9taj

E. coli Complex I WT purified in LMNG

Method: ELECTRON MICROSCOPY

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1. Protein Identity and Related Structures Protein Identity & Related Structures

NADH-quinone oxidoreductase subunit E

Escherichia coli BW25113

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 13	NADH-quinone oxidoreductase subunit F × 1 （P31979） NADH-quinone oxidoreductase subunit G × 1 （P33602） NADH-quinone oxidoreductase subunit I × 1 （P0AFD6） NADH-quinone oxidoreductase subunit B × 1 （P0AFC7） NADH-quinone oxidoreductase subunit C/D × 1 （P33599） NADH-quinone oxidoreductase subunit H × 1 （P0AFD4） NADH-quinone oxidoreductase subunit J × 1 （P0AFE0） NADH-quinone oxidoreductase subunit K × 1 （P0AFE4） NADH-quinone oxidoreductase subunit M × 1 （P0AFE8） NADH-quinone oxidoreductase subunit A × 1 （P0AFC3） NADH-quinone oxidoreductase subunit L × 1 （P33607） NADH-quinone oxidoreductase subunit N × 1 （P0AFF0） FE2/S2 (INORGANIC) CLUSTER × 2 CALCIUM ION × 5 FLAVIN MONONUCLEOTIDE × 1 IRON/SULFUR CLUSTER × 7 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 EICOSANE × 2 CARDIOLIPIN × 1 TRIDECANE × 3 Ubiquinone-8 × 2 water × 13	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	NUOE_ECOLI
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain E； PDBConstruct 1–166； UniProt 1–166

NADH-quinone oxidoreductase subunit F

Escherichia coli BW25113

UniProt P31979

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 13	NADH-quinone oxidoreductase subunit E × 1 （P0AFD1） NADH-quinone oxidoreductase subunit G × 1 （P33602） NADH-quinone oxidoreductase subunit I × 1 （P0AFD6） NADH-quinone oxidoreductase subunit B × 1 （P0AFC7） NADH-quinone oxidoreductase subunit C/D × 1 （P33599） NADH-quinone oxidoreductase subunit H × 1 （P0AFD4） NADH-quinone oxidoreductase subunit J × 1 （P0AFE0） NADH-quinone oxidoreductase subunit K × 1 （P0AFE4） NADH-quinone oxidoreductase subunit M × 1 （P0AFE8） NADH-quinone oxidoreductase subunit A × 1 （P0AFC3） NADH-quinone oxidoreductase subunit L × 1 （P33607） NADH-quinone oxidoreductase subunit N × 1 （P0AFF0） FE2/S2 (INORGANIC) CLUSTER × 2 CALCIUM ION × 5 FLAVIN MONONUCLEOTIDE × 1 IRON/SULFUR CLUSTER × 7 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 EICOSANE × 2 CARDIOLIPIN × 1 TRIDECANE × 3 Ubiquinone-8 × 2 water × 13	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	NUOF_ECOLI
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain F； PDBConstruct 18–461； UniProt 2–445

NADH-quinone oxidoreductase subunit G

Escherichia coli BW25113

UniProt P33602

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 13	NADH-quinone oxidoreductase subunit E × 1 （P0AFD1） NADH-quinone oxidoreductase subunit F × 1 （P31979） NADH-quinone oxidoreductase subunit I × 1 （P0AFD6） NADH-quinone oxidoreductase subunit B × 1 （P0AFC7） NADH-quinone oxidoreductase subunit C/D × 1 （P33599） NADH-quinone oxidoreductase subunit H × 1 （P0AFD4） NADH-quinone oxidoreductase subunit J × 1 （P0AFE0） NADH-quinone oxidoreductase subunit K × 1 （P0AFE4） NADH-quinone oxidoreductase subunit M × 1 （P0AFE8） NADH-quinone oxidoreductase subunit A × 1 （P0AFC3） NADH-quinone oxidoreductase subunit L × 1 （P33607） NADH-quinone oxidoreductase subunit N × 1 （P0AFF0） FE2/S2 (INORGANIC) CLUSTER × 2 CALCIUM ION × 5 FLAVIN MONONUCLEOTIDE × 1 IRON/SULFUR CLUSTER × 7 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 EICOSANE × 2 CARDIOLIPIN × 1 TRIDECANE × 3 Ubiquinone-8 × 2 water × 13	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	NUOG_ECOLI
Isoform	—
PDB entities	3
Chains and sequence ranges	Author chain G； PDBConstruct 3–910； UniProt 1–908

