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9ufn

Phage T4 hub in post-tail contraction, pre-genome-release state

Method: ELECTRON MICROSCOPY D_max: 196.3 Å Quality: REASONABLE

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9ufn
deposition_date	2025-04-10
title	Phage T4 hub in post-tail contraction, pre-genome-release state
keywords	Bacteriophage, Myovirus, Phage T4, tail contraction, phage hub complex, tape-measure protein, genome release, VIRAL PROTEIN; VIRAL PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	53.54 Å
rg_electron	54.93 Å
i0	103857000.00
molecular_weight	81800.0 kDa
excluded_volume	101860 Å³
envelope_volume	194080 Å³
shell_volume	34620 Å³
envelope_diameter	208.7 Å
shell_rg	44.49 Å
envelope_rg	55.76 Å
shape_rg	54.94 Å
total_rg	54.35 Å
total_atoms	5769
n_residues	725
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	196.3 Å
rg_real	54.58 Å
rg_real_error	2.77 Å
i0_real	1.0390e+08
i0_real_error	1.9280e+06
rg_reciprocal	52.68 Å
i0_reciprocal	103600000.0000
total_estimate	0.7018
solution_quality	REASONABLE a REASONABLE solution
n_peaks	2
r_peak_primary	40.6 Å
skewness	0.704
kurtosis	-0.192
angular_range	— – 0.1450 Å⁻¹
current_alpha	0.0000
highest_alpha	7359000.0000
n_real_points	30
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.384; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.285; Smooth: 0.683

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	Tail tube protein gp19
formula_weight	18479.6 kDa
src_method	nat
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B
atom_count	5769

MFVDDVTRAFESGDFARPNLFQVEISYLGQNFTFQCKATALPAGIVEKIPVGFMNRKINVAGDRTFDDWTVTVMNDEAHDARQKFVDWQSIAAGQGNEITGGKPAEYKKSAIVRQYARDAKTVTKEIEIKGLWPTNVGELQLDWDSNNEIQTFEVTLALDYWE

Entity 2 polymer

description	Baseplate tail-tube junction protein gp54
formula_weight	35015.2 kDa
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	C

MYSLEEFNNQAINADFQRNNMFSCVFATTPSTKSSSLISSISNFSYNNLGLNSDWLGLTQGDINQGITTLITAGTQKLIRKSGVSKYLIGAMSQRTVQSLLGSFTVGTYLIDFFNMAYNSSGLMIYSVKMPENRLSYETDWNYNSPNIRITGRELDPLVISFRMDSEACNYRAMQDWVNSVQDPVTGLRALPQDVEADIQVNLHSRNGLPHTAVMFTGCIPISVSAPELSYDGDNQITTFDVTFAYRVMQAGAVDRQRALEWLESAAINGIQSVLGNSGGVTGLSNSLSRLSRLGGTAGSISNINTMTGIVNSQSKILGAI

Entity 3 polymer

description	Baseplate tail-tube junction protein gp48
formula_weight	39770.3 kDa
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	D

MAIVKEITADLIKKSGEKISAGQSTKSEVGTKTYTAQFPTGRASGNDTTEDFQVTDLYKNGLLFTAYNMSSRDSGSLRSMRSNYSSSSSSILRTARNTISSTVSKLSNGLISNNNSGTISKSPIANILLPRSKSDVDTSSHRFNDVQESLISRGGGTATGVLSNIASTAVFGALESITQGIMADNNEQIYTTARSMYGGAENRTKVFTWDLTPRSTEDLMAIINIYQYFNYFSYGETGKSQYAAEIKGYLDDWYRSTLIEPLSPEDAAKNKTLFEKMTSSLTNVLVVSNPTVWMVKNFGATSKFDGKTEIFGPCQIQSIRFDKTPNGNFNGLAIAPNLPSTFTLEITMREIITLNRASLYAGTF

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6. Citations (1)

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resolution	3.80 Å
resolution_method	—
num_particles	36951

fit	/app/data/saxs_computation/uf/9ufn/9ufn.fit
pddf	/app/data/pr_computation/uf/9ufn/9ufn.pr.out
profile	/app/data/saxs_computation/uf/9ufn/9ufn.dat

Phage T4 hub in post-tail contraction, pre-genome-release state

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

EM 3D

5. Entities & Polymer Info (3)

Entity 1 polymer

Polymer

Entity 2 polymer

Polymer

Entity 3 polymer

Polymer

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)