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9u94

Cryo-EM structure of Fusobacterium nucleatum CbpF in complex with human CEACAM1

Method: ELECTRON MICROSCOPY D_max: 128.8 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9u94
deposition_date	2025-03-27
title	Cryo-EM structure of Fusobacterium nucleatum CbpF in complex with human CEACAM1
keywords	Fusobacterium nucleatum, trimeric autotransporter adhesin, CbpF, CEACAM1, CELL ADHESION; CELL ADHESION
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	36.55 Å
rg_electron	37.06 Å
i0	234225000.00
molecular_weight	117730.0 kDa
excluded_volume	144710 Å³
envelope_volume	188960 Å³
shell_volume	45478 Å³
envelope_diameter	133.5 Å
shell_rg	40.41 Å
envelope_rg	38.00 Å
shape_rg	37.03 Å
total_rg	37.35 Å
total_atoms	8316
n_residues	1110
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	128.8 Å
rg_real	36.81 Å
rg_real_error	1.22 Å
i0_real	2.3420e+08
i0_real_error	4.6120e+06
rg_reciprocal	36.65 Å
i0_reciprocal	234200000.0000
total_estimate	0.8437
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	38.9 Å
skewness	0.566
kurtosis	0.034
angular_range	— – 0.2150 Å⁻¹
current_alpha	0.0000
highest_alpha	34090000.0000
n_real_points	44
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.763; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.840; Smooth: 0.835

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4. Crystallography & Experiment

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5. Entities & Polymer Info (2)

Entity 1 polymer

description	Fusobacterium nucleatum CbpF
formula_weight	35874.6 kDa
src_method	man
entity_count	3

Polymer

type	polypeptide(L)
strand_id	C,A,B
atom_count	8316

MVSFSAPVFQAGTGTDSTVAGVNNEANGEKSSAFGYENKAKEKLSSAFGYKNIANGIEGSAFGISNLAKGQYSSAFGFRNVANKRHSSAFGSGNEANGEQSSAFGFKNTVSGFNSSAFGSQYEVTGNFSGAFGMGEFNGQYQYKNEGNNSYMIGNKNKIASGSNDNFILGNNVHIGGGINNSVALGNNSTVSASNTVSVGSSTLKRKIVNVGDGAISANSSDAVTGRQLYSGNGIDTAAWQNKLNVTRKNDYKDANDIDVNKWKAKLGVGSGGGGGTADIENLRNEVNEKIDDVKDEVRENLYFQGSAWSHPQFEKGGGSGGGSGGSAWSHPQFEKHHHHHH

Source

organism	Fusobacterium nucleatum
ncbi_taxonomy_id	851
host_organism	Escherichia coli

Entity 2 polymer

description	Cell adhesion molecule CEACAM1
formula_weight	15913.4 kDa
src_method	man
entity_count	3

Polymer

type	polypeptide(L)
strand_id	F,E,D

MQLTTESMPFNVAEGKEVLLLVHNLPQQLFGYSWYKGERVDGNRQIVGYAIGTQQATPGPANSGRETIYPNASLLIQNVTQNDTGFYTLQVIKSDLVNEEATGQFHVYENLYFQGSAWSHPQFEKGGGSGGGSGGSAWSHPQFEK

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Escherichia coli

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	2.110 Å
resolution_low	— Å
r_factor	—
r_work	—
r_free	—

resolution	2.11 Å
resolution_method	—
num_particles	430775

fit	/app/data/saxs_computation/u9/9u94/9u94.fit
pddf	/app/data/pr_computation/u9/9u94/9u94.pr.out
profile	/app/data/saxs_computation/u9/9u94/9u94.dat

Cryo-EM structure of Fusobacterium nucleatum CbpF in complex with human CEACAM1

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

EM 3D

5. Entities & Polymer Info (2)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)