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9unv

Cryo-EM structure of human organic solute transporter Ost-alpha/beta bound with TLCA

Method: ELECTRON MICROSCOPY D_max: 92.1 Å Quality: REASONABLE

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9unv
deposition_date	2025-04-24
title	Cryo-EM structure of human organic solute transporter Ost-alpha/beta bound with TLCA
keywords	Complex, bile acids, TRANSPORT PROTEIN; TRANSPORT PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	29.40 Å
rg_electron	28.39 Å
i0	238705000.00
molecular_weight	92507.0 kDa
excluded_volume	95182 Å³
envelope_volume	154070 Å³
shell_volume	43461 Å³
envelope_diameter	98.2 Å
shell_rg	37.12 Å
envelope_rg	28.54 Å
shape_rg	28.35 Å
total_rg	29.13 Å
total_atoms	7068
n_residues	748
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	92.1 Å
rg_real	29.27 Å
rg_real_error	0.68 Å
i0_real	2.3870e+08
i0_real_error	3.8930e+06
rg_reciprocal	29.33 Å
i0_reciprocal	238700000.0000
total_estimate	0.6923
solution_quality	REASONABLE a REASONABLE solution
n_peaks	2
r_peak_primary	37.5 Å
skewness	0.182
kurtosis	-0.436
angular_range	— – 0.2700 Å⁻¹
current_alpha	0.0000
highest_alpha	46340000.0000
n_real_points	55
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.921; Stabil: 1.000; Sysdev: 0.092; Positv: 1.000; Valcen: 0.991; Smooth: 0.963

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4. Crystallography & Experiment

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5. Entities & Polymer Info (9)

Entity 1 polymer

description	Organic solute transporter subunit alpha
formula_weight	38967.1 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,C
atom_count	7068

MEPGRTQIKLDPRYTADLLEVLKTNYGIPSACFSQPPTAAQLLRALGPVELALTSILTLLALGSIAIFLEDAVYLYKNTLCPIKRRTLLWKSSAPTVVSVLCCFGLWIPRSLVLVEMTITSFYAVCFYLLMLVMVEGFGGKEAVLRTLRDTPMMVHTGPCCCCCPCCPRLLLTRKKLQLLMLGPFQYAFLKITLTLVGLFLVPDGIYDPADISEGSTALWINTFLGVSTLLALWTLGIISRQARLHLGEQNMGAKFALFQVLLILTALQPSIFSVLANGGQIACSPPYSSKTRSQVMNCHLLILETFLMTVLTRMYYRRKDHKVGYETFSSPDLDLNLKAGGSDYKDDDDK

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Homo sapiens

Entity 2 polymer

description	Organic solute transporter subunit beta
formula_weight	15943.9 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	B,D

MEHSEGAPGDPAGTVVPQELLEEMLWFFRVEDASPWNHSILALAAVVVIISMVLLGRSIQASRKEKMQPPEKETPEVLHLDEAKDHNSLNNLRETLLSEKPNLAQVELELKERDVLSVFLPDVPETESGGSHHHHHHHHHH

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Homo sapiens

Entity 3 non-polymer

description	CHOLESTEROL
formula_weight	386.7 kDa
src_method	syn
entity_count	26

Entity 4 non-polymer

description	PHOSPHATIDYL ETHANOL
formula_weight	717.0 kDa
src_method	syn
entity_count	2

Entity 5 non-polymer

description	1-O-OCTADECYL-SN-GLYCERO-3-PHOSPHOCHOLINE
formula_weight	510.7 kDa
src_method	syn
entity_count	2

Entity 6 non-polymer

description	2-acetamido-2-deoxy-beta-D-glucopyranose
formula_weight	221.2 kDa
src_method	syn
entity_count	2

Entity 7 non-polymer

description	PALMITIC ACID
formula_weight	256.4 kDa
src_method	syn
entity_count	14

Entity 8 non-polymer

description	Taurolithocholic Acid
formula_weight	483.7 kDa
src_method	syn
entity_count	2

Entity 9 non-polymer

description	(7E,21R,24S)-27-amino-24-hydroxy-18,24-dioxo-19,23,25-trioxa-24lambda~5~-phosphaheptacos-7-en-21-yl (9Z,12E)-octadeca-9,12-dienoate
formula_weight	742.0 kDa
src_method	syn
entity_count	4

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6. Citations (1)

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resolution	3.12 Å
resolution_method	—
num_particles	76705

fit	/app/data/saxs_computation/un/9unv/9unv.fit
pddf	/app/data/pr_computation/un/9unv/9unv.pr.out
profile	/app/data/saxs_computation/un/9unv/9unv.dat

Cryo-EM structure of human organic solute transporter Ost-alpha/beta bound with TLCA

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

EM 3D

5. Entities & Polymer Info (9)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 non-polymer

Entity 4 non-polymer

Entity 5 non-polymer

Entity 6 non-polymer

Entity 7 non-polymer

Entity 8 non-polymer

Entity 9 non-polymer

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)