SAXSdb — 9rlg

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1. Structure Basics

entry_id	9rlg
deposition_date	2025-06-16
title	LolCDE complex with LolB lipoprotein
keywords	Gram-negative, envelope biogenesis, outer membrane protein, PROTEIN TRANSPORT; PROTEIN TRANSPORT
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	46.66 Å
rg_electron	45.95 Å
i0	357299000.00
molecular_weight	159610.0 kDa
excluded_volume	201660 Å³
envelope_volume	286190 Å³
shell_volume	53337 Å³
envelope_diameter	143.4 Å
shell_rg	48.71 Å
envelope_rg	45.04 Å
shape_rg	45.96 Å
total_rg	46.04 Å
total_atoms	11215
n_residues	1448
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	132.2 Å
rg_real	46.89 Å
rg_real_error	1.05 Å
i0_real	3.5730e+08
i0_real_error	6.5480e+06
rg_reciprocal	46.67 Å
i0_reciprocal	357200000.0000
total_estimate	0.8100
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	38.9 Å
skewness	0.280
kurtosis	-0.941
angular_range	— – 0.1700 Å⁻¹
current_alpha	0.0000
highest_alpha	30650000.0000
n_real_points	35
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.917; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.775; Smooth: 0.000

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4. Crystallography & Experiment

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5. Entities & Polymer Info (6)

Entity 1 polymer

description	Lipoprotein-releasing system transmembrane protein LolC
formula_weight	43295.5 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	C
atom_count	11215

MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTVLSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVVLQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGDQIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGNITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVAVAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGALLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQPAEALRYE

Source

organism	Escherichia coli K-12
ncbi_taxonomy_id	83333
host_organism	Escherichia coli BL21(DE3)

Entity 2 polymer

description	Lipoprotein-releasing system transmembrane protein LolE
formula_weight	45386.0 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	E

MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVGLSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDHVQKVPGIAAAAPYINFTGLVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKVKQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMGSSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAMVLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCGVIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSLLASWYPARRASNIDPARVLSGQ

Source

organism	Escherichia coli K-12
ncbi_taxonomy_id	83333
host_organism	Escherichia coli BL21(DE3)

Entity 3 polymer

description	Lipoprotein-releasing system ATP-binding protein LolD
formula_weight	26444.3 kDa
ec_number	7.6.2.-
mutation	C-terminal His tag (GSHHHHHH)
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	D,F

MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSSGSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDFTALENVAMPLLIGKKKPAEINSRALEMLKAVGLDHRANHRPSELSGGERQRVAIARALVNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEMRDGRLTAELSLMGAEGSHHHHHH

Source

organism	Escherichia coli K-12
ncbi_taxonomy_id	83333
host_organism	Escherichia coli BL21(DE3)

Entity 4 polymer

description	Outer-membrane lipoprotein LolB
formula_weight	25429.1 kDa
mutation	C-terminal GS linker TEV twin strep tag (GSAWSHPQFEK)
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	L

CSVTTPKGPGKSPDSPQWRQHQQDVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGNVQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEITYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVKGSGENLYFQSGSAWSHPQFEKGGGSGGGSGGSAWSHPQFEK

Source

organism	Escherichia coli K-12
ncbi_taxonomy_id	83333
host_organism	Escherichia coli BL21(DE3)

Entity 5 non-polymer

description	PALMITIC ACID
formula_weight	256.4 kDa
src_method	syn
entity_count	1

Entity 6 non-polymer

description	(2S)-3-hydroxypropane-1,2-diyl dihexadecanoate
formula_weight	568.9 kDa
src_method	syn
entity_count	1

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	triclinic

resolution	3.09 Å
resolution_method	—
num_particles	316566

fit	/app/data/saxs_computation/rl/9rlg/9rlg.fit
pddf	/app/data/pr_computation/rl/9rlg/9rlg.pr.out
profile	/app/data/saxs_computation/rl/9rlg/9rlg.dat

LolCDE complex with LolB lipoprotein

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

EM 3D

5. Entities & Polymer Info (6)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 polymer

Polymer

Source

Entity 4 polymer

Polymer

Source

Entity 5 non-polymer

Entity 6 non-polymer

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)