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9u4v

Cryo-EM map of MSMEG_3496 in complex with AcpM, size exclusion chromatography peak2

Method: ELECTRON MICROSCOPY D_max: 120.8 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9u4v
deposition_date	2025-03-20
title	Cryo-EM map of MSMEG_3496 in complex with AcpM, size exclusion chromatography peak2
keywords	Efflux pump MSMEG_3496-AcpM complex, MEMBRANE PROTEIN; MEMBRANE PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	34.38 Å
rg_electron	34.08 Å
i0	114230000.00
molecular_weight	89548.0 kDa
excluded_volume	113810 Å³
envelope_volume	136210 Å³
shell_volume	35738 Å³
envelope_diameter	128.0 Å
shell_rg	37.68 Å
envelope_rg	34.78 Å
shape_rg	34.14 Å
total_rg	34.14 Å
total_atoms	6301
n_residues	824
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	120.8 Å
rg_real	34.76 Å
rg_real_error	1.33 Å
i0_real	1.1420e+08
i0_real_error	2.0480e+06
rg_reciprocal	34.52 Å
i0_reciprocal	114200000.0000
total_estimate	0.7935
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	28.9 Å
skewness	0.605
kurtosis	-0.281
angular_range	— – 0.2300 Å⁻¹
current_alpha	0.0000
highest_alpha	32730000.0000
n_real_points	47
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.591; Stabil: 0.999; Sysdev: 1.000; Positv: 1.000; Valcen: 0.687; Smooth: 0.854

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4. Crystallography & Experiment

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5. Entities & Polymer Info (2)

Entity 1 polymer

description	MmpL4 protein
formula_weight	110357.6 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	6301

MSNPSHDAPTDAMGVSGPKHARPGGIAKWIRVLAVPIILVWVAIIAVLNTVVPQLDVVGEMRSVSMSPDDAPSVIAMKRVGEVFEEFKSNSSVMIVLEGEQPLGDEAHKYYDEIVDKLEADPAHVEHVQDFWGDPLTASGAQSPDGLASYVQVYTRGNQGEALANESVEAVQDIVESVPAPPGVKAYVTGPAALSADQHVASDRSVRVIEALTFAVIITMLLLVYRSIVTVILTLVMVVLSLSAARGMIAFLGYHEIIGLSVFATNLLTTLAIAAATDYAIFLIGRYQEARSVGEDREQSYYTMFHSTAHVVLGSGMTIAGATLCLHFTRMPYFQSLGIPLAIGMSVVVLASLTMGAAIISVASRFGKTFEPKRAMRTRGWRKLGAAVVRWPAPILVTTIALSVVGLLALPGYQTNYNDRRYLPQDLPANTGYAAADRHFSQARMNPELLMIESDHDLRNSADFLVVDRIAKRVFQVPGISRVQAITRPQGTPIEHTSIPFQISMSGTTQMMNMKYMQDRMADMLVMADEMQKSVDTMEEMLKITREMSDTTHSMVGKMHGMVEDIKELRDHIADFDDFLRPIRNYFYWEPHCDSIPVCQSIRSIFDTLDGIDVMTDDIQRLMPDMDRLDELMPQMLTIMPPMIESMKTMKTMMLTTQATMGGLQDQMEAAMENQTAMGQAFDASKNDDSFYLPPETFENPDFKRGMKMFLSPDGHAVRFIISHEGDPMSPEGIKHIDAIKQAAKEAIKGTPLEGSKIYLGGTAATFKDLQEGANYDLIIAGIAALCLIFIIMLIITRAVVASAVIVGTVVISLGASFGLSVLIWQHIIGLELHWMVLAMAVIVLLAVGADYNLLLVSRIKEEIHAGLNTGIIRSMGGTGSVVTSAGLVFAFTMMSMAVSELAVIAQVGTTIGLGLLFDTLVIRSFMTPSIAALMGKWFWWPQRVRQRPLPAPWPQPVQRDPEDALNRVAAAENLYFQGLWSHPQFEKGGGSGGGSGGSAWSHPQFEK

Source

organism	Mycolicibacterium smegmatis MC2 155
ncbi_taxonomy_id	246196
host_organism	Mycolicibacterium smegmatis MC2 155

Entity 2 polymer

description	Meromycolate extension acyl carrier protein
formula_weight	11084.2 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	B

MAATQEEIIAGLAEIIEEVTGIEPSEVTPEKSFVDDLDIDXLSMVEIAVQTEDKYGVKIPDEDLAGLRTVGDVVAYIQKLEEENPEAAAALREKFAADQ

Source

organism	Mycolicibacterium smegmatis MC2 155
ncbi_taxonomy_id	246196
host_organism	Mycolicibacterium smegmatis MC2 155

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	triclinic

resolution	3.09 Å
resolution_method	—
num_particles	1419524

fit	/app/data/saxs_computation/u4/9u4v/9u4v.fit
pddf	/app/data/pr_computation/u4/9u4v/9u4v.pr.out
profile	/app/data/saxs_computation/u4/9u4v/9u4v.dat

Cryo-EM map of MSMEG_3496 in complex with AcpM, size exclusion chromatography peak2

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

EM 3D

5. Entities & Polymer Info (2)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)