9pis

Ab initio structure of crambin by MicroED at 0.85A

Method: ELECTRON CRYSTALLOGRAPHY D_max: 35.8 Å Quality: GOOD

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Crambin

OrganismNot specified

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count	Chain A； UniProt 1–46	Not recorded	No other associated polymer	ELECTRON CRYSTALLOGRAPHY cryo-EM buffer：pH 7；Simple mixture of ethanol and water. cryo-EM vitrification conditions：Cryogen ETHANE	Resolution 0.85 Å R-free 0.164

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 9PIS	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
1AB1 SI FORM CRAMBIN Deposited 1997-01-31	Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–46（46 aa）	Not recorded	EOH ETHANOL × 1	X-RAY DIFFRACTION X-ray crystallization conditions vapor diffusion - hanging drop - microseeding；pH 7；HPLC PURIFIED SI-CRAMBIN PROTEIN WAS DISSOLVED AT 25 MG/ML IN 80% ETHANOL/WATER (V/V), AND EQUILIBRATED AGAINS 60% ETHANOL. AFTER 10 DAYS, THE RESERVOIR WAS LOWERED TO 50% AND MICROSEEDED BY THE STREAK SEEDING METHOD (CAT WHISKER). AFTER LOWERING THE RESERVOIR TO 45%, GOOD CRYSTALS WERE FORMED., pH 7., vapor diffusion - hanging drop - microseeding	Resolution 0.89 Å
1CBN ATOMIC RESOLUTION (0.83 ANGSTROMS) CRYSTAL STRUCTURE OF THE HYDROPHOBIC PROTEIN CRAMBIN AT 130 K Deposited 1991-10-11	Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–46（46 aa）	Not recorded	EOH ETHANOL × 1	X-RAY DIFFRACTION mmCIF provides none of the parsed conditions	Resolution 0.83 Å
1CCM DIRECT NOE REFINEMENT OF CRAMBIN FROM 2D NMR DATA USING A SLOW-COOLING ANNEALING PROTOCOL Deposited 1993-04-14	Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–46（46 aa）	Not recorded	No recorded non-water small molecule	SOLUTION NMR mmCIF provides none of the parsed conditions	Resolution not provided
1CCN DIRECT NOE REFINEMENT OF CRAMBIN FROM 2D NMR DATA USING A SLOW-COOLING ANNEALING PROTOCOL Deposited 1993-04-14	Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–46（46 aa）	Not recorded	No recorded non-water small molecule	SOLUTION NMR mmCIF provides none of the parsed conditions	Resolution not provided
1CNR CORRELATED DISORDER OF THE PURE PRO22(SLASH)LEU25 FORM OF CRAMBIN AT 150K REFINED TO 1.05 ANGSTROMS RESOLUTION Deposited 1993-07-15	Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–46（46 aa）	Not recorded	EOH ETHANOL × 1	X-RAY DIFFRACTION mmCIF provides none of the parsed conditions	Resolution 1.05 Å
1CRN WATER STRUCTURE OF A HYDROPHOBIC PROTEIN AT ATOMIC RESOLUTION. PENTAGON RINGS OF WATER MOLECULES IN CRYSTALS OF CRAMBIN Deposited 1981-04-30	Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–46（46 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION mmCIF provides none of the parsed conditions	Resolution 1.50 Å
1CXR AUTOMATED 2D NOESY ASSIGNMENT AND STRUCTURE CALCULATION OF CRAMBIN(S22/I25) WITH SELF-CORRECTING DISTANCE GEOMETRY BASED NOAH/DIAMOD PROGRAMS Deposited 1999-08-30	Different construct Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–46（46 aa） Fragment：ISOFORM OF CRAMBIN (46 RESIDUES)	Mutation：S22P, I25L	No recorded non-water small molecule	SOLUTION NMR NMR measurement conditions pH 6.5；298 K；Pressure 1 NMR sample composition 2.5 MM CRAMBIN(SER/ILE) 0.7 ML OF 75% D6-ACETONE, 20%H2O,5%D2O BUFFER	Resolution not provided
1EJG CRAMBIN AT ULTRA-HIGH RESOLUTION: VALENCE ELECTRON DENSITY. Deposited 2000-03-02	Different construct Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–46（46 aa） Fragment：CRAMBIN	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；The protein (40 mg/ml in 80% ethanol) is equilibrated against 60% ethanol. Not buffered., pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 293K	Resolution 0.54 Å R-free 0.094