NADH-quinone oxidoreductase subunit I

Escherichia coli BL21(DE3)

UniProt P0AFD6

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 13	NADH-quinone oxidoreductase subunit E × 1 （P0AFD1） NADH-quinone oxidoreductase subunit F × 1 （P31979） NADH-quinone oxidoreductase subunit G × 1 （P33602） NADH-quinone oxidoreductase subunit B × 1 （P0AFC7） NADH-quinone oxidoreductase subunit C/D × 1 （P33599） NADH-quinone oxidoreductase subunit H × 1 （P0AFD4） NADH-quinone oxidoreductase subunit J × 1 （P0AFE0） NADH-quinone oxidoreductase subunit K × 1 （P0AFE4） NADH-quinone oxidoreductase subunit M × 1 （P0AFE8） NADH-quinone oxidoreductase subunit A × 1 （P0AFC3） NADH-quinone oxidoreductase subunit L × 1 （P33607） NADH-quinone oxidoreductase subunit N × 1 （P0AFF0） FE2/S2 (INORGANIC) CLUSTER × 2 CALCIUM ION × 5 FLAVIN MONONUCLEOTIDE × 1 IRON/SULFUR CLUSTER × 7 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 EICOSANE × 2 CARDIOLIPIN × 1 TRIDECANE × 3 Ubiquinone-8 × 2 water × 13	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	NUOI_ECOLI
Isoform	—
PDB entities	4
Chains and sequence ranges	Author chain I； PDBConstruct 1–180； UniProt 1–180

NADH-quinone oxidoreductase subunit B

Escherichia coli BW25113

UniProt P0AFC7

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 13	NADH-quinone oxidoreductase subunit E × 1 （P0AFD1） NADH-quinone oxidoreductase subunit F × 1 （P31979） NADH-quinone oxidoreductase subunit G × 1 （P33602） NADH-quinone oxidoreductase subunit I × 1 （P0AFD6） NADH-quinone oxidoreductase subunit C/D × 1 （P33599） NADH-quinone oxidoreductase subunit H × 1 （P0AFD4） NADH-quinone oxidoreductase subunit J × 1 （P0AFE0） NADH-quinone oxidoreductase subunit K × 1 （P0AFE4） NADH-quinone oxidoreductase subunit M × 1 （P0AFE8） NADH-quinone oxidoreductase subunit A × 1 （P0AFC3） NADH-quinone oxidoreductase subunit L × 1 （P33607） NADH-quinone oxidoreductase subunit N × 1 （P0AFF0） FE2/S2 (INORGANIC) CLUSTER × 2 CALCIUM ION × 5 FLAVIN MONONUCLEOTIDE × 1 IRON/SULFUR CLUSTER × 7 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 EICOSANE × 2 CARDIOLIPIN × 1 TRIDECANE × 3 Ubiquinone-8 × 2 water × 13	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	NUOB_ECOLI
Isoform	—
PDB entities	5
Chains and sequence ranges	Author chain B； PDBConstruct 1–220； UniProt 1–220

NADH-quinone oxidoreductase subunit C/D

Escherichia coli BL21(DE3)

UniProt P33599

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 13	NADH-quinone oxidoreductase subunit E × 1 （P0AFD1） NADH-quinone oxidoreductase subunit F × 1 （P31979） NADH-quinone oxidoreductase subunit G × 1 （P33602） NADH-quinone oxidoreductase subunit I × 1 （P0AFD6） NADH-quinone oxidoreductase subunit B × 1 （P0AFC7） NADH-quinone oxidoreductase subunit H × 1 （P0AFD4） NADH-quinone oxidoreductase subunit J × 1 （P0AFE0） NADH-quinone oxidoreductase subunit K × 1 （P0AFE4） NADH-quinone oxidoreductase subunit M × 1 （P0AFE8） NADH-quinone oxidoreductase subunit A × 1 （P0AFC3） NADH-quinone oxidoreductase subunit L × 1 （P33607） NADH-quinone oxidoreductase subunit N × 1 （P0AFF0） FE2/S2 (INORGANIC) CLUSTER × 2 CALCIUM ION × 5 FLAVIN MONONUCLEOTIDE × 1 IRON/SULFUR CLUSTER × 7 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 EICOSANE × 2 CARDIOLIPIN × 1 TRIDECANE × 3 Ubiquinone-8 × 2 water × 13	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	NUOCD_ECOLI
Isoform	—
PDB entities	6
Chains and sequence ranges	Author chain C； PDBConstruct 5–600； UniProt 1–596

