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9qfo

Cryo-EM structure of the cofilactin filament pointed end

Method: ELECTRON MICROSCOPY D_max: 185.6 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9qfo
deposition_date	2025-03-12
title	Cryo-EM structure of the cofilactin filament pointed end
keywords	actin, cofilin, pointed end, filament, cytoskeleton, STRUCTURAL PROTEIN; STRUCTURAL PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	52.82 Å
rg_electron	53.08 Å
i0	1423680000.00
molecular_weight	316000.0 kDa
excluded_volume	396020 Å³
envelope_volume	537550 Å³
shell_volume	87040 Å³
envelope_diameter	202.2 Å
shell_rg	53.64 Å
envelope_rg	52.46 Å
shape_rg	53.09 Å
total_rg	53.05 Å
total_atoms	22156
n_residues	2808
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	185.6 Å
rg_real	53.12 Å
rg_real_error	1.82 Å
i0_real	1.4240e+09
i0_real_error	2.5450e+07
rg_reciprocal	52.57 Å
i0_reciprocal	1423000000.0000
total_estimate	0.8410
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	55.7 Å
skewness	0.553
kurtosis	-0.117
angular_range	— – 0.1500 Å⁻¹
current_alpha	0.0000
highest_alpha	124200000.0000
n_real_points	31
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.756; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.991; Smooth: 0.670

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	Cofilin-1
formula_weight	18532.5 kDa
src_method	man
entity_count	4

Polymer

type	polypeptide(L)
strand_id	G,H,I,J
atom_count	22156

MASGVAVSDGVIKVFNDMKVRKSSTPEEVKKRKKAVLFCLSEDKKNIILEEGKEILVGDVGQTVDDPYATFVKMLPDKDCRYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIYASSKDAIKKKLTGIKHELQANCYEEVKDRCTLAEKLGGSAVISLEGKPL

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Escherichia coli BL21(DE3)

Entity 2 polymer

description	Actin, cytoplasmic 1, N-terminally processed
formula_weight	41632.4 kDa
mutation	C272A
src_method	man
entity_count	6

Polymer

type	polypeptide(L)
strand_id	A,B,C,D,E,F

DDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESAGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Trichoplusia ni

Entity 3 non-polymer

description	ADENOSINE-5'-DIPHOSPHATE
formula_weight	427.2 kDa
src_method	syn
entity_count	6

Entity 4 non-polymer

description	MAGNESIUM ION
formula_weight	24.3 kDa
src_method	syn
entity_count	6

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	triclinic

resolution	2.96 Å
resolution_method	—
num_particles	151075

fit	/app/data/saxs_computation/qf/9qfo/9qfo.fit
pddf	/app/data/pr_computation/qf/9qfo/9qfo.pr.out
profile	/app/data/saxs_computation/qf/9qfo/9qfo.dat

Cryo-EM structure of the cofilactin filament pointed end

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

EM 3D

5. Entities & Polymer Info (4)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 non-polymer

Entity 4 non-polymer

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)