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9r8w

Cryo-EM structure of Arabidopsis TIR-NLR WRR4A tetramer in complex with weakly bound effector CCG28 (C2-symmetry)

Method: ELECTRON MICROSCOPY D_max: 179.6 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9r8w
deposition_date	2025-05-17
title	Cryo-EM structure of Arabidopsis TIR-NLR WRR4A tetramer in complex with weakly bound effector CCG28 (C2-symmetry)
keywords	TIR NLR, Resistosome, Oomycete, effector WRR4A, PLANT PROTEIN; PLANT PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	57.63 Å
rg_electron	57.34 Å
i0	3148130000.00
molecular_weight	475380.0 kDa
excluded_volume	597250 Å³
envelope_volume	843470 Å³
shell_volume	123690 Å³
envelope_diameter	194.7 Å
shell_rg	58.76 Å
envelope_rg	55.96 Å
shape_rg	57.36 Å
total_rg	57.30 Å
total_atoms	33382
n_residues	4202
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	179.6 Å
rg_real	57.37 Å
rg_real_error	1.17 Å
i0_real	3.1480e+09
i0_real_error	6.8410e+07
rg_reciprocal	57.82 Å
i0_reciprocal	3150000000.0000
total_estimate	0.8823
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	73.2 Å
skewness	0.145
kurtosis	-0.384
angular_range	— – 0.1350 Å⁻¹
current_alpha	0.0000
highest_alpha	179700000.0000
n_real_points	28
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.920; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.972; Smooth: 0.733

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	Effector CCG28
formula_weight	61221.6 kDa
src_method	man
entity_count	4

Polymer

type	polypeptide(L)
strand_id	Q,R,S,P
atom_count	33382

MYLSLLSCRIMVHQHWLLFSFLWLLTAANQRGIITVEFNHKSDLSRCQSCLVDIAGLDRLSLTSISTNDLSIKIAKFQASGQACSFISAKLDCRHQTKCGSVTVTILEHVESLASSSNLQIAVNEQLKPLCILTSVQTSFDCGLCLRMTSSSEGIQKLKLITMDTKEYYLHVLHTHQRNIFEPCSLSGVCSGIQYEFDAEYCDHLVKKLFVPRPYSSEHVIFPSSVDYTGPQELASAVRAIHCTVKWELQCIQFITSQKPRRNCLACMSDVSSRMALLVALSSIEYGYEGYREGDTQYLFFVIVQAAFDFYLQCDETCGRITFHSLDSCISLAIKTNNVSPSQGKIYYNMVQRDLKKKEAVQETMLIPQTSSCMWVEHELGEREWCKVCLQHLSKIDAKILSSIDFLVLSEESLHTISGCISFGSELGMKLCKKVTPIPDQFCHSFDMLPSSHGYDIMAPSLPKIKHLVSTGRPKTPDIDCFTCLTSTLDILITDLVRFWMVGFPEESTCYSTCGTLKMDDRQVLDISMIRLMSSNTIGSRFN

Source

organism	Albugo candida
ncbi_taxonomy_id	65357
host_organism	Nicotiana benthamiana

Entity 2 polymer

description	Disease resistance protein ADR2
formula_weight	114119.0 kDa
ec_number	3.2.2.6
src_method	man
entity_count	4

Polymer

type	polypeptide(L)
strand_id	A,B,C,D

MASSSSSPRNWRYNVFTSFHGPDVRIKFLSHLRQQFVYNGITMFDDNGIERSQIIAPALKKAIGESRVAIVLLSKNYASSSWCLDELLEILKCKEYIGQIVMTVFYEVDPSHVRKQTGDFGIAFKETCAHKTEEERSKWSQALTYVGNIAGEDFIHWKDEAKMIEKIARDVSTKINVTPCRDFDDMVGLERHLKEMVSLLDLDKEGVKMVGISGPAGIGKSTIAKALHSRHSSTFQHNCFVDNLWENYKICTGEHGVKLRLHEQFVSKILKQNGLELTHLSVIKDRLQDKKVLIILDDVESLAQLETLADMTWFGPGSRVIVTTENKEILQQHGIGDIYQVGYPSESEALTIFCLSAFKQASPPDGFMDLADEVVRICDKLPLALCVLGSSLLRKSQTDWEDELPRLRNCLDGIESVLKVGFESLNEKDQALFLYITVFFNYECADHVTLMLAKSNLNVRLGLKNLANRYLIHIDHDQKKRVVVHRLLRVMAIQVCTKQKPWKSQILVDAEKIAYVLEEATGNRSIKGVSFDTAEIDELMISPKAFEKMCNLLFLKVYDAGWHTGKRKLDIPEDIKFPRTIRLFHWDAYSGKRLPSSFFAENLVEVNMQDSELQKLWEGTQCLANLKKIDLSRSSCLTELPDLSNATNLEDLYVGSCTALVELPSSIGNLHKLAHIMMYSCESLEVIPSLINLTSLTFLNMNKCSRLRRFPDIPTSIEDVQVTGTTLEELPASLTHCSGLQTIKISGSVNLKIFYTELPVSVSHINISNSGIEWITEDCIKGLHNLHDLCLSGCKRLVSLPELPRSLKILQADDCDSLESLNGHLNTPNAELYFANCFKLDAEARRAIIQQSFVSGWALLPGLEVPPEFGHRARGNSLIIPYSASNRFKVCVVMSLNHHQPFELVPRNLLYRWTVIGDSVSSDEKTFHLSHMFNADSVNSKLQKPHLFIFHSCLPFIFHSCLPFIFDISNIMLEFSSEYKDFDILECGVQILTDETDERNIWGSLVF

Source

organism	Arabidopsis thaliana
common_name	thale cress
ncbi_taxonomy_id	3702
host_organism	Nicotiana benthamiana

Entity 3 non-polymer

description	ADENOSINE-5'-DIPHOSPHATE
formula_weight	427.2 kDa
src_method	syn
entity_count	4

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	3.500 Å
resolution_low	— Å
r_factor	—
r_work	—
r_free	—

resolution	3.50 Å
resolution_method	—
num_particles	98507

fit	/app/data/saxs_computation/r8/9r8w/9r8w.fit
pddf	/app/data/pr_computation/r8/9r8w/9r8w.pr.out
profile	/app/data/saxs_computation/r8/9r8w/9r8w.dat

Cryo-EM structure of Arabidopsis TIR-NLR WRR4A tetramer in complex with weakly bound effector CCG28 (C2-symmetry)

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

EM 3D

5. Entities & Polymer Info (3)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 non-polymer

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)