1JXT CRAMBIN MIXED SEQUENCE FORM AT 160 K. PROTEIN/WATER SUBSTATES Deposited 2001-09-08	Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–46（46 aa）	Not recorded	EOH ETHANOL × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；ethanol, pH 7.00, VAPOR DIFFUSION, SITTING DROP, temperature 293K	Resolution 0.89 Å
1JXU CRAMBIN MIXED SEQUENCE FORM AT 240 K. PROTEIN/WATER SUBSTATES Deposited 2001-09-09	Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–46（46 aa）	Not recorded	EOH ETHANOL × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；ethanol, pH 7.00, VAPOR DIFFUSION, SITTING DROP, temperature 293K	Resolution 0.99 Å
1JXW CRAMBIN MIXED SEQUENCE FORM AT 180 K. PROTEIN/WATER SUBSTATES Deposited 2001-09-10	Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–46（46 aa）	Not recorded	EOH ETHANOL × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；ethanol, pH 7.00, VAPOR DIFFUSION, SITTING DROP, temperature 293K	Resolution 0.89 Å
1JXX CRAMBIN MIXED SEQUENCE FORM AT 200 K. PROTEIN/WATER SUBSTATES Deposited 2001-09-10	Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–46（46 aa）	Not recorded	EOH ETHANOL × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；ethanol, pH 7.00, VAPOR DIFFUSION, SITTING DROP, temperature 293K	Resolution 0.89 Å
1JXY CRAMBIN MIXED SEQUENCE FORM AT 220 K. PROTEIN/WATER SUBSTATES Deposited 2001-09-10	Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–46（46 aa）	Not recorded	EOH ETHANOL × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；ethanol, pH 7.00, VAPOR DIFFUSION, SITTING DROP, temperature 293K	Resolution 0.89 Å
1YV8 Solution structure of crambin in acetone/water mixed solvent Deposited 2005-02-15	Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–46（46 aa）	Not recorded	No recorded non-water small molecule	SOLUTION NMR NMR measurement conditions 298 K；Ionic strength (raw mmCIF value) 0；Pressure ambient NMR sample composition 1mM crambin U-15N,13C, 75% acetone, 15% H2O, 10% D2O \| 75% acetone, 15% H2O, 10% D2O	Resolution not provided
1YVA NMR solution structure of crambin in DPC micelles Deposited 2005-02-15	Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–46（46 aa）	Not recorded	No recorded non-water small molecule	SOLUTION NMR NMR measurement conditions pH 6；298 K；Ionic strength (raw mmCIF value) 0；Pressure ambient NMR sample composition 0.5 mM crambin U-15N,13C, pH 6.0, 20 mM phosphate buffer, 300mM DPC, 90% H2O, 10% D2O \| 300 mM DPC, 20 mM phosphate buffer, 90% H2O, 10% D2O	Resolution not provided
2EYA DMSO refined solution structure of crambin in acetone/water Deposited 2005-11-09	Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–46（46 aa）	Not recorded	No recorded non-water small molecule	SOLUTION NMR NMR measurement conditions 298 K；Pressure AMBIENT NMR sample composition 1MM CRAMBIN U-15N,13C, 75% ACETONE, 15% H2O, 10% D2O \| 75% ACETONE, 15% H2O, 10% D2O	Resolution not provided
2EYB Water refined solution structure of crambin in ACETONE/WATER Deposited 2005-11-09	Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–46（46 aa）	Not recorded	No recorded non-water small molecule	SOLUTION NMR NMR measurement conditions 298 K；Ionic strength (raw mmCIF value) 0；Pressure AMBIENT NMR sample composition 1MM CRAMBIN U-15N,13C, 75% ACETONE, 15% H2O, 10% D2O \| 75% ACETONE, 15% H2O, 10% D2O	Resolution not provided
2EYC DMSO refined solution structure of crambin in dpc micelles Deposited 2005-11-09	Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–46（46 aa）	Not recorded	No recorded non-water small molecule	SOLUTION NMR NMR measurement conditions pH 6；298 K；Ionic strength (raw mmCIF value) 0；Pressure AMBIENT NMR sample composition 0.5 MM CRAMBIN U-15N,13C, PH 6.0, 20 MM PHOSPHATE BUFFER, 300MM DPC, 90% H2O, 10% D2O \| 20 MM PHOSPHATE BUFFER, 300MM DPC, 90% H2O, 10% D2O	Resolution not provided