NADH-quinone oxidoreductase subunit H

Escherichia coli BW25113

UniProt P0AFD4

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 13	NADH-quinone oxidoreductase subunit E × 1 （P0AFD1） NADH-quinone oxidoreductase subunit F × 1 （P31979） NADH-quinone oxidoreductase subunit G × 1 （P33602） NADH-quinone oxidoreductase subunit I × 1 （P0AFD6） NADH-quinone oxidoreductase subunit B × 1 （P0AFC7） NADH-quinone oxidoreductase subunit C/D × 1 （P33599） NADH-quinone oxidoreductase subunit J × 1 （P0AFE0） NADH-quinone oxidoreductase subunit K × 1 （P0AFE4） NADH-quinone oxidoreductase subunit M × 1 （P0AFE8） NADH-quinone oxidoreductase subunit A × 1 （P0AFC3） NADH-quinone oxidoreductase subunit L × 1 （P33607） NADH-quinone oxidoreductase subunit N × 1 （P0AFF0） FE2/S2 (INORGANIC) CLUSTER × 2 CALCIUM ION × 5 FLAVIN MONONUCLEOTIDE × 1 IRON/SULFUR CLUSTER × 7 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 EICOSANE × 2 CARDIOLIPIN × 1 TRIDECANE × 3 Ubiquinone-8 × 2 water × 13	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	NUOH_ECOLI
Isoform	—
PDB entities	7
Chains and sequence ranges	Author chain H； PDBConstruct 1–325； UniProt 1–325

NADH-quinone oxidoreductase subunit J

Escherichia coli BW25113

UniProt P0AFE0

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 13	NADH-quinone oxidoreductase subunit E × 1 （P0AFD1） NADH-quinone oxidoreductase subunit F × 1 （P31979） NADH-quinone oxidoreductase subunit G × 1 （P33602） NADH-quinone oxidoreductase subunit I × 1 （P0AFD6） NADH-quinone oxidoreductase subunit B × 1 （P0AFC7） NADH-quinone oxidoreductase subunit C/D × 1 （P33599） NADH-quinone oxidoreductase subunit H × 1 （P0AFD4） NADH-quinone oxidoreductase subunit K × 1 （P0AFE4） NADH-quinone oxidoreductase subunit M × 1 （P0AFE8） NADH-quinone oxidoreductase subunit A × 1 （P0AFC3） NADH-quinone oxidoreductase subunit L × 1 （P33607） NADH-quinone oxidoreductase subunit N × 1 （P0AFF0） FE2/S2 (INORGANIC) CLUSTER × 2 CALCIUM ION × 5 FLAVIN MONONUCLEOTIDE × 1 IRON/SULFUR CLUSTER × 7 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 EICOSANE × 2 CARDIOLIPIN × 1 TRIDECANE × 3 Ubiquinone-8 × 2 water × 13	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	NUOJ_ECOLI
Isoform	—
PDB entities	8
Chains and sequence ranges	Author chain J； PDBConstruct 1–184； UniProt 1–184

