9oqq

Human pannexin 1 channel W74A mutant with 10mM ATP

Method: ELECTRON MICROSCOPY D_max: 128.1 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

▼

1. 结构基本信息 1. Structure Basics

条目编号 entry_id	9oqq
沉积日期 deposition_date	2025-05-21
结构标题 title	Human pannexin 1 channel W74A mutant with 10mM ATP
关键词 keywords	PANX1, Pannexin, Large pore channel, TRANSPORT PROTEIN; TRANSPORT PROTEIN
实验方法 method	ELECTRON MICROSCOPY

▼

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	42.35 Å
回转半径 Rg (电子) rg_electron	40.88 Å
零角强度 I(0) i0	789332000.00
分子量 molecular_weight	257590.0 kDa
排除体积 excluded_volume	332950 Å³
包络体积 envelope_volume	438250 Å³
水化壳体积 shell_volume	84644 Å³
包络直径 envelope_diameter	126.7 Å
壳层 Rg shell_rg	50.25 Å
包络 Rg envelope_rg	39.75 Å
形状 Rg shape_rg	40.90 Å
总 Rg total_rg	41.29 Å
总原子数 total_atoms	18221
残基数 n_residues	2408
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

▼

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	128.1 Å
Rg (实空间) rg_real	42.04 Å
Rg 误差 (实空间) rg_real_error	0.73 Å
I(0) (实空间) i0_real	7.8930e+08
I(0) 误差 (实空间) i0_real_error	1.2740e+07
Rg (倒空间) rg_reciprocal	42.35 Å
I(0) (倒空间) i0_reciprocal	789600000.0000
解质量估计 total_estimate	0.8237
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	1
主峰位置 r_peak_primary	58.8 Å
偏度 Skewness skewness	-0.026
峰度 Kurtosis kurtosis	-0.569
角度范围 angular_range	— – 0.1850 Å⁻¹
当前正则化参数 α current_alpha	0.0000
最高正则化参数 α highest_alpha	127900000.0000
实空间数据点数 n_real_points	38
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.915; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.959; Smooth: 0.000

▶

4. 晶体学与实验 4. Crystallography & Experiment

▶

5. 实体与聚合体信息 (2)

Entity 1 polymer

描述 description	Pannexin-1,RNA-directed RNA polymerase L chimera
分子量 formula_weight	77886.4 kDa
EC 编号 ec_number	2.1.1.375,2.7.7.48,2.7.7.88
突变 mutation	W74A
来源方法 src_method	man
拷贝数 entity_count	7

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A,B,C,D,E,F,G
原子数 atom_count	18221

MAIAQLATEYVFSDFLLKEPTEPKFKGLRLELAVDKMVTCIAVGLPLLLISLAFAQEISIGTQISCFSPSSFSARQAAFVDSYCWAAVQQKNSLQSESGNLPLWLHKFFPYILLLFAILLYLPPLFWRFAAAPHICSDLKFIMEELDKVYNRAIKAAKSARDLDMRDGACSVPGVTENLGQSLWEVSESHFKYPIVEQYLKTKKNSNNLIIKYISCRLLTLIIILLACIYLGYYFSLSSLSDEFVCSIKSGILRNDSTVPDQFQCKLIAVGIFQLLSVINLVVYVLLAPVVVYTLFVPFRQKTDVLKVYEILPTFDVLHFKSEGYNDLSLYNLFLEENISEVKSYKCLKVLENIKSSGQGIDPMLLLTNLGMIKMDVVDGKTPMSAEMREEQGNQTAELQGMNIDSETKANNGEKNARQRLLDSSCFESRLVPRGSAAAAVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSHHHHHHHH

来源 Source

来源生物 organism	Human respiratory syncytial virus
NCBI 分类编号 ncbi_taxonomy_id	11250
宿主生物 host_organism	Homo sapiens

Entity 2 non-polymer

描述 description	2-acetamido-2-deoxy-beta-D-glucopyranose
分子量 formula_weight	221.2 kDa
来源方法 src_method	syn
拷贝数 entity_count	7

▶

7. 引用文献 (1)

▶

分辨率 resolution	3.25 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	584553

fit	/app/data/saxs_computation/oq/9oqq/9oqq.fit
pddf	/app/data/pr_computation/oq/9oqq/9oqq.pr.out
profile	/app/data/saxs_computation/oq/9oqq/9oqq.dat