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9qt4

CryoEM structure of Arabidopsis TIR-NLR WRR4A tetramer in complex with effector CCG40 (focused refinement)

Method: ELECTRON MICROSCOPY D_max: 182.4 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9qt4
deposition_date	2025-04-07
title	CryoEM structure of Arabidopsis TIR-NLR WRR4A tetramer in complex with effector CCG40 (focused refinement)
keywords	TIR NLR, Arabidopsis, Resistosome, Effector, Oomycete, PLANT PROTEIN; PLANT PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	58.37 Å
rg_electron	58.39 Å
i0	1518840000.00
molecular_weight	330930.0 kDa
excluded_volume	416530 Å³
envelope_volume	600110 Å³
shell_volume	89729 Å³
envelope_diameter	194.4 Å
shell_rg	55.96 Å
envelope_rg	57.01 Å
shape_rg	58.42 Å
total_rg	58.24 Å
total_atoms	23228
n_residues	2944
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	182.4 Å
rg_real	58.40 Å
rg_real_error	1.37 Å
i0_real	1.5190e+09
i0_real_error	2.9070e+07
rg_reciprocal	58.32 Å
i0_reciprocal	1519000000.0000
total_estimate	0.8546
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	67.6 Å
skewness	0.267
kurtosis	-0.411
angular_range	— – 0.1350 Å⁻¹
current_alpha	0.0000
highest_alpha	65990000.0000
n_real_points	28
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.955; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.999; Smooth: 0.242

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4. Crystallography & Experiment

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5. Entities & Polymer Info (2)

Entity 1 polymer

description	CCG40
formula_weight	24496.6 kDa
src_method	man
entity_count	4

Polymer

type	polypeptide(L)
strand_id	P,Q,R,S
atom_count	23228

MAYRLFLTLWVYQIYHVVGVLKSGELRIKKTDSRNSLSLCQACVLNKLGASRMKLINDDEEVATYKITGSDFVFANLKVDCSGVNECNIDKIIPLSWPSMMPNEIALSARSPQLYVSASESSSSVLCMDCLQENAAARFISKFRVNKLKVVRIVYMIYGALLNTQHLKCTDYCPGLTILEEMDNSKQLVQIFRSEEHLPLSRIRHHVIQCNALSHK

Source

organism	Albugo candida
ncbi_taxonomy_id	65357
host_organism	Nicotiana benthamiana

Entity 2 polymer

description	Disease resistance protein ADR2
formula_weight	114119.0 kDa
ec_number	3.2.2.6
src_method	man
entity_count	4

Polymer

type	polypeptide(L)
strand_id	A,B,C,D

MASSSSSPRNWRYNVFTSFHGPDVRIKFLSHLRQQFVYNGITMFDDNGIERSQIIAPALKKAIGESRVAIVLLSKNYASSSWCLDELLEILKCKEYIGQIVMTVFYEVDPSHVRKQTGDFGIAFKETCAHKTEEERSKWSQALTYVGNIAGEDFIHWKDEAKMIEKIARDVSTKINVTPCRDFDDMVGLERHLKEMVSLLDLDKEGVKMVGISGPAGIGKSTIAKALHSRHSSTFQHNCFVDNLWENYKICTGEHGVKLRLHEQFVSKILKQNGLELTHLSVIKDRLQDKKVLIILDDVESLAQLETLADMTWFGPGSRVIVTTENKEILQQHGIGDIYQVGYPSESEALTIFCLSAFKQASPPDGFMDLADEVVRICDKLPLALCVLGSSLLRKSQTDWEDELPRLRNCLDGIESVLKVGFESLNEKDQALFLYITVFFNYECADHVTLMLAKSNLNVRLGLKNLANRYLIHIDHDQKKRVVVHRLLRVMAIQVCTKQKPWKSQILVDAEKIAYVLEEATGNRSIKGVSFDTAEIDELMISPKAFEKMCNLLFLKVYDAGWHTGKRKLDIPEDIKFPRTIRLFHWDAYSGKRLPSSFFAENLVEVNMQDSELQKLWEGTQCLANLKKIDLSRSSCLTELPDLSNATNLEDLYVGSCTALVELPSSIGNLHKLAHIMMYSCESLEVIPSLINLTSLTFLNMNKCSRLRRFPDIPTSIEDVQVTGTTLEELPASLTHCSGLQTIKISGSVNLKIFYTELPVSVSHINISNSGIEWITEDCIKGLHNLHDLCLSGCKRLVSLPELPRSLKILQADDCDSLESLNGHLNTPNAELYFANCFKLDAEARRAIIQQSFVSGWALLPGLEVPPEFGHRARGNSLIIPYSASNRFKVCVVMSLNHHQPFELVPRNLLYRWTVIGDSVSSDEKTFHLSHMFNADSVNSKLQKPHLFIFHSCLPFIFHSCLPFIFDISNIMLEFSSEYKDFDILECGVQILTDETDERNIWGSLVF

Source

organism	Arabidopsis thaliana
common_name	thale cress
ncbi_taxonomy_id	3702
host_organism	Nicotiana benthamiana

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	4.00 Å
resolution_method	—
num_particles	34546

fit	/app/data/saxs_computation/qt/9qt4/9qt4.fit
pddf	/app/data/pr_computation/qt/9qt4/9qt4.pr.out
profile	/app/data/saxs_computation/qt/9qt4/9qt4.dat

CryoEM structure of Arabidopsis TIR-NLR WRR4A tetramer in complex with effector CCG40 (focused refinement)

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

EM 3D

5. Entities & Polymer Info (2)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)