NADH-quinone oxidoreductase subunit K

Escherichia coli BW25113

UniProt P0AFE4

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 13	NADH-quinone oxidoreductase subunit E × 1 （P0AFD1） NADH-quinone oxidoreductase subunit F × 1 （P31979） NADH-quinone oxidoreductase subunit G × 1 （P33602） NADH-quinone oxidoreductase subunit I × 1 （P0AFD6） NADH-quinone oxidoreductase subunit B × 1 （P0AFC7） NADH-quinone oxidoreductase subunit C/D × 1 （P33599） NADH-quinone oxidoreductase subunit H × 1 （P0AFD4） NADH-quinone oxidoreductase subunit J × 1 （P0AFE0） NADH-quinone oxidoreductase subunit M × 1 （P0AFE8） NADH-quinone oxidoreductase subunit A × 1 （P0AFC3） NADH-quinone oxidoreductase subunit L × 1 （P33607） NADH-quinone oxidoreductase subunit N × 1 （P0AFF0） FE2/S2 (INORGANIC) CLUSTER × 2 CALCIUM ION × 5 FLAVIN MONONUCLEOTIDE × 1 IRON/SULFUR CLUSTER × 7 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 EICOSANE × 2 CARDIOLIPIN × 1 TRIDECANE × 3 Ubiquinone-8 × 2 water × 13	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	NUOK_ECOLI
Isoform	—
PDB entities	9
Chains and sequence ranges	Author chain K； PDBConstruct 1–100； UniProt 1–100

NADH-quinone oxidoreductase subunit M

Escherichia coli BW25113

UniProt P0AFE8

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 13	NADH-quinone oxidoreductase subunit E × 1 （P0AFD1） NADH-quinone oxidoreductase subunit F × 1 （P31979） NADH-quinone oxidoreductase subunit G × 1 （P33602） NADH-quinone oxidoreductase subunit I × 1 （P0AFD6） NADH-quinone oxidoreductase subunit B × 1 （P0AFC7） NADH-quinone oxidoreductase subunit C/D × 1 （P33599） NADH-quinone oxidoreductase subunit H × 1 （P0AFD4） NADH-quinone oxidoreductase subunit J × 1 （P0AFE0） NADH-quinone oxidoreductase subunit K × 1 （P0AFE4） NADH-quinone oxidoreductase subunit A × 1 （P0AFC3） NADH-quinone oxidoreductase subunit L × 1 （P33607） NADH-quinone oxidoreductase subunit N × 1 （P0AFF0） FE2/S2 (INORGANIC) CLUSTER × 2 CALCIUM ION × 5 FLAVIN MONONUCLEOTIDE × 1 IRON/SULFUR CLUSTER × 7 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 EICOSANE × 2 CARDIOLIPIN × 1 TRIDECANE × 3 Ubiquinone-8 × 2 water × 13	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	NUOM_ECOLI
Isoform	—
PDB entities	10
Chains and sequence ranges	Author chain M； PDBConstruct 1–509； UniProt 1–509

NADH-quinone oxidoreductase subunit A

Escherichia coli BW25113

UniProt P0AFC3

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 13	NADH-quinone oxidoreductase subunit E × 1 （P0AFD1） NADH-quinone oxidoreductase subunit F × 1 （P31979） NADH-quinone oxidoreductase subunit G × 1 （P33602） NADH-quinone oxidoreductase subunit I × 1 （P0AFD6） NADH-quinone oxidoreductase subunit B × 1 （P0AFC7） NADH-quinone oxidoreductase subunit C/D × 1 （P33599） NADH-quinone oxidoreductase subunit H × 1 （P0AFD4） NADH-quinone oxidoreductase subunit J × 1 （P0AFE0） NADH-quinone oxidoreductase subunit K × 1 （P0AFE4） NADH-quinone oxidoreductase subunit M × 1 （P0AFE8） NADH-quinone oxidoreductase subunit L × 1 （P33607） NADH-quinone oxidoreductase subunit N × 1 （P0AFF0） FE2/S2 (INORGANIC) CLUSTER × 2 CALCIUM ION × 5 FLAVIN MONONUCLEOTIDE × 1 IRON/SULFUR CLUSTER × 7 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 EICOSANE × 2 CARDIOLIPIN × 1 TRIDECANE × 3 Ubiquinone-8 × 2 water × 13	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	NUOA_ECOLI
Isoform	—
PDB entities	11
Chains and sequence ranges	Author chain A； PDBConstruct 1–147； UniProt 1–147

