–445； UniProt 1–445 Author chain JB； PDBConstruct 1–445； UniProt 1–445 Author chain JD； PDBConstruct 1–445； UniProt 1–445 Author chain JF； PDBConstruct 1–445； UniProt 1–445 Author chain KB； PDBConstruct 1–445； UniProt 1–445 Author chain KD； PDBConstruct 1–445； UniProt 1–445 Author chain KF； PDBConstruct 1–445； UniProt 1–445 Author chain LB； PDBConstruct 1–445； UniProt 1–445 Author chain LD； PDBConstruct 1–445； UniProt 1–445 Author chain LF； PDBConstruct 1–445； UniProt 1–445 Author chain MB； PDBConstruct 1–445； UniProt 1–445 Author chain MD； PDBConstruct 1–445； UniProt 1–445 Author chain MF； PDBConstruct 1–445； UniProt 1–445 Author chain NB； PDBConstruct 1–445； UniProt 1–445 Author chain ND； PDBConstruct 1–445； UniProt 1–445 Author chain NF； PDBConstruct 1–445； UniProt 1–445

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	9ntm
Deposition date deposition_date	2025-03-18
Structure title title	SPEF1 bound to 14-pf microtubule
Keywords keywords	microtubules, spef1, seam, PROTEIN BINDING; PROTEIN BINDING
Experimental Method method	ELECTRON MICROSCOPY

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

9ntm__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

9ntm__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

9ntm__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	0.00 Å
Rg (electron density)	146.00 Å
Total Rg	146.00 Å
Atom count	292618
Residues	36552
Excluded volume	5169700 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`9ntm__assembly_1__model_1`	89-meric （89）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (7)

Entity 1 Entity 1 polymer

Description description	Sperm flagellar protein 1,G protein/GFP fusion protein
Molecular weight formula_weight	44324.3 kDa
Source method src_method	man
Copy count entity_count	5

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	1A,1B,1C,1D,1E
Atom count atom_count	292618

PTMGSSGSSGMASSVDEEALHQLYLWVDNIPLSRPKRNLSRDFSDGVLVAEVIKFYFPKMVEMHNYVPANSLQQKLSNWGHLNRKVLKRLNFSVPDDVMRKIAQCAPGVVELVLIPLRQRLEERQRRRKQGAGSLSSPQQMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGSAAAAWSHPQFEK

Source Source

Source organism organism	Recombinant vesicular stomatitis Indiana virus rVSV-G/GFP
NCBI taxonomy ID ncbi_taxonomy_id	582817
Host organism host_organism	Escherichia coli BL21(DE3)

Entity 2 Entity 2 polymer

Description description	Tubulin alpha-1B chain
Molecular weight formula_weight	50204.4 kDa
Source method src_method	nat
Copy count entity_count	42

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	AA,AC,AE,BA,BC,BE,CA,CC,CE,DA,DC,DE,EA,EC,EE,FA,FC,FE,GA,GC,GE,HA,HC,HE,IA,IC,IE,JA,JC,JE,KA,KC,KE,LA,LC,LE,MA,MC,ME,NA,NC,NE

MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGVDSVEGEGEEEGEEY

Entity 3 Entity 3 polymer

Description description	Tubulin beta chain
Molecular weight formula_weight	49983.8 kDa
Source method src_method	nat
Copy count entity_count	42

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	AB,AD,AF,BB,BD,BF,CB,CD,CF,DB,DD,DF,EB,ED,EF,FB,FD,FF,GB,GD,GF,HB,HD,HF,IB,ID,IF,JB,JD,JF,KB,KD,KF,LB,LD,LF,MB,MD,MF,NB,ND,NF

MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGNKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETYSIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATADEQGEFEEEEGEDEA

Entity 4 Entity 4 non-polymer

Description description	GUANOSINE-5'-TRIPHOSPHATE
Molecular weight formula_weight	523.2 kDa
Source method src_method	syn
Copy count entity_count	42

Entity 5 Entity 5 non-polymer

Description description	MAGNESIUM ION
Molecular weight formula_weight	24.3 kDa
Source method src_method	syn
Copy count entity_count	42

Entity 6 Entity 6 non-polymer

Description description	GUANOSINE-5'-DIPHOSPHATE
Molecular weight formula_weight	443.2 kDa
Source method src_method	syn
Copy count entity_count	42

Entity 7 Entity 7 non-polymer

Description description	TAXOL
Molecular weight formula_weight	853.9 kDa
Source method src_method	syn
Copy count entity_count	42

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Unit-cell length a length_a	1.000 Å
Unit-cell length b length_b	1.000 Å
Unit-cell length c length_c	1.000 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	—

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	triclinic

Resolution resolution	7.10 Å
Resolution测定Method resolution_method	—
颗粒数 num_particles	9625

SPEF1 bound to 14-pf microtubule

1. Protein Identity and Related Structures Protein Identity & Related Structures

Sperm flagellar protein 1,G protein/GFP fusion protein

State in the Current Structure

Other States of the Same Protein in the Database

Sperm flagellar protein 1,G protein/GFP fusion protein

State in the Current Structure

Other States of the Same Protein in the Database

Tubulin alpha-1B chain

State in the Current Structure

Other States of the Same Protein in the Database

Tubulin beta chain

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

9ntm__assembly_1__model_1

9ntm__assembly_1__model_1 | I(q)

9ntm__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

冷冻电镜 EM 3D

5. Entities and Polymers Entities & Polymers (7)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 polymer

Polymer Polymer

Entity 3 Entity 3 polymer

Polymer Polymer

Entity 4 Entity 4 non-polymer

Entity 5 Entity 5 non-polymer

Entity 6 Entity 6 non-polymer

Entity 7 Entity 7 non-polymer

7. Citations (1)

SPEF1 bound to 14-pf microtubule

1. Protein Identity and Related Structures Protein Identity & Related Structures

Sperm flagellar protein 1,G protein/GFP fusion protein

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Sperm flagellar protein 1,G protein/GFP fusion protein

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Tubulin alpha-1B chain

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Tubulin beta chain

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

9ntm__assembly_1__model_1

9ntm__assembly_1__model_1 | I(q)

9ntm__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

冷冻电镜 EM 3D

5. Entities and Polymers Entities & Polymers (7)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 polymer

Polymer Polymer

Entity 3 Entity 3 polymer

Polymer Polymer

Entity 4 Entity 4 non-polymer

Entity 5 Entity 5 non-polymer

Entity 6 Entity 6 non-polymer

Entity 7 Entity 7 non-polymer

7. Citations (1)