2EYD Water refined solution structure of crambin in dpc micelles Deposited 2005-11-09	Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–46（46 aa）	Not recorded	No recorded non-water small molecule	SOLUTION NMR NMR measurement conditions pH 6；298 K；Ionic strength (raw mmCIF value) 0；Pressure AMBIENT NMR sample composition 0.5 MM CRAMBIN U-15N,13C, PH 6.0, 20 MM PHOSPHATE BUFFER, 300MM DPC, 90% H2O, 10% D2O \| 20 MM PHOSPHATE BUFFER, 300MM DPC, 90% H2O, 10% D2O	Resolution not provided
2FD7 X-ray Crystal Structure of Chemically Synthesized Crambin Deposited 2005-12-13	Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–46（46 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；The native crambin crystals were grown from the aqueous solution by mixing a 2 ul aliquot of a crambin (10 mg/ml in pH 8.0, 100mM HEPES buffer containing 150mM NaCl) and 2 ul of a 0.8 M succinic acid, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 1.75 Å R-free 0.185
2FD9 X-ray Crystal Structure of Chemically Synthesized Crambin-{alpha}carboxamide Deposited 2005-12-13	Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–46（46 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；X-ray crystallography-quality crystals for crambin-carboxamide were formed by mixing 2 ul of protein (10 mg/ml in pH 7.5 100mM HEPES buffer containing 150 mM NaCl) and 2 ul of a 0.8 M succinic acid (used for X-ray diffraction), or by mixing 2 ul of the protein solution with 2 ul of 0.1 M HEPES buffer containing 15% v/v tacsimate and 2% w/v polyethylene glycol 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K	Resolution 1.60 Å R-free 0.234
3NIR Crystal structure of small protein crambin at 0.48 A resolution Deposited 2010-06-16	Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–46（46 aa）	Not recorded	EOH ETHANOL × 4	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；60% ethanol in water, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 293K	Resolution 0.48 Å
3U7T Room temperature ultra-high resolution time-of-flight neutron and X-ray diffraction studies of H/D exchanged crambin Deposited 2011-10-14	Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–46（46 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Vapor diffusion of protein in 80 % EtOH solution against a 55 % EtOH solution, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K	Resolution 0.85 Å R-free 0.136
3UE7 X-ray crystal structure of a novel topological analogue of crambin Deposited 2011-10-28	Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain B 1–46（46 aa） Fragment：L-crambin topological analogue	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.7；292 K；100 mM imidazole, 200 mM Zinc chloride, 7.5% Ethanol, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 292K	Resolution 1.08 Å R-free 0.176
3UE7 X-ray crystal structure of a novel topological analogue of crambin Deposited 2011-10-28	Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain B 1–46（46 aa） Fragment：L-crambin topological analogue	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.7；292 K；100 mM imidazole, 200 mM Zinc chloride, 7.5% Ethanol, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 292K	Resolution 1.08 Å R-free 0.176
4FC1 Ultra-high resolution neutron structure of crambin at room-temperature Deposited 2012-05-23	Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–46（46 aa）	Not recorded	No recorded non-water small molecule	NEUTRON DIFFRACTION X-ray crystallization conditions 290 K；large crystals were obtained in 1mL crystallization drops containing 20-30mg/ml protein in 80% EtOH/H2O; the well solution was 60% EtOH/H2O; no mixing with well solution was done., VAPOR DIFFUSION, SITTING DROP, temperature 290K	Resolution 1.10 Å R-free 0.253
9EWK Solvent organization in ultrahigh-resolution protein crystal structure at room temperature Deposited 2024-04-04	Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–46（46 aa）	Not recorded	EOH ETHANOL × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；289 K；80% (v/v) ethanol:water solution	Resolution 0.70 Å