NADH-quinone oxidoreductase subunit L

Escherichia coli BW25113

UniProt P33607

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 13	NADH-quinone oxidoreductase subunit E × 1 （P0AFD1） NADH-quinone oxidoreductase subunit F × 1 （P31979） NADH-quinone oxidoreductase subunit G × 1 （P33602） NADH-quinone oxidoreductase subunit I × 1 （P0AFD6） NADH-quinone oxidoreductase subunit B × 1 （P0AFC7） NADH-quinone oxidoreductase subunit C/D × 1 （P33599） NADH-quinone oxidoreductase subunit H × 1 （P0AFD4） NADH-quinone oxidoreductase subunit J × 1 （P0AFE0） NADH-quinone oxidoreductase subunit K × 1 （P0AFE4） NADH-quinone oxidoreductase subunit M × 1 （P0AFE8） NADH-quinone oxidoreductase subunit A × 1 （P0AFC3） NADH-quinone oxidoreductase subunit N × 1 （P0AFF0） FE2/S2 (INORGANIC) CLUSTER × 2 CALCIUM ION × 5 FLAVIN MONONUCLEOTIDE × 1 IRON/SULFUR CLUSTER × 7 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 EICOSANE × 2 CARDIOLIPIN × 1 TRIDECANE × 3 Ubiquinone-8 × 2 water × 13	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	NUOL_ECOLI
Isoform	—
PDB entities	12
Chains and sequence ranges	Author chain L； PDBConstruct 1–613； UniProt 1–613

NADH-quinone oxidoreductase subunit N

Escherichia coli BW25113

UniProt P0AFF0

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 13	NADH-quinone oxidoreductase subunit E × 1 （P0AFD1） NADH-quinone oxidoreductase subunit F × 1 （P31979） NADH-quinone oxidoreductase subunit G × 1 （P33602） NADH-quinone oxidoreductase subunit I × 1 （P0AFD6） NADH-quinone oxidoreductase subunit B × 1 （P0AFC7） NADH-quinone oxidoreductase subunit C/D × 1 （P33599） NADH-quinone oxidoreductase subunit H × 1 （P0AFD4） NADH-quinone oxidoreductase subunit J × 1 （P0AFE0） NADH-quinone oxidoreductase subunit K × 1 （P0AFE4） NADH-quinone oxidoreductase subunit M × 1 （P0AFE8） NADH-quinone oxidoreductase subunit A × 1 （P0AFC3） NADH-quinone oxidoreductase subunit L × 1 （P33607） FE2/S2 (INORGANIC) CLUSTER × 2 CALCIUM ION × 5 FLAVIN MONONUCLEOTIDE × 1 IRON/SULFUR CLUSTER × 7 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 EICOSANE × 2 CARDIOLIPIN × 1 TRIDECANE × 3 Ubiquinone-8 × 2 water × 13	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	NUON_ECOLI
Isoform	—
PDB entities	13
Chains and sequence ranges	Author chain N； PDBConstruct 1–485； UniProt 1–485

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	9taj
Deposition date deposition_date	2025-11-18
Structure title title	E. coli Complex I WT purified in LMNG
Keywords keywords	PROTON TRANSPORT, bioenergetics; PROTON TRANSPORT
Experimental Method method	ELECTRON MICROSCOPY

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

9taj__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

9taj__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

9taj__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	78.29 Å
Rg (electron density)	80.05 Å
Total Rg	79.88 Å
Atom count	37747
Residues	4698
Excluded volume	686970 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`9taj__assembly_1__model_1`	13-meric （13）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (23)

Entity 1 Entity 1 polymer

Description description	NADH-quinone oxidoreductase subunit E
Molecular weight formula_weight	18614.0 kDa
EC 编号 ec_number	7.1.1.-
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	E
Atom count atom_count	38186

MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQKQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGYQGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPELLERYK

Source Source

Source organism organism	Escherichia coli BW25113
NCBI taxonomy ID ncbi_taxonomy_id	679895
Host organism host_organism	Escherichia coli BW25113

Entity 2 Entity 2 polymer

Description description	NADH-quinone oxidoreductase subunit F
Molecular weight formula_weight	51208.4 kDa
EC 编号 ec_number	7.1.1.-
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	F

MRGSHHHHHHTDPALRAKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTGLSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDRLLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNIMGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNNVETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILEDYAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEINMVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLGPGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW

Source Source

Source organism organism	Escherichia coli BW25113
NCBI taxonomy ID ncbi_taxonomy_id	679895
Host organism host_organism	Escherichia coli BW25113

Entity 3 Entity 3 polymer

Description description	NADH-quinone oxidoreductase subunit G
Molecular weight formula_weight	100663.6 kDa
EC 编号 ec_number	7.1.1.-
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	G

MLMATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGACRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHDCPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRYYKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRKWDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLKDRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGEENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALAVRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAPVEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSLEVIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVVLENDLHRHASAIRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNEGRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPELAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEGNNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTSVPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGTRVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ

Source Source

Source organism organism	Escherichia coli BW25113
NCBI taxonomy ID ncbi_taxonomy_id	679895
Host organism host_organism	Escherichia coli BW25113

Entity 4 Entity 4 polymer

Description description	NADH-quinone oxidoreductase subunit I
Molecular weight formula_weight	20562.8 kDa
EC 编号 ec_number	7.1.1.-
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	I

MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRYRGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFCGLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDGKDKGEAENEAKPIDVKSLLP

Source Source

Source organism organism	Escherichia coli BL21(DE3)
NCBI taxonomy ID ncbi_taxonomy_id	469008
Host organism host_organism	Escherichia coli BW25113

Entity 5 Entity 5 polymer

Description description	NADH-quinone oxidoreductase subunit B
Molecular weight formula_weight	25081.8 kDa
EC 编号 ec_number	7.1.1.-
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	B

MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLNDMVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTCFTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPPRPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPDEI

Source Source

Source organism organism	Escherichia coli BW25113
NCBI taxonomy ID ncbi_taxonomy_id	679895
Host organism host_organism	Escherichia coli BW25113

Entity 6 Entity 6 polymer

Description description	NADH-quinone oxidoreductase subunit C/D
Molecular weight formula_weight	68780.5 kDa
EC 编号 ec_number	7.1.1.-
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	C

MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATRTGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHLISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMMPQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFLNLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIEYLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMTPVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLASYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENFDFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERTLQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRVRTPSFAHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR

Source Source

Source organism organism	Escherichia coli BL21(DE3)
NCBI taxonomy ID ncbi_taxonomy_id	469008
Host organism host_organism	Escherichia coli BW25113

Entity 7 Entity 7 polymer

Description description	NADH-quinone oxidoreductase subunit H
Molecular weight formula_weight	36240.9 kDa
EC 编号 ec_number	7.1.1.-
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	H

MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQNRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVPVSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFLGLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAEQELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTAFFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ

Source Source

Source organism organism	Escherichia coli BW25113
NCBI taxonomy ID ncbi_taxonomy_id	679895
Host organism host_organism	Escherichia coli BW25113

Entity 8 Entity 8 polymer

Description description	NADH-quinone oxidoreductase subunit J
Molecular weight formula_weight	19889.6 kDa
EC 编号 ec_number	7.1.1.-
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	J

MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFFSLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAIMLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGREERAGEVLSNRKDDSAKRKTEEHA

Source Source

Source organism organism	Escherichia coli BW25113
NCBI taxonomy ID ncbi_taxonomy_id	679895
Host organism host_organism	Escherichia coli BW25113

Entity 9 Entity 9 polymer

Description description	NADH-quinone oxidoreductase subunit K
Molecular weight formula_weight	10853.0 kDa
EC 编号 ec_number	7.1.1.-
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	K

MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASALAFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG

Source Source

Source organism organism	Escherichia coli BW25113
NCBI taxonomy ID ncbi_taxonomy_id	679895
Host organism host_organism	Escherichia coli BW25113

Entity 10 Entity 10 polymer

Description description	NADH-quinone oxidoreductase subunit M
Molecular weight formula_weight	56560.1 kDa
EC 编号 ec_number	7.1.1.-
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	M

MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSLQLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAVLCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHKASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSGVEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSLPLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGSQLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFAVATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKSQIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTRP

Source Source

Source organism organism	Escherichia coli BW25113
NCBI taxonomy ID ncbi_taxonomy_id	679895
Host organism host_organism	Escherichia coli BW25113