26 other PDB entries and 27 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	CRAM_CRAAB
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–46； UniProt 1–46

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

P(r) Distance Distribution P(r) Distribution

Download Download SAXS (.dat) P(r) (.pr.out) CIF

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	9pis
Deposition date deposition_date	2025-07-11
Structure title title	Ab initio structure of crambin by MicroED at 0.85A
Keywords keywords	Seed protein, PLANT PROTEIN; PLANT PROTEIN
Experimental Method method	ELECTRON CRYSTALLOGRAPHY

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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier	10.57 Å
Radius of gyration Rg (electron density) rg_electron	9.72 Å
Forward intensity I(0) i0	606875.00
Molecular weight molecular_weight	4709.0 kDa
Excluded volume excluded_volume	5771 Å³
Envelope volume envelope_volume	6209 Å³
Hydration-shell volume shell_volume	5926 Å³
Envelope diameter envelope_diameter	32.2 Å
Shell Rg shell_rg	14.42 Å
Envelope Rg envelope_rg	10.05 Å
Shape Rg shape_rg	9.74 Å
Total Rg total_rg	11.04 Å
Total atoms total_atoms	638
Residues n_residues	46
Spherical-harmonic order n_harmonics	20
q range q_range	— – 0.5000 Å⁻¹
Data points n_points	101
Shell type shell_type	directional
Solvent electron density solvent_density	0.3340 e/Å³
Shell contrast contrast_shell	0.0300 e/Å³
CRYSOL version crysol_version	4.1.3

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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax	35.8 Å
Rg (real space) rg_real	10.56 Å
Rg uncertainty (real space) rg_real_error	0.29 Å
I(0) (real space) i0_real	6.0690e+05
I(0) uncertainty (real space) i0_real_error	5.9700e+03
Rg (reciprocal space) rg_reciprocal	10.56 Å
I(0) (reciprocal space) i0_reciprocal	606900.0000
Solution quality estimate total_estimate	0.8808
Solution quality rating solution_quality	GOOD a GOOD solution
P(r) peaks n_peaks	2
Primary peak position r_peak_primary	12.2 Å
Skewness Skewness skewness	0.305
Kurtosis Kurtosis kurtosis	-0.239
Angular range angular_range	— – 0.5000 Å⁻¹
Current regularization parameter α current_alpha	0.0000
Highest regularization parameter α highest_alpha	77250.0000
Real-space data points n_real_points	80
GNOM version gnom_version	4.1.3
Quality Criteria quality_criteria	AN1: 0.000; Oscil: 0.834; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.971; Smooth: 0.972

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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (2)

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8. Citations (2)

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Unit-cell length a length_a	41.490 Å
Unit-cell length b length_b	18.790 Å
Unit-cell length c length_c	22.520 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.83 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	2

Space group space_group	P 1 21 1
Space-group number int_tables_number	4
Crystal system crystal_system	—

Resolution resolution	0.850 Å
Low-resolution limit resolution_low	22.518 Å
R factor r_factor	—
R_work r_work	0.1554
R_free r_free	0.1641

Resolution resolution	0.85 Å
Resolution测定方法 resolution_method	—
颗粒数 num_particles	—

Description description	Crambin
Molecular weight formula_weight	4728.4 kDa
Source method src_method	nat
Copy count entity_count	1

Ab initio structure of crambin by MicroED at 0.85A

1. Protein Identity and Related Structures Protein Identity & Related Structures

Crambin

State in the Current Structure

Other States of the Same Protein in the Database

SAXS scattering curve SAXS Profile

P(r) Distance Distribution P(r) Distribution

2. Structure Basics 2. Structure Basics

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

5. Crystallography and Experiment 5. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

冷冻电镜 EM 3D

6. Entities and Polymers Entities & Polymers (2)

Entity 1 Entity 1 polymer

Polymer Polymer

Entity 2 Entity 2 water

8. Citations (2)

9. Files and Curves (10)

Files (7)

Curves (3)

Polymer type type	polypeptide(L)
Chain IDs strand_id	A
Atom count atom_count	792

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	71

fit	/app/data/saxs_computation/pi/9pis/9pis.fit
pddf	/app/data/pr_computation/pi/9pis/9pis.pr.out
profile	/app/data/saxs_computation/pi/9pis/9pis.dat