Entity 11 Entity 11 polymer

Description description	NADH-quinone oxidoreductase subunit A
Molecular weight formula_weight	16474.3 kDa
EC 编号 ec_number	7.1.1.-
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A

MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARARSKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVEAAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR

Source Source

Source organism organism	Escherichia coli BW25113
NCBI taxonomy ID ncbi_taxonomy_id	679895
Host organism host_organism	Escherichia coli BW25113

Entity 12 Entity 12 polymer

Description description	NADH-quinone oxidoreductase subunit L
Molecular weight formula_weight	66483.6 kDa
EC 编号 ec_number	7.1.1.-
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	L

MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALVTAFIGVDFFANGEQTYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMYASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTDPKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWATLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMTPEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFHLMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLVTAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKGVTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLAAWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLNSMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR

Source Source

Source organism organism	Escherichia coli BW25113
NCBI taxonomy ID ncbi_taxonomy_id	679895
Host organism host_organism	Escherichia coli BW25113

Entity 13 Entity 13 polymer

Description description	NADH-quinone oxidoreductase subunit N
Molecular weight formula_weight	52072.7 kDa
EC 编号 ec_number	7.1.1.-
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	N

MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIGLNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNKDEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYTILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKLSLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAIIAFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYLFSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGFIGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQYSAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM

Source Source

Source organism organism	Escherichia coli BW25113
NCBI taxonomy ID ncbi_taxonomy_id	679895
Host organism host_organism	Escherichia coli BW25113

Entity 14 Entity 14 non-polymer

Description description	FE2/S2 (INORGANIC) CLUSTER
Molecular weight formula_weight	175.8 kDa
Source method src_method	syn
Copy count entity_count	2

Entity 15 Entity 15 non-polymer

Description description	CALCIUM ION
Molecular weight formula_weight	40.1 kDa
Source method src_method	syn
Copy count entity_count	5

Entity 16 Entity 16 non-polymer

Description description	FLAVIN MONONUCLEOTIDE
Molecular weight formula_weight	456.3 kDa
Source method src_method	syn
Copy count entity_count	1

Entity 17 Entity 17 non-polymer

Description description	IRON/SULFUR CLUSTER
Molecular weight formula_weight	351.6 kDa
Source method src_method	syn
Copy count entity_count	7

Entity 18 Entity 18 non-polymer

Description description	1,2-Distearoyl-sn-glycerophosphoethanolamine
Molecular weight formula_weight	748.1 kDa
Source method src_method	syn
Copy count entity_count	16

Entity 19 Entity 19 non-polymer

Description description	EICOSANE
Molecular weight formula_weight	282.5 kDa
Source method src_method	syn
Copy count entity_count	2

Entity 20 Entity 20 non-polymer

Description description	CARDIOLIPIN
Molecular weight formula_weight	1464.0 kDa
Source method src_method	syn
Copy count entity_count	1

Entity 21 Entity 21 non-polymer

Description description	TRIDECANE
Molecular weight formula_weight	184.4 kDa
Source method src_method	syn
Copy count entity_count	3

Entity 22 Entity 22 non-polymer

Description description	Ubiquinone-8
Molecular weight formula_weight	727.1 kDa
Source method src_method	syn
Copy count entity_count	2

Entity 23 Entity 23 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	428

▶

Unit-cell length a length_a	1.000 Å
Unit-cell length b length_b	1.000 Å
Unit-cell length c length_c	1.000 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	—

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	triclinic

Resolution resolution	3.00 Å
Resolution测定Method resolution_method	—
颗粒数 num_particles	70502

E. coli Complex I WT purified in LMNG

1. Protein Identity and Related Structures Protein Identity & Related Structures

NADH-quinone oxidoreductase subunit E

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

NADH-quinone oxidoreductase subunit F

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

NADH-quinone oxidoreductase subunit G

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

NADH-quinone oxidoreductase subunit I

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

NADH-quinone oxidoreductase subunit B

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

NADH-quinone oxidoreductase subunit C/D

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

NADH-quinone oxidoreductase subunit H

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

NADH-quinone oxidoreductase subunit J

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

NADH-quinone oxidoreductase subunit K

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

NADH-quinone oxidoreductase subunit M

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer